Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,279,197 A→C R145S (AGA→AGC)  agaA → putative N‑acetylgalactosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,279,1970AC99.7% 1131.6 / ‑5.2 331R145S (AGA→AGC) agaAputative N‑acetylgalactosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (166/164);  total (166/165)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

Only 100 of 350 total aligned reads displayed.

GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286
                                                                                          |                                                                                         
ggAAGCCTTCAATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         <  2:3194898/93‑1 (MQ=255)
ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         >  2:8545598/1‑93 (MQ=255)
ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         >  2:7616927/1‑93 (MQ=255)
  aaGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCg                                                                                       <  2:800160/93‑1 (MQ=255)
   aGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt                                                                                      <  2:5281343/93‑1 (MQ=255)
   aGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt                                                                                      >  1:6708104/1‑93 (MQ=255)
    gCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt                                                                                     <  1:7664823/93‑1 (MQ=255)
      catccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc                                                                                   <  2:270028/93‑1 (MQ=255)
      catccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc                                                                                   >  1:5678655/1‑93 (MQ=255)
       atccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcg                                                                                  >  1:1649171/1‑93 (MQ=255)
        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 <  1:1351401/93‑1 (MQ=255)
        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 <  2:3905110/93‑1 (MQ=255)
        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 <  1:4605235/93‑1 (MQ=255)
         ccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt                                                                                >  2:130167/1‑93 (MQ=255)
         ccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt                                                                                >  2:6855434/1‑93 (MQ=255)
           atatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg                                                                              <  1:4054636/93‑1 (MQ=255)
           atatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg                                                                              <  2:2326713/93‑1 (MQ=255)
           atatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg                                                                              >  2:179317/1‑93 (MQ=255)
           atatGGCGTCGCTGAATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg                                                                              <  2:4008903/93‑1 (MQ=255)
            tatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAGCGCGCCAGCGTCGTTGCGCTGGa                                                                             <  1:2806389/93‑1 (MQ=255)
             atGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt                                                                            <  1:2670486/93‑1 (MQ=255)
              tGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa                                                                           >  1:1532640/1‑93 (MQ=255)
              tGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa                                                                           <  1:4630861/93‑1 (MQ=255)
               ggCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg                                                                          <  2:5468868/93‑1 (MQ=255)
                  gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg                                                                       >  1:8693696/1‑93 (MQ=255)
                   tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg                                                                      >  1:3876180/1‑93 (MQ=255)
                       tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc                                                                  >  1:8211158/1‑93 (MQ=255)
                         cATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt                                                                >  1:6556743/1‑93 (MQ=255)
                          ctCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg                                                               <  2:7000256/92‑1 (MQ=255)
                           tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt                                                              >  1:3221128/1‑93 (MQ=255)
                           tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt                                                              >  2:1059033/1‑93 (MQ=255)
                           tCCGGCGCGAATGCGGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt                                                              <  1:4769144/93‑1 (MQ=255)
                               gcgcGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaa                                                          <  2:3741128/93‑1 (MQ=255)
                                cccgAATGCTGGGTGTTGATGGTGTTCTTGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGccac                                                         <  2:2904408/91‑1 (MQ=255)
                                 gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca                                                        <  1:840130/93‑1 (MQ=255)
                                  cgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaacaa                                                       <  2:3571998/93‑1 (MQ=255)
                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      <  2:7311311/93‑1 (MQ=255)
                                    aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt                                                     <  2:4583425/93‑1 (MQ=255)
                                    aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt                                                     >  1:4121545/1‑93 (MQ=255)
                                      ttcTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   >  2:6481939/3‑93 (MQ=255)
                                      tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   >  2:1353544/1‑93 (MQ=255)
                                      tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   >  1:2965665/1‑93 (MQ=255)
                                        cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg                                                 >  2:3519322/1‑93 (MQ=255)
                                         tGGGTGTTGATGTTGTTCTGGGATCGCTTAAACCGGGGAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa                                                <  2:6095906/93‑1 (MQ=255)
                                         tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTggt                                                >  1:2170841/1‑92 (MQ=255)
                                         tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa                                                <  1:5537746/93‑1 (MQ=255)
                                         tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa                                                <  2:5179471/93‑1 (MQ=255)
                                           ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt                                              >  1:3406635/1‑93 (MQ=255)
                                           ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt                                              >  2:6249632/1‑93 (MQ=255)
                                           ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt                                              >  2:1914843/1‑93 (MQ=255)
                                             tgtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa                                            <  2:4941206/93‑1 (MQ=255)
                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           <  2:2367784/93‑1 (MQ=255)
                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           <  2:2120670/93‑1 (MQ=255)
                                                tGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg                                         <  2:3097616/93‑1 (MQ=255)
                                                tGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg                                         >  1:6521400/1‑93 (MQ=255)
                                                 gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCCACAAATCTGGATTCAGGGt                                        >  1:8574076/1‑93 (MQ=255)
                                                  aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc                                       >  1:712775/1‑93 (MQ=255)
                                                  aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc                                       <  1:4484894/93‑1 (MQ=255)
                                                    ggTGTTCTGGGATCGCTTAAACCTGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCaa                                     <  2:4746342/93‑1 (MQ=255)
                                                    ggTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCaa                                     <  2:5916479/93‑1 (MQ=255)
                                                         tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGc                                >  2:1503928/1‑93 (MQ=255)
                                                          cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt                               <  2:606502/93‑1 (MQ=255)
                                                             ggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg                            >  1:2028161/1‑93 (MQ=255)
                                                               aTCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt                          >  1:1117170/1‑93 (MQ=255)
                                                               aTCGCTTAAACCGGGCAAACGCACCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCACCACATCTGGATTCAGGGTCAATTAGCTTCGtt                          <  1:7797535/93‑1 (MQ=255)
                                                                tCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt                         <  1:4391343/93‑1 (MQ=255)
                                                                 cGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtttt                        >  2:8237141/1‑93 (MQ=255)
                                                                 cGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtttt                        <  1:8742136/93‑1 (MQ=255)
                                                                   cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCGATTAGCTTCGTTTTGa                      >  2:4852142/1‑93 (MQ=255)
                                                                    ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt                     <  1:5828062/93‑1 (MQ=255)
                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    >  2:7115494/1‑93 (MQ=255)
                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  2:8659273/93‑1 (MQ=255)
                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  2:5990336/93‑1 (MQ=255)
                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  1:987503/93‑1 (MQ=255)
                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  1:5745137/93‑1 (MQ=255)
                                                                      aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAg                   <  2:2548025/93‑1 (MQ=255)
                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  <  1:5958780/93‑1 (MQ=255)
                                                                        aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGtt                 <  1:189871/93‑1 (MQ=255)
                                                                        aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGtt                 <  1:5148549/93‑1 (MQ=255)
                                                                         ccGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGttt                <  2:3331051/93‑1 (MQ=255)
                                                                           gggCAANCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              >  1:6174859/1‑93 (MQ=255)
                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              >  1:4143274/1‑93 (MQ=255)
                                                                            ggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCAGGATGCAGGGTCAATTAGCTTCGTTTCGATAGTTTGCt             >  2:7841162/1‑93 (MQ=255)
                                                                             gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTc            >  2:445096/1‑93 (MQ=255)
                                                                               aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCt          <  1:1481002/93‑1 (MQ=255)
                                                                               aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCt          >  1:2867800/1‑93 (MQ=255)
                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         <  1:5650220/93‑1 (MQ=255)
                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         >  1:4897548/1‑93 (MQ=255)
                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         <  2:8322939/93‑1 (MQ=255)
                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         >  1:8257958/1‑93 (MQ=255)
                                                                                   gcgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAt      <  1:5291779/93‑1 (MQ=255)
                                                                                    cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAtt     >  2:7396992/1‑93 (MQ=255)
                                                                                    cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAtt     <  1:7981426/93‑1 (MQ=255)
                                                                                     gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTg    <  2:6783612/93‑1 (MQ=255)
                                                                                     gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTg    <  1:6374639/93‑1 (MQ=255)
                                                                                     gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTg    <  1:7420143/93‑1 (MQ=255)
                                                                                     gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTg    >  2:7723280/1‑93 (MQ=255)
                                                                                     gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTg    <  2:1632398/93‑1 (MQ=255)
                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   >  1:7552233/1‑93 (MQ=255)
                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   >  1:7952169/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279105‑3279290
                                                                                            |                                                                                             
GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCG                                                                                              >  SRR4302541.4318664/1‑94 (MQ=60)
 CGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGT                                                                                             >  SRR4302541.8346553/1‑94 (MQ=60)
  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302541.6578907/1‑94 (MQ=60)
  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302541.6148578/1‑94 (MQ=60)
  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302541.5813590/1‑94 (MQ=60)
  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302541.4443099/1‑94 (MQ=60)
    AAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGT                                                                                          >  SRR4302541.6779006/1‑94 (MQ=60)
    AAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGT                                                                                          <  SRR4302541.8388610/94‑1 (MQ=60)
    AAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGT                                                                                          >  SRR4302541.3596299/1‑94 (MQ=60)
     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         <  SRR4302541.2265362/94‑1 (MQ=60)
     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         >  SRR4302541.7985443/1‑94 (MQ=60)
     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         >  SRR4302541.3993228/1‑94 (MQ=60)
      GCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        <  SRR4302541.7746373/94‑1 (MQ=60)
       CCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGC                                                                                       >  SRR4302541.5736595/1‑94 (MQ=60)
        CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      >  SRR4302541.1665460/1‑94 (MQ=60)
        CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      >  SRR4302541.3343246/1‑94 (MQ=60)
        CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      >  SRR4302541.4531147/1‑94 (MQ=60)
        CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      <  SRR4302541.8183721/94‑1 (MQ=60)
         ATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGC                                                                                     >  SRR4302541.8496729/1‑94 (MQ=60)
          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    >  SRR4302541.7528243/1‑94 (MQ=60)
          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302541.4651983/94‑1 (MQ=60)
          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302541.1364757/94‑1 (MQ=60)
             ATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGA                                                                                 <  SRR4302541.4094644/94‑1 (MQ=60)
             ATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGA                                                                                 <  SRR4302541.8685286/94‑1 (MQ=60)
              TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAGCGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302541.2834774/94‑1 (MQ=60)
              TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302541.2834875/94‑1 (MQ=60)
              TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGCGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                >  SRR4302541.6925542/1‑94 (MQ=60)
               ATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATA                                                                               <  SRR4302541.2697025/94‑1 (MQ=60)
               ATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATA                                                                               >  SRR4302541.1548004/1‑94 (MQ=60)
               ATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATA                                                                               <  SRR4302541.8044018/94‑1 (MQ=60)
                TGGCGTCGCTTCATCCTGCGCGAATGCTGGTTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302541.4366002/94‑1 (MQ=60)
                TGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302541.4677981/94‑1 (MQ=60)
                 GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             >  SRR4302541.4544159/1‑94 (MQ=60)
                  GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302541.7710401/94‑1 (MQ=60)
                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           >  SRR4302541.4385443/1‑94 (MQ=60)
                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           >  SRR4302541.8786755/1‑94 (MQ=60)
                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302541.947332/94‑1 (MQ=60)
                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302541.3915126/1‑94 (MQ=60)
                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          <  SRR4302541.6059029/94‑1 (MQ=60)
                      CGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGTATAGCGGGCT                                                                        >  SRR4302541.4669136/1‑94 (MQ=60)
                      CGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCT                                                                        >  SRR4302541.4918383/1‑94 (MQ=60)
                      CGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCT                                                                        >  SRR4302541.4001448/1‑94 (MQ=60)
                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       >  SRR4302541.4760348/1‑94 (MQ=60)
                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       >  SRR4302541.8295204/1‑94 (MQ=60)
                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       <  SRR4302541.8912167/94‑1 (MQ=60)
                        CTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAC                                                                      >  SRR4302541.8300738/1‑94 (MQ=60)
                        CTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAC                                                                      >  SRR4302541.7702228/1‑94 (MQ=60)
                          GCATCCGGCGCGAGTGCTGGGTGTTGATGGTTTTCTGGCATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    <  SRR4302541.7387697/94‑1 (MQ=60)
                          GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    >  SRR4302541.6623772/1‑94 (MQ=60)
                          GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    <  SRR4302541.5287096/94‑1 (MQ=60)
                            ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  >  SRR4302541.3253658/1‑94 (MQ=60)
                            ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  >  SRR4302541.1749178/1‑94 (MQ=60)
                             TCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTG                                                                 <  SRR4302541.4648883/94‑1 (MQ=60)
                             TCCGGCGCGAATGCGGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTG                                                                 <  SRR4302541.4817843/94‑1 (MQ=60)
                               CGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCA                                                               >  SRR4302541.1361923/1‑94 (MQ=60)
                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302541.290443/94‑1 (MQ=60)
                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302541.1451195/94‑1 (MQ=60)
                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302541.5555077/94‑1 (MQ=60)
                                   GCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAA                                                           >  SRR4302541.5481245/1‑94 (MQ=60)
                                   GCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAA                                                           <  SRR4302541.2888261/94‑1 (MQ=60)
                                   GCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAA                                                           <  SRR4302541.847752/94‑1 (MQ=60)
                                    CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          >  SRR4302541.4952792/1‑94 (MQ=60)
                                     GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         >  SRR4302541.4162246/1‑94 (MQ=60)
                                     GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         >  SRR4302541.4162247/1‑94 (MQ=60)
                                     GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         >  SRR4302541.782383/1‑94 (MQ=60)
                                     GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302541.8760889/94‑1 (MQ=60)
                                       ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       >  SRR4302541.2995526/1‑94 (MQ=60)
                                       ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       <  SRR4302541.6279194/94‑1 (MQ=60)
                                       ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       <  SRR4302541.6497013/94‑1 (MQ=60)
                                       ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGAAACAAATCT                                                       >  SRR4302541.2995527/1‑94 (MQ=60)
                                        TGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      >  SRR4302541.2620186/1‑94 (MQ=60)
                                         GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     <  SRR4302541.4763160/94‑1 (MQ=60)
                                         GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     <  SRR4302541.6236863/94‑1 (MQ=60)
                                         GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     >  SRR4302541.2101645/1‑94 (MQ=60)
                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGT                                                    >  SRR4302541.2191831/1‑94 (MQ=60)
                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    <  SRR4302541.7736516/94‑1 (MQ=60)
                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302541.4981022/1‑94 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   <  SRR4302541.5594444/94‑1 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   <  SRR4302541.8219898/94‑1 (MQ=60)
                                            GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  >  SRR4302541.3440638/1‑94 (MQ=60)
                                            GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  <  SRR4302541.4124717/94‑1 (MQ=60)
                                            GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  <  SRR4302541.4124718/94‑1 (MQ=60)
                                            GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  <  SRR4302541.4162328/94‑1 (MQ=60)
                                             GGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTC                                                 >  SRR4302541.4130745/1‑94 (MQ=60)
                                              GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                <  SRR4302541.6009572/94‑1 (MQ=60)
                                              GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                >  SRR4302541.6339004/1‑94 (MQ=60)
                                               TGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               <  SRR4302541.8473244/94‑1 (MQ=60)
                                               TGTTGATGGTGTTCTGGGATCGCTTAAACCGGGAAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               <  SRR4302541.7900936/94‑1 (MQ=60)
                                               GGTTGGTGGTGTTCTGGGAACGGTTAAGACGGGAAAACGCGACAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               <  SRR4302541.7900937/94‑1 (MQ=60)
                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302541.1817604/94‑1 (MQ=60)
                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              >  SRR4302541.8603524/1‑94 (MQ=60)
                                                 TTGATGGTGTTCTGGGATCGCTTAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             <  SRR4302541.8804613/94‑1 (MQ=60)
                                                 TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             <  SRR4302541.4787403/94‑1 (MQ=60)
                                                 TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             >  SRR4302541.6587945/1‑94 (MQ=60)
                                                 TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             <  SRR4302541.7579794/94‑1 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGTTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302541.2873150/94‑1 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCCACAAATCTGGATTCAGGGT                                            >  SRR4302541.8666150/1‑94 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302541.4408242/94‑1 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            >  SRR4302541.2086721/1‑94 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGACACAAATCTGGATTCAGGGC                                            >  SRR4302541.8666349/1‑94 (MQ=60)
                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302541.6607997/1‑94 (MQ=60)
                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           <  SRR4302541.6702447/94‑1 (MQ=60)
                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302541.719498/1‑94 (MQ=60)
                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302541.719497/1‑94 (MQ=60)
                                                    ATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCA                                          >  SRR4302541.8045405/1‑94 (MQ=60)
                                                    ATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCA                                          <  SRR4302541.4530101/94‑1 (MQ=60)
                                                     TGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAA                                         >  SRR4302541.2790444/1‑94 (MQ=60)
                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302541.3696794/94‑1 (MQ=60)
                                                          TTCTGGGATCGCTTAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGC                                    <  SRR4302541.1564788/94‑1 (MQ=60)
                                                          TTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGC                                    >  SRR4302541.8859484/1‑94 (MQ=60)
                                                          TTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGC                                    <  SRR4302541.3384092/94‑1 (MQ=60)
                                                          TTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGC                                    <  SRR4302541.1644623/94‑1 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302541.5877484/94‑1 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302541.8822127/94‑1 (MQ=60)
                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 <  SRR4302541.7817128/94‑1 (MQ=60)
                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 <  SRR4302541.1988127/94‑1 (MQ=60)
                                                             CGGGATCCCTGAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 <  SRR4302541.1988126/94‑1 (MQ=60)
                                                              GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                >  SRR4302541.2047757/1‑94 (MQ=60)
                                                                GATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTT                              >  SRR4302541.1127673/1‑94 (MQ=60)
                                                                GATCCCTTAAACCGGGCAAACGCGCCAGCGTCGTGGCGCCGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTT                              <  SRR4302541.3000302/94‑1 (MQ=60)
                                                                 ATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTT                             >  SRR4302541.1942100/1‑94 (MQ=60)
                                                                 ATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTT                             >  SRR4302541.5734635/1‑94 (MQ=60)
                                                                 ATCGCTTAAACCGGGCAAACGCACCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCACCACATCTGGATTCAGGGTCAATTAGCTTCGTTT                             <  SRR4302541.7881338/94‑1 (MQ=60)
                                                                  TCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            >  SRR4302541.2974056/1‑94 (MQ=60)
                                                                  TCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            <  SRR4302541.4435269/94‑1 (MQ=60)
                                                                  ACGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            <  SRR4302541.5457380/94‑1 (MQ=60)
                                                                   CGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           <  SRR4302541.4890851/94‑1 (MQ=60)
                                                                   CGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           <  SRR4302541.8835702/94‑1 (MQ=60)
                                                                    GCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          >  SRR4302541.1463685/1‑94 (MQ=60)
                                                                    GCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          >  SRR4302541.5163462/1‑94 (MQ=60)
                                                                    GCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          <  SRR4302541.833153/94‑1 (MQ=60)
                                                                     CTTAAACCGGGCAAACGCGCCAGGGTCGTTGCGCTGGATAGCCGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         <  SRR4302541.4495629/94‑1 (MQ=60)
                                                                     CTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         >  SRR4302541.5629536/1‑94 (MQ=60)
                                                                      TTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATA                        <  SRR4302541.5887326/94‑1 (MQ=60)
                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302541.996711/94‑1 (MQ=60)
                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302541.6777102/94‑1 (MQ=60)
                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302541.5803652/94‑1 (MQ=60)
                                                                        AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      >  SRR4302541.4675928/1‑94 (MQ=60)
                                                                        AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      >  SRR4302541.3205482/1‑94 (MQ=60)
                                                                         AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     >  SRR4302541.7010362/1‑94 (MQ=60)
                                                                         AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     <  SRR4302541.6019516/94‑1 (MQ=60)
                                                                         AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     >  SRR4302541.5732036/1‑94 (MQ=60)
                                                                          ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    <  SRR4302541.191304/94‑1 (MQ=60)
                                                                          ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    <  SRR4302541.5201512/94‑1 (MQ=60)
                                                                          ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    <  SRR4302541.5726594/94‑1 (MQ=60)
                                                                           CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   >  SRR4302541.152228/1‑94 (MQ=60)
                                                                            CGGGCAANCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGC                  >  SRR4302541.6237947/1‑94 (MQ=60)
                                                                            CGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGC                  >  SRR4302541.4184225/1‑94 (MQ=60)
                                                                                CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              >  SRR4302541.2896607/1‑94 (MQ=60)
                                                                                 AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAGCAGATCTGGATTCAGGGTTAATTAGCTTCGTTTTGATAGTTTGCTCCTT             <  SRR4302541.5900719/94‑1 (MQ=60)
                                                                                 AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             >  SRR4302541.8347878/1‑94 (MQ=60)
                                                                                 AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             <  SRR4302541.1495551/94‑1 (MQ=60)
                                                                                 AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             >  SRR4302541.4947521/1‑94 (MQ=60)
                                                                                  AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            >  SRR4302541.6637860/1‑94 (MQ=60)
                                                                                  AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            <  SRR4302541.5707948/94‑1 (MQ=60)
                                                                                  AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            >  SRR4302541.3553334/1‑94 (MQ=60)
                                                                                     GCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATT         <  SRR4302541.5346258/94‑1 (MQ=60)
                                                                                     GCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATT         >  SRR4302541.2760469/1‑94 (MQ=60)
                                                                                     GCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATT         >  SRR4302541.523743/1‑94 (MQ=60)
                                                                                     GCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATT         <  SRR4302541.5825830/94‑1 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302541.8069342/94‑1 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        >  SRR4302541.1460624/1‑94 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302541.6612432/94‑1 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTAAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302541.8403882/94‑1 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302541.8039787/1‑94 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302541.2297127/1‑94 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302541.4371267/1‑94 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302541.5743189/1‑94 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       <  SRR4302541.6439775/94‑1 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       <  SRR4302541.6442295/94‑1 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       <  SRR4302541.7499491/94‑1 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302541.7632931/1‑94 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATATGGATTCAGGGTCAATTAGATTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302541.5743439/1‑94 (MQ=60)
                                                                                        CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302541.568436/1‑94 (MQ=60)
                                                                                        CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302541.1651572/1‑94 (MQ=60)
                                                                                        CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGC      >  SRR4302541.1348872/1‑94 (MQ=60)
                                                                                         CAGCGTCGTTGCGCTAGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGC     <  SRR4302541.7471553/94‑1 (MQ=60)
                                                                                          AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCT    >  SRR4302541.8722035/1‑94 (MQ=60)
                                                                                          AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    >  SRR4302541.6325258/1‑94 (MQ=60)
                                                                                          AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    <  SRR4302541.5069006/94‑1 (MQ=60)
                                                                                          AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    >  SRR4302541.3830147/1‑94 (MQ=60)
                                                                                            CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  SRR4302541.6261676/1‑94 (MQ=60)
                                                                                            CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  SRR4302541.2254068/1‑94 (MQ=60)
                                                                                            |                                                                                             
GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279105‑3279290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑