Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,869,844 T→C H466R (CAC→CGC)  dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,869,8440TC96.0% 711.3 / 9.9 225H466R (CAC→CGC) dgoA2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (6/3);  new base C (92/124);  total (98/127)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 233 total aligned reads displayed.

AATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGTGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCC  >  NC_000913/3869753‑3869933
                                                                                           |                                                                                         
aaTCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGTg                                                                                          >  2:3398076/1‑93 (MQ=255)
   cgttCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGga                                                                                       <  1:782723/90‑1 (MQ=255)
   cGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGga                                                                                       <  2:3127661/93‑1 (MQ=255)
    ggTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGgag                                                                                      >  1:1360505/1‑93 (MQ=255)
     gTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGgaga                                                                                     <  1:1115367/93‑1 (MQ=255)
       ccGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGTGGgagaga                                                                                   <  2:2763983/93‑1 (MQ=255)
       ccGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGgagaga                                                                                   >  1:6418180/1‑93 (MQ=255)
       ccGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGgagaga                                                                                   <  1:3288088/93‑1 (MQ=255)
        cGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGAt                                                                                  <  1:4857095/93‑1 (MQ=255)
        cGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGAt                                                                                  <  1:403896/93‑1 (MQ=255)
         gAGCGGACAGTGCGGCGCAAGGGTCCCGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATc                                                                                 <  2:678140/93‑1 (MQ=255)
          aGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATcc                                                                                <  2:5203972/93‑1 (MQ=255)
            cGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCgg                                                                              >  2:1358533/1‑93 (MQ=255)
             ggACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGt                                                                             <  1:3842129/93‑1 (MQ=255)
               aCAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTg                                                                           >  2:1551299/1‑93 (MQ=255)
                  gTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAg                                                                        >  2:3727567/1‑93 (MQ=255)
                    gcggcgCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGaa                                                                      <  1:366009/93‑1 (MQ=255)
                     cggcgCAAGGGTCCCGTCATAGGGTTTCGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAAt                                                                     <  2:3967129/93‑1 (MQ=255)
                     cggcgCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAAt                                                                     <  2:796963/93‑1 (MQ=255)
                      ggcgCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATc                                                                    <  1:1266809/93‑1 (MQ=255)
                      ggcgCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATc                                                                    <  1:3443426/93‑1 (MQ=255)
                      ggcgCAAGGGACACGTCATAGGGTTCTGCCATTCCGGCGATGTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATc                                                                    <  2:4129442/93‑1 (MQ=255)
                         gcAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGTGGGAGAGATCCGGTTGCAGAATCGaa                                                                 >  2:5079835/1‑93 (MQ=255)
                         gcAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGaa                                                                 >  2:2120605/1‑93 (MQ=255)
                           aaGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAAt                                                               >  1:4748172/1‑93 (MQ=255)
                            aGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATa                                                              >  1:2894358/1‑93 (MQ=255)
                             gggTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATac                                                             >  2:3645483/1‑93 (MQ=255)
                              ggTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATacc                                                            <  2:5287343/93‑1 (MQ=255)
                              ggTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATacc                                                            <  2:5753262/93‑1 (MQ=255)
                              ggTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATacc                                                            >  1:5527777/1‑93 (MQ=255)
                                  aCGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCt                                                        >  1:4577704/1‑93 (MQ=255)
                                  aCGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCt                                                        >  1:222187/1‑93 (MQ=255)
                                   cGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTg                                                       >  2:5713041/1‑93 (MQ=255)
                                     tCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGcc                                                     <  2:4779794/93‑1 (MQ=255)
                                      cATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCt                                                    >  2:3482521/1‑93 (MQ=255)
                                       aTAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTc                                                   >  1:318276/1‑93 (MQ=255)
                                        tAGGCTTCTGCCATTCCGGCGATTTTGTACCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTcc                                                  >  1:4007325/1‑93 (MQ=255)
                                         aGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCa                                                 <  2:2993054/93‑1 (MQ=255)
                                         aGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGATGCAGAATCGAAATACCACGTGCCTCCa                                                 >  2:1103705/1‑93 (MQ=255)
                                           gCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGc                                               <  1:3144335/93‑1 (MQ=255)
                                            cTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCa                                              <  2:3676264/93‑1 (MQ=255)
                                             ttCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAc                                             >  2:4954733/1‑93 (MQ=255)
                                               cTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAcgc                                           <  2:4368474/93‑1 (MQ=255)
                                               cTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAcgc                                           <  2:2385534/93‑1 (MQ=255)
                                               cTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAcgc                                           <  1:985267/93‑1 (MQ=255)
                                                tGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAcgcg                                          >  2:1761683/1‑93 (MQ=255)
                                                 gCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGt                                         >  1:5790124/1‑93 (MQ=255)
                                                 gCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGt                                         <  1:751360/93‑1 (MQ=255)
                                                  ccATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGtt                                        <  1:3266841/93‑1 (MQ=255)
                                                  ccATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGtt                                        <  2:4870638/93‑1 (MQ=255)
                                                   cATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGttt                                       >  2:5949024/1‑93 (MQ=255)
                                                   cATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGttt                                       >  1:213231/1‑93 (MQ=255)
                                                   cATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGttt                                       >  1:670693/1‑93 (MQ=255)
                                                    aTTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTa                                      >  2:6033053/1‑93 (MQ=255)
                                                    aTTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTa                                      <  1:4400537/93‑1 (MQ=255)
                                                     ttCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTaa                                     <  2:239925/93‑1 (MQ=255)
                                                       ccGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTaaaa                                   <  1:486574/93‑1 (MQ=255)
                                                       ccGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTaaaa                                   <  2:6228677/93‑1 (MQ=255)
                                                        cGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAAt                                  <  1:1680077/93‑1 (MQ=255)
                                                         ggCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATc                                 >  2:5147106/1‑93 (MQ=255)
                                                         ggCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATc                                 >  1:2475118/1‑93 (MQ=255)
                                                          gCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGCTTAAAATCg                                >  1:3369725/1‑93 (MQ=255)
                                                           cGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGa                               <  2:2518313/93‑1 (MQ=255)
                                                             aTTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAg                             <  2:2218147/93‑1 (MQ=255)
                                                             aTTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAg                             <  1:1444641/93‑1 (MQ=255)
                                                             aTTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAg                             <  2:3095175/93‑1 (MQ=255)
                                                             aTTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAg                             >  1:1022675/1‑93 (MQ=255)
                                                               tttGTAGCATTCGGTAATAGCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCg                           <  2:3848263/93‑1 (MQ=255)
                                                                 tGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATCCCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTg                         <  1:6040262/93‑1 (MQ=255)
                                                                  gTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGATTAAAAGCCAAGCGCga                        >  2:6080137/1‑93 (MQ=255)
                                                                   tAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTgag                       >  1:5338435/1‑93 (MQ=255)
                                                                     gCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCCCGCGTTTAAAATCGAAGCGTGAGaa                     <  2:2006758/93‑1 (MQ=255)
                                                                      cATTCTGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAAc                    <  1:2724649/93‑1 (MQ=255)
                                                                      cATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAAc                    <  2:4502248/93‑1 (MQ=255)
                                                                       aTTCGGTAATACCGCCGGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACa                   <  2:121896/93‑1 (MQ=255)
                                                                       aTTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACa                   <  1:2962393/93‑1 (MQ=255)
                                                                       aTTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACa                   <  2:2636581/93‑1 (MQ=255)
                                                                        ttCGGTAATACCGCCCGCGTGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAt                  >  2:3024301/1‑93 (MQ=255)
                                                                        ttCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAt                  >  1:5602825/1‑93 (MQ=255)
                                                                        ttCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAt                  >  2:4607595/1‑93 (MQ=255)
                                                                        ttCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAt                  <  1:5032821/93‑1 (MQ=255)
                                                                        ttCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAt                  >  2:5103198/1‑93 (MQ=255)
                                                                        ttCGGTAATACCGCCCGCGCGGGAGAGATCCGGATGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAt                  >  1:2809935/1‑93 (MQ=255)
                                                                         tCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATg                 >  2:3536274/1‑93 (MQ=255)
                                                                         tCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATg                 <  1:5993736/93‑1 (MQ=255)
                                                                            gTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGt              >  1:5463644/1‑93 (MQ=255)
                                                                              aaTACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTc            >  1:2679296/1‑93 (MQ=255)
                                                                               aTACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCa           >  1:1742004/1‑93 (MQ=255)
                                                                               aTACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCa           >  1:3359911/1‑93 (MQ=255)
                                                                               aTACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCa           <  2:4311643/93‑1 (MQ=255)
                                                                                tACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCAc          >  2:1169934/1‑93 (MQ=255)
                                                                                tACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCAc          >  1:6171990/1‑93 (MQ=255)
                                                                                tACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCAc          >  2:5645597/1‑93 (MQ=255)
                                                                                 aCCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCAcc         <  1:5907560/93‑1 (MQ=255)
                                                                                    gcccgcGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACccgc      <  1:2946060/93‑1 (MQ=255)
                                                                                     cccgcGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACccgcc     <  1:4126422/93‑1 (MQ=255)
                                                                                     cccgcGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACccgcc     <  1:5543848/93‑1 (MQ=255)
                                                                                      ccgcGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACccgccg    >  1:5751759/1‑93 (MQ=255)
                                                                                       cgcgTGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCcgccgc   <  1:6007201/93‑1 (MQ=255)
                                                                                        gcgcGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCgccgcc  <  1:3112763/93‑1 (MQ=255)
                                                                                           |                                                                                         
AATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGTGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCC  >  NC_000913/3869753‑3869933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGTGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCAGTG  >  NC_000913/3869752‑3869937
                                                                                            |                                                                                             
CAATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCG                                                                                              >  SRR4302537.5991832/1‑94 (MQ=60)
 AATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGG                                                                                             >  SRR4302537.5444429/1‑94 (MQ=60)
 AATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCACTCGGTAATACCGCCCGCGCGG                                                                                             >  SRR4302537.4872856/1‑94 (MQ=60)
  ATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGG                                                                                            <  SRR4302537.1382741/94‑1 (MQ=60)
  ATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGG                                                                                            <  SRR4302537.6462702/94‑1 (MQ=60)
    CGTTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAG                                                                                          <  SRR4302537.788097/94‑1 (MQ=60)
    CGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAG                                                                                          >  SRR4302537.1369983/1‑94 (MQ=60)
      GTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAG                                                                                        >  SRR4302537.2892546/1‑94 (MQ=60)
      GTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAG                                                                                        <  SRR4302537.1123064/94‑1 (MQ=60)
       TCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGA                                                                                       >  SRR4302537.6461820/1‑94 (MQ=60)
        CCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGAT                                                                                      <  SRR4302537.3311326/94‑1 (MQ=60)
         CGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATC                                                                                     <  SRR4302537.406699/94‑1 (MQ=60)
         CGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATC                                                                                     <  SRR4302537.4890609/94‑1 (MQ=60)
         CGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATC                                                                                     <  SRR4302537.1720905/94‑1 (MQ=60)
          GAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCC                                                                                    >  SRR4302537.3442071/1‑94 (MQ=60)
          GAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCC                                                                                    >  SRR4302537.4891184/1‑94 (MQ=60)
          GAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCC                                                                                    <  SRR4302537.5974333/94‑1 (MQ=60)
           AGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCG                                                                                   <  SRR4302537.3189506/94‑1 (MQ=60)
              GGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTT                                                                                <  SRR4302537.4957189/94‑1 (MQ=60)
              GGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTT                                                                                <  SRR4302537.3869038/94‑1 (MQ=60)
              GGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTGGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTT                                                                                <  SRR4302537.339278/94‑1 (MQ=60)
                  AGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAG                                                                            >  SRR4302537.1868795/1‑94 (MQ=60)
                     GCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAAT                                                                         <  SRR4302537.368551/94‑1 (MQ=60)
                      CGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATC                                                                        <  SRR4302537.5999559/94‑1 (MQ=60)
                       GGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCG                                                                       <  SRR4302537.1275618/94‑1 (MQ=60)
                       GGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCG                                                                       <  SRR4302537.3467788/94‑1 (MQ=60)
                           CAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAAT                                                                   >  SRR4302537.4780973/1‑94 (MQ=60)
                            CAGGGTGACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATA                                                                  <  SRR4302537.3165047/94‑1 (MQ=60)
                            AAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATA                                                                  <  SRR4302537.6450369/94‑1 (MQ=60)
                            AAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATA                                                                  <  SRR4302537.4128860/94‑1 (MQ=60)
                            AAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATA                                                                  >  SRR4302537.2914677/1‑94 (MQ=60)
                             AGGGGCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATAC                                                                 <  SRR4302537.6039331/94‑1 (MQ=60)
                              GGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACC                                                                >  SRR4302537.5565512/1‑94 (MQ=60)
                               GGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCA                                                               <  SRR4302537.138216/94‑1 (MQ=60)
                               GGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCA                                                               >  SRR4302537.73510/1‑94 (MQ=60)
                                  CACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCT                                                            >  SRR4302537.4609382/1‑94 (MQ=60)
                                  CACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCT                                                            <  SRR4302537.376514/94‑1 (MQ=60)
                                  CACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCT                                                            >  SRR4302537.2529691/1‑94 (MQ=60)
                                  CACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCT                                                            >  SRR4302537.223771/1‑94 (MQ=60)
                                  CACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGAGAGAGATCCGGTTGCAGAATCGAAATACCACCT                                                            <  SRR4302537.5267802/94‑1 (MQ=60)
                                     GTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCC                                                         >  SRR4302537.4247745/1‑94 (MQ=60)
                                     GTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCC                                                         >  SRR4302537.4716151/1‑94 (MQ=60)
                                       CATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTC                                                       >  SRR4302537.1066254/1‑94 (MQ=60)
                                       CATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTC                                                       >  SRR4302537.320481/1‑94 (MQ=60)
                                        ATAGGCTTCTGCCATTCCGGCGATTTTGTACCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCC                                                      >  SRR4302537.4035373/1‑94 (MQ=60)
                                           GGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGC                                                   >  SRR4302537.3264307/1‑94 (MQ=60)
                                           GGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGC                                                   >  SRR4302537.2479007/1‑94 (MQ=60)
                                            GCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCA                                                  <  SRR4302537.3166405/94‑1 (MQ=60)
                                             CTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAC                                                 <  SRR4302537.6185316/94‑1 (MQ=60)
                                             CTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAC                                                 <  SRR4302537.4947115/94‑1 (MQ=60)
                                             CTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCAC                                                 <  SRR4302537.3580127/94‑1 (MQ=60)
                                                CTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCG                                              <  SRR4302537.992071/94‑1 (MQ=60)
                                                CTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCG                                              <  SRR4302537.1979550/94‑1 (MQ=60)
                                                 TGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGT                                             <  SRR4302537.2152944/94‑1 (MQ=60)
                                                 TGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGT                                             >  SRR4302537.5829826/1‑94 (MQ=60)
                                                 TGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGT                                             <  SRR4302537.3016260/94‑1 (MQ=60)
                                                  GCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTT                                            <  SRR4302537.756483/94‑1 (MQ=60)
                                                   CCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTT                                           >  SRR4302537.675277/1‑94 (MQ=60)
                                                   CCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTT                                           >  SRR4302537.214752/1‑94 (MQ=60)
                                                   CCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTT                                           <  SRR4302537.3289887/94‑1 (MQ=60)
                                                   CCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTT                                           <  SRR4302537.5729324/94‑1 (MQ=60)
                                                     ATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAA                                         <  SRR4302537.4430904/94‑1 (MQ=60)
                                                        CCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAAT                                      <  SRR4302537.3088804/94‑1 (MQ=60)
                                                        CCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAAT                                      <  SRR4302537.2816410/94‑1 (MQ=60)
                                                        CCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAAT                                      <  SRR4302537.489915/94‑1 (MQ=60)
                                                         CGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATC                                     <  SRR4302537.1691832/94‑1 (MQ=60)
                                                         CGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATC                                     >  SRR4302537.2912681/1‑94 (MQ=60)
                                                         CGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATC                                     >  SRR4302537.2492517/1‑94 (MQ=60)
                                                          GGCGATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGCTTAAAATCG                                    >  SRR4302537.3393601/1‑94 (MQ=60)
                                                             GATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAG                                 <  SRR4302537.5537705/94‑1 (MQ=60)
                                                             GATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAG                                 >  SRR4302537.1029716/1‑94 (MQ=60)
                                                              ATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGC                                <  SRR4302537.5631769/94‑1 (MQ=60)
                                                              ATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGC                                <  SRR4302537.1363690/94‑1 (MQ=60)
                                                              ATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGC                                >  SRR4302537.3297917/1‑94 (MQ=60)
                                                              ATTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGC                                <  SRR4302537.1454704/94‑1 (MQ=60)
                                                               TTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCG                               <  SRR4302537.6107398/94‑1 (MQ=60)
                                                               TTTTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCG                               <  SRR4302537.5818904/94‑1 (MQ=60)
                                                                 TTGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTG                             >  SRR4302537.814314/1‑94 (MQ=60)
                                                                  TGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATCCCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGA                            <  SRR4302537.6081759/94‑1 (MQ=60)
                                                                  TGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGA                            >  SRR4302537.1531195/1‑94 (MQ=60)
                                                                  TGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGA                            >  SRR4302537.4755533/1‑94 (MQ=60)
                                                                  TGTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGA                            <  SRR4302537.2951294/94‑1 (MQ=60)
                                                                   GTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAG                           <  SRR4302537.4509595/94‑1 (MQ=60)
                                                                   GTAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAG                           >  SRR4302537.5375031/1‑94 (MQ=60)
                                                                    TAGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGA                          >  SRR4302537.4098004/1‑94 (MQ=60)
                                                                     AGCATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAA                         >  SRR4302537.3742893/1‑94 (MQ=60)
                                                                       CATTCTGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACA                       <  SRR4302537.2743680/94‑1 (MQ=60)
                                                                       CATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACA                       >  SRR4302537.2715803/1‑94 (MQ=60)
                                                                        ATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAT                      >  SRR4302537.583604/1‑94 (MQ=60)
                                                                        ATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAT                      >  SRR4302537.5641129/1‑94 (MQ=60)
                                                                        ATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAT                      <  SRR4302537.2983188/94‑1 (MQ=60)
                                                                        ATTCGGTAATACCGCCCGCGCGGGAGAGATCCGGATGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACAT                      >  SRR4302537.2829582/1‑94 (MQ=60)
                                                                         TTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATG                     <  SRR4302537.5067468/94‑1 (MQ=60)
                                                                         TTCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATG                     <  SRR4302537.3874750/94‑1 (MQ=60)
                                                                          TCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGC                    >  SRR4302537.5571785/1‑94 (MQ=60)
                                                                          TCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGC                    <  SRR4302537.2657693/94‑1 (MQ=60)
                                                                          TCGGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGC                    <  SRR4302537.6034902/94‑1 (MQ=60)
                                                                            GGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGT                  >  SRR4302537.57052/1‑94 (MQ=60)
                                                                            GGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGT                  >  SRR4302537.5500912/1‑94 (MQ=60)
                                                                            GGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGT                  <  SRR4302537.730738/94‑1 (MQ=60)
                                                                            GGTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGT                  >  SRR4302537.1257935/1‑94 (MQ=60)
                                                                             GTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAACTACCACCTGCCTCCAGCACGCGTTTAAAATCTAAGCGTGAGAACATGCGTT                 <  SRR4302537.2968568/94‑1 (MQ=60)
                                                                             GTAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTT                 <  SRR4302537.6050072/94‑1 (MQ=60)
                                                                              TAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTC                <  SRR4302537.5506909/94‑1 (MQ=60)
                                                                              TAATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTC                >  SRR4302537.2698002/1‑94 (MQ=60)
                                                                               AATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCA               >  SRR4302537.7775/1‑94 (MQ=60)
                                                                               AATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCA               >  SRR4302537.1754148/1‑94 (MQ=60)
                                                                               AATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCA               >  SRR4302537.3383710/1‑94 (MQ=60)
                                                                                ATACCGCCCGCGCGGGAGAGATCCGGTTGCGGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCAC              >  SRR4302537.3254714/1‑94 (MQ=60)
                                                                                ATACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCAC              >  SRR4302537.6214491/1‑94 (MQ=60)
                                                                                  ACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCC            <  SRR4302537.3377176/94‑1 (MQ=60)
                                                                                  ACCGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCC            <  SRR4302537.5948094/94‑1 (MQ=60)
                                                                                    CGCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGC          <  SRR4302537.3392398/94‑1 (MQ=60)
                                                                                     GCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCC         <  SRR4302537.4051146/94‑1 (MQ=60)
                                                                                     GCCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCC         <  SRR4302537.2966739/94‑1 (MQ=60)
                                                                                      CCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCG        >  SRR4302537.5791159/1‑94 (MQ=60)
                                                                                      CCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCG        <  SRR4302537.5581700/94‑1 (MQ=60)
                                                                                      CCCGCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCG        <  SRR4302537.4155224/94‑1 (MQ=60)
                                                                                        CGCGTGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCC      <  SRR4302537.6048474/94‑1 (MQ=60)
                                                                                         GCGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCA     <  SRR4302537.3134503/94‑1 (MQ=60)
                                                                                          CGCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCAG    >  SRR4302537.1944247/1‑94 (MQ=60)
                                                                                           GCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCAGT   <  SRR4302537.6222645/94‑1 (MQ=60)
                                                                                           GCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCAGT   >  SRR4302537.3423205/1‑94 (MQ=60)
                                                                                           GCGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCAGT   >  SRR4302537.3506629/1‑94 (MQ=60)
                                                                                            CGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCAGTG  >  SRR4302537.1570607/1‑94 (MQ=60)
                                                                                            |                                                                                             
CAATCGGTCCGAGCGGACAGTGCGGCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCATTCGGTAATACCGCCCGCGTGGGAGAGATCCGGTTGCAGAATCGAAATACCACCTGCCTCCAGCACGCGTTTAAAATCGAAGCGTGAGAACATGCGTTCACCCGCCGCCAGTG  >  NC_000913/3869752‑3869937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑