Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,604,832 A→G R146R (CGT→CGC)  yjjT ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,8320AG99.6% 761.7 / ‑4.9 238R146R (CGT→CGC) yjjThypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (124/113);  total (125/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 249 total aligned reads displayed.

AAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC  >  NC_000913/4604742‑4604921
                                                                                          |                                                                                         
  acacGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAggcgac                                                                                       <  2:5198112/93‑1 (MQ=255)
  acacGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAgacgac                                                                                       <  1:1580589/93‑1 (MQ=255)
   cacGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGAcc                                                                                      <  1:1053220/93‑1 (MQ=255)
   cacGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGAcc                                                                                      <  2:3613326/93‑1 (MQ=255)
      gCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCaaa                                                                                   <  2:3204713/93‑1 (MQ=255)
          ggCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATag                                                                               <  1:1920148/93‑1 (MQ=255)
          ggCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATag                                                                               >  1:4140908/1‑93 (MQ=255)
            cAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACTAAAATagag                                                                             <  2:4423248/93‑1 (MQ=255)
            cAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATagag                                                                             <  2:2589064/93‑1 (MQ=255)
            cAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATagag                                                                             <  2:5317559/93‑1 (MQ=255)
            cAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATagag                                                                             <  2:546303/93‑1 (MQ=255)
             tgCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAgg                                                                            <  1:14182/92‑1 (MQ=255)
             aGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAgg                                                                            >  1:5209516/1‑93 (MQ=255)
             aGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAgg                                                                            >  1:2169390/1‑93 (MQ=255)
              gCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCACATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGc                                                                           >  1:285545/1‑93 (MQ=255)
              gCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGc                                                                           <  1:2394755/93‑1 (MQ=255)
              gCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGc                                                                           <  2:4524257/93‑1 (MQ=255)
              gCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGc                                                                           <  1:2465974/93‑1 (MQ=255)
                gTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCca                                                                         >  1:2615902/1‑93 (MQ=255)
                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        >  1:2216547/1‑93 (MQ=255)
                     gACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcg                                                                    >  1:3883860/1‑93 (MQ=255)
                     gACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGACACAGcg                                                                    >  2:4582939/1‑93 (MQ=255)
                      aCCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcga                                                                   <  2:5863676/93‑1 (MQ=255)
                      aCCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcga                                                                   <  1:1195273/93‑1 (MQ=255)
                       ccGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcgac                                                                  <  2:851899/93‑1 (MQ=255)
                         gTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcgacga                                                                <  2:2062533/93‑1 (MQ=255)
                          tCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAg                                                               <  1:5071838/93‑1 (MQ=255)
                             ggCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAgcgc                                                            <  2:3026508/93‑1 (MQ=255)
                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGt                                                         <  1:5848811/93‑1 (MQ=255)
                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGt                                                         <  1:893738/93‑1 (MQ=255)
                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGt                                                         <  2:1611192/93‑1 (MQ=255)
                                 aTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTc                                                        >  2:4161785/1‑93 (MQ=255)
                                 aTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTc                                                        >  2:4000803/1‑93 (MQ=255)
                                  tCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGATGCCACAGCGACGAGCGCTGTCg                                                       >  2:3648934/1‑93 (MQ=255)
                                  tCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCg                                                       >  1:3086469/1‑93 (MQ=255)
                                   cGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCgc                                                      >  2:1918219/1‑92 (MQ=255)
                                   cGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGa                                                      <  2:1685302/93‑1 (MQ=255)
                                     aCGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACt                                                    <  1:2161964/93‑1 (MQ=255)
                                      cGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACtt                                                   >  1:1260846/1‑93 (MQ=255)
                                        cTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTa                                                 <  1:4408117/93‑1 (MQ=255)
                                        cTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAACAGAGGCCACAGCGACGAGCGCTGTCGACtttt                                                 >  2:1830486/1‑92 (MQ=255)
                                         tGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAt                                                >  1:2089540/1‑93 (MQ=255)
                                         tGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAt                                                <  2:135863/93‑1 (MQ=255)
                                          gTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAtt                                               >  1:3297503/1‑93 (MQ=255)
                                          gTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAtt                                               <  2:5353467/93‑1 (MQ=255)
                                           tATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTc                                              <  2:4102911/93‑1 (MQ=255)
                                           tATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTc                                              <  1:4225546/93‑1 (MQ=255)
                                            aTTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCa                                             <  2:5266940/93‑1 (MQ=255)
                                             ttCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCaa                                            >  1:4791854/1‑93 (MQ=255)
                                             tccGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCaa                                            <  2:3098536/91‑1 (MQ=255)
                                              tCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAAc                                           <  1:4066626/93‑1 (MQ=255)
                                              tCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAAc                                           <  1:297688/93‑1 (MQ=255)
                                                gCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACgg                                         <  2:5664677/93‑1 (MQ=255)
                                                 ccccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCCACGgc                                        >  2:140183/1‑93 (MQ=255)
                                                 ccccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgc                                        >  1:1973717/1‑93 (MQ=255)
                                                   ccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgcgc                                      >  2:5116537/1‑93 (MQ=255)
                                                   ccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgcgc                                      >  2:4737789/1‑93 (MQ=255)
                                                   ccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgcgc                                      >  1:1082269/1‑93 (MQ=255)
                                                    cAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCa                                     >  2:6077232/1‑93 (MQ=255)
                                                     aGAATTTCTCCGCATCAAATACCGGCTGTTTTTTCAGGCGACCAAAATAGAGGCCAAAGCGACGAGCGCTGTCGACTTTATTCAACAGCGCAt                                    >  2:3543429/1‑93 (MQ=255)
                                                     aGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCAt                                    >  2:176109/1‑93 (MQ=255)
                                                      gAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATa                                   <  2:1108676/93‑1 (MQ=255)
                                                      gAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATa                                   <  1:4648729/93‑1 (MQ=255)
                                                      gAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATa                                   <  1:3532331/93‑1 (MQ=255)
                                                       aaTTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATaa                                  >  1:2438472/1‑93 (MQ=255)
                                                        aTTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAt                                 <  1:1021429/93‑1 (MQ=255)
                                                        aTTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAt                                 <  1:6012882/93‑1 (MQ=255)
                                                        aTTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAt                                 >  1:5570738/1‑93 (MQ=255)
                                                        aTTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAt                                 <  1:4871849/93‑1 (MQ=255)
                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                >  2:1137778/1‑93 (MQ=255)
                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                <  2:216659/93‑1 (MQ=255)
                                                             tcCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGACACAGCGACGAGCAGCGTCGACTTTATTCAACGGCGTATACTATGcc                            >  2:1211639/1‑93 (MQ=255)
                                                              ccGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCa                           <  2:904718/93‑1 (MQ=255)
                                                              ccGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCa                           >  1:5805866/1‑93 (MQ=255)
                                                              ccGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCa                           >  2:5048931/1‑93 (MQ=255)
                                                               cGCATCAAATACCGGCTGTTTTTCCTGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAg                          <  1:6086488/93‑1 (MQ=255)
                                                                gCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGc                         >  2:2970557/1‑93 (MQ=255)
                                                                gCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGc                         <  1:5382782/93‑1 (MQ=255)
                                                                 cATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCa                        >  1:6032206/1‑93 (MQ=255)
                                                                  aTCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCAt                       >  2:3339927/1‑93 (MQ=255)
                                                                   tCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATc                      >  1:83798/1‑93 (MQ=255)
                                                                   tCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATc                      <  1:6298281/93‑1 (MQ=255)
                                                                   tCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCAAGCATc                      >  2:2694256/1‑93 (MQ=255)
                                                                       aTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCt                  >  1:95991/1‑93 (MQ=255)
                                                                       aTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCt                  <  2:2740396/93‑1 (MQ=255)
                                                                        tACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTc                 <  2:1552515/93‑1 (MQ=255)
                                                                        tACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTc                 >  2:4213255/1‑93 (MQ=255)
                                                                        tACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTc                 <  2:6099342/93‑1 (MQ=255)
                                                                        tACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTc                 >  1:5674443/1‑93 (MQ=255)
                                                                        tACCGGCTGTGTTGCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTc                 <  1:729309/93‑1 (MQ=255)
                                                                           cGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGgc              <  2:3865552/93‑1 (MQ=255)
                                                                             gCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGgcgc            >  2:2914123/1‑93 (MQ=255)
                                                                             gCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGgcgc            >  2:3698928/1‑93 (MQ=255)
                                                                               tGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTg          >  1:5107288/1‑93 (MQ=255)
                                                                                gTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgc         >  1:3456605/1‑93 (MQ=255)
                                                                                gTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgc         >  2:4958849/1‑93 (MQ=255)
                                                                                  ttttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgcgc       >  2:2474940/1‑93 (MQ=255)
                                                                                    ttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAc     <  1:2263280/93‑1 (MQ=255)
                                                                                      ccAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgc   <  1:1849149/93‑1 (MQ=255)
                                                                                      ccAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgc   >  2:4889264/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC  >  NC_000913/4604742‑4604921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTAAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCGCTG  >  NC_000913/4604739‑4604925
                                                                                             |                                                                                             
GCTAAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGG                                                                                               <  SRR4302533.3598618/94‑1 (MQ=60)
  TAAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCG                                                                                             <  SRR4302533.1722396/94‑1 (MQ=60)
     ACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACC                                                                                          <  SRR4302533.1590107/94‑1 (MQ=60)
      CACGCCGGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCA                                                                                         <  SRR4302533.3386134/94‑1 (MQ=60)
      CACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCA                                                                                         <  SRR4302533.1059423/94‑1 (MQ=60)
       ACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAA                                                                                        >  SRR4302533.2232106/1‑94 (MQ=60)
          CCAGGCAGCGTTTTGACCGTCAGGCCTTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGTCCACCAAAAT                                                                                     >  SRR4302533.4284539/1‑94 (MQ=60)
          CCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAAT                                                                                     >  SRR4302533.1940991/1‑94 (MQ=60)
            AGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAG                                                                                   <  SRR4302533.4722047/94‑1 (MQ=60)
            AGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAG                                                                                   <  SRR4302533.2478035/94‑1 (MQ=60)
            AGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAG                                                                                   >  SRR4302533.4166021/1‑94 (MQ=60)
             GGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGA                                                                                  <  SRR4302533.1538090/94‑1 (MQ=60)
             GGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGA                                                                                  <  SRR4302533.1931870/94‑1 (MQ=60)
               CAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGG                                                                                >  SRR4302533.5240893/1‑94 (MQ=60)
               CAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGG                                                                                >  SRR4302533.2182734/1‑94 (MQ=60)
               CAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGG                                                                                <  SRR4302533.4491369/94‑1 (MQ=60)
               CAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGG                                                                                <  SRR4302533.6107937/94‑1 (MQ=60)
                TGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGC                                                                               <  SRR4302533.14286/94‑1 (MQ=60)
                AGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCGTCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGC                                                                               >  SRR4302533.2120190/1‑94 (MQ=60)
                AGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCACATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGC                                                                               >  SRR4302533.287289/1‑94 (MQ=60)
                 GCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCC                                                                              <  SRR4302533.2481185/94‑1 (MQ=60)
                 GCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCC                                                                              <  SRR4302533.2409536/94‑1 (MQ=60)
                  CGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCA                                                                             >  SRR4302533.2631959/1‑94 (MQ=60)
                   GTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCAC                                                                            >  SRR4302533.1098027/1‑94 (MQ=60)
                   GTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCAC                                                                            >  SRR4302533.2230232/1‑94 (MQ=60)
                     TTTGACCGTCAGGCCATCGATGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAG                                                                          <  SRR4302533.302714/94‑1 (MQ=60)
                     TTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAG                                                                          <  SRR4302533.3492458/94‑1 (MQ=60)
                     TTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAG                                                                          <  SRR4302533.5872691/94‑1 (MQ=60)
                      TTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGC                                                                         <  SRR4302533.4149625/94‑1 (MQ=60)
                       TGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACTGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCG                                                                        <  SRR4302533.2405601/94‑1 (MQ=60)
                       TGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCG                                                                        >  SRR4302533.3907558/1‑94 (MQ=60)
                         ACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGAC                                                                      <  SRR4302533.1202458/94‑1 (MQ=60)
                          CCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACG                                                                     <  SRR4302533.5383423/94‑1 (MQ=60)
                            GTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAG                                                                   >  SRR4302533.3365265/1‑94 (MQ=60)
                            GTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAG                                                                   >  SRR4302533.2476256/1‑94 (MQ=60)
                             TCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGC                                                                  <  SRR4302533.5102493/94‑1 (MQ=60)
                             TCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGC                                                                  <  SRR4302533.6110202/94‑1 (MQ=60)
                              CAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCG                                                                 >  SRR4302533.1933350/1‑94 (MQ=60)
                              CAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCG                                                                 >  SRR4302533.754328/1‑94 (MQ=60)
                               AGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGC                                                                >  SRR4302533.6050548/1‑94 (MQ=60)
                                GGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCT                                                               <  SRR4302533.1033344/94‑1 (MQ=60)
                                 GCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTG                                                              <  SRR4302533.5056509/94‑1 (MQ=60)
                                   CATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTC                                                            <  SRR4302533.1873260/94‑1 (MQ=60)
                                   CATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTC                                                            <  SRR4302533.5884170/94‑1 (MQ=60)
                                   CATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTC                                                            >  SRR4302533.488734/1‑94 (MQ=60)
                                   CATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTC                                                            <  SRR4302533.898990/94‑1 (MQ=60)
                                    ATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCG                                                           >  SRR4302533.3105443/1‑94 (MQ=60)
                                     TCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGA                                                          >  SRR4302533.3142482/1‑94 (MQ=60)
                                       GACGCTGTATTCGCCCCAGAATATCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGAAT                                                        <  SRR4302533.5466121/94‑1 (MQ=60)
                                        ACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTT                                                       <  SRR4302533.4253943/94‑1 (MQ=60)
                                        ACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTT                                                       >  SRR4302533.1268386/1‑94 (MQ=60)
                                        ACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTT                                                       <  SRR4302533.2175269/94‑1 (MQ=60)
                                         CGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTT                                                      <  SRR4302533.2546539/94‑1 (MQ=60)
                                         CGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTT                                                      >  SRR4302533.5977394/1‑94 (MQ=60)
                                          GCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTA                                                     >  SRR4302533.2378709/1‑94 (MQ=60)
                                          GCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTA                                                     >  SRR4302533.2178213/1‑94 (MQ=60)
                                           CTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAT                                                    >  SRR4302533.566967/1‑94 (MQ=60)
                                           CTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAT                                                    >  SRR4302533.2102406/1‑94 (MQ=60)
                                           CTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAT                                                    <  SRR4302533.4434737/94‑1 (MQ=60)
                                           CTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAT                                                    >  SRR4302533.5131765/1‑94 (MQ=60)
                                            TGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATT                                                   >  SRR4302533.3317853/1‑94 (MQ=60)
                                             GTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTC                                                  >  SRR4302533.942415/1‑94 (MQ=60)
                                             GTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTC                                                  >  SRR4302533.3837637/1‑94 (MQ=60)
                                             GTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTA                                                  >  SRR4302533.5195440/1‑94 (MQ=60)
                                              TATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCA                                                 <  SRR4302533.4251094/94‑1 (MQ=60)
                                               ATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAA                                                >  SRR4302533.4820889/1‑94 (MQ=60)
                                               ATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAA                                                <  SRR4302533.720597/94‑1 (MQ=60)
                                                TTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAAC                                               <  SRR4302533.107064/94‑1 (MQ=60)
                                                 TCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACG                                              <  SRR4302533.299511/94‑1 (MQ=60)
                                                 TCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACG                                              <  SRR4302533.4091381/94‑1 (MQ=60)
                                                   GCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGC                                            >  SRR4302533.1985803/1‑94 (MQ=60)
                                                   GCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGC                                            <  SRR4302533.4175995/94‑1 (MQ=60)
                                                    CCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCG                                           >  SRR4302533.854900/1‑94 (MQ=60)
                                                     CCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGC                                          >  SRR4302533.1088654/1‑94 (MQ=60)
                                                       CAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCAT                                        >  SRR4302533.4683195/1‑94 (MQ=60)
                                                         GAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAA                                      <  SRR4302533.4676895/94‑1 (MQ=60)
                                                         GAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAA                                      >  SRR4302533.2453531/1‑94 (MQ=60)
                                                         GAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAA                                      <  SRR4302533.3554098/94‑1 (MQ=60)
                                                          AATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAT                                     >  SRR4302533.5604409/1‑94 (MQ=60)
                                                          AATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAT                                     <  SRR4302533.1403048/94‑1 (MQ=60)
                                                           ATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATC                                    <  SRR4302533.6049310/94‑1 (MQ=60)
                                                           ATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATC                                    >  SRR4302533.3020236/1‑94 (MQ=60)
                                                           ATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATC                                    <  SRR4302533.1027431/94‑1 (MQ=60)
                                                           ATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATC                                    <  SRR4302533.4901369/94‑1 (MQ=60)
                                                             TTCTCCGCATCAAATACCGGCTGTTTTTCCATGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTG                                  <  SRR4302533.2430656/94‑1 (MQ=60)
                                                               CTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCC                                >  SRR4302533.848833/1‑94 (MQ=60)
                                                                TCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCA                               >  SRR4302533.5840924/1‑94 (MQ=60)
                                                                TCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCA                               >  SRR4302533.2752510/1‑94 (MQ=60)
                                                                TCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCA                               >  SRR4302533.1220895/1‑94 (MQ=60)
                                                                 CCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAG                              >  SRR4302533.5452594/1‑94 (MQ=60)
                                                                  CGCATCAAATACCGGCTGTTTTTCCTGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGC                             <  SRR4302533.6123284/94‑1 (MQ=60)
                                                                   GCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCA                            >  SRR4302533.6068764/1‑94 (MQ=60)
                                                                   GCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCA                            <  SRR4302533.5415121/94‑1 (MQ=60)
                                                                     ATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATC                          >  SRR4302533.6147816/1‑94 (MQ=60)
                                                                     ATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATC                          >  SRR4302533.2735981/1‑94 (MQ=60)
                                                                     ATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATC                          >  SRR4302533.84331/1‑94 (MQ=60)
                                                                      TCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCT                         <  SRR4302533.6335875/94‑1 (MQ=60)
                                                                      TCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCT                         <  SRR4302533.1629329/94‑1 (MQ=60)
                                                                       CAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTG                        >  SRR4302533.5825924/1‑94 (MQ=60)
                                                                       CAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTG                        >  SRR4302533.2082350/1‑94 (MQ=60)
                                                                       CAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTG                        >  SRR4302533.1336676/1‑94 (MQ=60)
                                                                        AAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGC                       <  SRR4302533.4047021/94‑1 (MQ=60)
                                                                         AATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCT                      >  SRR4302533.96594/1‑94 (MQ=60)
                                                                         AATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCT                      <  SRR4302533.5796309/94‑1 (MQ=60)
                                                                          ATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTC                     >  SRR4302533.5708712/1‑94 (MQ=60)
                                                                           TACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCG                    <  SRR4302533.2193268/94‑1 (MQ=60)
                                                                           TACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCG                    <  SRR4302533.3667231/94‑1 (MQ=60)
                                                                           TACCGGCTGTGTTGCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCG                    <  SRR4302533.733612/94‑1 (MQ=60)
                                                                            ACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGG                   <  SRR4302533.4964512/94‑1 (MQ=60)
                                                                            ACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGG                   <  SRR4302533.5147591/94‑1 (MQ=60)
                                                                            ACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGG                   >  SRR4302533.1187038/1‑94 (MQ=60)
                                                                             CCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGC                  >  SRR4302533.661389/1‑94 (MQ=60)
                                                                               GGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGC                >  SRR4302533.1595536/1‑94 (MQ=60)
                                                                                 CTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTG              >  SRR4302533.5138145/1‑94 (MQ=60)
                                                                                 CTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTG              >  SRR4302533.5911297/1‑94 (MQ=60)
                                                                                  TGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGC             >  SRR4302533.3477944/1‑94 (MQ=60)
                                                                                  TGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGC             >  SRR4302533.4120021/1‑94 (MQ=60)
                                                                                       TTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACG        <  SRR4302533.2277285/94‑1 (MQ=60)
                                                                                         CCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC      >  SRR4302533.2134315/1‑94 (MQ=60)
                                                                                         CCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC      <  SRR4302533.1860429/94‑1 (MQ=60)
                                                                                          CAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCG     <  SRR4302533.2884503/94‑1 (MQ=60)
                                                                                          CAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCG     <  SRR4302533.2207266/94‑1 (MQ=60)
                                                                                           AGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCGC    >  SRR4302533.400031/1‑94 (MQ=60)
                                                                                            GGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCGCT   >  SRR4302533.3891377/1‑94 (MQ=60)
                                                                                            GGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCGCT   >  SRR4302533.3891378/1‑94 (MQ=60)
                                                                                            GGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCGCT   >  SRR4302533.2060623/1‑94 (MQ=60)
                                                                                             GCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCGCTG  <  SRR4302533.1940074/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCTAAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCCGCTG  >  NC_000913/4604739‑4604925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑