Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,841,093	G→A	G134G (GGC→GGT)	yicO ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,841,093	0	G	A	94.4%	815.7 / 21.4	250	G134G (GGC→GGT)	yicO	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (8/6); new base A (121/115); total (129/121)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-02

Only 100 of 255 total aligned reads displayed.

GATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCAT  >  NC_000913/3841003‑3841182

  tcatcCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCa                                                                                       <  1:776060/93‑1 (MQ=255)

   catcCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCAt                                                                                      <  2:1446979/93‑1 (MQ=255)

    atcCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATc                                                                                     <  2:535380/93‑1 (MQ=255)

    atcCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATc                                                                                     >  2:2793442/1‑93 (MQ=255)

       cAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGcc                                                                                  >  2:3867011/1‑93 (MQ=255)

       cAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGcc                                                                                  >  2:2263468/1‑93 (MQ=255)

         gTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGcccc                                                                                <  1:330137/93‑1 (MQ=255)

          tACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGccccc                                                                               <  1:4748992/93‑1 (MQ=255)

           aCCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCcca                                                                              <  1:6033350/93‑1 (MQ=255)

            ccGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccac                                                                             >  2:4163257/1‑93 (MQ=255)

            ccGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccac                                                                             <  2:4734549/93‑1 (MQ=255)

              ggATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCccacga                                                                           <  2:5183341/93‑1 (MQ=255)

               gATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCccacgac                                                                          >  1:2492910/1‑93 (MQ=255)

                 tACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCccacgacca                                                                        <  2:3938829/93‑1 (MQ=255)

                 tACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCccacgacca                                                                        >  1:4491084/1‑93 (MQ=255)

                 tACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCCGTCTGCCAGGAGATACCCATCGCCCccacgacca                                                                        >  1:1501137/1‑93 (MQ=255)

                    gAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCccacgaccacga                                                                     >  1:2921635/1‑93 (MQ=255)

                       aCAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAgg                                                                  >  2:4949532/1‑93 (MQ=255)

                        cAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGc                                                                 >  2:4307417/1‑93 (MQ=255)

                         aGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCa                                                                >  1:5322392/1‑93 (MQ=255)

                         aGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCa                                                                >  1:3498184/1‑93 (MQ=255)

                          gCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCaa                                                               <  2:692269/93‑1 (MQ=255)

                            gTGAGCAAAAATAGTCCACCTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAaag                                                             <  2:5215828/93‑1 (MQ=255)

                             tGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAaaga                                                            <  1:1895736/93‑1 (MQ=255)

                             tGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAaaga                                                            <  2:2607536/93‑1 (MQ=255)

                                gCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAgg                                                         >  1:4007336/1‑93 (MQ=255)

                                gCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAgg                                                         <  2:1835611/93‑1 (MQ=255)

                                 cAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGc                                                        >  1:3241142/1‑93 (MQ=255)

                                 cAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGc                                                        <  2:5496521/93‑1 (MQ=255)

                                 cAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGc                                                        <  1:4785553/93‑1 (MQ=255)

                                   aaaaTAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGTAGATACGCATCGCTCCCACGACCACGTAGGCAAAGAAGGCGt                                                      >  2:1110360/1‑93 (MQ=38)

                                   aaaaTAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGt                                                      >  1:5960382/1‑93 (MQ=255)

                                   aaaaTAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGt                                                      >  1:5535197/1‑93 (MQ=255)

                                   aaaaTAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGt                                                      >  2:511692/1‑93 (MQ=255)

                                     aaTAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTc                                                    >  2:5929583/1‑93 (MQ=255)

                                      aTAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGCTACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCa                                                   <  1:3462791/93‑1 (MQ=255)

                                      aTAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTcg                                                   >  2:5329029/1‑92 (MQ=255)

                                        aGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGc                                                 <  2:5815507/93‑1 (MQ=255)

                                         gTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGcc                                                >  2:4681529/1‑93 (MQ=255)

                                         gTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGcc                                                <  1:4128233/93‑1 (MQ=255)

                                          tCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGccc                                               >  2:4428220/1‑93 (MQ=255)

                                           ccAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGcccc                                              >  1:4056276/1‑93 (MQ=255)

                                           ccAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGcccc                                              >  1:718259/1‑93 (MQ=255)

                                             aaCTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCAt                                            >  2:6009370/1‑93 (MQ=255)

                                              aCTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCAtt                                           <  1:171013/93‑1 (MQ=255)

                                              aCTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCAtt                                           >  2:1373548/1‑93 (MQ=255)

                                                tGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGc                                         <  1:4958469/93‑1 (MQ=255)

                                                tGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGc                                         >  1:696056/1‑93 (MQ=255)

                                                     cccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAg                                    >  2:6197685/1‑93 (MQ=255)

                                                      ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGc                                   <  2:3175401/93‑1 (MQ=255)

                                                      ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGc                                   <  2:311202/93‑1 (MQ=255)

                                                      ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGc                                   >  2:1250515/1‑93 (MQ=255)

                                                      ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGc                                   <  2:2018299/93‑1 (MQ=255)

                                                        aGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCACAGAAGGCGTTCAGCCCCATTGCCGGAGCCa                                 >  1:2003036/1‑93 (MQ=255)

                                                        aGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCa                                 >  2:1293504/1‑93 (MQ=255)

                                                        aGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCa                                 >  1:6003180/1‑93 (MQ=255)

                                                          aataatGCCCCCATCCCGGTCTTCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAgc                               <  1:1793017/93‑1 (MQ=255)

                                                           atatTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAgcg                              >  1:3088962/1‑93 (MQ=255)

                                                            tatTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAgcgc                             <  1:301592/93‑1 (MQ=255)

                                                            tatTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAgcgc                             >  2:1684721/1‑93 (MQ=255)

                                                              ttGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCa                           <  1:4976120/93‑1 (MQ=255)

                                                              ttGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCa                           >  1:972187/1‑93 (MQ=255)

                                                               tGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCAc                          <  2:1552525/93‑1 (MQ=255)

                                                                gcgcCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACg                         >  1:3953623/1‑93 (MQ=255)

                                                                 cgcCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACgg                        <  1:209368/93‑1 (MQ=255)

                                                                  gcCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACggg                       >  1:1037936/1‑93 (MQ=255)

                                                                    ccATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTa                     <  1:4301539/93‑1 (MQ=255)

                                                                     cATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTaa                    >  2:2928112/1‑93 (MQ=255)

                                                                      aTCCCGGTCTGCCAGGAGATACCCATCGCCCCAACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAg                   >  2:4850679/1‑93 (MQ=255)

                                                                       tCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTACGt                  >  2:1606168/1‑93 (MQ=255)

                                                                       tCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGt                  <  1:2620077/93‑1 (MQ=255)

                                                                       tCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGt                  <  1:6445476/93‑1 (MQ=255)

                                                                         ccGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTa                >  1:2710172/1‑93 (MQ=255)

                                                                         ccGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTa                >  2:2483507/1‑93 (MQ=255)

                                                                         ccGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTa                <  2:2046588/93‑1 (MQ=255)

                                                                          cGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAg               <  1:951993/93‑1 (MQ=255)

                                                                          cGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAg               >  2:3526707/1‑93 (MQ=255)

                                                                           ggTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGc              >  1:1825781/1‑93 (MQ=255)

                                                                           ggTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGc              >  2:1935522/1‑93 (MQ=255)

                                                                           ggTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGc              <  1:5763635/93‑1 (MQ=255)

                                                                            gTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCa             >  1:841317/1‑93 (MQ=255)

                                                                             tCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCaa            <  1:4268427/93‑1 (MQ=255)

                                                                              cTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCaaa           <  1:4504395/93‑1 (MQ=255)

                                                                               tGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAat          <  1:1053316/93‑1 (MQ=255)

                                                                               tGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAat          >  1:1324159/1‑93 (MQ=255)

                                                                               tGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAat          <  2:5781734/93‑1 (MQ=255)

                                                                               tGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAat          <  1:2972138/93‑1 (MQ=255)

                                                                                gCCAGGAGATACCCATCGCCCCCACGACCACGAATGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAata         <  1:4709869/93‑1 (MQ=255)

                                                                                gCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAata         <  1:6280233/93‑1 (MQ=255)

                                                                                gCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAata         <  2:3424362/93‑1 (MQ=255)

                                                                                 ccAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAatat        >  2:5437818/1‑93 (MQ=255)

                                                                                 ccAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAatat        <  1:2376273/93‑1 (MQ=255)

                                                                                  cAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGGGCCACGGGCAGGTTAGCAACTATc       >  1:5236108/1‑93 (MQ=255)

                                                                                  cAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATc       <  2:6338722/93‑1 (MQ=255)

                                                                                   aGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATcc      <  2:4547868/93‑1 (MQ=255)

                                                                                    ggAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATccc     >  2:3804153/1‑93 (MQ=255)

                                                                                    ggAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATccc     <  1:2866609/93‑1 (MQ=255)

                                                                                    ggAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATccc     >  2:2238440/1‑93 (MQ=255)

                                                                                     gagaTACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATcccc    <  1:1389142/93‑1 (MQ=255)

                                                                                     gagaTACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATcccc    <  2:4126663/93‑1 (MQ=255)

GATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCAT  >  NC_000913/3841003‑3841182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCG  >  NC_000913/3841001‑3841184

GAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATAC                                                                                            <  SRR4302537.4446158/94‑1 (MQ=60)

    TCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCAT                                                                                        <  SRR4302537.4901041/94‑1 (MQ=60)

    TCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCAT                                                                                        <  SRR4302537.781383/94‑1 (MQ=60)

     CATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATC                                                                                       <  SRR4302537.5006214/94‑1 (MQ=60)

     CATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATC                                                                                       <  SRR4302537.364479/94‑1 (MQ=60)

     CATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATC                                                                                       >  SRR4302537.5965441/1‑94 (MQ=60)

      ATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCG                                                                                      <  SRR4302537.2992445/94‑1 (MQ=60)

         CAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCC                                                                                   >  SRR4302537.4535202/1‑94 (MQ=60)

           GTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCC                                                                                 <  SRR4302537.332428/94‑1 (MQ=60)

            TACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCTCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCA                                                                                <  SRR4302537.318573/94‑1 (MQ=60)

            TACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCA                                                                                <  SRR4302537.4781797/94‑1 (MQ=60)

             ACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCAC                                                                               <  SRR4302537.1933810/94‑1 (MQ=60)

             ACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCAC                                                                               <  SRR4302537.6074806/94‑1 (MQ=60)

             ACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCAC                                                                               <  SRR4302537.961158/94‑1 (MQ=60)

              CCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACG                                                                              >  SRR4302537.2944512/1‑94 (MQ=60)

                GGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGAC                                                                            >  SRR4302537.2510463/1‑94 (MQ=60)

                GGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGAC                                                                            >  SRR4302537.4069721/1‑94 (MQ=60)

                GGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGAC                                                                            <  SRR4302537.4742915/94‑1 (MQ=60)

                 GATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACC                                                                           <  SRR4302537.4787077/94‑1 (MQ=60)

                  ATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCA                                                                          >  SRR4302537.4522152/1‑94 (MQ=60)

                  ATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCCGTCTGCCAGGAGATACCCATCGCCCCCACGACCA                                                                          >  SRR4302537.1511616/1‑94 (MQ=60)

                    ACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACG                                                                        <  SRR4302537.1048508/94‑1 (MQ=60)

                    ACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACG                                                                        <  SRR4302537.1665951/94‑1 (MQ=60)

                     CGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGA                                                                       >  SRR4302537.2942163/1‑94 (MQ=60)

                      GAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAA                                                                      >  SRR4302537.1341598/1‑94 (MQ=60)

                       AAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAG                                                                     <  SRR4302537.1891875/94‑1 (MQ=60)

                       AAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAG                                                                     <  SRR4302537.339663/94‑1 (MQ=60)

                         ACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGC                                                                   >  SRR4302537.6030956/1‑94 (MQ=60)

                          CAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCA                                                                  >  SRR4302537.3522936/1‑94 (MQ=60)

                          CAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCA                                                                  >  SRR4302537.5358919/1‑94 (MQ=60)

                           AGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCACCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAA                                                                 >  SRR4302537.476208/1‑94 (MQ=60)

                            GCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAA                                                                <  SRR4302537.1832160/94‑1 (MQ=60)

                              GTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGA                                                              >  SRR4302537.3796552/1‑94 (MQ=60)

                               TGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAA                                                             <  SRR4302537.1908908/94‑1 (MQ=60)

                               TGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAA                                                             >  SRR4302537.4053294/1‑94 (MQ=60)

                                 AGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGG                                                           <  SRR4302537.714730/94‑1 (MQ=60)

                                 AGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGG                                                           >  SRR4302537.4035385/1‑94 (MQ=60)

                                  GCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGC                                                          >  SRR4302537.3263985/1‑94 (MQ=60)

                                   CAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCG                                                         <  SRR4302537.4818586/94‑1 (MQ=60)

                                    AAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGT                                                        >  SRR4302537.6001292/1‑94 (MQ=60)

                                    AAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGT                                                        >  SRR4302537.5572978/1‑94 (MQ=60)

                                        acnancnanCTGCNCCCCAGAATATTGCGCNCAACCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAAAAGGCGTTCAG                                                    <  SRR4302537.501740/85‑1 (MQ=60)

                                        ATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGCTACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAG                                                    <  SRR4302537.3487273/94‑1 (MQ=60)

                                        ATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAG                                                    >  SRR4302537.5400067/1‑94 (MQ=60)

                                           GTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCC                                                 <  SRR4302537.4157046/94‑1 (MQ=60)

                                            TCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCC                                                >  SRR4302537.723167/1‑94 (MQ=60)

                                            TCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCC                                                >  SRR4302537.4084621/1‑94 (MQ=60)

                                             CCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCA                                               >  SRR4302537.232656/1‑94 (MQ=60)

                                              CAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCAT                                              >  SRR4302537.96677/1‑94 (MQ=60)

                                               AACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATT                                             >  SRR4302537.5155035/1‑94 (MQ=60)

                                               AACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATT                                             <  SRR4302537.328738/94‑1 (MQ=60)

                                               AACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATT                                             <  SRR4302537.2886892/94‑1 (MQ=60)

                                               AACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATT                                             <  SRR4302537.131249/94‑1 (MQ=60)

                                                ACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTG                                            <  SRR4302537.172232/94‑1 (MQ=60)

                                                ACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGACATACCCATCGCCCCCACGACCCCGAAGGCAAAGAAGGCGTTCAGCCCCATTG                                            <  SRR4302537.5327880/94‑1 (MQ=60)

                                                 CTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGC                                           >  SRR4302537.2672816/1‑94 (MQ=60)

                                                 CTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGC                                           >  SRR4302537.700827/1‑94 (MQ=60)

                                                  TGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCC                                          <  SRR4302537.4992650/94‑1 (MQ=60)

                                                   GCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCG                                         <  SRR4302537.3180562/94‑1 (MQ=60)

                                                    CGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGG                                        <  SRR4302537.3517087/94‑1 (MQ=60)

                                                     GCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGA                                       <  SRR4302537.3999675/94‑1 (MQ=60)

                                                     GCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGA                                       >  SRR4302537.1779129/1‑94 (MQ=60)

                                                      CCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAG                                      >  SRR4302537.2102649/1‑94 (MQ=60)

                                                      CCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAG                                      >  SRR4302537.854597/1‑94 (MQ=60)

                                                       CCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGC                                     >  SRR4302537.726303/1‑94 (MQ=60)

                                                       CCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGC                                     >  SRR4302537.6432724/1‑94 (MQ=60)

                                                       CCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGC                                     >  SRR4302537.1452836/1‑94 (MQ=60)

                                                       CCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGC                                     >  SRR4302537.745773/1‑94 (MQ=60)

                                                        CCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCC                                    >  SRR4302537.1908174/1‑94 (MQ=60)

                                                         CAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCA                                   >  SRR4302537.3412595/1‑94 (MQ=60)

                                                         CAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCACAGAAGGCGTTCAGCCCCATTGCCGGAGCCA                                   >  SRR4302537.2016966/1‑94 (MQ=60)

                                                         CAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCA                                   >  SRR4302537.6044421/1‑94 (MQ=60)

                                                           GAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGC                                 >  SRR4302537.5602438/1‑94 (MQ=60)

                                                           GAATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGC                                 >  SRR4302537.4500296/1‑94 (MQ=60)

                                                            AATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCG                                >  SRR4302537.3110508/1‑94 (MQ=60)

                                                            AATAATGCCCCCATCCCGGTCTTCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCG                                <  SRR4302537.1805531/94‑1 (MQ=60)

                                                             ATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGC                               >  SRR4302537.2796513/1‑94 (MQ=60)

                                                             ATATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGC                               <  SRR4302537.2448085/94‑1 (MQ=60)

                                                              TATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCC                              >  SRR4302537.6290327/1‑94 (MQ=60)

                                                              TATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCC                              <  SRR4302537.303692/94‑1 (MQ=60)

                                                               ATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCA                             <  SRR4302537.2066285/94‑1 (MQ=60)

                                                               ATTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCA                             >  SRR4302537.978890/1‑94 (MQ=60)

                                                               ATTGCCCCCATCCCGGGCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCA                             <  SRR4302537.2816419/94‑1 (MQ=60)

                                                                TTGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCAC                            <  SRR4302537.5010417/94‑1 (MQ=60)

                                                                 TGCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACG                           >  SRR4302537.3981277/1‑94 (MQ=60)

                                                                  GCGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGG                          >  SRR4302537.4165290/1‑94 (MQ=60)

                                                                   CGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCACAGACGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGG                         >  SRR4302537.818142/1‑94 (MQ=60)

                                                                   CGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGG                         <  SRR4302537.525368/94‑1 (MQ=60)

                                                                   CGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGG                         <  SRR4302537.210873/94‑1 (MQ=60)

                                                                   CGCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGG                         >  SRR4302537.1045081/1‑94 (MQ=60)

                                                                    GCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGT                        >  SRR4302537.2581438/1‑94 (MQ=60)

                                                                    GCCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGT                        <  SRR4302537.4509146/94‑1 (MQ=60)

                                                                     CCCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTA                       >  SRR4302537.567208/1‑94 (MQ=60)

                                                                      CCATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAA                      <  SRR4302537.4331433/94‑1 (MQ=60)

                                                                      CCATCACGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAA                      <  SRR4302537.6042774/94‑1 (MQ=60)

                                                                       CATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGGAAG                     >  SRR4302537.1506083/1‑94 (MQ=60)

                                                                       CATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAG                     >  SRR4302537.2999635/1‑94 (MQ=60)

                                                                       CATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAG                     <  SRR4302537.6203222/94‑1 (MQ=60)

                                                                       CATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAG                     >  SRR4302537.4684621/1‑94 (MQ=60)

                                                                        ATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGT                    >  SRR4302537.4532174/1‑94 (MQ=60)

                                                                        ATCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGT                    >  SRR4302537.493928/1‑94 (MQ=60)

                                                                         TCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTT                   <  SRR4302537.4163911/94‑1 (MQ=60)

                                                                         TCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTT                   <  SRR4302537.1215933/94‑1 (MQ=60)

                                                                         TCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTT                   <  SRR4302537.6489204/94‑1 (MQ=60)

                                                                         TCCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTT                   <  SRR4302537.2638394/94‑1 (MQ=60)

                                                                          CCCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTA                  >  SRR4302537.2729098/1‑94 (MQ=60)

                                                                           CCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAG                 >  SRR4302537.410336/1‑94 (MQ=60)

                                                                           CCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAG                 <  SRR4302537.681096/94‑1 (MQ=60)

                                                                           CCGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAG                 <  SRR4302537.2310613/94‑1 (MQ=60)

                                                                            CGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGC                >  SRR4302537.1838505/1‑94 (MQ=60)

                                                                            CGGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGC                <  SRR4302537.958571/94‑1 (MQ=60)

                                                                             GGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCA               >  SRR4302537.847080/1‑94 (MQ=60)

                                                                             GGTCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCA               <  SRR4302537.5803118/94‑1 (MQ=60)

                                                                               TCTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAA             <  SRR4302537.4298084/94‑1 (MQ=60)

                                                                                CTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAAT            <  SRR4302537.3088427/94‑1 (MQ=60)

                                                                                CTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAAT            >  SRR4302537.3046146/1‑94 (MQ=60)

                                                                                CTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAAT            <  SRR4302537.4535544/94‑1 (MQ=60)

                                                                                CTGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAAT            >  SRR4302537.1333393/1‑94 (MQ=60)

                                                                                 TGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATA           <  SRR4302537.2992963/94‑1 (MQ=60)

                                                                                 TGCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATA           <  SRR4302537.1060581/94‑1 (MQ=60)

                                                                                  GCCAGGAGATACCCATCGCCCCCACGACCACGAATGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATAT          <  SRR4302537.4742453/94‑1 (MQ=60)

                                                                                  GCCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATAT          <  SRR4302537.6323309/94‑1 (MQ=60)

                                                                                   CCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGGGCCACGGGCAGGTTAGCAACTATC         >  SRR4302537.5272133/1‑94 (MQ=60)

                                                                                   CCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATC         >  SRR4302537.1747298/1‑94 (MQ=60)

                                                                                   CCAGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATC         <  SRR4302537.2393016/94‑1 (MQ=60)

                                                                                     AGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCC       >  SRR4302537.699174/1‑94 (MQ=60)

                                                                                     AGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCC       >  SRR4302537.2589432/1‑94 (MQ=60)

                                                                                     AGGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCC       >  SRR4302537.4325733/1‑94 (MQ=60)

                                                                                      GGAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCC      <  SRR4302537.2886719/94‑1 (MQ=60)

                                                                                       GAGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCA     <  SRR4302537.1398827/94‑1 (MQ=60)

                                                                                        AGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCAT    <  SRR4302537.5432961/94‑1 (MQ=60)

                                                                                        AGATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCAT    <  SRR4302537.86515/94‑1 (MQ=60)

                                                                                         GATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATC   >  SRR4302537.12985/1‑94 (MQ=60)

                                                                                         GATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATC   <  SRR4302537.6045562/94‑1 (MQ=60)

                                                                                          ATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCG  >  SRR4302537.3730126/1‑94 (MQ=60)

                                                                                          ATACCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCG  <  SRR4302537.4472070/94‑1 (MQ=60)

GAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCG  >  NC_000913/3841001‑3841184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`