Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,737,956 2 bp→CG coding (623‑624/1254 nt) avtA → alanine‑alpha‑ketoisovalerate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,737,9560GC99.6% 940.2 / NA 274G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (129/142);  minor base T (1/0);  total (131/143)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,737,9570CG99.3% 959.9 / ‑4.7 274G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (128/144);  total (130/144)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043
                                                                                       ||                                                                                       
aaaCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATc                                                                                    <  1:4711100/93‑1 (MQ=255)
aaaCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATc                                                                                    >  1:6362506/1‑93 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAacac                                                                               >  2:1165986/1‑93 (MQ=255)
      ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACg                                                                              >  2:4597632/1‑93 (MQ=255)
      ggATGATTTGCGACTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACg                                                                              <  1:3060167/93‑1 (MQ=255)
        atgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGc                                                                            <  1:3524263/93‑1 (MQ=255)
        atgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGc                                                                            <  2:6404274/93‑1 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCa                                                                           >  2:3465860/1‑93 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAt                                                                          >  2:456833/1‑93 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAt                                                                          >  2:384221/1‑93 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTc                                                                        >  2:446664/1‑93 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTc                                                                        <  1:551858/93‑1 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTc                                                                        <  2:3669934/93‑1 (MQ=255)
                 gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTg                                                                   <  2:2346153/93‑1 (MQ=255)
                 gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTg                                                                   >  2:1431639/1‑93 (MQ=255)
                 gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTg                                                                   >  1:1895000/1‑93 (MQ=255)
                  tctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTgg                                                                  >  1:36156/1‑93 (MQ=255)
                  tctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTgg                                                                  <  2:2115376/93‑1 (MQ=255)
                  tctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTgg                                                                  <  1:4914130/93‑1 (MQ=255)
                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGt                                                                 <  1:665159/93‑1 (MQ=255)
                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGt                                                                 <  2:5637278/93‑1 (MQ=255)
                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGt                                                                 >  2:4401440/1‑93 (MQ=255)
                    tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGtg                                                                >  1:1760249/1‑93 (MQ=255)
                     cccGGCCGACGAATCCAACAGGCAATGTGATTACTGAGGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGtga                                                               <  2:1835778/93‑1 (MQ=255)
                     cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGtga                                                               <  2:1475517/93‑1 (MQ=255)
                     cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGtga                                                               <  1:4432194/93‑1 (MQ=255)
                      ccGGCCTACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGtgat                                                              <  1:5654985/93‑1 (MQ=255)
                       cgcccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGtgatt                                                             <  1:5142004/90‑1 (MQ=255)
                        ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGtgattg                                                            >  2:4875387/1‑93 (MQ=255)
                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  2:3610629/1‑93 (MQ=255)
                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  2:4301812/1‑93 (MQ=255)
                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  1:2757405/1‑93 (MQ=255)
                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  2:4073445/1‑93 (MQ=255)
                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  1:2420738/1‑93 (MQ=255)
                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  1:2597269/1‑93 (MQ=255)
                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  1:5757016/1‑93 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  2:767674/93‑1 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  1:393694/93‑1 (MQ=255)
                              cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  2:1938173/1‑93 (MQ=255)
                              cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  1:28268/1‑93 (MQ=255)
                              cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  1:5108409/1‑93 (MQ=255)
                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     >  1:5913618/1‑93 (MQ=255)
                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     >  1:1310727/1‑93 (MQ=255)
                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     <  1:1201284/93‑1 (MQ=255)
                                aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCt                                                    >  2:1099616/1‑93 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  1:2067458/93‑1 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  2:948684/93‑1 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  2:261117/93‑1 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               >  2:3806449/1‑93 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              >  2:3616845/1‑93 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  1:3262509/93‑1 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  1:6120074/93‑1 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              >  1:4227386/1‑93 (MQ=255)
                                         ggCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc                                           <  1:2220889/93‑1 (MQ=255)
                                         ggCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc                                           >  2:1559167/1‑93 (MQ=255)
                                          gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc                                          >  2:5083635/1‑93 (MQ=255)
                                           cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTccc                                         >  2:29154/1‑93 (MQ=255)
                                           cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTccc                                         <  2:4128575/93‑1 (MQ=255)
                                              tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  2:1404081/1‑93 (MQ=255)
                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    <  1:2875258/93‑1 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              >  1:4562427/1‑93 (MQ=255)
                                                       tGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTat                             >  1:1005053/1‑93 (MQ=255)
                                                       tGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTat                             >  1:5308382/1‑93 (MQ=255)
                                                          cGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat                          >  1:1712130/1‑93 (MQ=255)
                                                          cGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat                          >  2:5047563/1‑93 (MQ=255)
                                                          cGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat                          <  2:2213757/93‑1 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        >  2:6443354/1‑93 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        <  1:5556336/93‑1 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        >  2:3419549/1‑93 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGCCGTCCCGTTCCCAGGTATCATCt                        >  2:1007195/1‑93 (MQ=255)
                                                            aaGAGTTGCTAAAGCTTGACGCG‑TGGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAGGGCGTCCCGTTCCCGTGTATCATCt                        >  2:3307140/1‑93 (MQ=255)
                                                               agTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCCTCa                     <  1:626068/93‑1 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    >  1:977030/1‑93 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  2:6178521/93‑1 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  1:5885258/93‑1 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    >  2:3218620/1‑93 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  1:4086045/93‑1 (MQ=255)
                                                                 ttGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGt                   >  2:2741753/1‑93 (MQ=255)
                                                                   gCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 <  1:5983779/93‑1 (MQ=255)
                                                                    cTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                <  1:3304345/93‑1 (MQ=255)
                                                                     tGAAGCTTTACGCGCTGGCC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               >  2:6281215/1‑93 (MQ=255)
                                                                     tGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGCAg               >  2:28210/1‑93 (MQ=255)
                                                                     tGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  2:5098937/93‑1 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  1:3957467/93‑1 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  2:2542929/93‑1 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  1:756671/1‑93 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  1:820565/1‑93 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  2:6027309/1‑93 (MQ=255)
                                                                       aaGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             <  2:5877445/93‑1 (MQ=255)
                                                                        aGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgg            >  2:5371457/1‑92 (MQ=255)
                                                                        aGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgc            <  2:3056876/93‑1 (MQ=255)
                                                                        aGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgc            >  2:1980531/1‑93 (MQ=255)
                                                                           ttGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         >  2:915598/1‑93 (MQ=255)
                                                                                gcgcTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    <  1:3813649/93‑1 (MQ=255)
                                                                                gcgcTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    <  1:6184535/93‑1 (MQ=255)
                                                                                 cgcTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCTGTGTAGCGCGCCCGCTAt   >  2:5496379/1‑93 (MQ=255)
                                                                                 cgcTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   >  1:5242021/1‑93 (MQ=255)
                                                                                 cgcTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   <  2:5887812/93‑1 (MQ=255)
                                                                                 cgcTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   >  2:4818939/1‑93 (MQ=255)
                                                                                  gcTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATg  <  1:1109622/93‑1 (MQ=255)
                                                                                       ||                                                                                       
AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737868‑3738047
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GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATC                                                                                        >  SRR4302537.420041/1‑94 (MQ=60)
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATC                                                                                        >  SRR4302537.6405914/1‑94 (MQ=60)
 AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCA                                                                                       <  SRR4302537.4699568/94‑1 (MQ=60)
 AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCA                                                                                       <  SRR4302537.4743691/94‑1 (MQ=60)
   ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAC                                                                                     >  SRR4302537.5306012/1‑94 (MQ=60)
    CCGGGATGATTTGCGTCTGCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    <  SRR4302537.1731205/94‑1 (MQ=60)
     CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   >  SRR4302537.5706365/1‑94 (MQ=60)
      GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302537.6190237/94‑1 (MQ=60)
      GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302537.681589/94‑1 (MQ=60)
      GGGATGATTTGCGTCTACCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302537.819909/94‑1 (MQ=60)
       GGATGATTTGCGACTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGG                                                                                 <  SRR4302537.3081489/94‑1 (MQ=60)
        GATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGC                                                                                <  SRR4302537.5152985/94‑1 (MQ=60)
         ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               <  SRR4302537.2006306/94‑1 (MQ=60)
         ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               >  SRR4302537.3440327/1‑94 (MQ=60)
         ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               <  SRR4302537.3549195/94‑1 (MQ=60)
           GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             <  SRR4302537.792269/94‑1 (MQ=60)
            ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            >  SRR4302537.5929303/1‑94 (MQ=60)
             TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302537.3059715/94‑1 (MQ=60)
             TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302537.555655/94‑1 (MQ=60)
             TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302537.755822/94‑1 (MQ=60)
             TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302537.2412185/94‑1 (MQ=60)
               TGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGC                                                                         >  SRR4302537.4161802/1‑94 (MQ=60)
                GCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCT                                                                        >  SRR4302537.6107685/1‑94 (MQ=60)
                 CGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTG                                                                       >  SRR4302537.1908167/1‑94 (MQ=60)
                  GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGG                                                                      <  SRR4302537.3366193/94‑1 (MQ=60)
                  GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGG                                                                      >  SRR4302537.4040054/1‑94 (MQ=60)
                  GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGG                                                                      >  SRR4302537.36433/1‑94 (MQ=60)
                   TCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGT                                                                     <  SRR4302537.4948044/94‑1 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    <  SRR4302537.669691/94‑1 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    >  SRR4302537.2445344/1‑94 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    <  SRR4302537.151804/94‑1 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    <  SRR4302537.3634784/94‑1 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    >  SRR4302537.5234587/1‑94 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    >  SRR4302537.1772520/1‑94 (MQ=60)
                      CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  <  SRR4302537.4462799/94‑1 (MQ=60)
                      CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  >  SRR4302537.3076150/1‑94 (MQ=60)
                      CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTAGTGAT                                                                  >  SRR4302537.5640107/1‑94 (MQ=60)
                       CCGGCCTACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATT                                                                 <  SRR4302537.5693682/94‑1 (MQ=60)
                        CGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTG                                                                <  SRR4302537.2225982/94‑1 (MQ=60)
                        CGCCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTG                                                                <  SRR4302537.5177435/94‑1 (MQ=60)
                         GGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGA                                                               <  SRR4302537.6495199/94‑1 (MQ=60)
                          GCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGAG‑AATCAACACGGCATTCCGCTGGTGATTGAT                                                              <  SRR4302537.5897183/94‑1 (MQ=60)
                           CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302537.2776653/1‑94 (MQ=60)
                            CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302537.1871291/1‑94 (MQ=60)
                            CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302537.2615436/1‑94 (MQ=60)
                            CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302537.5796449/1‑94 (MQ=60)
                            CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302537.2437796/1‑94 (MQ=60)
                             GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           >  SRR4302537.5444083/1‑94 (MQ=60)
                             GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           >  SRR4302537.194756/1‑94 (MQ=60)
                             GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302537.3133956/94‑1 (MQ=60)
                              ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          <  SRR4302537.6370156/94‑1 (MQ=60)
                              ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          >  SRR4302537.5143611/1‑94 (MQ=60)
                              ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          >  SRR4302537.28493/1‑94 (MQ=60)
                              ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          <  SRR4302537.396425/94‑1 (MQ=60)
                               CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                         >  SRR4302537.5954206/1‑94 (MQ=60)
                               CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                         >  SRR4302537.1319851/1‑94 (MQ=60)
                                GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                        <  SRR4302537.1209636/94‑1 (MQ=60)
                                   TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302537.2081982/94‑1 (MQ=60)
                                   TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302537.1121143/94‑1 (MQ=60)
                                   TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302537.3795697/94‑1 (MQ=60)
                                   TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302537.2786729/94‑1 (MQ=60)
                                    CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302537.963091/94‑1 (MQ=60)
                                    CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    >  SRR4302537.2795213/1‑94 (MQ=60)
                                    CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302537.2168283/94‑1 (MQ=60)
                                     CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGTG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302537.6163406/1‑94 (MQ=60)
                                      AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  >  SRR4302537.4256821/1‑94 (MQ=60)
                                       ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                                 <  SRR4302537.3285521/94‑1 (MQ=60)
                                       ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                                 <  SRR4302537.6162141/94‑1 (MQ=60)
                                        CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                                >  SRR4302537.2244623/1‑94 (MQ=60)
                                        CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                                <  SRR4302537.4574023/94‑1 (MQ=60)
                                          GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              <  SRR4302537.2236539/94‑1 (MQ=60)
                                          GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              >  SRR4302537.105561/1‑94 (MQ=60)
                                           GCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCC                                             <  SRR4302537.4062529/94‑1 (MQ=60)
                                            CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            <  SRR4302537.6054659/94‑1 (MQ=60)
                                            CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            >  SRR4302537.6481825/1‑94 (MQ=60)
                                              ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                          >  SRR4302537.6263042/1‑94 (MQ=60)
                                              ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                          >  SRR4302537.99726/1‑94 (MQ=60)
                                                 TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302537.2969321/94‑1 (MQ=60)
                                                 TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302537.2895437/94‑1 (MQ=60)
                                                 TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302537.2660058/1‑94 (MQ=60)
                                                 TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302537.1747544/1‑94 (MQ=60)
                                                 TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302537.5205723/94‑1 (MQ=60)
                                                  GATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCG                                      <  SRR4302537.2894595/94‑1 (MQ=60)
                                                  GATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCG                                      <  SRR4302537.1286398/94‑1 (MQ=60)
                                                    TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                    <  SRR4302537.328656/94‑1 (MQ=60)
                                                    TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                    >  SRR4302537.1248686/1‑94 (MQ=60)
                                                      ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                  >  SRR4302537.4594008/1‑94 (MQ=60)
                                                       CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302537.1011990/1‑94 (MQ=60)
                                                       CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302537.5642257/1‑94 (MQ=60)
                                                       CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302537.5344855/1‑94 (MQ=60)
                                                        TGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATC                                >  SRR4302537.4829292/1‑94 (MQ=60)
                                                        TGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATC                                >  SRR4302537.3906500/1‑94 (MQ=60)
                                                        TGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATC                                >  SRR4302537.1000089/1‑94 (MQ=60)
                                                         GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                               <  SRR4302537.6400145/94‑1 (MQ=60)
                                                          ACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCAT                              >  SRR4302537.1724087/1‑94 (MQ=60)
                                                          ACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCAT                              <  SRR4302537.5573880/94‑1 (MQ=60)
                                                          ACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCAT                              >  SRR4302537.5413575/1‑94 (MQ=60)
                                                             AAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                           <  SRR4302537.5594300/94‑1 (MQ=60)
                                                             AAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                           >  SRR4302537.3110914/1‑94 (MQ=60)
                                                                AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        <  SRR4302537.2846967/94‑1 (MQ=60)
                                                                AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        >  SRR4302537.983770/1‑94 (MQ=60)
                                                                AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        <  SRR4302537.1133392/94‑1 (MQ=60)
                                                                AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        <  SRR4302537.4746684/94‑1 (MQ=60)
                                                                AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCCTCAG                        <  SRR4302537.630334/94‑1 (MQ=60)
                                                                 GTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                       >  SRR4302537.3558822/1‑94 (MQ=60)
                                                                 GTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                       <  SRR4302537.5925640/94‑1 (MQ=60)
                                                                 GTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                       <  SRR4302537.4114576/94‑1 (MQ=60)
                                                                 GTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                       >  SRR4302537.4996080/1‑94 (MQ=60)
                                                                 GTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                       >  SRR4302537.4358224/1‑94 (MQ=60)
                                                                 GTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                       <  SRR4302537.5145403/94‑1 (MQ=60)
                                                                    GCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    <  SRR4302537.6024848/94‑1 (MQ=60)
                                                                     CTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   <  SRR4302537.3327707/94‑1 (MQ=60)
                                                                     CTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   >  SRR4302537.5999191/1‑94 (MQ=60)
                                                                      TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  >  SRR4302537.826197/1‑94 (MQ=60)
                                                                      TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  <  SRR4302537.3967269/94‑1 (MQ=60)
                                                                      TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  >  SRR4302537.761834/1‑94 (MQ=60)
                                                                       GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302537.3985157/94‑1 (MQ=60)
                                                                       GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302537.2441709/94‑1 (MQ=60)
                                                                       GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 >  SRR4302537.1426265/1‑94 (MQ=60)
                                                                         AGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               <  SRR4302537.503287/94‑1 (MQ=60)
                                                                          GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC              <  SRR4302537.5968797/94‑1 (MQ=60)
                                                                          GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC              >  SRR4302537.5938950/1‑94 (MQ=60)
                                                                           CTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCC             >  SRR4302537.5566717/1‑94 (MQ=60)
                                                                             TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302537.4550556/1‑94 (MQ=60)
                                                                             TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302537.3896404/1‑94 (MQ=60)
                                                                              GACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGC          <  SRR4302537.4417746/94‑1 (MQ=60)
                                                                                 GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       >  SRR4302537.5278096/1‑94 (MQ=60)
                                                                                 GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302537.6227137/94‑1 (MQ=60)
                                                                                 GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302537.3840393/94‑1 (MQ=60)
                                                                                 GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302537.5707721/94‑1 (MQ=60)
                                                                                  CGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG      <  SRR4302537.3785635/94‑1 (MQ=60)
                                                                                  CGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG      >  SRR4302537.3429912/1‑94 (MQ=60)
                                                                                   GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302537.1117291/94‑1 (MQ=60)
                                                                                    CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    >  SRR4302537.1478289/1‑94 (MQ=60)
                                                                                       GGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  <  SRR4302537.4228401/94‑1 (MQ=60)
                                                                                        |                                                                                            
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737868‑3738047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑