Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,491 2 bp→CG intergenic (+58/‑174) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4910GC99.8% 1572.2 / NA 460intergenic (+58/‑175)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (209/249);  minor base T (1/0);  total (210/250)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*NC_0009134,008,4920CG99.8% 1552.4 / NA 453intergenic (+59/‑174)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (205/247);  minor base A (0/1);  total (205/248)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 482 total aligned reads displayed.

CTACAGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008405‑4008577
                                                                                             ||                                                                                      
    gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCgcacc                                                                                    >  2:3688305/3‑93 (MQ=255)
      cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAg                                                                                  <  1:6765214/93‑1 (MQ=255)
      cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAg                                                                                  >  2:5479235/1‑93 (MQ=255)
       tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCCCCAGc                                                                                 >  2:7305919/1‑93 (MQ=255)
       tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:7029053/1‑93 (MQ=255)
       tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  1:8512069/1‑93 (MQ=255)
        gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg                                                                                >  2:6328675/1‑93 (MQ=255)
          aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              <  2:820869/93‑1 (MQ=255)
          aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              <  1:8589651/93‑1 (MQ=255)
          aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              >  1:920898/1‑93 (MQ=255)
           ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt                                                                             >  1:4723247/1‑93 (MQ=255)
           ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt                                                                             >  2:1599206/1‑93 (MQ=255)
                        tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                <  1:1064579/89‑1 (MQ=255)
                        tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                <  1:4256310/89‑1 (MQ=255)
                        tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                <  2:5756100/89‑1 (MQ=255)
                         ttggtgGCGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGGCCTTCATc                                                               <  2:4389861/90‑1 (MQ=255)
                          tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              >  2:3517939/3‑93 (MQ=255)
                          tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              <  1:3647842/91‑1 (MQ=255)
                          tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              <  1:7649371/91‑1 (MQ=255)
                          tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              <  1:454380/91‑1 (MQ=255)
                           ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc                                                             <  2:6841789/92‑1 (MQ=255)
                            gtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                                            <  1:609659/93‑1 (MQ=255)
                            gtgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                                            >  1:5810606/1‑93 (MQ=255)
                             tgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCgg                                                           >  2:4773487/1‑92 (MQ=255)
                             tgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa                                                           >  2:2031275/1‑93 (MQ=255)
                             tgACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa                                                           <  2:8502061/93‑1 (MQ=255)
                               gcGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc                                                         <  1:2835311/92‑1 (MQ=255)
                               aCGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCATCAGCGTCTGAAAGACCTTCATCCCGAGc                                                         >  1:7816024/1‑93 (MQ=255)
                               aCGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc                                                         >  1:5554259/1‑93 (MQ=255)
                                 gggATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                      >  2:4298491/1‑93 (MQ=255)
                                  ggATGAACGACGCCGAAATGCTGTCGATGGCC‑GAGAAAGG‑CGGCAGTATCGGCAGTGCGCACCAGCGCCTGAAAGACCGCCATCCCGAGCT‑gg                                                     >  1:1013688/1‑92 (MQ=255)
                                  ggATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAGAGACCTTCATCCCGAGCT‑gg                                                     >  1:179106/1‑92 (MQ=255)
                                   gATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    <  1:4645539/93‑3 (MQ=255)
                                   gATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    >  1:5936163/1‑91 (MQ=255)
                                    aTGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   >  1:3243318/1‑90 (MQ=255)
                                       aaCGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                 <  2:1884988/93‑1 (MQ=255)
                                        acgacgCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                >  1:5203085/1‑93 (MQ=255)
                                        acgacgCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                >  1:3995786/1‑93 (MQ=255)
                                          gacgCCGAAATGCTGTCGATGG‑CGGGGAAA‑GTCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGCGCTGGAAGTGAtg                                              >  1:2534490/1‑92 (MQ=255)
                                           gcgCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             <  1:7331666/92‑1 (MQ=255)
                                           acgCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             <  2:5027477/93‑1 (MQ=255)
                                           acgCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             <  2:7246999/93‑1 (MQ=255)
                                            cgCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                            >  1:5064670/1‑93 (MQ=255)
                                            cgCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                            <  2:7616666/93‑1 (MQ=255)
                                            cgCCGAAAGGCGGTCGACGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTTATTgg                                            <  2:7670775/93‑1 (MQ=255)
                                             gCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                                           >  1:142046/1‑93 (MQ=255)
                                             gCCGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                                           >  2:6090499/1‑93 (MQ=255)
                                              ccGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                          <  2:6760344/93‑1 (MQ=255)
                                               cGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                         <  1:4121373/93‑1 (MQ=255)
                                               cGAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                         <  2:3956311/93‑1 (MQ=255)
                                                gAAATGCTGTCGATGG‑CGGGGAAATG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  2:5146382/1‑93 (MQ=255)
                                                gAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTGCt                                        >  2:3358404/1‑93 (MQ=255)
                                                gAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  1:373523/1‑93 (MQ=255)
                                                gAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  1:4817652/1‑93 (MQ=255)
                                                gAAATGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  1:6323218/1‑93 (MQ=255)
                                                   aTGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     >  2:5617719/1‑93 (MQ=255)
                                                   aTGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     <  2:6463627/93‑1 (MQ=255)
                                                    tGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  1:2324319/1‑93 (MQ=255)
                                                    tGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  1:1165224/1‑93 (MQ=255)
                                                    tGCTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGCCTGTAAGACCTTCATCACGAGCTGGAAGTGATTGGTACTAATg                                    >  2:1259375/1‑93 (MQ=255)
                                                      cTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                  <  1:2203057/93‑1 (MQ=255)
                                                      cTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                  <  2:2860286/93‑1 (MQ=255)
                                                      cTGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                  >  1:2660311/1‑93 (MQ=255)
                                                       tGTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  1:5319807/1‑93 (MQ=255)
                                                        gTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                >  2:8004907/1‑93 (MQ=255)
                                                        gTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  1:157193/93‑1 (MQ=255)
                                                        gTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  2:6012094/93‑1 (MQ=255)
                                                        gTCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                >  1:8345395/1‑93 (MQ=255)
                                                         tCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAGAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               <  2:2512899/93‑1 (MQ=255)
                                                         tCGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               >  2:589820/1‑93 (MQ=255)
                                                          cGATGG‑CGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg                              <  1:5704105/93‑1 (MQ=255)
                                                           gATGG‑CGGGGAAAGG‑CTGCATTATGGGCATTGCGCTCCAGGGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCggacga                             >  1:4910888/1‑93 (MQ=255)
                                                               ggcggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                          >  1:7957703/5‑93 (MQ=255)
                                                                  ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCggg                       >  1:4783818/2‑92 (MQ=255)
                                                                  ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       >  2:3237709/2‑93 (MQ=255)
                                                                  ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       <  2:1743405/92‑1 (MQ=255)
                                                                  ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       <  1:6901924/92‑1 (MQ=255)
                                                                   gggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGCTTGGTACTAATGCCGACGACGCGGTg                      >  1:345811/1‑93 (MQ=255)
                                                                    ggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     >  2:4578087/1‑93 (MQ=255)
                                                                    ggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     <  1:6433305/93‑1 (MQ=255)
                                                                      aaagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCg                   <  2:491295/89‑1 (MQ=255)
                                                                       aagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGCAGc                  >  1:74798/4‑93 (MQ=255)
                                                                        agg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                 <  1:2495127/91‑1 (MQ=255)
                                                                        agg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                 >  2:3790743/3‑93 (MQ=255)
                                                                        agg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                 <  1:6650931/91‑1 (MQ=255)
                                                                         gg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                <  2:6248340/92‑1 (MQ=255)
                                                                          g‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               >  2:1445026/1‑93 (MQ=255)
                                                                          g‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               <  2:7762099/93‑1 (MQ=255)
                                                                          g‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               >  2:6584529/1‑93 (MQ=255)
                                                                          g‑CTGCATTATGGGCAGTGCGCACCAGCGTCAGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               <  2:265851/93‑1 (MQ=255)
                                                                             tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  1:8018173/93‑1 (MQ=255)
                                                                              gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            <  2:5383739/93‑1 (MQ=255)
                                                                               gaTTAGGGGCAGTGCTCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  1:3805033/92‑1 (MQ=255)
                                                                               cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  1:1302241/93‑1 (MQ=255)
                                                                                aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg          <  1:8154783/93‑1 (MQ=255)
                                                                                 ttATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         <  1:1631289/93‑1 (MQ=255)
                                                                                  tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg        <  1:4192549/93‑1 (MQ=255)
                                                                                  tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg        >  2:3040434/1‑93 (MQ=255)
                                                                                    tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTTATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa      >  1:2063939/1‑93 (MQ=255)
                                                                                      ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa    <  1:7064104/93‑1 (MQ=255)
                                                                                             ||                                                                                      
CTACAGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008405‑4008577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 307 total aligned reads displayed.
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑G‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT  >  NC_000913/4008404‑4008583
                                                                                                  |                                                                                             
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGC                                                                                               <  SRR4302541.2683317/94‑1 (MQ=60)
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGC                                                                                               >  SRR4302541.6985101/1‑94 (MQ=60)
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGC                                                                                               <  SRR4302541.5032962/94‑1 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                                          <  SRR4302541.3728099/91‑1 (MQ=60)
      gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCA                                                                                         >  SRR4302541.1034499/3‑94 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAG                                                                                        <  SRR4302541.4946985/94‑1 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAG                                                                                        >  SRR4302541.4684356/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                                       <  SRR4302541.6837562/94‑1 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                                       >  SRR4302541.8603706/1‑94 (MQ=60)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                                                    >  SRR4302541.929521/1‑94 (MQ=57)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                                                    >  SRR4302541.2091259/1‑94 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                                   <  SRR4302541.8681837/94‑1 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                                   <  SRR4302541.22608/94‑1 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                                   >  SRR4302541.4771421/1‑94 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                                   <  SRR4302541.8054256/94‑1 (MQ=57)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                                  >  SRR4302541.7803486/1‑94 (MQ=60)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                                  >  SRR4302541.1812144/1‑94 (MQ=60)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                                  >  SRR4302541.7918976/1‑94 (MQ=60)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                                  <  SRR4302541.1605592/94‑1 (MQ=60)
               ATTGTATTGCGTTTGGTGACTGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAA                                                                                >  SRR4302541.4751753/1‑94 (MQ=53)
               ATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAA                                                                                <  SRR4302541.6772046/94‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                              <  SRR4302541.5546968/81‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                              <  SRR4302541.1518337/81‑1 (MQ=60)
                  T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                                            <  SRR4302541.2459847/94‑1 (MQ=60)
                  T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                                            <  SRR4302541.4912426/94‑1 (MQ=60)
                    ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                                           >  SRR4302541.8356964/1‑94 (MQ=60)
                     TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCT                                                                          <  SRR4302541.4114079/94‑1 (MQ=60)
                     TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCT                                                                          >  SRR4302541.7912744/1‑94 (MQ=60)
                     TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCACACCAGCGTCTGAAAGACCT                                                                          >  SRR4302541.5074237/1‑94 (MQ=60)
                      TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTT                                                                         <  SRR4302541.5038902/94‑1 (MQ=60)
                       GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC                                                                        <  SRR4302541.8872159/94‑1 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                                       <  SRR4302541.7109202/94‑1 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                                       <  SRR4302541.1747605/94‑1 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCCTCTGAAAGACCTTCA                                                                       >  SRR4302541.2029371/1‑94 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                      >  SRR4302541.6385160/6‑94 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                      >  SRR4302541.2134949/6‑94 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                      >  SRR4302541.185911/6‑94 (MQ=60)
                          tttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATC                                                                     <  SRR4302541.4298857/90‑1 (MQ=60)
                          tttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATC                                                                     <  SRR4302541.1074479/90‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                                   <  SRR4302541.6909363/94‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                                   <  SRR4302541.3684840/94‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                                   <  SRR4302541.1895285/94‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                                   <  SRR4302541.458711/94‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                                   <  SRR4302541.6818647/94‑1 (MQ=60)
                           ttgGTGACGGGATGAACGACGCCGGAATGCTGTCGATGG‑C‑GGCG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCGCCAGCGTCTGAAAGACCTTCATCC                                                                    <  SRR4302541.2523257/91‑1 (MQ=60)
                           ttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCCCCAGCGTCTGAAAGGCCTTCATCC                                                                    <  SRR4302541.4581208/91‑1 (MQ=60)
                           ttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCC                                                                    <  SRR4302541.8604777/91‑1 (MQ=60)
                           G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCG                                                                  <  SRR4302541.1900682/94‑1 (MQ=60)
                           G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCG                                                                  >  SRR4302541.5869639/1‑94 (MQ=60)
                           G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCG                                                                  <  SRR4302541.525188/94‑1 (MQ=60)
                              GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                                                 >  SRR4302541.4635404/1‑94 (MQ=60)
                              GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                                                 <  SRR4302541.615391/94‑1 (MQ=60)
                               TGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                                                >  SRR4302541.150676/1‑94 (MQ=60)
                               TGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                                                <  SRR4302541.6196975/94‑1 (MQ=60)
                               TGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                                                <  SRR4302541.590926/94‑1 (MQ=60)
                               GGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                                                <  SRR4302541.2797744/94‑1 (MQ=60)
                                GACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCATCAGCGTCTGAAAGACCTTCATCCCGAGC                                                               >  SRR4302541.7900653/1‑94 (MQ=60)
                                GACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                                               >  SRR4302541.5611111/1‑94 (MQ=60)
                                GACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                                               >  SRR4302541.5611110/1‑94 (MQ=60)
                                 GCGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT                                                              <  SRR4302541.2863854/94‑1 (MQ=60)
                                 ACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT                                                              <  SRR4302541.1578372/94‑1 (MQ=60)
                                  CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                            >  SRR4302541.3133635/1‑94 (MQ=60)
                                  CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                            <  SRR4302541.5750722/94‑1 (MQ=60)
                                  CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                            <  SRR4302541.2331969/94‑1 (MQ=60)
                                   GGGATGAACGACGCCGAAATGCTGTCGATGGCC‑GA‑G‑‑AAAGG‑CGGCAGTATCGGCAGTGCGCACCAGCGCCTGAAAGACCGCCATCCCGAGCT‑GG                                                           >  SRR4302541.1023062/1‑94 (MQ=19)
                                   GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAT‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                           >  SRR4302541.1337932/1‑94 (MQ=60)
                                   GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                           <  SRR4302541.1135414/94‑1 (MQ=60)
                                   GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                           >  SRR4302541.7466181/1‑94 (MQ=60)
                                   GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                           >  SRR4302541.2630159/1‑94 (MQ=60)
                                    GGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                          <  SRR4302541.4773953/94‑1 (MQ=60)
                                    GGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                          >  SRR4302541.5996642/1‑94 (MQ=60)
                                    GGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCT‑GGA                                                          >  SRR4302541.1253601/1‑94 (MQ=60)
                                     GATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                         <  SRR4302541.1739807/94‑4 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGTGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                         >  SRR4302541.6164569/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                         <  SRR4302541.7825424/94‑1 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑ATGCATTATGGGCAGTGCGCACCAGCGCCTGAAAGAACTTCATCCAGAGCT‑Gggag                                                        >  SRR4302541.5163733/1‑90 (MQ=46)
                                        GAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                       >  SRR4302541.1087736/1‑94 (MQ=60)
                                        GAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                       <  SRR4302541.3463599/94‑1 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                      >  SRR4302541.4121483/1‑94 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                      >  SRR4302541.4035350/1‑94 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                      >  SRR4302541.3988307/1‑94 (MQ=60)
                                          ACGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                     <  SRR4302541.3577843/94‑1 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGG‑CGGG‑G‑‑AAA‑GTCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGCGCTGGAAGTGATG                                                    >  SRR4302541.2559944/1‑94 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                    <  SRR4302541.4844192/94‑1 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                    <  SRR4302541.1730346/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                   <  SRR4302541.8346157/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                   <  SRR4302541.8575370/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTG                                                   >  SRR4302541.810265/1‑94 (MQ=60)
                                             ACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                  <  SRR4302541.3703579/94‑1 (MQ=60)
                                             ACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                  >  SRR4302541.5116742/1‑94 (MQ=60)
                                             ACGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                  >  SRR4302541.7686696/1‑94 (MQ=60)
                                              CGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                 >  SRR4302541.2460123/1‑94 (MQ=60)
                                              CGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                 <  SRR4302541.7661731/94‑1 (MQ=60)
                                              CGCCGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                 <  SRR4302541.7060571/94‑1 (MQ=60)
                                                 CGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                              <  SRR4302541.8110775/94‑1 (MQ=60)
                                                 CGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                              >  SRR4302541.4866765/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                              <  SRR4302541.4162074/94‑1 (MQ=60)
                                                 CGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                              >  SRR4302541.6387863/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                              >  SRR4302541.376947/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAGCCCGAGCTGGAAGTGATTGGTACT                                              <  SRR4302541.8110776/94‑1 (MQ=60)
                                                  GAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTTAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                             >  SRR4302541.2884169/1‑94 (MQ=60)
                                                  GAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                             >  SRR4302541.1382218/1‑94 (MQ=60)
                                                  GAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                             <  SRR4302541.5733849/94‑1 (MQ=60)
                                                  GAAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                             >  SRR4302541.7375337/1‑94 (MQ=60)
                                                   AAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                            >  SRR4302541.8028823/1‑94 (MQ=60)
                                                   AAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                            >  SRR4302541.4213203/1‑94 (MQ=60)
                                                   AAATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                            >  SRR4302541.6741639/1‑94 (MQ=60)
                                                    AATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                           >  SRR4302541.6560091/1‑94 (MQ=60)
                                                    AATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                           >  SRR4302541.3112378/1‑94 (MQ=60)
                                                     ATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCCTTTTGGGCAGTGCGCACCAGGGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTCATG                                          >  SRR4302541.1176417/1‑94 (MQ=38)
                                                     ATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                          <  SRR4302541.3781178/94‑1 (MQ=60)
                                                     ATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                          >  SRR4302541.2346873/1‑94 (MQ=60)
                                                     ATGCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                          >  SRR4302541.1176416/1‑94 (MQ=60)
                                                     ATGATGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                          <  SRR4302541.4169162/94‑1 (MQ=60)
                                                       GCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                        >  SRR4302541.2686767/1‑94 (MQ=60)
                                                       GCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                        <  SRR4302541.6073581/94‑1 (MQ=60)
                                                       GCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                        <  SRR4302541.4794956/94‑1 (MQ=60)
                                                       GCTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                        >  SRR4302541.3851607/1‑94 (MQ=60)
                                                        CTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                       <  SRR4302541.5277916/94‑1 (MQ=60)
                                                        CTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                       >  SRR4302541.5374565/1‑94 (MQ=60)
                                                        CTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                       <  SRR4302541.5466535/94‑1 (MQ=60)
                                                        CTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                       >  SRR4302541.4708960/1‑94 (MQ=60)
                                                        CTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                       <  SRR4302541.751246/94‑1 (MQ=60)
                                                        CTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                       <  SRR4302541.2224400/94‑1 (MQ=60)
                                                        CTGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                       <  SRR4302541.8020705/94‑1 (MQ=60)
                                                         TGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                      >  SRR4302541.4747699/1‑94 (MQ=60)
                                                         TGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                      <  SRR4302541.2518187/94‑1 (MQ=60)
                                                         TGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                      >  SRR4302541.7338821/1‑94 (MQ=60)
                                                         TGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                      >  SRR4302541.8435980/1‑94 (MQ=60)
                                                         TGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                      <  SRR4302541.3398874/94‑1 (MQ=60)
                                                         TGTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                      <  SRR4302541.4792544/94‑1 (MQ=60)
                                                          GTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                     <  SRR4302541.158371/94‑1 (MQ=60)
                                                          GTCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                     <  SRR4302541.7486067/94‑1 (MQ=60)
                                                           TCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                    <  SRR4302541.2253927/94‑1 (MQ=60)
                                                           TCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                    >  SRR4302541.4209091/1‑94 (MQ=60)
                                                           TCGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCAGAGCTGGAAGAGATTGGTACTAATGCCGACG                                    >  SRR4302541.2519927/1‑94 (MQ=57)
                                                            CGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                   <  SRR4302541.3737417/94‑1 (MQ=60)
                                                            CGATGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                   <  SRR4302541.5762265/94‑1 (MQ=60)
                                                               TGG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTCATGCCGACGACGC                                >  SRR4302541.8045630/1‑94 (MQ=60)
                                                                GG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                               <  SRR4302541.7072929/94‑1 (MQ=60)
                                                                GG‑C‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                               <  SRR4302541.5185355/94‑1 (MQ=60)
                                                                     GG‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                           >  SRR4302541.8063340/1‑94 (MQ=60)
                                                                      cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGG                             >  SRR4302541.4832652/10‑94 (MQ=60)
                                                                      G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                          <  SRR4302541.3389081/94‑1 (MQ=60)
                                                                      G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                          >  SRR4302541.792080/1‑94 (MQ=60)
                                                                      G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                          <  SRR4302541.492899/94‑1 (MQ=60)
                                                                       ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                            >  SRR4302541.6450623/9‑94 (MQ=60)
                                                                       ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                            >  SRR4302541.3791372/9‑94 (MQ=60)
                                                                       ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                            <  SRR4302541.6975569/86‑1 (MQ=60)
                                                                        G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                         >  SRR4302541.3378176/1‑94 (MQ=60)
                                                                        G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                         <  SRR4302541.8779656/94‑1 (MQ=60)
                                                                        G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                         >  SRR4302541.4084572/1‑94 (MQ=60)
                                                                        G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                         <  SRR4302541.7679120/94‑1 (MQ=60)
                                                                        G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                         <  SRR4302541.7576863/94‑1 (MQ=60)
                                                                        G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                         >  SRR4302541.8609003/1‑94 (MQ=60)
                                                                        G‑‑AAAGG‑CTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                         <  SRR4302541.3267401/94‑1 (MQ=60)
                                                                           AAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                       <  SRR4302541.3540161/94‑1 (MQ=60)
                                                                           AAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                       >  SRR4302541.8758197/1‑94 (MQ=60)
                                                                           AAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCAGCA                       >  SRR4302541.6910491/1‑94 (MQ=60)
                                                                           AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                        >  SRR4302541.4249704/1‑94 (MQ=60)
                                                                           AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGCAGC                        >  SRR4302541.75432/1‑94 (MQ=60)
                                                                            AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                      >  SRR4302541.5783626/1‑94 (MQ=60)
                                                                            AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                      <  SRR4302541.6415677/94‑1 (MQ=60)
                                                                            AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                      >  SRR4302541.1661807/1‑94 (MQ=60)
                                                                            AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                      >  SRR4302541.5259304/1‑94 (MQ=60)
                                                                             G‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                     <  SRR4302541.8441938/94‑1 (MQ=60)
                                                                                 CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                   <  SRR4302541.434258/94‑1 (MQ=60)
                                                                                 CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                   <  SRR4302541.2758230/94‑1 (MQ=60)
                                                                                  TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                  >  SRR4302541.2622984/1‑94 (MQ=60)
                                                                                  TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                  >  SRR4302541.5518223/1‑94 (MQ=60)
                                                                                   GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                 <  SRR4302541.2182467/94‑1 (MQ=60)
                                                                                    GATTAGGGGCAGTGCTCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                <  SRR4302541.3843339/94‑1 (MQ=60)
                                                                                    CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                <  SRR4302541.5849008/94‑1 (MQ=60)
                                                                                    CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                <  SRR4302541.1315080/94‑1 (MQ=60)
                                                                                    CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                <  SRR4302541.5086549/94‑1 (MQ=60)
                                                                                     ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATatcttcg               >  SRR4302541.4714643/1‑87 (MQ=60)
                                                                                     ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC               <  SRR4302541.8243910/94‑1 (MQ=60)
                                                                                     ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC               >  SRR4302541.6620522/1‑94 (MQ=60)
                                                                                     ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC               <  SRR4302541.2550874/94‑1 (MQ=60)
                                                                                     ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC               <  SRR4302541.3802315/94‑1 (MQ=60)
                                                                                     ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC               <  SRR4302541.5112738/94‑1 (MQ=60)
                                                                                     ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC               <  SRR4302541.4342255/94‑1 (MQ=60)
                                                                                      TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG              <  SRR4302541.1420527/94‑1 (MQ=60)
                                                                                       TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT             >  SRR4302541.8567613/1‑94 (MQ=60)
                                                                                        ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGACGACGACGCGGTGCCGCATTATCTGCGTA            <  SRR4302541.1024269/94‑1 (MQ=60)
                                                                                        ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCANCCCGAGCTGGAAGTGATTGGTACTAATGCCGANGANNNGGNGCCGCATNNTNNNCNTA            >  SRR4302541.288693/1‑94 (MQ=60)
                                                                                         TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA           >  SRR4302541.7319384/1‑94 (MQ=60)
                                                                                         TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA           <  SRR4302541.1599591/94‑1 (MQ=60)
                                                                                         TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA           <  SRR4302541.6016003/94‑1 (MQ=60)
                                                                                          GGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA          <  SRR4302541.4948649/94‑1 (MQ=60)
                                                                                          GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTTCTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA          >  SRR4302541.6668725/1‑94 (MQ=60)
                                                                                          GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA          >  SRR4302541.4250709/1‑94 (MQ=60)
                                                                                          GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA          <  SRR4302541.7773386/94‑1 (MQ=60)
                                                                                          GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAACCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA          <  SRR4302541.2728445/94‑1 (MQ=60)
                                                                                           GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC         <  SRR4302541.7139237/94‑1 (MQ=60)
                                                                                           GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC         <  SRR4302541.1528729/94‑1 (MQ=60)
                                                                                           GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC         <  SRR4302541.5914894/94‑1 (MQ=60)
                                                                                            GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT        <  SRR4302541.91049/94‑1 (MQ=60)
                                                                                             CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC       >  SRR4302541.5872007/1‑94 (MQ=60)
                                                                                             CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC       <  SRR4302541.1263114/94‑1 (MQ=60)
                                                                                                  |                                                                                             
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GG‑G‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT  >  NC_000913/4008404‑4008583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑