Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,872,450 +G coding (338/387 nt) yidW ← regulator protein for dgo operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,872,4501.G99.6% 899.7 / ‑4.0 258coding (338/387 nt)yidWregulator protein for dgo operon
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (131/126);  total (131/127)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 280 total aligned reads displayed.

TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536
                                                                                              |                                                                                      
tCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑t                                                                                         <  2:906901/93‑1 (MQ=255)
tCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑t                                                                                         <  1:3526861/93‑1 (MQ=255)
 cGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tg                                                                                        >  2:4277745/1‑92 (MQ=255)
 cGTTAAACGCTTCGCGGTCATGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tg                                                                                        <  1:6463756/93‑2 (MQ=255)
    tAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gccc                                                                                    <  2:455259/93‑2 (MQ=255)
      aaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCaa                                                                                   >  1:6079167/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAAc                                                                                  <  2:6213009/93‑1 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAAc                                                                                  >  2:2929456/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAAc                                                                                  >  2:1189157/1‑93 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACg                                                                                 <  1:4569927/93‑1 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACgg                                                                                >  2:1549320/1‑93 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACgg                                                                                <  2:1945348/93‑1 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACgg                                                                                >  1:2018078/1‑93 (MQ=255)
         cctccgcgNTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACgg                                                                                <  2:5912973/89‑1 (MQ=255)
          cTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCCACggg                                                                               >  2:1407133/1‑93 (MQ=255)
           ttCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGc                                                                              >  2:586938/1‑93 (MQ=255)
              gcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAAt                                                                           <  2:3616307/93‑1 (MQ=255)
              gcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAAt                                                                           >  1:1654538/1‑93 (MQ=255)
                   ccTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCg                                                                      <  1:5329658/93‑1 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     >  2:2688487/1‑93 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     >  1:5636354/1‑93 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     >  2:756200/1‑93 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGt                                                                    >  1:4237469/1‑93 (MQ=255)
                      gATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGtt                                                                   >  2:6170313/1‑93 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTcc                                                                 >  1:3077738/1‑93 (MQ=255)
                          tttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTccac                                                               <  2:782785/92‑1 (MQ=255)
                           ttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTccacc                                                              >  1:5707709/1‑93 (MQ=255)
                           ttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCAGGTTccacc                                                              >  1:2184206/1‑93 (MQ=255)
                           ttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAACCGCCGGTTccacc                                                              >  1:3837498/1‑93 (MQ=255)
                             ggCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAg                                                            <  2:1237691/93‑1 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGa                                                           <  2:1698389/93‑1 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAt                                                          <  2:6422801/93‑1 (MQ=255)
                                aaTCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAtt                                                         >  2:4963864/1‑93 (MQ=255)
                                  tcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTc                                                       <  2:3107465/93‑1 (MQ=255)
                                  tcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTc                                                       <  1:5048719/93‑1 (MQ=255)
                                    atcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGc                                                     >  1:2800656/1‑93 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    <  2:6342452/93‑1 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    >  1:244627/1‑93 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    >  2:2077566/1‑93 (MQ=255)
                                      ctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   <  1:6262710/93‑1 (MQ=255)
                                      ctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   <  1:4668540/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:2278897/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  <  2:6109639/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:3795208/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  1:4899275/1‑93 (MQ=255)
                                        agTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 >  2:2516892/2‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCg                                               >  1:5187283/1‑93 (MQ=255)
                                            cAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCt                                             >  2:1429698/1‑93 (MQ=255)
                                             aGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTc                                            <  2:6238092/93‑1 (MQ=255)
                                              gcgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCa                                           <  2:4655972/93‑1 (MQ=255)
                                              gcgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCa                                           <  2:2457189/93‑1 (MQ=255)
                                               cgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAt                                          <  2:6471321/93‑1 (MQ=255)
                                               cgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAt                                          <  1:5614152/93‑1 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGc                                       <  1:4774391/93‑1 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACAAGATTTCGCACTTCGCTCATGGc                                       >  1:5875848/1‑93 (MQ=255)
                                                   taTTCAATCTGCGCCCGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGTCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      <  2:1138924/92‑1 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  1:1961266/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCAAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  2:5009645/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  2:2348236/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  1:1708885/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     <  2:3364982/93‑1 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  1:230570/1‑93 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    >  2:3275848/1‑93 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTAGCTCATGGCACt                                    <  1:5350053/93‑1 (MQ=255)
                                                      tCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTg                                   >  2:4208626/1‑93 (MQ=255)
                                                       cAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGa                                  <  1:824332/93‑1 (MQ=255)
                                                        aaTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAt                                 <  1:2546965/93‑1 (MQ=255)
                                                        aaTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAt                                 >  1:6392721/1‑93 (MQ=255)
                                                         aTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATa                                >  2:1065438/1‑93 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               >  1:3359335/1‑93 (MQ=255)
                                                           cTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCAG‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCCCTTCGCTCATGGCACTGATAAg                              <  2:2145007/93‑1 (MQ=255)
                                                           cTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAg                              <  1:3757397/93‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             >  1:5446815/1‑93 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:4707389/93‑1 (MQ=255)
                                                             gcgcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGcc                            <  2:5547904/93‑1 (MQ=255)
                                                              cgcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCg                           <  1:2974264/93‑1 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         >  2:1471357/1‑93 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         >  1:343570/1‑93 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         >  2:5658506/1‑93 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         <  2:4638320/93‑1 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         <  2:4826601/93‑1 (MQ=255)
                                                                 cAGATCGCTGGAAGTCCCCCCCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCCCTTCGCTCATGGCACTGATAAGCCGTgg                        <  2:3898062/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       >  1:1287025/1‑93 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       <  2:2530861/93‑1 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  1:2702631/1‑93 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   <  2:1610394/93‑1 (MQ=255)
                                                                       gCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTa                  >  1:1407453/1‑93 (MQ=255)
                                                                       gCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTa                  <  2:5779469/93‑1 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  2:2895942/1‑93 (MQ=255)
                                                                          ggAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTc               >  1:1018698/1‑93 (MQ=255)
                                                                            aaGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAt             >  2:602617/1‑93 (MQ=255)
                                                                             aGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            <  2:3213920/93‑1 (MQ=255)
                                                                              atCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAttt           <  2:5309375/92‑1 (MQ=255)
                                                                               tCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAAAAGCCGTGGGTCGTAGTCAtttt          >  1:4376861/1‑93 (MQ=255)
                                                                                 gcgcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        <  1:1358046/93‑1 (MQ=255)
                                                                                  cgcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCa       >  2:2040750/1‑93 (MQ=255)
                                                                                   gcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAg      <  2:4454508/93‑1 (MQ=255)
                                                                                     gcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTTCAGCa    >  1:1528711/1‑93 (MQ=255)
                                                                                     gcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    <  2:5806200/93‑1 (MQ=255)
                                                                                      ctctGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAc   <  1:3947969/93‑1 (MQ=255)
                                                                                              |                                                                                      
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  >  NC_000913/3872357‑3872543
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TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                                <  SRR4302537.5804089/94‑1 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                                <  SRR4302537.364499/94‑1 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                                <  SRR4302537.407157/94‑1 (MQ=60)
 TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑G                                                                                              <  SRR4302537.3551808/94‑1 (MQ=60)
  CGTTAAACGCTTCGCGGTCATGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑GC                                                                                             <  SRR4302537.6507545/94‑1 (MQ=60)
   GTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑GCC                                                                                            >  SRR4302537.2304147/1‑94 (MQ=60)
      AAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAA                                                                                          >  SRR4302537.6120941/1‑94 (MQ=60)
      AAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAA                                                                                          <  SRR4302537.3940939/94‑1 (MQ=60)
      AAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAA                                                                                          >  SRR4302537.453047/1‑94 (MQ=60)
       AACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAAC                                                                                         <  SRR4302537.962580/94‑1 (MQ=60)
       AACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAAC                                                                                         >  SRR4302537.5827282/1‑94 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACG                                                                                        <  SRR4302537.4918223/94‑1 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACG                                                                                        <  SRR4302537.5657261/94‑1 (MQ=60)
        ACGCCTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCCCCGATTCAATCTGCGCCAGATCGATGGAAGTCGCGCGCTCTGCTGCCCAACG                                                                                        <  SRR4302537.5364665/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGG                                                                                       >  SRR4302537.5885575/1‑94 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGG                                                                                       <  SRR4302537.3508623/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGG                                                                                       <  SRR4302537.4601556/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGG                                                                                       >  SRR4302537.2032147/1‑94 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGG                                                                                      >  SRR4302537.4795566/1‑94 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGG                                                                                      <  SRR4302537.6402830/94‑1 (MQ=60)
             TCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAA                                                                                   <  SRR4302537.4633574/94‑1 (MQ=60)
              CGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAAT                                                                                  >  SRR4302537.1666135/1‑94 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATC                                                                                 <  SRR4302537.141356/94‑1 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATC                                                                                 >  SRR4302537.2564161/1‑94 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATC                                                                                 <  SRR4302537.3983456/94‑1 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCG                                                                                <  SRR4302537.62614/94‑1 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCG                                                                                >  SRR4302537.2823635/1‑94 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCG                                                                                <  SRR4302537.5156033/94‑1 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCC                                                                                >  SRR4302537.1948331/1‑94 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGG                                                                            <  SRR4302537.5366219/94‑1 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302537.5674927/1‑94 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGG                                                                            <  SRR4302537.4872670/94‑1 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302537.4345088/1‑94 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302537.2817653/1‑94 (MQ=60)
                     CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGT                                                                           >  SRR4302537.4266944/1‑94 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCC                                                                        >  SRR4302537.5082566/1‑94 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCC                                                                        >  SRR4302537.3099175/1‑94 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCC                                                                        <  SRR4302537.2816786/94‑1 (MQ=60)
                         TTGTTGGCAATCCTCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTAGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCA                                                                       <  SRR4302537.2055564/94‑1 (MQ=60)
                           GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACC                                                                     >  SRR4302537.5100688/1‑94 (MQ=60)
                           GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACC                                                                     >  SRR4302537.5425223/1‑94 (MQ=60)
                           GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACC                                                                     >  SRR4302537.5746769/1‑94 (MQ=60)
                           GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCAGGTTCCACC                                                                     >  SRR4302537.2199599/1‑94 (MQ=60)
                           GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAACCGCCGGTTCCACC                                                                     >  SRR4302537.3864383/1‑94 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                                   <  SRR4302537.6323709/94‑1 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                                   <  SRR4302537.2011478/94‑1 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                                   <  SRR4302537.1511472/94‑1 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                <  SRR4302537.338111/94‑1 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                >  SRR4302537.2153736/1‑94 (MQ=60)
                                   TCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCG                                                             >  SRR4302537.3447613/1‑94 (MQ=60)
                                   TCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCG                                                             <  SRR4302537.4984425/94‑1 (MQ=60)
                                   TCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCG                                                             <  SRR4302537.5083482/94‑1 (MQ=60)
                                    CATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGC                                                            >  SRR4302537.2820231/1‑94 (MQ=60)
                                     ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCA                                                           >  SRR4302537.4799820/1‑94 (MQ=60)
                                     ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCA                                                           >  SRR4302537.3668759/1‑94 (MQ=60)
                                     ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCA                                                           >  SRR4302537.246358/1‑94 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                         >  SRR4302537.4933093/1‑94 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                         <  SRR4302537.4700838/94‑1 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                         <  SRR4302537.6305701/94‑1 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                         <  SRR4302537.6187410/94‑1 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                        <  SRR4302537.1648221/94‑1 (MQ=60)
                                         CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                       >  SRR4302537.3626485/1‑94 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      >  SRR4302537.1643789/1‑94 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      >  SRR4302537.5222959/1‑94 (MQ=60)
                                               GCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAT                                                 <  SRR4302537.3247791/94‑1 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                >  SRR4302537.136957/1‑94 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                <  SRR4302537.5371788/94‑1 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                <  SRR4302537.5652536/94‑1 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                >  SRR4302537.1319054/1‑94 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                               >  SRR4302537.2487056/1‑94 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                               <  SRR4302537.5548837/94‑1 (MQ=60)
                                                  CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGC                                              <  SRR4302537.564762/94‑1 (MQ=60)
                                                  CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACAAGATTTCGCACTTCGCTCATGGC                                              >  SRR4302537.5916160/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             <  SRR4302537.837897/94‑1 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             >  SRR4302537.1974905/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             <  SRR4302537.4807353/94‑1 (MQ=60)
                                                    GATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAC                                            >  SRR4302537.232199/1‑94 (MQ=60)
                                                    GATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAC                                            >  SRR4302537.1720826/1‑94 (MQ=60)
                                                     ATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACT                                           <  SRR4302537.265336/94‑1 (MQ=60)
                                                     ATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACT                                           <  SRR4302537.3392495/94‑1 (MQ=60)
                                                      TTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTAGCTCATGGCACTG                                          <  SRR4302537.5386706/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                        >  SRR4302537.6436267/1‑94 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                        <  SRR4302537.829985/94‑1 (MQ=60)
                                                         AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                       >  SRR4302537.4362000/1‑94 (MQ=60)
                                                         AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                       <  SRR4302537.2564808/94‑1 (MQ=60)
                                                          ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAA                                      >  SRR4302537.3383130/1‑94 (MQ=60)
                                                           TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                     <  SRR4302537.270944/94‑1 (MQ=60)
                                                            CTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGC                                    <  SRR4302537.3783775/94‑1 (MQ=60)
                                                            CTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGC                                    >  SRR4302537.5483974/1‑94 (MQ=60)
                                                            CTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGC                                    >  SRR4302537.1346542/1‑94 (MQ=60)
                                                             TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                                   <  SRR4302537.2345608/94‑1 (MQ=60)
                                                             TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                                   <  SRR4302537.3783869/94‑1 (MQ=60)
                                                               CGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGT                                 >  SRR4302537.166897/1‑94 (MQ=60)
                                                               CGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGT                                 <  SRR4302537.2995094/94‑1 (MQ=60)
                                                                GCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                                <  SRR4302537.5972524/94‑1 (MQ=60)
                                                                GCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                                >  SRR4302537.345956/1‑94 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                               >  SRR4302537.1156630/1‑94 (MQ=60)
                                                                  CAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                              >  SRR4302537.1295980/1‑94 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            >  SRR4302537.2721506/1‑94 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACTGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                           <  SRR4302537.5719575/94‑1 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                           >  SRR4302537.2019840/1‑94 (MQ=60)
                                                                       CGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTA                         >  SRR4302537.1417253/1‑94 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTACCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                        >  SRR4302537.4587202/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                       <  SRR4302537.5436108/94‑1 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                       <  SRR4302537.2115971/94‑1 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                      >  SRR4302537.1025718/1‑94 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                      >  SRR4302537.6023455/1‑94 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                      >  SRR4302537.3874738/1‑94 (MQ=60)
                                                                           GGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCA                     <  SRR4302537.5672374/94‑1 (MQ=60)
                                                                           GGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCA                     >  SRR4302537.6508362/1‑94 (MQ=60)
                                                                             AAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATT                   <  SRR4302537.2231149/94‑1 (MQ=60)
                                                                              AGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT                  >  SRR4302537.1456946/1‑94 (MQ=60)
                                                                              AGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT                  >  SRR4302537.3094322/1‑94 (MQ=60)
                                                                              AGTCGCGCGCTCTGCTACCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCTCTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT                  <  SRR4302537.2912291/94‑1 (MQ=60)
                                                                               GTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAAAAGCCGTGGGTCGTAGTCATTTT                 >  SRR4302537.4407084/1‑94 (MQ=60)
                                                                                TCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTC                <  SRR4302537.1863650/94‑1 (MQ=60)
                                                                                  GCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCA              >  SRR4302537.5377614/1‑94 (MQ=60)
                                                                                  GCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCA              <  SRR4302537.1367503/94‑1 (MQ=60)
                                                                                    GCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC            <  SRR4302537.3464085/94‑1 (MQ=60)
                                                                                     CGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTTCAGCA           >  SRR4302537.1539394/1‑94 (MQ=60)
                                                                                     CGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA           <  SRR4302537.4142253/94‑1 (MQ=60)
                                                                                      GCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC          <  SRR4302537.759094/94‑1 (MQ=60)
                                                                                      GCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC          <  SRR4302537.6473850/94‑1 (MQ=60)
                                                                                       CTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC         >  SRR4302537.2939091/1‑94 (MQ=60)
                                                                                       CTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC         <  SRR4302537.3975582/94‑1 (MQ=60)
                                                                                        TCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC        >  SRR4302537.531236/1‑94 (MQ=60)
                                                                                         CTGCTGCCCAACGTGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA       <  SRR4302537.6082429/94‑1 (MQ=60)
                                                                                         CTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA       <  SRR4302537.5878749/94‑1 (MQ=60)
                                                                                           GCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATT     <  SRR4302537.524242/94‑1 (MQ=60)
                                                                                             G‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  <  SRR4302537.4036784/94‑1 (MQ=60)
                                                                                             G‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  <  SRR4302537.3513171/94‑1 (MQ=60)
                                                                                             |                                                                                              
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  >  NC_000913/3872357‑3872543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑