Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,476,619	A→G	V91A (GTG→GCG)	b4256 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,476,619	0	A	G	99.6%	784.2 / ‑5.2	245	V91A (GTG→GCG)	b4256	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (113/130); total (113/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 252 total aligned reads displayed.

ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAA  >  NC_000913/4476529‑4476707

 tCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCtt                                                                                       >  1:3223805/1‑93 (MQ=255)

  ccGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTc                                                                                      <  2:5822467/93‑1 (MQ=255)

  ccGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTc                                                                                      >  2:6228779/1‑93 (MQ=255)

   cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTGAGGTTCGCGCAACATCCGCGCTTca                                                                                     >  1:163246/1‑93 (MQ=255)

   cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTca                                                                                     >  2:4265018/1‑93 (MQ=255)

    ggCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTcac                                                                                    >  2:787220/1‑93 (MQ=255)

    ggCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTcac                                                                                    >  1:5148085/1‑93 (MQ=255)

      cATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACaa                                                                                  >  1:4159038/1‑93 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                <  2:1472144/93‑1 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                >  2:3916037/1‑93 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                >  1:5923815/1‑93 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                <  2:3475191/93‑1 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                <  2:2405711/93‑1 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                >  2:4934864/1‑93 (MQ=255)

        tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATc                                                                                >  2:2610173/1‑93 (MQ=255)

          aaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCgc                                                                              >  2:4395753/1‑92 (MQ=255)

          aaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGa                                                                              >  1:2724639/1‑93 (MQ=255)

            cAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACa                                                                            <  2:3693730/93‑1 (MQ=255)

            cAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACa                                                                            >  2:285782/1‑93 (MQ=255)

             aaaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAt                                                                           >  1:3052519/1‑93 (MQ=255)

             aaaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAt                                                                           <  2:5759922/93‑1 (MQ=255)

             aaaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAt                                                                           >  1:2466010/1‑93 (MQ=255)

              aaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATg                                                                          <  1:5756691/93‑1 (MQ=255)

              aaaCGTACGTGGTTAATGTGCGAAGGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATg                                                                          <  1:4424426/93‑1 (MQ=255)

                acgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGc                                                                        <  1:3124961/93‑1 (MQ=255)

                acgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGc                                                                        <  2:3182307/93‑1 (MQ=255)

                 cgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGcc                                                                       >  1:1015631/1‑93 (MQ=255)

                 cgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGcc                                                                       <  2:2421127/93‑1 (MQ=255)

                    acgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGt                                                                    <  2:5505967/93‑1 (MQ=255)

                     cgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTg                                                                   <  1:2665215/93‑1 (MQ=255)

                     cgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTg                                                                   <  2:229428/93‑1 (MQ=255)

                      gttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGc                                                                  >  1:516703/1‑93 (MQ=255)

                       ttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCa                                                                 <  1:5267552/93‑1 (MQ=255)

                            aaTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCaa                                                            <  1:4805234/93‑1 (MQ=255)

                            aaTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGACCGTGCAGCCaa                                                            >  2:5250908/1‑93 (MQ=255)

                             aTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAg                                                           <  1:1958089/93‑1 (MQ=255)

                             aTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAg                                                           <  2:3950802/93‑1 (MQ=255)

                             aTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAg                                                           >  2:296870/1‑93 (MQ=255)

                              tGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCCAgc                                                          >  1:5459690/1‑93 (MQ=255)

                              tGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAgc                                                          >  1:809252/1‑93 (MQ=255)

                               gTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAgcg                                                         <  1:5410793/93‑1 (MQ=255)

                                   gAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                     <  1:4296257/93‑1 (MQ=255)

                                   gAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                     <  2:2100455/93‑1 (MQ=255)

                                   gAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                     >  1:6261944/1‑93 (MQ=255)

                                    aaTGCCCGCGAGTTGAACCCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAg                                                    <  1:1526087/93‑1 (MQ=255)

                                     aTGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGt                                                   <  1:3606552/93‑1 (MQ=255)

                                     aTGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGt                                                   <  2:1050160/93‑1 (MQ=255)

                                     aTGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGt                                                   <  2:4648037/93‑1 (MQ=255)

                                      tGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTc                                                  >  1:86900/1‑93 (MQ=255)

                                        cccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                <  2:6274184/93‑1 (MQ=255)

                                         ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGat                                               >  1:1910413/1‑93 (MQ=255)

                                         ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGat                                               <  1:2331804/93‑1 (MQ=255)

                                          cgcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGata                                              <  1:5514078/93‑1 (MQ=255)

                                           gcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGatat                                             <  1:6050675/93‑1 (MQ=255)

                                           gcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGatat                                             <  2:5888400/93‑1 (MQ=255)

                                           gcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGatat                                             <  2:6141454/93‑1 (MQ=255)

                                             gAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc                                           >  2:3771394/1‑93 (MQ=255)

                                                 tGACCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaa                                       <  1:4312414/93‑1 (MQ=255)

                                                 tGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaa                                       >  1:2421118/1‑93 (MQ=255)

                                                 tGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaa                                       <  1:4192553/93‑1 (MQ=255)

                                                   aaCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAg                                     >  1:2222553/1‑93 (MQ=255)

                                                    acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                    >  1:6114172/1‑93 (MQ=255)

                                                    acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                    <  2:5451510/93‑1 (MQ=255)

                                                    acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                    <  1:2786190/93‑1 (MQ=255)

                                                       cgcgGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCa                                 <  2:3852382/93‑1 (MQ=255)

                                                        gcgGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaa                                >  2:60682/1‑93 (MQ=255)

                                                        gcgGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaa                                >  2:4813156/1‑93 (MQ=255)

                                                         cgGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                               <  1:690084/93‑1 (MQ=255)

                                                           ggCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATg                             >  1:1402677/1‑93 (MQ=255)

                                                            gCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGc                            <  2:1803856/93‑1 (MQ=255)

                                                            gCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCAATATGCTCAAAGCCCAAATGc                            <  1:564620/93‑1 (MQ=255)

                                                             cATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCt                           <  1:5988897/93‑1 (MQ=255)

                                                               tttttttAGAGTTCGCGCAACATCCGCTCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCa                         <  2:2735032/93‑1 (MQ=255)

                                                               tttttttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCa                         <  2:454722/93‑1 (MQ=255)

                                                               tttttttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCa                         <  2:4208209/93‑1 (MQ=255)

                                                                ttttttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAt                        >  1:3904895/1‑93 (MQ=255)

                                                                   tttAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                     <  2:1412423/93‑1 (MQ=255)

                                                                       gagTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAgcgc                 <  1:1181545/93‑1 (MQ=255)

                                                                        agTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCa                <  2:4429786/93‑1 (MQ=255)

                                                                         gTTCGCGCACCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCaa               <  1:739620/93‑1 (MQ=255)

                                                                         gTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCaa               <  1:338815/93‑1 (MQ=255)

                                                                         gTTCGCGCAACATCAGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCaa               <  1:2959906/93‑1 (MQ=255)

                                                                          ttCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt              <  1:5160334/93‑1 (MQ=255)

                                                                          ttCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt              >  1:4645901/1‑93 (MQ=255)

                                                                          ttCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt              <  2:2059867/93‑1 (MQ=255)

                                                                           tCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATg             <  2:235780/93‑1 (MQ=255)

                                                                           tCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATg             >  1:4001413/1‑93 (MQ=255)

                                                                            cgcgCAACAGCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATgg            <  1:1288449/93‑1 (MQ=255)

                                                                             gcgcAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGc           <  2:1177796/93‑1 (MQ=255)

                                                                             gcgcAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGc           <  2:5640424/93‑1 (MQ=255)

                                                                               gcAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCtt         >  2:3085619/1‑93 (MQ=255)

                                                                                cAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTc        >  1:1663867/1‑93 (MQ=255)

                                                                                 aaCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTcc       <  2:3558939/93‑1 (MQ=255)

                                                                                  aCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt      <  1:2926159/93‑1 (MQ=255)

                                                                                  aCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt      >  1:1101637/1‑93 (MQ=255)

                                                                                   cATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCtt     >  1:4505757/1‑93 (MQ=255)

                                                                                   cATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCtt     >  2:1826220/1‑93 (MQ=255)

                                                                                   aaTCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCtt     <  1:4566788/92‑1 (MQ=255)

                                                                                    aTCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCttt    >  2:5038578/1‑93 (MQ=255)

                                                                                      ccGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCACAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaa  >  1:2597147/1‑93 (MQ=255)

ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAA  >  NC_000913/4476529‑4476707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  >  NC_000913/4476526‑4476712

CACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCG                                                                                               <  SRR4302533.1000901/94‑1 (MQ=60)

  CATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCT                                                                                             >  SRR4302533.1864195/1‑94 (MQ=60)

   ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTT                                                                                            >  SRR4302533.5750989/1‑94 (MQ=60)

   ATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTT                                                                                            >  SRR4302533.3243683/1‑94 (MQ=60)

     CCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTGAGGTTCGCGCAACATCCGCGCTTCA                                                                                          >  SRR4302533.164286/1‑94 (MQ=60)

      CGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCAC                                                                                         >  SRR4302533.5179190/1‑94 (MQ=60)

        GCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAA                                                                                       >  SRR4302533.4184242/1‑94 (MQ=60)

         CATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAAT                                                                                      >  SRR4302533.6061204/1‑94 (MQ=60)

         CATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAAT                                                                                      >  SRR4302533.5548200/1‑94 (MQ=60)

         CATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAAT                                                                                      <  SRR4302533.4566560/94‑1 (MQ=60)

          ATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATC                                                                                     >  SRR4302533.5959621/1‑94 (MQ=60)

          ATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATC                                                                                     <  SRR4302533.2553742/94‑1 (MQ=60)

          ATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATC                                                                                     <  SRR4302533.1139042/94‑1 (MQ=60)

           TAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCG                                                                                    <  SRR4302533.1605037/94‑1 (MQ=60)

            AAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGA                                                                                   >  SRR4302533.2741356/1‑94 (MQ=60)

             aaaaaaagtgagGTTGTTAATGTCCGAATGCCCGCGAGTTGAACACGCGGCCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGAC                                                                                  <  SRR4302533.3342780/82‑1 (MQ=60)

             AACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGAC                                                                                  <  SRR4302533.6024430/94‑1 (MQ=60)

              ACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGCACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACA                                                                                 >  SRR4302533.4580856/1‑94 (MQ=60)

               CAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAT                                                                                >  SRR4302533.2481222/1‑94 (MQ=60)

               CAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAT                                                                                <  SRR4302533.3120824/94‑1 (MQ=60)

               CAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACAT                                                                                >  SRR4302533.3071215/1‑94 (MQ=60)

                 AAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGG                                                                              <  SRR4302533.1316131/94‑1 (MQ=60)

                 AAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGG                                                                              <  SRR4302533.5791430/94‑1 (MQ=60)

                 AAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGG                                                                              >  SRR4302533.4525431/1‑94 (MQ=60)

                 AAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGG                                                                              <  SRR4302533.4948448/94‑1 (MQ=60)

                 AAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGG                                                                              <  SRR4302533.344582/94‑1 (MQ=60)

                 AAACGTACGTGGTTAATGTGCGAAGGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGG                                                                              <  SRR4302533.4451170/94‑1 (MQ=60)

                   ACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCC                                                                            <  SRR4302533.3144168/94‑1 (MQ=60)

                   ACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCC                                                                            >  SRR4302533.3608815/1‑94 (MQ=60)

                   ACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCC                                                                            >  SRR4302533.1021598/1‑94 (MQ=60)

                     GTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCAAAATCGACATGGCCCG                                                                          >  SRR4302533.5198203/1‑94 (MQ=60)

                       ACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTG                                                                        >  SRR4302533.5490161/1‑94 (MQ=60)

                       ACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTG                                                                        <  SRR4302533.6323072/94‑1 (MQ=60)

                        CGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGC                                                                       >  SRR4302533.519769/1‑94 (MQ=60)

                        CGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGC                                                                       >  SRR4302533.714502/1‑94 (MQ=60)

                        CGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGC                                                                       <  SRR4302533.2681571/94‑1 (MQ=60)

                         GTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCA                                                                      <  SRR4302533.3656721/94‑1 (MQ=60)

                          TTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAG                                                                     <  SRR4302533.5271584/94‑1 (MQ=60)

                          TTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAG                                                                     <  SRR4302533.5299155/94‑1 (MQ=60)

                             TTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCA                                                                  >  SRR4302533.6235557/1‑94 (MQ=60)

                               AATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAG                                                                <  SRR4302533.5473641/94‑1 (MQ=60)

                               AATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAG                                                                <  SRR4302533.4834331/94‑1 (MQ=60)

                               AATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAG                                                                <  SRR4302533.816070/94‑1 (MQ=60)

                               AATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAG                                                                <  SRR4302533.812186/94‑1 (MQ=60)

                                ATGTTCGAATGCGCGCGAGTTTAACACGCGCGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGC                                                               <  SRR4302533.1969852/94‑1 (MQ=60)

                                ATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCCAGC                                                               >  SRR4302533.5492584/1‑94 (MQ=60)

                                ATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGC                                                               >  SRR4302533.3239820/1‑94 (MQ=60)

                                ATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGC                                                               >  SRR4302533.2851163/1‑94 (MQ=60)

                                ATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGC                                                               <  SRR4302533.1970042/94‑1 (MQ=60)

                                ATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGC                                                               >  SRR4302533.814002/1‑94 (MQ=60)

                                  GTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGC                                                             <  SRR4302533.5443324/94‑1 (MQ=60)

                                  GTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGC                                                             >  SRR4302533.93718/1‑94 (MQ=60)

                                   TTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCA                                                            <  SRR4302533.2707128/94‑1 (MQ=60)

                                    TCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCAT                                                           >  SRR4302533.4258044/1‑94 (MQ=60)

                                     CGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATA                                                          >  SRR4302533.1510098/1‑94 (MQ=60)

                                     CGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATA                                                          >  SRR4302533.4439978/1‑94 (MQ=60)

                                     CGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATA                                                          >  SRR4302533.6299379/1‑94 (MQ=60)

                                      GAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAG                                                         <  SRR4302533.3144599/94‑1 (MQ=60)

                                      GAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAG                                                         <  SRR4302533.4322095/94‑1 (MQ=60)

                                       AATGCCCGCGAGTTGAACCCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGT                                                        <  SRR4302533.1535272/94‑1 (MQ=60)

                                       AAGGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCGCGTGCAGCCAAGCGCATAGT                                                        <  SRR4302533.2418717/94‑1 (MQ=60)

                                        ATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTC                                                       >  SRR4302533.87459/1‑94 (MQ=60)

                                        ATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTC                                                       <  SRR4302533.758018/94‑1 (MQ=60)

                                        ATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTC                                                       <  SRR4302533.3628756/94‑1 (MQ=60)

                                         TGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCG                                                      <  SRR4302533.203007/94‑1 (MQ=60)

                                           CCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGAT                                                    >  SRR4302533.2991354/1‑94 (MQ=60)

                                           CCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGAT                                                    >  SRR4302533.1922067/1‑94 (MQ=60)

                                            CCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGGAACATCCGCGCTTCACAATCGACGTGGCCCGGGCAGCCAAGCGCATAGTCGATT                                                   >  SRR4302533.6167160/1‑94 (MQ=60)

                                            CCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATA                                                   <  SRR4302533.2346241/94‑1 (MQ=60)

                                             CGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATAT                                                  <  SRR4302533.5547367/94‑1 (MQ=60)

                                             CGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATAT                                                  <  SRR4302533.6131552/94‑1 (MQ=60)

                                             CGCGAGTTGAACACGCGGGCATTTTCTTAGAGTTGGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATAT                                                  <  SRR4302533.1170555/94‑1 (MQ=60)

                                              GCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATG                                                 <  SRR4302533.6087328/94‑1 (MQ=60)

                                              GCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATG                                                 <  SRR4302533.2666310/94‑1 (MQ=60)

                                               CGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGC                                                <  SRR4302533.1645488/94‑1 (MQ=60)

                                                GAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCT                                               >  SRR4302533.963361/1‑94 (MQ=60)

                                                GAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCT                                               <  SRR4302533.3347002/94‑1 (MQ=60)

                                                 AGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTC                                              >  SRR4302533.2442040/1‑94 (MQ=60)

                                                   TTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAA                                            >  SRR4302533.2436068/1‑94 (MQ=60)

                                                    TGACCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAA                                           <  SRR4302533.4338374/94‑1 (MQ=60)

                                                    TGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAA                                           <  SRR4302533.426948/94‑1 (MQ=60)

                                                    TGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAA                                           <  SRR4302533.4217929/94‑1 (MQ=60)

                                                     GAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAG                                          >  SRR4302533.2236283/1‑94 (MQ=60)

                                                     GAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAG                                          <  SRR4302533.1637242/94‑1 (MQ=60)

                                                      AACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGC                                         >  SRR4302533.6151066/1‑94 (MQ=60)

                                                       ACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCC                                        <  SRR4302533.2803307/94‑1 (MQ=60)

                                                        CCCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCC                                       <  SRR4302533.4403973/94‑1 (MQ=60)

                                                          CGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGGTTCACAATCGACATGGCCCGTGCAACCAAGTGCATAGTAGATGTGCTGCAAGCCCAA                                     >  SRR4302533.3269751/1‑94 (MQ=60)

                                                           GCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAA                                    <  SRR4302533.4733390/94‑1 (MQ=60)

                                                            CGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAAT                                   >  SRR4302533.4015345/1‑94 (MQ=60)

                                                            CGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAAT                                   <  SRR4302533.4330999/94‑1 (MQ=60)

                                                            CGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAAT                                   <  SRR4302533.5812530/94‑1 (MQ=60)

                                                            CGGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAAT                                   <  SRR4302533.694164/94‑1 (MQ=60)

                                                             GGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATG                                  >  SRR4302533.3661645/1‑94 (MQ=60)

                                                             GGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATG                                  >  SRR4302533.1411138/1‑94 (MQ=60)

                                                             GGGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATG                                  <  SRR4302533.5938045/94‑1 (MQ=60)

                                                              GGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGC                                 <  SRR4302533.6316027/94‑1 (MQ=60)

                                                              GGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGC                                 <  SRR4302533.2507165/94‑1 (MQ=60)

                                                              GGCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGC                                 >  SRR4302533.1870002/1‑94 (MQ=60)

                                                               GCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCT                                >  SRR4302533.6048414/1‑94 (MQ=60)

                                                               GCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCAATATGCTCAAAGCCCAAATGCT                                <  SRR4302533.567966/94‑1 (MQ=60)

                                                               GCATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCCTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATACT                                >  SRR4302533.4065329/1‑94 (MQ=60)

                                                                CATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTC                               <  SRR4302533.6025168/94‑1 (MQ=60)

                                                                 ATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCA                              >  SRR4302533.2365928/1‑94 (MQ=60)

                                                                 ATTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCA                              >  SRR4302533.6224891/1‑94 (MQ=60)

                                                                  TTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAT                             <  SRR4302533.2957073/94‑1 (MQ=60)

                                                                  TTTTTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAT                             >  SRR4302533.3928745/1‑94 (MQ=60)

                                                                     TTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAA                          >  SRR4302533.1207788/1‑94 (MQ=60)

                                                                     TTTTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAA                          <  SRR4302533.5529855/94‑1 (MQ=60)

                                                                       TTAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGC                        <  SRR4302533.4196884/94‑1 (MQ=60)

                                                                        TAGAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCG                       <  SRR4302533.1073184/94‑1 (MQ=60)

                                                                        TAGAGTTCGCGCAACATCCGCGCTTCACAAGCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCG                       <  SRR4302533.5607730/94‑1 (MQ=60)

                                                                          GAGTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCA                     <  SRR4302533.1188642/94‑1 (MQ=60)

                                                                            GTTCGCGCACCATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAT                   <  SRR4302533.743973/94‑1 (MQ=60)

                                                                            GTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAT                   >  SRR4302533.4674057/1‑94 (MQ=60)

                                                                            GTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAT                   <  SRR4302533.340880/94‑1 (MQ=60)

                                                                            GTTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGAGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAT                   >  SRR4302533.4765447/1‑94 (MQ=60)

                                                                            GTTCGCGCAACATCAGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAT                   <  SRR4302533.2977998/94‑1 (MQ=60)

                                                                             TTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATG                  >  SRR4302533.4025777/1‑94 (MQ=60)

                                                                             TTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATG                  >  SRR4302533.3060373/1‑94 (MQ=60)

                                                                             TTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATG                  <  SRR4302533.5191477/94‑1 (MQ=60)

                                                                             TTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATG                  <  SRR4302533.2857744/94‑1 (MQ=60)

                                                                             TTCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATG                  <  SRR4302533.2311277/94‑1 (MQ=60)

                                                                              TCGCGCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGG                 <  SRR4302533.4597325/94‑1 (MQ=60)

                                                                               CGCGCAACAGCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGC                <  SRR4302533.1296203/94‑1 (MQ=60)

                                                                                  GCAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTC             >  SRR4302533.1673925/1‑94 (MQ=60)

                                                                                   CAACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCC            <  SRR4302533.5311858/94‑1 (MQ=60)

                                                                                    AACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCT           <  SRR4302533.4059789/94‑1 (MQ=60)

                                                                                    AACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCT           >  SRR4302533.1108164/1‑94 (MQ=60)

                                                                                     ACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTT          <  SRR4302533.2944062/94‑1 (MQ=60)

                                                                                     ACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTT          >  SRR4302533.5186685/1‑94 (MQ=60)

                                                                                     ACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTT          >  SRR4302533.2713602/1‑94 (MQ=60)

                                                                                     ACATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTT          >  SRR4302533.4533050/1‑94 (MQ=60)

                                                                                     ACATCCGCGCTTCACAATCGACATGGCCCGTGCAGACAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTT          >  SRR4302533.5186686/1‑94 (MQ=60)

                                                                                      CATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTT         <  SRR4302533.2758009/94‑1 (MQ=60)

                                                                                      AATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTT         <  SRR4302533.4594474/94‑1 (MQ=60)

                                                                                       ATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTA        >  SRR4302533.5272445/1‑94 (MQ=60)

                                                                                       ATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTA        <  SRR4302533.1329814/94‑1 (MQ=60)

                                                                                       ATCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTA        >  SRR4302533.1972121/1‑94 (MQ=60)

                                                                                        TCCGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCACAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAA       >  SRR4302533.2613107/1‑94 (MQ=60)

                                                                                          CGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAA     >  SRR4302533.6296748/1‑94 (MQ=60)

                                                                                          CGCGCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAA     >  SRR4302533.5544421/1‑94 (MQ=60)

                                                                                             GCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTTCTTTAAAAAAG  >  SRR4302533.1315250/1‑94 (MQ=60)

                                                                                             GCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  <  SRR4302533.1461176/94‑1 (MQ=60)

                                                                                             GCTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  >  SRR4302533.544929/1‑94 (MQ=60)

CACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAG  >  NC_000913/4476526‑4476712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`