Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,068 +C intergenic (‑178/‑242) b4256 ← / → yjgN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,0681.C99.5% 621.2 / ‑4.0 192intergenic (‑178/‑242)b4256/yjgNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (44/147);  total (44/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 203 total aligned reads displayed.

GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT  >  NC_000913/4476975‑4477154
                                                                                               |                                                                                      
ggTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑g                                                                                         <  1:4250748/93‑1 (MQ=255)
 gtgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gc                                                                                        >  1:3776881/1‑92 (MQ=255)
  tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gca                                                                                       >  1:152797/1‑91 (MQ=255)
    tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gcatt                                                                                     >  2:2900490/1‑89 (MQ=255)
      gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTAc                                                                                   >  1:1580455/1‑93 (MQ=255)
       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa                                                                                  >  2:3239100/1‑93 (MQ=255)
       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa                                                                                  <  1:5114762/93‑1 (MQ=255)
       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa                                                                                  <  2:2575818/93‑1 (MQ=255)
       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACa                                                                                  >  2:2792370/1‑93 (MQ=255)
        cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg                                                                                 >  2:2432201/1‑93 (MQ=255)
        cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg                                                                                 <  2:4157878/93‑1 (MQ=255)
        cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg                                                                                 >  2:188473/1‑93 (MQ=255)
         tttATCAGCGGTGAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAgg                                                                                <  2:5301336/93‑1 (MQ=255)
         tttATCAGAGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGTATTATCCTGCAGCGTCAGGCGAC‑GCATTACAgg                                                                                <  1:413970/93‑1 (MQ=255)
          ttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGt                                                                               <  2:3089735/93‑1 (MQ=255)
            aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCTGGCGAC‑GCATTACAGGTGa                                                                             <  2:6159145/93‑1 (MQ=255)
            aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGa                                                                             >  2:6292715/1‑93 (MQ=255)
            aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGa                                                                             <  1:31865/93‑1 (MQ=255)
            aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGa                                                                             <  1:703647/93‑1 (MQ=255)
             tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAt                                                                            <  2:4436400/93‑1 (MQ=255)
              cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAtt                                                                           <  1:2059320/93‑1 (MQ=255)
                gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc                                                                         <  2:2986572/93‑1 (MQ=255)
                gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc                                                                         <  2:3054321/93‑1 (MQ=255)
                 cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcg                                                                        <  2:3731005/93‑1 (MQ=255)
                  ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcgg                                                                       <  2:5757658/93‑1 (MQ=255)
                   gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgcggg                                                                      >  2:5606024/1‑92 (MQ=255)
                       gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa                                                                  >  1:2157042/1‑93 (MQ=255)
                       gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa                                                                  <  2:899533/93‑1 (MQ=255)
                       gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa                                                                  >  2:1310206/1‑93 (MQ=255)
                       gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa                                                                  <  2:5951397/93‑1 (MQ=255)
                       aaCCGTATTCGGCGGATACCTGACGAATCACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGACTGCGGCGCaa                                                                  <  2:1118274/92‑1 (MQ=255)
                        aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt                                                                 <  1:5047627/93‑1 (MQ=255)
                         ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATg                                                                <  1:5493575/93‑1 (MQ=255)
                          cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGt                                                               >  1:1717395/1‑93 (MQ=255)
                          cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGt                                                               >  1:2104947/1‑93 (MQ=255)
                          cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGt                                                               >  1:5750268/1‑93 (MQ=255)
                            tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTa                                                             <  2:2144539/93‑1 (MQ=255)
                            tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTa                                                             <  2:608636/93‑1 (MQ=255)
                            tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTa                                                             <  2:1624233/93‑1 (MQ=255)
                             aTTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAt                                                            <  1:1907114/93‑1 (MQ=255)
                              ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt                                                           <  1:4762182/93‑1 (MQ=255)
                                cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa                                                         <  1:3483369/93‑1 (MQ=255)
                                cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa                                                         <  1:6009643/93‑1 (MQ=255)
                                 ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAt                                                        <  2:4322078/93‑1 (MQ=255)
                                 ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAt                                                        <  1:3081613/93‑1 (MQ=255)
                                  gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                       <  1:4413784/93‑1 (MQ=255)
                                  gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                       >  1:2571866/1‑93 (MQ=255)
                                    ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                     <  1:3722131/93‑1 (MQ=255)
                                       tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                                                  >  1:3897640/1‑93 (MQ=255)
                                       tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                                                  <  1:4966460/93‑1 (MQ=255)
                                        aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa                                                 <  2:3925070/93‑1 (MQ=255)
                                        aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa                                                 <  1:4325991/93‑1 (MQ=255)
                                          cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc                                               >  1:465465/1‑93 (MQ=255)
                                           tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                              <  1:5697839/93‑1 (MQ=255)
                                           tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                              >  2:1721213/1‑93 (MQ=255)
                                            gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCa                                             >  2:4494519/1‑93 (MQ=255)
                                             aCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaa                                            <  2:5160311/93‑1 (MQ=255)
                                              cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                           <  2:2202394/93‑1 (MQ=255)
                                               gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata                                          <  1:3269176/93‑1 (MQ=255)
                                                aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa                                         >  1:482732/1‑93 (MQ=255)
                                                aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa                                         >  1:3950003/1‑93 (MQ=255)
                                                 aTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaa                                        <  1:5362864/93‑1 (MQ=255)
                                                  tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa                                       <  2:4078352/93‑1 (MQ=255)
                                                   gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaa                                      <  1:6254042/93‑1 (MQ=255)
                                                     cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaata                                    <  2:5633335/93‑1 (MQ=255)
                                                       cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataaa                                  <  2:4831810/93‑1 (MQ=255)
                                                        gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                 >  2:1857359/1‑93 (MQ=255)
                                                        gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                 >  1:1983465/1‑93 (MQ=255)
                                                        gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                 <  2:4528107/93‑1 (MQ=255)
                                                         ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt                                <  2:795837/93‑1 (MQ=255)
                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACTC‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                               <  2:4702995/93‑1 (MQ=255)
                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                               <  1:5590860/93‑1 (MQ=255)
                                                            gATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt                             <  1:6238869/93‑1 (MQ=255)
                                                               tagcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                           <  2:5064800/89‑1 (MQ=255)
                                                               tagcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                           <  1:973781/89‑1 (MQ=255)
                                                                agcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtat                          <  1:1173210/90‑1 (MQ=255)
                                                                 gcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                         >  1:263734/3‑93 (MQ=255)
                                                                 gcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                         <  1:2653444/91‑1 (MQ=255)
                                                                  caGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAc                        >  2:2246161/2‑93 (MQ=255)
                                                                  caGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAc                        <  1:5248079/92‑1 (MQ=255)
                                                                   aGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                       <  1:3369392/93‑1 (MQ=255)
                                                                   aGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                       <  2:2068974/93‑1 (MQ=255)
                                                                   aGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                       <  2:4600850/93‑1 (MQ=255)
                                                                    ggATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                      <  1:828594/93‑1 (MQ=255)
                                                                     gATTATCGTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt                     <  1:3028591/93‑1 (MQ=255)
                                                                     gATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt                     <  2:3696536/93‑1 (MQ=255)
                                                                     gATAATCCGGCAGCTTCAGGCGAC‑GCATAACAGGTGATTGCGGCGCAAGGTAATTCATATTTAAACCCAAAAAAAATAAAGTAATTATACAtt                     <  2:3945661/93‑1 (MQ=255)
                                                                        tATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGt                  <  2:1535616/93‑1 (MQ=255)
                                                                         aTCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGtt                 >  1:534630/1‑93 (MQ=255)
                                                                           ccTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTa               <  2:4126394/93‑1 (MQ=255)
                                                                            cTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTaa              >  2:1935712/1‑93 (MQ=255)
                                                                             tGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAt             <  2:3491053/93‑1 (MQ=255)
                                                                               cAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAc           <  1:6290870/93‑1 (MQ=255)
                                                                                  cGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc        >  2:4408169/1‑93 (MQ=255)
                                                                                  cGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc        <  1:3869159/93‑1 (MQ=255)
                                                                                  cGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc        <  1:2267678/93‑1 (MQ=255)
                                                                                   gTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAct       >  2:2123231/1‑93 (MQ=255)
                                                                                    tCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActc      <  1:1442650/93‑1 (MQ=255)
                                                                                       ggCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctct   <  1:106575/93‑1 (MQ=255)
                                                                                       ggCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctct   <  1:3631949/93‑1 (MQ=255)
                                                                                               |                                                                                      
GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT  >  NC_000913/4476975‑4477154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT  >  NC_000913/4476975‑4477157
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GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG‑C                                                                                          >  SRR4302533.3800028/1‑94 (MQ=60)
GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG‑C                                                                                          <  SRR4302533.4276394/94‑1 (MQ=60)
 GTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGca                                                                                          >  SRR4302533.153773/1‑92 (MQ=60)
   GTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATT                                                                                        >  SRR4302533.4810539/1‑94 (MQ=60)
     AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAC                                                                                      <  SRR4302533.3349567/94‑1 (MQ=60)
     AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAC                                                                                      >  SRR4302533.1589973/1‑94 (MQ=60)
     AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAC                                                                                      <  SRR4302533.2762463/94‑1 (MQ=60)
       CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                                                    <  SRR4302533.5145672/94‑1 (MQ=60)
       CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                                                    <  SRR4302533.2269277/94‑1 (MQ=60)
       CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                                                    <  SRR4302533.3061292/94‑1 (MQ=60)
        CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                                                   <  SRR4302533.2951335/94‑1 (MQ=60)
         TTTATCAGAGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGTATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                                                  <  SRR4302533.416453/94‑1 (MQ=60)
          TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTG                                                                                 <  SRR4302533.3734280/94‑1 (MQ=60)
          TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTG                                                                                 <  SRR4302533.5060072/94‑1 (MQ=60)
            ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAT                                                                               <  SRR4302533.32085/94‑1 (MQ=60)
            ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAT                                                                               <  SRR4302533.707807/94‑1 (MQ=60)
              CAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTG                                                                             <  SRR4302533.2072018/94‑1 (MQ=60)
               AGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGC                                                                            >  SRR4302533.1377219/1‑94 (MQ=60)
                GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                                           <  SRR4302533.3472846/94‑1 (MQ=60)
                  GGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGC                                                                         <  SRR4302533.5173081/94‑1 (MQ=60)
                   GTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCG                                                                        >  SRR4302533.3223587/1‑94 (MQ=60)
                      AGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAA                                                                     >  SRR4302533.2170322/1‑94 (MQ=60)
                        ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                                                   <  SRR4302533.5078159/94‑1 (MQ=60)
                         CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                  <  SRR4302533.1088421/94‑1 (MQ=60)
                         CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                  >  SRR4302533.2117927/1‑94 (MQ=60)
                         CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                  >  SRR4302533.683182/1‑94 (MQ=60)
                         CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                  <  SRR4302533.5526713/94‑1 (MQ=60)
                         CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                  >  SRR4302533.1727805/1‑94 (MQ=60)
                         CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                  >  SRR4302533.5784958/1‑94 (MQ=60)
                             ATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATT                                                              >  SRR4302533.947744/1‑94 (MQ=60)
                             ATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATT                                                              <  SRR4302533.2026791/94‑1 (MQ=60)
                             ATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATT                                                              <  SRR4302533.1918746/94‑1 (MQ=60)
                              TTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTC                                                             <  SRR4302533.4791067/94‑1 (MQ=60)
                               TCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCA                                                            <  SRR4302533.254407/94‑1 (MQ=60)
                                CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                           <  SRR4302533.6046052/94‑1 (MQ=60)
                                CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                           >  SRR4302533.3355122/1‑94 (MQ=60)
                                CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                           <  SRR4302533.3504856/94‑1 (MQ=60)
                                 GGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATA                                                          <  SRR4302533.3100520/94‑1 (MQ=60)
                                 GGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATA                                                          >  SRR4302533.2587691/1‑94 (MQ=60)
                                  GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                         <  SRR4302533.4440436/94‑1 (MQ=60)
                                  GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                         <  SRR4302533.818011/94‑1 (MQ=60)
                                   CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCACGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                                                        >  SRR4302533.3798678/1‑94 (MQ=60)
                                    GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                       <  SRR4302533.3744973/94‑1 (MQ=60)
                                     GATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTT                                                      >  SRR4302533.5294300/1‑94 (MQ=60)
                                      ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                     <  SRR4302533.5773057/94‑1 (MQ=60)
                                      ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                     >  SRR4302533.3921422/1‑94 (MQ=60)
                                       TACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAA                                                    <  SRR4302533.4996567/94‑1 (MQ=60)
                                        ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                   <  SRR4302533.4352051/94‑1 (MQ=60)
                                         CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                                                  >  SRR4302533.468243/1‑94 (MQ=60)
                                           TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                                                <  SRR4302533.5732257/94‑1 (MQ=60)
                                              CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                             <  SRR4302533.3103715/94‑1 (MQ=60)
                                               GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAA                                            <  SRR4302533.2631005/94‑1 (MQ=60)
                                               GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAA                                            >  SRR4302533.3974088/1‑94 (MQ=60)
                                               GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAA                                            <  SRR4302533.3289344/94‑1 (MQ=60)
                                               GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAA                                            >  SRR4302533.485614/1‑94 (MQ=60)
                                                 ATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                          >  SRR4302533.1922702/1‑94 (MQ=60)
                                                 ATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                          <  SRR4302533.5395072/94‑1 (MQ=60)
                                                  TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                         <  SRR4302533.4208474/94‑1 (MQ=60)
                                                   GACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAAT                                        <  SRR4302533.3558003/94‑1 (MQ=60)
                                                   GACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAAT                                        <  SRR4302533.6291447/94‑1 (MQ=60)
                                                   GACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAAT                                        <  SRR4302533.1559688/94‑1 (MQ=60)
                                                       CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                    <  SRR4302533.3817370/94‑1 (MQ=60)
                                                       CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                    >  SRR4302533.1995603/1‑94 (MQ=60)
                                                          GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                 <  SRR4302533.5624633/94‑1 (MQ=60)
                                                          GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                 <  SRR4302533.2165777/94‑1 (MQ=60)
                                                          GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                 <  SRR4302533.2098570/94‑1 (MQ=60)
                                                           CGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT                                <  SRR4302533.5516059/94‑1 (MQ=60)
                                                            GATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT                               <  SRR4302533.3926380/94‑1 (MQ=60)
                                                            GATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT                               <  SRR4302533.6276230/94‑1 (MQ=60)
                                                              TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                             >  SRR4302533.631619/1‑94 (MQ=60)
                                                              TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                             <  SRR4302533.979498/94‑1 (MQ=60)
                                                               AGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                            >  SRR4302533.265334/1‑94 (MQ=60)
                                                               AGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                            <  SRR4302533.1180252/94‑1 (MQ=60)
                                                               AGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                            <  SRR4302533.4288279/94‑1 (MQ=60)
                                                               AGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                            <  SRR4302533.5711105/94‑1 (MQ=60)
                                                                 gcAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAC                           <  SRR4302533.2669744/92‑1 (MQ=60)
                                                                 gcAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAC                           <  SRR4302533.5585098/92‑1 (MQ=60)
                                                                 gcAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAC                           <  SRR4302533.4420563/92‑1 (MQ=60)
                                                                 gcAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAC                           <  SRR4302533.4126341/92‑1 (MQ=60)
                                                                  CAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACA                          <  SRR4302533.5279628/94‑1 (MQ=60)
                                                                   AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                         <  SRR4302533.4721150/94‑1 (MQ=60)
                                                                   AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                         <  SRR4302533.3390202/94‑1 (MQ=60)
                                                                   AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                         <  SRR4302533.4950479/94‑1 (MQ=60)
                                                                    GGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                        <  SRR4302533.833453/94‑1 (MQ=60)
                                                                    GGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                        <  SRR4302533.5385449/94‑1 (MQ=60)
                                                                     GATTATCGTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                       <  SRR4302533.3047124/94‑1 (MQ=60)
                                                                       TTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                     <  SRR4302533.6240107/94‑1 (MQ=60)
                                                                       TTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                     <  SRR4302533.5834279/94‑1 (MQ=60)
                                                                        TATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                    >  SRR4302533.537809/1‑94 (MQ=60)
                                                                          TCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTA                  <  SRR4302533.1517285/94‑1 (MQ=60)
                                                                           CCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAA                 >  SRR4302533.5086624/1‑94 (MQ=60)
                                                                            CTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAT                <  SRR4302533.4873937/94‑1 (MQ=60)
                                                                              GCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAC              <  SRR4302533.265360/94‑1 (MQ=60)
                                                                               CAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACC             <  SRR4302533.1771503/94‑1 (MQ=60)
                                                                               CAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACC             <  SRR4302533.6328384/94‑1 (MQ=60)
                                                                                  CGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT          <  SRR4302533.3892781/94‑1 (MQ=60)
                                                                                  CGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT          <  SRR4302533.4488562/94‑1 (MQ=60)
                                                                                  CGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT          <  SRR4302533.2281704/94‑1 (MQ=60)
                                                                                   GTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACACAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTC         >  SRR4302533.3917260/1‑94 (MQ=60)
                                                                                    TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT        <  SRR4302533.1707477/94‑1 (MQ=60)
                                                                                    TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT        <  SRR4302533.1451352/94‑1 (MQ=60)
                                                                                       GGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT     <  SRR4302533.3654293/94‑1 (MQ=60)
                                                                                       GGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT     <  SRR4302533.6288203/94‑1 (MQ=60)
                                                                                       GGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT     <  SRR4302533.107243/94‑1 (MQ=60)
                                                                                          GACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT  >  SRR4302533.576856/1‑94 (MQ=60)
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GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT  >  NC_000913/4476975‑4477157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑