Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,013,772 +C intergenic (‑53/‑246) ysgA ← / → udp hypothetical protein/uridine phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,013,7701.C99.0% 1661.0 / 4.7 485intergenic (‑51/‑248)ysgA/udphypothetical protein/uridine phosphorylase
Reads supporting (aligned to +/- strand):  ref base . (0/5);  new base C (220/258);  total (222/263)
Fisher's exact test for biased strand distribution p-value = 6.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 544 total aligned reads displayed.

GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG  >  NC_000913/4013679‑4013857
                                                                                             |                                                                                       
gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTa                                                                                          >  1:4825358/1‑93 (MQ=255)
 cgtcgtCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑c                                                                                        <  2:4801141/93‑1 (MQ=255)
  gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                                                                       >  2:8196578/1‑93 (MQ=255)
   tcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccc                                                                                      <  1:30614/93‑2 (MQ=255)
    cgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccct                                                                                     <  2:831837/93‑3 (MQ=255)
    cgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccct                                                                                     <  1:32100/93‑3 (MQ=255)
     gtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctt                                                                                    <  2:5025409/93‑4 (MQ=255)
     gtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctt                                                                                    >  2:566245/1‑90 (MQ=255)
     gtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctt                                                                                    <  2:2035161/93‑4 (MQ=255)
      tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                                                                                   >  1:460185/1‑89 (MQ=255)
      tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                                                                                   >  2:3041789/1‑89 (MQ=255)
      tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                                                                                   >  2:3983845/1‑89 (MQ=255)
      tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                                                                                   >  1:2698931/1‑89 (MQ=255)
        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                                                                                  >  1:7190781/1‑93 (MQ=255)
         ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaa                                                                                 >  1:5688706/1‑93 (MQ=255)
          gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaa                                                                                >  2:7500558/1‑93 (MQ=255)
          gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaa                                                                                >  1:1751182/1‑93 (MQ=255)
            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                                                                              <  2:8038448/93‑1 (MQ=255)
            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                                                                              <  1:6355756/93‑1 (MQ=255)
            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                                                                              >  2:4514674/1‑93 (MQ=255)
              cTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATg                                                                            <  1:1516726/93‑1 (MQ=255)
               tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGt                                                                           >  2:6953790/1‑93 (MQ=255)
               tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGt                                                                           <  1:6561000/93‑1 (MQ=255)
                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                         >  1:1149467/1‑93 (MQ=255)
                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                         <  1:4407630/93‑1 (MQ=255)
                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                         >  2:8788579/1‑93 (MQ=255)
                  aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                                                                        >  1:4266788/1‑93 (MQ=255)
                  aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                                                                        <  1:675206/93‑1 (MQ=255)
                  aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                                                                        >  2:1815933/1‑93 (MQ=255)
                   cGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgcg                                                                       <  2:5110206/93‑1 (MQ=255)
                    gATGGTCGAAGCGAGAGTCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgcgc                                                                      <  2:8509420/93‑1 (MQ=255)
                     aTGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCa                                                                     <  1:5125149/93‑1 (MQ=255)
                         tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGt                                                                 <  1:5957091/93‑1 (MQ=255)
                          cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGtt                                                                <  1:2250844/93‑1 (MQ=255)
                           gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTa                                                               <  2:1621729/93‑1 (MQ=255)
                            aaGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTaa                                                              >  1:2973160/1‑93 (MQ=255)
                            aaGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTaa                                                              >  2:6805916/1‑93 (MQ=255)
                              gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACt                                                            >  1:2339304/1‑93 (MQ=255)
                               cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta                                                           <  2:5229672/93‑1 (MQ=255)
                               cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta                                                           <  1:8108072/93‑1 (MQ=255)
                               cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta                                                           <  2:952795/93‑1 (MQ=255)
                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtata                                                         <  1:5204172/93‑1 (MQ=255)
                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtata                                                         <  1:6046368/93‑1 (MQ=255)
                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      <  2:2900985/89‑1 (MQ=255)
                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      >  2:5937768/5‑93 (MQ=255)
                                     gcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCa                                                     <  1:8538267/90‑1 (MQ=255)
                                      cgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAg                                                    <  1:2549678/91‑1 (MQ=255)
                                      cgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAg                                                    >  2:6484257/3‑93 (MQ=255)
                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                   <  1:3802399/92‑1 (MQ=255)
                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                   >  2:3864973/2‑93 (MQ=255)
                                        aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCa                                                  >  2:1571077/1‑93 (MQ=255)
                                         tGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAt                                                 <  2:7728377/93‑1 (MQ=255)
                                             gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                             >  2:4227637/1‑93 (MQ=255)
                                             gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                             <  1:8663274/93‑1 (MQ=255)
                                              cagGTGCAAATCCAGATTGCTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaac                                            >  2:4074148/1‑93 (MQ=255)
                                               agGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaaca                                           <  1:3705490/93‑1 (MQ=255)
                                                ggTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaacaa                                          <  2:2328029/93‑1 (MQ=255)
                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACaaa                                         >  1:1000372/1‑93 (MQ=255)
                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCTTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATc                                       <  2:5598190/93‑1 (MQ=255)
                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATc                                       <  1:1946143/93‑1 (MQ=255)
                                                     aaaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcac                                     <  2:6309452/93‑1 (MQ=255)
                                                     aaaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcac                                     <  1:3366362/93‑1 (MQ=255)
                                                      aaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                    <  1:758060/93‑1 (MQ=255)
                                                       aTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAt                                   <  2:102397/93‑1 (MQ=255)
                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  <  1:7089565/93‑1 (MQ=255)
                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  >  1:1788630/1‑93 (MQ=255)
                                                          cAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGc                                <  1:5432600/93‑1 (MQ=255)
                                                           aGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGcc                               >  1:7855801/1‑93 (MQ=255)
                                                            gATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCt                              >  2:8706037/1‑93 (MQ=255)
                                                              ttgttgAGATTTTGTTATGGAATTCTTTGTATCCTAATAAAACTGTTGCGCAATGCTCTATATAGTCAGCATGCAACATATCACAATGctaca                            >  2:585758/1‑89 (MQ=255)
                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATc                         >  1:207999/1‑93 (MQ=255)
                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATc                         >  1:8417451/1‑93 (MQ=255)
                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATc                         <  1:6863880/93‑1 (MQ=255)
                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATc                         <  2:6053266/93‑1 (MQ=255)
                                                                   gtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCgg                       <  2:3483222/93‑1 (MQ=255)
                                                                    tgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGc                      <  1:4031271/93‑1 (MQ=255)
                                                                    tgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGc                      >  1:5517895/1‑93 (MQ=255)
                                                                     gttgttGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCt                     >  2:7407107/1‑93 (MQ=255)
                                                                      ttgttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTc                    <  2:7134449/93‑1 (MQ=255)
                                                                        gttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAt                  <  1:917961/93‑1 (MQ=255)
                                                                        gttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAt                  >  2:6198269/1‑93 (MQ=255)
                                                                          tgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCt                <  1:6244283/93‑1 (MQ=255)
                                                                           gCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtt               >  1:7698020/1‑93 (MQ=255)
                                                                           gCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtt               <  1:776621/93‑1 (MQ=255)
                                                                             cATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtttt             >  2:6698563/1‑93 (MQ=255)
                                                                              aTGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa            <  1:1581015/93‑1 (MQ=255)
                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           >  1:4491103/1‑93 (MQ=255)
                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           >  2:2495574/1‑93 (MQ=255)
                                                                                ggTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg          >  2:2619706/1‑93 (MQ=255)
                                                                                ggTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg          >  1:4999641/1‑93 (MQ=255)
                                                                                ggTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg          <  2:3445795/93‑1 (MQ=255)
                                                                                 gTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGc         <  2:7285194/93‑1 (MQ=255)
                                                                                  ttttCTCCGTAGCCGTATAAAAATGGTGCGCAATGGTAACTATAGTCAGCATTCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa        <  1:389875/91‑1 (MQ=255)
                                                                                  tATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa        <  2:1209260/93‑1 (MQ=255)
                                                                                   aTTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAg       >  2:2245096/1‑93 (MQ=255)
                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt      <  2:5352004/93‑1 (MQ=255)
                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt      >  2:7812396/1‑93 (MQ=255)
                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt      >  2:2789781/1‑93 (MQ=255)
                                                                                     tctcCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTc     <  2:134200/93‑1 (MQ=255)
                                                                                       tcCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCt   <  1:5820439/93‑1 (MQ=255)
                                                                                             |                                                                                       
GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG  >  NC_000913/4013679‑4013857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 323 total aligned reads displayed.
CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013680‑4013865
                                                                                               |                                                                                             
 GTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCC                                                                                              >  SRR4302541.2187800/1‑94 (MQ=60)
 GTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCC                                                                                              >  SRR4302541.3658625/1‑94 (MQ=60)
 GTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCC                                                                                              <  SRR4302541.561223/94‑1 (MQ=60)
  TCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCCT                                                                                             <  SRR4302541.30858/94‑1 (MQ=60)
   CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCctt                                                                                            <  SRR4302541.4846642/94‑4 (MQ=60)
   CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCctt                                                                                            <  SRR4302541.8357641/94‑4 (MQ=60)
    GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTA                                                                                            >  SRR4302541.5404673/1‑94 (MQ=60)
    GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTA                                                                                            <  SRR4302541.3259844/94‑1 (MQ=60)
    GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTA                                                                                            <  SRR4302541.4641566/94‑1 (MQ=60)
    GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTA                                                                                            >  SRR4302541.7048459/1‑94 (MQ=60)
     TCCGGGGTCTGAACGATTGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                                                                           <  SRR4302541.75155/94‑1 (MQ=60)
     TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                                                                           >  SRR4302541.21320/1‑94 (MQ=60)
     TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                                                                           >  SRR4302541.635979/1‑94 (MQ=60)
     GCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                                                                           <  SRR4302541.2340284/94‑1 (MQ=60)
      CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                                                                          <  SRR4302541.7416358/94‑1 (MQ=60)
      CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                                                                          >  SRR4302541.7267483/1‑94 (MQ=60)
       CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAA                                                                                         >  SRR4302541.5746743/1‑94 (MQ=60)
       CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAA                                                                                         >  SRR4302541.7172886/1‑94 (MQ=60)
       CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAA                                                                                         <  SRR4302541.7793364/94‑1 (MQ=60)
       CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAA                                                                                         >  SRR4302541.102756/1‑94 (MQ=60)
       CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAA                                                                                         >  SRR4302541.5970635/1‑94 (MQ=60)
        GGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAA                                                                                        >  SRR4302541.1768263/1‑94 (MQ=60)
        GGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAA                                                                                        <  SRR4302541.6832761/94‑1 (MQ=60)
         GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAA                                                                                       >  SRR4302541.2095033/1‑94 (MQ=60)
         GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAA                                                                                       >  SRR4302541.5220521/1‑94 (MQ=60)
           GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAAT                                                                                     <  SRR4302541.2300395/94‑1 (MQ=60)
           GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAAT                                                                                     <  SRR4302541.6941755/94‑1 (MQ=60)
           GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAAT                                                                                     <  SRR4302541.6420735/94‑1 (MQ=60)
           GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAAT                                                                                     <  SRR4302541.6420734/94‑1 (MQ=60)
           GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAAT                                                                                     <  SRR4302541.2910682/94‑1 (MQ=60)
             CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGT                                                                                   <  SRR4302541.1531858/94‑1 (MQ=60)
             CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGT                                                                                   <  SRR4302541.1531860/94‑1 (MQ=60)
              TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                                                                                  >  SRR4302541.7226527/1‑94 (MQ=60)
              TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                                                                                  >  SRR4302541.4970959/1‑94 (MQ=60)
               taacggtgggCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTG                                                                                 <  SRR4302541.4059568/84‑1 (MQ=60)
               GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTG                                                                                 >  SRR4302541.6108199/1‑94 (MQ=60)
               GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTG                                                                                 >  SRR4302541.1160428/1‑94 (MQ=60)
               GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTG                                                                                 >  SRR4302541.1673291/1‑94 (MQ=60)
                AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATTGTATTCTCCGTGC‑CCTTATAAAAATGTTGC                                                                                <  SRR4302541.874232/94‑1 (MQ=60)
                AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                                                                                >  SRR4302541.5712210/1‑94 (MQ=60)
                AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                                                                                <  SRR4302541.3304498/94‑1 (MQ=60)
                AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                                                                                >  SRR4302541.4309431/1‑94 (MQ=60)
                AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                                                                                >  SRR4302541.3966160/1‑94 (MQ=60)
                 ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               <  SRR4302541.4540459/94‑1 (MQ=60)
                 ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               <  SRR4302541.4891761/94‑1 (MQ=60)
                 ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               <  SRR4302541.681578/94‑1 (MQ=60)
                 ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                                                                               <  SRR4302541.3761031/94‑1 (MQ=60)
                   GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCA                                                                             >  SRR4302541.2418983/1‑94 (MQ=60)
                      GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATG                                                                          <  SRR4302541.2866833/94‑1 (MQ=60)
                      GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATG                                                                          >  SRR4302541.1792643/1‑94 (MQ=60)
                        TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302541.3024012/94‑1 (MQ=60)
                        TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302541.6017806/94‑1 (MQ=60)
                        TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302541.7454485/94‑1 (MQ=60)
                         CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       <  SRR4302541.8768070/94‑1 (MQ=60)
                         CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       >  SRR4302541.4494974/1‑94 (MQ=60)
                         CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       <  SRR4302541.2272595/94‑1 (MQ=60)
                         CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA                                                                       >  SRR4302541.2208031/1‑94 (MQ=60)
                          GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAA                                                                      >  SRR4302541.4376377/1‑94 (MQ=60)
                          GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAA                                                                      >  SRR4302541.3003124/1‑94 (MQ=60)
                          GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAA                                                                      <  SRR4302541.1886300/94‑1 (MQ=60)
                            AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACT                                                                    >  SRR4302541.1972097/1‑94 (MQ=60)
                            AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACT                                                                    <  SRR4302541.6195653/94‑1 (MQ=60)
                            AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACT                                                                    <  SRR4302541.4566578/94‑1 (MQ=60)
                             accagaggcgatgcagcatgggcaaatccaggtgctgttggtggTGCCATTGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTA                                                                   <  SRR4302541.2041375/50‑1 (MQ=51)
                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302541.417491/94‑1 (MQ=60)
                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTAT                                                                  >  SRR4302541.1589205/1‑94 (MQ=60)
                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302541.8196923/94‑1 (MQ=60)
                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302541.5617983/94‑1 (MQ=60)
                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302541.5816163/94‑1 (MQ=60)
                               cagaggcgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATA                                                                 <  SRR4302541.8145845/86‑1 (MQ=60)
                               GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATA                                                                 <  SRR4302541.5212399/94‑1 (MQ=60)
                               GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATA                                                                 <  SRR4302541.3776660/94‑1 (MQ=60)
                                AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTTCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                >  SRR4302541.6021815/1‑94 (MQ=60)
                                AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                <  SRR4302541.5257773/94‑1 (MQ=60)
                                 GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT                                                               >  SRR4302541.5396482/1‑94 (MQ=60)
                                  aggcgcTGCAGCAGGTGCAAATCCAGCTTGATGTGCTGTTGTCATTGTATTCTCCGT‑ACCCTTATAAAAAGGTTGCGCAATGTTAACTATAGTC                                                              <  SRR4302541.2544438/88‑1 (MQ=58)
                                  AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATACAAATGTTGCGCAATGTTAACTATAGTC                                                              >  SRR4302541.251798/1‑94 (MQ=60)
                                  AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              >  SRR4302541.7814318/1‑94 (MQ=60)
                                  AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              >  SRR4302541.3232787/1‑94 (MQ=60)
                                   GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302541.5802498/94‑1 (MQ=60)
                                   GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302541.5540316/94‑1 (MQ=60)
                                    gcgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAG                                                            <  SRR4302541.936307/91‑1 (MQ=60)
                                    gcgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAG                                                            <  SRR4302541.1282371/91‑1 (MQ=60)
                                    gcgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAG                                                            <  SRR4302541.8630072/91‑1 (MQ=60)
                                     cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           <  SRR4302541.2575251/92‑1 (MQ=60)
                                     cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGA                                                           >  SRR4302541.5016218/3‑94 (MQ=60)
                                     G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA                                                          >  SRR4302541.1843439/1‑94 (MQ=60)
                                     G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA                                                          <  SRR4302541.3840670/94‑1 (MQ=60)
                                       ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAT                                                         <  SRR4302541.3197179/94‑1 (MQ=60)
                                        TGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATG                                                        <  SRR4302541.6614081/94‑1 (MQ=60)
                                           AGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAA                                                     >  SRR4302541.3156532/1‑94 (MQ=60)
                                            GCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAAC                                                    <  SRR4302541.8755986/94‑1 (MQ=60)
                                             CAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACA                                                   >  SRR4302541.5986093/1‑94 (MQ=60)
                                              AGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTTCGCAATGTTAACTATAGTCAGCATGCCACAA                                                  >  SRR4302541.1721625/1‑94 (MQ=60)
                                              AGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAGCAA                                                  >  SRR4302541.3803053/1‑94 (MQ=60)
                                              AGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAA                                                  <  SRR4302541.2654456/94‑1 (MQ=60)
                                               GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 >  SRR4302541.1009674/1‑94 (MQ=60)
                                               GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 <  SRR4302541.8430617/94‑1 (MQ=60)
                                               GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 >  SRR4302541.5922048/1‑94 (MQ=60)
                                               GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 <  SRR4302541.2669755/94‑1 (MQ=60)
                                                GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                <  SRR4302541.3505632/94‑1 (MQ=60)
                                                GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                >  SRR4302541.1005848/1‑94 (MQ=60)
                                                 TGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATC                                               <  SRR4302541.466534/94‑1 (MQ=60)
                                                 TGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATC                                               >  SRR4302541.3928024/1‑94 (MQ=60)
                                                  GCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCA                                              >  SRR4302541.6306385/1‑94 (MQ=60)
                                                  GCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCA                                              <  SRR4302541.1964897/94‑1 (MQ=60)
                                                  GCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCGTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCA                                              <  SRR4302541.6918072/94‑1 (MQ=60)
                                                   CAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCAC                                             <  SRR4302541.8195842/94‑1 (MQ=60)
                                                    AAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACA                                            >  SRR4302541.8615060/1‑94 (MQ=60)
                                                    AAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACA                                            <  SRR4302541.7784680/94‑1 (MQ=60)
                                                     AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           <  SRR4302541.73617/94‑1 (MQ=60)
                                                     AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           >  SRR4302541.664805/1‑94 (MQ=60)
                                                      ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          >  SRR4302541.1806007/1‑94 (MQ=60)
                                                        CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        >  SRR4302541.6564875/1‑94 (MQ=60)
                                                        CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        <  SRR4302541.241023/94‑1 (MQ=60)
                                                         CAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCC                                       >  SRR4302541.2946321/1‑94 (MQ=60)
                                                         CAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCC                                       <  SRR4302541.5488330/94‑1 (MQ=60)
                                                         CAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCC                                       >  SRR4302541.7942053/1‑94 (MQ=60)
                                                          AGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCT                                      <  SRR4302541.7092464/94‑1 (MQ=60)
                                                          AGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCT                                      >  SRR4302541.1773602/1‑94 (MQ=60)
                                                          AGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCT                                      <  SRR4302541.6062217/94‑1 (MQ=60)
                                                           GATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     <  SRR4302541.7849135/94‑1 (MQ=60)
                                                           GATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     <  SRR4302541.4648312/94‑1 (MQ=60)
                                                             TTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAA                                   <  SRR4302541.4804667/94‑1 (MQ=60)
                                                             TTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAA                                   <  SRR4302541.6174980/94‑1 (MQ=60)
                                                             TTGTTGTGTTGTTGCCATGGTATGCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAA                                   <  SRR4302541.2024762/94‑1 (MQ=60)
                                                               GTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATC                                 <  SRR4302541.8053851/94‑1 (MQ=60)
                                                               GTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATC                                 <  SRR4302541.1587572/94‑1 (MQ=60)
                                                               GTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATC                                 >  SRR4302541.8508458/1‑94 (MQ=60)
                                                                TTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                >  SRR4302541.6782058/1‑94 (MQ=60)
                                                                TTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302541.6937210/94‑1 (MQ=60)
                                                                TTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302541.1637006/94‑1 (MQ=60)
                                                                TTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302541.4358783/94‑1 (MQ=60)
                                                                TTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302541.4452930/94‑1 (MQ=60)
                                                                TTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                >  SRR4302541.5054708/1‑94 (MQ=60)
                                                                TTGTGTTGGTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302541.850555/94‑1 (MQ=60)
                                                                 TGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGG                               >  SRR4302541.5329231/1‑94 (MQ=60)
                                                                  GTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGC                              >  SRR4302541.4331168/1‑94 (MQ=60)
                                                                  GTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGC                              >  SRR4302541.7704177/1‑94 (MQ=60)
                                                                  GTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGC                              >  SRR4302541.5574377/1‑94 (MQ=60)
                                                                    tttttttccattGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTC                            <  SRR4302541.7693963/82‑1 (MQ=60)
                                                                     TTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCA                           >  SRR4302541.8915224/1‑94 (MQ=60)
                                                                     TTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCA                           <  SRR4302541.6156371/94‑1 (MQ=60)
                                                                      TGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAT                          <  SRR4302541.3049652/94‑1 (MQ=60)
                                                                       GTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATC                         <  SRR4302541.7767378/94‑1 (MQ=60)
                                                                        TTGCCATGGTATTCTCCGT‑ATCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAATAAATCACATTGCTTGAATCGGCTCATCT                        >  SRR4302541.7873590/1‑94 (MQ=60)
                                                                           CCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTT                     <  SRR4302541.5383250/94‑1 (MQ=60)
                                                                           CCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTT                     >  SRR4302541.5470495/1‑94 (MQ=60)
                                                                           CCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTT                     >  SRR4302541.5538775/1‑94 (MQ=60)
                                                                           CCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTT                     >  SRR4302541.4009903/1‑94 (MQ=60)
                                                                            CATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    >  SRR4302541.8667212/1‑94 (MQ=60)
                                                                             ATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAAGAAATCACATTGACTGAATCGGCTCATCTTTTAT                   >  SRR4302541.4215103/1‑94 (MQ=60)
                                                                             ATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   <  SRR4302541.3690325/94‑1 (MQ=60)
                                                                              TGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATG                  >  SRR4302541.1209553/1‑94 (MQ=60)
                                                                               GGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGC                 <  SRR4302541.449121/94‑1 (MQ=60)
                                                                               GGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGC                 >  SRR4302541.7756108/1‑94 (MQ=60)
                                                                               GGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGC                 <  SRR4302541.3861400/94‑1 (MQ=60)
                                                                                 TTTTCTCCGT‑AGCCGTATAAAAATGGTGCGCAATGGTAACTATAGTCAGCATTCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAG               <  SRR4302541.393478/94‑1 (MQ=60)
                                                                                 TATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTTCCTGAATCGGCTCATCTTTTATGCAG               <  SRR4302541.8750589/94‑1 (MQ=60)
                                                                                 TATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAG               <  SRR4302541.5156788/94‑1 (MQ=60)
                                                                                 TATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAG               <  SRR4302541.6363462/94‑1 (MQ=60)
                                                                                   TTCTCCGT‑ACCCTTATCAACATGTTGCGCAATGTTAACTATAGTCTGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAATC             <  SRR4302541.7888345/94‑1 (MQ=60)
                                                                                    TCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCC            <  SRR4302541.6355736/94‑1 (MQ=60)
                                                                                     CTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           >  SRR4302541.1787369/1‑94 (MQ=60)
                                                                                     CTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302541.3456991/94‑1 (MQ=60)
                                                                                     CTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302541.930971/94‑1 (MQ=60)
                                                                                     CTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302541.716057/94‑1 (MQ=60)
                                                                                      TCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG          <  SRR4302541.5115280/94‑1 (MQ=60)
                                                                                      TCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG          <  SRR4302541.5879599/94‑1 (MQ=60)
                                                                                        CGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        >  SRR4302541.1282165/1‑94 (MQ=60)
                                                                                        CGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        >  SRR4302541.4321529/1‑94 (MQ=60)
                                                                                        CGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        >  SRR4302541.32767/1‑94 (MQ=60)
                                                                                        CGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        >  SRR4302541.2340996/1‑94 (MQ=60)
                                                                                        CGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        <  SRR4302541.678117/94‑1 (MQ=60)
                                                                                        CGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        >  SRR4302541.2817286/1‑94 (MQ=60)
                                                                                         GT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACGTTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAG       >  SRR4302541.4308711/1‑94 (MQ=60)
                                                                                         GT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAG       >  SRR4302541.8656218/1‑94 (MQ=60)
                                                                                         GT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAG       <  SRR4302541.5654801/94‑1 (MQ=60)
                                                                                         GT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAG       >  SRR4302541.3883008/1‑94 (MQ=60)
                                                                                           tacCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGA      >  SRR4302541.647287/4‑94 (MQ=60)
                                                                                           tacCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGA      >  SRR4302541.2764648/4‑94 (MQ=60)
                                                                                           tacCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGACCTGCAGA      >  SRR4302541.8839164/4‑94 (MQ=60)
                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     <  SRR4302541.3293542/92‑1 (MQ=60)
                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302541.3109705/3‑94 (MQ=60)
                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302541.1021785/3‑94 (MQ=60)
                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302541.5373407/3‑94 (MQ=60)
                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302541.7574818/3‑94 (MQ=60)
                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302541.6126434/94‑1 (MQ=60)
                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302541.8313166/94‑1 (MQ=60)
                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302541.8587868/94‑1 (MQ=60)
                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302541.1594218/94‑1 (MQ=60)
                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    >  SRR4302541.4103427/1‑94 (MQ=60)
                                                                                              CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   >  SRR4302541.3451709/1‑94 (MQ=60)
                                                                                              CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   >  SRR4302541.1348376/1‑94 (MQ=60)
                                                                                              CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   >  SRR4302541.4430174/1‑94 (MQ=60)
                                                                                              CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   <  SRR4302541.2246095/94‑1 (MQ=60)
                                                                                               CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  <  SRR4302541.953089/94‑1 (MQ=60)
                                                                                               CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302541.1103822/1‑94 (MQ=60)
                                                                                               CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  <  SRR4302541.2721762/94‑1 (MQ=60)
                                                                                               CTCATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302541.330926/1‑94 (MQ=60)
                                                                                               |                                                                                             
CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013680‑4013865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑