Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,779,283 T→C L148L (TTG→CTG)  yibK → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,779,2830TC95.2% 640.9 / 14.5 208L148L (TTG→CTG) yibKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base C (91/106);  total (97/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 217 total aligned reads displayed.

AAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACA  >  NC_000913/3779194‑3779367
                                                                                         |                                                                                    
aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTgg                                                                                   <  2:5538582/93‑1 (MQ=255)
 aaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTggg                                                                                  <  1:3659029/93‑1 (MQ=255)
   aaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGt                                                                                <  1:2005122/93‑1 (MQ=255)
      atCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATc                                                                             <  2:4303401/92‑1 (MQ=255)
       tCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATcc                                                                            <  1:354191/93‑1 (MQ=255)
       tCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATcc                                                                            >  1:951385/1‑93 (MQ=255)
         gCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCgg                                                                          >  2:2357811/1‑93 (MQ=255)
         ccATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCgg                                                                          <  2:5996246/92‑1 (MQ=255)
           aTTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGa                                                                        <  2:5598467/93‑1 (MQ=255)
            ttCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAg                                                                       >  2:5739830/1‑93 (MQ=255)
             tCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATTAAGGCTGGCGGCGGCTGGGGTACCCGGGAGc                                                                      >  2:2716723/1‑93 (MQ=255)
             tCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAgg                                                                      >  1:1000587/1‑92 (MQ=255)
               cGATGGTGCCGGACAGCCGTAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCgg                                                                    >  2:6306637/1‑93 (MQ=255)
               cGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCgg                                                                    >  1:797619/1‑93 (MQ=255)
                 aTGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGGTGGGGTATCCGGGAGCGGTa                                                                  >  1:4863441/1‑93 (MQ=255)
                 aTGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTa                                                                  >  2:3258145/1‑93 (MQ=255)
                 aTGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTa                                                                  <  2:5771744/93‑1 (MQ=255)
                 aTGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTa                                                                  >  1:2928026/1‑93 (MQ=255)
                 aTGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGCATCCGGGAGCggga                                                                  >  2:1375891/1‑91 (MQ=255)
                  tGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTAt                                                                 <  1:1498130/93‑1 (MQ=255)
                   ggTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTAtt                                                                <  2:5053477/93‑1 (MQ=255)
                   ggTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTAtt                                                                <  1:773209/93‑1 (MQ=255)
                     ggCCGGACAGCCGCAGCATGAATCTGTCGAACGCGGTGTCCGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTga                                                              <  1:4887511/92‑1 (MQ=255)
                      gCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTgag                                                             >  2:5779194/1‑93 (MQ=255)
                       ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTgaga                                                            <  2:5176828/93‑1 (MQ=255)
                       ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTgaga                                                            <  1:1926016/93‑1 (MQ=255)
                        cGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTgagag                                                           <  2:5351680/93‑1 (MQ=255)
                        cGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTgagag                                                           >  1:5312291/1‑93 (MQ=255)
                        cGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTgagag                                                           <  2:3641687/93‑1 (MQ=255)
                          gACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGAt                                                         <  2:4964071/93‑1 (MQ=255)
                          gACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGAt                                                         >  1:5777147/1‑93 (MQ=255)
                          gACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGAt                                                         <  2:1202461/93‑1 (MQ=255)
                           aCAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGAtt                                                        <  2:5045818/93‑1 (MQ=255)
                           aCAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGAtt                                                        <  1:1270677/93‑1 (MQ=255)
                            cAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTa                                                       <  2:6355710/93‑1 (MQ=255)
                             aGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAg                                                      <  1:6103042/93‑1 (MQ=255)
                              gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGGGAGGTATTGAGAGATTAGt                                                     >  1:6211239/1‑93 (MQ=255)
                              gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGt                                                     <  2:5197460/93‑1 (MQ=255)
                              gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGGAGTGAGAGGTTAGt                                                     >  1:3343589/1‑93 (MQ=255)
                               ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGtt                                                    <  1:2664785/93‑1 (MQ=255)
                               ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGAGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGtt                                                    <  1:6475743/93‑1 (MQ=255)
                                 gcagcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTAc                                                  <  2:289853/93‑1 (MQ=255)
                                 gcagcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTAc                                                  >  2:809865/1‑93 (MQ=255)
                                 gcagcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTAc                                                  >  1:1691366/1‑93 (MQ=255)
                                  cagcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACt                                                 <  2:4899549/93‑1 (MQ=255)
                                  cagcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACt                                                 <  2:1632868/93‑1 (MQ=255)
                                    gcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGt                                               <  2:4095113/93‑1 (MQ=255)
                                    gcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGt                                               <  1:4720142/93‑1 (MQ=255)
                                    gcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGt                                               >  1:3090051/1‑93 (MQ=255)
                                     caTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTa                                              <  1:2240657/93‑1 (MQ=255)
                                      aTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAg                                             >  2:508942/1‑93 (MQ=255)
                                      aTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTaa                                             <  2:960255/93‑2 (MQ=255)
                                       tGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAgg                                            <  2:4513942/93‑1 (MQ=255)
                                       tGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAgg                                            >  1:4051562/1‑93 (MQ=255)
                                        gAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGc                                           <  1:1773134/93‑1 (MQ=255)
                                        gAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGc                                           >  1:6138706/1‑93 (MQ=255)
                                         aaTCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGcc                                          <  2:6376599/93‑1 (MQ=255)
                                         aaTCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGcc                                          <  1:6359421/93‑1 (MQ=255)
                                         aaTCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGCATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGcg                                          >  1:3029397/1‑92 (MQ=255)
                                           tCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGAGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCgg                                        >  1:4505014/1‑93 (MQ=255)
                                            cTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGa                                       <  1:1888294/93‑1 (MQ=255)
                                            cTGTCCAATGCGGTGTCGGTAGTAGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGa                                       >  2:4536153/1‑93 (MQ=255)
                                             tGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGAt                                      <  2:1020117/93‑1 (MQ=255)
                                             tGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGAt                                      >  1:5927567/1‑93 (MQ=255)
                                              gTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATa                                     >  2:3166868/1‑93 (MQ=255)
                                              gTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATa                                     <  1:3421023/93‑1 (MQ=255)
                                                ccAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAga                                   <  2:486188/93‑1 (MQ=255)
                                                ccAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAga                                   <  2:3667233/93‑1 (MQ=255)
                                                 cAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAgat                                  >  1:4000110/1‑93 (MQ=255)
                                                  aaTGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAgatg                                 <  2:4127031/93‑1 (MQ=255)
                                                  aaTGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAgatg                                 >  2:5905023/1‑93 (MQ=255)
                                                  aaTGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAgatg                                 >  2:234307/1‑93 (MQ=255)
                                                  aaTGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAgatg                                 >  2:1739919/1‑93 (MQ=255)
                                                    tGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAgatgat                               <  1:6050588/93‑1 (MQ=255)
                                                     gCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGAtt                              <  2:3324435/93‑1 (MQ=255)
                                                       ggTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTAc                            <  1:5084659/93‑1 (MQ=255)
                                                       ggTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTAc                            <  1:5210058/93‑1 (MQ=255)
                                                         tgtCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACAt                          <  2:5431908/93‑1 (MQ=255)
                                                           tCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCg                        >  1:2363413/1‑93 (MQ=255)
                                                            cGGTAGTGGTGTATTAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCga                       >  1:2135939/1‑92 (MQ=255)
                                                             ggTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCa                      >  2:3812028/1‑93 (MQ=255)
                                                              gTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCAt                     >  2:2454567/1‑93 (MQ=255)
                                                              gTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGGTACTGTAGGCCGGATAGATGATTACATCGCAt                     >  1:1086249/1‑93 (MQ=255)
                                                               tAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATc                    <  1:2523943/93‑1 (MQ=255)
                                                               tAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATc                    <  2:6123467/93‑1 (MQ=255)
                                                                aGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATcc                   <  2:1851543/93‑1 (MQ=255)
                                                                 gtggtgTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCg                  <  1:2829929/93‑1 (MQ=255)
                                                                   ggtgTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGc                <  1:1964073/93‑1 (MQ=255)
                                                                    gtgtATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCa               <  2:5409156/93‑1 (MQ=255)
                                                                    gtgtATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCa               <  1:6010855/93‑1 (MQ=255)
                                                                       tATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGa            >  2:3684081/1‑93 (MQ=255)
                                                                       tATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGa            >  1:2453124/1‑93 (MQ=255)
                                                                       tATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGa            <  2:2164233/93‑1 (MQ=255)
                                                                          gAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca         <  2:1363719/93‑1 (MQ=255)
                                                                           aaGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcac        >  1:702353/1‑93 (MQ=255)
                                                                            aGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca       >  1:4210152/1‑93 (MQ=255)
                                                                             gCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATAACAg      <  1:4383695/93‑1 (MQ=255)
                                                                              ccTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTATGCCGGATAGATGATTACATCGCATCCGGCACGATCACAgg     >  2:3966933/1‑93 (MQ=255)
                                                                                tGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGAc   >  2:5653324/1‑93 (MQ=255)
                                                                                tGGCGGCGGCGGGGGTGTCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACAGCGCACCCGGCACGATCACAGGAc   <  1:3730576/93‑1 (MQ=255)
                                                                                         |                                                                                    
AAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACA  >  NC_000913/3779194‑3779367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCC  >  NC_000913/3779193‑3779376
                                                                                          |                                                                                             
CAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGG                                                                                            >  SRR4302537.4057007/1‑94 (MQ=60)
 AAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGG                                                                                           <  SRR4302537.3858342/94‑1 (MQ=60)
  AAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGG                                                                                          <  SRR4302537.3034061/94‑1 (MQ=60)
  AAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGG                                                                                          <  SRR4302537.3684783/94‑1 (MQ=60)
    AAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTA                                                                                        >  SRR4302537.2448268/1‑94 (MQ=60)
    AAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTA                                                                                        <  SRR4302537.2019061/94‑1 (MQ=60)
    AAATTCGCATTCCGATGGTGCCGGACAGCCCCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTA                                                                                        >  SRR4302537.490513/1‑94 (MQ=60)
       TTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCC                                                                                     >  SRR4302537.957962/1‑94 (MQ=60)
        TCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCG                                                                                    <  SRR4302537.356656/94‑1 (MQ=60)
         CGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGG                                                                                   >  SRR4302537.5918407/1‑94 (MQ=60)
          GCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGG                                                                                  >  SRR4302537.1861699/1‑94 (MQ=60)
           CATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGA                                                                                 >  SRR4302537.2293699/1‑94 (MQ=60)
             TTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGG                                                                               >  SRR4302537.1007494/1‑94 (MQ=60)
               CCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGG                                                                             >  SRR4302537.803093/1‑94 (MQ=60)
                CGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGT                                                                            <  SRR4302537.2918251/94‑1 (MQ=60)
                CGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGT                                                                            >  SRR4302537.3374093/1‑94 (MQ=60)
                 GATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGGTGGGGTATCCGGGAGCGGTA                                                                           >  SRR4302537.4896995/1‑94 (MQ=60)
                 GATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTA                                                                           >  SRR4302537.2948588/1‑94 (MQ=60)
                 GATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTA                                                                           <  SRR4302537.3752861/94‑1 (MQ=60)
                  ATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTAT                                                                          >  SRR4302537.1947732/1‑94 (MQ=60)
                  ATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTCGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTAT                                                                          >  SRR4302537.3697879/1‑94 (MQ=60)
                   TGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATT                                                                         <  SRR4302537.1508589/94‑1 (MQ=60)
                    GGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTG                                                                        <  SRR4302537.778507/94‑1 (MQ=60)
                     GTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGA                                                                       >  SRR4302537.4681368/1‑94 (MQ=60)
                      TGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAG                                                                      <  SRR4302537.6064521/94‑1 (MQ=60)
                      GGCCGGACAGCCGCAGCATGAATCTGTCGAACGCGGTGTCCGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAG                                                                      <  SRR4302537.4921259/94‑1 (MQ=60)
                        CCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAG                                                                    >  SRR4302537.5348777/1‑94 (MQ=60)
                        CCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAG                                                                    <  SRR4302537.1939381/94‑1 (MQ=60)
                          GGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGAT                                                                  >  SRR4302537.5816748/1‑94 (MQ=60)
                          GGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGAT                                                                  >  SRR4302537.694136/1‑94 (MQ=60)
                            ACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTA                                                                >  SRR4302537.2222229/1‑94 (MQ=60)
                            ACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTA                                                                <  SRR4302537.1279513/94‑1 (MQ=60)
                            ACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTA                                                                <  SRR4302537.2983338/94‑1 (MQ=60)
                            ACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTA                                                                >  SRR4302537.2693246/1‑94 (MQ=60)
                             CAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGGATTGAGAGATTAG                                                               >  SRR4302537.4870477/1‑94 (MQ=60)
                              AGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGGGAGGTATTGAGAGATTAGT                                                              >  SRR4302537.6254027/1‑94 (MQ=60)
                              AGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGT                                                              <  SRR4302537.4008284/94‑1 (MQ=60)
                              AGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGT                                                              <  SRR4302537.6144975/94‑1 (MQ=60)
                              AGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGGAGTGAGAGGTTAGT                                                              >  SRR4302537.3367258/1‑94 (MQ=60)
                                CCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTA                                                            <  SRR4302537.5779603/94‑1 (MQ=60)
                                CCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTA                                                            >  SRR4302537.4356740/1‑94 (MQ=60)
                                CCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTA                                                            <  SRR4302537.2683398/94‑1 (MQ=60)
                                CCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGAGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTA                                                            <  SRR4302537.6519712/94‑1 (MQ=60)
                                 CGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTAT                                                           >  SRR4302537.3340253/1‑94 (MQ=60)
                                 CGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTAC                                                           >  SRR4302537.1703206/1‑94 (MQ=60)
                                  GCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACT                                                          <  SRR4302537.784123/94‑1 (MQ=60)
                                   CAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTG                                                         <  SRR4302537.6190157/94‑1 (MQ=60)
                                   CAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTG                                                         <  SRR4302537.3525651/94‑1 (MQ=60)
                                    AGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGT                                                        >  SRR4302537.3111603/1‑94 (MQ=60)
                                    AGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGGATCCGGGAGCGGTATTGAGAGATTAGTTACTGT                                                        >  SRR4302537.914709/1‑94 (MQ=60)
                                     GCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTA                                                       <  SRR4302537.4752780/94‑1 (MQ=60)
                                      CATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAG                                                      <  SRR4302537.4476095/94‑1 (MQ=60)
                                      CATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAG                                                      <  SRR4302537.1984644/94‑1 (MQ=60)
                                      CATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAG                                                      <  SRR4302537.2256453/94‑1 (MQ=60)
                                       ATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGG                                                     >  SRR4302537.4079894/1‑94 (MQ=60)
                                        TGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGC                                                    >  SRR4302537.6180915/1‑94 (MQ=60)
                                         GAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCC                                                   <  SRR4302537.1785485/94‑1 (MQ=60)
                                         GAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGCATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCG                                                   >  SRR4302537.3050516/1‑94 (MQ=60)
                                          AATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCG                                                  <  SRR4302537.6402810/94‑1 (MQ=60)
                                           ATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGAGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGG                                                 >  SRR4302537.4536166/1‑94 (MQ=60)
                                             CTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGAT                                               >  SRR4302537.5968246/1‑94 (MQ=60)
                                             CTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGAT                                               <  SRR4302537.1901423/94‑1 (MQ=60)
                                              TGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATA                                              >  SRR4302537.3105162/1‑94 (MQ=60)
                                               GTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAG                                             <  SRR4302537.3445235/94‑1 (MQ=60)
                                                 CCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGAT                                           >  SRR4302537.2105807/1‑94 (MQ=60)
                                                 CCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGAT                                           >  SRR4302537.4028116/1‑94 (MQ=60)
                                                  CAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATG                                          <  SRR4302537.1921187/94‑1 (MQ=60)
                                                     TGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATT                                       <  SRR4302537.6092146/94‑1 (MQ=60)
                                                     TGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATT                                       <  SRR4302537.984593/94‑1 (MQ=60)
                                                      GCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTA                                      <  SRR4302537.5014362/94‑1 (MQ=60)
                                                       CGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTAC                                     <  SRR4302537.2498524/94‑1 (MQ=60)
                                                        GGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACA                                    <  SRR4302537.5119654/94‑1 (MQ=60)
                                                        GGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACA                                    <  SRR4302537.5245887/94‑1 (MQ=60)
                                                        GGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACA                                    <  SRR4302537.5312720/94‑1 (MQ=60)
                                                          TGTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATACGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATC                                  <  SRR4302537.5997559/94‑1 (MQ=60)
                                                           GTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCG                                 >  SRR4302537.2380078/1‑94 (MQ=60)
                                                           GTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCG                                 >  SRR4302537.6156861/1‑94 (MQ=60)
                                                           GTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCG                                 <  SRR4302537.2646047/94‑1 (MQ=60)
                                                           GTCGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGGATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCG                                 >  SRR4302537.3660362/1‑94 (MQ=60)
                                                            TCGGTAGTGGTGTATTAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGA                                >  SRR4302537.2150979/1‑94 (MQ=60)
                                                             CGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCA                               >  SRR4302537.4650393/1‑94 (MQ=60)
                                                             CGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCA                               >  SRR4302537.1219524/1‑94 (MQ=60)
                                                             CGGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCA                               >  SRR4302537.520681/1‑94 (MQ=60)
                                                              GGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATAGCAT                              >  SRR4302537.6123406/1‑94 (MQ=60)
                                                              GGTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGGTACTGTAGGCCGGATAGATGATTACATCGCAT                              >  SRR4302537.1093746/1‑94 (MQ=60)
                                                               GTAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATC                             >  SRR4302537.2256515/1‑94 (MQ=60)
                                                                TAGTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCC                            <  SRR4302537.2541663/94‑1 (MQ=60)
                                                                  GTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGG                          <  SRR4302537.102816/94‑1 (MQ=60)
                                                                  GTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGG                          <  SRR4302537.2849702/94‑1 (MQ=60)
                                                                  GTGGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGG                          <  SRR4302537.1743173/94‑1 (MQ=60)
                                                                    GGTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCA                        <  SRR4302537.1977729/94‑1 (MQ=60)
                                                                     GTGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAC                       <  SRR4302537.6052152/94‑1 (MQ=60)
                                                                      TGTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACG                      >  SRR4302537.1835208/1‑94 (MQ=60)
                                                                       GTATGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGA                     >  SRR4302537.2470383/1‑94 (MQ=60)
                                                                          TGAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCA                  >  SRR4302537.6185951/1‑94 (MQ=60)
                                                                           GAAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCAC                 >  SRR4302537.707162/1‑94 (MQ=60)
                                                                           GAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCAC                 <  SRR4302537.759840/94‑1 (MQ=60)
                                                                            AAGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACA                >  SRR4302537.4239520/1‑94 (MQ=60)
                                                                             AGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAG               >  SRR4302537.6443641/1‑94 (MQ=60)
                                                                             AGCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAG               <  SRR4302537.927957/94‑1 (MQ=60)
                                                                             AGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAG               >  SRR4302537.5728684/1‑94 (MQ=60)
                                                                              GCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGG              <  SRR4302537.13167/94‑1 (MQ=60)
                                                                              GCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGG              <  SRR4302537.1693952/94‑1 (MQ=60)
                                                                              GCCTGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATAACAGG              <  SRR4302537.4413945/94‑1 (MQ=60)
                                                                                 TGGCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACA           <  SRR4302537.6331186/94‑1 (MQ=60)
                                                                                 TGGCGGCGGCGGGGGTGTCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACAGCGCACCCGGCACGATCACAGGACA           <  SRR4302537.3756781/94‑1 (MQ=60)
                                                                                   GCGGCGGCTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATT         >  SRR4302537.804735/1‑94 (MQ=60)
                                                                                   GCGGC‑‑‑TGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGA      <  SRR4302537.5871921/94‑1 (MQ=60)
                                                                                          CTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCC  >  SRR4302537.4783095/1‑94 (MQ=60)
                                                                                          CTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCC  >  SRR4302537.5376166/1‑94 (MQ=60)
                                                                                          CTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCC  >  SRR4302537.3356844/1‑94 (MQ=60)
                                                                                          |                                                                                             
CAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCC  >  NC_000913/3779193‑3779376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑