Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,799,570 T→C L19L (CTA→CTG)  rfaJ ← UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,799,5700TC95.2% 795.6 / 21.9 251L19L (CTA→CTG) rfaJUDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (8/4);  new base C (112/127);  total (120/131)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 267 total aligned reads displayed.

AATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTC  >  NC_000913/3799480‑3799659
                                                                                          |                                                                                         
 aTTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATtagccagc                                                                                        >  1:700662/1‑93 (MQ=255)
 aTTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATtagccagc                                                                                        >  1:5518041/1‑93 (MQ=255)
  ttGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTagccagcc                                                                                       <  2:5900271/93‑1 (MQ=255)
        tgatgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAAt                                                                                 >  2:6115021/1‑93 (MQ=255)
         gatgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATc                                                                                <  1:2088107/93‑1 (MQ=255)
          atgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATcc                                                                               >  1:3867359/1‑93 (MQ=255)
           tgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATccc                                                                              <  1:565917/93‑1 (MQ=255)
           tgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATccc                                                                              >  2:3594122/1‑93 (MQ=255)
           tgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATccc                                                                              >  2:3776239/1‑93 (MQ=255)
           tgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATccc                                                                              >  1:1922290/1‑93 (MQ=255)
           tgGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATccc                                                                              <  2:5961482/93‑1 (MQ=255)
            ggAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCa                                                                             >  1:1321470/1‑93 (MQ=255)
               aaCACCTGCACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAATCATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGc                                                                          <  2:2542989/93‑1 (MQ=255)
                    cTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTaa                                                                     <  1:1034316/93‑1 (MQ=255)
                     tACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAc                                                                    <  2:2148981/93‑1 (MQ=255)
                     tACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAc                                                                    >  2:892457/1‑93 (MQ=255)
                     tACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAc                                                                    >  1:429114/1‑93 (MQ=255)
                      acacCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACt                                                                   <  1:6275452/93‑1 (MQ=255)
                      acacCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACt                                                                   <  1:5273435/93‑1 (MQ=255)
                       cacCATCAGGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACtt                                                                  >  2:1933045/1‑93 (MQ=255)
                        acCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACttt                                                                 <  1:6438930/93‑1 (MQ=255)
                           aTCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatc                                                              >  2:5195065/1‑93 (MQ=255)
                            tCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatct                                                             >  2:4827103/1‑93 (MQ=255)
                             cAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatcta                                                            <  2:3116315/93‑1 (MQ=255)
                              aaGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatctat                                                           <  1:1840908/93‑1 (MQ=255)
                              aaGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATCAGCCAGCCGAAAATCCCAGGCTTTAACTTtatctat                                                           <  1:5090424/93‑1 (MQ=255)
                               aGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatctatc                                                          <  1:2484331/93‑1 (MQ=255)
                               aGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatctatc                                                          >  2:403341/1‑93 (MQ=255)
                               aGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatctatc                                                          >  2:6229882/1‑93 (MQ=255)
                               aGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatctatc                                                          <  2:2634500/93‑1 (MQ=255)
                               aGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTtatctatc                                                          <  2:1978675/93‑1 (MQ=255)
                                gATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTatctatct                                                         >  2:1413813/1‑93 (MQ=255)
                                gATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTatctatct                                                         >  2:1424213/1‑93 (MQ=255)
                                gATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTatctatct                                                         <  1:5174155/93‑1 (MQ=255)
                                 aTAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTAtctc                                                        >  2:3820564/1‑93 (MQ=255)
                                  tAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATctct                                                       <  2:6342567/93‑1 (MQ=255)
                                   aaTTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTa                                                      <  2:1615852/93‑1 (MQ=255)
                                   aaTTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTa                                                      >  1:4360076/1‑93 (MQ=255)
                                    aTTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTAt                                                     <  1:1254317/93‑1 (MQ=255)
                                     ttAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATg                                                    <  1:973749/93‑1 (MQ=255)
                                      tAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATgg                                                   <  2:4441067/93‑1 (MQ=255)
                                        gCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCa                                                 >  1:2901496/1‑93 (MQ=255)
                                          aTCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAgg                                               >  1:4794159/1‑93 (MQ=255)
                                           tCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGa                                              >  1:2831343/1‑93 (MQ=255)
                                           tCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGa                                              >  1:1108842/1‑93 (MQ=255)
                                              acacCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAAt                                           >  1:4971666/1‑93 (MQ=255)
                                              acacCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAAt                                           >  2:1199321/1‑93 (MQ=255)
                                                acCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGa                                         >  2:5124195/1‑93 (MQ=255)
                                                acCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGa                                         <  2:2779034/93‑1 (MQ=255)
                                                 ccATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAg                                        >  1:5333739/1‑93 (MQ=255)
                                                 ccATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAg                                        >  1:2638556/1‑93 (MQ=255)
                                                   aTAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTc                                      <  2:4228974/93‑1 (MQ=255)
                                                   aTAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTc                                      >  1:998246/1‑93 (MQ=255)
                                                   aTAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTc                                      <  2:791992/93‑1 (MQ=255)
                                                   aTAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTc                                      >  1:5052868/1‑93 (MQ=255)
                                                    tAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTcc                                     <  1:2699277/93‑1 (MQ=255)
                                                     aGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCca                                    <  2:562854/93‑1 (MQ=255)
                                                      ggCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATTATTCcaa                                   >  1:6040863/1‑92 (MQ=255)
                                                        cAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACaa                                 <  2:5462821/93‑1 (MQ=255)
                                                         aaCATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAAt                                <  2:2374458/93‑1 (MQ=255)
                                                          aCATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATg                               >  1:3567532/1‑93 (MQ=255)
                                                           cATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGc                              >  2:3491570/1‑93 (MQ=255)
                                                           cATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGc                              >  1:2045499/1‑93 (MQ=255)
                                                           cATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGc                              <  1:1424896/93‑1 (MQ=255)
                                                             tttAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTa                            <  2:6298105/93‑1 (MQ=255)
                                                               tAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTAcc                          >  1:4002788/1‑93 (MQ=255)
                                                                aaaCATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTAccc                         <  1:1163375/93‑1 (MQ=255)
                                                                aaaCATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTAccc                         <  1:4486131/93‑1 (MQ=255)
                                                                  aCATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCtt                       <  1:1602746/93‑1 (MQ=255)
                                                                  aCATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCtt                       >  2:1385835/1‑93 (MQ=255)
                                                                   cATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTATCCTta                      >  2:6060286/1‑93 (MQ=255)
                                                                   cATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTta                      >  2:4975618/1‑93 (MQ=255)
                                                                   cATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTta                      <  1:6159892/93‑1 (MQ=255)
                                                                   cATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTta                      >  2:3243268/1‑93 (MQ=255)
                                                                     ttCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTtata                    <  1:3689740/93‑1 (MQ=255)
                                                                      tCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTatat                   <  2:1255784/93‑1 (MQ=255)
                                                                       cAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATc                  <  2:1509170/93‑1 (MQ=255)
                                                                        aGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCa                 >  2:4544499/1‑93 (MQ=255)
                                                                        aGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCa                 <  2:429370/93‑1 (MQ=255)
                                                                          aaGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCAtt               <  2:3324241/93‑1 (MQ=255)
                                                                           aGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTa              >  1:4844910/1‑93 (MQ=255)
                                                                           aGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTa              <  2:3066275/93‑1 (MQ=255)
                                                                            gTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTAc             >  2:2964612/1‑93 (MQ=255)
                                                                            gTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTAc             <  1:3333855/93‑1 (MQ=255)
                                                                             tATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACt            <  1:906921/93‑1 (MQ=255)
                                                                             tATTTATATAAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACt            <  1:6178724/93‑1 (MQ=255)
                                                                              aTTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACtt           >  1:344796/1‑93 (MQ=255)
                                                                                ttATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTta         <  1:5413626/93‑1 (MQ=255)
                                                                                ttATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTta         <  2:1664958/93‑1 (MQ=255)
                                                                                 tataTTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTtat        >  2:4215137/1‑93 (MQ=255)
                                                                                 tataTTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTtat        >  1:5760873/1‑93 (MQ=255)
                                                                                  atatTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTtata       <  1:5952181/93‑1 (MQ=255)
                                                                                  atatTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTtata       >  2:1320790/1‑93 (MQ=255)
                                                                                  atatTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTtata       >  1:4627104/1‑93 (MQ=255)
                                                                                    atTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGt     <  2:5349572/93‑1 (MQ=255)
                                                                                    atTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGt     >  1:5924079/1‑93 (MQ=255)
                                                                                    atTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGt     >  2:4659239/1‑93 (MQ=255)
                                                                                     ttAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGtt    <  2:1797783/93‑1 (MQ=255)
                                                                                     ttAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGtt    >  2:2801560/1‑93 (MQ=255)
                                                                                       agccagccGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTc  <  1:3017731/93‑1 (MQ=255)
                                                                                          |                                                                                         
AATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTC  >  NC_000913/3799480‑3799659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAG  >  NC_000913/3799477‑3799663
                                                                                             |                                                                                             
GACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCC                                                                                               <  SRR4302537.5164743/94‑1 (MQ=60)
GACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCC                                                                                               <  SRR4302537.6045466/94‑1 (MQ=60)
 ACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCA                                                                                              <  SRR4302537.1945237/94‑1 (MQ=60)
 ACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCA                                                                                              >  SRR4302537.4090588/1‑94 (MQ=60)
 ACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCA                                                                                              >  SRR4302537.228678/1‑94 (MQ=60)
 ACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCA                                                                                              <  SRR4302537.3773983/94‑1 (MQ=60)
  CAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAG                                                                                             >  SRR4302537.5425711/1‑94 (MQ=60)
   AATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGC                                                                                            <  SRR4302537.1786273/94‑1 (MQ=60)
   AATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGC                                                                                            >  SRR4302537.5555719/1‑94 (MQ=60)
   AATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGC                                                                                            >  SRR4302537.6517041/1‑94 (MQ=60)
   AATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGC                                                                                            >  SRR4302537.705456/1‑94 (MQ=60)
    ATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCC                                                                                           <  SRR4302537.1482090/94‑1 (MQ=60)
    ATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCC                                                                                           >  SRR4302537.4341508/1‑94 (MQ=60)
     TTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCG                                                                                          <  SRR4302537.5200541/94‑1 (MQ=60)
     TTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCG                                                                                          <  SRR4302537.2878329/94‑1 (MQ=60)
      TGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGA                                                                                         >  SRR4302537.587957/1‑94 (MQ=60)
      TGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGA                                                                                         <  SRR4302537.2562222/94‑1 (MQ=60)
        ATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAA                                                                                       >  SRR4302537.926388/1‑94 (MQ=60)
          GTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAAT                                                                                     >  SRR4302537.947833/1‑94 (MQ=60)
            GATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCC                                                                                   <  SRR4302537.6396119/94‑1 (MQ=60)
            GATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCC                                                                                   <  SRR4302537.2102835/94‑1 (MQ=60)
            GATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCC                                                                                   >  SRR4302537.3894427/1‑94 (MQ=60)
             ATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCC                                                                                  >  SRR4302537.1935639/1‑94 (MQ=60)
             ATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCC                                                                                  <  SRR4302537.4131385/94‑1 (MQ=60)
              TGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCA                                                                                 >  SRR4302537.1330680/1‑94 (MQ=60)
              TGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCA                                                                                 >  SRR4302537.3528630/1‑94 (MQ=60)
              TGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCA                                                                                 <  SRR4302537.569805/94‑1 (MQ=60)
               GGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAG                                                                                >  SRR4302537.4776186/1‑94 (MQ=60)
                GAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGG                                                                               <  SRR4302537.5099626/94‑1 (MQ=60)
                GAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGG                                                                               <  SRR4302537.2011123/94‑1 (MQ=60)
                GAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGG                                                                               <  SRR4302537.1173131/94‑1 (MQ=60)
                   ACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTT                                                                            <  SRR4302537.1391011/94‑1 (MQ=60)
                    CACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTT                                                                           >  SRR4302537.4699116/1‑94 (MQ=60)
                       CTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAC                                                                        <  SRR4302537.1041441/94‑1 (MQ=60)
                       CTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAC                                                                        >  SRR4302537.432104/1‑94 (MQ=60)
                       CTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAC                                                                        <  SRR4302537.4568277/94‑1 (MQ=60)
                       CTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAC                                                                        >  SRR4302537.4991939/1‑94 (MQ=60)
                       CTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAAC                                                                        >  SRR4302537.1075171/1‑94 (MQ=60)
                        TACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACT                                                                       <  SRR4302537.3390908/94‑1 (MQ=60)
                        TACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACT                                                                       <  SRR4302537.3349810/94‑1 (MQ=60)
                         ACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTT                                                                      <  SRR4302537.3900126/94‑1 (MQ=60)
                         ACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTT                                                                      <  SRR4302537.5309725/94‑1 (MQ=60)
                         ACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTT                                                                      <  SRR4302537.1976942/94‑1 (MQ=60)
                         ACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTT                                                                      <  SRR4302537.6318510/94‑1 (MQ=60)
                          CACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTT                                                                     >  SRR4302537.6252047/1‑94 (MQ=60)
                           ACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTA                                                                    <  SRR4302537.6482639/94‑1 (MQ=60)
                             CATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATC                                                                  >  SRR4302537.2585504/1‑94 (MQ=60)
                              ATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCT                                                                 <  SRR4302537.1203828/94‑1 (MQ=60)
                              ATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCT                                                                 <  SRR4302537.1921074/94‑1 (MQ=60)
                              ATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCT                                                                 >  SRR4302537.4294710/1‑94 (MQ=60)
                               TCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTA                                                                <  SRR4302537.5333981/94‑1 (MQ=60)
                                 AAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATC                                                              <  SRR4302537.828021/94‑1 (MQ=60)
                                 AAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATC                                                              <  SRR4302537.5125472/94‑1 (MQ=60)
                                 AAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATC                                                              <  SRR4302537.1853717/94‑1 (MQ=60)
                                 AAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATCAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATC                                                              <  SRR4302537.5125471/94‑1 (MQ=60)
                                  AGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCT                                                             <  SRR4302537.1145636/94‑1 (MQ=60)
                                  AGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCT                                                             <  SRR4302537.2501820/94‑1 (MQ=60)
                                  AGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCT                                                             <  SRR4302537.1320288/94‑1 (MQ=60)
                                   GATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTC                                                            <  SRR4302537.5209771/94‑1 (MQ=60)
                                     TAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTA                                                          >  SRR4302537.4390246/1‑94 (MQ=60)
                                       ATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATG                                                        <  SRR4302537.1263043/94‑1 (MQ=60)
                                        TTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGG                                                       <  SRR4302537.980463/94‑1 (MQ=60)
                                         TAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGC                                                      >  SRR4302537.370010/1‑94 (MQ=60)
                                          AGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCA                                                     >  SRR4302537.2921880/1‑94 (MQ=60)
                                            CATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGG                                                   >  SRR4302537.3770688/1‑94 (MQ=60)
                                            CATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGG                                                   >  SRR4302537.4827247/1‑94 (MQ=60)
                                            CATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGG                                                   >  SRR4302537.1535787/1‑94 (MQ=60)
                                             ATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGA                                                  >  SRR4302537.4655883/1‑94 (MQ=60)
                                             ATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGA                                                  >  SRR4302537.2851126/1‑94 (MQ=60)
                                             ATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGA                                                  >  SRR4302537.1116507/1‑94 (MQ=60)
                                                GACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCCGGCTTTAACTTTATCTATCTCTATGGCAGGAAAA                                               >  SRR4302537.5005936/1‑94 (MQ=60)
                                                GACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAAT                                               <  SRR4302537.3424707/94‑1 (MQ=60)
                                                GACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAAT                                               >  SRR4302537.5005935/1‑94 (MQ=60)
                                                  CACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGA                                             <  SRR4302537.992632/94‑1 (MQ=60)
                                                  CACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGA                                             <  SRR4302537.2137456/94‑1 (MQ=60)
                                                  CACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGA                                             <  SRR4302537.6072477/94‑1 (MQ=60)
                                                   ACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAG                                            >  SRR4302537.2657015/1‑94 (MQ=60)
                                                   ACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAG                                            >  SRR4302537.5370315/1‑94 (MQ=60)
                                                     CATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTC                                          >  SRR4302537.1005134/1‑94 (MQ=60)
                                                     CATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTC                                          >  SRR4302537.5087656/1‑94 (MQ=60)
                                                     CATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTC                                          >  SRR4302537.2228062/1‑94 (MQ=60)
                                                      ATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCC                                         <  SRR4302537.6325047/94‑1 (MQ=60)
                                                      ATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCC                                         >  SRR4302537.5798087/1‑94 (MQ=60)
                                                      ATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCC                                         >  SRR4302537.6165406/1‑94 (MQ=60)
                                                      ATAGGCAACATTTAAACATTCAGAAGAATTTATATTAGCCAGCCGAACATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATTAGTCC                                         >  SRR4302537.2963288/1‑94 (MQ=60)
                                                       TAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCA                                        <  SRR4302537.2718133/94‑1 (MQ=60)
                                                        AGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATtattccaa                                       >  SRR4302537.6082362/1‑86 (MQ=60)
                                                        AGGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCAC                                       >  SRR4302537.1157481/1‑94 (MQ=60)
                                                         GGCAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACA                                      >  SRR4302537.3860466/1‑94 (MQ=60)
                                                           CAACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAAT                                    <  SRR4302537.4375831/94‑1 (MQ=60)
                                                            AACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATG                                   >  SRR4302537.3592688/1‑94 (MQ=60)
                                                            AACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATG                                   >  SRR4302537.5296886/1‑94 (MQ=60)
                                                             ACATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGC                                  >  SRR4302537.2059833/1‑94 (MQ=60)
                                                              CATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCT                                 <  SRR4302537.1434818/94‑1 (MQ=60)
                                                              CATTTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCT                                 <  SRR4302537.5612924/94‑1 (MQ=60)
                                                                 TTAAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACC                              >  SRR4302537.4030808/1‑94 (MQ=60)
                                                                   AAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCT                            <  SRR4302537.4517163/94‑1 (MQ=60)
                                                                   AAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCT                            <  SRR4302537.1171462/94‑1 (MQ=60)
                                                                   AAACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCT                            >  SRR4302537.5414112/1‑94 (MQ=60)
                                                                     ACATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTA                          <  SRR4302537.1613949/94‑1 (MQ=60)
                                                                      CATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTAT                         <  SRR4302537.6176283/94‑1 (MQ=60)
                                                                      CATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTAT                         <  SRR4302537.6202286/94‑1 (MQ=60)
                                                                      CATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTAT                         >  SRR4302537.2906989/1‑94 (MQ=60)
                                                                      CATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTAT                         >  SRR4302537.274743/1‑94 (MQ=60)
                                                                       ATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATA                        >  SRR4302537.5495510/1‑94 (MQ=60)
                                                                       ATTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATA                        <  SRR4302537.778554/94‑1 (MQ=60)
                                                                        TTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATAT                       <  SRR4302537.4514112/94‑1 (MQ=60)
                                                                        TTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATAT                       >  SRR4302537.5973939/1‑94 (MQ=60)
                                                                        TTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATAT                       <  SRR4302537.3715696/94‑1 (MQ=60)
                                                                        TTCAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATAT                       <  SRR4302537.3063009/94‑1 (MQ=60)
                                                                          CAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCA                     <  SRR4302537.5476449/94‑1 (MQ=60)
                                                                          CAGAAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCA                     >  SRR4302537.6236889/1‑94 (MQ=60)
                                                                             AAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTA                  >  SRR4302537.4878345/1‑94 (MQ=60)
                                                                             AAGTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTA                  <  SRR4302537.2079967/94‑1 (MQ=60)
                                                                               GTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACT                <  SRR4302537.2714815/94‑1 (MQ=60)
                                                                               GTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACT                <  SRR4302537.4905096/94‑1 (MQ=60)
                                                                               GTATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACT                <  SRR4302537.3357444/94‑1 (MQ=60)
                                                                                TATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTT               >  SRR4302537.347193/1‑94 (MQ=60)
                                                                                TATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTT               <  SRR4302537.913188/94‑1 (MQ=60)
                                                                                TATTTATATAAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTT               <  SRR4302537.6221281/94‑1 (MQ=60)
                                                                                 ATTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTT              >  SRR4302537.5055912/1‑94 (MQ=60)
                                                                                  TTTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTA             <  SRR4302537.5229482/94‑1 (MQ=60)
                                                                                   TTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTAT            <  SRR4302537.5951933/94‑1 (MQ=60)
                                                                                   TTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTAT            >  SRR4302537.5800336/1‑94 (MQ=60)
                                                                                   TTATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTAT            <  SRR4302537.5450536/94‑1 (MQ=60)
                                                                                    TATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATA           >  SRR4302537.4659123/1‑94 (MQ=60)
                                                                                     ATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAG          <  SRR4302537.6220833/94‑1 (MQ=60)
                                                                                     ATATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAG          <  SRR4302537.5993044/94‑1 (MQ=60)
                                                                                      TATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGT         >  SRR4302537.5964731/1‑94 (MQ=60)
                                                                                       ATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTT        >  SRR4302537.601869/1‑94 (MQ=60)
                                                                                       ATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTT        >  SRR4302537.1920084/1‑94 (MQ=60)
                                                                                       ATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTT        <  SRR4302537.2833657/94‑1 (MQ=60)
                                                                                       ATTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTT        <  SRR4302537.1407580/94‑1 (MQ=60)
                                                                                        TTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTT       <  SRR4302537.3663288/94‑1 (MQ=60)
                                                                                        TTAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTT       <  SRR4302537.5708060/94‑1 (MQ=60)
                                                                                         TAGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTC      <  SRR4302537.4855985/94‑1 (MQ=60)
                                                                                          AGCCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCC     <  SRR4302537.3038767/94‑1 (MQ=60)
                                                                                            CCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGCAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCA   >  SRR4302537.1624673/1‑94 (MQ=60)
                                                                                            CCAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCA   <  SRR4302537.2829925/94‑1 (MQ=60)
                                                                                             CAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAG  <  SRR4302537.1323418/94‑1 (MQ=60)
                                                                                             CAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAG  <  SRR4302537.4163491/94‑1 (MQ=60)
                                                                                             CAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAG  <  SRR4302537.981790/94‑1 (MQ=60)
                                                                                             |                                                                                             
GACAATTGATGTGATGGAAACACCTACACCATCAAGATAATTAGCATCGACACCATAGGCAACATTTAAACATTCAGAAGTATTTATATTAGCTAGCCGAAAATCCCAGGCTTTAACTTTATCTATCTCTATGGCAGGAAATGAGTCCACAATGCTACCCTTATATCATTACTTTATAGTTTCCCAG  >  NC_000913/3799477‑3799663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑