Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,554,118 +C coding (367/396 nt) yhgKJ ← RNA phosphage cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,554,1171.C99.2% 898.6 / ‑2.1 268coding (368/396 nt)yhgKJRNA phosphage cyclase
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (124/142);  total (126/142)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 303 total aligned reads displayed.

TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC  >  NC_000913/3554026‑3554199
                                                                                             |                                                                                  
 ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                                                  <  1:4963481/93‑2 (MQ=255)
   tttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgc                                                                                >  1:5661454/1‑90 (MQ=255)
   tttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgc                                                                                >  2:7395790/1‑90 (MQ=255)
    ttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgct                                                                               >  1:2153995/1‑89 (MQ=255)
      gCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCTGCGGATAAAATCCGCAGGCGGCGCCGACGGGTGATCTGT‑GCCGCCGCTCa                                                                              >  2:5387494/1‑93 (MQ=255)
      gCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCa                                                                              <  2:4558154/93‑1 (MQ=255)
       cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCAc                                                                             <  1:8500144/93‑1 (MQ=255)
         gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                                           <  1:3888065/93‑1 (MQ=255)
           tgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCa                                                                         >  2:8478721/1‑93 (MQ=255)
            gaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCaa                                                                        >  2:7761845/1‑93 (MQ=255)
             aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                                                       <  2:3275996/93‑1 (MQ=255)
             aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                                                       >  2:4685155/1‑93 (MQ=255)
              aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                                                      >  2:6891479/1‑93 (MQ=255)
                 ccTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAacacac                                                                   >  1:3387619/1‑91 (MQ=255)
                 ccTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgc                                                                   >  1:8120036/1‑93 (MQ=255)
                 ccTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgc                                                                   <  2:1719285/93‑1 (MQ=255)
                  cTATTTTCGCCAGCAGCGGCTCCAGCCCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgcg                                                                  <  2:6525025/93‑1 (MQ=255)
                   tATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGa                                                                 >  2:623714/1‑93 (MQ=255)
                   tATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGa                                                                 <  2:7101898/93‑1 (MQ=255)
                     ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGCAg                                                               >  1:6097849/1‑93 (MQ=255)
                      tttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAgg                                                              <  1:6440841/93‑1 (MQ=255)
                        tCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTc                                                            <  1:4669455/93‑1 (MQ=255)
                          gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                                          >  2:6814897/1‑93 (MQ=255)
                           ccaccaGCGGCTCCAGCGCCCGGCGGATAAAGTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                         <  2:4961724/93‑1 (MQ=255)
                           ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                         >  1:4864892/1‑93 (MQ=255)
                           ccAGCAGCGGCCCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                         <  1:6310807/93‑1 (MQ=255)
                            cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                                                        <  1:6032488/93‑1 (MQ=255)
                             agcagcGGCTCCAGCACCCGGCGGAAAAAATCCGCAGGCGGCGCCGACGGGTTATCGG‑AGCCGCCGCTAAATTCCACACGCGAAAGCGCATCg                                                       >  2:6133536/1‑93 (MQ=255)
                              gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                      >  1:8351707/1‑93 (MQ=255)
                              gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                      >  2:7847827/1‑93 (MQ=255)
                              gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                      >  1:6345286/1‑93 (MQ=255)
                              gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                      >  1:1922544/1‑93 (MQ=255)
                              gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGG‑CGCCGCCGCTCCCTTCCACACGCCACGGTCCATCgg                                                      >  1:6209952/1‑93 (MQ=255)
                               cagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGc                                                     >  2:418381/1‑93 (MQ=255)
                                 gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaa                                                   >  2:1973746/1‑93 (MQ=255)
                                   ggCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAc                                                 >  1:1275698/1‑93 (MQ=255)
                                     cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                                               <  1:8413317/93‑1 (MQ=255)
                                      tCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGGCCATCGGCAAACcac                                              >  1:7713485/1‑93 (MQ=255)
                                       ccAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcaca                                             <  1:1123817/93‑1 (MQ=255)
                                       ccAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcaca                                             <  1:2611992/93‑1 (MQ=255)
                                        cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                                            <  1:7416766/93‑1 (MQ=255)
                                        cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                                            >  2:7261877/1‑93 (MQ=255)
                                         aGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                                           >  1:1574834/1‑93 (MQ=255)
                                         aGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACAACAgc                                           >  2:2401287/1‑93 (MQ=255)
                                          gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcg                                          <  2:2368381/93‑1 (MQ=255)
                                          gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcg                                          >  1:4011752/1‑93 (MQ=255)
                                           cACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcgc                                         <  2:2919875/93‑1 (MQ=255)
                                           cACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcgc                                         <  2:7492288/93‑1 (MQ=255)
                                            aCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGcgcg                                        <  2:7542557/93‑1 (MQ=255)
                                            aCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGcgcg                                        >  1:4510733/1‑93 (MQ=255)
                                            aCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGcgcg                                        >  1:7997224/1‑93 (MQ=255)
                                             cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCCCAGCGCgg                                       >  2:1309734/1‑93 (MQ=255)
                                             cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg                                       <  2:6045801/93‑1 (MQ=255)
                                             cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg                                       >  1:6797457/1‑93 (MQ=255)
                                             cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg                                       >  1:442373/1‑93 (MQ=255)
                                              ccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGGCCATCGGCAAACCACAGCGCggg                                      >  2:6310840/1‑93 (MQ=255)
                                               cggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgc                                     <  1:1048613/93‑1 (MQ=255)
                                               cggcggATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgc                                     >  1:5234472/1‑93 (MQ=255)
                                                 gcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcag                                   >  1:3187067/1‑93 (MQ=255)
                                                   ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca                                 >  2:5779600/1‑93 (MQ=255)
                                                    gATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAc                                <  1:379499/93‑1 (MQ=255)
                                                    gATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAc                                <  2:3900920/93‑1 (MQ=255)
                                                     aTAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAcc                               >  1:912450/1‑93 (MQ=255)
                                                      tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg                              >  1:6967180/1‑93 (MQ=255)
                                                        aaaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGtt                            >  1:506733/1‑93 (MQ=255)
                                                        aaaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTAACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGtt                            <  2:5290677/93‑1 (MQ=255)
                                                         aaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGttt                           <  2:1093667/93‑1 (MQ=255)
                                                         aaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGAACCGttt                           >  2:6393700/1‑93 (MQ=255)
                                                            ccgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgca                        <  2:4827631/89‑1 (MQ=255)
                                                            ccgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgca                        <  2:1195072/89‑1 (MQ=255)
                                                             cgcaGGCGGCGCCGACGGGTTATCGGTGCCGG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTGTgcag                       >  1:1624743/4‑93 (MQ=255)
                                                             cgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcag                       >  1:2497063/4‑93 (MQ=255)
                                                             cgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcag                       <  2:2724186/90‑1 (MQ=255)
                                                             cgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcag                       >  1:7872144/4‑93 (MQ=255)
                                                               caGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcagca                     >  1:4124674/2‑93 (MQ=255)
                                                               caGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcagca                     <  2:2314062/92‑1 (MQ=255)
                                                                 ggcggcGCGGACGTGTTATCGGT‑GCCGCCGCCTACCTGAACACGCGAAGGGCCAGCGGCAAACCACAGCGCGGGCAGCACCGGTTGCAGCAcc                   <  1:6567914/93‑1 (MQ=255)
                                                                 ggcggcGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAcc                   >  1:8648277/1‑93 (MQ=255)
                                                                    ggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGc                >  1:4334748/1‑93 (MQ=255)
                                                                    ggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGc                <  2:184467/93‑1 (MQ=255)
                                                                     gcgcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCg               <  2:1688256/93‑1 (MQ=255)
                                                                      ggcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGt              <  2:7939579/92‑1 (MQ=255)
                                                                       gcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTa             <  2:933996/93‑1 (MQ=255)
                                                                       gcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTa             <  2:6604041/93‑1 (MQ=255)
                                                                       gcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTa             <  2:3611572/93‑1 (MQ=255)
                                                                        ccGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTAc            <  2:3408281/93‑1 (MQ=255)
                                                                         cGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACa           <  2:5666861/93‑1 (MQ=255)
                                                                          gACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACaa          <  2:1487418/93‑1 (MQ=255)
                                                                          gACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACaa          >  1:5929478/1‑93 (MQ=255)
                                                                          gACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACaa          <  2:8195728/93‑1 (MQ=255)
                                                                           aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAAc         <  1:5521625/93‑1 (MQ=255)
                                                                           aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAAc         <  2:564415/93‑1 (MQ=255)
                                                                           aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAAc         <  2:8313414/93‑1 (MQ=255)
                                                                             gggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACtt       >  2:4456690/1‑93 (MQ=255)
                                                                               gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     <  1:3557715/93‑1 (MQ=255)
                                                                               gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     <  1:2390983/93‑1 (MQ=255)
                                                                               gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     <  1:1464426/93‑1 (MQ=255)
                                                                               gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     <  2:8537814/93‑1 (MQ=255)
                                                                                  aTCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGgc  >  1:6632743/1‑93 (MQ=255)
                                                                                  aTCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGgc  >  2:3643687/1‑93 (MQ=255)
                                                                                             |                                                                                  
TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC  >  NC_000913/3554026‑3554199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AG‑GCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGC  >  NC_000913/3554025‑3554211
                                                                                                  |                                                                                             
GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCG‑C                                                                                               >  SRR4302541.5886150/1‑94 (MQ=60)
   GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGTGCCGCCG‑C                                                                                               <  SRR4302541.5426131/94‑1 (MQ=60)
   GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGTGCCGCCG‑C                                                                                               >  SRR4302541.5719270/1‑94 (MQ=60)
      TGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCA                                                                                          <  SRR4302541.3334262/94‑1 (MQ=60)
        CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                                                        <  SRR4302541.768999/94‑1 (MQ=60)
        CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                                                        <  SRR4302541.8591685/94‑1 (MQ=60)
        CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                                                        >  SRR4302541.4402512/1‑94 (MQ=60)
        CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                                                        <  SRR4302541.8389874/94‑1 (MQ=60)
          GATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTC                                                                                      >  SRR4302541.2746938/1‑94 (MQ=60)
          GATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTC                                                                                      <  SRR4302541.3927060/94‑1 (MQ=60)
           ATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCA                                                                                     <  SRR4302541.5025584/94‑1 (MQ=60)
           AGGAATTCCTTTGTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCA                                                                                     <  SRR4302541.2280484/94‑1 (MQ=60)
            TGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAA                                                                                    >  SRR4302541.1632032/1‑94 (MQ=60)
             TAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAAC                                                                                   <  SRR4302541.8036132/94‑1 (MQ=60)
             GAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAAC                                                                                   <  SRR4302541.7307253/94‑1 (MQ=60)
              AATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACA                                                                                  <  SRR4302541.2048127/94‑1 (MQ=60)
                TTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACG                                                                                <  SRR4302541.2384304/94‑1 (MQ=60)
                 TCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGC                                                                               >  SRR4302541.8208969/1‑94 (MQ=60)
                 TCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACAC                                                                               >  SRR4302541.3421406/1‑94 (MQ=60)
                 TCCTATTGTCGCCAGCAGCGGCTCCAGCGCCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGC                                                                               <  SRR4302541.1766464/94‑1 (MQ=60)
                  CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑GCCGG‑CGCTCACTTCAACACGCG                                                                              >  SRR4302541.2465246/1‑94 (MQ=60)
                   CTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGA                                                                             >  SRR4302541.2622589/1‑94 (MQ=60)
                     ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGCAG                                                                           >  SRR4302541.6160105/1‑94 (MQ=60)
                      TTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGG                                                                          <  SRR4302541.1682060/94‑1 (MQ=60)
                      TTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGG                                                                          <  SRR4302541.4969111/94‑1 (MQ=60)
                       TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGT                                                                         <  SRR4302541.6506486/94‑1 (MQ=60)
                         TCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCC                                                                       <  SRR4302541.5216162/94‑1 (MQ=60)
                         TCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCC                                                                       <  SRR4302541.6129199/94‑1 (MQ=60)
                         TCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCC                                                                       <  SRR4302541.4717013/94‑1 (MQ=60)
                          CGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCA                                                                      >  SRR4302541.3885144/1‑94 (MQ=60)
                           GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAT                                                                     >  SRR4302541.4914519/1‑94 (MQ=60)
                            CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                                                    <  SRR4302541.5859890/94‑1 (MQ=60)
                            CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                                                    <  SRR4302541.3325325/94‑1 (MQ=60)
                            CCAGCAGCGGCCCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                                                    <  SRR4302541.6375326/94‑1 (MQ=60)
                             CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   >  SRR4302541.3446227/1‑94 (MQ=60)
                             CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   >  SRR4302541.780140/1‑94 (MQ=60)
                             CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   <  SRR4302541.6094262/94‑1 (MQ=60)
                             CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   <  SRR4302541.1530468/94‑1 (MQ=60)
                             CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   <  SRR4302541.145490/94‑1 (MQ=60)
                              caggatcgtccccagcaccctgcggacaaaattcgcagcCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   <  SRR4302541.1243769/55‑1 (MQ=57)
                              AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GGGGCGCCGACGGGTTATCGGTGCCGCCG‑CG‑‑CACTTCCACACGCGAAGGTACCTCGG                                                                  >  SRR4302541.4404296/1‑94 (MQ=57)
                              AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                                                  >  SRR4302541.8442340/1‑94 (MQ=60)
                              AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                                                  >  SRR4302541.1941051/1‑94 (MQ=60)
                              AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                                                  >  SRR4302541.6410157/1‑94 (MQ=60)
                              AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGC                                                                  >  SRR4302541.6273413/1‑94 (MQ=60)
                              AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGG‑‑‑CGCCGCCGCTCCCTTCCACACGCCACGGTCCATCGG                                                                  >  SRR4302541.6273414/1‑94 (MQ=57)
                                 CGCGGCTTCAGCACCCGGGGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAA                                                               <  SRR4302541.6742904/94‑1 (MQ=60)
                                 AGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAA                                                               >  SRR4302541.4134462/1‑94 (MQ=60)
                                 AGCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAA                                                               >  SRR4302541.258191/1‑94 (MQ=60)
                                  GCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAA                                                              <  SRR4302541.1917911/94‑1 (MQ=60)
                                  GCGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAA                                                              <  SRR4302541.3886203/94‑1 (MQ=60)
                                   CGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAC                                                             >  SRR4302541.1288371/1‑94 (MQ=60)
                                   CGGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAC                                                             >  SRR4302541.8807125/1‑94 (MQ=60)
                                    GGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                            >  SRR4302541.5265935/1‑94 (MQ=60)
                                    GGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                            >  SRR4302541.345418/1‑94 (MQ=60)
                                    GGCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                            >  SRR4302541.6404544/1‑94 (MQ=60)
                                     GCTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCA                                                           >  SRR4302541.454366/1‑94 (MQ=60)
                                      GTCCAGCACCCGGCGGATAAAATCCG‑GGTGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                                                          <  SRR4302541.2417747/94‑1 (MQ=60)
                                      CTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                                                          >  SRR4302541.3188209/1‑94 (MQ=60)
                                      CTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                                                          <  SRR4302541.8504294/94‑1 (MQ=60)
                                      CTCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGGCCATCGGCAAACCAC                                                          >  SRR4302541.7795529/1‑94 (MQ=60)
                                       TCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                                                         <  SRR4302541.5385058/94‑1 (MQ=60)
                                       TCCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGATCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                                                         <  SRR4302541.1322045/94‑1 (MQ=60)
                                        CCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCATAG                                                        <  SRR4302541.1134406/94‑1 (MQ=60)
                                        CCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                                                        <  SRR4302541.2638098/94‑1 (MQ=60)
                                        CCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                                                        <  SRR4302541.6397248/94‑1 (MQ=60)
                                        CCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                                                        >  SRR4302541.740189/1‑94 (MQ=60)
                                        CCAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                                                        <  SRR4302541.1134407/94‑1 (MQ=60)
                                         CAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       <  SRR4302541.570517/94‑1 (MQ=60)
                                         CAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       >  SRR4302541.1590581/1‑94 (MQ=60)
                                         CAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       <  SRR4302541.7496076/94‑1 (MQ=60)
                                         CAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       >  SRR4302541.6149121/1‑94 (MQ=60)
                                         CAGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       <  SRR4302541.4922981/94‑1 (MQ=60)
                                          AGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                                                      <  SRR4302541.1150812/94‑1 (MQ=60)
                                          AGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                                                      >  SRR4302541.4051432/1‑94 (MQ=60)
                                          AGCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                                                      >  SRR4302541.8063010/1‑94 (MQ=60)
                                           GCACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGGTATCGGT‑‑‑GCAGCCGCTCACTTAAACACGCGAAGGTCCATCGGAAAACCACCGCGC                                                     >  SRR4302541.6921304/1‑94 (MQ=46)
                                            CACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCG                                                    >  SRR4302541.4556245/1‑94 (MQ=60)
                                            CACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCG                                                    <  SRR4302541.4741254/94‑1 (MQ=60)
                                            CACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCG                                                    >  SRR4302541.8085236/1‑94 (MQ=60)
                                             ACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                                                   >  SRR4302541.446530/1‑94 (MQ=60)
                                             ACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                                                   >  SRR4302541.6870155/1‑94 (MQ=60)
                                             ACCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                                                   >  SRR4302541.7698373/1‑94 (MQ=60)
                                              CCCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                                                  >  SRR4302541.6987716/1‑94 (MQ=60)
                                               CCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                                                 >  SRR4302541.6690248/1‑94 (MQ=60)
                                               CCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                                                 <  SRR4302541.6499562/94‑1 (MQ=60)
                                               CCGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGGTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                                                 >  SRR4302541.5288399/1‑94 (MQ=60)
                                                CGGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCA                                                <  SRR4302541.1058352/94‑1 (MQ=60)
                                                 GGCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAG                                               >  SRR4302541.3219263/1‑94 (MQ=60)
                                                  GCGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGC                                              <  SRR4302541.1637936/94‑1 (MQ=60)
                                                   CGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA                                             >  SRR4302541.811843/1‑94 (MQ=60)
                                                   CGGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA                                             >  SRR4302541.6282787/1‑94 (MQ=60)
                                                    GGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC                                            >  SRR4302541.5670784/1‑94 (MQ=60)
                                                    GGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC                                            <  SRR4302541.6268156/94‑1 (MQ=60)
                                                    GGATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC                                            >  SRR4302541.8456428/1‑94 (MQ=60)
                                                    CGATAAAATCCGCAG‑GCGGCGCCGACGTGTTATCCGT‑‑‑GCCGCCGCTCACTCCAACACGCGAAGGTCCAGCGGCAAACCACAGCGCGGGCAGCAC                                            <  SRR4302541.6430953/94‑1 (MQ=55)
                                                     GATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACC                                           >  SRR4302541.920960/1‑94 (MQ=60)
                                                     GATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACC                                           <  SRR4302541.382994/94‑1 (MQ=60)
                                                      ATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCG                                          >  SRR4302541.59876/1‑94 (MQ=60)
                                                      ATAAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCG                                          >  SRR4302541.7041441/1‑94 (MQ=60)
                                                        AAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTT                                        >  SRR4302541.511483/1‑94 (MQ=60)
                                                        AAAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAAAGCGCGGGCAGCACCGTT                                        >  SRR4302541.3791599/1‑94 (MQ=60)
                                                         AAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT                                       <  SRR4302541.853175/94‑1 (MQ=60)
                                                         AAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT                                       <  SRR4302541.6307517/94‑1 (MQ=60)
                                                         AAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT                                       >  SRR4302541.780814/1‑94 (MQ=60)
                                                         AAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT                                       >  SRR4302541.6827866/1‑94 (MQ=60)
                                                         AAATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACAACAGCGCGGGCAGCACCGTTT                                       <  SRR4302541.8169919/94‑1 (MQ=60)
                                                          AATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTG                                      >  SRR4302541.3843569/1‑94 (MQ=60)
                                                          AATCCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTC                                      >  SRR4302541.6523274/1‑94 (MQ=60)
                                                             CCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑GCCGG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTGTGCAG                                   >  SRR4302541.1640846/1‑94 (MQ=60)
                                                             CCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAG                                   >  SRR4302541.2522271/1‑94 (MQ=60)
                                                             CCGCAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAG                                   >  SRR4302541.7958645/1‑94 (MQ=60)
                                                               gcAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCA                                 >  SRR4302541.4165418/3‑94 (MQ=60)
                                                               gcAG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCA                                 <  SRR4302541.4577962/92‑1 (MQ=60)
                                                                 AG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACG                               >  SRR4302541.4588282/1‑94 (MQ=60)
                                                                 AG‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACC                               >  SRR4302541.8740885/1‑94 (MQ=60)
                                                                  G‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCA                              <  SRR4302541.4287196/94‑1 (MQ=60)
                                                                  G‑GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCA                              >  SRR4302541.8526958/1‑94 (MQ=60)
                                                                    GCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAG                             <  SRR4302541.4019702/94‑1 (MQ=60)
                                                                     CGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGC                            <  SRR4302541.6556967/94‑1 (MQ=60)
                                                                     CGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGC                            >  SRR4302541.4378025/1‑94 (MQ=60)
                                                                      GGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCG                           <  SRR4302541.4130301/94‑1 (MQ=60)
                                                                       GCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGT                          <  SRR4302541.1168306/94‑1 (MQ=60)
                                                                        CGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTA                         >  SRR4302541.6016593/1‑94 (MQ=60)
                                                                         GCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTAC                        <  SRR4302541.4030942/94‑1 (MQ=60)
                                                                          CCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACA                       >  SRR4302541.886272/1‑94 (MQ=60)
                                                                          CCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACA                       >  SRR4302541.4738435/1‑94 (MQ=60)
                                                                           CGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTCCAA                      >  SRR4302541.6419252/1‑94 (MQ=60)
                                                                           CGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAA                      >  SRR4302541.5989871/1‑94 (MQ=60)
                                                                           CGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAA                      >  SRR4302541.6419253/1‑94 (MQ=60)
                                                                            GACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAAC                     >  SRR4302541.6642516/1‑94 (MQ=60)
                                                                             ACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACT                    <  SRR4302541.6572414/94‑1 (MQ=60)
                                                                             ACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACT                    <  SRR4302541.1979441/94‑1 (MQ=60)
                                                                             ACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACT                    <  SRR4302541.5578142/94‑1 (MQ=60)
                                                                              CGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTT                   <  SRR4302541.441055/94‑1 (MQ=60)
                                                                               GGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTC                  >  SRR4302541.338577/1‑94 (MQ=60)
                                                                                GGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCC                 <  SRR4302541.8446672/94‑1 (MQ=60)
                                                                                GGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCC                 >  SRR4302541.3760143/1‑94 (MQ=60)
                                                                                 GTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCG                <  SRR4302541.8628418/94‑1 (MQ=60)
                                                                                 GTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCG                <  SRR4302541.5179496/94‑1 (MQ=60)
                                                                                 GTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCG                <  SRR4302541.1478764/94‑1 (MQ=60)
                                                                                 GTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCG                <  SRR4302541.1478765/94‑1 (MQ=60)
                                                                                 GTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCG                <  SRR4302541.2414381/94‑1 (MQ=60)
                                                                                 GTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCG                <  SRR4302541.3593712/94‑1 (MQ=60)
                                                                                  TTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGG               <  SRR4302541.4517056/94‑1 (MQ=60)
                                                                                  TTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGG               <  SRR4302541.4351137/94‑1 (MQ=60)
                                                                                   TATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC              >  SRR4302541.6700745/1‑94 (MQ=60)
                                                                                   TATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC              <  SRR4302541.8738569/94‑1 (MQ=60)
                                                                                   TATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC              <  SRR4302541.1081871/94‑1 (MQ=60)
                                                                                    ATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCG             >  SRR4302541.424114/1‑94 (MQ=60)
                                                                                        tcggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGC            >  SRR4302541.371127/11‑94 (MQ=60)
                                                                                        tcggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGC            >  SRR4302541.3893959/11‑94 (MQ=60)
                                                                                         cggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCT           <  SRR4302541.5856075/85‑1 (MQ=60)
                                                                                         cggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCT           >  SRR4302541.4118539/10‑94 (MQ=60)
                                                                                          ggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTA          >  SRR4302541.1061961/9‑94 (MQ=60)
                                                                                          ggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTA          >  SRR4302541.4191144/9‑94 (MQ=60)
                                                                                           gtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTAC         >  SRR4302541.2063915/8‑94 (MQ=60)
                                                                                           gtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTAC         <  SRR4302541.152702/87‑1 (MQ=60)
                                                                                            tgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACC        <  SRR4302541.1503427/88‑1 (MQ=60)
                                                                                             GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCG       <  SRR4302541.2021065/94‑1 (MQ=60)
                                                                                             GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCG       <  SRR4302541.7421534/94‑1 (MQ=60)
                                                                                             GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCG       <  SRR4302541.5687933/94‑1 (MQ=60)
                                                                                             GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCG       <  SRR4302541.5951149/94‑1 (MQ=60)
                                                                                              CCGCCGCTCACTTCAACCCGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGA      <  SRR4302541.4058341/94‑1 (MQ=60)
                                                                                              CCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGA      <  SRR4302541.114959/94‑1 (MQ=60)
                                                                                              CCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGA      >  SRR4302541.2576854/1‑94 (MQ=60)
                                                                                               cgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGAT     >  SRR4302541.249852/4‑94 (MQ=60)
                                                                                               cgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGAT     <  SRR4302541.1104751/91‑1 (MQ=60)
                                                                                                gcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATA    <  SRR4302541.1137861/92‑1 (MQ=60)
                                                                                                  CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGC  >  SRR4302541.4042289/1‑94 (MQ=60)
                                                                                                  CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGC  >  SRR4302541.2557932/1‑94 (MQ=60)
                                                                                                  CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCCGCGCTACAGATAGC  >  SRR4302541.8737813/1‑94 (MQ=60)
                                                                                                  |                                                                                             
GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AG‑GCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGC  >  NC_000913/3554025‑3554211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑