Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,349,294 T→C Q456R (CAG→CGG)  arcB ← aerobic respiration sensor‑response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,349,2940TC100.0% 872.1 / NA 250Q456R (CAG→CGG) arcBaerobic respiration sensor‑response protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (120/130);  total (120/130)

Only 100 of 252 total aligned reads displayed.

AATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCA  >  NC_000913/3349206‑3349383
                                                                                        |                                                                                         
gccgcccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTa                                                                                       <  2:4221150‑M1/41‑1 (MQ=255)
 ccgcccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTaa                                                                                      >  1:5856259‑M1/52‑93 (MQ=255)
 ccgcccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTaa                                                                                      >  1:2408651‑M1/52‑93 (MQ=255)
  cgcccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAAt                                                                                     >  2:958104‑M1/51‑93 (MQ=255)
  cgcccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAAt                                                                                     >  1:138658‑M1/51‑93 (MQ=255)
    cccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATAc                                                                                   >  1:1136139‑M1/49‑93 (MQ=255)
    cccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATAc                                                                                   <  2:3170801‑M1/45‑1 (MQ=255)
     ccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACa                                                                                  <  1:5026062‑M1/46‑1 (MQ=255)
     ccatatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACa                                                                                  >  1:4936618‑M1/48‑93 (MQ=255)
      catatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAt                                                                                 >  1:1068749‑M1/47‑93 (MQ=255)
      catatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAt                                                                                 <  2:4254845‑M1/47‑1 (MQ=255)
      catatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAt                                                                                 >  1:6324333‑M1/47‑93 (MQ=255)
        tatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATgg                                                                               <  1:2236555‑M1/49‑1 (MQ=255)
        tatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATgg                                                                               >  1:3831778‑M1/45‑93 (MQ=255)
        tatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATgg                                                                               >  1:6257692‑M1/45‑93 (MQ=255)
        tatttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGGTTACCGCCATGACTGTCTTTCACCCGGTAATACATgg                                                                               <  1:154399‑M1/49‑1 (MQ=255)
         atttttcgccagacgacgagaagcggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGc                                                                              >  2:5936924‑M1/44‑93 (MQ=255)
           ttttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGa                                                                            <  1:4395069‑M1/52‑1 (MQ=255)
            tttcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGaa                                                                           >  1:5342999‑M1/41‑93 (MQ=255)
              tcgccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGaaaa                                                                         <  2:429024‑M1/55‑1 (MQ=255)
               cgccagacgacgacaaacgcccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAt                                                                        >  1:2553969‑M1/38‑93 (MQ=255)
                gccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtt                                                                       <  2:6088881‑M1/57‑1 (MQ=255)
                gccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtt                                                                       >  1:4915579‑M1/37‑93 (MQ=255)
                gccagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtt                                                                       <  2:3062531‑M1/57‑1 (MQ=255)
                  cagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtttt                                                                     <  2:6108173‑M1/59‑1 (MQ=255)
                  cagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtttt                                                                     >  2:4796709‑M1/35‑93 (MQ=255)
                  cagacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtttt                                                                     <  2:1570372‑M1/59‑1 (MQ=255)
                  cagacgacgagaaacggcaagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtttt                                                                     >  2:227179‑M1/35‑93 (MQ=255)
                   agacgactagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTa                                                                    <  1:532464‑M1/60‑1 (MQ=255)
                    gacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTAt                                                                   >  1:2080131‑M1/33‑93 (MQ=255)
                    gacgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTAt                                                                   <  2:3409191‑M1/61‑1 (MQ=255)
                      cgacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATcc                                                                 >  2:2972814‑M1/31‑93 (MQ=255)
                       gacgagaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCa                                                                <  2:2111320‑M1/64‑1 (MQ=255)
                           agaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTc                                                            >  1:4689428‑M1/26‑93 (MQ=255)
                           agaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTc                                                            <  2:4066484‑M1/68‑1 (MQ=255)
                           agaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTc                                                            <  2:4712127‑M1/68‑1 (MQ=255)
                            gaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCa                                                           <  1:1977327‑M1/69‑1 (MQ=255)
                            gaaacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCa                                                           >  1:6000955‑M1/25‑93 (MQ=255)
                              aacggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATc                                                         <  2:3760167‑M1/71‑1 (MQ=255)
                               acggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATcc                                                        >  1:4087645‑M1/22‑93 (MQ=255)
                               acggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATcc                                                        >  2:5399812‑M1/22‑93 (MQ=255)
                               acggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATcc                                                        >  2:6412806‑M1/22‑93 (MQ=255)
                                cggccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCt                                                       <  1:1005036‑M1/73‑1 (MQ=255)
                                  gccagaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGc                                                     >  1:3989458‑M1/19‑93 (MQ=255)
                                     agaccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGa                                                  <  1:150929‑M1/78‑1 (MQ=255)
                                        ccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg                                               >  1:4470446‑M1/13‑93 (MQ=255)
                                         caataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGc                                              <  1:5110289‑M1/82‑1 (MQ=255)
                                         caataccggtcCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGc                                              >  1:5677326‑M1/12‑93 (MQ=255)
                                          aataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGcc                                             <  1:3021880‑M1/83‑1 (MQ=255)
                                          aataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGcc                                             >  1:1900172‑M1/11‑93 (MQ=255)
                                          aataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGcc                                             >  2:3953017‑M1/11‑93 (MQ=255)
                                           ataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaatggcg                                            <  2:1122934‑M1/84‑4 (MQ=255)
                                            taccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGa                                           >  2:2020648‑M1/9‑93 (MQ=255)
                                               cggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATAc                                        >  1:3627355‑M1/6‑93 (MQ=255)
                                                ggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATAcc                                       >  1:2732314‑M1/5‑93 (MQ=255)
                                                 gtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCa                                      >  2:3463812‑M1/4‑93 (MQ=255)
                                                 gtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGACGATACCa                                      <  2:780566‑M1/90‑1 (MQ=255)
                                                   tccGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCaga                                    <  1:5705293/92‑1 (MQ=255)
                                                   gCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCaga                                    >  2:5158739‑M1/2‑93 (MQ=255)
                                                     cGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGt                                  <  1:3311097/93‑1 (MQ=255)
                                                     cGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGt                                  <  2:2813096/93‑1 (MQ=255)
                                                       gTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCt                                <  2:5790873/93‑1 (MQ=255)
                                                       gTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCt                                >  1:5934881/1‑93 (MQ=255)
                                                       gTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCt                                <  2:3659011/93‑1 (MQ=255)
                                                         ggCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTc                              <  1:4817488/93‑1 (MQ=255)
                                                         ggCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTc                              >  1:4864163/1‑93 (MQ=255)
                                                            aGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCAc                           >  2:2620870/1‑93 (MQ=255)
                                                            aGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCAc                           <  1:5267725/93‑1 (MQ=255)
                                                             ggTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAAtttt                                                                     >  1:1310131/1‑50 (MQ=255)
                                                             ggTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACt                          >  2:3372541/1‑93 (MQ=255)
                                                             ggTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACt                          <  1:3347311/93‑1 (MQ=255)
                                                             ggTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACt                          >  2:2504843/1‑93 (MQ=255)
                                                             ggTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACt                          >  2:3267020/1‑93 (MQ=255)
                                                                  aCCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCaaa                     >  2:138536/1‑93 (MQ=255)
                                                                   ccGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCaaaa                    <  2:762621/93‑1 (MQ=255)
                                                                   ccGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCaaaa                    <  2:5255735/93‑1 (MQ=255)
                                                                    cGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAAt                   <  1:4272374/93‑1 (MQ=255)
                                                                    cGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAAt                   <  1:775317/93‑1 (MQ=255)
                                                                       cATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATgca                >  1:968017/1‑93 (MQ=255)
                                                                       cATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATgca                <  2:1321956/93‑1 (MQ=255)
                                                                         tGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATgcagc              <  2:1660702/93‑1 (MQ=255)
                                                                          gACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATgcagca             <  1:2761757/93‑1 (MQ=255)
                                                                          gACTGTCTTTCACCCGGTAATACATGGCGAAAATTATATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATgcagca             <  2:3617033/93‑1 (MQ=255)
                                                                            cTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCata           >  1:1406370/1‑93 (MQ=255)
                                                                             tGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCatat          <  1:6118451/93‑1 (MQ=255)
                                                                              gTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATc         <  1:378333/93‑1 (MQ=255)
                                                                              gTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATc         <  1:3558138/93‑1 (MQ=255)
                                                                              gTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATc         <  1:1763343/93‑1 (MQ=255)
                                                                              gTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATc         <  2:5308250/93‑1 (MQ=255)
                                                                              gTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATc         >  2:5970241/1‑93 (MQ=255)
                                                                              gTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATc         <  2:5993850/93‑1 (MQ=255)
                                                                               tCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCg        <  1:244143/93‑1 (MQ=255)
                                                                               tCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCg        >  1:4574677/1‑93 (MQ=255)
                                                                               tCTTTCACCCGGTAATACACGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCg        >  2:6296552/1‑93 (MQ=255)
                                                                                cTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGc       <  1:6463406/93‑1 (MQ=255)
                                                                                 tttCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGcc      <  2:1071796/93‑1 (MQ=255)
                                                                                 tttCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGcc      >  2:4621113/1‑93 (MQ=255)
                                                                                  ttCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCt     <  1:3256019/93‑1 (MQ=255)
                                                                                   tCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCtt    >  1:4770762/1‑93 (MQ=255)
                                                                                     aCCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCa  <  1:5865713/93‑1 (MQ=255)
                                                                                        |                                                                                         
AATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCA  >  NC_000913/3349206‑3349383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TA‑‑‑‑‑‑CCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGT  >  NC_000913/3349207‑3349387
                                                                                                   |                                                                                             
ATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCC                                                                                               <  SRR4302537.3712344/94‑1 (MQ=60)
ATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCC                                                                                               >  SRR4302537.4085340/1‑94 (MQ=60)
ATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCC                                                                                               >  SRR4302537.1313954/1‑94 (MQ=60)
ATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCC                                                                                               <  SRR4302537.3009159/94‑1 (MQ=60)
     CCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAA                                                                                          <  SRR4302537.1808499/94‑1 (MQ=60)
      CCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAAT                                                                                         <  SRR4302537.4223979/94‑1 (MQ=60)
      CCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAAT                                                                                         <  SRR4302537.2425609/94‑1 (MQ=60)
      CCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAAT                                                                                         <  SRR4302537.5896427/94‑1 (MQ=60)
      CCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAAT                                                                                         <  SRR4302537.537785/94‑1 (MQ=60)
       CGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATA                                                                                        <  SRR4302537.139649/94‑1 (MQ=60)
       CGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATA                                                                                        >  SRR4302537.2512545/1‑94 (MQ=60)
       CGCCCATATGTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATA                                                                                        <  SRR4302537.5742555/94‑1 (MQ=60)
         CCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACA                                                                                      >  SRR4302537.2803643/1‑94 (MQ=60)
         CCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACA                                                                                      >  SRR4302537.5060675/1‑94 (MQ=60)
         CCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACA                                                                                      <  SRR4302537.1144031/94‑1 (MQ=60)
         CCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACA                                                                                      >  SRR4302537.1672342/1‑94 (MQ=60)
          CCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAT                                                                                     <  SRR4302537.2216471/94‑1 (MQ=60)
          CCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAT                                                                                     >  SRR4302537.3943714/1‑94 (MQ=60)
          CCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAT                                                                                     <  SRR4302537.4970664/94‑1 (MQ=60)
          CCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAT                                                                                     <  SRR4302537.5394478/94‑1 (MQ=60)
          CCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAT                                                                                     >  SRR4302537.5077878/1‑94 (MQ=60)
          CCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACAT                                                                                     >  SRR4302537.1345760/1‑94 (MQ=60)
           CATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATG                                                                                    <  SRR4302537.5636636/94‑1 (MQ=60)
           CATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATG                                                                                    <  SRR4302537.1076115/94‑1 (MQ=60)
           CATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATG                                                                                    <  SRR4302537.5865122/94‑1 (MQ=60)
           CATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATG                                                                                    <  SRR4302537.6367597/94‑1 (MQ=60)
            ATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGG                                                                                   <  SRR4302537.4074695/94‑1 (MQ=60)
            ATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGG                                                                                   >  SRR4302537.4625693/1‑94 (MQ=60)
            ATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGG                                                                                   >  SRR4302537.2252328/1‑94 (MQ=60)
            ATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGGTTACCGCCATGACTGTCTTTCACCCGGTAATACATGG                                                                                   >  SRR4302537.155498/1‑94 (MQ=60)
             TATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGC                                                                                  >  SRR4302537.4904591/1‑94 (MQ=60)
             TATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGC                                                                                  <  SRR4302537.3858636/94‑1 (MQ=60)
             TATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGC                                                                                  <  SRR4302537.6300668/94‑1 (MQ=60)
             TATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGC                                                                                  >  SRR4302537.1404691/1‑94 (MQ=60)
               TTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGA                                                                                >  SRR4302537.4425388/1‑94 (MQ=60)
                 TTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAA                                                                              <  SRR4302537.5379614/94‑1 (MQ=60)
                    CGCCAGACGACGAGAAACGGCCAGACCAATACCGGTA‑‑‑‑‑‑CCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATT                                                                           >  SRR4302537.6079457/1‑94 (MQ=60)
                    CGCCAGACGACGACAAACGCCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATT                                                                           <  SRR4302537.2571881/94‑1 (MQ=60)
                     GCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTT                                                                          <  SRR4302537.4949500/94‑1 (MQ=60)
                       CAGACGACTAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTA                                                                        >  SRR4302537.536118/1‑94 (MQ=60)
                         GACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATC                                                                      <  SRR4302537.2094782/94‑1 (MQ=60)
                          ACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCC                                                                     <  SRR4302537.4967734/94‑1 (MQ=60)
                          ACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCC                                                                     <  SRR4302537.4839500/94‑1 (MQ=60)
                           CGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCA                                                                    >  SRR4302537.2636402/1‑94 (MQ=60)
                            GACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTCCCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAG                                                                   >  SRR4302537.4591510/1‑94 (MQ=60)
                              CGAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCT                                                                 >  SRR4302537.3810813/1‑94 (MQ=60)
                               GAGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTC                                                                >  SRR4302537.5007853/1‑94 (MQ=60)
                                AGAAACGTCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCA                                                               <  SRR4302537.829923/94‑1 (MQ=60)
                                AGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCA                                                               >  SRR4302537.1991068/1‑94 (MQ=60)
                                AGAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCA                                                               <  SRR4302537.4721864/94‑1 (MQ=60)
                                 GAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCAT                                                              <  SRR4302537.3002081/94‑1 (MQ=60)
                                 GAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCAT                                                              <  SRR4302537.3307259/94‑1 (MQ=60)
                                 GAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCAT                                                              <  SRR4302537.1284187/94‑1 (MQ=60)
                                 GAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCAT                                                              <  SRR4302537.6042177/94‑1 (MQ=60)
                                 GAAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCAT                                                              >  SRR4302537.5899195/1‑94 (MQ=60)
                                  AAACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGGTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATC                                                             >  SRR4302537.647590/1‑94 (MQ=60)
                                   AACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCC                                                            <  SRR4302537.2706422/94‑1 (MQ=60)
                                    ACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCT                                                           <  SRR4302537.4875110/94‑1 (MQ=60)
                                    ACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCT                                                           <  SRR4302537.4116191/94‑1 (MQ=60)
                                    ACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCT                                                           >  SRR4302537.1011973/1‑94 (MQ=60)
                                    ACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCT                                                           <  SRR4302537.3413328/94‑1 (MQ=60)
                                    ACGGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCT                                                           <  SRR4302537.2039570/94‑1 (MQ=60)
                                      GGCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGC                                                         >  SRR4302537.1989860/1‑94 (MQ=60)
                                       GCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCG                                                        <  SRR4302537.2764327/94‑1 (MQ=60)
                                       GCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCG                                                        <  SRR4302537.4017395/94‑1 (MQ=60)
                                       GCCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATACTGCG                                                        >  SRR4302537.5359342/1‑94 (MQ=60)
                                        CCAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGG                                                       >  SRR4302537.3630538/1‑94 (MQ=60)
                                         CAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGA                                                      >  SRR4302537.4896211/1‑94 (MQ=60)
                                         CAGACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGA                                                      >  SRR4302537.152008/1‑94 (MQ=60)
                                           GACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAAT                                                    <  SRR4302537.5882665/94‑1 (MQ=60)
                                           GACCAA‑‑‑‑‑‑TACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAAT                                                    >  SRR4302537.1339309/1‑94 (MQ=60)
                                                   ccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGC                                                  >  SRR4302537.5145502/13‑94 (MQ=60)
                                                   ccaataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGC                                                  <  SRR4302537.4501364/82‑1 (MQ=60)
                                                    caataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCC                                                 >  SRR4302537.3042944/12‑94 (MQ=60)
                                                    caataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCC                                                 >  SRR4302537.6338223/12‑94 (MQ=60)
                                                    caataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCC                                                 >  SRR4302537.458158/12‑94 (MQ=60)
                                                    caataccggtcCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCC                                                 <  SRR4302537.5716170/83‑1 (MQ=60)
                                                     aataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCG                                                <  SRR4302537.1913369/84‑1 (MQ=60)
                                                      ataccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGA                                               >  SRR4302537.4091664/10‑94 (MQ=60)
                                                       taccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGAT                                              >  SRR4302537.106307/9‑94 (MQ=60)
                                                       taccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGAT                                              <  SRR4302537.229703/86‑1 (MQ=60)
                                                       taccggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGAT                                              <  SRR4302537.3354314/86‑1 (MQ=60)
                                                       TG‑‑‑‑‑‑CCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGA                                        <  SRR4302537.1487384/94‑1 (MQ=60)
                                                        accggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATA                                             >  SRR4302537.5072136/8‑94 (MQ=60)
                                                        T‑‑‑‑‑‑CCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAG                                       <  SRR4302537.5744341/94‑1 (MQ=60)
                                                        G‑‑‑‑‑‑CCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAG                                       <  SRR4302537.4762668/94‑1 (MQ=60)
                                                          cggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACC                                           <  SRR4302537.1131119/89‑1 (MQ=60)
                                                          cggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACC                                           <  SRR4302537.3652921/89‑1 (MQ=60)
                                                           ggtgCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCA                                          <  SRR4302537.2751386/90‑1 (MQ=60)
                                                               CCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGT                                      <  SRR4302537.2446432/94‑1 (MQ=60)
                                                                CGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTC                                     <  SRR4302537.3334521/94‑1 (MQ=60)
                                                                 GGTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCT                                    >  SRR4302537.5975610/1‑94 (MQ=60)
                                                                  GTGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTT                                   >  SRR4302537.3241906/1‑94 (MQ=60)
                                                                   TGGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTC                                  >  SRR4302537.4897724/1‑94 (MQ=60)
                                                                    GGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCC                                 <  SRR4302537.4850753/94‑1 (MQ=60)
                                                                    GGCAGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCC                                 >  SRR4302537.4963033/1‑94 (MQ=60)
                                                                       AGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTAacggaatagatcggaagagcggttcagcaggaatgcggagac                              >  SRR4302537.1319251/1‑52 (MQ=60)
                                                                       AGGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACT                              <  SRR4302537.5303976/94‑1 (MQ=60)
                                                                        GGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTT                             >  SRR4302537.37786/1‑94 (MQ=60)
                                                                        GGTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTT                             <  SRR4302537.3371008/94‑1 (MQ=60)
                                                                         GTTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTC                            <  SRR4302537.5271418/94‑1 (MQ=60)
                                                                          TTTACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCA                           <  SRR4302537.6070961/94‑1 (MQ=60)
                                                                            TACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAA                         <  SRR4302537.2440477/94‑1 (MQ=60)
                                                                             ACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAA                        <  SRR4302537.4477365/94‑1 (MQ=60)
                                                                             ACCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAA                        <  SRR4302537.1082913/94‑1 (MQ=60)
                                                                              CCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAAT                       <  SRR4302537.603730/94‑1 (MQ=60)
                                                                              CCGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAAT                       <  SRR4302537.1516814/94‑1 (MQ=60)
                                                                               CGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATG                      <  SRR4302537.4302060/94‑1 (MQ=60)
                                                                               CGCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATG                      <  SRR4302537.780630/94‑1 (MQ=60)
                                                                                GCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGACTGTCGATACCAGAGTATTCCACTTCAAAATGC                     >  SRR4302537.3462773/1‑94 (MQ=60)
                                                                                GCCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGC                     >  SRR4302537.6242365/1‑94 (MQ=60)
                                                                                 CCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCA                    <  SRR4302537.2152711/94‑1 (MQ=60)
                                                                                 CCATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCA                    >  SRR4302537.974690/1‑94 (MQ=60)
                                                                                  CATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAG                   >  SRR4302537.4391258/1‑94 (MQ=60)
                                                                                   CTGACTGGCTTTCACCCGGTAATACATGGCCAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGC                  <  SRR4302537.592724/94‑1 (MQ=60)
                                                                                   ATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGC                  <  SRR4302537.6490206/94‑1 (MQ=60)
                                                                                   ATGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGC                  <  SRR4302537.5961725/94‑1 (MQ=60)
                                                                                    TGACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCA                 >  SRR4302537.1942435/1‑94 (MQ=60)
                                                                                     GACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCAT                <  SRR4302537.2781025/94‑1 (MQ=60)
                                                                                      ACTGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATA               >  SRR4302537.1416163/1‑94 (MQ=60)
                                                                                        TGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATC             >  SRR4302537.858284/1‑94 (MQ=60)
                                                                                        TGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATC             >  SRR4302537.4801733/1‑94 (MQ=60)
                                                                                        TGTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATC             <  SRR4302537.6160505/94‑1 (MQ=60)
                                                                                         GTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCG            <  SRR4302537.4409918/94‑1 (MQ=60)
                                                                                         GTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCG            >  SRR4302537.4606339/1‑94 (MQ=60)
                                                                                         GTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCG            <  SRR4302537.380955/94‑1 (MQ=60)
                                                                                         GTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCG            <  SRR4302537.3583243/94‑1 (MQ=60)
                                                                                         GTCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCG            <  SRR4302537.1775625/94‑1 (MQ=60)
                                                                                          TCTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGC           <  SRR4302537.245874/94‑1 (MQ=60)
                                                                                           CTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCC          <  SRR4302537.3302038/94‑1 (MQ=60)
                                                                                           CTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCC          >  SRR4302537.721286/1‑94 (MQ=60)
                                                                                           CTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCC          <  SRR4302537.6507192/94‑1 (MQ=60)
                                                                                           CTTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCC          <  SRR4302537.6296568/94‑1 (MQ=60)
                                                                                            TTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCT         <  SRR4302537.367420/94‑1 (MQ=60)
                                                                                            TTTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCT         <  SRR4302537.1552523/94‑1 (MQ=60)
                                                                                             TTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTT        >  SRR4302537.4803701/1‑94 (MQ=60)
                                                                                             TTCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTT        <  SRR4302537.3278980/94‑1 (MQ=60)
                                                                                              TCACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTC       <  SRR4302537.3820873/94‑1 (MQ=60)
                                                                                               CACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCA      >  SRR4302537.1658681/1‑94 (MQ=60)
                                                                                               CACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGGCTTCCACTTCAAAATGCAGCATATCGCCTTCA      >  SRR4302537.5324335/1‑94 (MQ=60)
                                                                                                ACCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCAT     <  SRR4302537.5905954/94‑1 (MQ=60)
                                                                                                 CCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATC    <  SRR4302537.6425427/94‑1 (MQ=60)
                                                                                                 CCCGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATC    <  SRR4302537.6246816/94‑1 (MQ=60)
                                                                                                  CCGGTAACACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCG   >  SRR4302537.4804462/1‑94 (MQ=60)
                                                                                                   CGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGT  >  SRR4302537.6306071/1‑94 (MQ=60)
                                                                                                   CGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGT  >  SRR4302537.679430/1‑94 (MQ=60)
                                                                                                   CGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGT  >  SRR4302537.2046229/1‑94 (MQ=60)
                                                                                                   |                                                                                             
ATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAA‑‑‑‑‑‑TA‑‑‑‑‑‑CCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGT  >  NC_000913/3349207‑3349387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑