Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,237,247 +C intergenic (+60/‑337) ygjT → / → ygjU putative transport protein/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,237,2451.C99.4% 1039.0 / ‑1.9 307intergenic (+58/‑339)ygjT/ygjUputative transport protein/putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (242/63);  total (242/65)
Fisher's exact test for biased strand distribution p-value = 4.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

AATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CT‑CCTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGC  >  NC_000913/3237155‑3237333
                                                                                             |                                                                                        
aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑ctccctct‑cc                                                                                        >  1:7188991/1‑94 (MQ=255)
aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑ctccctct‑cc                                                                                        >  2:8411488/1‑94 (MQ=255)
 aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑ctccctct‑ccc                                                                                       <  1:10306373/94‑2 (MQ=255)
 aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑ctccctct‑ccc                                                                                       <  1:1535472/94‑2 (MQ=255)
   tATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑ctccctct‑ccctt                                                                                     >  1:9200886/1‑91 (MQ=255)
       ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAg                                                                                  <  1:8050025/94‑1 (MQ=255)
       ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAg                                                                                  <  2:6550676/94‑1 (MQ=255)
       ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAg                                                                                  >  2:2045167/1‑94 (MQ=255)
       ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAg                                                                                  >  1:3656841/1‑94 (MQ=255)
        gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAgg                                                                                 <  1:8226673/94‑1 (MQ=255)
         cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAggg                                                                                >  1:10174648/1‑94 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTGCCTCTCCCTTCCAGGga                                                                               <  1:9566498/94‑1 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGga                                                                               >  2:5969786/1‑94 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGga                                                                               >  1:2570050/1‑94 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGga                                                                               <  1:4068461/94‑1 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGga                                                                               >  1:936247/1‑94 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGga                                                                               >  1:1970994/1‑94 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGCga                                                                               >  1:3474851/1‑94 (MQ=255)
          aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACACGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGga                                                                               >  1:3111902/1‑94 (MQ=255)
           tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGgag                                                                              >  2:3122819/1‑94 (MQ=255)
           tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGgag                                                                              >  1:1473390/1‑94 (MQ=255)
           tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGgag                                                                              >  1:5182858/1‑94 (MQ=255)
           tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGgag                                                                              >  2:459382/1‑94 (MQ=255)
           tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGgag                                                                              <  2:5628743/94‑1 (MQ=255)
             aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGagag                                                                            >  1:9125422/1‑94 (MQ=255)
              tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAgg                                                                           <  2:3349462/94‑1 (MQ=255)
               aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAggg                                                                          >  2:6249226/1‑94 (MQ=255)
               aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAggg                                                                          >  1:4923614/1‑94 (MQ=255)
                agcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGt                                                                         >  2:6351391/1‑94 (MQ=255)
                agcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGt                                                                         >  2:8480899/1‑94 (MQ=255)
                  cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCGCCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                       >  1:6900376/1‑94 (MQ=255)
                  cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                       <  2:1515379/94‑1 (MQ=255)
                  cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                       <  2:1157906/94‑1 (MQ=255)
                  cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCGGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                       <  2:5390083/94‑1 (MQ=255)
                   agcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                      >  1:6063391/1‑94 (MQ=255)
                   agcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                      >  2:8266838/1‑94 (MQ=255)
                    gcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCggg                                                                     <  1:6592299/94‑1 (MQ=255)
                     cGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCgggg                                                                    >  2:5890107/1‑94 (MQ=255)
                     cGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCgggg                                                                    >  2:8153826/1‑94 (MQ=255)
                     cGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCgggg                                                                    >  1:8667310/1‑94 (MQ=255)
                      ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCggggg                                                                   >  2:1362048/1‑93 (MQ=255)
                      ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                   <  2:8788457/94‑1 (MQ=255)
                       gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTg                                                                  <  1:6842401/94‑1 (MQ=255)
                       gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTg                                                                  >  2:1058403/1‑94 (MQ=255)
                       gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGAGTg                                                                  >  2:642766/1‑94 (MQ=255)
                          tGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                               <  1:8571323/94‑1 (MQ=255)
                           ggATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑ATCCCTCTCCCTTAAAGGGAGAGGGTCGGGGTGAggg                                                              <  2:1937303/94‑1 (MQ=255)
                            gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGt                                                             >  1:6715987/1‑94 (MQ=38)
                            gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGt                                                             >  1:4474481/1‑94 (MQ=38)
                            gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGGCGGGGTGAggga                                                             >  2:7666061/1‑93 (MQ=38)
                             aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGCGAGGGTCGGGGTGAGGGTa                                                            >  2:1740689/1‑94 (MQ=38)
                               aaTTTTTAATCTGCCTAAGACGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAt                                                          >  2:5832448/1‑94 (MQ=38)
                                aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                         >  2:8567640/1‑94 (MQ=38)
                                aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                         >  1:8866106/1‑94 (MQ=38)
                                  ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                       >  2:527402/1‑94 (MQ=38)
                                   tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                      >  2:9656975/1‑94 (MQ=38)
                                   tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                      >  2:7843850/1‑94 (MQ=38)
                                   tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                      >  2:3404006/1‑94 (MQ=38)
                                    ttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTc                                                     >  1:3173727/1‑94 (MQ=38)
                                     tAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                    >  2:5530931/1‑94 (MQ=38)
                                     tAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGAACCGTTTT‑CTCCCTCTCCCTTCCAGGGAACGGGTCCGGCGGAGGCTAATTTGTCg                                                    >  2:4414503/1‑94 (MQ=255)
                                      aaTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGc                                                   >  1:99929/1‑94 (MQ=38)
                                      aaTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTAGc                                                   >  2:3700685/1‑94 (MQ=37)
                                           gCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGa                                              >  2:7140093/1‑94 (MQ=38)
                                            ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                             >  2:6975200/1‑94 (MQ=38)
                                             cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                            >  1:9135872/1‑94 (MQ=38)
                                             cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                            >  1:9815723/1‑94 (MQ=38)
                                             cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                            >  2:2842762/1‑94 (MQ=38)
                                             cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                            >  2:10251962/1‑94 (MQ=38)
                                             cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGCAATTTTTCGCACCGATg                                            >  2:8920875/1‑94 (MQ=38)
                                              tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGc                                           >  2:6743239/1‑94 (MQ=38)
                                               aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGCGGGTCTGGGTGAGGGTAATTTTTCGCACAGATGCt                                          >  2:10455239/1‑94 (MQ=38)
                                               aaGCCGTGCACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCt                                          >  2:9210638/1‑94 (MQ=37)
                                                  ccGTGTACCCTGTCATTAACATGAGCACCGTTTTCCC‑CCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGc                                       >  1:10264484/1‑94 (MQ=37)
                                                   cGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                      >  1:7456948/1‑94 (MQ=37)
                                                   cGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                      >  1:5640950/1‑94 (MQ=37)
                                                   cGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGGAATTTTTCGCACCGATGCTGGcc                                      >  2:355980/1‑94 (MQ=37)
                                                   cGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGGCGGGGGGAGGGGACTATTTTGCACCGATGCTGGcc                                      >  1:4290211/1‑94 (MQ=255)
                                                     tgtACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                    >  1:5317055/1‑94 (MQ=37)
                                                     tgtACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                    >  1:7695640/1‑94 (MQ=37)
                                                      gtACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGt                                   >  2:1703129/1‑94 (MQ=38)
                                                       tACCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                  >  2:1865578/1‑94 (MQ=38)
                                                        aCCCTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                 >  1:10604384/1‑94 (MQ=37)
                                                         cccTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcc                                >  2:5125054/1‑94 (MQ=37)
                                                          ccTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                               >  1:10006651/1‑94 (MQ=37)
                                                          ccTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGCAATTTTTCGCACCGATGCTGGCCTGTTtcc                               >  1:6225031/1‑94 (MQ=37)
                                                           cTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtttcac                              >  2:2321363/1‑90 (MQ=25)
                                                           cTGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                              >  2:6695079/1‑94 (MQ=25)
                                                            tGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTtcccc                             >  2:413273/1‑93 (MQ=37)
                                                            tGTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTtcccc                             >  1:6429300/1‑93 (MQ=37)
                                                             gTCATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccccc                            >  2:2616524/1‑92 (MQ=37)
                                                               cATTAACATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAc                          >  1:1774754/1‑94 (MQ=25)
                                                                    aCATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCCCCCCCCaa                     >  1:4684648/1‑94 (MQ=21)
                                                                     cATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccccccccccccac                    >  1:554880/1‑90 (MQ=25)
                                                                          gCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct               >  2:7334354/1‑94 (MQ=18)
                                                                           cACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTCACCCtctc              >  2:4861578/1‑94 (MQ=18)
                                                                              cGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTcccc           >  1:10356787/1‑94 (MQ=18)
                                                                               gTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCCCCCCCCAACCCTCTCCCCa          >  1:3015678/1‑94 (MQ=18)
                                                                               gTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCCCACCCCAACCCTCTCCCCa          >  2:9895035/1‑94 (MQ=18)
                                                                                        cctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAACCTCTTCCCCAACGGGGc  >  2:5067358/1‑94 (MQ=18)
                                                                                             |                                                                                        
AATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTT‑CT‑CCTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGC  >  NC_000913/3237155‑3237333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 236 total aligned reads displayed.
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  NC_000913/3253649‑3253835
                                                                                             |                                                                                             
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGT                                                                                               >  SRR4302545.3563701/1‑94 (MQ=60)
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGT                                                                                               <  SRR4302545.588074/94‑1 (MQ=60)
 TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              <  SRR4302545.5180922/94‑1 (MQ=60)
 TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              <  SRR4302545.6791150/94‑1 (MQ=60)
 TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              <  SRR4302545.10810865/94‑1 (MQ=60)
 TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTT                                                                                              >  SRR4302545.798606/1‑94 (MQ=60)
  AACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTC                                                                                            >  SRR4302545.1589030/1‑94 (MQ=60)
   ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCT                                                                                           >  SRR4302545.10129472/1‑94 (MQ=60)
   ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCT                                                                                           <  SRR4302545.10247654/94‑1 (MQ=60)
    CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTG                                                                                          <  SRR4302545.3583423/94‑1 (MQ=60)
    CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTG                                                                                          >  SRR4302545.9269076/1‑94 (MQ=60)
     GCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGG                                                                                         >  SRR4302545.3656346/1‑94 (MQ=60)
     GCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGG                                                                                         >  SRR4302545.4543717/1‑94 (MQ=60)
     GCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGG                                                                                         >  SRR4302545.5522875/1‑94 (MQ=60)
     GCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGG                                                                                         <  SRR4302545.7750740/94‑1 (MQ=60)
        CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAA                                                                                      <  SRR4302545.10831558/94‑1 (MQ=60)
        CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAA                                                                                      <  SRR4302545.801127/94‑1 (MQ=60)
         AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     >  SRR4302545.3329908/1‑94 (MQ=60)
         AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     <  SRR4302545.9945653/94‑1 (MQ=60)
         AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     <  SRR4302545.9254241/94‑1 (MQ=60)
         AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     >  SRR4302545.372857/1‑94 (MQ=60)
         AATTTAGCTGCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     <  SRR4302545.9254242/94‑1 (MQ=60)
         AATGTAGCTTCGCGGAATCGAGAATAAAGCGGTTCGACGCAAACGGGACTTCATTGACGAACTTCAGGATATCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     <  SRR4302545.6660814/94‑1 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    >  SRR4302545.10436885/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    >  SRR4302545.2318469/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    <  SRR4302545.956411/94‑1 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    >  SRR4302545.10359908/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGGC‑GTTCTGGAAAGC                                                                                    >  SRR4302545.3320180/1‑94 (MQ=60)
           TTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCA                                                                                   >  SRR4302545.59951/1‑94 (MQ=60)
            TTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAC                                                                                  >  SRR4302545.6368940/1‑94 (MQ=60)
            TTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAC                                                                                  <  SRR4302545.7344894/94‑1 (MQ=60)
             acactctttccctacacgacgctcttccgatctctgatctGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                 <  SRR4302545.5838320/54‑1 (MQ=60)
              AGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCG                                                                                >  SRR4302545.10302812/1‑94 (MQ=60)
              AGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCAATGACCCACTTCAGGATCTCAGTCAGCGTC‑GTTCCGGAAAGCACCG                                                                                >  SRR4302545.9426149/1‑94 (MQ=60)
              AGCCTCGTGGAATCGAGAATAAAGCGGATCGCCGCAATCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCG                                                                                <  SRR4302545.8995173/94‑1 (MQ=60)
                CTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTC                                                                              >  SRR4302545.5908655/1‑94 (MQ=60)
                 TTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCA                                                                             <  SRR4302545.5396231/94‑1 (MQ=60)
                 TTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCA                                                                             <  SRR4302545.9324058/94‑1 (MQ=60)
                 TTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCA                                                                             <  SRR4302545.5236822/94‑1 (MQ=60)
                 TTCGCGGAATCGAAAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCA                                                                             <  SRR4302545.9324057/94‑1 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                            <  SRR4302545.4568378/94‑1 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                            <  SRR4302545.794685/94‑1 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                            <  SRR4302545.9752908/94‑1 (MQ=60)
                   CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGG                                                                           >  SRR4302545.1870302/1‑94 (MQ=60)
                   CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGC                                                                           <  SRR4302545.8491462/94‑1 (MQ=60)
                   CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGC                                                                           >  SRR4302545.7874534/1‑94 (MQ=60)
                    GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTTGGAAGCACCGTCAGCG                                                                          >  SRR4302545.9898634/1‑94 (MQ=60)
                    GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCG                                                                          >  SRR4302545.778343/1‑94 (MQ=60)
                     cggatcaGAGAATAAAGCGAATCGCCGCACACGGGCCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCGGGAAAGCACCGTCAGCGG                                                                         <  SRR4302545.679523/87‑1 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGG‑TCTGGAAAGCACCGCCAGCGG                                                                         >  SRR4302545.3471871/1‑94 (MQ=60)
                      GGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGA                                                                        >  SRR4302545.2498830/1‑94 (MQ=60)
                      GGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGA                                                                        >  SRR4302545.2703462/1‑94 (MQ=60)
                      GGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGA                                                                        <  SRR4302545.791498/94‑1 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       <  SRR4302545.10619916/94‑1 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       >  SRR4302545.8150239/1‑94 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       <  SRR4302545.8421041/94‑1 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       >  SRR4302545.2004260/1‑94 (MQ=60)
                       GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       >  SRR4302545.10508007/1‑94 (MQ=60)
                        AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGC                                                                      >  SRR4302545.2608518/1‑94 (MQ=60)
                        AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGA                                                                      >  SRR4302545.4825681/1‑94 (MQ=60)
                        AATCGAGAATAAAGCGGATCGCCCCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGA                                                                      >  SRR4302545.10374134/1‑94 (MQ=60)
                         ATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAC                                                                     <  SRR4302545.33129/94‑1 (MQ=60)
                         ATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAC                                                                     <  SRR4302545.965821/94‑1 (MQ=60)
                          TCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACT                                                                    >  SRR4302545.9716768/1‑94 (MQ=60)
                          TCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACT                                                                    <  SRR4302545.4956391/94‑1 (MQ=60)
                          TCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACT                                                                    <  SRR4302545.4956390/94‑1 (MQ=60)
                           CGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGGGTC‑GTTCTGGAAAGCACCGCCAGCGGAGACTA                                                                   >  SRR4302545.3214551/1‑94 (MQ=60)
                           CGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTC                                                                   >  SRR4302545.5138655/1‑94 (MQ=60)
                           CGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAAGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCTGAGACTC                                                                   >  SRR4302545.1192925/1‑94 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                  <  SRR4302545.4289318/94‑1 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 >  SRR4302545.7874759/1‑94 (MQ=60)
                              GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                <  SRR4302545.9344969/94‑1 (MQ=60)
                              GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                >  SRR4302545.10487538/1‑94 (MQ=60)
                              GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                >  SRR4302545.8498692/1‑94 (MQ=60)
                              GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                >  SRR4302545.5992122/1‑94 (MQ=60)
                               AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGC                                                               <  SRR4302545.421069/94‑1 (MQ=60)
                               AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGC                                                               >  SRR4302545.5867443/1‑94 (MQ=60)
                                ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                              >  SRR4302545.9957079/1‑94 (MQ=60)
                                ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                              <  SRR4302545.5051455/94‑1 (MQ=60)
                                ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                              >  SRR4302545.558385/1‑94 (MQ=60)
                                ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                              <  SRR4302545.8665677/94‑1 (MQ=60)
                                 TAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTC                                                             <  SRR4302545.7658480/94‑1 (MQ=60)
                                 TAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTC                                                             <  SRR4302545.1164677/94‑1 (MQ=60)
                                  AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            >  SRR4302545.2526667/1‑94 (MQ=60)
                                  AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            <  SRR4302545.3367774/94‑1 (MQ=60)
                                  AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            <  SRR4302545.74299/94‑1 (MQ=60)
                                    AGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCC                                                          >  SRR4302545.3568729/1‑94 (MQ=60)
                                     GCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCC                                                         <  SRR4302545.1129120/94‑1 (MQ=60)
                                      CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATTTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCCGAGACTCTTGCTCGCCCT                                                        >  SRR4302545.3619337/1‑94 (MQ=60)
                                      CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTTCTCCCCCT                                                        >  SRR4302545.10785598/1‑94 (MQ=60)
                                      CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCT                                                        >  SRR4302545.3603715/1‑94 (MQ=60)
                                       GGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTC                                                       <  SRR4302545.6178837/94‑1 (MQ=60)
                                       GGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTC                                                       >  SRR4302545.9850983/1‑94 (MQ=60)
                                       GGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTC                                                       >  SRR4302545.5470716/1‑94 (MQ=60)
                                        GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                      <  SRR4302545.4959011/94‑1 (MQ=60)
                                        GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGT‑AGTTCTGGAAAGCACCGTCAGAGGAGACTCTTGCTCGACCTCT                                                      >  SRR4302545.5298349/1‑94 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCcgcgtaaaccctagcgcgccctctg                                                     >  SRR4302545.10113698/1‑69 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     >  SRR4302545.3887812/1‑94 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     <  SRR4302545.9175162/94‑1 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     <  SRR4302545.4523997/94‑1 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     >  SRR4302545.5128386/1‑94 (MQ=60)
                                          TCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGC                                                    <  SRR4302545.1249118/94‑1 (MQ=60)
                                           CGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCC                                                   >  SRR4302545.33000/1‑94 (MQ=60)
                                            GCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                  >  SRR4302545.4609776/1‑94 (MQ=60)
                                              CGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGGGTC‑GTTCTGGAAAGCACCGTCAGCGGAGCCTCTGGCTCGCCCTCTGCCACC                                                >  SRR4302545.1229589/1‑94 (MQ=60)
                                              CGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                                >  SRR4302545.1229590/1‑94 (MQ=60)
                                               GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACAC                                               >  SRR4302545.4870529/1‑94 (MQ=60)
                                                CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              >  SRR4302545.7720335/1‑94 (MQ=60)
                                                CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              >  SRR4302545.7844124/1‑94 (MQ=60)
                                                CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              >  SRR4302545.1520154/1‑94 (MQ=60)
                                                CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              <  SRR4302545.7089037/94‑1 (MQ=60)
                                                CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              >  SRR4302545.5314996/1‑94 (MQ=60)
                                                CAAACGGGACTGCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACA                                              <  SRR4302545.7089039/94‑1 (MQ=60)
                                                 AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACccag                                             >  SRR4302545.3286760/1‑90 (MQ=60)
                                                 AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACAC                                             >  SRR4302545.9418262/1‑94 (MQ=60)
                                                  AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACG                                            <  SRR4302545.5065157/94‑1 (MQ=60)
                                                  AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACG                                            >  SRR4302545.8267692/1‑94 (MQ=60)
                                                   ACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGC                                           <  SRR4302545.3016613/94‑1 (MQ=60)
                                                   ACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGC                                           <  SRR4302545.3016612/94‑1 (MQ=60)
                                                    CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCC                                          >  SRR4302545.8454753/1‑94 (MQ=60)
                                                    CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCC                                          >  SRR4302545.1911063/1‑94 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         >  SRR4302545.3141132/1‑94 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         >  SRR4302545.3093168/1‑94 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         <  SRR4302545.5250538/94‑1 (MQ=60)
                                                     GGGACTTCATTGACGAACTGCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         <  SRR4302545.5250537/94‑1 (MQ=60)
                                                       GACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGC                                       >  SRR4302545.9850124/1‑94 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302545.802117/1‑94 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302545.10574975/1‑94 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302545.2208945/1‑94 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302545.5648460/1‑94 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      <  SRR4302545.9145388/94‑1 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      <  SRR4302545.8925352/94‑1 (MQ=60)
                                                         CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                     >  SRR4302545.5847794/1‑94 (MQ=60)
                                                          TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                    >  SRR4302545.4039261/1‑94 (MQ=60)
                                                          TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                    >  SRR4302545.7327750/1‑94 (MQ=60)
                                                           TCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                   >  SRR4302545.177256/1‑94 (MQ=60)
                                                            CATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGT                                  >  SRR4302545.6549653/1‑94 (MQ=60)
                                                            CATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGG                                  >  SRR4302545.6549654/1‑94 (MQ=60)
                                                             ATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTA                                 <  SRR4302545.6097785/94‑1 (MQ=60)
                                                             ATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTA                                 >  SRR4302545.1275903/1‑94 (MQ=60)
                                                             ATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGCA                                 >  SRR4302545.77451/1‑94 (MQ=60)
                                                              TTGACGAACTTCAGGGTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAA                                <  SRR4302545.10541189/94‑1 (MQ=60)
                                                              TTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAA                                <  SRR4302545.10157663/94‑1 (MQ=60)
                                                               TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                               >  SRR4302545.881749/1‑94 (MQ=60)
                                                                GACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                              <  SRR4302545.3198306/94‑1 (MQ=60)
                                                                GACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                              >  SRR4302545.371046/1‑94 (MQ=60)
                                                                GACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                              >  SRR4302545.9464134/1‑94 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                             >  SRR4302545.10571070/1‑94 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                             >  SRR4302545.5510341/1‑94 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                             >  SRR4302545.10571071/1‑94 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                             >  SRR4302545.9134289/1‑94 (MQ=60)
                                                                  CGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                            >  SRR4302545.2404864/1‑94 (MQ=60)
                                                                   GAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACC                           >  SRR4302545.3862363/1‑94 (MQ=60)
                                                                    AACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCC                          >  SRR4302545.1721278/1‑94 (MQ=60)
                                                                    AACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCA                          <  SRR4302545.1746944/94‑1 (MQ=60)
                                                                     ACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCAC                         <  SRR4302545.6305701/94‑1 (MQ=60)
                                                                     ACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCAC                         <  SRR4302545.6305700/94‑1 (MQ=60)
                                                                      CTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACA                        >  SRR4302545.5963014/1‑94 (MQ=60)
                                                                       TTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                       <  SRR4302545.1100478/94‑1 (MQ=60)
                                                                          AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                    >  SRR4302545.6408837/1‑94 (MQ=60)
                                                                          AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                    >  SRR4302545.7310740/1‑94 (MQ=60)
                                                                          AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                    <  SRR4302545.10836883/94‑1 (MQ=60)
                                                                          AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                    >  SRR4302545.7310739/1‑94 (MQ=60)
                                                                           GGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                   <  SRR4302545.8728752/94‑1 (MQ=60)
                                                                           GGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                   <  SRR4302545.3611545/94‑1 (MQ=60)
                                                                            GATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                  <  SRR4302545.4316712/94‑1 (MQ=60)
                                                                             GTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                 <  SRR4302545.3876731/94‑1 (MQ=60)
                                                                             ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAttctcgt                 >  SRR4302545.4496568/1‑87 (MQ=60)
                                                                             ATCTCAGCCAGCGGC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCGACACACGCCTGCTGGGTAAACACCACAACATCGT                 >  SRR4302545.4496567/1‑94 (MQ=60)
                                                                              TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG                <  SRR4302545.2627710/94‑1 (MQ=60)
                                                                               CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC               <  SRR4302545.1305776/94‑1 (MQ=60)
                                                                               CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC               <  SRR4302545.7022519/94‑1 (MQ=60)
                                                                               CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC               <  SRR4302545.9173813/94‑1 (MQ=60)
                                                                                 CAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT             >  SRR4302545.399445/1‑94 (MQ=60)
                                                                                  AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC            <  SRR4302545.6711167/94‑1 (MQ=60)
                                                                                   GTCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCTCTCTGCCACACATGCCTGCTGGGTAAACACCTCACCATCGTGCGTCT           >  SRR4302545.9644851/1‑94 (MQ=60)
                                                                                   GCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCT           >  SRR4302545.5948623/1‑94 (MQ=60)
                                                                                   GCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCT           <  SRR4302545.7993388/94‑1 (MQ=60)
                                                                                    CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC          >  SRR4302545.9411784/1‑94 (MQ=60)
                                                                                    CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC          >  SRR4302545.3838202/1‑94 (MQ=60)
                                                                                    CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCACCGTGCGTCTC          >  SRR4302545.9411785/1‑94 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         <  SRR4302545.8121507/94‑1 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         >  SRR4302545.6773413/1‑94 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         <  SRR4302545.5479420/94‑1 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         >  SRR4302545.6710538/1‑94 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         <  SRR4302545.1645994/94‑1 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         >  SRR4302545.1739821/1‑94 (MQ=60)
                                                                                      AGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA        >  SRR4302545.9287543/1‑94 (MQ=60)
                                                                                       GCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT       <  SRR4302545.4232100/94‑1 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA      >  SRR4302545.6311216/1‑94 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA      >  SRR4302545.2417447/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT     >  SRR4302545.8992616/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT     <  SRR4302545.10277933/94‑1 (MQ=60)
                                                                                           tcGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATG    <  SRR4302545.1402882/92‑1 (MQ=60)
                                                                                           tcGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATG    <  SRR4302545.3798239/92‑1 (MQ=60)
                                                                                           gcGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATG    <  SRR4302545.9052774/92‑1 (MQ=60)
                                                                                            CGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGC   >  SRR4302545.10377290/1‑94 (MQ=60)
                                                                                            CGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGC   <  SRR4302545.8335018/94‑1 (MQ=60)
                                                                                             GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  SRR4302545.4279380/1‑94 (MQ=60)
                                                                                             GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  SRR4302545.815070/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  NC_000913/3253649‑3253835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑