Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,893	+C	coding (405/423 nt)	yhaQ ←	putative L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,891	1	.	C	99.2%	880.6 / ‑1.3	255	coding (407/423 nt)	yhaQ	putative L‑serine dehydratase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (125/128); total (126/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 277 total aligned reads displayed.

GTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256799‑3256978

gTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGt                                                                                          >  1:1287046/1‑93 (MQ=255)

  ttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cc                                                                                       >  1:2337/1‑93 (MQ=255)

   tCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccc                                                                                      <  1:5924320/93‑2 (MQ=255)

   tCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccc                                                                                      >  1:1850083/1‑92 (MQ=255)

     gACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccctt                                                                                    >  2:520084/1‑90 (MQ=255)

      aCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cccttc                                                                                   >  1:2819463/1‑89 (MQ=255)

         tCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAg                                                                                 >  2:3989470/1‑93 (MQ=255)

         tCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAg                                                                                 <  2:5654198/93‑1 (MQ=255)

           gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGc                                                                               >  1:6004765/1‑93 (MQ=255)

             gCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAg                                                                             >  1:3852638/1‑93 (MQ=255)

             gCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAg                                                                             <  1:4227090/93‑1 (MQ=255)

             gCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCAACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAg                                                                             <  1:3699587/93‑1 (MQ=255)

              cGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGt                                                                            >  2:1551831/1‑93 (MQ=255)

               gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTg                                                                           >  2:1368261/1‑93 (MQ=255)

               gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTg                                                                           <  2:4900792/93‑1 (MQ=255)

               gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTg                                                                           >  2:1806913/1‑93 (MQ=255)

                aCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGa                                                                          <  2:3600881/93‑1 (MQ=255)

                aCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGa                                                                          <  2:4667610/93‑1 (MQ=255)

                 cAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAt                                                                         >  2:2569986/1‑93 (MQ=255)

                  aGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATc                                                                        >  2:4790790/1‑93 (MQ=255)

                  aGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATc                                                                        <  2:2197976/93‑1 (MQ=255)

                   ggCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATcc                                                                       >  1:3601626/1‑93 (MQ=255)

                        aaGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAtt                                                                  >  2:3175774/1‑93 (MQ=255)

                         aGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTc                                                                 >  1:1652891/1‑93 (MQ=255)

                          gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTcc                                                                >  2:5820213/1‑93 (MQ=255)

                            gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGt                                                              >  1:6264756/1‑93 (MQ=255)

                            gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGt                                                              <  1:961837/93‑1 (MQ=255)

                             ttcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGtt                                                             >  2:4730644/1‑93 (MQ=255)

                             ttcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGtt                                                             <  1:762827/93‑1 (MQ=255)

                              tcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtc                                                            >  2:5532404/1‑93 (MQ=255)

                                ttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtctc                                                          >  1:1092166/1‑93 (MQ=255)

                                ttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtctc                                                          >  2:4835138/1‑93 (MQ=255)

                                 tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         <  2:1199907/93‑1 (MQ=255)

                                    tcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATgg                                                      <  2:5043459/93‑1 (MQ=255)

                                     cGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGc                                                     <  1:4591504/93‑1 (MQ=255)

                                     cGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGc                                                     <  1:4398842/93‑1 (MQ=255)

                                         aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 <  2:1157794/93‑1 (MQ=255)

                                          aTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGc                                                >  2:6012728/1‑93 (MQ=255)

                                          aTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCa                                               >  1:614900/1‑93 (MQ=255)

                                            aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa                                              <  2:4928720/93‑1 (MQ=255)

                                             aaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAc                                             <  2:1883463/93‑1 (MQ=255)

                                              aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa                                            <  2:3865643/93‑1 (MQ=255)

                                              aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa                                            >  2:2344781/1‑93 (MQ=255)

                                              aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa                                            >  1:315188/1‑93 (MQ=255)

                                               cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAt                                           <  2:717231/93‑1 (MQ=255)

                                                ccgccgccgACAGAGTGATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                          >  2:2867707/1‑93 (MQ=255)

                                                ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                          <  2:3021177/93‑1 (MQ=255)

                                                 cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                         >  1:2954420/1‑93 (MQ=255)

                                                  gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc                                        <  1:733293/93‑1 (MQ=255)

                                                   ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                       >  2:3775938/1‑93 (MQ=255)

                                                   ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                       <  2:6151389/93‑1 (MQ=255)

                                                   ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                       <  1:2244779/93‑1 (MQ=255)

                                                    cgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg                                      >  1:255570/1‑93 (MQ=255)

                                                    cgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg                                      <  2:3453603/93‑1 (MQ=255)

                                                    cgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg                                      <  2:3832538/93‑1 (MQ=255)

                                                      ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGa                                    <  2:4319681/93‑1 (MQ=255)

                                                      ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGa                                    <  1:3380991/93‑1 (MQ=255)

                                                      ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGa                                    >  1:6384992/1‑93 (MQ=255)

                                                      ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGa                                    <  1:1263137/93‑1 (MQ=255)

                                                       cgACAGAGTAATAGGTTTTACTTAATAGCTCTGCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAt                                   <  2:5336341/93‑1 (MQ=255)

                                                         aCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg                                 <  1:1154483/93‑1 (MQ=255)

                                                         aCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg                                 <  1:5285213/93‑1 (MQ=255)

                                                           agagTAATGGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCTGGATGGaa                               >  2:4402635/1‑93 (MQ=255)

                                                           agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa                               >  1:4040573/1‑93 (MQ=255)

                                                            gagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg                              >  1:2124437/1‑93 (MQ=255)

                                                             agTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGa                             <  2:2798642/93‑1 (MQ=255)

                                                                aaTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAtga                          <  2:6376386/93‑3 (MQ=255)

                                                                 aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAt                         >  1:572797/1‑93 (MQ=255)

                                                                  tAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                        >  2:3211997/1‑93 (MQ=255)

                                                                  tAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                        >  2:577152/1‑93 (MQ=255)

                                                                  tAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                        <  1:6338683/93‑1 (MQ=255)

                                                                   aGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                       <  1:3045346/93‑1 (MQ=255)

                                                                   aGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                       >  1:5686710/1‑93 (MQ=255)

                                                                    ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt                      >  2:4136834/1‑93 (MQ=255)

                                                                    ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt                      >  1:3538005/1‑93 (MQ=255)

                                                                    ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt                      <  1:1500459/93‑1 (MQ=255)

                                                                    ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt                      <  2:3302626/93‑1 (MQ=255)

                                                                     gTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc                     >  2:5932953/1‑93 (MQ=255)

                                                                       tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt                   <  1:5157705/93‑1 (MQ=255)

                                                                       tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt                   <  1:2645323/93‑1 (MQ=255)

                                                                       tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt                   >  1:4500932/1‑93 (MQ=255)

                                                                       tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt                   <  2:2541957/93‑1 (MQ=255)

                                                                       tttACTAAATGGCTCTTCCTGTCCCTTCCAGGCAGTTATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt                   <  1:4210810/93‑1 (MQ=255)

                                                                        ttccTTAATAGCTCTGCCTGTACCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCttt                  <  1:3202440/90‑1 (MQ=255)

                                                                         tACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTg                 >  2:2761047/1‑93 (MQ=255)

                                                                          aCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                >  2:5090057/1‑93 (MQ=255)

                                                                          aCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                <  1:4862924/93‑1 (MQ=255)

                                                                            ttAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTa              >  2:2439232/1‑93 (MQ=255)

                                                                             tAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             >  2:5597464/1‑93 (MQ=255)

                                                                             tAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             >  2:97131/1‑93 (MQ=255)

                                                                              aaTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACa            <  1:1877865/93‑1 (MQ=255)

                                                                              aaTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACa            >  1:136340/1‑93 (MQ=255)

                                                                                tAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAgg          <  1:653220/93‑1 (MQ=255)

                                                                                 aGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGa         <  2:5421488/93‑1 (MQ=255)

                                                                                  gCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaa        >  2:1629848/1‑93 (MQ=255)

                                                                                   ctctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaa       >  2:1316951/1‑93 (MQ=255)

                                                                                   ctctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaa       >  1:1391576/1‑93 (MQ=255)

                                                                                    tctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa      >  2:5074742/1‑93 (MQ=255)

                                                                                     ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt     >  2:5753849/1‑93 (MQ=255)

                                                                                      ttCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc    <  2:2039261/93‑1 (MQ=255)

GTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256799‑3256978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  NC_000913/3256800‑3256986

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC                                                                                               >  SRR4302537.2352/1‑94 (MQ=60)

 TTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCC                                                                                              >  SRR4302537.1862952/1‑94 (MQ=60)

  TCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCCT                                                                                             <  SRR4302537.5964975/94‑1 (MQ=60)

  TCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCCT                                                                                             >  SRR4302537.3334950/1‑94 (MQ=60)

   CGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCctt                                                                                            >  SRR4302537.5864288/1‑91 (MQ=60)

    GACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTC                                                                                            <  SRR4302537.2705351/94‑1 (MQ=60)

    GACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTC                                                                                            >  SRR4302537.2839177/1‑94 (MQ=60)

     ACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCC                                                                                           >  SRR4302537.4802815/1‑94 (MQ=60)

      CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCA                                                                                          <  SRR4302537.1432399/94‑1 (MQ=60)

      CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCA                                                                                          >  SRR4302537.5927484/1‑94 (MQ=60)

      CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCA                                                                                          >  SRR4302537.1577835/1‑94 (MQ=60)

       ATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAG                                                                                         <  SRR4302537.3944561/94‑1 (MQ=60)

         CGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGC                                                                                       >  SRR4302537.6046016/1‑94 (MQ=60)

         CGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGC                                                                                       >  SRR4302537.257998/1‑94 (MQ=60)

           TGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAG                                                                                     >  SRR4302537.3879612/1‑94 (MQ=60)

            GCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGT                                                                                    <  SRR4302537.4256525/94‑1 (MQ=60)

            GCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCAACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGT                                                                                    <  SRR4302537.3725615/94‑1 (MQ=60)

               ACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAT                                                                                 >  SRR4302537.360198/1‑94 (MQ=60)

               ACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAT                                                                                 <  SRR4302537.4547903/94‑1 (MQ=60)

                CAGGCCGAAGTTGTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATC                                                                                <  SRR4302537.2970895/94‑1 (MQ=60)

                CAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATC                                                                                >  SRR4302537.2349489/1‑94 (MQ=60)

                 AGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCC                                                                               >  SRR4302537.3627011/1‑94 (MQ=60)

                   GCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTAATCCGC                                                                             >  SRR4302537.2082662/1‑94 (MQ=60)

                    CCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCA                                                                            >  SRR4302537.1187369/1‑94 (MQ=60)

                    CCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCA                                                                            >  SRR4302537.628729/1‑94 (MQ=60)

                      GAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATT                                                                          >  SRR4302537.6435221/1‑94 (MQ=60)

                       AAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTC                                                                         >  SRR4302537.1664479/1‑94 (MQ=60)

                       AAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTC                                                                         >  SRR4302537.1506079/1‑94 (MQ=60)

                        atttttttttTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCACATTCC                                                                        <  SRR4302537.4032988/84‑1 (MQ=60)

                          TGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGT                                                                      >  SRR4302537.6307758/1‑94 (MQ=60)

                           GTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTT                                                                     >  SRR4302537.5257432/1‑94 (MQ=60)

                           GTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTT                                                                     <  SRR4302537.968476/94‑1 (MQ=60)

                            TTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTC                                                                    <  SRR4302537.768042/94‑1 (MQ=60)

                             TCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCT                                                                   <  SRR4302537.137193/94‑1 (MQ=60)

                              CTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTC                                                                  >  SRR4302537.1099708/1‑94 (MQ=60)

                              CTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTC                                                                  <  SRR4302537.5634680/94‑1 (MQ=60)

                                TCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                                <  SRR4302537.1279619/94‑1 (MQ=60)

                                TCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                                <  SRR4302537.3028470/94‑1 (MQ=60)

                                 CTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATG                                                               <  SRR4302537.1783484/94‑1 (MQ=60)

                                 CTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATG                                                               >  SRR4302537.2916503/1‑94 (MQ=60)

                                  TTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGG                                                              <  SRR4302537.2508573/94‑1 (MQ=60)

                                  TTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGG                                                              <  SRR4302537.5155470/94‑1 (MQ=60)

                                   TCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGC                                                             <  SRR4302537.6185014/94‑1 (MQ=60)

                                    CGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCG                                                            >  SRR4302537.1157847/1‑94 (MQ=60)

                                    CGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCG                                                            <  SRR4302537.4429195/94‑1 (MQ=60)

                                    CGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCG                                                            <  SRR4302537.4623266/94‑1 (MQ=60)

                                    CGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCG                                                            >  SRR4302537.4419512/1‑94 (MQ=60)

                                       CAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                         <  SRR4302537.2452446/94‑1 (MQ=60)

                                        AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                        >  SRR4302537.2170777/1‑94 (MQ=60)

                                        AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                        <  SRR4302537.4107005/94‑1 (MQ=60)

                                        AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCA                                                       >  SRR4302537.619090/1‑94 (MQ=60)

                                          AAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAAGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAA                                                      <  SRR4302537.4530330/94‑1 (MQ=60)

                                            AACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACA                                                    >  SRR4302537.317376/1‑94 (MQ=60)

                                              CCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATT                                                  >  SRR4302537.6427545/1‑94 (MQ=60)

                                               CCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTT                                                 >  SRR4302537.2975153/1‑94 (MQ=60)

                                               CCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTT                                                 >  SRR4302537.5992363/1‑94 (MQ=60)

                                                CGCCGCCGACAGAGTAATAGGTTTTACGTAATAGCTCTTCCTGTCCCTTCCAGGCAGGGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTC                                                <  SRR4302537.1174421/94‑1 (MQ=60)

                                                 GCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCG                                               <  SRR4302537.738297/94‑1 (MQ=60)

                                                  CCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGG                                              <  SRR4302537.6957/94‑1 (MQ=60)

                                                  CCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGG                                              >  SRR4302537.257381/1‑94 (MQ=60)

                                                  CCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGG                                              <  SRR4302537.1875938/94‑1 (MQ=60)

                                                  CCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGG                                              <  SRR4302537.2260614/94‑1 (MQ=60)

                                                   CGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGG                                             >  SRR4302537.6311843/1‑94 (MQ=60)

                                                    GCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGA                                            >  SRR4302537.6428509/1‑94 (MQ=60)

                                                     CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           <  SRR4302537.752684/94‑1 (MQ=60)

                                                     CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           <  SRR4302537.388738/94‑1 (MQ=60)

                                                     CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           <  SRR4302537.1271922/94‑1 (MQ=60)

                                                     CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           >  SRR4302537.3432507/1‑94 (MQ=60)

                                                     CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           <  SRR4302537.3404945/94‑1 (MQ=60)

                                                      CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          <  SRR4302537.4597702/94‑1 (MQ=60)

                                                      CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          <  SRR4302537.3100594/94‑1 (MQ=60)

                                                       GACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGG                                         >  SRR4302537.641075/1‑94 (MQ=60)

                                                       GACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGG                                         <  SRR4302537.5967328/94‑1 (MQ=60)

                                                        ACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGA                                        <  SRR4302537.1162518/94‑1 (MQ=60)

                                                        ACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGA                                        >  SRR4302537.3663076/1‑94 (MQ=60)

                                                        ACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGA                                        <  SRR4302537.5321557/94‑1 (MQ=60)

                                                         CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                       >  SRR4302537.1502347/1‑94 (MQ=60)

                                                         CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                       >  SRR4302537.4068838/1‑94 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      >  SRR4302537.2139381/1‑94 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      >  SRR4302537.5102177/1‑94 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      <  SRR4302537.227872/94‑1 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      <  SRR4302537.5747982/94‑1 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      <  SRR4302537.616822/94‑1 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      >  SRR4302537.1292827/1‑94 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATAGAAG                                      >  SRR4302537.3236056/1‑94 (MQ=60)

                                                             GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   <  SRR4302537.521813/94‑1 (MQ=60)

                                                              TAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAA                                  <  SRR4302537.3193244/94‑1 (MQ=60)

                                                               AATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302537.576728/1‑94 (MQ=60)

                                                               AATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302537.4467415/1‑94 (MQ=60)

                                                               AATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302537.6269589/1‑94 (MQ=60)

                                                               AATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302537.4765359/1‑94 (MQ=60)

                                                                 TAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                               >  SRR4302537.5725607/1‑94 (MQ=60)

                                                                 TAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                               <  SRR4302537.6382001/94‑1 (MQ=60)

                                                                  AGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              >  SRR4302537.3562994/1‑94 (MQ=60)

                                                                  AGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              <  SRR4302537.5607938/94‑1 (MQ=60)

                                                                  AGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              <  SRR4302537.3066559/94‑1 (MQ=60)

                                                                   GGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTC                             <  SRR4302537.1510934/94‑1 (MQ=60)

                                                                   GGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTC                             >  SRR4302537.1646783/1‑94 (MQ=60)

                                                                   GGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTC                             <  SRR4302537.4144026/94‑1 (MQ=60)

                                                                    GTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCT                            >  SRR4302537.3062641/1‑94 (MQ=60)

                                                                     TTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT                           <  SRR4302537.384127/94‑1 (MQ=60)

                                                                     TTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT                           >  SRR4302537.4532059/1‑94 (MQ=60)

                                                                      TTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                          >  SRR4302537.4415167/1‑94 (MQ=60)

                                                                      TTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                          <  SRR4302537.2663825/94‑1 (MQ=60)

                                                                      TTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                          <  SRR4302537.5193247/94‑1 (MQ=60)

                                                                      TTTACTAAATGGCTCTTCCTGTCCCTTCCAGGCAGTTATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                          <  SRR4302537.4240180/94‑1 (MQ=60)

                                                                       TTCCTTAATAGCTCTGCCTGTACCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTG                         <  SRR4302537.3224954/94‑1 (MQ=60)

                                                                       TTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTG                         <  SRR4302537.697382/94‑1 (MQ=60)

                                                                        TACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGC                        >  SRR4302537.1318838/1‑94 (MQ=60)

                                                                         ACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCT                       <  SRR4302537.4896474/94‑1 (MQ=60)

                                                                         ACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCT                       <  SRR4302537.2082110/94‑1 (MQ=60)

                                                                           TTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                     <  SRR4302537.6465915/94‑1 (MQ=60)

                                                                           TTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                     >  SRR4302537.802005/1‑94 (MQ=60)

                                                                            TAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACA                    >  SRR4302537.137315/1‑94 (MQ=60)

                                                                             AATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                   <  SRR4302537.1890927/94‑1 (MQ=60)

                                                                             AATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                   <  SRR4302537.2715081/94‑1 (MQ=60)

                                                                              ATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGG                  <  SRR4302537.4255897/94‑1 (MQ=60)

                                                                               TAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGA                 <  SRR4302537.657673/94‑1 (MQ=60)

                                                                                AGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAA                >  SRR4302537.1399952/1‑94 (MQ=60)

                                                                                AGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAA                >  SRR4302537.1722079/1‑94 (MQ=60)

                                                                                 GCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAA               >  SRR4302537.1401279/1‑94 (MQ=60)

                                                                                   TCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAT             >  SRR4302537.3766470/1‑94 (MQ=60)

                                                                                     TTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCA           <  SRR4302537.815129/94‑1 (MQ=60)

                                                                                       CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302537.1054821/1‑94 (MQ=60)

                                                                                       CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302537.70343/1‑94 (MQ=60)

                                                                                        CTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302537.4085071/94‑1 (MQ=60)

                                                                                        CTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        >  SRR4302537.881383/1‑94 (MQ=60)

                                                                                        CTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302537.2147351/94‑1 (MQ=60)

                                                                                        CTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302537.5470390/94‑1 (MQ=60)

                                                                                        CTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302537.712669/94‑1 (MQ=60)

                                                                                        CTGTCCCTTCCAGGCAGTGATCCGAATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302537.3599968/94‑1 (MQ=60)

                                                                                         TGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA       >  SRR4302537.1039544/1‑94 (MQ=60)

                                                                                         TGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA       >  SRR4302537.1210091/1‑94 (MQ=60)

                                                                                          gtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      <  SRR4302537.486357/91‑1 (MQ=60)

                                                                                          gtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      <  SRR4302537.6153371/91‑1 (MQ=60)

                                                                                          gtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      <  SRR4302537.4017407/91‑1 (MQ=60)

                                                                                           tcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATA     <  SRR4302537.3371998/92‑1 (MQ=60)

                                                                                           tcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATA     >  SRR4302537.4614914/3‑94 (MQ=60)

                                                                                           tcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATA     >  SRR4302537.3474962/3‑94 (MQ=60)

                                                                                           C‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATAT    <  SRR4302537.5194481/94‑1 (MQ=60)

                                                                                           C‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATAT    >  SRR4302537.705263/1‑94 (MQ=60)

                                                                                           C‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATAT    >  SRR4302537.2148653/1‑94 (MQ=60)

                                                                                           C‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATAT    >  SRR4302537.3978371/1‑94 (MQ=60)

                                                                                             CCTTCCAGGCAGTGATCCGTATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG   <  SRR4302537.2670751/94‑1 (MQ=60)

                                                                                             CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGCAAATCAACAATATG   >  SRR4302537.3301328/1‑94 (MQ=60)

                                                                                             CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG   >  SRR4302537.5469031/1‑94 (MQ=60)

                                                                                              CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  SRR4302537.4956875/1‑94 (MQ=60)

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  NC_000913/3256800‑3256986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`