Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,814,781 T→A L160Q (CTG→CAG)  yicC → putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,814,7810TA99.6% 2860.0 / ‑0.8 732L160Q (CTG→CAG) yicCputative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (377/351);  total (379/353)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 751 total aligned reads displayed.

ATTCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA  >  NC_000913/3814690‑3814871
                                                                                           |                                                                                          
 ttCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGa                                                                                         >  1:1025124/1‑94 (MQ=255)
  tctcGCGGCGCTGGGTTGTTCGGTGGACGAATTTATTGGCGCGGGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGaa                                                                                        <  1:5215507/94‑1 (MQ=255)
  tctcGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGaa                                                                                        <  1:6315787/94‑1 (MQ=255)
   ctcGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAg                                                                                       <  2:4657804/94‑1 (MQ=255)
     cgcgGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGc                                                                                     <  2:5248379/94‑1 (MQ=255)
      gcggcgCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCg                                                                                    <  1:9815727/94‑1 (MQ=255)
       cggcgCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGt                                                                                   >  2:8032520/1‑94 (MQ=255)
        ggcgCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTc                                                                                  >  1:3448077/1‑94 (MQ=255)
        ggcgCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTc                                                                                  >  1:8925569/1‑94 (MQ=255)
         gcgcTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCa                                                                                 <  1:403606/94‑1 (MQ=255)
          cgcTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCAc                                                                                >  1:2752583/1‑94 (MQ=255)
           gcgggATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCAcc                                                                               <  1:6187919/91‑1 (MQ=255)
           gcTGGATGGTACGCTGGACGTCTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCAcc                                                                               >  2:3871946/1‑94 (MQ=255)
            cTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCAccg                                                                              <  2:1589778/94‑1 (MQ=255)
              ggATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCAccgcc                                                                            >  1:10379690/1‑94 (MQ=255)
              ggATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCAccgcc                                                                            <  1:6537402/94‑1 (MQ=255)
               gATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACAGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCAccgccg                                                                           <  1:9683864/94‑1 (MQ=255)
                  ggTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAg                                                                        <  1:1471393/94‑1 (MQ=255)
                   gTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAgg                                                                       >  2:1299211/1‑93 (MQ=255)
                    tACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTg                                                                      <  2:10623064/94‑1 (MQ=255)
                       gCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTc                                                                   >  1:7727252/1‑94 (MQ=255)
                        cTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCa                                                                  >  2:2224401/1‑94 (MQ=255)
                        cTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCa                                                                  >  2:2847347/1‑94 (MQ=255)
                         tGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCaa                                                                 >  1:7276729/1‑94 (MQ=255)
                         tGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCaa                                                                 >  2:4073801/1‑94 (MQ=255)
                         tGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCaa                                                                 >  2:9435003/1‑94 (MQ=255)
                           gacgacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAg                                                               <  2:8900798/94‑1 (MQ=255)
                            acgacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGt                                                              >  1:4954144/1‑94 (MQ=255)
                            acgacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGt                                                              <  1:1130027/94‑1 (MQ=255)
                             cgacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTc                                                             <  1:198387/94‑1 (MQ=255)
                              gacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTcc                                                            >  2:5403994/1‑94 (MQ=255)
                              gacTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTcc                                                            <  2:3345635/94‑1 (MQ=255)
                               acTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCg                                                           >  2:817796/1‑94 (MQ=255)
                                cTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGc                                                          <  1:9097721/94‑1 (MQ=255)
                                cTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGc                                                          >  1:1632467/1‑94 (MQ=255)
                                 tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCt                                                         <  1:2870862/94‑1 (MQ=255)
                                 tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCt                                                         <  2:6257473/94‑1 (MQ=255)
                                   tATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTcc                                                       >  2:5923355/1‑94 (MQ=255)
                                      tGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCat                                                    <  1:9515395/94‑1 (MQ=255)
                                       gTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCata                                                   >  1:9236110/1‑94 (MQ=255)
                                        tCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCatat                                                  <  1:3297596/94‑1 (MQ=255)
                                         cgcgcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATg                                                 <  2:41486/94‑1 (MQ=255)
                                           cgcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGcc                                               <  2:103024/94‑1 (MQ=255)
                                             cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCgg                                             >  1:5050622/1‑94 (MQ=255)
                                              gcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGa                                            <  2:8650083/94‑1 (MQ=255)
                                                gAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGaaa                                          >  2:3177153/1‑94 (MQ=255)
                                                gAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGaaa                                          >  1:8772705/1‑94 (MQ=255)
                                                gAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACAGCCGAAGTGGTAAAAGTCCGCTCCCATATGCCGGaaa                                          >  2:7807925/1‑94 (MQ=255)
                                                 aaaCCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAAt                                         >  1:6424999/1‑94 (MQ=255)
                                                  aaCCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATc                                        >  1:3069289/1‑94 (MQ=255)
                                                   aCCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATcc                                       <  1:8292112/94‑1 (MQ=255)
                                                    ccGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCt                                      >  1:10003983/1‑94 (MQ=255)
                                                    ccGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCt                                      >  2:1986281/1‑94 (MQ=255)
                                                     cGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTg                                     >  1:2419087/1‑94 (MQ=255)
                                                      gAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGc                                    >  2:7646801/1‑94 (MQ=255)
                                                       aaGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCa                                   >  2:5660722/1‑94 (MQ=255)
                                                       aaGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCa                                   >  2:834547/1‑94 (MQ=255)
                                                       aaGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCa                                   >  2:8495076/1‑94 (MQ=255)
                                                         ggTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAAt                                 >  1:4618311/1‑94 (MQ=255)
                                                         ggTCAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAAt                                 <  2:368020/94‑1 (MQ=255)
                                                            cAGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATgga                              >  2:246748/1‑93 (MQ=255)
                                                             aGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCa                             <  1:2558502/94‑1 (MQ=255)
                                                             aGGCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCa                             <  1:813946/94‑1 (MQ=255)
                                                              ggCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAg                            >  2:9600610/1‑94 (MQ=255)
                                                               gCACTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGc                           >  1:4187474/1‑94 (MQ=255)
                                                                 aCTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGt                         <  1:8518843/94‑1 (MQ=255)
                                                                 aCTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGt                         >  2:9258145/1‑94 (MQ=255)
                                                                  cTGAAAGCATTGATGGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGCAATCCTGCAATGGCAGCGTg                        >  1:1803377/1‑94 (MQ=255)
                                                                  cTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                        >  2:1414995/1‑94 (MQ=255)
                                                                  cTGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTg                        >  2:4332272/1‑94 (MQ=255)
                                                                   tGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGa                       >  2:6459326/1‑94 (MQ=255)
                                                                   tGAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGa                       >  1:7421689/1‑94 (MQ=255)
                                                                    gAAAGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGCGAg                      >  1:6012957/1‑94 (MQ=255)
                                                                     aaaGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGc                     >  1:2239029/1‑94 (MQ=255)
                                                                      aaGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCg                    <  2:7417313/94‑1 (MQ=255)
                                                                      aaGCACTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCg                    <  1:4717015/94‑1 (MQ=255)
                                                                       aGCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGAGTGCGCGt                   >  1:5688673/1‑94 (MQ=255)
                                                                        gCATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTc                  <  2:2109393/94‑1 (MQ=255)
                                                                         cATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCt                 <  2:10515562/94‑1 (MQ=255)
                                                                         cATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCt                 >  2:1217178/1‑94 (MQ=255)
                                                                         cATTGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTACAATGGCAGCGTGAGCGTCt                 >  1:6937638/1‑94 (MQ=255)
                                                                           ttGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGGGCGTCTgg               <  1:10577063/94‑1 (MQ=255)
                                                                            tGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTggc              >  2:2988840/1‑93 (MQ=255)
                                                                            tGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGt              >  2:704074/1‑94 (MQ=255)
                                                                            tGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGt              >  2:6104448/1‑94 (MQ=255)
                                                                            tGATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGt              <  2:6641581/94‑1 (MQ=255)
                                                                             gATCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTc             >  1:3907197/1‑94 (MQ=255)
                                                                              aTCGAGCAGCGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTcg            >  2:4966243/1‑94 (MQ=255)
                                                                                 aagcagcGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCAGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGa         >  1:439869/2‑94 (MQ=255)
                                                                                  agcagcGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGACATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGaa        <  1:6687250/94‑1 (MQ=255)
                                                                                  agcagcGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGaa        >  1:3535094/1‑94 (MQ=255)
                                                                                   gcagcGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAg       >  1:7948253/1‑94 (MQ=255)
                                                                                     agcGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCt     <  1:8677539/94‑1 (MQ=255)
                                                                                      gcGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTTGCGAAGTTg    <  1:5521261/94‑1 (MQ=255)
                                                                                      gcGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTg    <  1:3671931/94‑1 (MQ=255)
                                                                                       cGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAGGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTgg   >  2:7190538/1‑94 (MQ=255)
                                                                                       cGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTgg   <  1:5307800/94‑1 (MQ=255)
                                                                                       cGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTgg   >  1:7608993/1‑94 (MQ=255)
                                                                                       cGTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTgg   <  2:10041517/94‑1 (MQ=255)
                                                                                        gTCAGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGa  >  1:1249955/1‑94 (MQ=255)
                                                                                           |                                                                                          
ATTCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGA  >  NC_000913/3814690‑3814871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 426 total aligned reads displayed.
CTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  >  NC_000913/3814954‑3815140
                                                                                             |                                                                                             
CTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGC                                                                                               >  SRR4302545.4389017/1‑94 (MQ=60)
CTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGC                                                                                               <  SRR4302545.6158122/94‑1 (MQ=60)
 TGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCT                                                                                              >  SRR4302545.4229145/1‑94 (MQ=60)
 TGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCT                                                                                              >  SRR4302545.2691243/1‑94 (MQ=60)
 TGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCT                                                                                              >  SRR4302545.3237904/1‑94 (MQ=60)
 TGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCT                                                                                              >  SRR4302545.4730862/1‑94 (MQ=60)
   GATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCA                                                                                            >  SRR4302545.7124636/1‑94 (MQ=60)
     TCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAAC                                                                                          >  SRR4302545.1837673/1‑94 (MQ=60)
      CGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGGTGAACC                                                                                         >  SRR4302545.2963640/1‑94 (MQ=60)
      CGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACC                                                                                         >  SRR4302545.7705893/1‑94 (MQ=60)
      CGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACC                                                                                         <  SRR4302545.564527/94‑1 (MQ=60)
       GCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCG                                                                                        <  SRR4302545.5357501/94‑1 (MQ=60)
       GCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCG                                                                                        <  SRR4302545.6319605/94‑1 (MQ=60)
       GCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCG                                                                                        >  SRR4302545.10866954/1‑94 (MQ=60)
        CCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGC                                                                                       <  SRR4302545.7079296/94‑1 (MQ=60)
         CTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCG                                                                                      <  SRR4302545.3061692/94‑1 (MQ=60)
         CTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCG                                                                                      >  SRR4302545.59887/1‑94 (MQ=60)
         CTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCG                                                                                      >  SRR4302545.6486481/1‑94 (MQ=60)
           CGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAG                                                                                    >  SRR4302545.3103716/1‑94 (MQ=60)
           CGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAG                                                                                    >  SRR4302545.10189522/1‑94 (MQ=60)
            GAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCTAGT                                                                                   <  SRR4302545.9527378/94‑1 (MQ=60)
            GAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGT                                                                                   >  SRR4302545.933049/1‑94 (MQ=60)
            GAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGT                                                                                   >  SRR4302545.1529471/1‑94 (MQ=60)
              AGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCG                                                                                 >  SRR4302545.10008055/1‑94 (MQ=60)
              AGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCG                                                                                 >  SRR4302545.2874456/1‑94 (MQ=60)
              AGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCG                                                                                 >  SRR4302545.5552927/1‑94 (MQ=60)
              AGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCG                                                                                 <  SRR4302545.53699/94‑1 (MQ=60)
              AGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCG                                                                                 <  SRR4302545.10823252/94‑1 (MQ=60)
              AGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCG                                                                                 <  SRR4302545.1484543/94‑1 (MQ=60)
               GCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGA                                                                                <  SRR4302545.10641204/94‑1 (MQ=60)
                CGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAA                                                                               >  SRR4302545.7697893/1‑94 (MQ=60)
                CGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAA                                                                               >  SRR4302545.4602241/1‑94 (MQ=60)
                 GCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAAC                                                                              <  SRR4302545.1847840/94‑1 (MQ=60)
                 GCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAAC                                                                              <  SRR4302545.4594238/94‑1 (MQ=60)
                   ATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACAC                                                                            >  SRR4302545.594064/1‑94 (MQ=60)
                   ATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACAC                                                                            >  SRR4302545.6322666/1‑94 (MQ=60)
                    TGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACT                                                                           >  SRR4302545.693981/1‑94 (MQ=60)
                    TGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACT                                                                           <  SRR4302545.4106402/94‑1 (MQ=60)
                    TGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACT                                                                           >  SRR4302545.6357448/1‑94 (MQ=60)
                    TGTCAAAGAGACCTACAACATGCTGAAGAAAAAAGAAGCGGTTGGTCGTCGACTGGATTTTATGAAGCAGAAGCACAACCGCGAGTCGAACACT                                                                           <  SRR4302545.9293342/94‑1 (MQ=60)
                     GTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTC                                                                          >  SRR4302545.5092501/1‑94 (MQ=60)
                     GTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTC                                                                          <  SRR4302545.5893401/94‑1 (MQ=60)
                     GTCAAAGAGACCTAAAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTC                                                                          <  SRR4302545.2273140/94‑1 (MQ=60)
                      TCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCT                                                                         <  SRR4302545.416631/94‑1 (MQ=60)
                       CAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTT                                                                        <  SRR4302545.10702880/94‑1 (MQ=60)
                       CAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTT                                                                        <  SRR4302545.8405180/94‑1 (MQ=60)
                       CAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTT                                                                        >  SRR4302545.368091/1‑94 (MQ=60)
                        AAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTG                                                                       <  SRR4302545.6447317/94‑1 (MQ=60)
                        AAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTG                                                                       >  SRR4302545.4259388/1‑94 (MQ=60)
                        AAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTG                                                                       >  SRR4302545.5209124/1‑94 (MQ=60)
                          AGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCGACCGCGAGTCGAACACTCTTGCG                                                                     >  SRR4302545.5995683/1‑94 (MQ=60)
                          AGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCG                                                                     >  SRR4302545.5269914/1‑94 (MQ=60)
                          AGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCG                                                                     <  SRR4302545.4674733/94‑1 (MQ=60)
                          AGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCG                                                                     <  SRR4302545.78596/94‑1 (MQ=60)
                           GAGTCCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGT                                                                    >  SRR4302545.9651873/1‑94 (MQ=60)
                            AGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTC                                                                   <  SRR4302545.4961092/94‑1 (MQ=60)
                            AGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTC                                                                   >  SRR4302545.10377547/1‑94 (MQ=60)
                            AGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTC                                                                   <  SRR4302545.3002285/94‑1 (MQ=60)
                            AGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTC                                                                   >  SRR4302545.4322607/1‑94 (MQ=60)
                            AGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTC                                                                   <  SRR4302545.3018347/94‑1 (MQ=60)
                            AGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGCCGAACACTCTGGCGTC                                                                   >  SRR4302545.3840899/1‑94 (MQ=60)
                             GACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCG                                                                  <  SRR4302545.10445115/94‑1 (MQ=60)
                             GACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCG                                                                  >  SRR4302545.6584752/1‑94 (MQ=60)
                             GAACTACAACATTCTGAAGAAAAGAGAAGCGGTTGCACGTCGTCTGGATTTTATGATGCAGGAGCTCACCCGCGAGTCCACCACTCTTGCGTCG                                                                  <  SRR4302545.10931483/94‑1 (MQ=60)
                              ACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGA                                                                 >  SRR4302545.9254322/1‑94 (MQ=60)
                              ACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGA                                                                 <  SRR4302545.8444590/94‑1 (MQ=60)
                              ACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGA                                                                 >  SRR4302545.649265/1‑94 (MQ=60)
                               CCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAA                                                                >  SRR4302545.4829745/1‑94 (MQ=60)
                                CTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGGACACTCTTGCGTCGAAG                                                               >  SRR4302545.8937161/1‑94 (MQ=60)
                                 TACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGT                                                              <  SRR4302545.719883/94‑1 (MQ=60)
                                 TACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGC                                                              >  SRR4302545.1393838/1‑94 (MQ=60)
                                  ACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTC                                                             <  SRR4302545.608025/94‑1 (MQ=60)
                                  ACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTC                                                             <  SRR4302545.3934375/94‑1 (MQ=60)
                                   CAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCT                                                            <  SRR4302545.10035693/94‑1 (MQ=60)
                                   CAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCT                                                            <  SRR4302545.9013891/94‑1 (MQ=60)
                                   CAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCT                                                            >  SRR4302545.7943647/1‑94 (MQ=60)
                                   CAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCT                                                            >  SRR4302545.654166/1‑94 (MQ=60)
                                   CAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCT                                                            >  SRR4302545.7412305/1‑94 (MQ=60)
                                   CAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCT                                                            >  SRR4302545.4217460/1‑94 (MQ=60)
                                    AACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTA                                                           >  SRR4302545.966647/1‑94 (MQ=60)
                                    AACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTA                                                           <  SRR4302545.82079/94‑1 (MQ=60)
                                     ACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTAT                                                          >  SRR4302545.2548970/1‑94 (MQ=60)
                                     ACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTAT                                                          >  SRR4302545.7349681/1‑94 (MQ=60)
                                     ACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTAT                                                          <  SRR4302545.1131675/94‑1 (MQ=60)
                                     ACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTAT                                                          >  SRR4302545.8630630/1‑94 (MQ=60)
                                      CATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCACCCGCGAGTCGAACACTCTTGCGTCGAAGTCTATC                                                         <  SRR4302545.1375226/94‑1 (MQ=60)
                                      CATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATC                                                         >  SRR4302545.5877173/1‑94 (MQ=60)
                                      CATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATC                                                         >  SRR4302545.3772025/1‑94 (MQ=60)
                                      CATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATC                                                         <  SRR4302545.10786134/94‑1 (MQ=60)
                                      AATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGAGAGTCGAACACTCTTGCGTCGAAGTCTATA                                                         >  SRR4302545.9751397/1‑94 (MQ=60)
                                       ATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCA                                                        <  SRR4302545.10418256/94‑1 (MQ=60)
                                        TTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAA                                                       <  SRR4302545.4578135/94‑1 (MQ=60)
                                        TTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAA                                                       >  SRR4302545.1628240/1‑94 (MQ=60)
                                        TTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAA                                                       <  SRR4302545.1818688/94‑1 (MQ=60)
                                         TCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAAT                                                      >  SRR4302545.7011549/1‑94 (MQ=60)
                                          CTGAAGAAAAAAGAAGCTGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATG                                                     <  SRR4302545.8037538/94‑1 (MQ=60)
                                          CTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATC                                                     >  SRR4302545.8106635/1‑94 (MQ=60)
                                           TGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGC                                                    >  SRR4302545.7877033/1‑94 (MQ=60)
                                           TGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGC                                                    >  SRR4302545.2316252/1‑94 (MQ=60)
                                           TGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGCCTATCAATGC                                                    >  SRR4302545.6756292/1‑94 (MQ=60)
                                            GAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCC                                                   >  SRR4302545.5147305/1‑94 (MQ=60)
                                              AGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGCAGTCTATCAATGCCGA                                                 >  SRR4302545.4192206/1‑94 (MQ=60)
                                              AGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGA                                                 >  SRR4302545.3866056/1‑94 (MQ=60)
                                                AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAG                                               >  SRR4302545.3720216/1‑94 (MQ=60)
                                                 AAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGT                                              <  SRR4302545.2078375/94‑1 (MQ=60)
                                                 AAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGG                                              >  SRR4302545.5097384/1‑94 (MQ=60)
                                                  AAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTG                                             <  SRR4302545.8857065/94‑1 (MQ=60)
                                                   AAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGA                                            >  SRR4302545.1781367/1‑94 (MQ=60)
                                                   AAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGA                                            >  SRR4302545.9072973/1‑94 (MQ=60)
                                                    AAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGCAGTGAC                                           >  SRR4302545.5778477/1‑94 (MQ=60)
                                                    AAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGAC                                           >  SRR4302545.217158/1‑94 (MQ=60)
                                                    AAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGAC                                           >  SRR4302545.5412487/1‑94 (MQ=60)
                                                    AAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGAC                                           <  SRR4302545.3979943/94‑1 (MQ=60)
                                                     AGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACA                                          <  SRR4302545.10340253/94‑1 (MQ=60)
                                                      GAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAA                                         >  SRR4302545.2720103/1‑94 (MQ=60)
                                                       AAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAA                                        >  SRR4302545.8551136/1‑94 (MQ=60)
                                                       AAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAA                                        <  SRR4302545.1962051/94‑1 (MQ=60)
                                                         GCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACT                                      <  SRR4302545.4445798/94‑1 (MQ=60)
                                                         GCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACT                                      <  SRR4302545.5083220/94‑1 (MQ=60)
                                                         ACGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACT                                      <  SRR4302545.3947394/94‑1 (MQ=60)
                                                           GGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCC                                    >  SRR4302545.186276/1‑94 (MQ=60)
                                                           GGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCC                                    <  SRR4302545.2817164/94‑1 (MQ=60)
                                                           GGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCC                                    <  SRR4302545.5309386/94‑1 (MQ=60)
                                                           GGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCC                                    >  SRR4302545.173065/1‑94 (MQ=60)
                                                            GTTGGTCGTCGTCTGGATTTTATTATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTTACAAACTCCG                                   <  SRR4302545.7395920/94‑1 (MQ=60)
                                                            GTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCgc                                   >  SRR4302545.2100854/1‑92 (MQ=60)
                                                             TTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATAAATGCCGAAGTGACAAACTCCGC                                  >  SRR4302545.7711497/1‑94 (MQ=60)
                                                              TGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCC                                 >  SRR4302545.10531432/1‑94 (MQ=60)
                                                              TGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCC                                 >  SRR4302545.263710/1‑94 (MQ=60)
                                                               GGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGGCA                                >  SRR4302545.9061652/1‑94 (MQ=60)
                                                               GGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCA                                >  SRR4302545.8348776/1‑94 (MQ=60)
                                                               GGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCA                                >  SRR4302545.8040473/1‑94 (MQ=60)
                                                                GTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCGT                               >  SRR4302545.3044033/1‑94 (MQ=60)
                                                                GTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCAT                               <  SRR4302545.9119344/94‑1 (MQ=60)
                                                                GTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCAT                               <  SRR4302545.6635923/94‑1 (MQ=60)
                                                                  CGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCG                             >  SRR4302545.5496349/1‑94 (MQ=60)
                                                                  CGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCG                             >  SRR4302545.5689513/1‑94 (MQ=60)
                                                                   GTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGA                            <  SRR4302545.9390172/94‑1 (MQ=60)
                                                                   GTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGA                            >  SRR4302545.396698/1‑94 (MQ=60)
                                                                    TCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAG                           <  SRR4302545.10321746/94‑1 (MQ=60)
                                                                     CGTCTGGATTTTATGATGCAGGAGCTCCACCGCGAGTCGAACACTCTTGCGTCGAAGCCTATCAATGCCGAAGTGACAAACTCCGCCCTCGAGC                          >  SRR4302545.1609023/1‑94 (MQ=60)
                                                                     CGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGC                          <  SRR4302545.4643564/94‑1 (MQ=60)
                                                                      GTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCT                         >  SRR4302545.8067313/1‑94 (MQ=60)
                                                                      GTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCT                         <  SRR4302545.3358136/94‑1 (MQ=60)
                                                                      GTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGACATCGAGCT                         >  SRR4302545.6431975/1‑94 (MQ=60)
                                                                      GCCTGGATTTTATGATGCAAGAGCTCCACAGCGAGTCGAAAACTCTTGCGTAGAAGTCTATCAATGCCGAAGTGACAGACTCCCCCATTGAGCT                         >  SRR4302545.7329593/1‑94 (MQ=48)
                                                                       TCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGGCAAACTCCGCCATCGAGCTG                        >  SRR4302545.4435484/1‑94 (MQ=60)
                                                                       TCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTG                        <  SRR4302545.4787979/94‑1 (MQ=60)
                                                                       TCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTG                        >  SRR4302545.7128946/1‑94 (MQ=60)
                                                                       GCTGGATTTTATGATGGAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTAACAAACTCCGCCATCGAGCTG                        <  SRR4302545.8559507/94‑1 (MQ=60)
                                                                        CTGGATTTTATGATGCAGGAGTTAAAACGAGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACACACTACGCCATCGAGCTGA                       >  SRR4302545.6704075/1‑94 (MQ=60)
                                                                        CTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGA                       >  SRR4302545.3327012/1‑94 (MQ=60)
                                                                         TGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAA                      <  SRR4302545.3013230/94‑1 (MQ=60)
                                                                         TGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAA                      >  SRR4302545.2464412/1‑94 (MQ=60)
                                                                          GGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAA                     <  SRR4302545.3855425/94‑1 (MQ=60)
                                                                          GGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAA                     >  SRR4302545.1274433/1‑94 (MQ=60)
                                                                          GGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAA                     <  SRR4302545.6821360/94‑1 (MQ=60)
                                                                           GATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAG                    >  SRR4302545.6226566/1‑94 (MQ=60)
                                                                             TTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTG                  <  SRR4302545.1802999/94‑1 (MQ=60)
                                                                               TTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTT                <  SRR4302545.8333406/94‑1 (MQ=60)
                                                                               TTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTT                >  SRR4302545.5619534/1‑94 (MQ=60)
                                                                               TTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTT                <  SRR4302545.6880235/94‑1 (MQ=60)
                                                                                TATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTG               >  SRR4302545.9787982/1‑94 (MQ=60)
                                                                                TATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTG               <  SRR4302545.7245894/94‑1 (MQ=60)
                                                                                TATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTG               <  SRR4302545.4986752/94‑1 (MQ=60)
                                                                                 ATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGA              >  SRR4302545.6640003/1‑94 (MQ=60)
                                                                                  TGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGAT             >  SRR4302545.6004270/1‑94 (MQ=60)
                                                                                  TGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGAT             >  SRR4302545.3937861/1‑94 (MQ=60)
                                                                                  TGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGGCTATCAATGCCGAAGTGACCAACTCCGCCACCAAGCTGAAAGTGTTGAT             >  SRR4302545.7828739/1‑94 (MQ=60)
                                                                                   GATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATT            <  SRR4302545.3263291/94‑1 (MQ=60)
                                                                                   GATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATT            <  SRR4302545.10088881/94‑1 (MQ=60)
                                                                                   GATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATT            <  SRR4302545.7767860/94‑1 (MQ=60)
                                                                                   GATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATT            <  SRR4302545.5829444/94‑1 (MQ=60)
                                                                                    ATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTG           >  SRR4302545.5384643/1‑94 (MQ=60)
                                                                                     TGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGA          <  SRR4302545.5429100/94‑1 (MQ=60)
                                                                                       CATGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGGTGAGTGAGC        <  SRR4302545.9186739/94‑1 (MQ=60)
                                                                                       CAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGC        <  SRR4302545.3869819/94‑1 (MQ=60)
                                                                                       CAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGC        >  SRR4302545.7503480/1‑94 (MQ=60)
                                                                                       CAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGC        <  SRR4302545.959076/94‑1 (MQ=60)
                                                                                        TGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCA       >  SRR4302545.1251554/1‑94 (MQ=60)
                                                                                        AGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCA       <  SRR4302545.8419943/94‑1 (MQ=60)
                                                                                        AGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCA       >  SRR4302545.6029962/1‑94 (MQ=60)
                                                                                        AGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCA       <  SRR4302545.829412/94‑1 (MQ=60)
                                                                                        AGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCA       >  SRR4302545.3447427/1‑94 (MQ=60)
                                                                                        AGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCA       <  SRR4302545.667103/94‑1 (MQ=60)
                                                                                         GGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAG      <  SRR4302545.1314676/94‑1 (MQ=60)
                                                                                         GGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAG      <  SRR4302545.1877761/94‑1 (MQ=60)
                                                                                         GGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAG      <  SRR4302545.6679320/94‑1 (MQ=60)
                                                                                           AGTTCAACCGCGAGTCGAACACTCTTGGGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGAT    <  SRR4302545.7600226/94‑1 (MQ=60)
                                                                                            GCTCACCCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG   <  SRR4302545.7335399/94‑1 (MQ=60)
                                                                                            GCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG   <  SRR4302545.8889663/94‑1 (MQ=60)
                                                                                            GCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG   <  SRR4302545.506502/94‑1 (MQ=60)
                                                                                            GCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG   <  SRR4302545.2868252/94‑1 (MQ=60)
                                                                                            GCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG   >  SRR4302545.1599597/1‑94 (MQ=60)
                                                                                             CTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGG  >  SRR4302545.7906326/1‑94 (MQ=60)
                                                                                             CTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  >  SRR4302545.874580/1‑94 (MQ=60)
                                                                                             CTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  <  SRR4302545.3420863/94‑1 (MQ=60)
                                                                                             CTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  <  SRR4302545.9562575/94‑1 (MQ=60)
                                                                                             CTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  <  SRR4302545.8373981/94‑1 (MQ=60)
                                                                                             CTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  >  SRR4302545.3832907/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  >  NC_000913/3814954‑3815140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑