Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,554,113 Δ1 bp coding (372/396 nt) yhgKJ ← RNA phosphage cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,554,1130C.98.2% 1525.6 / 14.3 333coding (372/396 nt)yhgKJRNA phosphage cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base . (154/172);  minor base G (5/1);  total (159/174)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 381 total aligned reads displayed.

GCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCT  >  NC_000913/3554023‑3554202
                                                                                           |                                                                                          
 cGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                                                     <  2:7407989/96‑2 (MQ=255)
  gTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccg                                                                                    >  1:2453598/1‑94 (MQ=255)
    ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgct                                                                                  >  2:8366213/1‑92 (MQ=255)
      tttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCa                                                                                 <  1:12150735/96‑1 (MQ=255)
         gCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCATGCGGCGCCGACGGGGTATCGGGGCCCC‑CCCTCACtt                                                                              >  1:4756902/1‑96 (MQ=255)
          cggatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGACGGCGGCGACGGTGTATCGGT‑GCCGCCGCTCACTTc                                                                             >  1:10259785/3‑96 (MQ=255)
          cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTc                                                                             >  2:11755810/1‑96 (MQ=255)
          cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTc                                                                             <  1:2074412/96‑1 (MQ=255)
           tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGGGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCa                                                                            >  2:12039617/1‑96 (MQ=255)
            gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCaa                                                                           >  1:5826639/1‑96 (MQ=255)
             atgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                                                          <  2:8060489/96‑1 (MQ=255)
             atgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                                                          >  2:2023515/1‑96 (MQ=255)
             atgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCCCTTCAac                                                                          >  1:3297271/1‑96 (MQ=255)
               gaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAacac                                                                        <  2:2608209/96‑1 (MQ=255)
               gaATTCCTATTTTCGCAAGCAGCGGCTCCAGCACCCGGCGGATAAAAGCCGCAGGCGGCGCCGACGGCTTATCGGT‑GCCGCCGCTCACTTCAacac                                                                        <  1:8743162/96‑1 (MQ=255)
                 aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGTCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgc                                                                      >  2:12232100/1‑96 (MQ=255)
                 aTTCCTATTTTCGCCAGCAGCGCCTCGAGCACCCGGCGCATAAAATCCGCAGGCGGCGCCGCCGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgc                                                                      <  1:12450072/96‑1 (MQ=255)
                  ttCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgcg                                                                     <  2:10818893/96‑1 (MQ=255)
                     cTATTTTCGCCAGCAGCGGCTCCAGCTCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                                                  <  1:4846700/96‑1 (MQ=255)
                      tATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAgg                                                                 <  2:959315/96‑1 (MQ=255)
                      tATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGG‑GGCCGCCGCTCACTTCAACACGCGAAgg                                                                 >  1:7408961/1‑96 (MQ=255)
                       aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                                                >  1:8106563/1‑96 (MQ=255)
                       aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                                                <  2:5899062/96‑1 (MQ=255)
                       aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                                                <  1:12908208/96‑1 (MQ=255)
                        ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTc                                                               >  2:3186908/1‑96 (MQ=255)
                           tCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                            >  1:1523444/1‑96 (MQ=255)
                           tCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGCCCAt                                                            >  1:3488504/1‑96 (MQ=255)
                            cGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGGCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGGCCGTc                                                           >  1:660432/1‑96 (MQ=255)
                             gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                                                          >  1:4226628/1‑96 (MQ=255)
                             gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                                                          >  1:6220569/1‑96 (MQ=255)
                             gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                                                          >  2:6977185/1‑96 (MQ=255)
                              ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                         <  2:1632156/96‑1 (MQ=255)
                              ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                         >  1:13181904/1‑96 (MQ=255)
                               cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGc                                                        <  2:11112095/96‑1 (MQ=255)
                               cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGc                                                        >  2:7937651/1‑96 (MQ=255)
                                agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCa                                                       >  2:11509288/1‑96 (MQ=255)
                                   agcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAc                                                    >  1:1075131/1‑96 (MQ=255)
                                    gcGGCTCCAGCCCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                   <  1:6487122/96‑1 (MQ=255)
                                    gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                   <  1:7160921/96‑1 (MQ=255)
                                    gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                   <  1:11600048/96‑1 (MQ=255)
                                    gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGGCCATCGGCACAcc                                                   >  1:9056212/1‑96 (MQ=255)
                                     cGGATCCAGCACCCGGCGGATAAAAACCGCAGGCGGCGCAGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                                                  <  1:1229798/96‑1 (MQ=255)
                                      ggCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcccc                                                 <  1:11076942/96‑3 (MQ=255)
                                      ggCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcac                                                 >  1:1690651/1‑96 (MQ=255)
                                      ggCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcac                                                 >  2:5535685/1‑96 (MQ=255)
                                       gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcaca                                                >  2:5073448/1‑96 (MQ=255)
                                       gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcaca                                                >  2:11484595/1‑96 (MQ=255)
                                         tCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                                              <  2:7806313/96‑1 (MQ=255)
                                         tCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                                              <  2:5586098/96‑1 (MQ=255)
                                         tCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGCCCATCGGCAAACCACAgc                                              >  1:4645251/1‑96 (MQ=255)
                                         tCCAACACCCGGCGGATACAATCCGCAGGCGGCGCCGACAGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                                              >  1:10137656/1‑96 (MQ=255)
                                          ccAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcg                                             <  2:13515860/96‑1 (MQ=255)
                                             gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg                                          <  2:12723398/96‑1 (MQ=255)
                                             gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg                                          <  1:10364858/96‑1 (MQ=255)
                                             gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCCCgg                                          <  2:10109740/96‑1 (MQ=255)
                                               gcccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgc                                        <  1:852849/95‑1 (MQ=255)
                                                cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAACACAGCGCGGgca                                       >  2:626345/1‑96 (MQ=255)
                                                  cggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTTAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagc                                     <  2:14028606/96‑1 (MQ=255)
                                                   ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca                                    <  2:13859306/96‑1 (MQ=255)
                                                   ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca                                    <  2:9211370/96‑1 (MQ=255)
                                                   ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca                                    >  2:9785578/1‑96 (MQ=255)
                                                   ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca                                    <  1:9667558/96‑1 (MQ=255)
                                                    gcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAc                                   >  1:13126183/1‑96 (MQ=255)
                                                     cggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAcc                                  >  1:7698271/1‑96 (MQ=255)
                                                     cggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAcc                                  <  2:2342112/96‑1 (MQ=255)
                                                      ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg                                 >  1:4671273/1‑96 (MQ=255)
                                                      ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg                                 <  2:10441545/96‑1 (MQ=255)
                                                         tAAAGTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGttt                              <  1:12950587/96‑1 (MQ=255)
                                                         tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGttt                              <  2:10255217/96‑1 (MQ=255)
                                                         tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGttt                              >  2:861448/1‑96 (MQ=255)
                                                           aaaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgc                            <  2:358652/96‑1 (MQ=255)
                                                               ccgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcagca                        >  1:11907634/5‑96 (MQ=255)
                                                               ccgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCACCACCGTTTgcagca                        >  2:4900461/5‑96 (MQ=255)
                                                                cgccggcggcGCCGACGGGTTATCGGT‑GCCGCCACTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAc                       <  1:2640565/92‑1 (MQ=255)
                                                                  caGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCa                     <  2:13234407/95‑1 (MQ=255)
                                                                   aGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCCCCAg                    <  2:1334343/96‑1 (MQ=255)
                                                                   aGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAg                    >  1:3025059/1‑96 (MQ=255)
                                                                   aGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGCCCATCGCCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAg                    >  1:12502463/1‑96 (MQ=255)
                                                                    ggcggcGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCTAACCACAGCGCGGGCAGCACCGTTTGCagcagcagc                   >  1:12696742/1‑96 (MQ=255)
                                                                      cggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCgc                 <  1:11374964/96‑2 (MQ=255)
                                                                      cggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGt                 >  2:284137/1‑96 (MQ=255)
                                                                      cggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGt                 >  1:7134002/1‑96 (MQ=255)
                                                                      cggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGt                 <  2:7169867/96‑1 (MQ=255)
                                                                       ggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTa                >  2:4745745/1‑96 (MQ=255)
                                                                        gcgcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTAc               >  2:5280762/1‑96 (MQ=255)
                                                                          gcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACaa             >  1:11530888/1‑96 (MQ=255)
                                                                          gcCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGTCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACaa             >  1:1583758/1‑96 (MQ=255)
                                                                            cGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACt           <  1:9363947/96‑1 (MQ=255)
                                                                            cGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACt           <  1:6708801/96‑1 (MQ=255)
                                                                             gACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACtt          >  2:4641899/1‑96 (MQ=255)
                                                                              aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTc         >  1:5150030/1‑96 (MQ=255)
                                                                              aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTc         <  2:5477323/96‑1 (MQ=255)
                                                                              aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTc         >  2:1760009/1‑96 (MQ=255)
                                                                               cGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc        <  1:13609407/96‑1 (MQ=255)
                                                                                gggTTATCTGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAAATTcca       >  1:385529/1‑95 (MQ=255)
                                                                                gggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCg       >  1:6999745/1‑96 (MQ=255)
                                                                                gggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCg       <  1:10012440/96‑1 (MQ=255)
                                                                                 ggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCgg      >  2:8786360/1‑96 (MQ=255)
                                                                                  gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGgc     <  2:3714954/96‑1 (MQ=255)
                                                                                    tATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGgcgc   >  2:4032178/1‑96 (MQ=255)
                                                                                           |                                                                                          
GCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCT  >  NC_000913/3554023‑3554202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 229 total aligned reads displayed.
GGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCG‑‑GT‑‑CGCCG‑C‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCG  >  NC_000913/3554014‑3554206
                                                                                                        |                                                                                               
GGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATGCGCAGGCAGCGCCGACCGGTTATCG‑‑NN‑‑N                                                                                                 >  SRR4302518.10027683/1‑101 (MQ=60)
 GCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GN‑‑NN                                                                                                >  SRR4302518.12582902/1‑101 (MQ=60)
  CGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑NNN                                                                                               >  SRR4302518.10357459/1‑101 (MQ=60)
  CGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCC                                                                                              <  SRR4302518.54617/101‑1 (MQ=60)
   GTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCG                                                                                             <  SRR4302518.6820822/101‑1 (MQ=60)
   GTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCG                                                                                             >  SRR4302518.5756187/1‑101 (MQ=60)
     nnnagttgggttttcttttaatTTCCTGTTTTCGCCAGCCGCGGTTCCAGCACCCGGCGGAAAAAATCCGCGGGCGGCGCCGACGGGTTATCG‑‑GTGCCGCC                                                                                              <  SRR4302518.11629292/79‑1 (MQ=39)
     NNNAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GTGCCGCC                                                                                              <  SRR4302518.13787998/101‑1 (MQ=60)
     AACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GTGCCGCC                                                                                              <  SRR4302518.4940527/101‑1 (MQ=60)
     AACAGAGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GTGCCGCC                                                                                              <  SRR4302518.2182768/101‑1 (MQ=60)
      NNNGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GTGCCGCCG                                                                                             <  SRR4302518.12921192/101‑1 (MQ=60)
      ACAGCGTGCTTTGCTGATGACTTCCTATTTTCGCCAGCAGGGTCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GTGCCGCCG                                                                                             <  SRR4302518.4049987/101‑1 (MQ=60)
       CAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GTGCCGCCG‑C                                                                                           >  SRR4302518.4262811/1‑101 (MQ=60)
          CGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCA                                                                                     >  SRR4302518.6848321/1‑101 (MQ=60)
           GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCAC                                                                                    >  SRR4302518.2572034/1‑101 (MQ=60)
            TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTAGCG‑‑GT‑‑‑GCCGCC‑GCTCACT                                                                                   >  SRR4302518.4100150/1‑101 (MQ=60)
            NNNTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACT                                                                                   <  SRR4302518.7528026/101‑1 (MQ=60)
              NNNTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTC                                                                                 <  SRR4302518.12976561/101‑1 (MQ=60)
              GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTC                                                                                 <  SRR4302518.2171159/101‑1 (MQ=60)
               nnnggttcTGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCC‑‑GT‑‑‑GCCGCC‑GCTCACTTCA                                                                                <  SRR4302518.11994118/93‑1 (MQ=60)
                NNNCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCCA                                                                               <  SRR4302518.11097553/101‑1 (MQ=60)
                 TGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAAC                                                                              >  SRR4302518.485413/1‑101 (MQ=60)
                  NNNGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACA                                                                             <  SRR4302518.8316882/101‑1 (MQ=60)
                  GCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCCCACTTCAACA                                                                             >  SRR4302518.5017115/1‑101 (MQ=60)
                  GCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCANNN                                                                             >  SRR4302518.7546432/1‑101 (MQ=60)
                   NNNATGAATTCCTATTTTCGCAAGCAGCGGCTCCAGCACCCGGCGGATAAAAGCCGCAGGCGGCGCCGACGGCTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACAC                                                                            <  SRR4302518.9167995/101‑1 (MQ=60)
                   CGGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGACGGCGGCGACGGTGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAANNN                                                                            >  SRR4302518.10744018/1‑101 (MQ=60)
                    TGATGCATTCCTATTTTCGCCAGCAGCGGCTCCAGCCCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACG                                                                           <  SRR4302518.5339616/101‑1 (MQ=60)
                    TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACG                                                                           <  SRR4302518.485434/101‑1 (MQ=60)
                    NNNTGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACG                                                                           <  SRR4302518.13741248/101‑1 (MQ=60)
                     NNNGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGC                                                                          <  SRR4302518.10924978/101‑1 (MQ=60)
                     NNNGAATTCCTATTTTCGCCAGCAGCGCCTCGAGCACCCGGCGCATAAAATCCGCAGGCGGCGCCGCCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGC                                                                          <  SRR4302518.13036057/101‑1 (MQ=60)
                     GGTGAATTCCTGTTTTCGCCAGCAGGGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGC                                                                          <  SRR4302518.6660879/101‑1 (MQ=60)
                     GATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACANNN                                                                          >  SRR4302518.7838555/1‑101 (MQ=60)
                     GATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGC                                                                          >  SRR4302518.6121796/1‑101 (MQ=60)
                     GATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACANNN                                                                          >  SRR4302518.10830881/1‑101 (MQ=60)
                      NNNAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCG                                                                         <  SRR4302518.12573307/101‑1 (MQ=60)
                      NNNAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCG                                                                         <  SRR4302518.9893487/101‑1 (MQ=60)
                      ATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACNNN                                                                         >  SRR4302518.13165480/1‑101 (MQ=60)
                      ATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACNNN                                                                         >  SRR4302518.10431183/1‑101 (MQ=60)
                      ATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCCCTTCAACACGCG                                                                         >  SRR4302518.3450616/1‑101 (MQ=60)
                       TGAATTCCAATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGNNN                                                                        >  SRR4302518.13543832/1‑101 (MQ=60)
                        GAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCNNN                                                                       >  SRR4302518.7428988/1‑101 (MQ=60)
                        GAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAA                                                                       >  SRR4302518.3255111/1‑101 (MQ=60)
                        GAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAA                                                                       >  SRR4302518.6708943/1‑101 (MQ=60)
                         NNNTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAG                                                                      <  SRR4302518.10533981/101‑1 (MQ=60)
                         AATTCCTATTTTCGCCAGCAGCGGCTCCAGCTCCGGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAG                                                                      <  SRR4302518.5099987/101‑1 (MQ=60)
                         AATTCCTATTTTCGCCAGCAGCGCCTCAAGCGCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGCTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAG                                                                      <  SRR4302518.733839/101‑1 (MQ=60)
                         AATTCCCATTTTCGCCAGCAGCGACTCCAGCCCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAG                                                                      <  SRR4302518.6727412/101‑1 (MQ=60)
                          ATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAATG                                                                     <  SRR4302518.7148164/101‑1 (MQ=60)
                           TTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑GGCGC‑C‑GCTCACTTCAACACGCGAANNN                                                                    >  SRR4302518.8018179/1‑101 (MQ=60)
                           NNNCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGT                                                                    <  SRR4302518.13507209/101‑1 (MQ=60)
                           NNNCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGT                                                                    <  SRR4302518.13363252/101‑1 (MQ=60)
                             CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCC                                                                  <  SRR4302518.2469676/101‑1 (MQ=60)
                             CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCC                                                                  >  SRR4302518.6130092/1‑101 (MQ=60)
                              CTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCA                                                                 >  SRR4302518.7295641/1‑101 (MQ=60)
                              CTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCA                                                                 >  SRR4302518.7191694/1‑101 (MQ=60)
                               nnnttaCGCCAGCAGCGGCTCCAGCTCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCGT                                                                <  SRR4302518.9811203/95‑1 (MQ=60)
                               TATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACcgcaagggccca                                                                >  SRR4302518.6860534/1‑89 (MQ=60)
                               TATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑G‑‑‑GGCCGCC‑GCTCACTTCAACACGCGAAGGTCNNN                                                                >  SRR4302518.7792038/1‑101 (MQ=60)
                                ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGCCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATC                                                               <  SRR4302518.2638742/101‑1 (MQ=60)
                                ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCNNN                                                               >  SRR4302518.8509205/1‑101 (MQ=60)
                                ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATC                                                               >  SRR4302518.3230055/1‑101 (MQ=60)
                                 NNNTCGCCATCTGCGGCTCCAGCACCCTGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCT‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATTG                                                              <  SRR4302518.8539670/101‑1 (MQ=60)
                                 NNNTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCG                                                              <  SRR4302518.9643392/101‑1 (MQ=60)
                                  TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATNNN                                                             >  SRR4302518.12114323/1‑101 (MQ=60)
                                   TTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GGTCACTTCAACACGCGAAGGTCCATCNNN                                                            >  SRR4302518.13088449/1‑101 (MQ=60)
                                   TTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCNNN                                                            >  SRR4302518.9700056/1‑101 (MQ=60)
                                   NNNGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGC                                                            <  SRR4302518.14709994/101‑1 (MQ=60)
                                    TCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGCCCATTGGCA                                                           >  SRR4302518.3650782/1‑101 (MQ=60)
                                     CGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGGCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGGCCGTCGGGAA                                                          >  SRR4302518.686046/1‑101 (MQ=60)
                                      NNNAGCAGCGGCTCCAGCCCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCATA                                                         <  SRR4302518.12352812/101‑1 (MQ=60)
                                      GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACTGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCGCTTCGACACTCGAAGGTCCATCcgcnnn                                                         >  SRR4302518.8669268/1‑95 (MQ=48)
                                      GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAA                                                         >  SRR4302518.4433648/1‑101 (MQ=60)
                                      GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAA                                                         >  SRR4302518.6537792/1‑101 (MQ=60)
                                       CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGACGGTCCAGCGGCAAAC                                                        >  SRR4302518.3573941/1‑101 (MQ=60)
                                       CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCANNN                                                        >  SRR4302518.13790341/1‑101 (MQ=60)
                                        NNNCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                       <  SRR4302518.12146382/101‑1 (MQ=60)
                                        NNNCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                       <  SRR4302518.7537220/101‑1 (MQ=60)
                                        CAGCCGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTCATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                       <  SRR4302518.293142/101‑1 (MQ=60)
                                        CAGCAGCGGCTCCAGCCCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                       <  SRR4302518.6819268/101‑1 (MQ=60)
                                        CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAANNN                                                       >  SRR4302518.9148443/1‑101 (MQ=60)
                                        CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGCGGTATCT‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACCCGAAGGGCCATCGGCAAACC                                                       >  SRR4302518.6897535/1‑101 (MQ=58)
                                         nnntgcgtcgCCAGCCCCCGGCGGATAAAATCCGCCGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCAGCC‑GCTCACTTCAACACGCGAAGGTACATCGGCAAACCA                                                      <  SRR4302518.14512283/91‑1 (MQ=52)
                                         NNNAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCA                                                      <  SRR4302518.10209022/101‑1 (MQ=60)
                                         AGCCGCGGCTCCAGCCCCCGGCGGATAAACTCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCA                                                      <  SRR4302518.405248/101‑1 (MQ=60)
                                         AGCAGCGGCTCCAGCACCCGGTGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGT‑‑CGC‑‑‑C‑GCTCACTTCAACACGCGAAGGTCCATCGGTAAANNN                                                      >  SRR4302518.10902353/1‑101 (MQ=60)
                                         AGCAGCGGATCCAGCACCCGGCGGATAAAAACCGCAGGCGGCGCAGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCA                                                      <  SRR4302518.1279779/101‑1 (MQ=60)
                                          NNNGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCCC                                                     <  SRR4302518.11595118/101‑1 (MQ=60)
                                          GCCGCGGCCCCAGCACCCGGCGGATAAAACCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                                                     <  SRR4302518.4671592/101‑1 (MQ=60)
                                          GCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                                                     >  SRR4302518.4573247/1‑101 (MQ=60)
                                           nnncgcCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                                                    <  SRR4302518.14771344/95‑1 (MQ=60)
                                           CAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCNNN                                                    >  SRR4302518.8114008/1‑101 (MQ=60)
                                           CAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                                                    <  SRR4302518.3866422/101‑1 (MQ=60)
                                           CAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                                                    <  SRR4302518.1658145/101‑1 (MQ=60)
                                            NNNGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                                                   <  SRR4302518.8556749/101‑1 (MQ=60)
                                            GGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCANNN                                                   >  SRR4302518.11866896/1‑101 (MQ=60)
                                            GGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCANNN                                                   >  SRR4302518.9305536/1‑101 (MQ=60)
                                            AGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCANNN                                                   >  SRR4302518.13053651/1‑101 (MQ=60)
                                            AGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                                                   >  SRR4302518.1117547/1‑101 (MQ=60)
                                            AGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACACGAAGGACCATCGGCAAACCANNN                                                   >  SRR4302518.8602762/1‑101 (MQ=60)
                                            AGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGGAGGTCCATCGGGAAACCACAG                                                   >  SRR4302518.2794102/1‑101 (MQ=60)
                                            AGCGGCTCCAGCACCCGGCGGATAAAATCCGAAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCANNN                                                   >  SRR4302518.12752989/1‑101 (MQ=60)
                                             GCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGGCCATCGGCACACCACNNN                                                  >  SRR4302518.9493791/1‑101 (MQ=60)
                                             GCGGCTCCAGCACCCGGCGGATAAAAGCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                  <  SRR4302518.1639745/101‑1 (MQ=60)
                                              NNNCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                                                 <  SRR4302518.14560403/101‑1 (MQ=60)
                                              CGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACANNN                                                 >  SRR4302518.11897281/1‑101 (MQ=60)
                                               NNNTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGC                                                <  SRR4302518.11417416/101‑1 (MQ=60)
                                               GGCTCCAGGACCCGGCGGATAAAATCCGCAGGAGGCGCCGACGGGGTAACG‑‑GT‑‑‑GCCGCC‑GCTCACCTCAACACGCGAACGTCCATCGTCAAACCAAAGCGC                                                >  SRR4302518.5129877/1‑101 (MQ=28)
                                               GGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATGGGCAAACCACAGNNN                                                >  SRR4302518.12500635/1‑101 (MQ=60)
                                               GGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGNNN                                                >  SRR4302518.13857043/1‑101 (MQ=60)
                                               GGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGC                                                >  SRR4302518.1765313/1‑101 (MQ=60)
                                               GGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAAGTCCATCGGCAAACCACAGCGC                                                >  SRR4302518.1933887/1‑101 (MQ=60)
                                                GCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCNNN                                               >  SRR4302518.10800772/1‑101 (MQ=60)
                                                 NNNCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                                              <  SRR4302518.10852021/101‑1 (MQ=60)
                                                 CGCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                                              <  SRR4302518.3093230/101‑1 (MQ=60)
                                                  nnnacccCCCGGCGGATAAAAGCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑GCCTC‑C‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                                             <  SRR4302518.11483802/94‑1 (MQ=60)
                                                  TCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGCCCATCGGCAAACCACAGCGCGGG                                             >  SRR4302518.4889810/1‑101 (MQ=60)
                                                  TCCAACACCCGGCGGATACAATCCGCAGGCGGCGCCGACAGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCNNN                                             >  SRR4302518.10616790/1‑101 (MQ=60)
                                                   NNNGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                                            <  SRR4302518.7670119/101‑1 (MQ=60)
                                                   CCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                                            >  SRR4302518.2763183/1‑101 (MQ=60)
                                                   CCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                                            <  SRR4302518.7168000/101‑1 (MQ=60)
                                                    nnnccccggcgaacaccacgcgG‑AGGCGGCGCGGCCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACCCGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAG                                          <  SRR4302518.9015618/79‑1 (MQ=60)
                                                    GAGCACCCGGCGGGTAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGtnnn                                           >  SRR4302518.11956712/1‑97 (MQ=60)
                                                    CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCTGCAAACCACAGCGCGGGCA                                           >  SRR4302518.5699427/1‑101 (MQ=60)
                                                     agcgccctgaggatcaacgccgcAGGCGGCGGCGACGGGTTACCG‑‑GT‑‑‑GCCGCC‑GCTCCCTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAG                                          <  SRR4302518.5363961/78‑1 (MQ=60)
                                                     CGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAG                                          <  SRR4302518.1621089/101‑1 (MQ=60)
                                                     AGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACGACAGCGCGGGNNN                                          >  SRR4302518.13691156/1‑101 (MQ=60)
                                                     AGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGNNN                                          >  SRR4302518.10510673/1‑101 (MQ=60)
                                                       NNNCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA                                        <  SRR4302518.10133341/101‑1 (MQ=60)
                                                        NNNCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGacaaacg                                       <  SRR4302518.12849492/101‑8 (MQ=60)
                                                         CCCGGTGGATAAAATCCGCAGGCGGCCCCGTCGGGTTACCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACC                                      <  SRR4302518.5671033/101‑1 (MQ=60)
                                                          NCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCNNNANNCCACAGncnnnnnnnnnncn                                     >  SRR4302518.3551912/1‑87 (MQ=54)
                                                             nnntataaagtccgcAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT                                  <  SRR4302518.13550992/86‑1 (MQ=60)
                                                             GCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT                                  >  SRR4302518.696155/1‑101 (MQ=60)
                                                             GCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGNNN                                  >  SRR4302518.7451656/1‑101 (MQ=60)
                                                             GCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGNNN                                  >  SRR4302518.13732738/1‑101 (MQ=60)
                                                              CGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTG                                 >  SRR4302518.1980738/1‑101 (MQ=60)
                                                              CGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTNNN                                 >  SRR4302518.8089175/1‑101 (MQ=60)
                                                              CGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTNNN                                 >  SRR4302518.10262713/1‑101 (MQ=60)
                                                               nnntaaaaaccgcAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTTC                                <  SRR4302518.12365488/88‑1 (MQ=60)
                                                               gcataaaacccgcAGGCGGCGCCGTCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCACCACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGC                                <  SRR4302518.6924834/88‑1 (MQ=60)
                                                               GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGC                                <  SRR4302518.2761459/101‑1 (MQ=60)
                                                               GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGC                                >  SRR4302518.4917032/1‑101 (MQ=60)
                                                                 nnnaaatccgcaggcgtgGCCGACTGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAC                              <  SRR4302518.9289790/83‑1 (MQ=60)
                                                                 NNNAAATCCGCAGGCGGCGCCGACGGTTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCCG                              <  SRR4302518.11641314/101‑1 (MQ=60)
                                                                 NNNAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAG                              <  SRR4302518.13183167/101‑1 (MQ=60)
                                                                 ATAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAG                              <  SRR4302518.5087765/101‑1 (MQ=60)
                                                                  TAAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGC                             >  SRR4302518.69529/1‑101 (MQ=60)
                                                                   AAAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCTGCAAACCACAGCGCGGGCAGCACCGCTTGCAGCA                            >  SRR4302518.5013476/1‑101 (MQ=60)
                                                                    nnntccgcAGGCGGCGCCGACGGGTTATGG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAC                           <  SRR4302518.13136060/93‑1 (MQ=60)
                                                                    aaacccgccGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑ACTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAC                           <  SRR4302518.2766838/92‑1 (MQ=60)
                                                                    AAATCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGNNN                           >  SRR4302518.9260495/1‑101 (MQ=60)
                                                                       nnngcAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAG                        <  SRR4302518.8209120/96‑1 (MQ=60)
                                                                       nnngcAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACAGTTTGCAGCACCAG                        <  SRR4302518.13701883/96‑1 (MQ=60)
                                                                       TCCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAG                        <  SRR4302518.3542368/101‑1 (MQ=60)
                                                                        nnncAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGCCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGC                       <  SRR4302518.14135916/97‑1 (MQ=60)
                                                                        ccccAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCAGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGC                       <  SRR4302518.1635770/97‑1 (MQ=60)
                                                                        CCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCNNN                       >  SRR4302518.12474639/1‑101 (MQ=60)
                                                                        CCGCAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGC                       <  SRR4302518.2050757/101‑1 (MQ=60)
                                                                         cgcAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGTCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCG                      <  SRR4302518.6211383/98‑1 (MQ=60)
                                                                         cgcAGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGGCCATCGGCAAACCACAGTGCGGGCAGCACCGTTTGCAGCACCANNN                      >  SRR4302518.13166953/4‑101 (MQ=60)
                                                                          N‑NNGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTA                    <  SRR4302518.12512825/101‑1 (MQ=60)
                                                                            AGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTAC                   >  SRR4302518.3166856/1‑101 (MQ=60)
                                                                            AGGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGCCCATCGCCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGNNN                   >  SRR4302518.13089946/1‑101 (MQ=60)
                                                                             GTCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAGCACGCGAAGGTCCATCGGCAAACCACAGCGCGTGCAGCACCGTTTGCAGCACCAGCGTNNN                  >  SRR4302518.10626393/1‑101 (MQ=60)
                                                                             GGCGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACA                  <  SRR4302518.5872964/101‑1 (MQ=60)
                                                                               CGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACNNN                >  SRR4302518.7509514/1‑101 (MQ=60)
                                                                               CGGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAAC                >  SRR4302518.3865253/1‑101 (MQ=60)
                                                                                NNNGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACT               <  SRR4302518.9816770/101‑1 (MQ=60)
                                                                                GGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCCCTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCCCCGTTTGCAGCACCAGCGTACAACT               >  SRR4302518.6492853/1‑101 (MQ=60)
                                                                                GGCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACT               <  SRR4302518.7057152/101‑1 (MQ=60)
                                                                                 GCGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTT              <  SRR4302518.1702878/101‑1 (MQ=60)
                                                                                  CGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTC             <  SRR4302518.5272192/101‑1 (MQ=60)
                                                                                  CGCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTC             <  SRR4302518.4394832/101‑1 (MQ=60)
                                                                                   nnnggCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCC            <  SRR4302518.10088638/96‑1 (MQ=60)
                                                                                   gccccCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACACCTTCC            <  SRR4302518.347331/96‑1 (MQ=60)
                                                                                   NNNGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCC            <  SRR4302518.14233540/101‑1 (MQ=60)
                                                                                   NNNGACGGGTAATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACCCGCGAGGGTCCATCGGCAAACCACAGCGCGGGCAGCCCCGTTTGCAGCACCAGCGTACAACTTCC            <  SRR4302518.10741949/101‑1 (MQ=60)
                                                                                   GCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACGACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCC            >  SRR4302518.3087890/1‑101 (MQ=60)
                                                                                   GCCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTNNN            >  SRR4302518.12073016/1‑101 (MQ=60)
                                                                                   GCCGACGGGGTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGTCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCC            >  SRR4302518.1652666/1‑101 (MQ=60)
                                                                                    CGGACGGGTTATCG‑‑GT‑‑‑GCCG‑CAGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAGCTTNNN           >  SRR4302518.13458631/1‑101 (MQ=60)
                                                                                    CCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTNNN           >  SRR4302518.7811258/1‑101 (MQ=60)
                                                                                    CCGACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGGCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTNNN           >  SRR4302518.12149376/1‑101 (MQ=60)
                                                                                     NNNCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGG          <  SRR4302518.12115776/101‑1 (MQ=60)
                                                                                     NNNCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGG          <  SRR4302518.8971019/101‑1 (MQ=60)
                                                                                     CGACGGGTTCTCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGG          <  SRR4302518.5031982/101‑1 (MQ=60)
                                                                                      GACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCNNN         >  SRR4302518.12971038/1‑101 (MQ=60)
                                                                                      GACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGACCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC         >  SRR4302518.5005863/1‑101 (MQ=60)
                                                                                       CCGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCG        <  SRR4302518.2239204/101‑1 (MQ=60)
                                                                                       ACGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCG        >  SRR4302518.5415922/1‑101 (MQ=60)
                                                                                        CGGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGAAGCACCGgtggcagcagcagagtacacctcgcggnnn       >  SRR4302518.13505321/1‑71 (MQ=60)
                                                                                         GGGTTATCT‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAAATTCCAGCGCT      >  SRR4302518.400670/1‑101 (MQ=60)
                                                                                         GGGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTACATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCNNN      >  SRR4302518.10645052/1‑101 (MQ=60)
                                                                                          GGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGNNN     >  SRR4302518.7521207/1‑101 (MQ=60)
                                                                                          GGTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTA     <  SRR4302518.2280022/101‑1 (MQ=60)
                                                                                           GTTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGTCGCNNN    >  SRR4302518.13515670/1‑101 (MQ=60)
                                                                                            TTATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACC   <  SRR4302518.5183739/101‑1 (MQ=60)
                                                                                             TATCG‑‑GT‑‑‑GCCGCC‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCG  <  SRR4302518.6868118/101‑1 (MQ=60)
                                                                                                        |                                                                                               
GGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCG‑‑GT‑‑CGCCG‑C‑GCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCG  >  NC_000913/3554014‑3554206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑