Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,945,351	+C	coding (1/600 nt) coding (240/240 nt)	yifDA ← yifDA ←	transcription regulator transcription regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,945,350	1	.	C	100.0%	786.5 / NA	223	E81G (GAG→GGG)	yifDA	transcription regulator
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (121/102); total (121/102)

Only 100 of 240 total aligned reads displayed.

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

aCAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct                                                                                          >  2:5193375/1‑93 (MQ=255)

aCAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct                                                                                          >  2:2401220/1‑93 (MQ=255)

aCAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct                                                                                          >  1:4431742/1‑93 (MQ=255)

 cAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑c                                                                                        >  2:3609765/1‑93 (MQ=255)

  aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cc                                                                                       <  1:6476287/93‑2 (MQ=255)

   aGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cct                                                                                      <  2:6263068/93‑3 (MQ=255)

   aGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cct                                                                                      <  2:4579686/93‑3 (MQ=255)

       tCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACg                                                                                   <  1:1069070/93‑1 (MQ=255)

       ccccAGCCACTGATTAACCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACg                                                                                   <  2:2664361/92‑1 (MQ=255)

        cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACgg                                                                                  >  2:1592953/1‑93 (MQ=255)

         ccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACggg                                                                                 >  1:4233072/1‑93 (MQ=255)

                cTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTg                                                                          <  1:3358290/93‑1 (MQ=255)

                cTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTg                                                                          >  1:2382512/1‑93 (MQ=255)

                 tGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGAGAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGc                                                                         >  2:5265545/1‑93 (MQ=255)

                   aTTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCa                                                                       >  2:1385135/1‑93 (MQ=255)

                   aTTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCa                                                                       >  1:1587551/1‑93 (MQ=255)

                     tAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAgg                                                                     <  1:3630702/93‑1 (MQ=255)

                      aaGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGt                                                                    <  2:1783190/93‑1 (MQ=255)

                      aaGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGt                                                                    <  1:4343279/93‑1 (MQ=255)

                        gCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGc                                                                  >  1:4901724/1‑93 (MQ=255)

                        gCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGc                                                                  <  1:4699958/93‑1 (MQ=255)

                         cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCt                                                                 >  1:2759421/1‑93 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               >  1:3717532/1‑93 (MQ=255)

                              aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            >  1:2536556/1‑93 (MQ=255)

                                 cccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCg                                                         >  2:2208952/1‑93 (MQ=255)

                                  ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  2:1649209/1‑93 (MQ=255)

                                   cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       <  2:5695052/93‑1 (MQ=255)

                                   cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       >  1:5252011/1‑93 (MQ=255)

                                    acaACGAGGCGTTGACACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt                                                      <  1:5798505/93‑1 (MQ=255)

                                    acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt                                                      <  2:4270418/93‑1 (MQ=255)

                                    acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGCTGCTCATGAGCGttt                                                      >  2:5413463/1‑93 (MQ=255)

                                    acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCCCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt                                                      >  2:4838934/1‑93 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  2:1984664/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  1:1719227/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  1:1262334/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  2:736915/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGACAGGTGCTCATGAGCGttta                                                     >  1:2929879/1‑92 (MQ=255)

                                        cGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc                                                  <  1:1533700/93‑1 (MQ=255)

                                        cGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc                                                  <  2:2746766/93‑1 (MQ=255)

                                         gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCa                                                 >  2:5620428/1‑93 (MQ=255)

                                          aGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAt                                                >  2:211921/1‑93 (MQ=255)

                                          aGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAt                                                >  2:4448655/1‑93 (MQ=255)

                                          aGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAt                                                >  1:4053748/1‑93 (MQ=255)

                                              gcTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAgg                                            >  1:5206949/1‑93 (MQ=255)

                                               cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGc                                           <  1:2287848/93‑1 (MQ=255)

                                               cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGc                                           <  2:4195360/93‑1 (MQ=255)

                                                tGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa                                          >  1:5759145/1‑93 (MQ=255)

                                                tGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa                                          <  1:3099636/93‑1 (MQ=255)

                                                 ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCag                                         >  1:2130107/1‑93 (MQ=255)

                                                  gCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagt                                        >  1:1904916/1‑93 (MQ=255)

                                                   cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       >  1:3312150/1‑93 (MQ=255)

                                                     ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     >  2:2894271/1‑93 (MQ=255)

                                                     ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     <  1:80552/93‑1 (MQ=255)

                                                     ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     >  1:6374537/1‑93 (MQ=255)

                                                      cGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtt                                    <  1:50007/93‑1 (MQ=255)

                                                      cGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtt                                    >  1:6058985/1‑93 (MQ=255)

                                                       gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt                                   >  2:1218440/1‑93 (MQ=255)

                                                       gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt                                   >  2:3433915/1‑93 (MQ=255)

                                                        aTAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtttt                                  >  2:579624/1‑93 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 <  2:2358659/93‑1 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 >  1:440924/1‑93 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 >  2:1260599/1‑93 (MQ=255)

                                                           gAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa                               >  2:1427407/1‑93 (MQ=255)

                                                           gAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa                               >  2:3483395/1‑93 (MQ=255)

                                                             aaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAcc                             >  1:5585903/1‑93 (MQ=255)

                                                             aaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAcc                             >  2:5013132/1‑93 (MQ=255)

                                                              aaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCg                            >  1:6242999/1‑93 (MQ=255)

                                                               aCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCgg                           <  1:5853761/93‑1 (MQ=255)

                                                               aCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCgg                           >  2:3758408/1‑93 (MQ=255)

                                                               aCTCGCTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAcctg                           >  2:3205922/1‑91 (MQ=255)

                                                                cTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGc                          >  1:5114632/1‑93 (MQ=255)

                                                                 tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCTCCGGCa                         <  2:4619462/93‑1 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  1:3949657/93‑1 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  2:4086444/93‑1 (MQ=255)

                                                                    ttATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCg                      <  1:2875105/93‑1 (MQ=255)

                                                                     tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGTCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCgg                     >  2:3969348/1‑93 (MQ=255)

                                                                     tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCgc                     >  2:1007900/1‑92 (MQ=255)

                                                                      aTGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    >  2:5752034/1‑93 (MQ=255)

                                                                      aTGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    <  2:3927996/93‑1 (MQ=255)

                                                                        gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTa                  >  2:630422/1‑93 (MQ=255)

                                                                          cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaa                <  2:5143060/93‑1 (MQ=255)

                                                                           gTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAc               >  1:831190/1‑93 (MQ=255)

                                                                            tGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTACACg              >  2:353115/1‑93 (MQ=255)

                                                                            tGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACg              >  2:3118029/1‑93 (MQ=255)

                                                                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            >  1:5628060/1‑93 (MQ=255)

                                                                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            <  1:5341669/93‑1 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  2:707120/93‑1 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  1:1440240/93‑1 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  1:1649428/93‑1 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         <  1:5962273/93‑1 (MQ=255)

                                                                                   tGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTg       <  2:2415475/93‑1 (MQ=255)

                                                                                    gcgcCCCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  2:4845194/93‑1 (MQ=255)

                                                                                    gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  2:1942489/93‑1 (MQ=255)

                                                                                      gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGttt    <  1:1936777/93‑1 (MQ=255)

                                                                                      gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGttt    <  2:5999292/93‑1 (MQ=255)

                                                                                       ccACCTCCTTACGGGCTGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   <  1:1199996/93‑1 (MQ=255)

                                                                                       ccACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTACACGGATATTGCTTc   >  2:531812/1‑93 (MQ=255)

                                                                                       ccACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   <  1:2657140/93‑1 (MQ=255)

                                                                                       ccACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   >  1:2995899/1‑93 (MQ=255)

                                                                                       ccACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   >  1:5181134/1‑93 (MQ=255)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945258‑3945444

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑C                                                                                               <  SRR4302537.2720544/94‑1 (MQ=60)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑C                                                                                               >  SRR4302537.5839113/1‑94 (MQ=60)

  AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CCT                                                                                             <  SRR4302537.6520261/94‑1 (MQ=60)

   AGCGTCCCAGCCATTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑Cctt                                                                                            <  SRR4302537.4878915/94‑4 (MQ=60)

   AGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑Cctt                                                                                            <  SRR4302537.1962297/94‑4 (MQ=60)

      GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACG                                                                                          >  SRR4302537.4024113/1‑94 (MQ=60)

       TCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCTCCTCCTTACGG                                                                                         <  SRR4302537.1943131/94‑1 (MQ=60)

       TCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGG                                                                                         <  SRR4302537.1076440/94‑1 (MQ=60)

       TCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGG                                                                                         <  SRR4302537.5158368/94‑1 (MQ=60)

       TCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGG                                                                                         <  SRR4302537.2579702/94‑1 (MQ=60)

        CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGG                                                                                        >  SRR4302537.4262532/1‑94 (MQ=60)

           AGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGG                                                                                     >  SRR4302537.1277325/1‑94 (MQ=60)

           AGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGC                                                                                     >  SRR4302537.3006031/1‑94 (MQ=60)

            GCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGC                                                                                    >  SRR4302537.4261513/1‑94 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                  <  SRR4302537.574736/94‑1 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                  >  SRR4302537.3105148/1‑94 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                  >  SRR4302537.849760/1‑94 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 >  SRR4302537.3656782/1‑94 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 >  SRR4302537.562372/1‑94 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 >  SRR4302537.2399300/1‑94 (MQ=60)

                CTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGC                                                                                <  SRR4302537.3382078/94‑1 (MQ=60)

                 TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCC                                                                               >  SRR4302537.4347183/1‑94 (MQ=60)

                  GATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCA                                                                              <  SRR4302537.6051546/94‑1 (MQ=60)

                  GATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCA                                                                              >  SRR4302537.1598648/1‑94 (MQ=60)

                     TAAGCATACATTCCTACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGT                                                                           <  SRR4302537.5812106/94‑1 (MQ=60)

                     TAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGT                                                                           <  SRR4302537.3656286/94‑1 (MQ=60)

                      AAGCATACATTCCCACAACTAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTG                                                                          <  SRR4302537.2519221/94‑1 (MQ=60)

                      AAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTG                                                                          <  SRR4302537.368358/94‑1 (MQ=60)

                      AAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTG                                                                          <  SRR4302537.4373400/94‑1 (MQ=60)

                       AGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGC                                                                         >  SRR4302537.4935558/1‑94 (MQ=60)

                        GCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCT                                                                        >  SRR4302537.1600332/1‑94 (MQ=60)

                        GCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCT                                                                        >  SRR4302537.2778680/1‑94 (MQ=60)

                        GCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCT                                                                        <  SRR4302537.4732474/94‑1 (MQ=60)

                          ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                      >  SRR4302537.3743660/1‑94 (MQ=60)

                          ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                      <  SRR4302537.5517478/94‑1 (MQ=60)

                           TCCATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCCCCTCCTTACGGGCGGCCTGCCAGGTGCTCAT                                                                     <  SRR4302537.4731902/94‑1 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                   >  SRR4302537.2515095/1‑94 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                   >  SRR4302537.2554354/1‑94 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                   <  SRR4302537.4437129/94‑1 (MQ=60)

                                 CCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGT                                                               >  SRR4302537.5340098/1‑94 (MQ=60)

                                  CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                              >  SRR4302537.5288151/1‑94 (MQ=60)

                                   CACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTT                                                             <  SRR4302537.1571333/94‑1 (MQ=60)

                                    ACAACGAGGCGTTGACACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTC                                                            <  SRR4302537.5838259/94‑1 (MQ=60)

                                    ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGACAGGTGCTCATGAGCGTTTC                                                            >  SRR4302537.2950451/1‑94 (MQ=60)

                                    ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGACAGGTGCTCATGAGCGTTTA                                                            >  SRR4302537.2950453/1‑94 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302537.1731240/94‑1 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302537.1271114/94‑1 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          <  SRR4302537.5695823/94‑1 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          <  SRR4302537.3816495/94‑1 (MQ=60)

                                        CGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCA                                                        <  SRR4302537.1544415/94‑1 (MQ=60)

                                         GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                       <  SRR4302537.2974091/94‑1 (MQ=60)

                                         GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                       >  SRR4302537.4082089/1‑94 (MQ=60)

                                             CGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                   >  SRR4302537.5242764/1‑94 (MQ=60)

                                               CTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCA                                                 <  SRR4302537.1244041/94‑1 (MQ=60)

                                               CTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCA                                                 <  SRR4302537.2303984/94‑1 (MQ=60)

                                               CTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCA                                                 >  SRR4302537.527997/1‑94 (MQ=60)

                                               CTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCA                                                 >  SRR4302537.5798593/1‑94 (MQ=60)

                                                TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                >  SRR4302537.1755615/1‑94 (MQ=60)

                                                TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                <  SRR4302537.3121266/94‑1 (MQ=60)

                                                TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                >  SRR4302537.2145092/1‑94 (MQ=60)

                                                 GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               >  SRR4302537.1918145/1‑94 (MQ=60)

                                                 GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               <  SRR4302537.688331/94‑1 (MQ=60)

                                                 GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               <  SRR4302537.5987774/94‑1 (MQ=60)

                                                  GCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTA                                              >  SRR4302537.3335582/1‑94 (MQ=60)

                                                  GCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTA                                              >  SRR4302537.3124672/1‑94 (MQ=60)

                                                    CCCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCGCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                            <  SRR4302537.464692/94‑1 (MQ=60)

                                                    ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                            >  SRR4302537.6418012/1‑94 (MQ=60)

                                                     CCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTT                                           <  SRR4302537.81144/94‑1 (MQ=60)

                                                     CCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTT                                           >  SRR4302537.6100611/1‑94 (MQ=60)

                                                     CCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTT                                           <  SRR4302537.6220512/94‑1 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          <  SRR4302537.50377/94‑1 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302537.1502219/1‑94 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302537.5907443/1‑94 (MQ=60)

                                                        ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        >  SRR4302537.443984/1‑94 (MQ=60)

                                                        ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        >  SRR4302537.1587308/1‑94 (MQ=60)

                                                         TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTC                                       <  SRR4302537.6301966/94‑1 (MQ=60)

                                                         TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTC                                       <  SRR4302537.950407/94‑1 (MQ=60)

                                                         TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTC                                       <  SRR4302537.1745619/94‑1 (MQ=60)

                                                          AGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCGTGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      <  SRR4302537.512107/94‑1 (MQ=60)

                                                           GAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAC                                     <  SRR4302537.835656/94‑1 (MQ=60)

                                                            AAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACC                                    >  SRR4302537.5624094/1‑94 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCTCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   >  SRR4302537.5116078/1‑94 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   >  SRR4302537.6285910/1‑94 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   <  SRR4302537.1727057/94‑1 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   >  SRR4302537.3988829/1‑94 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   >  SRR4302537.4970096/1‑94 (MQ=60)

                                                              AACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                  <  SRR4302537.316595/94‑1 (MQ=60)

                                                               ACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                 >  SRR4302537.5149888/1‑94 (MQ=60)

                                                               ACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                 <  SRR4302537.5893918/94‑1 (MQ=60)

                                                                  CGTTATGTCGTGAGGTAAGCGACACATCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAGAGCAGAAGTTTTTCACCGGCAGC                              <  SRR4302537.6511096/94‑1 (MQ=60)

                                                                   GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             <  SRR4302537.3977280/94‑1 (MQ=60)

                                                                    TTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGG                            <  SRR4302537.2895283/94‑1 (MQ=60)

                                                                        GTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAA                        <  SRR4302537.2308843/94‑1 (MQ=60)

                                                                         TCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAA                       <  SRR4302537.3349774/94‑1 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      >  SRR4302537.2814167/1‑94 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      <  SRR4302537.5225805/94‑1 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      >  SRR4302537.2233897/1‑94 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      >  SRR4302537.836875/1‑94 (MQ=60)

                                                                            TGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    >  SRR4302537.6113952/1‑94 (MQ=60)

                                                                            TGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    <  SRR4302537.563951/94‑1 (MQ=60)

                                                                             GAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGA                   >  SRR4302537.5666563/1‑94 (MQ=60)

                                                                             GAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGA                   >  SRR4302537.2711507/1‑94 (MQ=60)

                                                                              AGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  <  SRR4302537.5378278/94‑1 (MQ=60)

                                                                               GGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 >  SRR4302537.1980230/1‑94 (MQ=60)

                                                                               GGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 >  SRR4302537.1337340/1‑94 (MQ=60)

                                                                                GTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                <  SRR4302537.1661001/94‑1 (MQ=60)

                                                                                GTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                <  SRR4302537.1450277/94‑1 (MQ=60)

                                                                                 TATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATT               <  SRR4302537.6003196/94‑1 (MQ=60)

                                                                                 TATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATT               <  SRR4302537.1108675/94‑1 (MQ=60)

                                                                                  ATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTG              <  SRR4302537.5228828/94‑1 (MQ=60)

                                                                                      GCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          >  SRR4302537.3016791/1‑94 (MQ=60)

                                                                                      GCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          >  SRR4302537.5216786/1‑94 (MQ=60)

                                                                                      GCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          <  SRR4302537.1950244/94‑1 (MQ=60)

                                                                                      GCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          <  SRR4302537.4448221/94‑1 (MQ=60)

                                                                                       CCACCTCCTTACGGGCTGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         <  SRR4302537.1208335/94‑1 (MQ=60)

                                                                                       CCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         <  SRR4302537.2675711/94‑1 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302537.1184100/1‑94 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302537.3980889/1‑94 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        <  SRR4302537.4386914/94‑1 (MQ=60)

                                                                                         ACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTTACCGGCAGCGGTTAAACGGAGATTGTTTCTGT       >  SRR4302537.1966263/1‑94 (MQ=60)

                                                                                         ACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTAACCGGCAGCGGTTAAACGGATATTGTTTCTGT       >  SRR4302537.5483411/1‑94 (MQ=60)

                                                                                          CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG      <  SRR4302537.4486762/94‑1 (MQ=60)

                                                                                          CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG      <  SRR4302537.1046695/94‑1 (MQ=60)

                                                                                          CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG      <  SRR4302537.4870279/94‑1 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     <  SRR4302537.1443527/91‑1 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     <  SRR4302537.221076/91‑1 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     >  SRR4302537.445526/4‑94 (MQ=60)

                                                                                            C‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCG   >  SRR4302537.3783299/1‑94 (MQ=60)

                                                                                              CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  SRR4302537.5171853/1‑94 (MQ=60)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945258‑3945444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`