Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,621,249 G→A G69R (GGA→AGA)  yhhH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,621,2490GA95.4% 907.8 / 17.0 261G69R (GGA→AGA) yhhHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (8/4);  new base A (130/119);  total (138/123)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

Only 100 of 264 total aligned reads displayed.

GAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAA  >  NC_000913/3621159‑3621338
                                                                                          |                                                                                         
gAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGa                                                                                         <  2:5382698/93‑1 (MQ=255)
gAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGa                                                                                         >  2:2605934/1‑93 (MQ=255)
gAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGa                                                                                         <  2:1567088/93‑1 (MQ=255)
 aaaGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGAc                                                                                        >  1:821453/1‑93 (MQ=255)
 aaaGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGAc                                                                                        >  2:1393556/1‑93 (MQ=255)
    gTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAgg                                                                                     >  2:1219819/1‑93 (MQ=255)
    gTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAgg                                                                                     >  2:4077282/1‑93 (MQ=255)
    gTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAgg                                                                                     <  1:1490450/93‑1 (MQ=255)
     tGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGa                                                                                    <  2:3796658/93‑1 (MQ=255)
       aaGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATg                                                                                  >  1:3091580/1‑93 (MQ=255)
        aGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGc                                                                                 >  1:3859124/1‑93 (MQ=255)
         ggTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGcc                                                                                <  1:4402702/93‑1 (MQ=255)
          gTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCg                                                                               >  1:5834738/1‑93 (MQ=255)
           tAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGc                                                                              >  2:563589/1‑93 (MQ=255)
           tAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGc                                                                              >  1:4809213/1‑93 (MQ=255)
               tataCCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaa                                                                          >  2:1741397/1‑93 (MQ=255)
               tataCCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaa                                                                          >  1:64198/1‑93 (MQ=255)
               tataCCGTTAGAAATTTGGGTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaa                                                                          >  2:6244220/1‑93 (MQ=255)
                ataCCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaag                                                                         <  1:1094221/93‑1 (MQ=255)
                  aCCGTTAGAAATTTGGGTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaagaa                                                                       >  2:5521098/1‑93 (MQ=255)
                   ccGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaagaag                                                                      >  1:4922566/1‑93 (MQ=255)
                    cGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaagaaga                                                                     <  1:3801827/93‑1 (MQ=255)
                    cGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCagaagaaga                                                                     <  2:2029626/93‑1 (MQ=255)
                      ttAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGaaa                                                                   <  1:2214618/93‑1 (MQ=255)
                       tAGAAATTTGGTTAAAAATACAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGaaaa                                                                  <  1:4302359/93‑1 (MQ=255)
                       tAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGaaaa                                                                  >  1:3616135/1‑93 (MQ=255)
                       tAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGaaaa                                                                  >  1:4520637/1‑93 (MQ=255)
                        aGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGaaaaa                                                                 <  2:3593069/93‑1 (MQ=255)
                        aGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGaaaaa                                                                 <  1:2171869/93‑1 (MQ=255)
                         gAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAAc                                                                <  1:4684147/93‑1 (MQ=255)
                         gAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAAc                                                                <  1:2409022/93‑1 (MQ=255)
                           aaTTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCa                                                              >  2:2933246/1‑93 (MQ=255)
                           aaTTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCa                                                              <  1:4219255/93‑1 (MQ=255)
                           aaTTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCa                                                              <  1:3710488/93‑1 (MQ=255)
                            aTTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCAt                                                             <  1:289803/93‑1 (MQ=255)
                            aTTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCAt                                                             >  1:5855615/1‑93 (MQ=255)
                             tttGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATa                                                            <  1:6176109/93‑1 (MQ=255)
                              ttGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATAc                                                           <  2:444475/93‑1 (MQ=255)
                               tGGTTAAAAATAAAGCGATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATTCg                                                          <  2:5885455/93‑1 (MQ=255)
                                 gTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGaa                                                        >  1:1185093/1‑93 (MQ=255)
                                  ttAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGaaa                                                       <  2:2816047/93‑1 (MQ=255)
                                    aaaaaTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATc                                                     <  1:236926/93‑1 (MQ=255)
                                     aaaaTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCa                                                    >  1:3995171/1‑93 (MQ=255)
                                     aaaaTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCa                                                    >  1:5607098/1‑93 (MQ=255)
                                      aaaTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCAc                                                   <  1:1357195/93‑1 (MQ=255)
                                      aaaTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCAc                                                   >  1:3254056/1‑93 (MQ=255)
                                      aaaTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCAc                                                   <  1:6034847/93‑1 (MQ=255)
                                       aaTAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACt                                                  >  2:4505446/1‑93 (MQ=255)
                                       aaTAAACCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACt                                                  <  2:1039408/93‑1 (MQ=255)
                                         tAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATATACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGa                                                <  1:3464382/93‑1 (MQ=255)
                                         tAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGa                                                >  1:6125909/1‑93 (MQ=255)
                                         tAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGa                                                <  1:2614434/93‑1 (MQ=255)
                                          aaaGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAg                                               >  2:4926356/1‑93 (MQ=255)
                                           aaGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGt                                              <  1:1276158/93‑1 (MQ=255)
                                             gCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTa                                            <  2:4970150/93‑1 (MQ=255)
                                              cTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTaa                                           >  1:994095/1‑93 (MQ=255)
                                              cTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTaa                                           >  1:354780/1‑93 (MQ=255)
                                                ataGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTaaca                                         >  2:5821934/1‑93 (MQ=255)
                                                ataGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTaaca                                         >  2:2275699/1‑93 (MQ=255)
                                                  aGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTaacaac                                       >  1:5420332/1‑93 (MQ=255)
                                                   gCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAacaaca                                      >  2:820450/1‑93 (MQ=255)
                                                   gCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAacaaca                                      <  2:288882/93‑1 (MQ=255)
                                                     aTTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAg                                    <  1:2818950/93‑1 (MQ=255)
                                                     aTTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCAt                                                             <  2:4065065/68‑1 (MQ=255)
                                                     aTTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAg                                    >  1:2538488/1‑93 (MQ=255)
                                                      ttGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGt                                   <  2:4177179/93‑1 (MQ=255)
                                                       tGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGtt                                  >  2:5355617/1‑93 (MQ=255)
                                                       tGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGtt                                  <  1:4738828/93‑1 (MQ=255)
                                                        ggAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTg                                 <  2:3367396/93‑1 (MQ=255)
                                                         gAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTgg                                <  1:5736208/93‑1 (MQ=255)
                                                         gAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTgg                                >  1:4096150/1‑93 (MQ=255)
                                                           aTTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgt                              >  1:1579074/1‑93 (MQ=255)
                                                           aTTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgt                              >  1:6303505/1‑93 (MQ=255)
                                                           aTTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgt                              <  2:4823385/93‑1 (MQ=255)
                                                           aTTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgt                              <  2:6105708/93‑1 (MQ=255)
                                                            ttAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgtt                             >  2:5856051/1‑93 (MQ=255)
                                                             tAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgttg                            <  2:4574657/93‑1 (MQ=255)
                                                              aGCTGAGATATATGTTAAGAATCGTAATAGACAGGATGCCGCAGAAGAAGAAAAACCATACTAAATCACTGAGTTAACAACAAGTTGGgttgt                           <  2:6427184/93‑1 (MQ=255)
                                                               gCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgttgtt                          <  2:4707796/93‑1 (MQ=255)
                                                               gCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGgttgtt                          >  2:961367/1‑93 (MQ=255)
                                                                 tGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGa                        >  1:3238116/1‑93 (MQ=255)
                                                                  gagaTATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGaa                       >  2:1113787/1‑93 (MQ=255)
                                                                   agaTATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAg                      >  1:1048249/1‑93 (MQ=255)
                                                                   agaTATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAg                      <  1:4589483/93‑1 (MQ=255)
                                                                     atatatGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGt                    >  1:3017084/1‑93 (MQ=255)
                                                                      tatatGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTa                   <  2:2167733/93‑1 (MQ=255)
                                                                      tatatGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTa                   >  1:210714/1‑93 (MQ=255)
                                                                        tatGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTAcc                 >  2:2295650/1‑93 (MQ=255)
                                                                        tatGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTAcc                 >  1:4933274/1‑93 (MQ=255)
                                                                         atGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCa                >  2:5144671/1‑93 (MQ=255)
                                                                         atGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCa                >  2:3481121/1‑93 (MQ=255)
                                                                          tGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCAt               >  1:1729567/1‑93 (MQ=255)
                                                                          tGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCAt               >  2:3244199/1‑93 (MQ=255)
                                                                            ttAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGAGTTGTTGAAGGTACCATTc             <  2:6307769/93‑1 (MQ=255)
                                                                             tAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCa            >  2:1790199/1‑93 (MQ=255)
                                                                                   tCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGa      <  2:2114320/93‑1 (MQ=255)
                                                                                      ttATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCa   >  1:565821/1‑93 (MQ=255)
                                                                                      ttATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCa   >  1:4256610/1‑93 (MQ=255)
                                                                                      ttATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCa   <  2:736706/93‑1 (MQ=255)
                                                                                      ttATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCa   >  2:3198116/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAA  >  NC_000913/3621159‑3621338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTG  >  NC_000913/3621156‑3621342
                                                                                             |                                                                                             
TATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATA                                                                                               <  SRR4302537.5209348/94‑1 (MQ=60)
TATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATA                                                                                               <  SRR4302537.1793924/94‑1 (MQ=60)
TATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATA                                                                                               <  SRR4302537.377786/94‑1 (MQ=60)
TATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATA                                                                                               >  SRR4302537.741731/1‑94 (MQ=60)
 ATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAG                                                                                              >  SRR4302537.5146238/1‑94 (MQ=60)
 ATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAG                                                                                              <  SRR4302537.4926550/94‑1 (MQ=60)
 ATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAG                                                                                              >  SRR4302537.2460695/1‑94 (MQ=60)
 ATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAG                                                                                              <  SRR4302537.1588912/94‑1 (MQ=60)
 ATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAG                                                                                              <  SRR4302537.3577646/94‑1 (MQ=60)
   GAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGAC                                                                                            >  SRR4302537.827091/1‑94 (MQ=60)
    AAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACA                                                                                           <  SRR4302537.22110/94‑1 (MQ=60)
    AAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACA                                                                                           >  SRR4302537.6392349/1‑94 (MQ=60)
    AAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACA                                                                                           <  SRR4302537.1500513/94‑1 (MQ=60)
    AAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACA                                                                                           >  SRR4302537.5927594/1‑94 (MQ=60)
      AGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGG                                                                                         <  SRR4302537.4751407/94‑1 (MQ=60)
       GTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGA                                                                                        <  SRR4302537.1500828/94‑1 (MQ=60)
       GTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGA                                                                                        <  SRR4302537.624007/94‑1 (MQ=60)
        TGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGAT                                                                                       >  SRR4302537.4794613/1‑94 (MQ=60)
        TGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGAT                                                                                       <  SRR4302537.2208229/94‑1 (MQ=60)
         GAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATG                                                                                      <  SRR4302537.3183930/94‑1 (MQ=60)
         GAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATG                                                                                      >  SRR4302537.3113145/1‑94 (MQ=60)
          AAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGC                                                                                     >  SRR4302537.3886140/1‑94 (MQ=60)
           AGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCC                                                                                    >  SRR4302537.3641850/1‑94 (MQ=60)
            GGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCG                                                                                   <  SRR4302537.4433081/94‑1 (MQ=60)
            GGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCG                                                                                   >  SRR4302537.5874729/1‑94 (MQ=60)
            GGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCG                                                                                   <  SRR4302537.134777/94‑1 (MQ=60)
             GTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGC                                                                                  <  SRR4302537.4270469/94‑1 (MQ=60)
             GTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGC                                                                                  >  SRR4302537.4842408/1‑94 (MQ=60)
              TAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCA                                                                                 <  SRR4302537.3268376/94‑1 (MQ=60)
                 ATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAA                                                                              >  SRR4302537.64676/1‑94 (MQ=60)
                 ATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAA                                                                              >  SRR4302537.5880921/1‑94 (MQ=60)
                   ATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGA                                                                            <  SRR4302537.1101780/94‑1 (MQ=60)
                    TACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAA                                                                           <  SRR4302537.4667845/94‑1 (MQ=60)
                    TACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAA                                                                           <  SRR4302537.4285932/94‑1 (MQ=60)
                     ACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAG                                                                          >  SRR4302537.4956514/1‑94 (MQ=60)
                      CCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGA                                                                         >  SRR4302537.4523359/1‑94 (MQ=60)
                      CCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGA                                                                         <  SRR4302537.496342/94‑1 (MQ=60)
                       CGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAA                                                                        <  SRR4302537.3828498/94‑1 (MQ=60)
                         TTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAA                                                                      >  SRR4302537.4551906/1‑94 (MQ=60)
                         TTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAA                                                                      >  SRR4302537.3641632/1‑94 (MQ=60)
                         TTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAA                                                                      <  SRR4302537.2230238/94‑1 (MQ=60)
                          TAGAAATTTGGTTAAAAATACAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAA                                                                     <  SRR4302537.4332257/94‑1 (MQ=60)
                          TAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAA                                                                     <  SRR4302537.4758358/94‑1 (MQ=60)
                          TAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATCAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAA                                                                     >  SRR4302537.3161086/1‑94 (MQ=60)
                           AGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAAC                                                                    >  SRR4302537.3474985/1‑94 (MQ=60)
                           AGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAAC                                                                    <  SRR4302537.5811422/94‑1 (MQ=60)
                           AGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAAC                                                                    <  SRR4302537.2840775/94‑1 (MQ=60)
                           AGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAAC                                                                    <  SRR4302537.867943/94‑1 (MQ=60)
                           AGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAAC                                                                    <  SRR4302537.5759953/94‑1 (MQ=60)
                           AGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAAC                                                                    <  SRR4302537.2187178/94‑1 (MQ=60)
                            GAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACC                                                                   <  SRR4302537.4716546/94‑1 (MQ=60)
                            GAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACC                                                                   <  SRR4302537.2425981/94‑1 (MQ=60)
                             AAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCA                                                                  >  SRR4302537.3844466/1‑94 (MQ=60)
                             AAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCA                                                                  <  SRR4302537.2754448/94‑1 (MQ=60)
                             AAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCA                                                                  >  SRR4302537.6395124/1‑94 (MQ=60)
                             AAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCA                                                                  >  SRR4302537.5800583/1‑94 (MQ=60)
                             AAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCA                                                                  >  SRR4302537.1077457/1‑94 (MQ=60)
                              AATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCAT                                                                 <  SRR4302537.3736570/94‑1 (MQ=60)
                              AATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCAT                                                                 >  SRR4302537.5895778/1‑94 (MQ=60)
                              AATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCAT                                                                 <  SRR4302537.4248655/94‑1 (MQ=60)
                               ATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATA                                                                <  SRR4302537.291821/94‑1 (MQ=60)
                               ATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATA                                                                <  SRR4302537.1342105/94‑1 (MQ=60)
                               ATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCAGCAGAAGAAGAAAAACCATA                                                                >  SRR4302537.4330530/1‑94 (MQ=60)
                                TTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATAC                                                               <  SRR4302537.1219795/94‑1 (MQ=60)
                                TTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATAC                                                               <  SRR4302537.6218646/94‑1 (MQ=60)
                                 TTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACG                                                              <  SRR4302537.3940480/94‑1 (MQ=60)
                                  tcttccgatctattccgtTAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGA                                                             <  SRR4302537.2896151/76‑1 (MQ=60)
                                   GGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAA                                                            <  SRR4302537.171782/94‑1 (MQ=60)
                                   GGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAA                                                            >  SRR4302537.1193330/1‑94 (MQ=60)
                                     TTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAAT                                                          <  SRR4302537.761355/94‑1 (MQ=60)
                                      TAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATC                                                         >  SRR4302537.4165596/1‑94 (MQ=60)
                                      TAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATC                                                         <  SRR4302537.2307541/94‑1 (MQ=60)
                                       AAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCA                                                        <  SRR4302537.238595/94‑1 (MQ=60)
                                       AAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCA                                                        >  SRR4302537.4023136/1‑94 (MQ=60)
                                       AAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCA                                                        >  SRR4302537.5645431/1‑94 (MQ=60)
                                        AAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCAC                                                       <  SRR4302537.5924141/94‑1 (MQ=60)
                                        AAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCAC                                                       >  SRR4302537.3277003/1‑94 (MQ=60)
                                         AAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACT                                                      <  SRR4302537.6076311/94‑1 (MQ=60)
                                         AAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACT                                                      >  SRR4302537.2452330/1‑94 (MQ=60)
                                         AAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACT                                                      <  SRR4302537.1366648/94‑1 (MQ=60)
                                           ATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGA                                                    >  SRR4302537.6168015/1‑94 (MQ=60)
                                           ATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGA                                                    >  SRR4302537.5522016/1‑94 (MQ=60)
                                            TAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATATACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAG                                                   <  SRR4302537.3488878/94‑1 (MQ=60)
                                            TAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAG                                                   <  SRR4302537.1119038/94‑1 (MQ=60)
                                            TAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAG                                                   <  SRR4302537.2632721/94‑1 (MQ=60)
                                             AAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGT                                                  >  SRR4302537.1461613/1‑94 (MQ=60)
                                              AAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTT                                                 >  SRR4302537.4371721/1‑94 (MQ=60)
                                              AAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTT                                                 <  SRR4302537.1285031/94‑1 (MQ=60)
                                                GCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAA                                               >  SRR4302537.357250/1‑94 (MQ=60)
                                                GCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAA                                               >  SRR4302537.1000961/1‑94 (MQ=60)
                                                 CTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAAC                                              <  SRR4302537.453432/94‑1 (MQ=60)
                                                 CTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAAC                                              <  SRR4302537.3781298/94‑1 (MQ=60)
                                                   ATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAA                                            >  SRR4302537.3496281/1‑94 (MQ=60)
                                                    TAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAAC                                           >  SRR4302537.5457268/1‑94 (MQ=60)
                                                     AGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACA                                          >  SRR4302537.965996/1‑94 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAG                                        <  SRR4302537.2527732/94‑1 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATAacggaatagatcggaagagcggtt                                        >  SRR4302537.4093468/1‑70 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAG                                        <  SRR4302537.433538/94‑1 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAG                                        <  SRR4302537.2695817/94‑1 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAG                                        >  SRR4302537.2556295/1‑94 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAG                                        >  SRR4302537.5657581/1‑94 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAG                                        >  SRR4302537.5513464/1‑94 (MQ=60)
                                                       CATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAG                                        <  SRR4302537.4637207/94‑1 (MQ=60)
                                                        ATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGT                                       <  SRR4302537.2838658/94‑1 (MQ=60)
                                                        ATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGT                                       <  SRR4302537.6107932/94‑1 (MQ=60)
                                                          TGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTG                                     <  SRR4302537.2165334/94‑1 (MQ=60)
                                                          TGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTG                                     <  SRR4302537.4771578/94‑1 (MQ=60)
                                                          TGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTG                                     >  SRR4302537.309564/1‑94 (MQ=60)
                                                          TGGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTG                                     >  SRR4302537.110437/1‑94 (MQ=60)
                                                           GGAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGG                                    >  SRR4302537.4124755/1‑94 (MQ=60)
                                                            GAATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGG                                   <  SRR4302537.5775484/94‑1 (MQ=60)
                                                             AATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGT                                  >  SRR4302537.1590109/1‑94 (MQ=60)
                                                             AATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGT                                  >  SRR4302537.6346671/1‑94 (MQ=60)
                                                              ATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTT                                 <  SRR4302537.306194/94‑1 (MQ=60)
                                                              ATTAGCTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTT                                 >  SRR4302537.4615845/1‑94 (MQ=60)
                                                                   CTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGA                            >  SRR4302537.2285648/1‑94 (MQ=60)
                                                                   CTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGA                            >  SRR4302537.3260942/1‑94 (MQ=60)
                                                                   CTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGA                            >  SRR4302537.4878314/1‑94 (MQ=60)
                                                                   CTGAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGA                            >  SRR4302537.3588697/1‑94 (MQ=60)
                                                                     GAGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAG                          >  SRR4302537.1055476/1‑94 (MQ=60)
                                                                      AGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGG                         <  SRR4302537.3407521/94‑1 (MQ=60)
                                                                      AGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGG                         >  SRR4302537.3520995/1‑94 (MQ=60)
                                                                      AGATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGG                         <  SRR4302537.4621222/94‑1 (MQ=60)
                                                                       GATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGT                        >  SRR4302537.3038114/1‑94 (MQ=60)
                                                                       GATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGT                        <  SRR4302537.3888576/94‑1 (MQ=60)
                                                                        ATATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTA                       >  SRR4302537.212224/1‑94 (MQ=60)
                                                                         TATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTAC                      >  SRR4302537.3647547/1‑94 (MQ=60)
                                                                          ATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACC                     <  SRR4302537.5707325/94‑1 (MQ=60)
                                                                          ATATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACC                     >  SRR4302537.4967299/1‑94 (MQ=60)
                                                                           TATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCA                    <  SRR4302537.2624157/94‑1 (MQ=60)
                                                                            ATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCAT                   >  SRR4302537.1741640/1‑94 (MQ=60)
                                                                            ATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCAT                   >  SRR4302537.4967816/1‑94 (MQ=60)
                                                                            ATGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCAT                   >  SRR4302537.6325589/1‑94 (MQ=60)
                                                                             TGTTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATT                  >  SRR4302537.4873834/1‑94 (MQ=60)
                                                                               TTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCA                >  SRR4302537.1650023/1‑94 (MQ=60)
                                                                               TTAAGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCA                <  SRR4302537.2406063/94‑1 (MQ=60)
                                                                                 AAGAATCGTTATAGACAGGATGCTGCAGAGCAAGAGAAGCCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACT              <  SRR4302537.1795260/94‑1 (MQ=60)
                                                                                  AGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTC             >  SRR4302537.3785007/1‑94 (MQ=60)
                                                                                  AGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTC             >  SRR4302537.6098069/1‑94 (MQ=60)
                                                                                  AGAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTC             >  SRR4302537.179760/1‑94 (MQ=60)
                                                                                   GAATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCA            <  SRR4302537.6206571/94‑1 (MQ=60)
                                                                                    AATCGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAG           >  SRR4302537.4136961/1‑94 (MQ=60)
                                                                                       CGTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACC        >  SRR4302537.1504159/1‑94 (MQ=60)
                                                                                        GTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCA       >  SRR4302537.4286195/1‑94 (MQ=60)
                                                                                        GTTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCA       >  SRR4302537.569709/1‑94 (MQ=60)
                                                                                         TTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAA      >  SRR4302537.5307372/1‑94 (MQ=60)
                                                                                         TTATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAA      >  SRR4302537.2998855/1‑94 (MQ=60)
                                                                                          TATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAA     >  SRR4302537.2798038/1‑94 (MQ=60)
                                                                                          TATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAA     >  SRR4302537.5638952/1‑94 (MQ=60)
                                                                                           ATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAT    <  SRR4302537.3774744/94‑1 (MQ=60)
                                                                                           ATAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAT    <  SRR4302537.4953322/94‑1 (MQ=60)
                                                                                            TAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATT   <  SRR4302537.6326773/94‑1 (MQ=60)
                                                                                            TAGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATT   <  SRR4302537.3071290/94‑1 (MQ=60)
                                                                                             AGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTG  >  SRR4302537.1344198/1‑94 (MQ=60)
                                                                                             AGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTG  <  SRR4302537.5639004/94‑1 (MQ=60)
                                                                                             AGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAAACACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTG  <  SRR4302537.3445647/94‑1 (MQ=60)
                                                                                             |                                                                                             
TATGAAAGTGAAGGTAAATATACCGTTAGAAATTTGGTTAAAAATAAAGCTATAGCATTGGAATTAGCTGAGATATATGTTAAGAATCGTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTG  >  NC_000913/3621156‑3621342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑