Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,937,396 T→C V281V (GTA→GTG)  yieO ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,937,3960TC96.9% 821.5 / 9.3 254V281V (GTA→GTG) yieOputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (2/6);  new base C (125/121);  total (127/127)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 267 total aligned reads displayed.

GAACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGG  >  NC_000913/3937307‑3937486
                                                                                         |                                                                                          
 aaCCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCacac                                                                                        <  1:6254501/93‑1 (MQ=255)
  aCCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGcacaca                                                                                       >  2:5446856/1‑93 (MQ=255)
  aCCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGcacaca                                                                                       <  1:4849482/93‑1 (MQ=255)
    cTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGc                                                                                     >  2:3901834/1‑93 (MQ=255)
    cTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGc                                                                                     >  1:4792276/1‑93 (MQ=255)
     tatcGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGcc                                                                                    <  2:531402/90‑1 (MQ=255)
         gCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTc                                                                                <  1:5549871/93‑1 (MQ=255)
         gCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTc                                                                                >  2:5360706/1‑93 (MQ=255)
         gCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTc                                                                                >  1:2161837/1‑93 (MQ=255)
          cTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTcc                                                                               >  1:225777/1‑93 (MQ=255)
           tGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTccc                                                                              >  1:910525/1‑93 (MQ=255)
            gTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTcccc                                                                             >  2:3715099/1‑93 (MQ=255)
            gTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTcccc                                                                             >  2:5924446/1‑93 (MQ=255)
              cGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAg                                                                           >  1:4165054/1‑93 (MQ=255)
                gTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGAc                                                                         >  2:3342472/1‑93 (MQ=255)
                 tGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTCCCCAGACg                                                                        <  1:4911811/93‑1 (MQ=255)
                 tGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACg                                                                        <  1:6313285/93‑1 (MQ=255)
                 tGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACg                                                                        >  1:3718600/1‑93 (MQ=255)
                  gCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACgg                                                                       <  2:6049912/93‑1 (MQ=255)
                   ccATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACggg                                                                      >  1:6177912/1‑93 (MQ=255)
                   ccATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACggg                                                                      <  2:878223/93‑1 (MQ=255)
                      tcatACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGGTg                                                                   >  2:3626720/1‑93 (MQ=255)
                       catACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGc                                                                  <  2:5267419/93‑1 (MQ=255)
                         tccagccagcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCaa                                                                <  1:5111167/91‑1 (MQ=255)
                         tACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCaa                                                                >  2:5115650/1‑93 (MQ=255)
                          aCAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAat                                                               <  1:5402743/93‑1 (MQ=255)
                           cagccagcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAata                                                              <  2:3216087/93‑1 (MQ=255)
                            agccagcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAatat                                                             >  1:3515933/1‑93 (MQ=255)
                            acccagcCATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAatat                                                             <  2:3109090/91‑1 (MQ=255)
                              ccagcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTg                                                           <  2:6112790/93‑1 (MQ=255)
                              ccagcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTg                                                           >  2:6195518/1‑93 (MQ=255)
                               cagcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGc                                                          >  1:4053381/1‑93 (MQ=255)
                                agcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGcc                                                         <  1:2547047/93‑1 (MQ=255)
                                agcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGCGTTGCAATATTGcc                                                         <  2:3943579/93‑1 (MQ=255)
                                agcAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAACGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGcc                                                         <  1:4553287/93‑1 (MQ=255)
                                 gcAATAAACGCCTGATACCCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCt                                                        <  1:5671954/93‑1 (MQ=255)
                                  cAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTa                                                       <  2:4453076/93‑1 (MQ=255)
                                   aaTAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAc                                                      >  2:2796555/1‑93 (MQ=255)
                                   aaTAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAc                                                      <  1:1055877/93‑1 (MQ=255)
                                   aaTAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAc                                                      >  1:4310725/1‑93 (MQ=255)
                                   aaTAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAc                                                      >  1:4702215/1‑93 (MQ=255)
                                   aaTAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAc                                                      <  1:2741908/93‑1 (MQ=255)
                                    aTAAACGCCTGATACCCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACg                                                     <  1:2564209/93‑1 (MQ=255)
                                    aTAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTACCCAGACGGGTTGCAATAATGCCTACg                                                     >  2:3295889/1‑93 (MQ=255)
                                     tAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGa                                                    <  1:5252224/93‑1 (MQ=255)
                                     aaaaCGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGa                                                    <  1:3067550/92‑1 (MQ=255)
                                       aaCGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGCTACCCAGACGGGTTGCAATATTGCCTACGATa                                                  >  1:5273456/1‑93 (MQ=255)
                                         cGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATAcc                                                >  1:5618924/1‑93 (MQ=255)
                                           ccTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGa                                              <  2:1927476/93‑1 (MQ=255)
                                            cTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGAt                                             >  1:2008422/1‑93 (MQ=255)
                                            cTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGAt                                             >  2:298164/1‑93 (MQ=255)
                                             tGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATc                                            >  1:6443422/1‑93 (MQ=255)
                                              gATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCg                                           >  2:5627216/1‑93 (MQ=255)
                                               aTAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCga                                          <  1:483877/93‑1 (MQ=255)
                                               aTAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCga                                          <  2:141304/93‑1 (MQ=255)
                                                 accaaaaTCCTCCCTGTAACATCAATGGCATAAGCAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCgaga                                        <  2:2357565/89‑1 (MQ=255)
                                                 aaCCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCgaga                                        >  1:2834485/1‑93 (MQ=255)
                                                 aaCAAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCgaga                                        <  2:4726324/93‑1 (MQ=255)
                                                  aCCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGaa                                       >  1:5051602/1‑93 (MQ=255)
                                                   ccAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGaaa                                      <  1:5945226/93‑1 (MQ=255)
                                                     aaaTCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGt                                    <  1:3798415/93‑1 (MQ=255)
                                                     aaaTCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGt                                    >  1:1005980/1‑93 (MQ=255)
                                                      aaTCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTg                                   >  2:5693605/1‑93 (MQ=255)
                                                       aTCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGc                                  >  1:4654209/1‑93 (MQ=255)
                                                        tcctccCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCg                                 <  2:1220994/93‑1 (MQ=255)
                                                        tCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCg                                 <  1:1415821/93‑1 (MQ=255)
                                                        tCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCg                                 >  2:2598719/1‑93 (MQ=255)
                                                          cTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCggg                               >  1:5773615/1‑93 (MQ=255)
                                                          cTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCggg                               >  2:5514219/1‑93 (MQ=255)
                                                          cTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCggg                               <  2:498762/93‑1 (MQ=255)
                                                           tACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGt                              >  2:5786975/1‑93 (MQ=255)
                                                            aCCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGtt                             <  1:5708628/93‑1 (MQ=255)
                                                            aCCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGtt                             <  1:715557/93‑1 (MQ=255)
                                                            aCCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGtt                             >  1:4383837/1‑93 (MQ=255)
                                                             ccTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGttt                            >  2:690950/1‑93 (MQ=255)
                                                             ccTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGttt                            >  1:3008622/1‑93 (MQ=255)
                                                                gTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTtaa                         >  2:756237/1‑93 (MQ=255)
                                                                gTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTtaa                         >  1:830506/1‑93 (MQ=255)
                                                                  aaCATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTtaaat                       <  2:4163424/93‑1 (MQ=255)
                                                                   aCATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTtaaata                      <  2:2532376/93‑1 (MQ=255)
                                                                   aCATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTtaaata                      >  2:3542584/1‑93 (MQ=255)
                                                                   aCATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTtaaata                      >  1:262003/1‑93 (MQ=255)
                                                                      tCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTaaataaat                   <  2:3993640/93‑1 (MQ=255)
                                                                        aaTGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCt                 <  1:1991510/93‑1 (MQ=255)
                                                                         aTGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTa                <  1:6394006/93‑1 (MQ=255)
                                                                         aTGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTa                <  2:2059599/93‑1 (MQ=255)
                                                                         aTGGCAAAAGAAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTa                <  2:4663980/93‑1 (MQ=255)
                                                                             cATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGa            >  1:1886218/1‑93 (MQ=255)
                                                                              aTAAGGAACGGTACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGaa           <  2:3431785/93‑1 (MQ=255)
                                                                              aTAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTTCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGaa           <  1:1826133/93‑1 (MQ=255)
                                                                              aTAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGaa           <  2:4809286/93‑1 (MQ=255)
                                                                               tAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGaaa          >  2:2150227/1‑93 (MQ=255)
                                                                                aaGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAAt         <  2:2721910/93‑1 (MQ=255)
                                                                                 aGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAAtt        <  1:1744118/93‑1 (MQ=255)
                                                                                   gAACGCCACACAGCCGTTCCCCAGCCGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTaa      <  2:6346794/93‑1 (MQ=255)
                                                                                   gAACGCCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTaa      <  2:4507988/93‑1 (MQ=255)
                                                                                    aaCGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAAt     <  1:3286795/93‑1 (MQ=255)
                                                                                    aaCGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAAt     >  2:5522594/1‑93 (MQ=255)
                                                                                     aCGGTACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATg    <  1:5123537/93‑1 (MQ=255)
                                                                                     aCGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATg    <  1:4331436/93‑1 (MQ=255)
                                                                                         |                                                                                          
GAACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGG  >  NC_000913/3937307‑3937486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGGAACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTT  >  NC_000913/3937303‑3937489
                                                                                             |                                                                                             
AATGGAACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGC                                                                                               <  SRR4302537.9833/94‑1 (MQ=60)
 ATGGAACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCA                                                                                              >  SRR4302537.488343/1‑94 (MQ=60)
   GGAACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACA                                                                                            >  SRR4302537.3060240/1‑94 (MQ=60)
     AACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACA                                                                                          <  SRR4302537.6297466/94‑1 (MQ=60)
      ACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAG                                                                                         <  SRR4302537.4882945/94‑1 (MQ=60)
      ACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAG                                                                                         >  SRR4302537.1802253/1‑94 (MQ=60)
       CCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGC                                                                                        <  SRR4302537.846843/94‑1 (MQ=60)
       CCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGC                                                                                        >  SRR4302537.6396951/1‑94 (MQ=60)
       CCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGC                                                                                        >  SRR4302537.4825352/1‑94 (MQ=60)
          AACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGG                                                                                     <  SRR4302537.5304166/94‑1 (MQ=60)
            CGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTC                                                                                   >  SRR4302537.1969098/1‑94 (MQ=60)
            CGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTC                                                                                   >  SRR4302537.2177074/1‑94 (MQ=60)
             GCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTCC                                                                                  >  SRR4302537.227379/1‑94 (MQ=60)
             GCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCC                                                                                  <  SRR4302537.5587766/94‑1 (MQ=60)
              CTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCC                                                                                 >  SRR4302537.916811/1‑94 (MQ=60)
                GTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCA                                                                               >  SRR4302537.2145382/1‑94 (MQ=60)
                 TCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAG                                                                              >  SRR4302537.4194144/1‑94 (MQ=60)
                 GCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAG                                                                              <  SRR4302537.569610/94‑1 (MQ=60)
                   GGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTCCCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGAC                                                                            <  SRR4302537.4882579/94‑1 (MQ=60)
                    GTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACG                                                                           >  SRR4302537.3744733/1‑94 (MQ=60)
                     TGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTCCCCAGACGG                                                                          <  SRR4302537.4945713/94‑1 (MQ=60)
                     TGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGG                                                                          <  SRR4302537.6356498/94‑1 (MQ=60)
                      TCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGG                                                                         >  SRR4302537.6220462/1‑94 (MQ=60)
                        CATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTT                                                                       >  SRR4302537.1891192/1‑94 (MQ=60)
                         ATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTG                                                                      <  SRR4302537.1861955/94‑1 (MQ=60)
                         ATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTG                                                                      >  SRR4302537.5150921/1‑94 (MQ=60)
                         ATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTG                                                                      >  SRR4302537.5150920/1‑94 (MQ=60)
                          TCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGC                                                                     >  SRR4302537.657006/1‑94 (MQ=60)
                           CATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCA                                                                    >  SRR4302537.4159605/1‑94 (MQ=60)
                             TCCAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAAT                                                                  <  SRR4302537.5146388/94‑1 (MQ=60)
                              ACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATA                                                                 <  SRR4302537.5439610/94‑1 (MQ=60)
                              ACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATA                                                                 >  SRR4302537.4313526/1‑94 (MQ=60)
                              ACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATA                                                                 <  SRR4302537.585920/94‑1 (MQ=60)
                               CAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATAT                                                                <  SRR4302537.5511113/94‑1 (MQ=60)
                               CAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATAT                                                                >  SRR4302537.3540808/1‑94 (MQ=60)
                                AGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATT                                                               >  SRR4302537.4524470/1‑94 (MQ=60)
                                 GCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTG                                                              >  SRR4302537.4374913/1‑94 (MQ=60)
                                  CCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGC                                                             >  SRR4302537.4081722/1‑94 (MQ=60)
                                   CAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCC                                                            >  SRR4302537.4343817/1‑94 (MQ=60)
                                    AGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCT                                                           <  SRR4302537.2564890/94‑1 (MQ=60)
                                    AGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCT                                                           <  SRR4302537.5320784/94‑1 (MQ=60)
                                    AGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAACGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCT                                                           <  SRR4302537.4584794/94‑1 (MQ=60)
                                     GCAATAAACGCCTGATACCCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTA                                                          <  SRR4302537.5710764/94‑1 (MQ=60)
                                     GCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGTCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTA                                                          <  SRR4302537.4779077/94‑1 (MQ=60)
                                      CAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAC                                                         >  SRR4302537.4734745/1‑94 (MQ=60)
                                      CAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAC                                                         <  SRR4302537.2576111/94‑1 (MQ=60)
                                      CAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTAC                                                         >  SRR4302537.4340683/1‑94 (MQ=60)
                                       AATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACG                                                        <  SRR4302537.1063161/94‑1 (MQ=60)
                                       AATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACG                                                        <  SRR4302537.2761037/94‑1 (MQ=60)
                                        ATAAACGCCTGATACCCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGA                                                       <  SRR4302537.2582173/94‑1 (MQ=60)
                                        ATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGA                                                       >  SRR4302537.1024640/1‑94 (MQ=60)
                                        ATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGA                                                       >  SRR4302537.55199/1‑94 (MQ=60)
                                         TAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGAT                                                      <  SRR4302537.5288364/94‑1 (MQ=60)
                                         AAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGAT                                                      <  SRR4302537.3088920/94‑1 (MQ=60)
                                          AAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATA                                                     >  SRR4302537.5004885/1‑94 (MQ=60)
                                          AAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATA                                                     >  SRR4302537.5830850/1‑94 (MQ=60)
                                          AAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGCTACCCAGACGGGTTGCAATATTGCCTACGATA                                                     >  SRR4302537.5309746/1‑94 (MQ=60)
                                           AACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATAC                                                    >  SRR4302537.6303729/1‑94 (MQ=60)
                                           AACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATAC                                                    >  SRR4302537.134741/1‑94 (MQ=60)
                                            ACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACC                                                   >  SRR4302537.5657348/1‑94 (MQ=60)
                                               CCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGAT                                                >  SRR4302537.2860297/1‑94 (MQ=60)
                                               CCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGAT                                                >  SRR4302537.2022420/1‑94 (MQ=60)
                                               CCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGAT                                                >  SRR4302537.6114264/1‑94 (MQ=60)
                                                CTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATC                                               <  SRR4302537.3296906/94‑1 (MQ=60)
                                                CTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATC                                               >  SRR4302537.4851811/1‑94 (MQ=60)
                                                CTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATC                                               >  SRR4302537.6487142/1‑94 (MQ=60)
                                                   ATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAG                                            >  SRR4302537.3861134/1‑94 (MQ=60)
                                                   ATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAG                                            <  SRR4302537.487192/94‑1 (MQ=60)
                                                    TAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGA                                           >  SRR4302537.2854306/1‑94 (MQ=60)
                                                     AACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAA                                          >  SRR4302537.5086384/1‑94 (MQ=60)
                                                     AACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAA                                          <  SRR4302537.4870906/94‑1 (MQ=60)
                                                     AACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAA                                          >  SRR4302537.568175/1‑94 (MQ=60)
                                                     AACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAA                                          <  SRR4302537.6201272/94‑1 (MQ=60)
                                                     AACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAA                                          <  SRR4302537.4798625/94‑1 (MQ=60)
                                                      ACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAA                                         <  SRR4302537.4913912/94‑1 (MQ=60)
                                                       CCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAG                                        <  SRR4302537.5986033/94‑1 (MQ=60)
                                                        CAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGT                                       >  SRR4302537.4449172/1‑94 (MQ=60)
                                                        CAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGT                                       <  SRR4302537.1985414/94‑1 (MQ=60)
                                                        CAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGT                                       >  SRR4302537.1012924/1‑94 (MQ=60)
                                                        CAAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGT                                       <  SRR4302537.3432058/94‑1 (MQ=60)
                                                         AAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTG                                      <  SRR4302537.2908109/94‑1 (MQ=60)
                                                         AAATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTG                                      <  SRR4302537.3825063/94‑1 (MQ=60)
                                                          AATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGC                                     >  SRR4302537.4686394/1‑94 (MQ=60)
                                                           ATCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCG                                    <  SRR4302537.2600914/94‑1 (MQ=60)
                                                            TCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGG                                   <  SRR4302537.5311091/94‑1 (MQ=60)
                                                            TCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGG                                   <  SRR4302537.4589912/94‑1 (MQ=60)
                                                            TCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGG                                   >  SRR4302537.423824/1‑94 (MQ=60)
                                                            TCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGG                                   <  SRR4302537.2807520/94‑1 (MQ=60)
                                                            TCCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGG                                   <  SRR4302537.1425675/94‑1 (MQ=60)
                                                             CCTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGG                                  >  SRR4302537.5813189/1‑94 (MQ=60)
                                                              CTACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGT                                 <  SRR4302537.5197239/94‑1 (MQ=60)
                                                               TACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTT                                >  SRR4302537.4414089/1‑94 (MQ=60)
                                                               TACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTT                                <  SRR4302537.1095716/94‑1 (MQ=60)
                                                                ACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTT                               <  SRR4302537.6303112/94‑1 (MQ=60)
                                                                ACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTT                               <  SRR4302537.5747693/94‑1 (MQ=60)
                                                                ACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTT                               <  SRR4302537.720447/94‑1 (MQ=60)
                                                                ACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTT                               >  SRR4302537.818133/1‑94 (MQ=60)
                                                                ACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTT                               >  SRR4302537.3029563/1‑94 (MQ=60)
                                                                ACCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTT                               <  SRR4302537.4883114/94‑1 (MQ=60)
                                                                 CCTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTT                              >  SRR4302537.4670828/1‑94 (MQ=60)
                                                                  CTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTA                             <  SRR4302537.6458855/94‑1 (MQ=60)
                                                                  CTGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTA                             >  SRR4302537.5759726/1‑94 (MQ=60)
                                                                   TGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAA                            >  SRR4302537.836187/1‑94 (MQ=60)
                                                                   TGTAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAA                            <  SRR4302537.1666025/94‑1 (MQ=60)
                                                                     TAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAAT                          >  SRR4302537.5728896/1‑94 (MQ=60)
                                                                     TAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAAT                          >  SRR4302537.366799/1‑94 (MQ=60)
                                                                     TAACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAAT                          <  SRR4302537.4348000/94‑1 (MQ=60)
                                                                      AACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATA                         >  SRR4302537.263850/1‑94 (MQ=60)
                                                                      AACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATA                         <  SRR4302537.2088166/94‑1 (MQ=60)
                                                                      AACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATA                         <  SRR4302537.2588844/94‑1 (MQ=60)
                                                                       ACATCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAA                        <  SRR4302537.6391911/94‑1 (MQ=60)
                                                                          TCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATC                     >  SRR4302537.2247155/1‑94 (MQ=60)
                                                                          TCAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATC                     >  SRR4302537.3798673/1‑94 (MQ=60)
                                                                           CAATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCT                    <  SRR4302537.331465/94‑1 (MQ=60)
                                                                            AATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTA                   <  SRR4302537.2005348/94‑1 (MQ=60)
                                                                             ATGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAA                  <  SRR4302537.6437555/94‑1 (MQ=60)
                                                                              TGGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAAT                 <  SRR4302537.2528732/94‑1 (MQ=60)
                                                                               GGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATG                <  SRR4302537.5741698/94‑1 (MQ=60)
                                                                               GGCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATG                <  SRR4302537.3177466/94‑1 (MQ=60)
                                                                                GCATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGA               >  SRR4302537.1899336/1‑94 (MQ=60)
                                                                                 CATCAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCATACGATACCGATCGAGAAAGTGCGGGTTTTAAATAACTCTAATGAG              <  SRR4302537.6514502/94‑1 (MQ=60)
                                                                                  ATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTTCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAA             <  SRR4302537.1838860/94‑1 (MQ=60)
                                                                                  ATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAA             >  SRR4302537.201135/1‑94 (MQ=60)
                                                                                  ATAAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGGTTTAAATAAATCTAATGAAA             >  SRR4302537.5096401/1‑94 (MQ=60)
                                                                                    AGGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATT           <  SRR4302537.743871/94‑1 (MQ=60)
                                                                                    AAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATT           >  SRR4302537.285061/1‑94 (MQ=60)
                                                                                    AAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATT           <  SRR4302537.2219915/94‑1 (MQ=60)
                                                                                    AAGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATT           <  SRR4302537.1583862/94‑1 (MQ=60)
                                                                                     AGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTA          <  SRR4302537.2566928/94‑1 (MQ=60)
                                                                                     AGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTA          <  SRR4302537.1756278/94‑1 (MQ=60)
                                                                                     AGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTA          >  SRR4302537.4713199/1‑94 (MQ=60)
                                                                                     AGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTA          >  SRR4302537.374683/1‑94 (MQ=60)
                                                                                     AGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTA          <  SRR4302537.4946270/94‑1 (MQ=60)
                                                                                     AGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTA          <  SRR4302537.5189087/94‑1 (MQ=60)
                                                                                     AGGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATAA          >  SRR4302537.5597390/1‑94 (MQ=60)
                                                                                      GGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAA         <  SRR4302537.6294304/94‑1 (MQ=60)
                                                                                      GGAACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAA         >  SRR4302537.2723582/1‑94 (MQ=60)
                                                                                        AACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATG       <  SRR4302537.3310021/94‑1 (MQ=60)
                                                                                        AACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGGTTTAAATAAATCTAATGAAATTAATG       >  SRR4302537.4067371/1‑94 (MQ=60)
                                                                                         ACGGTACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGG      <  SRR4302537.5158848/94‑1 (MQ=60)
                                                                                         ACGGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGG      <  SRR4302537.4361524/94‑1 (MQ=60)
                                                                                           GGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGT    >  SRR4302537.3330956/1‑94 (MQ=60)
                                                                                           GGCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGT    <  SRR4302537.394549/94‑1 (MQ=60)
                                                                                            GCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTT   >  SRR4302537.2550825/1‑94 (MQ=60)
                                                                                            GCACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTT   <  SRR4302537.5397413/94‑1 (MQ=60)
                                                                                             CACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTT  <  SRR4302537.6217048/94‑1 (MQ=60)
                                                                                             CACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTT  >  SRR4302537.1050155/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATGGAACCTAACGCTGTCGGTGCCATCATACAGCCAGCAATAAACGCCTGATAACCAAATCCTACCTGTAACATCAATGGCATAAGGAACGGTACACAGCCGGTCCCCAGACGGGTTGCAATATTGCCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTT  >  NC_000913/3937303‑3937489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑