Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,883,918 G→A S405N (AGC→AAC)  yidC → 60 KD inner‑membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,883,9180GA96.4% 858.8 / 8.8 249S405N (AGC→AAC) yidC60 KD inner‑membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/7);  new base A (127/113);  total (129/120)
Fisher's exact test for biased strand distribution p-value = 9.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

Only 100 of 256 total aligned reads displayed.

GCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGG  >  NC_000913/3883829‑3884007
                                                                                         |                                                                                         
 cAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAg                                                                                       >  1:3976084/1‑93 (MQ=255)
   gTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGa                                                                                     >  2:2764641/1‑93 (MQ=255)
   gTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGa                                                                                     >  1:6282596/1‑93 (MQ=255)
   gTACACCTACATGGCGAAGTTGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGa                                                                                     >  2:6285230/1‑93 (MQ=255)
    tACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGaa                                                                                    <  1:3165483/93‑1 (MQ=255)
    tACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGaa                                                                                    <  2:602915/93‑1 (MQ=255)
     acacCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGaaa                                                                                   >  1:4036666/1‑93 (MQ=255)
      cacCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAat                                                                                  >  1:3997206/1‑93 (MQ=255)
        ccTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAatga                                                                                <  1:1004083/93‑1 (MQ=255)
          tCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAatgatg                                                                              <  2:3458754/93‑1 (MQ=255)
            cATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGgc                                                                            <  2:6166387/93‑1 (MQ=255)
            cATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGgc                                                                            >  2:815504/1‑93 (MQ=255)
             aTGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGCTGACAAACAGCGTATCAACCAGGAAATGATGgcg                                                                           >  1:1929848/1‑93 (MQ=255)
               ggCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCt                                                                         >  2:404024/1‑93 (MQ=255)
                  gAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGNCGCTGTa                                                                      <  1:3370007/93‑1 (MQ=255)
                     gATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACaa                                                                   <  2:1346486/93‑1 (MQ=255)
                      aTGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACaaa                                                                  <  1:4554422/93‑1 (MQ=255)
                       tGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAg                                                                 >  1:3783163/1‑93 (MQ=255)
                       tGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAg                                                                 >  2:5362001/1‑93 (MQ=255)
                       tGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAg                                                                 <  1:1319980/93‑1 (MQ=255)
                         cGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCt                                                               >  2:5529114/1‑93 (MQ=255)
                         cGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCt                                                               <  2:3512651/93‑1 (MQ=255)
                         cGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCt                                                               <  1:5315560/93‑1 (MQ=255)
                          gTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTg                                                              <  2:1555916/93‑1 (MQ=255)
                          gTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTg                                                              >  1:5536034/1‑93 (MQ=255)
                          gTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTg                                                              >  1:789865/1‑93 (MQ=255)
                           tATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTga                                                             >  1:1662733/1‑93 (MQ=255)
                            aTGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTgag                                                            >  1:2333256/1‑93 (MQ=255)
                             tGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTgaga                                                           >  1:2808054/1‑93 (MQ=255)
                              gTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGaa                                                          >  2:4280393/1‑93 (MQ=255)
                              gTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGaa                                                          <  2:3617311/93‑1 (MQ=255)
                               ttGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAg                                                         <  1:6442925/93‑1 (MQ=255)
                                tGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAgg                                                        <  2:3345116/93‑1 (MQ=255)
                                tGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAgg                                                        >  1:892679/1‑93 (MQ=255)
                                tGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAgg                                                        <  2:5983556/93‑1 (MQ=255)
                                tGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAgg                                                        >  1:515082/1‑93 (MQ=255)
                                  cAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGtt                                                      <  2:902662/93‑1 (MQ=255)
                                  cAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGtt                                                      <  1:1265788/93‑1 (MQ=255)
                                   aGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTa                                                     >  2:1041501/1‑93 (MQ=255)
                                    gCCGAAGATTCAGGCAATGCGTGTGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGACGGCGCTGTACAAAGCTGAGAAGGTTaa                                                    >  1:6474650/1‑93 (MQ=255)
                                     ccGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAAc                                                   <  2:2471773/93‑1 (MQ=255)
                                      cGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAAcc                                                  <  1:994646/93‑1 (MQ=255)
                                      cGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAAcc                                                  >  2:4730361/1‑93 (MQ=255)
                                       gAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAAccc                                                 >  1:5061236/1‑93 (MQ=255)
                                       gAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAAccc                                                 >  1:6106331/1‑93 (MQ=255)
                                        aaGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCg                                                <  2:567269/93‑1 (MQ=255)
                                         aGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGc                                               <  1:156877/93‑1 (MQ=255)
                                          gATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCt                                              <  2:2573071/93‑1 (MQ=255)
                                          gATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCt                                              <  2:3814001/93‑1 (MQ=255)
                                          gATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCt                                              <  1:723554/93‑1 (MQ=255)
                                           aTTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTg                                             >  1:276019/1‑93 (MQ=255)
                                               aGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGggcg                                         <  1:2383315/93‑1 (MQ=255)
                                                 gCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGggcggc                                       <  1:4179702/93‑1 (MQ=255)
                                                  cAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgct                                      <  1:6413935/93‑1 (MQ=255)
                                                   aaTGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgctg                                     >  2:4218533/1‑93 (MQ=255)
                                                     tGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgctgct                                   >  1:4682965/1‑93 (MQ=255)
                                                       cGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTc                                 <  2:4013894/93‑1 (MQ=255)
                                                        gTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATAGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTcc                                <  1:1176612/93‑1 (MQ=255)
                                                         tGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTccc                               >  1:1022337/1‑93 (MQ=255)
                                                         tGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTccc                               <  1:2162644/93‑1 (MQ=255)
                                                         tGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTccc                               >  1:4335594/1‑93 (MQ=255)
                                                           aGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCgc                             <  1:1490426/93‑1 (MQ=255)
                                                            gCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCgct                            >  1:4453503/1‑93 (MQ=255)
                                                              gTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCgctgc                          <  2:2181621/93‑1 (MQ=255)
                                                               tCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCgctgct                         <  2:1210308/93‑1 (MQ=255)
                                                                 tGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGa                       <  1:6315414/93‑1 (MQ=255)
                                                                     cGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCa                   >  2:4490146/1‑93 (MQ=255)
                                                                      gATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAg                  >  1:5740739/1‑93 (MQ=255)
                                                                      gATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAg                  >  2:1925011/1‑93 (MQ=255)
                                                                       aTGACAAACAGCGTATCAGTCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGa                 >  2:6299073/1‑93 (MQ=255)
                                                                       aTGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGa                 <  2:4917245/93‑1 (MQ=255)
                                                                       aTGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGa                 <  2:4390017/93‑1 (MQ=255)
                                                                       aTGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGa                 >  1:2985059/1‑93 (MQ=255)
                                                                       aTGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGa                 <  1:3208076/93‑1 (MQ=255)
                                                                        tGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGAt                >  1:5581073/1‑93 (MQ=255)
                                                                         gACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATg               >  1:2040999/1‑93 (MQ=255)
                                                                         gACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATg               >  2:5821840/1‑93 (MQ=255)
                                                                         gACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATg               >  2:771381/1‑93 (MQ=255)
                                                                           cAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGcc             <  2:1788997/93‑1 (MQ=255)
                                                                            aaaCAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCa            >  2:2396753/1‑93 (MQ=255)
                                                                             aaCAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCaa           >  2:3773146/1‑93 (MQ=255)
                                                                             aaCAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCaa           >  2:3108708/1‑93 (MQ=255)
                                                                             aaCAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCaa           >  1:2619718/1‑93 (MQ=255)
                                                                              aCAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAAt          <  1:5136666/93‑1 (MQ=255)
                                                                              aCAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAAt          >  1:5708160/1‑93 (MQ=255)
                                                                               cAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATc         <  1:3500490/93‑1 (MQ=255)
                                                                               cAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATc         <  2:4947932/93‑1 (MQ=255)
                                                                               cAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATc         <  1:1996697/93‑1 (MQ=255)
                                                                                aGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCt        >  2:1740570/1‑93 (MQ=255)
                                                                                aGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCt        >  1:209094/1‑93 (MQ=255)
                                                                                 gCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCtt       >  2:2899715/1‑93 (MQ=255)
                                                                                   gTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTcc     <  1:3016766/93‑1 (MQ=255)
                                                                                   gTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTcc     >  1:5957556/1‑93 (MQ=255)
                                                                                     aTCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTg   >  1:6177851/1‑93 (MQ=255)
                                                                                     aTCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTg   <  2:4565315/93‑1 (MQ=255)
                                                                                     aTCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTg   <  2:3174818/93‑1 (MQ=255)
                                                                                     aTCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTg   >  2:2982323/1‑93 (MQ=255)
                                                                                      tCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTgg  >  2:1416969/1‑93 (MQ=255)
                                                                                      tCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTgg  >  1:5387149/1‑93 (MQ=255)
                                                                                      tCAACCAGGAAATGATGGCCCTGTACAAAGCTGAGAAGGTTAACCCTCTGGGCGCCTGCTTCCCGCTGCTGCTCCAGATGCCAATCTTCCTgg  <  2:4832162/93‑1 (MQ=255)
                                                                                         |                                                                                         
GCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGG  >  NC_000913/3883829‑3884007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTT  >  NC_000913/3883825‑3884011
                                                                                             |                                                                                             
AAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAA                                                                                               >  SRR4302537.6230155/1‑94 (MQ=60)
 AGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAAC                                                                                              <  SRR4302537.3256175/94‑1 (MQ=60)
   CGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCA                                                                                            >  SRR4302537.1055792/1‑94 (MQ=60)
    GCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAG                                                                                           <  SRR4302537.6097338/94‑1 (MQ=60)
    GCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAG                                                                                           >  SRR4302537.4003911/1‑94 (MQ=60)
     CAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGG                                                                                          <  SRR4302537.3722948/94‑1 (MQ=60)
      AGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGA                                                                                         >  SRR4302537.6325679/1‑94 (MQ=60)
      AGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGA                                                                                         >  SRR4302537.1392539/1‑94 (MQ=60)
        TACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAA                                                                                       >  SRR4302537.4064915/1‑94 (MQ=60)
        TACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAA                                                                                       <  SRR4302537.3187712/94‑1 (MQ=60)
         ACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAAT                                                                                      >  SRR4302537.4025189/1‑94 (MQ=60)
         ACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAAT                                                                                      <  SRR4302537.3554712/94‑1 (MQ=60)
          CACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATG                                                                                     <  SRR4302537.5162937/94‑1 (MQ=60)
          CACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATG                                                                                     >  SRR4302537.2789611/1‑94 (MQ=60)
           ACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGA                                                                                    >  SRR4302537.927800/1‑94 (MQ=60)
           ACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGA                                                                                    >  SRR4302537.4573932/1‑94 (MQ=60)
           ACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGAGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGA                                                                                    >  SRR4302537.4007523/1‑94 (MQ=60)
            CCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGAT                                                                                   <  SRR4302537.1011015/94‑1 (MQ=60)
             CTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATG                                                                                  >  SRR4302537.5179913/1‑94 (MQ=60)
             CTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATG                                                                                  >  SRR4302537.1887487/1‑94 (MQ=60)
             CTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATG                                                                                  <  SRR4302537.1928155/94‑1 (MQ=60)
             CTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATG                                                                                  <  SRR4302537.4260035/94‑1 (MQ=60)
               CCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGC                                                                                <  SRR4302537.1277692/94‑1 (MQ=60)
                CATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGCTGACAAACAGCGTATCAACCAGGAAATGATGGCG                                                                               >  SRR4302537.1943243/1‑94 (MQ=60)
                CATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCG                                                                               <  SRR4302537.5860148/94‑1 (MQ=60)
                CATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGCCTGGGCGATGACAAACAGCGTATCAACCAGGACATGATGGCC                                                                               >  SRR4302537.6344521/1‑94 (MQ=60)
                 ATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGC                                                                              >  SRR4302537.6506508/1‑94 (MQ=60)
                  TGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCT                                                                             >  SRR4302537.5060615/1‑94 (MQ=60)
                      GAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGNCGCTGTAC                                                                         <  SRR4302537.3393886/94‑1 (MQ=60)
                      GAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTAC                                                                         >  SRR4302537.2986658/1‑94 (MQ=60)
                       AAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACA                                                                        <  SRR4302537.207330/94‑1 (MQ=60)
                         GATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAA                                                                      <  SRR4302537.5195/94‑1 (MQ=60)
                          ATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAG                                                                     <  SRR4302537.4585944/94‑1 (MQ=60)
                          ATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAG                                                                     >  SRR4302537.5766859/1‑94 (MQ=60)
                          ATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAG                                                                     >  SRR4302537.3809723/1‑94 (MQ=60)
                           TGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGC                                                                    <  SRR4302537.1329181/94‑1 (MQ=60)
                            GCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCT                                                                   >  SRR4302537.1004087/1‑94 (MQ=60)
                            GCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCT                                                                   <  SRR4302537.5528689/94‑1 (MQ=60)
                             CGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTG                                                                  <  SRR4302537.284477/94‑1 (MQ=60)
                             CGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTG                                                                  <  SRR4302537.5352064/94‑1 (MQ=60)
                             CGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTG                                                                  >  SRR4302537.795286/1‑94 (MQ=60)
                             CGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTG                                                                  >  SRR4302537.5573823/1‑94 (MQ=60)
                              GTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGA                                                                 >  SRR4302537.1674382/1‑94 (MQ=60)
                              GTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGA                                                                 >  SRR4302537.3200403/1‑94 (MQ=60)
                               TATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAG                                                                >  SRR4302537.2349705/1‑94 (MQ=60)
                               TATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAG                                                                >  SRR4302537.1972491/1‑94 (MQ=60)
                                ATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGA                                                               >  SRR4302537.2827687/1‑94 (MQ=60)
                                  GTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAG                                                             >  SRR4302537.1959601/1‑94 (MQ=60)
                                   TTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGG                                                            <  SRR4302537.4179809/94‑1 (MQ=60)
                                   TTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGG                                                            >  SRR4302537.898839/1‑94 (MQ=60)
                                   TTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGG                                                            <  SRR4302537.6486643/94‑1 (MQ=60)
                                   TTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGG                                                            >  SRR4302537.518625/1‑94 (MQ=60)
                                     GCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTT                                                          <  SRR4302537.3385475/94‑1 (MQ=60)
                                      CAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTA                                                         <  SRR4302537.1274590/94‑1 (MQ=60)
                                      CAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTA                                                         >  SRR4302537.3144233/1‑94 (MQ=60)
                                       AGCCGAAGATTCAGGCAATGCGTGTGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGACGGCGCTGTACAAAGCTGAGAAGGTTAA                                                        >  SRR4302537.6518597/1‑94 (MQ=60)
                                       AGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAA                                                        >  SRR4302537.3839688/1‑94 (MQ=60)
                                         CCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACC                                                      >  SRR4302537.70046/1‑94 (MQ=60)
                                          CGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCC                                                     <  SRR4302537.1001514/94‑1 (MQ=60)
                                          CGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCC                                                     <  SRR4302537.4479136/94‑1 (MQ=60)
                                          CGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCC                                                     >  SRR4302537.6148288/1‑94 (MQ=60)
                                          CGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCC                                                     >  SRR4302537.5096080/1‑94 (MQ=60)
                                          CGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCC                                                     >  SRR4302537.1662721/1‑94 (MQ=60)
                                            AAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGC                                                   >  SRR4302537.3249041/1‑94 (MQ=60)
                                             AGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCT                                                  <  SRR4302537.157994/94‑1 (MQ=60)
                                              GATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTG                                                 >  SRR4302537.3495940/1‑94 (MQ=60)
                                              GATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTG                                                 <  SRR4302537.5319299/94‑1 (MQ=60)
                                              GATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTG                                                 >  SRR4302537.277951/1‑94 (MQ=60)
                                              GATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTG                                                 <  SRR4302537.728495/94‑1 (MQ=60)
                                                   AGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGG                                            >  SRR4302537.6518278/1‑94 (MQ=60)
                                                   AGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGG                                            <  SRR4302537.2400105/94‑1 (MQ=60)
                                                   AGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCAGCTGGGCGG                                            >  SRR4302537.1516997/1‑94 (MQ=60)
                                                   AGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGCAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGG                                            <  SRR4302537.4221849/94‑1 (MQ=60)
                                                    GGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGC                                           >  SRR4302537.6242662/1‑94 (MQ=60)
                                                     GCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCT                                          <  SRR4302537.4208877/94‑1 (MQ=60)
                                                     GCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCT                                          >  SRR4302537.682874/1‑94 (MQ=60)
                                                      CAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTG                                         <  SRR4302537.6457561/94‑1 (MQ=60)
                                                      CAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTG                                         >  SRR4302537.5096605/1‑94 (MQ=60)
                                                       AATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGC                                        >  SRR4302537.251105/1‑94 (MQ=60)
                                                       AATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGC                                        >  SRR4302537.4962680/1‑94 (MQ=60)
                                                        ATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCT                                       >  SRR4302537.4715360/1‑94 (MQ=60)
                                                         TGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTT                                      >  SRR4302537.2056619/1‑94 (MQ=60)
                                                           CGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCC                                    <  SRR4302537.4672792/94‑1 (MQ=60)
                                                           CGTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCC                                    >  SRR4302537.4053003/1‑94 (MQ=60)
                                                            GTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCC                                   >  SRR4302537.4365699/1‑94 (MQ=60)
                                                            GTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCC                                   <  SRR4302537.2049090/94‑1 (MQ=60)
                                                            GTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCC                                   >  SRR4302537.859287/1‑94 (MQ=60)
                                                            GTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCC                                   >  SRR4302537.1029377/1‑94 (MQ=60)
                                                            GTGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATAGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCC                                   <  SRR4302537.1184793/94‑1 (MQ=60)
                                                             TGAGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCG                                  <  SRR4302537.2177884/94‑1 (MQ=60)
                                                               AGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCT                                <  SRR4302537.1500804/94‑1 (MQ=60)
                                                               AGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCT                                <  SRR4302537.6252625/94‑1 (MQ=60)
                                                               AGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCT                                >  SRR4302537.4484260/1‑94 (MQ=60)
                                                               AGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCT                                <  SRR4302537.3006225/94‑1 (MQ=60)
                                                               AGCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCT                                <  SRR4302537.5531383/94‑1 (MQ=60)
                                                                GCGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTG                               <  SRR4302537.538402/94‑1 (MQ=60)
                                                                 CGTCTGGGCGATGACAAACAGCTTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGC                              >  SRR4302537.6464463/1‑94 (MQ=60)
                                                                 CGTCTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGC                              >  SRR4302537.2345881/1‑94 (MQ=60)
                                                                  GTCTGAGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCT                             <  SRR4302537.6370940/94‑1 (MQ=60)
                                                                    CTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGA                           >  SRR4302537.1023996/1‑94 (MQ=60)
                                                                    CTGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGA                           >  SRR4302537.1206041/1‑94 (MQ=60)
                                                                     TGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGAT                          <  SRR4302537.6358637/94‑1 (MQ=60)
                                                                     TGGGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGAT                          <  SRR4302537.6204170/94‑1 (MQ=60)
                                                                       GGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCC                        >  SRR4302537.1564129/1‑94 (MQ=60)
                                                                       GGCGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCC                        <  SRR4302537.4025577/94‑1 (MQ=60)
                                                                         CGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAG                      <  SRR4302537.131045/94‑1 (MQ=60)
                                                                         CGATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAG                      >  SRR4302537.5780046/1‑94 (MQ=60)
                                                                          GATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGA                     >  SRR4302537.3005917/1‑94 (MQ=60)
                                                                           ATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGAT                    <  SRR4302537.3230630/94‑1 (MQ=60)
                                                                           ATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGAT                    <  SRR4302537.3854608/94‑1 (MQ=60)
                                                                           ATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGAT                    <  SRR4302537.2540317/94‑1 (MQ=60)
                                                                           ATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGAT                    >  SRR4302537.5619220/1‑94 (MQ=60)
                                                                           ATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGAT                    <  SRR4302537.2773534/94‑1 (MQ=60)
                                                                           ATGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGAT                    >  SRR4302537.2326871/1‑94 (MQ=60)
                                                                            TGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATG                   >  SRR4302537.2055253/1‑94 (MQ=60)
                                                                            TGACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATG                   <  SRR4302537.612967/94‑1 (MQ=60)
                                                                             GACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGC                  <  SRR4302537.2375077/94‑1 (MQ=60)
                                                                              ACAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCTCTGGGCGGCTGCTTCCCGCTGCTTATCCAGATGCC                 >  SRR4302537.6377302/1‑94 (MQ=60)
                                                                               CAAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCA                <  SRR4302537.4520930/94‑1 (MQ=60)
                                                                                AAACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAA               >  SRR4302537.2638030/1‑94 (MQ=60)
                                                                                 AACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAAT              >  SRR4302537.5747222/1‑94 (MQ=60)
                                                                                  ACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATC             >  SRR4302537.3060529/1‑94 (MQ=60)
                                                                                  ACAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATC             <  SRR4302537.5172063/94‑1 (MQ=60)
                                                                                   CAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCT            <  SRR4302537.3525256/94‑1 (MQ=60)
                                                                                   CAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCT            >  SRR4302537.779437/1‑94 (MQ=60)
                                                                                   CAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCT            <  SRR4302537.2010579/94‑1 (MQ=60)
                                                                                   CAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCT            >  SRR4302537.210597/1‑94 (MQ=60)
                                                                                   CAGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCT            <  SRR4302537.5809069/94‑1 (MQ=60)
                                                                                   CAGCGTATCAACCAGGAAATGATGACGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCT            <  SRR4302537.5613825/94‑1 (MQ=60)
                                                                                    AGCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTT           >  SRR4302537.2105881/1‑94 (MQ=60)
                                                                                     GCGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTC          >  SRR4302537.1175100/1‑94 (MQ=60)
                                                                                      CGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCC         >  SRR4302537.5720419/1‑94 (MQ=60)
                                                                                      CGTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCC         >  SRR4302537.5998447/1‑94 (MQ=60)
                                                                                       GTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCT        <  SRR4302537.822532/94‑1 (MQ=60)
                                                                                       GTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCT        >  SRR4302537.2782576/1‑94 (MQ=60)
                                                                                       GTATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCT        <  SRR4302537.3037795/94‑1 (MQ=60)
                                                                                        TATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTG       >  SRR4302537.6220398/1‑94 (MQ=60)
                                                                                        TATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTG       <  SRR4302537.5679759/94‑1 (MQ=60)
                                                                                         ATCAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGG      >  SRR4302537.5423967/1‑94 (MQ=60)
                                                                                           CAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCG    <  SRR4302537.1479638/94‑1 (MQ=60)
                                                                                           CAACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCG    <  SRR4302537.3725184/94‑1 (MQ=60)
                                                                                            AACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGT   >  SRR4302537.452352/1‑94 (MQ=60)
                                                                                             GCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTT  >  SRR4302537.2275827/1‑94 (MQ=60)
                                                                                             ACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTT  <  SRR4302537.4237215/94‑1 (MQ=60)
                                                                                             ACCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTT  <  SRR4302537.6086314/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTT  >  NC_000913/3883825‑3884011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑