Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,938,138	A→G	L34S (TTA→TCA)	yieO ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,938,138	0	A	G	99.6%	776.3 / ‑4.9	229	L34S (TTA→TCA)	yieO	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (139/89); minor base T (0/1); total (139/90)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

Only 100 of 243 total aligned reads displayed.

GGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTT  >  NC_000913/3938048‑3938227

ggAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAg                                                                                         >  2:2704895/1‑93 (MQ=255)

ggAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAg                                                                                         <  2:2192792/93‑1 (MQ=255)

ggAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAg                                                                                         >  1:2270676/1‑93 (MQ=255)

ggAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAg                                                                                         <  1:1796141/93‑1 (MQ=255)

 gAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGAGTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAgg                                                                                        >  2:5787283/1‑93 (MQ=255)

    tAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCgg                                                                                     >  2:4325538/1‑93 (MQ=255)

      aGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTa                                                                                   >  1:6014983/1‑93 (MQ=255)

       gCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTAt                                                                                  <  1:2634237/93‑1 (MQ=255)

        cATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTAtt                                                                                 <  1:2329476/93‑1 (MQ=255)

           cGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAg                                                                              <  2:4593074/93‑1 (MQ=255)

            gCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGa                                                                             >  1:1322139/1‑93 (MQ=255)

             ccACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGaa                                                                            <  1:789243/93‑1 (MQ=255)

              cACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAAt                                                                           >  2:2900745/1‑93 (MQ=255)

               aCCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGATGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATa                                                                          <  1:5380439/93‑1 (MQ=255)

               aCCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATa                                                                          <  2:1072980/93‑1 (MQ=255)

                 cGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGt                                                                        >  1:2215062/1‑93 (MQ=255)

                 cGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGt                                                                        <  1:1277199/93‑1 (MQ=255)

                  gTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTg                                                                       >  1:4203154/1‑93 (MQ=255)

                  gTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTg                                                                       <  2:2248727/93‑1 (MQ=255)

                  gTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGACGAGAACGATTAAGGCTATGATCGATTGCGGGTGAGGCGGTATTAAGAATAGTg                                                                       >  2:2390785/1‑93 (MQ=255)

                   tCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTgg                                                                      >  2:6245398/1‑93 (MQ=255)

                   tCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTgg                                                                      <  2:5517025/93‑1 (MQ=255)

                   tCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTgg                                                                      >  1:2513940/1‑93 (MQ=255)

                   tCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTgg                                                                      >  1:5694133/1‑93 (MQ=255)

                     aGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCa                                                                    >  1:3167208/1‑93 (MQ=255)

                     aGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCa                                                                    >  2:5014916/1‑93 (MQ=255)

                      gCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCAt                                                                   >  2:4674459/1‑93 (MQ=255)

                      gCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCAt                                                                   <  1:1581768/93‑1 (MQ=255)

                      gCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCAt                                                                   <  2:6049575/93‑1 (MQ=255)

                        gTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCa                                                                 <  1:4439899/93‑1 (MQ=255)

                        gTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCa                                                                 >  1:6257388/1‑93 (MQ=255)

                        gTATAACTGATGATGGCTGATTGCATCGCGAGAGCAGAACGATTAAGGCTATGAGCGATTGCGGGTGGGGCGGTATTAAGAATAGCAGCAtta                                                                 >  2:31582/1‑91 (MQ=255)

                         tataACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCaa                                                                >  1:2681629/1‑93 (MQ=255)

                         tataACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCaa                                                                >  1:382205/1‑93 (MQ=255)

                         tataACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCaa                                                                >  2:5303851/1‑93 (MQ=255)

                          ataACTGATGATGGCTGATTGCATCCCGGGAGGAAAAAGATTAAGGCTATGCGCGATTGCGGGTGAGGCGGAATTAAGAATAGTGGCATCAAg                                                               >  2:1911107/1‑93 (MQ=255)

                           taACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGt                                                              <  2:3071112/93‑1 (MQ=255)

                           taACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGt                                                              >  1:3588489/1‑93 (MQ=255)

                            aaCTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTg                                                             >  2:474536/1‑93 (MQ=255)

                            aaCTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTg                                                             >  1:2742753/1‑93 (MQ=255)

                              cTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGcc                                                           <  1:4564544/93‑1 (MQ=255)

                                gatgatGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTg                                                         >  2:3959291/1‑93 (MQ=255)

                                gatgatGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTg                                                         >  2:5704063/1‑93 (MQ=255)

                                 atgatgGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGCGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGc                                                        >  1:836499/1‑93 (MQ=255)

                                  tgatgGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCa                                                       >  1:3015032/1‑93 (MQ=255)

                                  tgatgGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCa                                                       <  1:3878077/93‑1 (MQ=255)

                                    atgGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATg                                                     >  1:4097209/1‑93 (MQ=255)

                                       gCTGATTGCATCGCGAGAGGCGAACGATTAAGGCTATGAGGGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGGCTGCCTgaag                                                  >  2:368195/1‑93 (MQ=255)

                                        cTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATgaaga                                                 <  2:3447495/93‑1 (MQ=255)

                                        cTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATgaaga                                                 <  1:3730338/93‑1 (MQ=255)

                                        cTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATgaaga                                                 <  1:3971307/93‑1 (MQ=255)

                                         tGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATgaagaa                                                <  1:2643429/93‑1 (MQ=255)

                                         tGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATgaagaa                                                <  1:5057917/93‑1 (MQ=255)

                                          gATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGaagaag                                               >  1:299437/1‑93 (MQ=255)

                                          gATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGaagaag                                               >  2:2611547/1‑93 (MQ=255)

                                           aTTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTACCTGCATGAAGAAgg                                              >  2:580956/1‑93 (MQ=255)

                                            ttGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGc                                             >  2:6432477/1‑93 (MQ=255)

                                            ttGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGc                                             >  2:3589619/1‑93 (MQ=255)

                                            ttGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGc                                             >  1:4884400/1‑93 (MQ=255)

                                             tGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGcc                                            >  2:2566172/1‑93 (MQ=255)

                                             tGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGcc                                            >  1:549198/1‑93 (MQ=255)

                                             tGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGcc                                            >  2:873337/1‑93 (MQ=255)

                                              gCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCa                                           >  2:4005331/1‑93 (MQ=255)

                                                 tCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATcg                                        >  2:3239614/1‑93 (MQ=255)

                                                   gcgAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATcgcc                                      <  2:421698/93‑1 (MQ=255)

                                                   gcgAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATcgcc                                      >  1:1198954/1‑93 (MQ=255)

                                                    cgAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATcgccg                                     <  1:5726137/93‑1 (MQ=255)

                                                    cgAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATcgccg                                     >  2:4074937/1‑93 (MQ=255)

                                                    cgAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGCGCCTGCATGAAGAAGGCAATggcgg                                     >  1:3234968/1‑91 (MQ=255)

                                                       gaggagAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGa                                  >  1:1017597/1‑93 (MQ=255)

                                                       gaggagAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGa                                  <  1:2585362/93‑1 (MQ=255)

                                                         ggagAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATc                                >  1:480210/1‑93 (MQ=255)

                                                          gagaACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATcc                               >  1:3716118/1‑93 (MQ=255)

                                                          gagaACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATcc                               >  1:5945339/1‑93 (MQ=255)

                                                           agaCCGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCa                              <  2:722128/93‑1 (MQ=255)

                                                            gaACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCAc                             >  2:1639830/1‑93 (MQ=255)

                                                            gaACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGGATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCAc                             >  2:1868831/1‑93 (MQ=255)

                                                             aaCGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACg                            <  2:4843535/93‑1 (MQ=255)

                                                              aCGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACgg                           >  2:4754506/1‑93 (MQ=255)

                                                               cGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACgga                          >  1:4414474/1‑92 (MQ=255)

                                                                 aTTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCaa                        >  1:583252/1‑93 (MQ=255)

                                                                 aTTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCaa                        >  2:4393090/1‑93 (MQ=255)

                                                                 aTTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCaa                        >  1:4994266/1‑93 (MQ=255)

                                                                   tAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAAc                      <  1:1729028/93‑1 (MQ=255)

                                                                      ggCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAAcccc                   >  1:5155181/1‑92 (MQ=255)

                                                                      ggCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCg                   >  1:976484/1‑93 (MQ=255)

                                                                      ggCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCg                   >  2:342739/1‑93 (MQ=255)

                                                                        cTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGcc                 >  1:3609571/1‑93 (MQ=255)

                                                                         tATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCa                >  2:1794519/1‑93 (MQ=255)

                                                                          aTGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCAt               >  1:3533689/1‑93 (MQ=255)

                                                                           tGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATa              >  1:466662/1‑93 (MQ=255)

                                                                           tGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATa              <  2:1361651/93‑1 (MQ=255)

                                                                            gAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATAc             <  2:893195/93‑1 (MQ=255)

                                                                                gATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTgcg         >  1:6232108/1‑93 (MQ=255)

                                                                                gATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTgcg         >  1:1987041/1‑93 (MQ=255)

                                                                                 aTTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTgcgc        >  2:1487139/1‑93 (MQ=255)

                                                                                  tggCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGct       <  2:2307873/91‑1 (MQ=255)

                                                                                      gggTGAGGCGTTATAAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAACCCCGCCATACTGCGcttctt   <  2:1693065/93‑1 (MQ=255)

                                                                                      gggTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGcttctt   >  2:2532170/1‑93 (MQ=255)

                                                                                       ggTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCttt  >  1:6430034/1‑93 (MQ=255)

GGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTT  >  NC_000913/3938048‑3938227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTAT  >  NC_000913/3938047‑3938231

CGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAG                                                                                             <  SRR4302537.6393049/94‑1 (MQ=60)

CGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAG                                                                                             >  SRR4302537.2286711/1‑94 (MQ=60)

CGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAG                                                                                             <  SRR4302537.2301148/94‑1 (MQ=60)

CGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAG                                                                                             >  SRR4302537.3752845/1‑94 (MQ=60)

 GGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGG                                                                                            <  SRR4302537.1808676/94‑1 (MQ=60)

  GAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGC                                                                                           >  SRR4302537.4184802/1‑94 (MQ=60)

     TAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGT                                                                                        >  SRR4302537.4737712/1‑94 (MQ=60)

      AAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTT                                                                                       >  SRR4302537.1320193/1‑94 (MQ=60)

      AAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTA                                                                                       >  SRR4302537.6056307/1‑94 (MQ=60)

       AGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTAT                                                                                      <  SRR4302537.5967790/94‑1 (MQ=60)

       AGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTAT                                                                                      >  SRR4302537.5053260/1‑94 (MQ=60)

        GCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATT                                                                                     <  SRR4302537.2652672/94‑1 (MQ=60)

         CATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTA                                                                                    <  SRR4302537.2345905/94‑1 (MQ=60)

         CATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTA                                                                                    >  SRR4302537.4447802/1‑94 (MQ=60)

          ATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAA                                                                                   >  SRR4302537.899549/1‑94 (MQ=60)

          ATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAA                                                                                   >  SRR4302537.583282/1‑94 (MQ=60)

           TCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAG                                                                                  <  SRR4302537.2617134/94‑1 (MQ=60)

           TCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAG                                                                                  <  SRR4302537.5526106/94‑1 (MQ=60)

            CGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGA                                                                                 >  SRR4302537.832638/1‑94 (MQ=60)

            CGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGA                                                                                 >  SRR4302537.1331353/1‑94 (MQ=60)

             GCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAA                                                                                <  SRR4302537.2653279/94‑1 (MQ=60)

             GCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAA                                                                                >  SRR4302537.6291786/1‑94 (MQ=60)

             GCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAA                                                                                <  SRR4302537.2658337/94‑1 (MQ=60)

             GCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAA                                                                                >  SRR4302537.3808340/1‑94 (MQ=60)

             GCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAA                                                                                <  SRR4302537.4492051/94‑1 (MQ=60)

             GCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAA                                                                                <  SRR4302537.2531246/94‑1 (MQ=60)

             GCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAAGCGGTATTAAGAA                                                                                >  SRR4302537.3717188/1‑94 (MQ=60)

              CCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAAT                                                                               <  SRR4302537.794662/94‑1 (MQ=60)

               CACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATA                                                                              >  SRR4302537.3624673/1‑94 (MQ=60)

                ACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGATGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAG                                                                             <  SRR4302537.5417233/94‑1 (MQ=60)

                ACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAG                                                                             >  SRR4302537.1246697/1‑94 (MQ=60)

                ACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAG                                                                             <  SRR4302537.1999331/94‑1 (MQ=60)

                 CCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGT                                                                            >  SRR4302537.4492227/1‑94 (MQ=60)

                 CCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGT                                                                            >  SRR4302537.2230683/1‑94 (MQ=60)

                  CGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTG                                                                           >  SRR4302537.4232497/1‑94 (MQ=60)

                  CGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTG                                                                           <  SRR4302537.6160153/94‑1 (MQ=60)

                  CGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTG                                                                           <  SRR4302537.3291536/94‑1 (MQ=60)

                  CGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTG                                                                           <  SRR4302537.1286074/94‑1 (MQ=60)

                   GTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGG                                                                          <  SRR4302537.20674/94‑1 (MQ=60)

                   GTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGG                                                                          >  SRR4302537.5733082/1‑94 (MQ=60)

                   GTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGG                                                                          >  SRR4302537.2531610/1‑94 (MQ=60)

                   GTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGG                                                                          >  SRR4302537.5950070/1‑94 (MQ=60)

                    TCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGC                                                                         <  SRR4302537.2328229/94‑1 (MQ=60)

                    TCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGC                                                                         <  SRR4302537.4647107/94‑1 (MQ=60)

                     CAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCA                                                                        <  SRR4302537.1094073/94‑1 (MQ=60)

                     CAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCA                                                                        >  SRR4302537.1637249/1‑94 (MQ=60)

                     CAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCA                                                                        <  SRR4302537.6319686/94‑1 (MQ=60)

                     CAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCA                                                                        >  SRR4302537.3189455/1‑94 (MQ=60)

                      AGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCAT                                                                       >  SRR4302537.1704915/1‑94 (MQ=60)

                      AGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCAT                                                                       <  SRR4302537.3723596/94‑1 (MQ=60)

                       GCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATC                                                                      <  SRR4302537.1592825/94‑1 (MQ=60)

                        CGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCA                                                                     >  SRR4302537.6300364/1‑94 (MQ=60)

                         GTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAA                                                                    >  SRR4302537.3896317/1‑94 (MQ=60)

                         GTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAA                                                                    >  SRR4302537.384852/1‑94 (MQ=60)

                         GTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAA                                                                    <  SRR4302537.4470555/94‑1 (MQ=60)

                         GTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAA                                                                    >  SRR4302537.2700352/1‑94 (MQ=60)

                          TATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAG                                                                   <  SRR4302537.5017307/94‑1 (MQ=60)

                          TATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAG                                                                   <  SRR4302537.4280441/94‑1 (MQ=60)

                           ATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGT                                                                  >  SRR4302537.3613786/1‑94 (MQ=60)

                            TAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTG                                                                 >  SRR4302537.2761888/1‑94 (MQ=60)

                               CTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCT                                                              <  SRR4302537.4596141/94‑1 (MQ=60)

                               CTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCT                                                              >  SRR4302537.3168763/1‑94 (MQ=60)

                                 GATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGC                                                            >  SRR4302537.660676/1‑94 (MQ=60)

                                 GATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGCGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGC                                                            >  SRR4302537.842225/1‑94 (MQ=60)

                                  ATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCA                                                           >  SRR4302537.2809169/1‑94 (MQ=60)

                                  ATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCA                                                           <  SRR4302537.5377110/94‑1 (MQ=60)

                                  ATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCA                                                           >  SRR4302537.3036052/1‑94 (MQ=60)

                                  ATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCA                                                           >  SRR4302537.5727669/1‑94 (MQ=60)

                                   TGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCAT                                                          >  SRR4302537.852945/1‑94 (MQ=60)

                                   TGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCAT                                                          <  SRR4302537.3905200/94‑1 (MQ=60)

                                    GATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATG                                                         >  SRR4302537.4125816/1‑94 (MQ=60)

                                       GGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAG                                                      >  SRR4302537.1989396/1‑94 (MQ=60)

                                        GCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGA                                                     >  SRR4302537.3404896/1‑94 (MQ=60)

                                         CTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAA                                                    <  SRR4302537.3756543/94‑1 (MQ=60)

                                         CTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAA                                                    <  SRR4302537.3999100/94‑1 (MQ=60)

                                          TGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAG                                                   <  SRR4302537.2661922/94‑1 (MQ=60)

                                          TGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAG                                                   <  SRR4302537.5092735/94‑1 (MQ=60)

                                          TGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAG                                                   <  SRR4302537.5128039/94‑1 (MQ=60)

                                          TGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAG                                                   <  SRR4302537.3952249/94‑1 (MQ=60)

                                          TGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAG                                                   >  SRR4302537.301525/1‑94 (MQ=60)

                                            ATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGC                                                 >  SRR4302537.4918131/1‑94 (MQ=60)

                                             TTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCC                                                >  SRR4302537.552981/1‑94 (MQ=60)

                                              TGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCA                                               >  SRR4302537.3200117/1‑94 (MQ=60)

                                               GCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCAT                                              <  SRR4302537.479765/94‑1 (MQ=60)

                                                 ATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCG                                            >  SRR4302537.5385044/1‑94 (MQ=60)

                                                 ATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCG                                            <  SRR4302537.2688823/94‑1 (MQ=60)

                                                 ATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCG                                            <  SRR4302537.3015683/94‑1 (MQ=60)

                                                 ATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCG                                            >  SRR4302537.3040025/1‑94 (MQ=60)

                                                   CGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCC                                          >  SRR4302537.1207286/1‑94 (MQ=60)

                                                    GCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGCGCCTGCATGAAGAAGGCaatggcgg                                         >  SRR4302537.3257771/1‑86 (MQ=60)

                                                     CGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGC                                        <  SRR4302537.5765335/94‑1 (MQ=60)

                                                      GAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCG                                       <  SRR4302537.4071902/94‑1 (MQ=60)

                                                      GAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCG                                       <  SRR4302537.1800948/94‑1 (MQ=60)

                                                      GAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCG                                       >  SRR4302537.1300148/1‑94 (MQ=60)

                                                       AGAGTAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGA                                      >  SRR4302537.3008938/1‑94 (MQ=60)

                                                       AGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGA                                      >  SRR4302537.1024602/1‑94 (MQ=60)

                                                        GAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGAT                                     <  SRR4302537.2603454/94‑1 (MQ=60)

                                                        GAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGAT                                     >  SRR4302537.2260407/1‑94 (MQ=60)

                                                         AGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATC                                    >  SRR4302537.483503/1‑94 (MQ=60)

                                                         AGGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATC                                    >  SRR4302537.6347858/1‑94 (MQ=60)

                                                          GGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCC                                   >  SRR4302537.5986146/1‑94 (MQ=60)

                                                          GGAGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCC                                   >  SRR4302537.3742238/1‑94 (MQ=60)

                                                           GAGAACGATTAAGGCTATGAGCGATTGCGGGTTAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCA                                  <  SRR4302537.3794882/94‑1 (MQ=60)

                                                            AGAACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCAC                                 <  SRR4302537.4330527/94‑1 (MQ=60)

                                                              AACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGG                               >  SRR4302537.2567051/1‑94 (MQ=60)

                                                               ACGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGA                              >  SRR4302537.4444945/1‑94 (MQ=60)

                                                                CGATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCA                             >  SRR4302537.6004623/1‑94 (MQ=60)

                                                                 GATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAA                            >  SRR4302537.587239/1‑94 (MQ=60)

                                                                 GATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAA                            >  SRR4302537.5028679/1‑94 (MQ=60)

                                                                 GATTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAA                            >  SRR4302537.2314942/1‑94 (MQ=60)

                                                                  ATTAAGGCTATGAGCGATTGCGGGGGAGGCGGTATTAAGAATAGTGGCATCACGTGCCTGCATGAAGAAGGCCCTCGCCGCGATCCACGGCAAA                           >  SRR4302537.2704301/1‑94 (MQ=60)

                                                                   TTAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAAC                          >  SRR4302537.4903560/1‑94 (MQ=60)

                                                                    TAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACC                         <  SRR4302537.5740672/94‑1 (MQ=60)

                                                                    TAAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACC                         <  SRR4302537.1741100/94‑1 (MQ=60)

                                                                     AAGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCC                        <  SRR4302537.3589555/94‑1 (MQ=60)

                                                                      AGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCG                       >  SRR4302537.983217/1‑94 (MQ=60)

                                                                      AGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCG                       <  SRR4302537.2565528/94‑1 (MQ=60)

                                                                      AGGCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCC                       >  SRR4302537.5190701/1‑94 (MQ=60)

                                                                        GCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCC                     >  SRR4302537.3635016/1‑94 (MQ=60)

                                                                        GCTATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCC                     >  SRR4302537.625197/1‑94 (MQ=60)

                                                                          TATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCAT                   >  SRR4302537.1481933/1‑94 (MQ=60)

                                                                          TATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCAT                   >  SRR4302537.3558658/1‑94 (MQ=60)

                                                                           ATGAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATA                  >  SRR4302537.469869/1‑94 (MQ=60)

                                                                             GAGCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGCCCATCGCCGCGATCCACGGCAAACCCGCCATACT                <  SRR4302537.3946833/94‑1 (MQ=60)

                                                                               GCGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGCCCATCGCCGCGACCCACGGCAAACCCGCCATACTGC              <  SRR4302537.2849612/94‑1 (MQ=60)

                                                                                CGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCG             >  SRR4302537.6274974/1‑94 (MQ=60)

                                                                                CGATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCG             >  SRR4302537.2000857/1‑94 (MQ=60)

                                                                                 GATTGCGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGC            >  SRR4302537.5707444/1‑94 (MQ=60)

                                                                                      GGGGTGAGGCGTTATTAAGAATAGTGGCATCAGGTGCCTGCATGAAGAAGGCCACCGCCGCAATCCACGGCAACCCCGCCATACTGCGCTTCTT       <  SRR4302537.4555762/94‑1 (MQ=60)

                                                                                      GGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCATGCAGGAAGAAGGCCATCGCCGCGACCCACGGCAAACCCGCCATACTGCGATTCTT       <  SRR4302537.3350060/94‑1 (MQ=60)

                                                                                      CGGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTT       >  SRR4302537.5088852/1‑94 (MQ=60)

                                                                                       GGGTGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTT      >  SRR4302537.6473717/1‑94 (MQ=60)

                                                                                         GTGAGGCGGTATAAAGAATAGTGGCATCAGGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAACCCCGCCATACTGCGCTTCTTTTT    <  SRR4302537.502770/94‑1 (MQ=60)

                                                                                          TGAGGGGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAACCCCGCCATACTGCGCTTCTTTTTA   <  SRR4302537.1934557/94‑1 (MQ=60)

                                                                                          TGAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTA   >  SRR4302537.1064967/1‑94 (MQ=60)

                                                                                           GAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTAT  >  SRR4302537.4178191/1‑94 (MQ=60)

                                                                                           GAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGCCCATCGCCGCGATCCACGGCAACCCCGCCATACTGCGCTTCTTTTTAT  <  SRR4302537.1324783/94‑1 (MQ=60)

CGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTAT  >  NC_000913/3938047‑3938231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`