Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,255,616 Δ1 bp intergenic (‑19/+312) yhaO ← / ← yhaP putative transport system permease protein/putative L‑serine dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,255,6150G.99.7% 1595.3 / ‑4.1 343intergenic (‑18/+313)yhaO/yhaPputative transport system permease protein/putative L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (133/209);  minor base C (1/0);  total (134/209)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 367 total aligned reads displayed.

TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCA  >  NC_000913/3255522‑3255703
                                                                                             |                                                                                        
tCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAg                                                                                          >  1:1011968/1‑93 (MQ=255)
ccacaaTCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAg                                                                                          <  2:2419697/89‑1 (MQ=255)
 cATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGAGCCGCCGGGGTCGAAgc                                                                                         >  1:7394430/1‑92 (MQ=255)
 cATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgc                                                                                         >  1:923740/1‑92 (MQ=255)
 cATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgc                                                                                         >  2:7696897/1‑92 (MQ=255)
      tCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTg                                                                                   >  2:1555666/1‑93 (MQ=255)
       cccccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGc                                                                                  <  1:6788590/91‑1 (MQ=255)
       cACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGc                                                                                  <  1:2997466/93‑1 (MQ=255)
        ccccAGCCGGTGTCCGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCCCGACCCGCCGGGGTCGAA‑GCGTCTGCa                                                                                 <  1:611560/92‑1 (MQ=255)
         cccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCGCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCaa                                                                                <  1:2413499/93‑1 (MQ=255)
         cccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCaa                                                                                <  1:512913/93‑1 (MQ=255)
         cccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCaa                                                                                <  1:4581459/93‑1 (MQ=255)
          ccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAt                                                                               <  1:7467743/93‑1 (MQ=255)
            cgCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa                                                                             <  2:302018/92‑1 (MQ=255)
            aGCCGTTGTCAGTACTCTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACACGCCGGGGTCGAA‑GCGTCTGCAATGa                                                                             <  2:5686802/93‑1 (MQ=255)
            aGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa                                                                             <  1:1110324/93‑1 (MQ=255)
            aGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa                                                                             <  2:1023019/93‑1 (MQ=255)
             gCCGGTGTCAGTACTGTCGAATTTGATCTCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAc                                                                            >  1:6030742/1‑93 (MQ=255)
             gCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAc                                                                            >  1:3263228/1‑93 (MQ=255)
                 gtgtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCt                                                                        <  2:8365829/93‑1 (MQ=255)
                  tgtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCtt                                                                       <  1:2289869/93‑1 (MQ=255)
                   gtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCttt                                                                      >  2:3134363/1‑93 (MQ=255)
                    tCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa                                                                     >  1:540322/1‑93 (MQ=255)
                     cAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCCCTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAt                                                                    <  1:2765840/93‑1 (MQ=255)
                     cAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAt                                                                    >  1:5014235/1‑93 (MQ=255)
                     cAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAt                                                                    <  2:4320200/93‑1 (MQ=255)
                      attACTGTCGAATTTGATCGCTTCTCGCCACTCCCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAtt                                                                   <  1:8144749/91‑1 (MQ=255)
                      aGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAtt                                                                   <  1:2065919/93‑1 (MQ=255)
                       gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTc                                                                  >  2:396327/1‑93 (MQ=255)
                       gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTc                                                                  >  1:2804460/1‑93 (MQ=255)
                        tACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCg                                                                 >  2:3419427/1‑93 (MQ=255)
                         aCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGa                                                                <  2:6739732/93‑1 (MQ=255)
                         aCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGa                                                                <  1:164959/93‑1 (MQ=255)
                          cTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAt                                                               <  2:2089379/93‑1 (MQ=255)
                          cTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAt                                                               <  2:6164772/93‑1 (MQ=255)
                          cTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAt                                                               >  1:4229938/1‑93 (MQ=255)
                            gTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGc                                                             >  2:8646537/1‑93 (MQ=255)
                             tCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCa                                                            >  2:2025599/1‑93 (MQ=255)
                              cGAATTTGATCCCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCaa                                                           <  1:6494691/93‑1 (MQ=255)
                               gAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAt                                                          <  2:3881560/93‑1 (MQ=255)
                                aaTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAtt                                                         >  1:8338633/1‑93 (MQ=255)
                                 aTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAttt                                                        <  1:6660546/93‑1 (MQ=255)
                                     gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAt                                                    <  1:7683308/93‑1 (MQ=255)
                                     gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAt                                                    >  2:8719212/1‑93 (MQ=255)
                                      aTCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATa                                                   <  1:724802/93‑1 (MQ=255)
                                      aTCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATa                                                   <  2:4926989/93‑1 (MQ=255)
                                      aTCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATa                                                   <  2:6382078/93‑1 (MQ=255)
                                       tCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa                                                  <  1:3636700/93‑1 (MQ=255)
                                       tCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa                                                  <  1:3535326/93‑1 (MQ=255)
                                        cGCTTCTCGCCACTCGCTCTCCCTCATTCCA‑GCACGACACGCCTGGGTCGAA‑GCGTCTGCAATGACGCCTTTATACGATTCAATTTCCATact                                                 >  2:2790420/1‑91 (MQ=255)
                                         gCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAtt                                                >  1:1310441/1‑93 (MQ=255)
                                          cTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAttt                                               >  1:4325389/1‑93 (MQ=255)
                                           ttCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtc                                              <  2:8100684/93‑1 (MQ=255)
                                            tctcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtct                                             <  1:415082/93‑1 (MQ=255)
                                            tctcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtct                                             >  2:169202/1‑93 (MQ=255)
                                             ctcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtctc                                            <  2:759811/93‑1 (MQ=255)
                                             ctcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtctc                                            <  2:7167133/93‑1 (MQ=255)
                                              tcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCg                                           >  1:8207028/1‑93 (MQ=255)
                                                gCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCt                                         >  2:7381039/1‑93 (MQ=255)
                                                 ccACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTc                                        <  1:3081921/93‑1 (MQ=255)
                                                  cACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCa                                       >  1:4823727/1‑93 (MQ=255)
                                                  cACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCa                                       <  1:5301795/93‑1 (MQ=255)
                                                   aCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCaa                                      >  1:5502935/1‑93 (MQ=255)
                                                   aCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCaa                                      >  2:4572929/1‑93 (MQ=255)
                                                   aCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCaa                                      >  1:6985634/1‑93 (MQ=255)
                                                    cTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAat                                     >  1:5627790/1‑93 (MQ=255)
                                                     tCGCTCTCACTCCTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAata                                    <  2:6849374/93‑1 (MQ=255)
                                                     tCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAata                                    >  1:6283314/1‑93 (MQ=255)
                                                     tCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAata                                    <  1:3366481/93‑1 (MQ=255)
                                                     tCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGGCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAata                                    >  1:7606424/1‑93 (MQ=255)
                                                      cGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAatat                                   >  2:1216050/1‑93 (MQ=255)
                                                       gCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAtt                                  <  2:7018881/93‑1 (MQ=255)
                                                           tcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGt                              >  2:8170796/1‑93 (MQ=255)
                                                            cactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTa                             <  2:4886846/93‑1 (MQ=255)
                                                             actcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAg                            <  2:5368290/93‑1 (MQ=255)
                                                              ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAgg                           <  1:7811221/93‑1 (MQ=255)
                                                              ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAgg                           <  2:6451106/93‑1 (MQ=255)
                                                               tcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAggg                          >  1:686142/1‑93 (MQ=255)
                                                                 aTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt                        >  1:5559889/1‑93 (MQ=255)
                                                                    tccagCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCtta                      <  2:1317009/89‑1 (MQ=255)
                                                                      cagCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCttatt                    <  1:1871420/91‑1 (MQ=255)
                                                                      cagCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCttatt                    >  1:3898215/3‑93 (MQ=255)
                                                                       agCACGGCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTATCGCTCAATATTTTGTAGGGCttatta                   <  2:3245614/92‑1 (MQ=255)
                                                                          aCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttt                >  2:2324579/1‑93 (MQ=255)
                                                                          aCGACCAGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttt                >  2:8554748/1‑93 (MQ=255)
                                                                           cgccccGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATTCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAtttt               <  2:1827896/90‑1 (MQ=255)
                                                                           cGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAtttt               <  2:6006553/93‑1 (MQ=255)
                                                                           cGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAtttt               >  1:44242/1‑93 (MQ=255)
                                                                           cGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAtttt               >  2:779664/1‑93 (MQ=255)
                                                                              cccGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttttttt            <  1:2187048/93‑1 (MQ=255)
                                                                              cccGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAttttttt            <  2:4172756/93‑1 (MQ=255)
                                                                               ccgccgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTc           <  1:5782360/93‑1 (MQ=255)
                                                                               ccgccgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTc           >  1:8409841/1‑93 (MQ=255)
                                                                                cgccgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTcc          <  1:5453350/93‑1 (MQ=255)
                                                                                   cgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAg       <  1:2616459/93‑1 (MQ=255)
                                                                                    ggggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGc      >  2:5216519/1‑93 (MQ=255)
                                                                                    ggggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGc      >  2:8225371/1‑93 (MQ=255)
                                                                                    ggggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGc      >  1:8648991/1‑93 (MQ=255)
                                                                                      ggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCg    >  1:8163382/1‑93 (MQ=255)
                                                                                        tCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCa  >  2:3692465/1‑93 (MQ=255)
                                                                                             |                                                                                        
TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCA  >  NC_000913/3255522‑3255703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 211 total aligned reads displayed.
TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGG  >  NC_000913/3255522‑3255709
                                                                                              |                                                                                             
TCATAATCCCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAGC                                                                                               <  SRR4302541.3901329/94‑1 (MQ=60)
TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGAGCCGCCGGGGTCGAAGC                                                                                               >  SRR4302541.7473500/1‑94 (MQ=60)
TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAGC                                                                                               >  SRR4302541.932407/1‑94 (MQ=60)
TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAGC                                                                                               <  SRR4302541.218344/94‑1 (MQ=60)
  ATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGT                                                                                            >  SRR4302541.110705/1‑94 (MQ=60)
    AATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCT                                                                                          <  SRR4302541.2923237/94‑1 (MQ=60)
    AATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCT                                                                                          >  SRR4302541.5023879/1‑94 (MQ=60)
     ATCCCCCCGCCGGTTTCCGTACTGGCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTG                                                                                         <  SRR4302541.4510752/94‑1 (MQ=60)
     ATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTG                                                                                         <  SRR4302541.4510753/94‑1 (MQ=60)
      TCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGC                                                                                        >  SRR4302541.6314285/1‑94 (MQ=60)
       CCCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCA                                                                                       <  SRR4302541.6861182/94‑1 (MQ=60)
       CCCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCA                                                                                       <  SRR4302541.7237993/94‑1 (MQ=60)
       CACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGCCCCGCCGGGGCCGAA‑GCGTCTGCA                                                                                       >  SRR4302541.6140361/1‑94 (MQ=60)
       CACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCA                                                                                       <  SRR4302541.3027720/94‑1 (MQ=60)
        CCCCAGCCGGTGTCCGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCCCGACCCGCCGGGGTCGAA‑GCGTCTGCAA                                                                                      <  SRR4302541.617307/94‑1 (MQ=60)
        ACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAA                                                                                      >  SRR4302541.6458921/1‑94 (MQ=60)
         CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCGCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAT                                                                                     <  SRR4302541.2437250/94‑1 (MQ=60)
         CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAT                                                                                     <  SRR4302541.4627884/94‑1 (MQ=60)
         CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAT                                                                                     <  SRR4302541.517708/94‑1 (MQ=60)
         CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAT                                                                                     <  SRR4302541.364141/94‑1 (MQ=60)
         CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAT                                                                                     >  SRR4302541.3524230/1‑94 (MQ=60)
          CCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATG                                                                                    <  SRR4302541.2197302/94‑1 (MQ=60)
          CCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATG                                                                                    <  SRR4302541.4309305/94‑1 (MQ=60)
          CCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATG                                                                                    <  SRR4302541.7547579/94‑1 (MQ=60)
          CCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATG                                                                                    <  SRR4302541.7864709/94‑1 (MQ=60)
          CCAGCCGCTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCTCTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATG                                                                                    <  SRR4302541.7864708/94‑1 (MQ=60)
          CAAGCCGGTGTCAGTCATGTCGAATTTGAGCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATG                                                                                    <  SRR4302541.5194726/94‑1 (MQ=60)
           CAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGA                                                                                   >  SRR4302541.942910/1‑94 (MQ=60)
           CAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGA                                                                                   >  SRR4302541.1986258/1‑94 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCTCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  >  SRR4302541.6092512/1‑94 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  <  SRR4302541.2402631/94‑1 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  <  SRR4302541.5675170/94‑1 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  >  SRR4302541.3296154/1‑94 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  <  SRR4302541.7277456/94‑1 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  <  SRR4302541.8452885/94‑1 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  <  SRR4302541.8705601/94‑1 (MQ=60)
            AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                                                  <  SRR4302541.1120754/94‑1 (MQ=60)
             GCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACG                                                                                 >  SRR4302541.4161857/1‑94 (MQ=60)
              CCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGC                                                                                >  SRR4302541.5855285/1‑94 (MQ=60)
               CGGTTTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCC                                                                               <  SRR4302541.5504590/94‑1 (MQ=60)
                 GTGTCAGTCCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTT                                                                             <  SRR4302541.52933/94‑1 (MQ=60)
                 GTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTT                                                                             <  SRR4302541.701663/94‑1 (MQ=60)
                 GTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGAGACGAA‑GCGTCTGCAATGACGCCTT                                                                             >  SRR4302541.3865923/1‑94 (MQ=60)
                  gagaacTACTGTCGAATTTGATCGCTACACGACCCTCGCTCTCTCTCATTCCA‑GCCGGACCCGCCGGGGTCGAA‑GCGTCTGCACTGACGCCTTT                                                                            <  SRR4302541.558808/88‑1 (MQ=24)
                  TGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTT                                                                            <  SRR4302541.2741820/94‑1 (MQ=60)
                  TGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTT                                                                            <  SRR4302541.558809/94‑1 (MQ=60)
                  TGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTT                                                                            <  SRR4302541.6801606/94‑1 (MQ=60)
                  TGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTT                                                                            <  SRR4302541.2312042/94‑1 (MQ=60)
                   GTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTA                                                                           >  SRR4302541.545401/1‑94 (MQ=60)
                    TCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAT                                                                          >  SRR4302541.5065781/1‑94 (MQ=60)
                    TCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAT                                                                          <  SRR4302541.1908822/94‑1 (MQ=60)
                    TCAGTACTGTCGAATGTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAT                                                                          <  SRR4302541.1814783/94‑1 (MQ=60)
                     CAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCCCTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATT                                                                         <  SRR4302541.2793678/94‑1 (MQ=60)
                     CAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATT                                                                         <  SRR4302541.6449232/94‑1 (MQ=60)
                      ATTACTGTCGAATTTGATCGCTTCTCGCCACTCCCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                                        <  SRR4302541.8233833/94‑1 (MQ=60)
                      AGTTCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                                        <  SRR4302541.149154/94‑1 (MQ=60)
                      AGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                                        <  SRR4302541.2085874/94‑1 (MQ=60)
                      AGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                                        <  SRR4302541.1182321/94‑1 (MQ=60)
                      AGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                                        >  SRR4302541.7811027/1‑94 (MQ=60)
                      AGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                                        >  SRR4302541.2832822/1‑94 (MQ=60)
                       GTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCG                                                                       <  SRR4302541.8272660/94‑1 (MQ=60)
                         ACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAT                                                                     >  SRR4302541.4272079/1‑94 (MQ=60)
                         ACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAT                                                                     <  SRR4302541.166193/94‑1 (MQ=60)
                          CTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATG                                                                    >  SRR4302541.6810054/1‑94 (MQ=60)
                          CTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATG                                                                    <  SRR4302541.1265542/94‑1 (MQ=60)
                           TGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGC                                                                   >  SRR4302541.4821680/1‑94 (MQ=60)
                            GTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCA                                                                  >  SRR4302541.7351625/1‑94 (MQ=60)
                            GTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGCCTGCAATGACGCCTTTATTCGATGCA                                                                  >  SRR4302541.8278793/1‑94 (MQ=60)
                              CGAATTTGATCCCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAT                                                                <  SRR4302541.6560901/94‑1 (MQ=60)
                               GAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATT                                                               >  SRR4302541.3962206/1‑94 (MQ=60)
                               GAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATT                                                               >  SRR4302541.7525891/1‑94 (MQ=60)
                               GAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATT                                                               >  SRR4302541.574479/1‑94 (MQ=60)
                               GAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATT                                                               >  SRR4302541.8429175/1‑94 (MQ=60)
                                AATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTT                                                              <  SRR4302541.8247743/94‑1 (MQ=60)
                                AATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTT                                                              >  SRR4302541.5145927/1‑94 (MQ=60)
                                AATTGGATCGCTTCTCGCCACCAGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTT                                                              <  SRR4302541.8096299/94‑1 (MQ=60)
                                 ATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTC                                                             >  SRR4302541.7658738/1‑94 (MQ=60)
                                 ATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTC                                                             >  SRR4302541.7658737/1‑94 (MQ=60)
                                 ATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTC                                                             <  SRR4302541.6728885/94‑1 (MQ=60)
                                  TTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCC                                                            <  SRR4302541.1564449/94‑1 (MQ=60)
                                  TTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCC                                                            <  SRR4302541.5757761/94‑1 (MQ=60)
                                   TTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCA                                                           <  SRR4302541.4669286/94‑1 (MQ=60)
                                   TTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCA                                                           <  SRR4302541.5090751/94‑1 (MQ=60)
                                   TTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCA                                                           <  SRR4302541.6606438/94‑1 (MQ=60)
                                    TGATCGCTTTTCGCCACTCGCTCTCACTCATTCCA‑GCCCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAT                                                          <  SRR4302541.7628880/94‑1 (MQ=60)
                                     TATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATA                                                         <  SRR4302541.2986902/94‑1 (MQ=60)
                                     GATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATA                                                         >  SRR4302541.6150432/1‑94 (MQ=60)
                                     GATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATA                                                         <  SRR4302541.7764992/94‑1 (MQ=60)
                                     GATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTAGATGCAATTTCCATA                                                         >  SRR4302541.3277591/1‑94 (MQ=60)
                                     GATCGCTTCTCGCCAATCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATA                                                         >  SRR4302541.3277590/1‑94 (MQ=60)
                                      ATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAA                                                        >  SRR4302541.6192433/1‑94 (MQ=60)
                                      ATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAA                                                        <  SRR4302541.731669/94‑1 (MQ=60)
                                       TCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAT                                                       <  SRR4302541.3570966/94‑1 (MQ=60)
                                       TCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAT                                                       <  SRR4302541.3673574/94‑1 (MQ=60)
                                        CGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATT                                                      >  SRR4302541.1323343/1‑94 (MQ=60)
                                         GCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTT                                                     >  SRR4302541.6698287/1‑94 (MQ=60)
                                         GCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTT                                                     >  SRR4302541.4368576/1‑94 (MQ=60)
                                           TTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCT                                                   <  SRR4302541.4213002/94‑1 (MQ=60)
                                           TTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCT                                                   <  SRR4302541.28164/94‑1 (MQ=60)
                                            TCTCGCCCCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTC                                                  <  SRR4302541.6751006/94‑1 (MQ=60)
                                            TCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTC                                                  <  SRR4302541.418930/94‑1 (MQ=60)
                                            TCTCGCCACGCTCTCCCCCTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTACTCGATGCAATTTCCATAATTTCTC                                                  <  SRR4302541.7846709/94‑1 (MQ=60)
                                             CTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCG                                                 <  SRR4302541.547255/94‑1 (MQ=60)
                                             CTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCG                                                 <  SRR4302541.2961367/94‑1 (MQ=60)
                                             CTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCG                                                 >  SRR4302541.8296569/1‑94 (MQ=60)
                                             CTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCG                                                 <  SRR4302541.8817491/94‑1 (MQ=60)
                                             CTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCG                                                 >  SRR4302541.3578434/1‑94 (MQ=60)
                                               CGCCCCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCT                                               <  SRR4302541.4627226/94‑1 (MQ=60)
                                               CGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCT                                               <  SRR4302541.2230961/94‑1 (MQ=60)
                                               CGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCT                                               >  SRR4302541.2563496/1‑94 (MQ=60)
                                               CGCCACTCCCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCT                                               <  SRR4302541.8254456/94‑1 (MQ=60)
                                                GCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTC                                              <  SRR4302541.4463637/94‑1 (MQ=60)
                                                GCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTC                                              >  SRR4302541.2744621/1‑94 (MQ=60)
                                                 CCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCA                                             >  SRR4302541.4872895/1‑94 (MQ=60)
                                                 CCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCA                                             <  SRR4302541.3113198/94‑1 (MQ=60)
                                                  CACTCGCTCTCACTCATTCCAGG‑ACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAA                                            >  SRR4302541.1539449/1‑94 (MQ=60)
                                                  CACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAA                                            >  SRR4302541.5559303/1‑94 (MQ=60)
                                                  CACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAA                                            >  SRR4302541.419755/1‑94 (MQ=60)
                                                  CACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAA                                            <  SRR4302541.6936759/94‑1 (MQ=60)
                                                  CACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAA                                            >  SRR4302541.7060032/1‑94 (MQ=60)
                                                  CACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAA                                            <  SRR4302541.5356366/94‑1 (MQ=60)
                                                   ACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAAT                                           >  SRR4302541.5685361/1‑94 (MQ=60)
                                                   ACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAAT                                           >  SRR4302541.8417240/1‑94 (MQ=60)
                                                    CTCGCTCTCACTCATTCCA‑GCACGACCCGGCGGGGTCGAA‑GCGTCTGCAATGAGGCCTTTATTCTATGCAATTTCCATAATTTTTCGCTCAATT                                          >  SRR4302541.7281554/1‑94 (MQ=60)
                                                    CTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATA                                          >  SRR4302541.6347499/1‑94 (MQ=60)
                                                    CTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATA                                          >  SRR4302541.2538924/1‑94 (MQ=60)
                                                    CTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGGCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATA                                          >  SRR4302541.7687698/1‑94 (MQ=60)
                                                     TCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAT                                         >  SRR4302541.2368068/1‑94 (MQ=60)
                                                     TCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAT                                         <  SRR4302541.3400064/94‑1 (MQ=60)
                                                      CGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATT                                        <  SRR4302541.2826438/94‑1 (MQ=60)
                                                      CGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATT                                        >  SRR4302541.5498475/1‑94 (MQ=60)
                                                       GCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTT                                       >  SRR4302541.3371301/1‑94 (MQ=60)
                                                        CTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGGCTGCAATGACGCCTTTATTCGATGCAAGTTCCATAATTTCTCGCTCAATATTTT                                      >  SRR4302541.1278203/1‑94 (MQ=60)
                                                         TCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTG                                     <  SRR4302541.325642/94‑1 (MQ=60)
                                                         TCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTG                                     <  SRR4302541.5544322/94‑1 (MQ=60)
                                                         TCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTCCCATAATTTCTCGCTCAATATTTTG                                     >  SRR4302541.5802883/1‑94 (MQ=60)
                                                          CTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGT                                    <  SRR4302541.5359046/94‑1 (MQ=60)
                                                          CTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGT                                    >  SRR4302541.5406919/1‑94 (MQ=60)
                                                           TCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTA                                   <  SRR4302541.4839888/94‑1 (MQ=60)
                                                            CACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAG                                  >  SRR4302541.6132789/1‑94 (MQ=60)
                                                            CACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAG                                  >  SRR4302541.929024/1‑94 (MQ=60)
                                                            CACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAG                                  <  SRR4302541.3096973/94‑1 (MQ=60)
                                                            CACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAG                                  <  SRR4302541.1808715/94‑1 (MQ=60)
                                                             CCTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGG                                 <  SRR4302541.8611797/94‑1 (MQ=60)
                                                              CTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGG                                >  SRR4302541.692603/1‑94 (MQ=60)
                                                              CTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGG                                >  SRR4302541.8250185/1‑94 (MQ=60)
                                                              CTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGG                                <  SRR4302541.7895648/94‑1 (MQ=60)
                                                              CTCATTCCA‑GCACGACCCGCCGGGGTCG‑ACGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCCTAATTTCTCGCTCAATAATTTGTAGTG                                >  SRR4302541.692602/1‑94 (MQ=60)
                                                                 cataccaGCACGGCCCGCCGAGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCT                              <  SRR4302541.8349273/87‑1 (MQ=60)
                                                                 ATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTT                             <  SRR4302541.3084377/94‑1 (MQ=60)
                                                                  TTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTA                            <  SRR4302541.3384411/94‑1 (MQ=60)
                                                                  TTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTA                            <  SRR4302541.2576649/94‑1 (MQ=60)
                                                                   TCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTAT                           <  SRR4302541.5889984/94‑1 (MQ=60)
                                                                    CCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATT                          >  SRR4302541.3351268/1‑94 (MQ=60)
                                                                    CCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATT                          >  SRR4302541.3937236/1‑94 (MQ=60)
                                                                      caGCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTA                         <  SRR4302541.1889438/92‑1 (MQ=60)
                                                                      caGCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTA                         <  SRR4302541.3616208/92‑1 (MQ=60)
                                                                       agccCGACCCGGCGGGGGCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAT                        <  SRR4302541.6897627/90‑1 (MQ=60)
                                                                         CACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTT                      >  SRR4302541.5523963/1‑94 (MQ=60)
                                                                          ACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTT                     >  SRR4302541.44576/1‑94 (MQ=60)
                                                                          ACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTT                     >  SRR4302541.4263712/1‑94 (MQ=60)
                                                                           CGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTT                    >  SRR4302541.5018476/1‑94 (MQ=60)
                                                                           CGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTT                    <  SRR4302541.44424/94‑1 (MQ=60)
                                                                           CGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTACTCGCTCAATATTTTGTAGGGCTTATTATTTTT                    >  SRR4302541.8561672/1‑94 (MQ=60)
                                                                             ACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTT                  <  SRR4302541.7088065/94‑1 (MQ=60)
                                                                             ACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTT                  >  SRR4302541.7538047/1‑94 (MQ=60)
                                                                              CCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTC                 <  SRR4302541.2208193/94‑1 (MQ=60)
                                                                              CCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTC                 >  SRR4302541.8500789/1‑94 (MQ=60)
                                                                               CCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCC                <  SRR4302541.7725330/94‑1 (MQ=60)
                                                                               CCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCC                <  SRR4302541.5841143/94‑1 (MQ=60)
                                                                               CCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCC                <  SRR4302541.2273215/94‑1 (MQ=60)
                                                                                CGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCG               <  SRR4302541.5509300/94‑1 (MQ=60)
                                                                                CGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCG               <  SRR4302541.5509299/94‑1 (MQ=60)
                                                                                  CCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAG             <  SRR4302541.91940/94‑1 (MQ=60)
                                                                                  CCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAG             <  SRR4302541.8127104/94‑1 (MQ=60)
                                                                                   CGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGC            >  SRR4302541.5659660/1‑94 (MQ=60)
                                                                                   CGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGC            <  SRR4302541.7543433/94‑1 (MQ=60)
                                                                                   CGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGC            <  SRR4302541.8470331/94‑1 (MQ=60)
                                                                                   CGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGC            >  SRR4302541.194353/1‑94 (MQ=60)
                                                                                   CGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGC            >  SRR4302541.7340281/1‑94 (MQ=60)
                                                                                   CGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGC            <  SRR4302541.2642593/94‑1 (MQ=60)
                                                                                   CGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGC            >  SRR4302541.8741604/1‑94 (MQ=60)
                                                                                    GGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCC           >  SRR4302541.5573354/1‑94 (MQ=60)
                                                                                    GGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCC           >  SRR4302541.8391018/1‑94 (MQ=60)
                                                                                    GGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCC           <  SRR4302541.43267/94‑1 (MQ=60)
                                                                                     GGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCG          >  SRR4302541.8252556/1‑94 (MQ=60)
                                                                                     GGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCG          >  SRR4302541.3784420/1‑94 (MQ=60)
                                                                                       GTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCA        <  SRR4302541.2322029/94‑1 (MQ=60)
                                                                                        TCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCAT       <  SRR4302541.1104442/94‑1 (MQ=60)
                                                                                        TCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCAT       >  SRR4302541.8482492/1‑94 (MQ=60)
                                                                                         CGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATC      >  SRR4302541.311193/1‑94 (MQ=60)
                                                                                         CGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATC      >  SRR4302541.8526513/1‑94 (MQ=60)
                                                                                          GAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCA     <  SRR4302541.8140545/94‑1 (MQ=60)
                                                                                          GAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCA     >  SRR4302541.1829489/1‑94 (MQ=60)
                                                                                          GAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCA     <  SRR4302541.6821067/94‑1 (MQ=60)
                                                                                            AAGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAA    >  SRR4302541.5743191/1‑94 (MQ=60)
                                                                                             AGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAG   >  SRR4302541.3715256/1‑94 (MQ=60)
                                                                                             AGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAG   >  SRR4302541.6101277/1‑94 (MQ=60)
                                                                                              GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGG  <  SRR4302541.6747684/94‑1 (MQ=60)
                                                                                              GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGG  <  SRR4302541.6816757/94‑1 (MQ=60)
                                                                                              |                                                                                             
TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGG  >  NC_000913/3255522‑3255709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑