Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,913,922 A→G V361A (GTT→GCT)  atpD ← membrane‑bound ATP synthase, F1 sector, beta‑subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,913,9220AG98.5% 895.7 / 0.9 265V361A (GTT→GCT) atpDmembrane‑bound ATP synthase, F1 sector, beta‑subunit
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base G (141/119);  total (145/120)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

Only 100 of 272 total aligned reads displayed.

CCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATA  >  NC_000913/3913833‑3914014
                                                                                         |                                                                                            
 caccaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGcc                                                                                          <  2:2028570/93‑1 (MQ=255)
  cccaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCa                                                                                         <  2:1020517/92‑1 (MQ=255)
  accaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCa                                                                                         <  1:1203999/93‑1 (MQ=255)
  accaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCa                                                                                         <  1:1773693/93‑1 (MQ=255)
  accaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCa                                                                                         >  2:1343245/1‑93 (MQ=255)
   ccaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAc                                                                                        >  2:4569157/1‑93 (MQ=255)
   ccaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAc                                                                                        <  2:3714303/93‑1 (MQ=255)
   ccaGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAc                                                                                        >  2:2529304/1‑93 (MQ=255)
     aGTTTGTCTTCTTCGGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAcgc                                                                                      >  2:3395339/1‑93 (MQ=255)
     aGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAcgc                                                                                      <  2:872205/93‑1 (MQ=255)
      gTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAcgcg                                                                                     >  1:1161415/1‑93 (MQ=255)
      gTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAcgcg                                                                                     <  1:3612140/93‑1 (MQ=255)
       tttGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAcgcgc                                                                                    <  1:4368737/93‑1 (MQ=255)
        ttGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAcgcgcg                                                                                   <  2:5251667/93‑1 (MQ=255)
         tGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCgg                                                                                  >  2:4813528/1‑93 (MQ=255)
          gTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGgt                                                                                 <  1:2546686/93‑1 (MQ=255)
           tcttctTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGgtg                                                                                <  1:4930680/93‑1 (MQ=255)
            cttcttCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGgtgt                                                                               >  2:292745/1‑93 (MQ=255)
             ttcttcAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTc                                                                              >  2:1655719/1‑93 (MQ=255)
             ttcttcAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTc                                                                              <  2:5637460/93‑1 (MQ=255)
               cttcAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGt                                                                            <  2:4087385/93‑1 (MQ=255)
                ttcAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTa                                                                           >  1:6385724/1‑93 (MQ=255)
                ttcAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTa                                                                           >  1:5835320/1‑93 (MQ=255)
                ttcAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTa                                                                           >  2:6055369/1‑93 (MQ=255)
                 tcAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAg                                                                          >  1:2761387/1‑93 (MQ=255)
                    gACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAgtgt                                                                       >  1:4465836/1‑93 (MQ=255)
                      cAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTc                                                                     <  1:2421039/93‑1 (MQ=255)
                      cAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTc                                                                     >  1:1349043/1‑93 (MQ=255)
                       aGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTcc                                                                    <  2:143944/93‑1 (MQ=255)
                        gTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCt                                                                   >  1:882423/1‑93 (MQ=255)
                        gTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCt                                                                   >  1:4209758/1‑93 (MQ=255)
                         ttCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTg                                                                  >  1:3946572/1‑93 (MQ=255)
                           cATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGac                                                                <  1:5471463/93‑1 (MQ=255)
                              ccATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGaccaa                                                             <  2:5104275/93‑1 (MQ=255)
                                aTACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGAGTCGTAGTGTTCCTGtgcaacc                                                           >  2:4869511/1‑93 (MQ=255)
                                 tACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGaccaacca                                                          >  1:6008333/1‑93 (MQ=255)
                                 tACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGaccaacca                                                          >  2:3542958/1‑93 (MQ=255)
                                  aCCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAaccac                                                         >  1:4762679/1‑93 (MQ=255)
                                    ccAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAAccagca                                                       >  1:4814360/1‑93 (MQ=255)
                                    ccAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAAccacca                                                       >  2:2076643/1‑93 (MQ=255)
                                     cAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAg                                                      <  2:2571399/93‑1 (MQ=255)
                                      aGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGc                                                     <  2:3028948/93‑1 (MQ=255)
                                       ggATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCg                                                    <  1:158567/93‑1 (MQ=255)
                                       ggATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCg                                                    >  1:3137136/1‑93 (MQ=255)
                                        gATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCgg                                                   <  1:4695580/93‑1 (MQ=255)
                                         aTGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCggg                                                  <  1:5296541/93‑1 (MQ=255)
                                          tGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGt                                                 <  2:6157510/93‑1 (MQ=255)
                                          tGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGt                                                 >  2:2843722/1‑93 (MQ=255)
                                           ggCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTc                                                <  2:3262248/93‑1 (MQ=255)
                                           ggCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTc                                                <  2:3773896/93‑1 (MQ=255)
                                            gCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTcc                                               <  1:2709083/93‑1 (MQ=255)
                                              gatgatGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAg                                             >  1:4523596/1‑93 (MQ=255)
                                              gatgatGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAg                                             <  2:211313/93‑1 (MQ=255)
                                               atgatgTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGc                                            <  2:5880271/93‑1 (MQ=255)
                                               atgatgTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGc                                            <  2:1901645/93‑1 (MQ=255)
                                                tgatgTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCt                                           <  1:2340791/93‑1 (MQ=255)
                                                   tgTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGAc                                        <  2:2378046/93‑1 (MQ=255)
                                                     tCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACgg                                      >  1:215910/1‑93 (MQ=255)
                                                     tCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACgg                                      >  1:1938650/1‑93 (MQ=255)
                                                     tCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACgg                                      >  1:2978966/1‑93 (MQ=255)
                                                      cTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGc                                     <  1:129110/93‑1 (MQ=255)
                                                      cTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGc                                     >  1:790541/1‑93 (MQ=255)
                                                      cTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGc                                     <  2:2644440/93‑1 (MQ=255)
                                                       tttCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCt                                    >  2:2440042/1‑93 (MQ=255)
                                                          cAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCtggt                                 <  2:5749575/93‑1 (MQ=255)
                                                           aGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCtggtg                                <  2:6243698/93‑1 (MQ=255)
                                                           aGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGATGACGGCtggtg                                >  1:1397934/1‑93 (MQ=255)
                                                            gTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCtggtgg                               >  2:3819526/1‑93 (MQ=255)
                                                            gTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCtggtgg                               >  1:5051395/1‑93 (MQ=255)
                                                            gTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCtggtgg                               >  2:6334798/1‑93 (MQ=255)
                                                                cTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTc                           <  1:5798076/93‑1 (MQ=255)
                                                                 tGATATCGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTcc                          >  2:4720841/1‑93 (MQ=255)
                                                                 tGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTcc                          <  2:578365/93‑1 (MQ=255)
                                                                  taTAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCGCCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCa                         <  1:5159483/92‑1 (MQ=255)
                                                                  gATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCa                         <  1:5889934/93‑1 (MQ=255)
                                                                   aTAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAg                        <  1:4315140/93‑1 (MQ=255)
                                                                   aTAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAg                        <  1:2720497/93‑1 (MQ=255)
                                                                   aTAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAg                        <  2:1090607/93‑1 (MQ=255)
                                                                   aTAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAg                        <  2:1298661/93‑1 (MQ=255)
                                                                    tAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAgg                       >  1:3092787/1‑92 (MQ=255)
                                                                    tAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGc                       <  1:4247730/93‑1 (MQ=255)
                                                                     accGTTGCAGAATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCg                      <  2:3015219/91‑1 (MQ=255)
                                                                     aaCGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCg                      <  1:6039592/93‑1 (MQ=255)
                                                                      aCGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCgg                     >  1:1032990/1‑93 (MQ=255)
                                                                        gTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGCCGGCTGGTGGAGTCCACCGGGt                   >  2:5422473/1‑93 (MQ=255)
                                                                         ttGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTc                  <  1:3731102/93‑1 (MQ=255)
                                                                            cAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAAc               <  1:4154797/93‑1 (MQ=255)
                                                                            cAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAAc               <  2:5323618/93‑1 (MQ=255)
                                                                             aGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACg              <  2:5589899/93‑1 (MQ=255)
                                                                             aGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACg              <  2:4478687/93‑1 (MQ=255)
                                                                               gATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGc            <  2:5568637/93‑1 (MQ=255)
                                                                               gATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGc            <  2:3979991/93‑1 (MQ=255)
                                                                               gATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGc            <  1:6155928/93‑1 (MQ=255)
                                                                                aTGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACTGCTGGTGGAGTCCAGCGGGTCAACGGcc           >  1:6263309/1‑93 (MQ=255)
                                                                                 tGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCg          <  2:4652416/93‑1 (MQ=255)
                                                                                 tGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCg          <  2:3212127/93‑1 (MQ=255)
                                                                                  ggACTGATCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCgg         >  1:6330772/1‑93 (MQ=255)
                                                                                  ggACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCgg         <  2:4396932/93‑1 (MQ=255)
                                                                                     cTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCCACGGCCGGGtg      >  1:2307346/1‑92 (MQ=255)
                                                                                         aCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATa  <  1:3859641/93‑1 (MQ=255)
                                                                                         |                                                                                            
CCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATA  >  NC_000913/3913833‑3914014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATAC  >  NC_000913/3913829‑3914015
                                                                                             |                                                                                             
GCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAG                                                                                               <  SRR4302537.4846768/94‑1 (MQ=60)
GCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAG                                                                                               <  SRR4302537.4086039/94‑1 (MQ=60)
GCTACCACCAGTTTGTCTTCTTCAGACAATTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAG                                                                                               >  SRR4302537.4760252/1‑94 (MQ=60)
  TACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCG                                                                                             <  SRR4302537.3940438/94‑1 (MQ=60)
    CCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCC                                                                                           >  SRR4302537.481619/1‑94 (MQ=60)
     CACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCA                                                                                          >  SRR4302537.5111880/1‑94 (MQ=60)
      ACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAC                                                                                         <  SRR4302537.1212373/94‑1 (MQ=60)
      ACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAC                                                                                         <  SRR4302537.1786046/94‑1 (MQ=60)
      ACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCAC                                                                                         >  SRR4302537.132727/1‑94 (MQ=60)
       CCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACG                                                                                        >  SRR4302537.4234348/1‑94 (MQ=60)
       CCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACG                                                                                        <  SRR4302537.2088649/94‑1 (MQ=60)
       CCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACG                                                                                        <  SRR4302537.4764780/94‑1 (MQ=60)
       CCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACG                                                                                        <  SRR4302537.3396512/94‑1 (MQ=60)
         AGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCG                                                                                      <  SRR4302537.3014737/94‑1 (MQ=60)
         AGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCG                                                                                      <  SRR4302537.2541479/94‑1 (MQ=60)
         AGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCG                                                                                      >  SRR4302537.1169492/1‑94 (MQ=60)
          GTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGC                                                                                     <  SRR4302537.3637608/94‑1 (MQ=60)
          GTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGC                                                                                     <  SRR4302537.6427265/94‑1 (MQ=60)
           TTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCG                                                                                    <  SRR4302537.4398930/94‑1 (MQ=60)
              GTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTG                                                                                 <  SRR4302537.2564528/94‑1 (MQ=60)
              GTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTG                                                                                 >  SRR4302537.6358833/1‑94 (MQ=60)
               TCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGT                                                                                <  SRR4302537.3137604/94‑1 (MQ=60)
               TCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGT                                                                                <  SRR4302537.4964666/94‑1 (MQ=60)
                 TTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCG                                                                              >  SRR4302537.5933335/1‑94 (MQ=60)
                  TCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGT                                                                             >  SRR4302537.4778408/1‑94 (MQ=60)
                   CTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTA                                                                            >  SRR4302537.6429247/1‑94 (MQ=60)
                   CTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTA                                                                            >  SRR4302537.5875314/1‑94 (MQ=60)
                    TTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAG                                                                           >  SRR4302537.4965298/1‑94 (MQ=60)
                    TTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAG                                                                           >  SRR4302537.6148303/1‑94 (MQ=60)
                    TTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAG                                                                           >  SRR4302537.2780655/1‑94 (MQ=60)
                    TTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAG                                                                           <  SRR4302537.3933571/94‑1 (MQ=60)
                     TCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGT                                                                          >  SRR4302537.1381028/1‑94 (MQ=60)
                      CAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTG                                                                         >  SRR4302537.221207/1‑94 (MQ=60)
                      CAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTG                                                                         >  SRR4302537.3343772/1‑94 (MQ=60)
                       AGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGT                                                                        >  SRR4302537.4496722/1‑94 (MQ=60)
                       AGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGT                                                                        >  SRR4302537.5504475/1‑94 (MQ=60)
                         ACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTC                                                                      >  SRR4302537.1358444/1‑94 (MQ=60)
                          CAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCC                                                                     <  SRR4302537.2438098/94‑1 (MQ=60)
                          CAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCC                                                                     <  SRR4302537.5719471/94‑1 (MQ=60)
                           AGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCT                                                                    >  SRR4302537.888493/1‑94 (MQ=60)
                           AGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCT                                                                    >  SRR4302537.4239125/1‑94 (MQ=60)
                            GTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTG                                                                   >  SRR4302537.3974177/1‑94 (MQ=60)
                            GTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTG                                                                   <  SRR4302537.867956/94‑1 (MQ=60)
                               CATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACC                                                                <  SRR4302537.5508793/94‑1 (MQ=60)
                               CATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACC                                                                >  SRR4302537.273833/1‑94 (MQ=60)
                               CATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACC                                                                <  SRR4302537.6066224/94‑1 (MQ=60)
                                    ATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCA                                                           >  SRR4302537.6049618/1‑94 (MQ=60)
                                     TACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCAC                                                          >  SRR4302537.4300679/1‑94 (MQ=60)
                                     TACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCAC                                                          >  SRR4302537.4795572/1‑94 (MQ=60)
                                     TACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCAC                                                          >  SRR4302537.4950570/1‑94 (MQ=60)
                                       CCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCAGCA                                                        >  SRR4302537.4847592/1‑94 (MQ=60)
                                         CAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGC                                                      <  SRR4302537.5238483/94‑1 (MQ=60)
                                         CAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGC                                                      <  SRR4302537.2879876/94‑1 (MQ=60)
                                         CAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGC                                                      <  SRR4302537.3136932/94‑1 (MQ=60)
                                          AGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCG                                                     >  SRR4302537.3159156/1‑94 (MQ=60)
                                          AGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCG                                                     >  SRR4302537.3681621/1‑94 (MQ=60)
                                          AGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCG                                                     >  SRR4302537.4098352/1‑94 (MQ=60)
                                           GGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGG                                                    >  SRR4302537.2668402/1‑94 (MQ=60)
                                           GGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGG                                                    <  SRR4302537.159694/94‑1 (MQ=60)
                                            GATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGG                                                   <  SRR4302537.4728066/94‑1 (MQ=60)
                                             ATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGT                                                  <  SRR4302537.5332930/94‑1 (MQ=60)
                                             ATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGT                                                  >  SRR4302537.604791/1‑94 (MQ=60)
                                             AAGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGT                                                  <  SRR4302537.2923724/94‑1 (MQ=60)
                                              TGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTC                                                 >  SRR4302537.6191661/1‑94 (MQ=60)
                                                GCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCA                                               <  SRR4302537.2728002/94‑1 (MQ=60)
                                                 CGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAG                                              <  SRR4302537.5923770/94‑1 (MQ=60)
                                                 CGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAG                                              >  SRR4302537.1273894/1‑94 (MQ=60)
                                                 CGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAG                                              >  SRR4302537.4554892/1‑94 (MQ=60)
                                                  GATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGC                                             >  SRR4302537.1070260/1‑94 (MQ=60)
                                                  GATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGC                                             >  SRR4302537.2759930/1‑94 (MQ=60)
                                                  GATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGC                                             >  SRR4302537.1489434/1‑94 (MQ=60)
                                                   ATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCT                                            <  SRR4302537.2328671/94‑1 (MQ=60)
                                                   ATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCT                                            >  SRR4302537.863246/1‑94 (MQ=60)
                                                   ATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCT                                            <  SRR4302537.118876/94‑1 (MQ=60)
                                                    TGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTG                                           <  SRR4302537.2357289/94‑1 (MQ=60)
                                                    TGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTG                                           >  SRR4302537.5056753/1‑94 (MQ=60)
                                                     GATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGA                                          >  SRR4302537.6335464/1‑94 (MQ=60)
                                                      ATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGAC                                         >  SRR4302537.5897646/1‑94 (MQ=60)
                                                       TGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACG                                        <  SRR4302537.2405721/94‑1 (MQ=60)
                                                        GTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGagagcagctgaggg                                       >  SRR4302537.4326967/1‑80 (MQ=60)
                                                        GTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGG                                       >  SRR4302537.2999807/1‑94 (MQ=60)
                                                        GTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGG                                       >  SRR4302537.1952131/1‑94 (MQ=60)
                                                        GTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGG                                       >  SRR4302537.217452/1‑94 (MQ=60)
                                                         TCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGC                                      >  SRR4302537.795969/1‑94 (MQ=60)
                                                          CTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCT                                     >  SRR4302537.1193284/1‑94 (MQ=60)
                                                          CTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCT                                     <  SRR4302537.130027/94‑1 (MQ=60)
                                                           TTTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTG                                    <  SRR4302537.1369/94‑1 (MQ=60)
                                                            TTCAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGG                                   >  SRR4302537.5216190/1‑94 (MQ=60)
                                                              CAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTG                                 >  SRR4302537.2157258/1‑94 (MQ=60)
                                                              CAGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGATGACGGCTGGTG                                 >  SRR4302537.1407677/1‑94 (MQ=60)
                                                               AGTTCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGG                                >  SRR4302537.5086174/1‑94 (MQ=60)
                                                                  TCCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGC                             >  SRR4302537.6208046/1‑94 (MQ=60)
                                                                   CCTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTC                            <  SRR4302537.4369784/94‑1 (MQ=60)
                                                                    CTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCC                           <  SRR4302537.6234078/94‑1 (MQ=60)
                                                                    CTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCC                           <  SRR4302537.5837826/94‑1 (MQ=60)
                                                                    CTGATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCC                           >  SRR4302537.4350022/1‑94 (MQ=60)
                                                                      TATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCGCCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAG                         <  SRR4302537.5195041/94‑1 (MQ=60)
                                                                      GATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAG                         <  SRR4302537.5930349/94‑1 (MQ=60)
                                                                       ATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGG                        >  SRR4302537.3114361/1‑94 (MQ=60)
                                                                       ATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGC                        <  SRR4302537.4345134/94‑1 (MQ=60)
                                                                       ATAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGC                        <  SRR4302537.2739495/94‑1 (MQ=60)
                                                                        TAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCG                       >  SRR4302537.2186747/1‑94 (MQ=60)
                                                                        TAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCG                       >  SRR4302537.4007848/1‑94 (MQ=60)
                                                                        TAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCG                       <  SRR4302537.4277250/94‑1 (MQ=60)
                                                                        TAACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCG                       <  SRR4302537.4711003/94‑1 (MQ=60)
                                                                         AACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCTTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGG                      <  SRR4302537.2775950/94‑1 (MQ=60)
                                                                         AACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGG                      <  SRR4302537.6081084/94‑1 (MQ=60)
                                                                         AACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGG                      >  SRR4302537.1040105/1‑94 (MQ=60)
                                                                         AACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGG                      >  SRR4302537.652999/1‑94 (MQ=60)
                                                                         AACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGG                      <  SRR4302537.2503126/94‑1 (MQ=60)
                                                                          ACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGG                     <  SRR4302537.1531959/94‑1 (MQ=60)
                                                                          ACGTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGG                     <  SRR4302537.1260785/94‑1 (MQ=60)
                                                                            GTTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTC                   >  SRR4302537.452475/1‑94 (MQ=60)
                                                                             TTGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCA                  <  SRR4302537.3757310/94‑1 (MQ=60)
                                                                              TGCAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGGGGAGTCCAGCGGGGCAA                 >  SRR4302537.692734/1‑94 (MQ=60)
                                                                                CAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACG               >  SRR4302537.985918/1‑94 (MQ=60)
                                                                                CAGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACG               <  SRR4302537.4183810/94‑1 (MQ=60)
                                                                                 AGGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGGCAACGG              >  SRR4302537.1624449/1‑94 (MQ=60)
                                                                                  GGATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGC             >  SRR4302537.261210/1‑94 (MQ=60)
                                                                                   GATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACTGCTGGTGGAGTCCAGCGGGTCAACGGCC            >  SRR4302537.6306303/1‑94 (MQ=60)
                                                                                   GATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCC            <  SRR4302537.6198283/94‑1 (MQ=60)
                                                                                   GATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGGCCAGCGGGTCAACGGCC            >  SRR4302537.2373528/1‑94 (MQ=60)
                                                                                    ATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCG           <  SRR4302537.1860137/94‑1 (MQ=60)
                                                                                    ATGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCG           >  SRR4302537.1703933/1‑94 (MQ=60)
                                                                                    ACGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCG           <  SRR4302537.3688268/94‑1 (MQ=60)
                                                                                     TGGACTGATCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGG          >  SRR4302537.6374060/1‑94 (MQ=60)
                                                                                     TGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGG          <  SRR4302537.5875040/94‑1 (MQ=60)
                                                                                     TGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGG          <  SRR4302537.4256519/94‑1 (MQ=60)
                                                                                     TGGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGGCAACGGCCGG          >  SRR4302537.3951014/1‑94 (MQ=60)
                                                                                      GGACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGG         <  SRR4302537.4535246/94‑1 (MQ=60)
                                                                                       GACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGT        <  SRR4302537.421301/94‑1 (MQ=60)
                                                                                       GACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGT        <  SRR4302537.4480886/94‑1 (MQ=60)
                                                                                        ACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCCACGGCCGGGTG       >  SRR4302537.2323634/1‑94 (MQ=60)
                                                                                        ACTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTG       >  SRR4302537.3816156/1‑94 (MQ=60)
                                                                                        ACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTA       <  SRR4302537.4933793/94‑1 (MQ=60)
                                                                                         CTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGGCGGGTAG      >  SRR4302537.534984/1‑94 (MQ=60)
                                                                                         CTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAG      <  SRR4302537.470675/94‑1 (MQ=60)
                                                                                         CTGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAG      >  SRR4302537.5608028/1‑94 (MQ=60)
                                                                                          TGAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGGAGA     >  SRR4302537.813857/1‑94 (MQ=60)
                                                                                           GAGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGAT    <  SRR4302537.4724076/94‑1 (MQ=60)
                                                                                            AGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGGAGATA   >  SRR4302537.1309347/1‑94 (MQ=60)
                                                                                            AGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGGAGATA   >  SRR4302537.6436966/1‑94 (MQ=60)
                                                                                            AGCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGGGGAGGGCAGCGGGTAAACGGCgggggaagta   >  SRR4302537.5405653/1‑84 (MQ=60)
                                                                                             GCGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATAC  <  SRR4302537.5736488/94‑1 (MQ=60)
                                                                                             ACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATAC  <  SRR4302537.3886660/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATAC  >  NC_000913/3913829‑3914015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑