Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,047 T→C F249L (TTC→CTC)  yicC → putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,0470TC99.4% 2945.3 / ‑1.8 856F249L (TTC→CTC) yicCputative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (479/372);  total (481/375)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 877 total aligned reads displayed.

GGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGAT  >  NC_000913/3814956‑3815138
                                                                                            |                                                                                           
ggATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTc                                                                                           <  1:6500991/94‑1 (MQ=255)
   tCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAAc                                                                                        >  2:10425595/1‑94 (MQ=255)
   tCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAAc                                                                                        >  1:2502326/1‑94 (MQ=255)
    cGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAAcc                                                                                       <  2:6367308/94‑1 (MQ=255)
     gCCTCGAAGGGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACcg                                                                                      <  2:1632467/94‑1 (MQ=255)
     gCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACcg                                                                                      >  2:453396/1‑94 (MQ=255)
      ccTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACcgc                                                                                     <  1:6889801/94‑1 (MQ=255)
      ccTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACcgc                                                                                     >  2:7489409/1‑94 (MQ=255)
       cTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACcgcg                                                                                    >  1:6224844/1‑94 (MQ=255)
       cTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACcgcg                                                                                    <  2:1003927/94‑1 (MQ=255)
       cTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACcgcg                                                                                    >  2:9456915/1‑94 (MQ=255)
         gcaaGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAg                                                                                  <  2:7630085/92‑1 (MQ=255)
          gAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAg                                                                                  >  1:4966855/1‑94 (MQ=255)
          gAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGt                                                                                 >  2:7089318/1‑94 (MQ=255)
          gAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGt                                                                                 >  2:2088901/1‑94 (MQ=255)
            aGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCg                                                                               >  1:9718568/1‑94 (MQ=255)
            aGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCg                                                                               <  2:9792771/94‑1 (MQ=255)
            aGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCg                                                                               >  1:2806552/1‑94 (MQ=255)
               gcATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAac                                                                            <  2:4365053/94‑1 (MQ=255)
                 aTGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAacac                                                                          >  2:5491533/1‑94 (MQ=255)
                  tGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACAct                                                                         <  1:4006207/94‑1 (MQ=255)
                   gTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACActc                                                                        >  2:651911/1‑94 (MQ=255)
                     cAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCtt                                                                      <  2:2542503/94‑1 (MQ=255)
                      aaaGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTg                                                                     >  1:4155630/1‑94 (MQ=255)
                      aaaGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTg                                                                     >  1:4815893/1‑94 (MQ=255)
                        agagACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCg                                                                   >  1:5137971/1‑94 (MQ=255)
                        agagACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCg                                                                   <  2:1259453/94‑1 (MQ=255)
                        agagACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCg                                                                   <  1:76872/94‑1 (MQ=255)
                         gagaCCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGt                                                                  >  2:6922129/1‑94 (MQ=255)
                          agaCCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGCCGAACACTCTGGCGTc                                                                 >  1:3748574/1‑94 (MQ=255)
                            aCCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGa                                                               <  1:8207067/94‑1 (MQ=255)
                            aCCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGa                                                               >  1:635050/1‑94 (MQ=255)
                               tACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGt                                                            >  2:1386934/1‑94 (MQ=255)
                               tACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGt                                                            <  1:6586813/94‑1 (MQ=255)
                                acaacaTTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTc                                                           <  1:3838641/94‑1 (MQ=255)
                                  aacaTTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTa                                                         >  2:3917130/1‑94 (MQ=255)
                                  aacaTTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTa                                                         <  2:4852359/94‑1 (MQ=255)
                                  aacaTTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTa                                                         >  2:7763906/1‑94 (MQ=255)
                                     aTTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCa                                                      <  1:1414995/94‑1 (MQ=255)
                                      ttCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCaa                                                     <  1:1776087/94‑1 (MQ=255)
                                        cTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATg                                                   <  2:3430680/94‑1 (MQ=255)
                                        cTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATg                                                   <  2:2893439/94‑1 (MQ=255)
                                         tGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGc                                                  <  1:5442612/94‑1 (MQ=255)
                                          gaag‑aaAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGcc                                                 >  2:5743384/1‑94 (MQ=255)
                                            ag‑aaAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGa                                               <  1:2196411/94‑1 (MQ=255)
                                             g‑aaAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGaa                                              >  1:6005398/1‑94 (MQ=255)
                                               aaaaaaGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTACGTCGAAGTCTCTCAATGCCGAAg                                             >  1:10507521/1‑94 (MQ=255)
                                                aaaaaGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGt                                            <  2:8111160/94‑1 (MQ=255)
                                                aaaaaGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAAAACTCTTGCGTCGAAGTCTATCAATGCAGacgg                                            >  2:3032471/1‑91 (MQ=255)
                                                 aaaaGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTg                                           <  1:8607949/94‑1 (MQ=255)
                                                  aaaGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGa                                          >  1:8816988/1‑94 (MQ=255)
                                                  aaaGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGa                                          >  2:5925739/1‑94 (MQ=255)
                                                  aaaGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGa                                          <  2:4099591/94‑1 (MQ=255)
                                                   aagaagCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGAc                                         >  1:5276005/1‑94 (MQ=255)
                                                    agaagCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACa                                        >  2:9329944/1‑94 (MQ=255)
                                                    agaagCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACa                                        <  2:8818555/94‑1 (MQ=255)
                                                     gaagCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGTGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACaa                                       >  2:8612583/1‑94 (MQ=255)
                                                      aagCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACaaa                                      <  2:8966673/94‑1 (MQ=255)
                                                        gCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACt                                    <  1:4336388/94‑1 (MQ=255)
                                                        gCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACt                                    <  2:3695109/94‑1 (MQ=255)
                                                          ggTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTcc                                  <  2:2352967/94‑1 (MQ=255)
                                                           gTTGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCg                                 >  2:6227053/1‑94 (MQ=255)
                                                            ttGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGc                                >  1:7283450/1‑94 (MQ=255)
                                                            ttGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATAAATGCCGAAGTGACAAACTCCGc                                >  1:7499280/1‑94 (MQ=255)
                                                             tGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGcc                               <  2:8355263/94‑1 (MQ=255)
                                                             tGGTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGcc                               <  2:5118048/94‑1 (MQ=255)
                                                              ggTCGTCGTCTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCa                              >  2:10269715/1‑94 (MQ=255)
                                                               gtcgtcgtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACACACTCCGCCAt                             >  2:6750999/1‑94 (MQ=255)
                                                               gtcgtcgtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCAt                             >  1:1017798/1‑94 (MQ=255)
                                                                  gtcgtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGa                          <  2:5296217/94‑1 (MQ=255)
                                                                  gtcgtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAAATCCGCCATCGa                          >  2:2253618/1‑94 (MQ=255)
                                                                   tcgtcTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAg                         <  1:10024679/94‑1 (MQ=255)
                                                                   tcgtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAg                         <  2:4702116/94‑1 (MQ=255)
                                                                   tcgtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAg                         >  2:9669839/1‑94 (MQ=255)
                                                                    cgtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGc                        <  1:4527564/94‑1 (MQ=255)
                                                                     gtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCt                       >  1:7844875/1‑94 (MQ=255)
                                                                     gtcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCt                       >  2:1811738/1‑94 (MQ=255)
                                                                     gccTGGATTTTATGATGCAAGAGCTCCACAGCGAGTCGAAAACTCTTGCGTAGAAGTCTATCAATGCCGAAGTGACAGACTCCCCCATTGAGCt                       >  1:7131820/3‑94 (MQ=255)
                                                                      tcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTg                      >  2:3773686/1‑94 (MQ=255)
                                                                      tcTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTg                      >  2:7955880/1‑94 (MQ=255)
                                                                      cgtGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACCCTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTg                      <  2:9236107/92‑1 (MQ=255)
                                                                       cTGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTGTATCAATGCCGAAGTGACACACTCCGCCATCGAGCTGa                     >  1:5694210/1‑94 (MQ=255)
                                                                        tGGATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGaa                    <  1:3592082/94‑1 (MQ=255)
                                                                         ggATTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGaaa                   <  1:9074364/94‑1 (MQ=255)
                                                                           aTTTTATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAgt                 >  2:5048682/1‑94 (MQ=255)
                                                                              ttATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGtt              >  1:8106745/1‑94 (MQ=255)
                                                                              ttATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGtt              >  1:2948270/1‑94 (MQ=255)
                                                                              ttATGATGCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGtt              >  2:321724/1‑94 (MQ=255)
                                                                              ttATGATGCAGGAGCCCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGtt              <  1:1575001/94‑1 (MQ=255)
                                                                                atgatgCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGACGTGACAAACTCCGCCATCGAGCTGAAAGTGttga            >  2:269841/1‑94 (MQ=255)
                                                                                 tgatgCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGttgat           >  1:5846818/1‑94 (MQ=255)
                                                                                  gatgCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGttgatt          <  1:3185176/94‑1 (MQ=255)
                                                                                   atgCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGttgattg         <  2:10584487/94‑1 (MQ=255)
                                                                                    tgCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGttgattga        >  2:7271472/1‑94 (MQ=255)
                                                                                    tgCAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGttgattga        >  2:8543402/1‑94 (MQ=255)
                                                                                      cAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGc      <  1:938030/94‑1 (MQ=255)
                                                                                      cAGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGc      >  2:4948230/1‑94 (MQ=255)
                                                                                       tggAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCa     >  1:1223814/2‑94 (MQ=255)
                                                                                       aGGAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCa     <  2:1910478/94‑1 (MQ=255)
                                                                                        ggAGCTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAg    <  1:1833202/94‑1 (MQ=255)
                                                                                            |                                                                                           
GGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAG‑AAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGAT  >  NC_000913/3814956‑3815138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 462 total aligned reads displayed.
CGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  >  NC_000913/3851086‑3851272
                                                                                             |                                                                                             
CGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGC                                                                                               <  SRR4302545.9241094/94‑1 (MQ=60)
 GTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCA                                                                                              >  SRR4302545.2702594/1‑94 (MQ=60)
 GTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCA                                                                                              >  SRR4302545.10100440/1‑94 (MQ=60)
 GTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCA                                                                                              >  SRR4302545.9172604/1‑94 (MQ=60)
 GTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCA                                                                                              >  SRR4302545.1528823/1‑94 (MQ=60)
 GTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCA                                                                                              >  SRR4302545.11822/1‑94 (MQ=60)
 GTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCA                                                                                              >  SRR4302545.1107285/1‑94 (MQ=60)
  TTTCTCCTTGAGATACCCCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCAT                                                                                             <  SRR4302545.5840481/94‑1 (MQ=60)
   TTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATG                                                                                            >  SRR4302545.2218672/1‑94 (MQ=60)
   TTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATG                                                                                            >  SRR4302545.7930347/1‑94 (MQ=60)
    TCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGA                                                                                           <  SRR4302545.2000376/94‑1 (MQ=60)
    TCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGA                                                                                           <  SRR4302545.8186978/94‑1 (MQ=60)
     CTCCTTGAGATACCGCGTTCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACTGGAGACGCGCATGAA                                                                                          >  SRR4302545.9651886/1‑94 (MQ=60)
      TCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAAC                                                                                         >  SRR4302545.3726213/1‑94 (MQ=60)
       CCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACC                                                                                        <  SRR4302545.830843/94‑1 (MQ=60)
       CCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACC                                                                                        >  SRR4302545.9914865/1‑94 (MQ=60)
       CCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACC                                                                                        >  SRR4302545.66006/1‑94 (MQ=60)
       CCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACC                                                                                        >  SRR4302545.4903185/1‑94 (MQ=60)
       CCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACC                                                                                        >  SRR4302545.923211/1‑94 (MQ=60)
        CTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCT                                                                                       >  SRR4302545.8318071/1‑94 (MQ=60)
        CTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCT                                                                                       >  SRR4302545.4513957/1‑94 (MQ=60)
        CTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCT                                                                                       >  SRR4302545.10251444/1‑94 (MQ=60)
        CTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCT                                                                                       >  SRR4302545.6761658/1‑94 (MQ=60)
         TTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTG                                                                                      <  SRR4302545.3205807/94‑1 (MQ=60)
         TTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTG                                                                                      <  SRR4302545.6459659/94‑1 (MQ=60)
         TTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTG                                                                                      >  SRR4302545.10389169/1‑94 (MQ=60)
          TGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGG                                                                                     <  SRR4302545.10771356/94‑1 (MQ=60)
          TGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGG                                                                                     <  SRR4302545.1092859/94‑1 (MQ=60)
          TGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGG                                                                                     >  SRR4302545.7347979/1‑94 (MQ=60)
           GAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGG                                                                                    >  SRR4302545.2998916/1‑94 (MQ=60)
            AGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTG                                                                                   <  SRR4302545.3401239/94‑1 (MQ=60)
            AGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTG                                                                                   >  SRR4302545.4366625/1‑94 (MQ=60)
            AGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTG                                                                                   <  SRR4302545.887532/94‑1 (MQ=60)
            AGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTG                                                                                   <  SRR4302545.1063642/94‑1 (MQ=60)
             GATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGG                                                                                  >  SRR4302545.496369/1‑94 (MQ=60)
              ATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGA                                                                                 <  SRR4302545.8821401/94‑1 (MQ=60)
              ATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGA                                                                                 >  SRR4302545.80819/1‑94 (MQ=60)
               TACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGAT                                                                                >  SRR4302545.5480406/1‑94 (MQ=60)
               TACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGAT                                                                                <  SRR4302545.7875087/94‑1 (MQ=60)
               TACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGAT                                                                                <  SRR4302545.9571500/94‑1 (MQ=60)
               TACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGAT                                                                                >  SRR4302545.5879493/1‑94 (MQ=60)
               TACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGAT                                                                                >  SRR4302545.10363395/1‑94 (MQ=60)
               TACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGAT                                                                                >  SRR4302545.1499350/1‑94 (MQ=60)
                 CCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATAT                                                                              <  SRR4302545.2988326/94‑1 (MQ=60)
                 CCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATAT                                                                              >  SRR4302545.2248662/1‑94 (MQ=60)
                 CCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATAT                                                                              <  SRR4302545.4902192/94‑1 (MQ=60)
                  CGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATC                                                                             <  SRR4302545.4090898/94‑1 (MQ=60)
                  CGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATC                                                                             >  SRR4302545.2412410/1‑94 (MQ=60)
                  CGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATC                                                                             <  SRR4302545.9854108/94‑1 (MQ=60)
                   GCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCG                                                                            <  SRR4302545.7152558/94‑1 (MQ=60)
                   GCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCG                                                                            <  SRR4302545.4640040/94‑1 (MQ=60)
                   GCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCG                                                                            >  SRR4302545.4769170/1‑94 (MQ=60)
                    CGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGACCCTGGTGGATATCGC                                                                           >  SRR4302545.9392118/1‑94 (MQ=60)
                    CGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGC                                                                           >  SRR4302545.3597675/1‑94 (MQ=60)
                    CGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGC                                                                           >  SRR4302545.7965986/1‑94 (MQ=60)
                    CGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGC                                                                           >  SRR4302545.10021942/1‑94 (MQ=60)
                      TGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCA                                                                         >  SRR4302545.5721754/1‑94 (MQ=60)
                       GCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCAT                                                                        <  SRR4302545.6331751/94‑1 (MQ=60)
                       GCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCAT                                                                        >  SRR4302545.8189758/1‑94 (MQ=60)
                       GCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCAT                                                                        >  SRR4302545.1942636/1‑94 (MQ=60)
                        CACAACAGCTCGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATT                                                                       <  SRR4302545.9374902/94‑1 (MQ=60)
                        CAAAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATT                                                                       <  SRR4302545.6214602/94‑1 (MQ=60)
                         ACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTC                                                                      <  SRR4302545.2722346/94‑1 (MQ=60)
                         ACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTC                                                                      >  SRR4302545.10624484/1‑94 (MQ=60)
                         ACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTC                                                                      <  SRR4302545.3741857/94‑1 (MQ=60)
                          CAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCT                                                                     >  SRR4302545.1022689/1‑94 (MQ=60)
                          CAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCT                                                                     <  SRR4302545.5317431/94‑1 (MQ=60)
                          CAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCT                                                                     <  SRR4302545.8335565/94‑1 (MQ=60)
                          CAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCT                                                                     >  SRR4302545.8608153/1‑94 (MQ=60)
                           AACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTT                                                                    >  SRR4302545.6940867/1‑94 (MQ=60)
                           AACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTT                                                                    >  SRR4302545.5602581/1‑94 (MQ=60)
                           AACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTT                                                                    <  SRR4302545.4722456/94‑1 (MQ=60)
                            ACAGCTGGCAACAGGCAGGGGAAATGTACGTCAGCTGGAAGTGCTCCTGAACCACGGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTA                                                                   <  SRR4302545.1155136/94‑1 (MQ=60)
                            ACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTA                                                                   <  SRR4302545.2939591/94‑1 (MQ=60)
                             CAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTAT                                                                  >  SRR4302545.8057018/1‑94 (MQ=60)
                              AGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATC                                                                 >  SRR4302545.9267452/1‑94 (MQ=60)
                              AGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATC                                                                 <  SRR4302545.7463072/94‑1 (MQ=60)
                              AGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATC                                                                 >  SRR4302545.2095864/1‑94 (MQ=60)
                                CTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCT                                                               >  SRR4302545.28667/1‑94 (MQ=60)
                                CTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCT                                                               <  SRR4302545.1612510/94‑1 (MQ=60)
                                 TGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTC                                                              <  SRR4302545.3755365/94‑1 (MQ=60)
                                  GGCAGCAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCA                                                             >  SRR4302545.9092212/1‑94 (MQ=60)
                                  GGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCA                                                             <  SRR4302545.4126498/94‑1 (MQ=60)
                                   GCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAA                                                            <  SRR4302545.7823441/94‑1 (MQ=60)
                                   GCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAA                                                            >  SRR4302545.8661941/1‑94 (MQ=60)
                                    CAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAA                                                           >  SRR4302545.1074164/1‑94 (MQ=60)
                                     AACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAAC                                                          >  SRR4302545.10841414/1‑94 (MQ=60)
                                     AACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAAC                                                          >  SRR4302545.5656618/1‑94 (MQ=60)
                                     AACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAAC                                                          >  SRR4302545.10371722/1‑94 (MQ=60)
                                     AACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAAC                                                          <  SRR4302545.563116/94‑1 (MQ=60)
                                       CAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTC                                                        >  SRR4302545.4663508/1‑94 (MQ=60)
                                       CAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTC                                                        <  SRR4302545.8995978/94‑1 (MQ=60)
                                       CAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTC                                                        <  SRR4302545.6905887/94‑1 (MQ=60)
                                        AGGCAGGGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCA                                                       <  SRR4302545.6665501/94‑1 (MQ=60)
                                        AGGCAGCGTAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCA                                                       <  SRR4302545.1188157/94‑1 (MQ=60)
                                        AGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCA                                                       >  SRR4302545.377116/1‑94 (MQ=60)
                                         GGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCAT                                                      <  SRR4302545.6155179/94‑1 (MQ=60)
                                          GCAACGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGTTCATGAACCTGGTGGATATCGCCATTCTTATCCTCAATCTCATT                                                     <  SRR4302545.9019670/94‑1 (MQ=60)
                                            AGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGT                                                   >  SRR4302545.442479/1‑94 (MQ=60)
                                            AGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGT                                                   >  SRR4302545.6001759/1‑94 (MQ=60)
                                            AGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGT                                                   >  SRR4302545.3700373/1‑94 (MQ=60)
                                             GCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTT                                                  <  SRR4302545.2441861/94‑1 (MQ=60)
                                             GCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTT                                                  <  SRR4302545.10029816/94‑1 (MQ=60)
                                             GCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTT                                                  <  SRR4302545.9464944/94‑1 (MQ=60)
                                              CGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTG                                                 <  SRR4302545.4192011/94‑1 (MQ=60)
                                               GGAAAGGTACGTCAGCTGTCAGTGCTCCTGAACCACAGGAGATGCGTATGAACCTGGTGGATATCGCGATTCTTATCCTCAAACTCATGGTTGC                                                <  SRR4302545.1882087/94‑1 (MQ=60)
                                               GGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTC‑TATCCTCAAACTCATTGTTGCA                                               <  SRR4302545.2339166/94‑1 (MQ=60)
                                                GAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCA                                               <  SRR4302545.6296649/94‑1 (MQ=60)
                                                GAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCA                                               >  SRR4302545.5202346/1‑94 (MQ=60)
                                                GAAAGGTACGTCAGCTGACAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCA                                               >  SRR4302545.7939854/1‑94 (MQ=60)
                                                 AAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAG                                              >  SRR4302545.5323425/1‑94 (MQ=60)
                                                 AAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAG                                              >  SRR4302545.6745919/1‑94 (MQ=60)
                                                 AAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAG                                              >  SRR4302545.1424401/1‑94 (MQ=60)
                                                 AAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAG                                              <  SRR4302545.2358382/94‑1 (MQ=60)
                                                  AAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGC                                             >  SRR4302545.2885480/1‑94 (MQ=60)
                                                   AGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCA                                            <  SRR4302545.1095361/94‑1 (MQ=60)
                                                    GGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCAC                                           <  SRR4302545.10482892/94‑1 (MQ=60)
                                                    GGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCAC                                           <  SRR4302545.9531220/94‑1 (MQ=60)
                                                     GTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACT                                          >  SRR4302545.7271219/1‑94 (MQ=60)
                                                     GTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACT                                          <  SRR4302545.5806521/94‑1 (MQ=60)
                                                      TACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTG                                         <  SRR4302545.7562964/94‑1 (MQ=60)
                                                       ACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGC                                        <  SRR4302545.4841108/94‑1 (MQ=60)
                                                       ACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGC                                        <  SRR4302545.8421497/94‑1 (MQ=60)
                                                        CGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCA                                       >  SRR4302545.4602948/1‑94 (MQ=60)
                                                         GTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAA                                      <  SRR4302545.3363432/94‑1 (MQ=60)
                                                         GTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAA                                      >  SRR4302545.6046707/1‑94 (MQ=60)
                                                         GTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAA                                      >  SRR4302545.10869156/1‑94 (MQ=60)
                                                           CAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACT                                    >  SRR4302545.105741/1‑94 (MQ=60)
                                                           CAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACT                                    <  SRR4302545.4558117/94‑1 (MQ=60)
                                                            AGCTGGCAGTGCTCCTGAACCACTGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTG                                   <  SRR4302545.5981498/94‑1 (MQ=60)
                                                            AGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTG                                   <  SRR4302545.73803/94‑1 (MQ=60)
                                                            AGCTGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTG                                   <  SRR4302545.3188281/94‑1 (MQ=60)
                                                            AGCTGGCAGTGCGCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTG                                   >  SRR4302545.5929593/1‑94 (MQ=60)
                                                               TGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTT                                <  SRR4302545.3459389/94‑1 (MQ=60)
                                                               TGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTT                                >  SRR4302545.1456763/1‑94 (MQ=60)
                                                               TGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTT                                <  SRR4302545.4852071/94‑1 (MQ=60)
                                                               TGGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTT                                >  SRR4302545.3327902/1‑94 (MQ=60)
                                                                GGCAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTG                               >  SRR4302545.8489652/1‑94 (MQ=60)
                                                                  CAGTGCTCCTGAACCACAGGAGTCGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAT                             <  SRR4302545.2531463/94‑1 (MQ=60)
                                                                  CAGTGCTCCTGAACCACAGGAGACGCGCATGATCCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAT                             <  SRR4302545.7043551/94‑1 (MQ=60)
                                                                  CAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAT                             <  SRR4302545.3861765/94‑1 (MQ=60)
                                                                  CAGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAT                             <  SRR4302545.10145722/94‑1 (MQ=60)
                                                                   AGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATG                            >  SRR4302545.2877060/1‑94 (MQ=60)
                                                                   AGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATG                            >  SRR4302545.4237629/1‑94 (MQ=60)
                                                                   AGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATG                            >  SRR4302545.7791504/1‑94 (MQ=60)
                                                                   AGTGCTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATG                            <  SRR4302545.434444/94‑1 (MQ=60)
                                                                       CTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGT                        <  SRR4302545.8941895/94‑1 (MQ=60)
                                                                       CTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGT                        >  SRR4302545.8657398/1‑94 (MQ=60)
                                                                       CTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGT                        <  SRR4302545.9758506/94‑1 (MQ=60)
                                                                       CTCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGT                        >  SRR4302545.2076391/1‑94 (MQ=60)
                                                                        TCCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTT                       >  SRR4302545.9303733/1‑94 (MQ=60)
                                                                         CCTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTC                      >  SRR4302545.551433/1‑94 (MQ=60)
                                                                          CTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCT                     <  SRR4302545.7668176/94‑1 (MQ=60)
                                                                          CTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCT                     >  SRR4302545.1789210/1‑94 (MQ=60)
                                                                          CTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCT                     >  SRR4302545.985945/1‑94 (MQ=60)
                                                                          CTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCT                     >  SRR4302545.7121356/1‑94 (MQ=60)
                                                                          CTGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCT                     <  SRR4302545.3991160/94‑1 (MQ=60)
                                                                           TGAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTG                    <  SRR4302545.8954620/94‑1 (MQ=60)
                                                                            GAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGA                   >  SRR4302545.10093794/1‑94 (MQ=60)
                                                                            GAACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGA                   <  SRR4302545.2801290/94‑1 (MQ=60)
                                                                             AACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAA                  <  SRR4302545.8786217/94‑1 (MQ=60)
                                                                              ACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAA                 <  SRR4302545.6222851/94‑1 (MQ=60)
                                                                              ACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAA                 <  SRR4302545.2645336/94‑1 (MQ=60)
                                                                              ACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAA                 >  SRR4302545.7908848/1‑94 (MQ=60)
                                                                              ACCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAA                 <  SRR4302545.6222853/94‑1 (MQ=60)
                                                                               CCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAAT                <  SRR4302545.1315231/94‑1 (MQ=60)
                                                                               CCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAAT                <  SRR4302545.2195120/94‑1 (MQ=60)
                                                                               CCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAAT                <  SRR4302545.1297803/94‑1 (MQ=60)
                                                                               CCACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAAT                <  SRR4302545.844265/94‑1 (MQ=60)
                                                                                CACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATA               <  SRR4302545.1206803/94‑1 (MQ=60)
                                                                                CACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATA               <  SRR4302545.10705095/94‑1 (MQ=60)
                                                                                CACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATA               <  SRR4302545.8711909/94‑1 (MQ=60)
                                                                                CACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATA               >  SRR4302545.4061687/1‑94 (MQ=60)
                                                                                 ACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAC              <  SRR4302545.6135489/94‑1 (MQ=60)
                                                                                 ACAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAC              <  SRR4302545.10514723/94‑1 (MQ=60)
                                                                                  CAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACC             >  SRR4302545.4570545/1‑94 (MQ=60)
                                                                                  CAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACC             <  SRR4302545.3933136/94‑1 (MQ=60)
                                                                                  CAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACC             >  SRR4302545.2676431/1‑94 (MQ=60)
                                                                                  CAGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACC             >  SRR4302545.6089333/1‑94 (MQ=60)
                                                                                  CAGGAGACGCACATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGATCAGCAACTGCTTGATGCTGTTCTGAAATACC             <  SRR4302545.1654305/94‑1 (MQ=60)
                                                                                   AGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCT            <  SRR4302545.262331/94‑1 (MQ=60)
                                                                                   AGGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCT            >  SRR4302545.6830823/1‑94 (MQ=60)
                                                                                    GGAGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTG           >  SRR4302545.6344992/1‑94 (MQ=60)
                                                                                      AGACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAA         >  SRR4302545.8904239/1‑94 (MQ=60)
                                                                                       GACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAG        <  SRR4302545.9723516/94‑1 (MQ=60)
                                                                                       GACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAG        <  SRR4302545.2085449/94‑1 (MQ=60)
                                                                                        ACGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGT       >  SRR4302545.3132319/1‑94 (MQ=60)
                                                                                         CGCGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTA      <  SRR4302545.10308560/94‑1 (MQ=60)
                                                                                           CGCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAAT    <  SRR4302545.6550421/94‑1 (MQ=60)
                                                                                            GCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATT   >  SRR4302545.3260545/1‑94 (MQ=60)
                                                                                            GCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATT   >  SRR4302545.2494344/1‑94 (MQ=60)
                                                                                            GCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATT   <  SRR4302545.10899606/94‑1 (MQ=60)
                                                                                            GCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATT   <  SRR4302545.3560207/94‑1 (MQ=60)
                                                                                            GCATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATT   >  SRR4302545.5843053/1‑94 (MQ=60)
                                                                                             CATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  >  SRR4302545.8204096/1‑94 (MQ=60)
                                                                                             CATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  <  SRR4302545.127484/94‑1 (MQ=60)
                                                                                             CATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  <  SRR4302545.7917125/94‑1 (MQ=60)
                                                                                             CATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  <  SRR4302545.2465452/94‑1 (MQ=60)
                                                                                             CATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  >  SRR4302545.10667198/1‑94 (MQ=60)
                                                                                             CATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACCGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  >  SRR4302545.763462/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTC  >  NC_000913/3851086‑3851272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑