Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,417,403 A→G intergenic (+1155/‑368) yhdV → / → b3269 hypothetical protein/putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,417,4030AG97.6% 832.6 / 6.1 249intergenic (+1155/‑368)yhdV/b3269hypothetical protein/putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base G (117/124);  total (119/130)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

Only 100 of 259 total aligned reads displayed.

CCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGCC  >  NC_000913/3417311‑3417492
                                                                                            |                                                                                         
  aGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCg                                                                                         >  1:5621025/1‑93 (MQ=255)
   gCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTgcgc                                                                                        <  1:5881520/93‑1 (MQ=255)
     tGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTg                                                                                      >  1:3069064/1‑93 (MQ=255)
     tGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTg                                                                                      >  1:851023/1‑93 (MQ=255)
      gAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTgg                                                                                     <  2:1879505/93‑1 (MQ=255)
      gAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTgg                                                                                     >  1:4229844/1‑93 (MQ=255)
      gAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTgg                                                                                     >  1:1771197/1‑93 (MQ=255)
       aaTGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTggc                                                                                    >  2:2106395/1‑92 (MQ=255)
       aaTGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGa                                                                                    <  1:2138221/93‑1 (MQ=255)
       aaTGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGa                                                                                    <  1:5518470/93‑1 (MQ=255)
       aaTGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGa                                                                                    >  1:5254162/1‑93 (MQ=255)
        aTGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGAc                                                                                   >  2:2444829/1‑93 (MQ=255)
         tGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACg                                                                                  >  1:6449857/1‑93 (MQ=255)
           gATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCt                                                                                <  1:3528177/93‑1 (MQ=255)
             ttGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCttt                                                                              <  2:6169927/93‑1 (MQ=255)
              tGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCtttt                                                                             <  1:4486564/93‑1 (MQ=255)
                  ttACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGGTGAATGGTTCTCGATTGTGCGCTGGACGAttttatcc                                                                         >  1:3313386/1‑89 (MQ=255)
                  ttACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGcc                                                                         >  2:5875106/1‑93 (MQ=255)
                    aCCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCat                                                                       <  2:3246569/93‑1 (MQ=255)
                     ccGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatg                                                                      >  1:1510179/1‑93 (MQ=255)
                     ccGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatg                                                                      >  2:5395416/1‑93 (MQ=255)
                      cGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgc                                                                     >  1:1564247/1‑93 (MQ=255)
                      cGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgc                                                                     <  2:6476800/93‑1 (MQ=255)
                        gAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgctg                                                                   <  1:3130582/93‑1 (MQ=255)
                        gAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgctg                                                                   >  1:81367/1‑93 (MQ=255)
                         aaGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgctga                                                                  >  2:4291218/1‑93 (MQ=255)
                          aGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCatgctgaa                                                                 <  2:5687592/93‑1 (MQ=255)
                            ttATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgctgaatg                                                               <  1:5151450/93‑1 (MQ=255)
                             tATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgctgaatgc                                                              <  2:2912545/93‑1 (MQ=255)
                               tctcAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgctgaatgctg                                                            >  1:1101043/1‑93 (MQ=255)
                                ctcAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCatgctgaatgctga                                                           <  2:5929641/93‑1 (MQ=255)
                                 tcAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCAtgctgaatgctgaa                                                          <  2:1453878/93‑1 (MQ=255)
                                 tcAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCAtgctgaatgctgaa                                                          <  1:6410591/93‑1 (MQ=255)
                                   aaaaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAaga                                                        <  1:998639/93‑1 (MQ=255)
                                   aaaaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAaga                                                        >  2:3333058/1‑93 (MQ=255)
                                   aaaaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAaga                                                        >  2:4687849/1‑93 (MQ=255)
                                    aaaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAagag                                                       >  2:2732546/1‑93 (MQ=255)
                                    aaaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAagag                                                       >  2:5311408/1‑93 (MQ=255)
                                    aaaGAACCGCTTGGTCCGGTAGTTCGTCCTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAagag                                                       <  1:6219139/93‑1 (MQ=255)
                                     aaGACCCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAgaga                                                      <  2:4693115/93‑1 (MQ=255)
                                     aaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAgaga                                                      <  2:5189813/93‑1 (MQ=255)
                                     aaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAgaga                                                      >  2:790447/1‑93 (MQ=255)
                                     aaGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAgaga                                                      >  1:2968221/1‑93 (MQ=255)
                                      aGAACCGCTTTGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGAt                                                     <  1:2083485/93‑1 (MQ=255)
                                      aGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGAt                                                     <  1:2506555/93‑1 (MQ=255)
                                       gAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATg                                                    <  1:129125/93‑1 (MQ=255)
                                       gAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATg                                                    >  1:2449044/1‑93 (MQ=255)
                                          ccGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGc                                                 <  2:2954450/93‑1 (MQ=255)
                                            gCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCAt                                               >  2:3917971/1‑93 (MQ=255)
                                            gCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCAt                                               <  1:829333/93‑1 (MQ=255)
                                            gCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCAt                                               >  1:4767292/1‑93 (MQ=255)
                                             cTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATc                                              >  1:3424443/1‑93 (MQ=255)
                                             cTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATc                                              >  1:3758047/1‑93 (MQ=255)
                                              ttGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCa                                             <  1:2666786/93‑1 (MQ=255)
                                              ttGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCa                                             >  1:4519704/1‑93 (MQ=255)
                                               tGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCaa                                            <  1:4005903/93‑1 (MQ=255)
                                                 gTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAAtt                                          >  1:4416250/1‑93 (MQ=255)
                                                 gTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAAtt                                          <  2:2063157/93‑1 (MQ=255)
                                                  tCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTTCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTc                                         <  1:2176327/93‑1 (MQ=255)
                                                  tCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTc                                         <  1:688715/93‑1 (MQ=255)
                                                   ccGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTcc                                        <  2:459447/93‑1 (MQ=255)
                                                   ccGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTcc                                        >  1:3801530/1‑93 (MQ=255)
                                                     ggTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCTATGCTGAATGCTGAAGAGATGGGCATCAATTCCCa                                      <  2:3859178/93‑1 (MQ=255)
                                                     ggTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCa                                      <  2:1787915/93‑1 (MQ=255)
                                                     ggTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCa                                      >  2:3662327/1‑93 (MQ=255)
                                                     ggTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCa                                      <  2:2326498/93‑1 (MQ=255)
                                                       tAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGa                                    >  1:356616/1‑93 (MQ=255)
                                                         gTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAAc                                  <  1:3577554/93‑1 (MQ=255)
                                                            cgtcgtGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTc                               <  1:6462655/93‑1 (MQ=255)
                                                             gtcgtGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCg                              >  2:33659/1‑93 (MQ=255)
                                                             gtcgtGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCg                              >  1:5303925/1‑93 (MQ=255)
                                                              tcgtGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGa                             <  2:714753/93‑1 (MQ=255)
                                                               cgtGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCGGAAGAGATGGGCATCAATTCCCAGAACGTCGAt                            <  2:1810957/93‑1 (MQ=255)
                                                                gtGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATg                           >  2:4903943/1‑93 (MQ=255)
                                                                 tGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGa                          >  2:1316049/1‑93 (MQ=255)
                                                                  ggCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGaa                         <  1:369408/93‑1 (MQ=255)
                                                                   gCGGTGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGaaa                        >  2:2145708/1‑93 (MQ=255)
                                                                   gCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGaaa                        <  1:2833319/93‑1 (MQ=255)
                                                                     gatgatGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCTGAACGTCGATGaaaaa                      >  2:1297364/1‑93 (MQ=255)
                                                                     gatgatGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGaaaaa                      <  2:3153677/93‑1 (MQ=255)
                                                                     gatgatGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGaaaaa                      <  1:4697321/93‑1 (MQ=255)
                                                                      atgatgAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAg                     <  1:3594008/93‑1 (MQ=255)
                                                                       tgatgaATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAgg                    >  2:5028079/1‑92 (MQ=255)
                                                                       tgatgaATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGc                    >  1:1858920/1‑93 (MQ=255)
                                                                       tgatgaATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGc                    >  2:6183248/1‑93 (MQ=255)
                                                                       tgatgaATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGc                    <  1:2741952/93‑1 (MQ=255)
                                                                       tgatgaATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGc                    <  2:4859371/93‑1 (MQ=255)
                                                                        gatgaATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCg                   <  2:3976604/93‑1 (MQ=255)
                                                                           gaATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCt                >  2:154975/1‑93 (MQ=255)
                                                                             aTGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTaa              <  2:2359100/93‑1 (MQ=255)
                                                                              tGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAAt             >  2:2967973/1‑93 (MQ=255)
                                                                              tGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAAt             <  1:5479010/93‑1 (MQ=255)
                                                                              tGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAAt             <  2:4465648/93‑1 (MQ=255)
                                                                               ggTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATc            <  2:115847/93‑1 (MQ=255)
                                                                                gTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATcc           <  1:4972969/93‑1 (MQ=255)
                                                                                gTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATcc           <  1:6366575/93‑1 (MQ=255)
                                                                                   ttcGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGc        <  1:4896329/92‑1 (MQ=255)
                                                                                        ttGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGc   >  2:3075690/1‑93 (MQ=255)
                                                                                        ttGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGc   >  2:4023869/1‑93 (MQ=255)
                                                                                         tgtgCGCTGGACGCTTTTCGCCATGCTGAAGGCTGAAGAGATCGGCATCAATTCCCAGACCGTCGATGAAAAAGCGGCTAATCCAGCAACGcc  <  2:3483788/93‑1 (MQ=255)
                                                                                            |                                                                                         
CCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGCC  >  NC_000913/3417311‑3417492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGCCTGA  >  NC_000913/3417310‑3417495
                                                                                             |                                                                                            
CCCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTG                                                                                              >  SRR4302537.2250095/1‑94 (MQ=60)
CCCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTG                                                                                              >  SRR4302537.1745963/1‑94 (MQ=60)
 CCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGC                                                                                             >  SRR4302537.559037/1‑94 (MQ=60)
 CCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTAC                                                                                             <  SRR4302537.3524633/94‑1 (MQ=60)
  CAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCG                                                                                            <  SRR4302537.346138/94‑1 (MQ=60)
  CAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCG                                                                                            >  SRR4302537.3702996/1‑94 (MQ=60)
  CAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCG                                                                                            >  SRR4302537.5659459/1‑94 (MQ=60)
   AGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGC                                                                                           >  SRR4302537.4192828/1‑94 (MQ=60)
    GCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCT                                                                                          <  SRR4302537.5288717/94‑1 (MQ=60)
    GCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCT                                                                                          <  SRR4302537.5921878/94‑1 (MQ=60)
     CTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTG                                                                                         >  SRR4302537.3090450/1‑94 (MQ=60)
     CTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTG                                                                                         >  SRR4302537.856857/1‑94 (MQ=60)
      TGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGG                                                                                        >  SRR4302537.4259292/1‑94 (MQ=60)
      TGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGG                                                                                        >  SRR4302537.1783531/1‑94 (MQ=60)
      TGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGCTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGG                                                                                        >  SRR4302537.2882569/1‑94 (MQ=60)
       GAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGA                                                                                       <  SRR4302537.4572795/94‑1 (MQ=60)
       GAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGA                                                                                       >  SRR4302537.5812001/1‑94 (MQ=60)
       GAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGA                                                                                       >  SRR4302537.5290317/1‑94 (MQ=60)
        AATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGAC                                                                                      <  SRR4302537.5556150/94‑1 (MQ=60)
        AATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGAC                                                                                      <  SRR4302537.2153273/94‑1 (MQ=60)
         ATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACG                                                                                     <  SRR4302537.889135/94‑1 (MQ=60)
         ATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACG                                                                                     >  SRR4302537.6493598/1‑94 (MQ=60)
          TGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGC                                                                                    >  SRR4302537.478215/1‑94 (MQ=60)
           GGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCT                                                                                   <  SRR4302537.815598/94‑1 (MQ=60)
            GATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTT                                                                                  <  SRR4302537.3553130/94‑1 (MQ=60)
             ATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTT                                                                                 >  SRR4302537.4944880/1‑94 (MQ=60)
               TGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTC                                                                               <  SRR4302537.4517602/94‑1 (MQ=60)
               TGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTC                                                                               <  SRR4302537.2312397/94‑1 (MQ=60)
               TGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGAGCTGGACGCTTTTC                                                                               >  SRR4302537.2123966/1‑94 (MQ=60)
                  CTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGGTGAATGGTTCTCGATTGTGCGCTGGACGattttatcc                                                                            >  SRR4302537.3336825/1‑85 (MQ=60)
                  CTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCC                                                                            >  SRR4302537.2522297/1‑94 (MQ=60)
                  CTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCC                                                                            >  SRR4302537.2536041/1‑94 (MQ=60)
                  CTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCC                                                                            <  SRR4302537.3817638/94‑1 (MQ=60)
                  CTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCC                                                                            >  SRR4302537.3772102/1‑94 (MQ=60)
                  CTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCC                                                                            <  SRR4302537.2000504/94‑1 (MQ=60)
                   TTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCA                                                                           >  SRR4302537.3279536/1‑94 (MQ=60)
                    TACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCAT                                                                          <  SRR4302537.5868349/94‑1 (MQ=60)
                    TACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCAT                                                                          >  SRR4302537.2169922/1‑94 (MQ=60)
                     ACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATG                                                                         >  SRR4302537.1520738/1‑94 (MQ=60)
                      CCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGC                                                                        >  SRR4302537.1575157/1‑94 (MQ=60)
                        GGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTG                                                                      >  SRR4302537.81967/1‑94 (MQ=60)
                        GGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTG                                                                      <  SRR4302537.5308191/94‑1 (MQ=60)
                         GAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGA                                                                     <  SRR4302537.183763/94‑1 (MQ=60)
                         GAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGA                                                                     <  SRR4302537.3152531/94‑1 (MQ=60)
                            GTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCACTGGACGCTTTTCGCgttgcatcatg                                                                  >  SRR4302537.1576000/1‑83 (MQ=60)
                             TTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGC                                                                 <  SRR4302537.5186946/94‑1 (MQ=60)
                               ATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTG                                                               >  SRR4302537.1108654/1‑94 (MQ=60)
                                 TTCAAAAGAACCGCTTGTTCCAGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAA                                                             <  SRR4302537.356598/94‑1 (MQ=60)
                                  TCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAG                                                            <  SRR4302537.4511405/94‑1 (MQ=60)
                                  TCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAG                                                            <  SRR4302537.6454208/94‑1 (MQ=60)
                                  TCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAG                                                            >  SRR4302537.3619021/1‑94 (MQ=60)
                                   CAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGA                                                           >  SRR4302537.3384201/1‑94 (MQ=60)
                                   CAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGA                                                           >  SRR4302537.4328894/1‑94 (MQ=60)
                                    AAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAG                                                          <  SRR4302537.1005533/94‑1 (MQ=60)
                                    AAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAG                                                          <  SRR4302537.3694928/94‑1 (MQ=60)
                                    AAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAG                                                          <  SRR4302537.5161232/94‑1 (MQ=60)
                                     AAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGA                                                         >  SRR4302537.1398242/1‑94 (MQ=60)
                                     AAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGA                                                         <  SRR4302537.2766198/94‑1 (MQ=60)
                                     AAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGA                                                         >  SRR4302537.2989032/1‑94 (MQ=60)
                                     AAAGAACCGCTTGGTCCGGTAGTTCGTCCTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGA                                                         <  SRR4302537.6261957/94‑1 (MQ=60)
                                       AGAACCGCTTTGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATG                                                       <  SRR4302537.2098165/94‑1 (MQ=60)
                                       AGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATG                                                       >  SRR4302537.2466272/1‑94 (MQ=60)
                                       AGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATG                                                       <  SRR4302537.2524186/94‑1 (MQ=60)
                                        GAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGG                                                      <  SRR4302537.130042/94‑1 (MQ=60)
                                         AACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGG                                                     <  SRR4302537.3591552/94‑1 (MQ=60)
                                         AACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGG                                                     >  SRR4302537.6294765/1‑94 (MQ=60)
                                            CGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCAT                                                  >  SRR4302537.4800214/1‑94 (MQ=60)
                                             GCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATC                                                 >  SRR4302537.3784433/1‑94 (MQ=60)
                                             GCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATC                                                 <  SRR4302537.835009/94‑1 (MQ=60)
                                             GCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATC                                                 >  SRR4302537.3448683/1‑94 (MQ=60)
                                             GCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATC                                                 >  SRR4302537.1507399/1‑94 (MQ=60)
                                              CTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCA                                                >  SRR4302537.4550964/1‑94 (MQ=60)
                                               TTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAA                                               >  SRR4302537.6486181/1‑94 (MQ=60)
                                               TTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAA                                               <  SRR4302537.2685415/94‑1 (MQ=60)
                                                TGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAAT                                              <  SRR4302537.4033938/94‑1 (MQ=60)
                                                TGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAAT                                              <  SRR4302537.1775719/94‑1 (MQ=60)
                                                 GGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATT                                             >  SRR4302537.4446739/1‑94 (MQ=60)
                                                  GTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTC                                            <  SRR4302537.4829154/94‑1 (MQ=60)
                                                   TCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTTCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCC                                           <  SRR4302537.2191666/94‑1 (MQ=60)
                                                   TCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCC                                           <  SRR4302537.6515962/94‑1 (MQ=60)
                                                   TCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCC                                           <  SRR4302537.693433/94‑1 (MQ=60)
                                                   TCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCC                                           >  SRR4302537.3828201/1‑94 (MQ=60)
                                                    CCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCTC                                          >  SRR4302537.6438797/1‑94 (MQ=60)
                                                    CCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCC                                          <  SRR4302537.2741876/94‑1 (MQ=60)
                                                     CGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCA                                         >  SRR4302537.3018850/1‑94 (MQ=60)
                                                       GTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGA                                       >  SRR4302537.4581091/1‑94 (MQ=60)
                                                       GTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGA                                       >  SRR4302537.359093/1‑94 (MQ=60)
                                                        TAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAA                                      >  SRR4302537.4003062/1‑94 (MQ=60)
                                                         AGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAAC                                     <  SRR4302537.1778668/94‑1 (MQ=60)
                                                          GTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACG                                    >  SRR4302537.452722/1‑94 (MQ=60)
                                                          GTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACG                                    <  SRR4302537.3520692/94‑1 (MQ=60)
                                                          GTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACG                                    <  SRR4302537.2899639/94‑1 (MQ=60)
                                                          GTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACG                                    <  SRR4302537.3602775/94‑1 (MQ=60)
                                                           TTCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGT                                   >  SRR4302537.5456170/1‑94 (MQ=60)
                                                            TCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTC                                  <  SRR4302537.816076/94‑1 (MQ=60)
                                                            TCGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTC                                  >  SRR4302537.6466085/1‑94 (MQ=60)
                                                             CGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCG                                 <  SRR4302537.6506441/94‑1 (MQ=60)
                                                             CGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCG                                 >  SRR4302537.2150927/1‑94 (MQ=60)
                                                             CGTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCG                                 >  SRR4302537.5340381/1‑94 (MQ=60)
                                                              GTCGTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGA                                >  SRR4302537.5192315/1‑94 (MQ=60)
                                                                 GTGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGA                             <  SRR4302537.6505781/94‑1 (MQ=60)
                                                                  TGGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAA                            <  SRR4302537.6141717/94‑1 (MQ=60)
                                                                   GGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAA                           >  SRR4302537.2273529/1‑94 (MQ=60)
                                                                   GGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAA                           <  SRR4302537.371969/94‑1 (MQ=60)
                                                                   GGCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAA                           <  SRR4302537.4860130/94‑1 (MQ=60)
                                                                    GCGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAA                          <  SRR4302537.2853130/94‑1 (MQ=60)
                                                                     CGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAA                         >  SRR4302537.853303/1‑94 (MQ=60)
                                                                     CGATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAA                         >  SRR4302537.3137158/1‑94 (MQ=60)
                                                                      GATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAG                        <  SRR4302537.2709431/94‑1 (MQ=60)
                                                                      GATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAG                        <  SRR4302537.3141726/94‑1 (MQ=60)
                                                                      GATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAG                        <  SRR4302537.4729819/94‑1 (MQ=60)
                                                                       ATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGC                       >  SRR4302537.1871868/1‑94 (MQ=60)
                                                                       ATGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGC                       <  SRR4302537.3619344/94‑1 (MQ=60)
                                                                        TGATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCG                      <  SRR4302537.2761081/94‑1 (MQ=60)
                                                                         GATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGG                     <  SRR4302537.3738300/94‑1 (MQ=60)
                                                                         GATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGG                     <  SRR4302537.5555820/94‑1 (MQ=60)
                                                                         GATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGG                     <  SRR4302537.1558240/94‑1 (MQ=60)
                                                                         GATGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGG                     >  SRR4302537.3593394/1‑94 (MQ=60)
                                                                           TGAATGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCT                   <  SRR4302537.331018/94‑1 (MQ=60)
                                                                               TGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATC               <  SRR4302537.5516417/94‑1 (MQ=60)
                                                                               TGGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATC               <  SRR4302537.5450341/94‑1 (MQ=60)
                                                                                GGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCC              <  SRR4302537.842434/94‑1 (MQ=60)
                                                                                GGTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCC              >  SRR4302537.4494411/1‑94 (MQ=60)
                                                                                 GTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCA             <  SRR4302537.6409997/94‑1 (MQ=60)
                                                                                 GTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCA             <  SRR4302537.5007243/94‑1 (MQ=60)
                                                                                 GTTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCA             <  SRR4302537.4310841/94‑1 (MQ=60)
                                                                                  TTCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAG            <  SRR4302537.399966/94‑1 (MQ=60)
                                                                                   TCTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGC           >  SRR4302537.2067824/1‑94 (MQ=60)
                                                                                    TTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCA          <  SRR4302537.4930132/94‑1 (MQ=60)
                                                                                    CTCGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCA          <  SRR4302537.4978059/94‑1 (MQ=60)
                                                                                    CTCGATTGTGCGCTCGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCA          <  SRR4302537.5550756/94‑1 (MQ=60)
                                                                                      CGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAAC        >  SRR4302537.2632573/1‑94 (MQ=60)
                                                                                      CGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAAC        <  SRR4302537.6498651/94‑1 (MQ=60)
                                                                                      CGATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATACAGCAAC        >  SRR4302537.5593123/1‑94 (MQ=60)
                                                                                       GATTGTGCGCTGGACGCTTTTCGCGATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACG       <  SRR4302537.823187/94‑1 (MQ=60)
                                                                                       GATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACG       >  SRR4302537.6352800/1‑94 (MQ=60)
                                                                                       GATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACG       <  SRR4302537.1264011/94‑1 (MQ=60)
                                                                                       GATTGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACG       <  SRR4302537.992282/94‑1 (MQ=60)
                                                                                          TGTGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGCCT    >  SRR4302537.1480722/1‑94 (MQ=60)
                                                                                            TGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGCCTGA  <  SRR4302537.1405578/94‑1 (MQ=60)
                                                                                            TGCGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGCCTGA  <  SRR4302537.4935374/94‑1 (MQ=60)
                                                                                             |                                                                                            
CCCAGCTGAATGGATTGTCTTACCGGAAGTTATCTCAAAAGAACCGCTTGGTCCGGTAGTTCGTCGTGGCGATGATGAATGGTTCTCGATTGTACGCTGGACGCTTTTCGCCATGCTGAATGCTGAAGAGATGGGCATCAATTCCCAGAACGTCGATGAAAAAGCGGCTAATCCAGCAACGCCTGA  >  NC_000913/3417310‑3417495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑