Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,007,907 +GG intergenic (+109/‑11) pldB → / → b4555 lysophospholipase L(2)/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,007,9071.G92.6% 1875.5 / 130.0 579intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (23/20);  new base G (256/279);  total (279/300)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01
*NC_0009134,007,9072.G92.6% 1854.8 / 130.0 579intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (23/20);  new base G (256/279);  total (279/300)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-01

Only 100 of 625 total aligned reads displayed.

GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996
                                                                                               ||                                                                                         
gttgttGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgcgc                                                                                             >  1:8385909/1‑93 (MQ=255)
gttgttGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgcgc                                                                                             >  2:1619612/1‑93 (MQ=255)
gttgttGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgcgc                                                                                             >  2:7386157/1‑93 (MQ=255)
gttgttGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgcgc                                                                                             <  2:4197443/93‑1 (MQ=255)
  tgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgcgcgg                                                                                           <  2:3747792/93‑3 (MQ=255)
  tgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgcgcgg                                                                                           <  2:5426041/93‑3 (MQ=255)
    ttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgcgcggca                                                                                         <  1:4019283/93‑5 (MQ=255)
    ttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATACCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cgcgggcggca                                                                                         >  2:5317762/1‑89 (MQ=255)
           tGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACtt                                                                                  >  2:5500364/1‑93 (MQ=255)
             aTTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTtg                                                                                <  1:1011489/93‑1 (MQ=255)
              tttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTtgt                                                                               <  1:2605031/93‑1 (MQ=255)
              tttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTtgt                                                                               <  2:8152604/93‑1 (MQ=255)
                 aGATGGCGCGTTACTTTCTCTCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGtt                                                                            <  2:7021859/93‑1 (MQ=255)
                 aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGtt                                                                            >  2:1529165/1‑93 (MQ=255)
                  gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGttt                                                                           >  1:1348135/1‑93 (MQ=255)
                  gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGttt                                                                           >  1:7759627/1‑93 (MQ=255)
                    tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGc                                                                         <  2:3233289/93‑1 (MQ=255)
                    tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGc                                                                         <  2:8790846/93‑1 (MQ=255)
                        aCGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGAcc                                                                     >  2:8355481/1‑93 (MQ=255)
                         cGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCg                                                                    <  1:2463946/93‑1 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  <  2:3075359/93‑1 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  >  2:6463319/1‑93 (MQ=255)
                             tcTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcg                                                                <  1:7329643/92‑1 (MQ=255)
                              cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                                               <  1:3392760/93‑1 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              <  2:1352000/93‑1 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              >  1:6166076/1‑93 (MQ=255)
                                   tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacca                                                          <  1:8061239/93‑1 (MQ=255)
                                   tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATAAACTTTGTGTTTGCGACCGGTCGTcaccc                                                          >  1:2748392/1‑92 (MQ=255)
                                    cccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcaccac                                                         >  2:4575490/1‑93 (MQ=255)
                                      cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGt                                                       <  1:966024/93‑1 (MQ=255)
                                        aCCATACGTTATCCCCTTACGCCAAAGAAGCTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTg                                                     <  2:2768799/93‑1 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    >  1:4922987/1‑93 (MQ=255)
                                          cATCCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTCTGCGACCGGTCGTCACCACGTTGAt                                                   <  2:6508531/93‑1 (MQ=255)
                                          cATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                                   >  2:4103000/1‑93 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 <  1:1669674/93‑1 (MQ=255)
                                             aCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                                                <  1:4437590/93‑1 (MQ=255)
                                               gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              <  1:3833992/93‑1 (MQ=255)
                                               gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              <  1:6270518/93‑1 (MQ=255)
                                               cttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              <  1:1834632/92‑1 (MQ=255)
                                                  aTCCCCTGACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGGTGATGTGGGGCa                                           >  2:860159/1‑93 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         <  2:8417860/93‑1 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         <  2:6076998/93‑1 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCAGCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         >  1:4340705/1‑93 (MQ=255)
                                                     cccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt                                        <  1:3265994/93‑1 (MQ=255)
                                                     cccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATAAACTTTGTGTTTGCGACCGTTCGTCACCACGTTGATGTGGGGCAAAt                                        >  1:3133050/1‑93 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       <  2:4697637/93‑1 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       <  2:3486527/93‑1 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       >  1:2921082/1‑93 (MQ=255)
                                                       cTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAGATTc                                      >  1:7561434/1‑93 (MQ=255)
                                                       cTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc                                      <  1:3085971/93‑1 (MQ=255)
                                                        ttACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcg                                     >  1:4615924/1‑93 (MQ=255)
                                                         tACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc                                    >  2:2479675/1‑93 (MQ=255)
                                                         tACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc                                    >  2:7772787/1‑93 (MQ=255)
                                                            gCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt                                 <  1:6502601/93‑1 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGCTa                                >  1:3663250/1‑93 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                <  2:7240640/93‑1 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                <  1:4082065/93‑1 (MQ=255)
                                                              cAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATaa                               <  2:7946363/93‑1 (MQ=255)
                                                               aaaGCAACTCTGAAGCTGCGCAC‑‑TGCGCGCTGCATCAGCTTTGTGTTTCAGACCGGACGTCACCACGTTGAAGTGGGGTAAATTCGCGATagt                              >  2:5087814/1‑91 (MQ=255)
                                                               aaaGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCACATTCGCGATAAt                              >  1:7874401/1‑93 (MQ=255)
                                                               aaaGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt                              >  1:4225316/1‑93 (MQ=255)
                                                                acgAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATc                             <  1:6839566/91‑1 (MQ=255)
                                                                aaGAAACTCTCAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATc                             <  1:8498011/93‑1 (MQ=255)
                                                                 aGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCt                            >  2:4468458/1‑93 (MQ=255)
                                                                 aGAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCt                            <  2:5627498/93‑1 (MQ=255)
                                                                  gAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTg                           >  1:5949026/1‑93 (MQ=255)
                                                                  gAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTg                           >  2:1047394/1‑93 (MQ=255)
                                                                  gAAACTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTg                           >  1:5581596/1‑93 (MQ=255)
                                                                    aaCTCTGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGga                         <  1:3459565/93‑1 (MQ=255)
                                                                      ctctGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga                       >  1:3722363/1‑93 (MQ=255)
                                                                      ctctGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga                       <  1:6441385/93‑1 (MQ=255)
                                                                      ctctGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga                       >  1:6953407/1‑93 (MQ=255)
                                                                      ctctGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga                       <  1:8213431/93‑1 (MQ=255)
                                                                       tctGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt                      <  2:6722846/93‑1 (MQ=255)
                                                                         tGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa                    >  2:6356553/1‑93 (MQ=255)
                                                                         tGAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa                    >  1:2329729/1‑93 (MQ=255)
                                                                          gAAGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTaa                   <  1:8691869/93‑1 (MQ=255)
                                                                           aaGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAg                  <  1:1172437/93‑1 (MQ=255)
                                                                            aGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 <  1:8774253/93‑1 (MQ=255)
                                                                            aGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 <  2:2144042/93‑1 (MQ=255)
                                                                            aGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 <  2:257316/93‑1 (MQ=255)
                                                                            aGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 >  1:6645910/1‑93 (MQ=255)
                                                                            aGCTGCTCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 >  2:7679276/1‑93 (MQ=255)
                                                                             gctgctCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTc                <  1:5238976/93‑1 (MQ=255)
                                                                              ctgctCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCt               <  2:4002840/93‑1 (MQ=255)
                                                                               tgctCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCtt              >  1:5042610/1‑93 (MQ=255)
                                                                                gctCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTa             >  2:4970700/1‑93 (MQ=255)
                                                                                gctCAC‑‑CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTa             >  1:5329444/1‑93 (MQ=255)
                                                                                    cccgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         <  1:207734/89‑1 (MQ=255)
                                                                                    accgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGGGATTAAGTCTTACATg         >  1:57602/5‑93 (MQ=255)
                                                                                    accgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         >  2:1268089/5‑93 (MQ=255)
                                                                                    accgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         >  1:7078986/5‑93 (MQ=255)
                                                                                     ccgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGa        >  2:3892053/4‑93 (MQ=255)
                                                                                      cgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAt       <  1:1877675/91‑1 (MQ=255)
                                                                                      cgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAt       <  1:5086501/91‑1 (MQ=255)
                                                                                       gcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAtt      <  1:396042/92‑1 (MQ=255)
                                                                                          cgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAcc   <  2:2256997/93‑1 (MQ=255)
                                                                                          cgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAcc   <  1:19732/93‑1 (MQ=255)
                                                                                          cgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGAGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAcc   <  2:5865301/93‑1 (MQ=255)
                                                                                           gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCt  <  2:1928218/93‑1 (MQ=255)
                                                                                               ||                                                                                         
GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 378 total aligned reads displayed.
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000
                                                                                                   |                                                                                               
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGC                                                                                                 >  SRR4302541.8476717/1‑94 (MQ=60)
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGC                                                                                                 <  SRR4302541.7247845/94‑1 (MQ=60)
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGC                                                                                                 >  SRR4302541.1745624/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGC‑‑G                                                                                              <  SRR4302541.1978670/94‑1 (MQ=60)
  TTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCgg                                                                                               <  SRR4302541.8635128/94‑3 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCggc                                                                                              >  SRR4302541.3593219/1‑91 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCggc                                                                                              >  SRR4302541.4216133/1‑91 (MQ=60)
       GCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCA                                                                                          <  SRR4302541.4568610/94‑1 (MQ=60)
       GCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCA                                                                                          <  SRR4302541.2546786/94‑1 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCGAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAA                                                                                         >  SRR4302541.7669536/1‑94 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAA                                                                                         >  SRR4302541.8066002/1‑94 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAA                                                                                         >  SRR4302541.1351616/1‑94 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAA                                                                                         <  SRR4302541.3272805/94‑1 (MQ=60)
         GTCTGATTTAGCTGGCACGTACCTTTCTCCCGGCCAAACGTTCTCCCCTTACGCCAAAGAAACTCTGAAGCAACTCACCAC‑‑GCG‑‑CGG‑‑CATCAAC                                                                                        <  SRR4302541.7868034/94‑1 (MQ=36)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAAC                                                                                        >  SRR4302541.3035966/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAAC                                                                                        <  SRR4302541.6176059/94‑1 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAAC                                                                                        >  SRR4302541.7558354/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTAAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCggcatcaat                                                                                        >  SRR4302541.6358801/1‑85 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTT                                                                                     <  SRR4302541.6488105/94‑1 (MQ=60)
             GATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTG                                                                                    <  SRR4302541.8614903/94‑1 (MQ=60)
             GATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTG                                                                                    <  SRR4302541.8052540/94‑1 (MQ=60)
               TTTATATGGCACGTTTCTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTG                                                                                  <  SRR4302541.8221706/94‑1 (MQ=60)
               TTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTG                                                                                  <  SRR4302541.4278431/94‑1 (MQ=60)
               TTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTG                                                                                  <  SRR4302541.821496/94‑1 (MQ=60)
                TTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGT                                                                                 >  SRR4302541.8121538/1‑94 (MQ=60)
                 TAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTT                                                                                >  SRR4302541.6424196/1‑94 (MQ=60)
                 TAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTT                                                                                >  SRR4302541.6275979/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGCAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTT                                                                               >  SRR4302541.8620469/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTT                                                                               >  SRR4302541.1037146/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTT                                                                               >  SRR4302541.7842184/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTT                                                                               >  SRR4302541.7078333/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTT                                                                               >  SRR4302541.2406587/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTT                                                                               >  SRR4302541.1361464/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTT                                                                               <  SRR4302541.2687576/94‑1 (MQ=60)
                   GATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTG                                                                              <  SRR4302541.4021414/94‑1 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGC                                                                             >  SRR4302541.1291573/1‑94 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGC                                                                             >  SRR4302541.1314855/1‑94 (MQ=60)
                      GGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGA                                                                           >  SRR4302541.7042626/1‑94 (MQ=60)
                      GGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGA                                                                           >  SRR4302541.952892/1‑94 (MQ=60)
                       GCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGAC                                                                          >  SRR4302541.8411882/1‑94 (MQ=60)
                       GCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGAC                                                                          <  SRR4302541.5645816/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302541.1782024/1‑94 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302541.3577744/1‑94 (MQ=60)
                         CCGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTTTGTTTGCGACCG                                                                        <  SRR4302541.2842290/94‑1 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCG                                                                        <  SRR4302541.4334792/94‑1 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       >  SRR4302541.2084852/1‑94 (MQ=60)
                           TTTACTTTCTCCCTACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGT                                                                      <  SRR4302541.4041932/94‑1 (MQ=60)
                           GTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGT                                                                      <  SRR4302541.7294984/94‑1 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     >  SRR4302541.1009115/1‑94 (MQ=60)
                              TCTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGT                                                                   <  SRR4302541.7407906/94‑1 (MQ=60)
                               CTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTC                                                                  >  SRR4302541.6229079/1‑94 (MQ=60)
                               CTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTC                                                                  >  SRR4302541.5295360/1‑94 (MQ=60)
                                TTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCA                                                                 <  SRR4302541.6127/94‑1 (MQ=60)
                                 TTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGAGCG‑‑CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCAC                                                                <  SRR4302541.2620691/94‑1 (MQ=60)
                                 TTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCAC                                                                >  SRR4302541.4609181/1‑94 (MQ=60)
                                  TCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACC                                                               <  SRR4302541.8534716/94‑1 (MQ=60)
                                   CTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCA                                                              <  SRR4302541.2373917/94‑1 (MQ=60)
                                    TCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCAC                                                             <  SRR4302541.8149804/94‑1 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            >  SRR4302541.6643710/1‑94 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            >  SRR4302541.6921573/1‑94 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            >  SRR4302541.2514944/1‑94 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            <  SRR4302541.519876/94‑1 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCAGTCGTCACCACG                                                            >  SRR4302541.3353463/1‑94 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTCCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            <  SRR4302541.3135092/94‑1 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          >  SRR4302541.7249108/1‑94 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          <  SRR4302541.1596928/94‑1 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          >  SRR4302541.4534071/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         >  SRR4302541.4393783/1‑94 (MQ=60)
                                         ACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGA                                                        >  SRR4302541.4973216/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302541.4299755/94‑1 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302541.1968608/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302541.5111328/94‑1 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302541.3732035/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302541.6690890/94‑1 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      <  SRR4302541.6247696/94‑1 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     >  SRR4302541.2289241/1‑94 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     <  SRR4302541.3211567/94‑1 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     <  SRR4302541.414672/94‑1 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACGCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     <  SRR4302541.892429/94‑1 (MQ=60)
                                             TACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTG                                                    <  SRR4302541.6318694/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302541.591734/94‑1 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  >  SRR4302541.199343/1‑94 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  <  SRR4302541.6938451/94‑1 (MQ=60)
                                               CGTTATCCCCTCACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  <  SRR4302541.5343707/94‑1 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302541.447961/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302541.5957010/94‑1 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302541.1723423/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302541.1938226/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302541.6334539/94‑1 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302541.8324121/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302541.3872614/94‑1 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302541.3771590/94‑1 (MQ=60)
                                                GTTATCCCCTTACCCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302541.209135/94‑1 (MQ=60)
                                                CTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302541.1852418/94‑1 (MQ=60)
                                                 TTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGC                                                <  SRR4302541.5360767/94‑1 (MQ=60)
                                                 TTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGC                                                >  SRR4302541.4108072/1‑94 (MQ=60)
                                                 TTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGC                                                <  SRR4302541.2170390/94‑1 (MQ=60)
                                                  TATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCA                                               <  SRR4302541.7702172/94‑1 (MQ=60)
                                                   ATCCCCTTACGCCAAAGAGACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAA                                              <  SRR4302541.2696617/94‑1 (MQ=60)
                                                    TCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAA                                             >  SRR4302541.3426089/1‑94 (MQ=60)
                                                    TCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCAGCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAA                                             >  SRR4302541.4384046/1‑94 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            >  SRR4302541.3760281/1‑94 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            >  SRR4302541.1890998/1‑94 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            >  SRR4302541.1864997/1‑94 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           >  SRR4302541.5119592/1‑94 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           >  SRR4302541.2950453/1‑94 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           <  SRR4302541.3298950/94‑1 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCG‑‑CGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAGATTC                                          >  SRR4302541.7642240/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          <  SRR4302541.973082/94‑1 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          <  SRR4302541.8827431/94‑1 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          <  SRR4302541.373201/94‑1 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         <  SRR4302541.3117290/94‑1 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         <  SRR4302541.5882752/94‑1 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         <  SRR4302541.570954/94‑1 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGGCGTCACCACGTTGATGTGGGGCAAATTCG                                         >  SRR4302541.6664941/1‑94 (MQ=60)
                                                            CGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAT                                     >  SRR4302541.4111660/1‑94 (MQ=60)
                                                            CGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAT                                     >  SRR4302541.5623297/1‑94 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGCTA                                    >  SRR4302541.3700416/1‑94 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATA                                    >  SRR4302541.4687504/1‑94 (MQ=60)
                                                              CCAAAGAATCTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAA                                   <  SRR4302541.4122358/94‑1 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCGC‑‑C‑‑GCG‑‑CGCGGCATCATCTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  <  SRR4302541.2510571/94‑1 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCACATTCGCGATAAT                                  >  SRR4302541.7960923/1‑94 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  <  SRR4302541.6755194/94‑1 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  >  SRR4302541.4034709/1‑94 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCATCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  <  SRR4302541.343085/94‑1 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 >  SRR4302541.6870844/1‑94 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 <  SRR4302541.6532967/94‑1 (MQ=60)
                                                                 AAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                >  SRR4302541.3161274/1‑94 (MQ=60)
                                                                 AAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                >  SRR4302541.8491177/1‑94 (MQ=60)
                                                                 AAGAAACTCTCAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                <  SRR4302541.8589547/94‑1 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTG                               <  SRR4302541.2730928/94‑1 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTG                               <  SRR4302541.3117780/94‑1 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTG                               <  SRR4302541.425700/94‑1 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTG                               >  SRR4302541.6009648/1‑94 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCATCTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              <  SRR4302541.3959621/94‑1 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              <  SRR4302541.3486743/94‑1 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              >  SRR4302541.5097045/1‑94 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              <  SRR4302541.3807237/94‑1 (MQ=60)
                                                                   AAAACACTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCAGCATAAACTTTGTTTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              <  SRR4302541.7882986/94‑1 (MQ=60)
                                                                    AAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGA                             >  SRR4302541.1894455/1‑94 (MQ=60)
                                                                    AAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTCGTGTTTGCGACCGGTCGTCACCACGTTGATGTTGGGCAAATTCGCTATAATCTGGG                             >  SRR4302541.7746283/1‑94 (MQ=60)
                                                                     AACTCTGAAGCTGCTCAC‑‑C‑‑GCGCCCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            <  SRR4302541.7487155/94‑1 (MQ=60)
                                                                     AACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            <  SRR4302541.330465/94‑1 (MQ=60)
                                                                     AACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            <  SRR4302541.3494132/94‑1 (MQ=60)
                                                                      ACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGA                           >  SRR4302541.7027561/1‑94 (MQ=60)
                                                                      ACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGA                           >  SRR4302541.3759878/1‑94 (MQ=60)
                                                                       CTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302541.3001536/94‑1 (MQ=60)
                                                                       CTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302541.8303037/94‑1 (MQ=60)
                                                                        TCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATT                         >  SRR4302541.5634940/1‑94 (MQ=60)
                                                                        TCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATT                         >  SRR4302541.5329901/1‑94 (MQ=60)
                                                                         CTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA                        <  SRR4302541.5053148/94‑1 (MQ=60)
                                                                          TGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       >  SRR4302541.5024870/1‑94 (MQ=60)
                                                                          TGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       >  SRR4302541.2865914/1‑94 (MQ=60)
                                                                          TGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGAATCAACTTTGTGTTTGCGACCGGTCGTCACCACGCTGATCTGGGGCAAATTCGCGATAATCTGGGGATTAG                       >  SRR4302541.2014055/1‑94 (MQ=60)
                                                                          TGAAGCTGCACAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       >  SRR4302541.4116985/1‑94 (MQ=60)
                                                                           acattTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAG                      <  SRR4302541.8798501/89‑1 (MQ=60)
                                                                           GAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAT                      >  SRR4302541.110032/1‑94 (MQ=60)
                                                                           GAAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAG                      <  SRR4302541.8784912/94‑1 (MQ=60)
                                                                            AAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     >  SRR4302541.6714078/1‑94 (MQ=60)
                                                                            AAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     <  SRR4302541.1185143/94‑1 (MQ=60)
                                                                            AAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     <  SRR4302541.2182330/94‑1 (MQ=60)
                                                                            AAGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     >  SRR4302541.4582736/1‑94 (MQ=60)
                                                                             AGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302541.3173006/1‑94 (MQ=60)
                                                                             AGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302541.4470320/1‑94 (MQ=60)
                                                                             AGCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    <  SRR4302541.8868045/94‑1 (MQ=60)
                                                                              GCTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCT                   >  SRR4302541.6555043/1‑94 (MQ=60)
                                                                               CTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTT                  >  SRR4302541.5094501/1‑94 (MQ=60)
                                                                               CTGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTT                  >  SRR4302541.5186876/1‑94 (MQ=60)
                                                                                TGCTCAC‑‑C‑‑GCG‑‑CGCGGCGTCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTA                 <  SRR4302541.7906530/94‑1 (MQ=60)
                                                                                TGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTA                 <  SRR4302541.7416377/94‑1 (MQ=60)
                                                                                TGCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTA                 <  SRR4302541.2782292/94‑1 (MQ=60)
                                                                                 GCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTAC                <  SRR4302541.2454365/94‑1 (MQ=60)
                                                                                 GCTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTAC                <  SRR4302541.1166702/94‑1 (MQ=60)
                                                                                  CTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTGCA               <  SRR4302541.2105966/94‑1 (MQ=60)
                                                                                  CTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302541.6854986/1‑94 (MQ=60)
                                                                                  CTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302541.1253638/1‑94 (MQ=60)
                                                                                  CTCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCAATAATCTGGAGATTAAGTCTTACA               >  SRR4302541.4789787/1‑94 (MQ=60)
                                                                                   TCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              <  SRR4302541.1579474/94‑1 (MQ=60)
                                                                                   TCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              >  SRR4302541.2874562/1‑94 (MQ=60)
                                                                                   TCAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              >  SRR4302541.2639099/1‑94 (MQ=60)
                                                                                    CAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGGGATTAAGTCTTACATG             >  SRR4302541.58061/1‑94 (MQ=60)
                                                                                    CAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             >  SRR4302541.1656257/1‑94 (MQ=60)
                                                                                    CAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             >  SRR4302541.8218962/1‑94 (MQ=60)
                                                                                    CAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             >  SRR4302541.3468293/1‑94 (MQ=60)
                                                                                    CAC‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             >  SRR4302541.7154280/1‑94 (MQ=60)
                                                                                      G‑‑C‑‑GCG‑‑CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTA         <  SRR4302541.399710/94‑1 (MQ=60)
                                                                                      G‑‑C‑‑GCG‑‑CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTA         <  SRR4302541.3906862/94‑1 (MQ=60)
                                                                                      G‑‑C‑‑GCG‑‑CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTA         <  SRR4302541.527973/94‑1 (MQ=60)
                                                                                      C‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           >  SRR4302541.1000030/1‑94 (MQ=60)
                                                                                      C‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           >  SRR4302541.6967872/1‑94 (MQ=60)
                                                                                      C‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           >  SRR4302541.6198903/1‑94 (MQ=60)
                                                                                      C‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           >  SRR4302541.5424467/1‑94 (MQ=60)
                                                                                      C‑‑C‑‑GCG‑‑CGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           <  SRR4302541.4848166/94‑1 (MQ=60)
                                                                                         C‑‑GCG‑‑CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302541.6568449/94‑1 (MQ=60)
                                                                                            GCG‑‑CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACC       <  SRR4302541.3052490/94‑1 (MQ=60)
                                                                                                 CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCC    >  SRR4302541.6640066/1‑94 (MQ=60)
                                                                                                  GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   >  SRR4302541.1425073/1‑94 (MQ=60)
                                                                                                  GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   >  SRR4302541.6466145/1‑94 (MQ=60)
                                                                                                  GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   >  SRR4302541.4287757/1‑94 (MQ=60)
                                                                                                  GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   >  SRR4302541.4675038/1‑94 (MQ=60)
                                                                                                   |                                                                                               
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑C‑‑GCG‑‑CGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑