Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,321 Δ1 bp coding (12/1182 nt) yjgN → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,3210A.100.0% 827.6 / NA 178coding (12/1182 nt)yjgNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (118/60);  total (118/60)

Only 100 of 179 total aligned reads displayed.

AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408
                                                                                         |                                                                                       
aaCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTcc                                                                                    >  2:3745962/1‑93 (MQ=255)
aaCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTcc                                                                                    >  1:4549399/1‑93 (MQ=255)
 aCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCg                                                                                   >  1:5457108/1‑93 (MQ=255)
   aTTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTc                                                                                 >  2:1433781/1‑93 (MQ=255)
   aTTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTc                                                                                 <  2:627929/93‑1 (MQ=255)
   aTTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTc                                                                                 >  1:4465489/1‑93 (MQ=255)
     tttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTccc                                                                               <  2:1911042/93‑1 (MQ=255)
      ttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCa                                                                              >  2:1124831/1‑93 (MQ=255)
        aCTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAtt                                                                            <  1:764249/93‑1 (MQ=255)
        aCTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAtt                                                                            <  2:4418293/93‑1 (MQ=255)
          tgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCg                                                                          >  2:1975527/1‑93 (MQ=255)
          tgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCg                                                                          >  2:1421629/1‑93 (MQ=255)
            tctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtt                                                                        >  1:196221/1‑93 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      <  2:534630/93‑1 (MQ=255)
               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  1:3499954/1‑93 (MQ=255)
                tatatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgtt                                                                    >  2:638511/1‑93 (MQ=255)
                tatatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgtt                                                                    >  2:3869213/1‑93 (MQ=255)
                tatatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgtt                                                                    <  2:3129190/93‑1 (MQ=255)
                 atatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgttt                                                                   >  1:5930015/1‑93 (MQ=255)
                 atatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgttt                                                                   >  2:3878164/1‑93 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  >  2:4112853/1‑93 (MQ=255)
                   atataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGttttt                                                                 <  2:2873461/93‑1 (MQ=255)
                   atataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGttttt                                                                 >  1:6166834/1‑93 (MQ=255)
                     ataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCa                                                               >  1:2851401/1‑93 (MQ=255)
                      taAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              >  1:3679328/1‑93 (MQ=255)
                       aaGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATg                                                             <  1:2397986/93‑1 (MQ=255)
                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           <  1:4382811/93‑1 (MQ=255)
                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          >  1:5348009/1‑93 (MQ=255)
                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          >  2:5598339/1‑93 (MQ=255)
                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          <  1:2153196/93‑1 (MQ=255)
                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          >  1:3626147/1‑93 (MQ=255)
                            atatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACa                                                        >  2:4476720/1‑93 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       >  1:3057073/1‑93 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       >  2:5841242/1‑93 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       <  2:2662203/93‑1 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       <  1:808219/93‑1 (MQ=255)
                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     >  2:866973/1‑93 (MQ=255)
                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     >  1:1011376/1‑93 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  1:955837/93‑1 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:1582299/93‑1 (MQ=255)
                                  aTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgag                                                  <  1:3420682/93‑1 (MQ=255)
                                  aTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgag                                                  <  2:2409093/93‑1 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 >  1:1644070/1‑93 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 >  2:763479/1‑93 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 <  1:1723481/93‑1 (MQ=255)
                                    ggATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagag                                                >  1:5087943/1‑93 (MQ=255)
                                      aTTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGat                                              >  1:3851576/1‑93 (MQ=255)
                                       ttttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGata                                             >  1:3409359/1‑93 (MQ=255)
                                         ttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtt                                           >  2:2173263/1‑93 (MQ=255)
                                         ttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtt                                           >  1:3262774/1‑93 (MQ=255)
                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         >  1:4807510/5‑93 (MQ=255)
                                              aattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCt                                       >  1:1778952/3‑93 (MQ=255)
                                              aattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCt                                       >  1:3571234/3‑93 (MQ=255)
                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCttt                                     >  1:5259989/1‑92 (MQ=255)
                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTa                                     <  2:1302042/93‑1 (MQ=255)
                                                   aGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAttt                                  <  2:3328248/93‑1 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 <  1:2010543/93‑1 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  1:5874880/1‑93 (MQ=255)
                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtg                               >  2:1984526/1‑93 (MQ=255)
                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtg                               >  1:655048/1‑93 (MQ=255)
                                                       aaTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgt                              <  2:4128552/93‑1 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             <  1:794743/93‑1 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             <  1:158890/93‑1 (MQ=255)
                                                         tttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtgg                            >  2:2348167/1‑93 (MQ=255)
                                                          ttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtggt                           <  1:3125698/93‑1 (MQ=255)
                                                          ttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtggt                           >  1:4841228/1‑93 (MQ=255)
                                                           tttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtggtg                          >  1:6291871/1‑93 (MQ=255)
                                                           tttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtggtg                          >  1:6125232/1‑93 (MQ=255)
                                                             tATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGaa                        <  2:2041187/93‑1 (MQ=255)
                                                             tATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGGGAGAGATATTTTCTTATGTGTGTGGTGaa                        >  1:2713544/1‑93 (MQ=255)
                                                               tGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtg                      >  2:4873682/1‑93 (MQ=255)
                                                                ggCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     >  2:128550/1‑93 (MQ=255)
                                                                ggCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     >  2:4787512/1‑93 (MQ=255)
                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    >  2:2949223/1‑93 (MQ=255)
                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    >  2:2430074/1‑93 (MQ=255)
                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   <  2:3460231/93‑1 (MQ=255)
                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   <  1:2992249/93‑1 (MQ=255)
                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   >  1:4100736/1‑93 (MQ=255)
                                                                   tCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgtt                  >  1:5504324/1‑93 (MQ=255)
                                                                   tCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgtt                  >  1:5765358/1‑93 (MQ=255)
                                                                    cAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgttg                 <  1:1063833/93‑1 (MQ=255)
                                                                    cAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTCCTTATTTGTGTGGTGAATGtgttg                 <  2:6071137/93‑1 (MQ=255)
                                                                     aaGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgttgt                >  2:393663/1‑93 (MQ=255)
                                                                          attaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACg           >  1:5004881/1‑93 (MQ=255)
                                                                           ttaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGa          >  1:2489179/1‑93 (MQ=255)
                                                                            taATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAt         <  2:4013155/93‑1 (MQ=255)
                                                                            taATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAt         >  1:189192/1‑93 (MQ=255)
                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        <  2:4258456/93‑1 (MQ=255)
                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        <  1:6039828/93‑1 (MQ=255)
                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       >  2:5727889/1‑93 (MQ=255)
                                                                               tGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtat      >  2:5630910/1‑93 (MQ=255)
                                                                               tGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtat      <  1:1261226/93‑1 (MQ=255)
                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     >  2:5142478/1‑93 (MQ=255)
                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     <  2:117716/93‑1 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    >  1:5529875/1‑93 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    <  2:6116419/93‑1 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    <  2:2571866/93‑1 (MQ=255)
                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  1:3953934/1‑93 (MQ=255)
                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  2:5992577/1‑93 (MQ=255)
                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  1:3532053/1‑93 (MQ=255)
                                                                                         |                                                                                       
AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCT  >  NC_000913/4477229‑4477410
                                                                                            |                                                                                         
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                        <  SRR4302533.4302230/94‑1 (MQ=60)
 AGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTC                                                                                       >  SRR4302533.5037709/1‑94 (MQ=60)
 AGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTC                                                                                       >  SRR4302533.6093239/1‑94 (MQ=60)
  GAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCC                                                                                      >  SRR4302533.4576985/1‑94 (MQ=60)
   AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCG                                                                                     >  SRR4302533.5489986/1‑94 (MQ=60)
     CATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTC                                                                                   >  SRR4302533.4492498/1‑94 (MQ=60)
           ACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTC                                                                             <  SRR4302533.768752/94‑1 (MQ=60)
            CTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCG                                                                            >  SRR4302533.4845171/1‑94 (MQ=60)
              GTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTT                                                                          >  SRR4302533.197460/1‑94 (MQ=60)
                CTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTG                                                                        >  SRR4302533.3657653/1‑94 (MQ=60)
                CTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTG                                                                        >  SRR4302533.2833620/1‑94 (MQ=60)
                 TGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGT                                                                       >  SRR4302533.3521541/1‑94 (MQ=60)
                 TGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGT                                                                       >  SRR4302533.3521540/1‑94 (MQ=60)
                 TGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGT                                                                       <  SRR4302533.5871915/94‑1 (MQ=60)
                  GTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTT                                                                      >  SRR4302533.3502664/1‑94 (MQ=60)
                   TATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTT                                                                     >  SRR4302533.5965865/1‑94 (MQ=60)
                     TATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTT                                                                   >  SRR4302533.6203896/1‑94 (MQ=60)
                     TATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTT                                                                   <  SRR4302533.2447231/94‑1 (MQ=60)
                      ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                  >  SRR4302533.5056667/1‑94 (MQ=60)
                      ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                  <  SRR4302533.3341975/94‑1 (MQ=60)
                      ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                  >  SRR4302533.806245/1‑94 (MQ=60)
                       TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                 >  SRR4302533.2868944/1‑94 (MQ=60)
                       TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                 >  SRR4302533.1995673/1‑94 (MQ=60)
                        ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                >  SRR4302533.3701944/1‑94 (MQ=60)
                          AAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGG                                                              <  SRR4302533.2412788/94‑1 (MQ=60)
                          AAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGG                                                              <  SRR4302533.4434789/94‑1 (MQ=60)
                            GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                            <  SRR4302533.4409271/94‑1 (MQ=60)
                            GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                            >  SRR4302533.3648458/1‑94 (MQ=60)
                            GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                            >  SRR4302533.5380092/1‑94 (MQ=60)
                             TTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAC                                                           <  SRR4302533.2166459/94‑1 (MQ=60)
                               ATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAG                                                         >  SRR4302533.3075826/1‑94 (MQ=60)
                                TATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGG                                                        <  SRR4302533.812969/94‑1 (MQ=60)
                                 ATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGT                                                       >  SRR4302533.1939110/1‑94 (MQ=60)
                                 ATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGT                                                       >  SRR4302533.1017295/1‑94 (MQ=60)
                                   CAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGA                                                     >  SRR4302533.6169653/1‑94 (MQ=60)
                                   CAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGA                                                     <  SRR4302533.395380/94‑1 (MQ=60)
                                    AATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAG                                                    >  SRR4302533.5383549/1‑94 (MQ=60)
                                    AATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAG                                                    <  SRR4302533.961450/94‑1 (MQ=60)
                                     ATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGA                                                   <  SRR4302533.3441818/94‑1 (MQ=60)
                                     ATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGA                                                   >  SRR4302533.1118825/1‑94 (MQ=60)
                                     ATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGA                                                   >  SRR4302533.3011273/1‑94 (MQ=60)
                                     ATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGA                                                   >  SRR4302533.1654024/1‑94 (MQ=60)
                                      TGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAG                                                  <  SRR4302533.1733935/94‑1 (MQ=60)
                                      TGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAG                                                  >  SRR4302533.5118686/1‑94 (MQ=60)
                                        GATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGAT                                                >  SRR4302533.3875097/1‑94 (MQ=60)
                                         ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                               <  SRR4302533.784835/94‑1 (MQ=60)
                                         ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                               >  SRR4302533.3430418/1‑94 (MQ=60)
                                         ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                               <  SRR4302533.6079488/94‑1 (MQ=60)
                                           TTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATT                                             >  SRR4302533.3282903/1‑94 (MQ=60)
                                             TTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTT                                           >  SRR4302533.4836617/1‑94 (MQ=60)
                                              TTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTC                                          >  SRR4302533.2550482/1‑94 (MQ=60)
                                               TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                         >  SRR4302533.3593242/1‑94 (MQ=60)
                                               TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                         >  SRR4302533.1789783/1‑94 (MQ=60)
                                               TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCAATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                         <  SRR4302533.921486/94‑1 (MQ=60)
                                                  ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTT                                       >  SRR4302533.5291569/1‑94 (MQ=60)
                                                   TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAT                                      >  SRR4302533.321256/1‑94 (MQ=60)
                                                   TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAT                                      <  SRR4302533.5632509/94‑1 (MQ=60)
                                                    TCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATT                                     <  SRR4302533.4021748/94‑1 (MQ=60)
                                                    TCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATT                                     >  SRR4302533.1752766/1‑94 (MQ=60)
                                                     CAGAGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTT                                    >  SRR4302533.5562184/1‑94 (MQ=60)
                                                      AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                   >  SRR4302533.599282/1‑94 (MQ=60)
                                                      AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                   >  SRR4302533.5910392/1‑94 (MQ=60)
                                                       GGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGT                                  <  SRR4302533.2022929/94‑1 (MQ=60)
                                                        GGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTG                                 >  SRR4302533.658915/1‑94 (MQ=60)
                                                          AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                               <  SRR4302533.3732163/94‑1 (MQ=60)
                                                          AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                               <  SRR4302533.1868686/94‑1 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                              <  SRR4302533.799418/94‑1 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                              <  SRR4302533.159902/94‑1 (MQ=60)
                                                            TTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGT                             >  SRR4302533.4870571/1‑94 (MQ=60)
                                                             TTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTG                            >  SRR4302533.161299/1‑94 (MQ=60)
                                                             TTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTG                            >  SRR4302533.6329387/1‑94 (MQ=60)
                                                             TTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTG                            <  SRR4302533.3144906/94‑1 (MQ=60)
                                                             TTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTG                            >  SRR4302533.6162151/1‑94 (MQ=60)
                                                               TTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGGGAGAGATATTTTCTTATGTGTGTGGTGAA                          >  SRR4302533.2730207/1‑94 (MQ=60)
                                                                    GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                     >  SRR4302533.4125692/1‑94 (MQ=60)
                                                                     CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                    >  SRR4302533.462593/1‑94 (MQ=60)
                                                                     CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                    >  SRR4302533.5800149/1‑94 (MQ=60)
                                                                     CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                    <  SRR4302533.3010544/94‑1 (MQ=60)
                                                                     CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                    >  SRR4302533.5537535/1‑94 (MQ=60)
                                                                       CAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGT                  >  SRR4302533.6227097/1‑94 (MQ=60)
                                                                       CAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGT                  <  SRR4302533.1070083/94‑1 (MQ=60)
                                                                           TTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATCTTCTTATTTGTGTGGTGAATGTGTTGTTAAC              >  SRR4302533.3585931/1‑94 (MQ=60)
                                                                            TATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACG             >  SRR4302533.5035212/1‑94 (MQ=60)
                                                                            TATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACG             >  SRR4302533.5326910/1‑94 (MQ=60)
                                                                             ATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGA            >  SRR4302533.2504508/1‑94 (MQ=60)
                                                                              TTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAT           >  SRR4302533.190371/1‑94 (MQ=60)
                                                                                AATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTA         <  SRR4302533.6076426/94‑1 (MQ=60)
                                                                                  TGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATA       >  SRR4302533.704449/1‑94 (MQ=60)
                                                                                  TGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATA       >  SRR4302533.2404492/1‑94 (MQ=60)
                                                                                  TGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATA       <  SRR4302533.1268768/94‑1 (MQ=60)
                                                                                   GAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAA      >  SRR4302533.5563258/1‑94 (MQ=60)
                                                                                   GAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAA      >  SRR4302533.5228326/1‑94 (MQ=60)
                                                                                    AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC     <  SRR4302533.3121659/94‑1 (MQ=60)
                                                                                    AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC     >  SRR4302533.1515474/1‑94 (MQ=60)
                                                                                     AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG    >  SRR4302533.3553820/1‑94 (MQ=60)
                                                                                     AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG    >  SRR4302533.3978041/1‑94 (MQ=60)
                                                                                     AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG    >  SRR4302533.4527862/1‑94 (MQ=60)
                                                                                       TGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCT  >  SRR4302533.5850906/1‑94 (MQ=60)
                                                                                       TGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAAGATTATAACGCT  <  SRR4302533.408981/94‑1 (MQ=60)
                                                                                            |                                                                                         
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCT  >  NC_000913/4477229‑4477410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑