Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,478,503 (A)7→6 intergenic (+12/+47) yjgN → / ← valS hypothetical protein/valine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,478,4970A.99.5% 929.7 / ‑2.7 202intergenic (+6/+53)yjgN/valShypothetical protein/valine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (111/90);  total (112/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 235 total aligned reads displayed.

CCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT  >  NC_000913/4478405‑4478588
                                                                                            |                                                                                           
 cttTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaa                                                                                            <  1:6043330/93‑1 (MQ=255)
 cttTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaa                                                                                            <  1:3299928/93‑1 (MQ=255)
  tttAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaa                                                                                           <  1:4325325/93‑1 (MQ=255)
   ttAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaa                                                                                          >  1:729953/1‑93 (MQ=255)
    tAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaa                                                                                         >  1:829094/1‑93 (MQ=255)
     aaTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaa                                                                                        >  2:3880655/1‑93 (MQ=255)
      aTCAATAATGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGGAAGAGATaaaaaag                                                                                       >  2:6119289/1‑92 (MQ=255)
      aTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaag                                                                                       <  1:1173049/93‑2 (MQ=255)
      aTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaag                                                                                       <  2:5979009/93‑2 (MQ=255)
      aTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaag                                                                                       <  1:3252536/93‑2 (MQ=255)
       tCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaagg                                                                                      >  2:2270703/1‑91 (MQ=255)
        cAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggc                                                                                     >  2:4588021/1‑90 (MQ=255)
        cAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggc                                                                                     >  1:4559986/1‑90 (MQ=255)
         aaTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggcc                                                                                    >  2:3160187/1‑89 (MQ=255)
         aaTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggcc                                                                                    >  1:2788301/1‑89 (MQ=255)
          aTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCg                                                                                  <  1:1377493/93‑1 (MQ=255)
          aTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCg                                                                                  >  2:4166479/1‑93 (MQ=255)
            aaaGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGa                                                                                <  1:483137/93‑1 (MQ=255)
            aaaGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGa                                                                                <  2:2777150/93‑1 (MQ=255)
              agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagc                                                                              >  1:6304963/1‑91 (MQ=255)
              agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagc                                                                              >  2:2381343/1‑91 (MQ=255)
              agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagc                                                                              <  2:4248560/93‑3 (MQ=255)
                aagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagcat                                                                            <  1:5486190/93‑5 (MQ=255)
                aagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagcat                                                                            >  2:204153/1‑89 (MQ=255)
                 agaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTGCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGga‑gcatg                                                                          <  2:5814335/93‑1 (MQ=255)
                  gaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGga‑gcatgc                                                                         >  2:5638347/1‑93 (MQ=255)
                     caacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTcc                                                                      <  2:4664308/93‑1 (MQ=255)
                      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCg                                                                     >  1:5811288/1‑93 (MQ=255)
                      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCg                                                                     >  1:5733990/1‑93 (MQ=255)
                      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCg                                                                     >  2:4818249/1‑93 (MQ=255)
                      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCg                                                                     >  1:4257011/1‑93 (MQ=255)
                       acCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                    >  2:2941464/1‑93 (MQ=255)
                        ccAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGc                                                                   >  1:6289206/1‑93 (MQ=255)
                         cAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGcc                                                                  <  2:1636209/93‑1 (MQ=255)
                          aGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCt                                                                 <  1:3054490/93‑1 (MQ=255)
                          aGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCt                                                                 >  1:5698412/1‑93 (MQ=255)
                           gATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCtt                                                                <  1:6125481/93‑1 (MQ=255)
                           gATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCtt                                                                >  2:3435890/1‑93 (MQ=255)
                            aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttc                                                               >  2:2438121/1‑92 (MQ=255)
                            aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGAAAGAAAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttc                                                               <  2:4461821/93‑2 (MQ=255)
                             tAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcg                                                              <  1:2530522/93‑3 (MQ=255)
                             tAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcg                                                              >  1:2655208/1‑91 (MQ=255)
                             tAAAGCCTTCTTAGCCAGTATTTCACGGTGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcg                                                              <  1:2399275/93‑3 (MQ=255)
                               aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                            >  2:3093059/1‑89 (MQ=255)
                               aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                            >  2:6248537/1‑89 (MQ=255)
                                aGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGttt                                                          >  1:464717/1‑93 (MQ=255)
                                 ggCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTTTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGtttt                                                         >  2:2628728/1‑93 (MQ=255)
                                  gCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtc                                                        <  1:1475142/93‑1 (MQ=255)
                                    ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcat                                                      >  1:534623/1‑93 (MQ=255)
                                         aGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtg                                                 >  1:3534506/1‑93 (MQ=255)
                                          gCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgt                                                >  1:4971656/1‑93 (MQ=255)
                                            cAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTgtgt                                              >  1:393612/1‑93 (MQ=255)
                                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtt                                             <  2:4952738/93‑1 (MQ=255)
                                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtt                                             >  2:1576657/1‑93 (MQ=255)
                                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtt                                             >  1:1666099/1‑93 (MQ=255)
                                              gTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGGGAT‑AAAAAAGGGCGGA‑GCATGCGCCGGACCT‑CGTTTTCCTCACTctgtct                                            >  1:3384476/1‑91 (MQ=255)
                                               tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttt                                           <  2:1354465/93‑1 (MQ=255)
                                               tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttt                                           >  2:287573/1‑93 (MQ=255)
                                               tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttt                                           <  1:183097/93‑1 (MQ=255)
                                                 tttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttttt                                         >  1:2324666/1‑91 (MQ=255)
                                                 tttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGa                                         >  1:3825391/1‑93 (MQ=255)
                                                  ttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGAt                                        <  1:2996871/93‑1 (MQ=255)
                                                     aCGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTAc                                     <  2:1672795/93‑1 (MQ=255)
                                                       ggggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                   >  2:1646558/1‑93 (MQ=255)
                                                       ggggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                   <  1:943687/93‑1 (MQ=255)
                                                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                  <  2:3549120/93‑1 (MQ=255)
                                                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                  <  2:5130838/93‑1 (MQ=255)
                                                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                  >  2:5113872/1‑93 (MQ=255)
                                                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                  <  1:4367886/93‑1 (MQ=255)
                                                          gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                                >  2:3766652/1‑93 (MQ=255)
                                                          gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                                >  2:1291854/1‑93 (MQ=255)
                                                             ttATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcggc                             <  2:5187577/93‑1 (MQ=255)
                                                             ttATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcggc                             >  1:5943837/1‑93 (MQ=255)
                                                             ttATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGCGTTTTGATTACAGCgcggc                             <  2:5288824/93‑1 (MQ=255)
                                                             ttATGCCTCCTTTGCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcggc                             <  1:5574536/93‑1 (MQ=255)
                                                               aTGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGa                           >  1:1770879/1‑93 (MQ=255)
                                                                 gCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGAGATa                         >  2:3608029/1‑93 (MQ=255)
                                                                 gCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCTT‑CGTTTTTATTCCTGTGTTTTTATTTAAGAGCGGCGCTa                         >  2:5397587/1‑93 (MQ=255)
                                                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATaa                        >  2:4066487/1‑93 (MQ=255)
                                                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATaa                        >  1:3481991/1‑93 (MQ=255)
                                                                   cttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                       <  2:1881536/93‑1 (MQ=255)
                                                                   cttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                       >  1:5191356/1‑93 (MQ=255)
                                                                    ttcttTAACATTTCTGCAAGAGAT‑AAACAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                      <  1:1965405/93‑1 (MQ=255)
                                                                      cttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGc                    <  1:1643051/93‑1 (MQ=255)
                                                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGcc                   <  2:860114/93‑1 (MQ=255)
                                                                          aCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgc                >  1:873452/1‑93 (MQ=255)
                                                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTTCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgct               >  1:216223/1‑93 (MQ=255)
                                                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgct               <  2:4121392/93‑1 (MQ=255)
                                                                             aTTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGt             <  2:2908083/93‑1 (MQ=255)
                                                                              tttCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTTCGGGCTT‑CGTTTTTATCACTGTGTTTTGATTTCCACGCGGGGATAACAGCCTTCTGtt            >  2:214538/1‑93 (MQ=255)
                                                                              tttCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGtt            <  2:5349634/93‑1 (MQ=255)
                                                                               ttCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTc           <  2:2534234/93‑1 (MQ=255)
                                                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc       >  2:115210/1‑93 (MQ=255)
                                                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc       >  1:2899809/1‑93 (MQ=255)
                                                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc       <  2:729798/93‑1 (MQ=255)
                                                                                    tAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCa      <  2:2167961/93‑1 (MQ=255)
                                                                                     aaGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAg     <  2:5520033/93‑1 (MQ=255)
                                                                                      agagAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt    <  2:4912371/93‑1 (MQ=255)
                                                                                      agagAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt    >  1:1024095/1‑93 (MQ=255)
                                                                                       gagaT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtt   <  1:370065/93‑1 (MQ=255)
                                                                                            |                                                                                           
CCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT  >  NC_000913/4478405‑4478588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC  >  NC_000913/4478411‑4478597
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ATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGG                                                                                               <  SRR4302533.3272592/94‑1 (MQ=60)
ATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGG                                                                                               <  SRR4302533.1180089/94‑1 (MQ=60)
 TCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGC                                                                                             <  SRR4302533.3731804/94‑1 (MQ=60)
 TCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGC                                                                                             >  SRR4302533.4587645/1‑94 (MQ=60)
  CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                            >  SRR4302533.2849451/1‑94 (MQ=60)
  CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                            <  SRR4302533.6262532/94‑1 (MQ=60)
  CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                            >  SRR4302533.2805435/1‑94 (MQ=60)
   AATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCG                                                                                           <  SRR4302533.797298/94‑1 (MQ=60)
    ATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG                                                                                          <  SRR4302533.1385779/94‑1 (MQ=60)
     TAAAGAAGAACAACCAGATAAAGCCTTCTGAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA                                                                                         <  SRR4302533.2122555/94‑1 (MQ=60)
      AAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGAG                                                                                        <  SRR4302533.486020/94‑1 (MQ=60)
        AGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCA                                                                                     >  SRR4302533.4861472/1‑94 (MQ=60)
        AGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTTACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGccggggga                                                                                       >  SRR4302533.3563494/1‑86 (MQ=60)
          AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                   <  SRR4302533.5519258/94‑1 (MQ=60)
          AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                   <  SRR4302533.933614/94‑1 (MQ=60)
           AGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGC                                                                                  <  SRR4302533.3172819/94‑1 (MQ=60)
            GAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCT                                                                                 >  SRR4302533.4331950/1‑94 (MQ=60)
              ACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCC                                                                               >  SRR4302533.6342953/1‑94 (MQ=60)
              ACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCC                                                                               <  SRR4302533.3324131/94‑1 (MQ=60)
               CAACCAGATAAAGGTTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                              <  SRR4302533.3590094/94‑1 (MQ=60)
               CAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                              >  SRR4302533.4282676/1‑94 (MQ=60)
               CAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                              >  SRR4302533.5846384/1‑94 (MQ=60)
               CAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                              >  SRR4302533.5768607/1‑94 (MQ=60)
               CAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                              <  SRR4302533.5738891/94‑1 (MQ=60)
                 ACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGC                                                                            >  SRR4302533.6326718/1‑94 (MQ=60)
                 ACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGC                                                                            >  SRR4302533.196817/1‑94 (MQ=60)
                 ACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGC                                                                            >  SRR4302533.4892562/1‑94 (MQ=60)
                  CCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCC                                                                           >  SRR4302533.87632/1‑94 (MQ=60)
                   CAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCT                                                                          >  SRR4302533.5732830/1‑94 (MQ=60)
                    AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                         <  SRR4302533.3073209/94‑1 (MQ=60)
                    AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                         <  SRR4302533.3720834/94‑1 (MQ=60)
                    AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                         >  SRR4302533.4033325/1‑94 (MQ=60)
                    AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG‑GGCATGCTCCGGGCTT                                                                         >  SRR4302533.3445733/1‑94 (MQ=60)
                     GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTC                                                                        <  SRR4302533.6162402/94‑1 (MQ=60)
                      ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                       >  SRR4302533.5741868/1‑92 (MQ=60)
                      ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                       >  SRR4302533.2671516/1‑92 (MQ=60)
                       TAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                     <  SRR4302533.2546101/94‑1 (MQ=60)
                       TAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                     >  SRR4302533.1685924/1‑94 (MQ=60)
                       TAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                     >  SRR4302533.1636129/1‑94 (MQ=60)
                       TAAAGCCTTCTTAGCCAGTATTTCACGGTGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                     <  SRR4302533.2414091/94‑1 (MQ=60)
                        AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGCCTT‑CGTT                                                                    >  SRR4302533.1100517/1‑94 (MQ=60)
                        AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                    >  SRR4302533.1819886/1‑94 (MQ=60)
                         AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGGCCGGgggcgcgcccgccttcgttt                                                                      >  SRR4302533.1132677/1‑74 (MQ=60)
                         AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                   <  SRR4302533.4446949/94‑1 (MQ=60)
                         AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                   <  SRR4302533.6148912/94‑1 (MQ=60)
                         AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                   >  SRR4302533.467492/1‑94 (MQ=60)
                         AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                   >  SRR4302533.4842982/1‑94 (MQ=60)
                            GCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCA                                                                <  SRR4302533.1484058/94‑1 (MQ=60)
                             CTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAT                                                               >  SRR4302533.537802/1‑94 (MQ=60)
                                  TAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTG                                                          >  SRR4302533.3556287/1‑94 (MQ=60)
                                   AGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGT                                                         >  SRR4302533.1389518/1‑94 (MQ=60)
                                   AGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGT                                                         >  SRR4302533.5001800/1‑94 (MQ=60)
                                    GCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG                                                        <  SRR4302533.2980170/94‑1 (MQ=60)
                                     CCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGT                                                       >  SRR4302533.395999/1‑94 (MQ=60)
                                      CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTT                                                      >  SRR4302533.1676167/1‑94 (MQ=60)
                                       AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                     <  SRR4302533.5157280/94‑1 (MQ=60)
                                        GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                    <  SRR4302533.1278866/94‑1 (MQ=60)
                                        GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                    >  SRR4302533.5555426/1‑94 (MQ=60)
                                         TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                   >  SRR4302533.4579078/1‑94 (MQ=60)
                                         TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                   <  SRR4302533.184235/94‑1 (MQ=60)
                                         TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                   <  SRR4302533.6001531/94‑1 (MQ=60)
                                          ATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTtt                                                  >  SRR4302533.2339057/1‑92 (MQ=60)
                                          ATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGA                                                  >  SRR4302533.3848777/1‑94 (MQ=60)
                                            TTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATT                                                >  SRR4302533.2376409/1‑94 (MQ=60)
                                            TTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATT                                                <  SRR4302533.3015196/94‑1 (MQ=60)
                                               ACGGGGAGTTATGCCTTCTTTCCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACA                                             <  SRR4302533.2661910/94‑1 (MQ=60)
                                                 GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGC                                           <  SRR4302533.949235/94‑1 (MQ=60)
                                                 GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGC                                           <  SRR4302533.3393532/94‑1 (MQ=60)
                                                  GGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCG                                          <  SRR4302533.4394253/94‑1 (MQ=60)
                                                   GGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGC                                         >  SRR4302533.5916138/1‑94 (MQ=60)
                                                      GTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGC                                      >  SRR4302533.5979784/1‑94 (MQ=60)
                                                       TTATGCCTCCTTTGCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCG                                     <  SRR4302533.5608224/94‑1 (MQ=60)
                                                        TATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGA                                    >  SRR4302533.1781660/1‑94 (MQ=60)
                                                         ATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGAT                                   <  SRR4302533.791835/94‑1 (MQ=60)
                                                           GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                 >  SRR4302533.3503471/1‑94 (MQ=60)
                                                           GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                 <  SRR4302533.2678739/94‑1 (MQ=60)
                                                            CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                >  SRR4302533.5222675/1‑94 (MQ=60)
                                                            CCTCCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                <  SRR4302533.5460007/94‑1 (MQ=60)
                                                             CTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACA                               >  SRR4302533.2484657/1‑94 (MQ=60)
                                                             CTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACA                               <  SRR4302533.3521107/94‑1 (MQ=60)
                                                              TTCTTTACCATTTCTGTAAGAGAT‑CAAACAGGCCGGA‑GCATGCTCCGGCCCT‑CGGTTTCACCACTGTGTTTTGCTTACAGCGCGGCGATCACAG                              >  SRR4302533.4359558/1‑94 (MQ=29)
                                                              TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAG                              >  SRR4302533.5616712/1‑94 (MQ=60)
                                                              TTCTTTAACATTTCTGCAAGAGAT‑AAACAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAG                              <  SRR4302533.1977412/94‑1 (MQ=60)
                                                               TCTTTACTATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGC                             <  SRR4302533.3129075/94‑1 (MQ=60)
                                                                CTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCC                            <  SRR4302533.1652999/94‑1 (MQ=60)
                                                                  TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGATCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTG                          <  SRR4302533.5840366/94‑1 (MQ=60)
                                                                   TACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGC                         >  SRR4302533.878582/1‑94 (MQ=60)
                                                                    ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTTCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                        >  SRR4302533.217581/1‑94 (MQ=60)
                                                                    ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                        <  SRR4302533.6308447/94‑1 (MQ=60)
                                                                     CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                       <  SRR4302533.3276225/94‑1 (MQ=60)
                                                                      CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                      >  SRR4302533.540710/1‑94 (MQ=60)
                                                                       ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTT                     >  SRR4302533.2108912/1‑94 (MQ=60)
                                                                       ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTT                     >  SRR4302533.5315215/1‑94 (MQ=60)
                                                                       ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTT                     <  SRR4302533.1705317/94‑1 (MQ=60)
                                                                         TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                   >  SRR4302533.4128980/1‑94 (MQ=60)
                                                                          TCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAA                  >  SRR4302533.251229/1‑94 (MQ=60)
                                                                            TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                >  SRR4302533.3032261/1‑94 (MQ=60)
                                                                            TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                >  SRR4302533.2917628/1‑94 (MQ=60)
                                                                              TAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAG              >  SRR4302533.221817/1‑94 (MQ=60)
                                                                               AAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGT             >  SRR4302533.1030116/1‑94 (MQ=60)
                                                                                AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT            >  SRR4302533.2898884/1‑94 (MQ=60)
                                                                                AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT            <  SRR4302533.6331384/94‑1 (MQ=60)
                                                                                AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT            >  SRR4302533.5863367/1‑94 (MQ=60)
                                                                                 GAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT           <  SRR4302533.372300/94‑1 (MQ=60)
                                                                                  AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC          <  SRR4302533.3362876/94‑1 (MQ=60)
                                                                                  AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC          >  SRR4302533.4689005/1‑94 (MQ=60)
                                                                                  AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGAGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC          >  SRR4302533.5653535/1‑94 (MQ=60)
                                                                                   GAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCG         >  SRR4302533.3688501/1‑94 (MQ=60)
                                                                                      TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT       >  SRR4302533.5182205/1‑94 (MQ=60)
                                                                                      TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT       >  SRR4302533.1307070/1‑94 (MQ=60)
                                                                                      TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT       <  SRR4302533.4652005/94‑1 (MQ=60)
                                                                                        AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT     >  SRR4302533.2155386/1‑94 (MQ=60)
                                                                                        AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT     <  SRR4302533.777844/94‑1 (MQ=60)
                                                                                        AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT     <  SRR4302533.2075102/94‑1 (MQ=60)
                                                                                        AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT     <  SRR4302533.4977486/94‑1 (MQ=60)
                                                                                         AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC    <  SRR4302533.1953928/94‑1 (MQ=60)
                                                                                         AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC    <  SRR4302533.6230131/94‑1 (MQ=60)
                                                                                         AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC    >  SRR4302533.2060950/1‑94 (MQ=60)
                                                                                         AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC    >  SRR4302533.3380993/1‑94 (MQ=60)
                                                                                         AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC    >  SRR4302533.3204850/1‑94 (MQ=60)
                                                                                          AAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCG   <  SRR4302533.5279702/94‑1 (MQ=60)
                                                                                           AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC  >  SRR4302533.320815/1‑94 (MQ=60)
                                                                                           AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC  <  SRR4302533.2131163/94‑1 (MQ=60)
                                                                                            |                                                                                              
ATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC  >  NC_000913/4478411‑4478597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑