Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,019,860 Δ2 bp intergenic (+59/‑24) b3836 → / → b3838 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,019,8600C.99.8% 2251.0 / ‑3.8 485intergenic (+59/‑25)b3836/b3838hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (272/212);  total (273/212)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01
*NC_0009134,019,8610C.99.8% 2255.0 / ‑4.0 485intergenic (+60/‑24)b3836/b3838hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (272/212);  minor base G (1/0);  total (273/212)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 500 total aligned reads displayed.

AAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGG  >  NC_000913/4019775‑4019942
                                                                                     ||                                                                                 
aaGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTgggg                                                                           >  1:7416322/1‑93 (MQ=255)
 aGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc                                                                          >  2:806365/1‑93 (MQ=255)
  gcgcCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc                                                                         >  1:7094295/1‑93 (MQ=255)
    gcCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGc                                                                       >  1:265754/1‑93 (MQ=255)
    gcCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGc                                                                       <  1:4233438/93‑1 (MQ=255)
    gacACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGc                                                                       <  1:2059374/91‑1 (MQ=255)
     ccACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCa                                                                      >  2:8806692/1‑93 (MQ=255)
     ccACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCa                                                                      >  1:6660881/1‑93 (MQ=255)
      cACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCaa                                                                     <  1:6895898/93‑1 (MQ=255)
       aCGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAc                                                                    >  2:8399604/1‑93 (MQ=255)
       aCGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAc                                                                    >  1:8513305/1‑93 (MQ=255)
         gATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGa                                                                  <  2:4980963/93‑1 (MQ=255)
         gATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGa                                                                  >  1:8648153/1‑93 (MQ=255)
         gATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGa                                                                  >  1:5262630/1‑93 (MQ=255)
         gATAAAGAGCAGGTGTAATCCGTGTTTGATATCAGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGa                                                                  <  2:1260493/93‑1 (MQ=255)
          aTAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAc                                                                 <  1:4979152/93‑1 (MQ=255)
           tAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACt                                                                <  1:3143017/93‑1 (MQ=255)
           tAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACt                                                                >  2:4804774/1‑93 (MQ=255)
            aaaGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACtc                                                               >  1:6440641/1‑92 (MQ=255)
             aaGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGc                                                              >  2:1725826/1‑93 (MQ=255)
             aaGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGc                                                              >  1:4504147/1‑93 (MQ=255)
              agagCAGGTGTAATCCGTGTTTGATATCGGTTGTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGcc                                                             <  1:5495250/93‑1 (MQ=255)
                 gCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgt                                                          >  1:2826032/1‑93 (MQ=255)
                 gCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgt                                                          >  2:8186837/1‑93 (MQ=255)
                 gCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgt                                                          <  2:1639141/93‑1 (MQ=255)
                   aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg                                                        <  2:7556009/93‑1 (MQ=255)
                   aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg                                                        >  1:4174304/1‑93 (MQ=255)
                   aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg                                                        >  2:5219746/1‑93 (MQ=255)
                   aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg                                                        >  2:1472714/1‑93 (MQ=255)
                     gtgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTGTTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCg                                                      <  2:2366980/93‑1 (MQ=255)
                     gtgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCg                                                      >  1:3024929/1‑93 (MQ=255)
                     gtgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCg                                                      >  1:3592202/1‑93 (MQ=255)
                      tgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCATGTGGCgg                                                     >  1:7618798/1‑93 (MQ=255)
                       gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGt                                                    <  1:5804051/93‑1 (MQ=255)
                       gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGt                                                    >  1:387780/1‑93 (MQ=255)
                        tAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTa                                                   >  2:3619067/1‑93 (MQ=255)
                         aaTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaa                                                  >  2:3168757/1‑93 (MQ=255)
                         aaTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaa                                                  >  1:7889511/1‑93 (MQ=255)
                          aTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaa                                                 >  1:895070/1‑93 (MQ=255)
                          aTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCAGCCT‑‑CTGCGTTCTGGGGCCGCAACGACTGCCTGTGGCGGCaaa                                                 >  2:5495352/1‑93 (MQ=255)
                           tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaaa                                                <  2:6013807/93‑1 (MQ=255)
                           tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaaa                                                >  2:5367156/1‑93 (MQ=255)
                           tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaaa                                                >  1:4399954/1‑93 (MQ=255)
                            ctgtgtgtGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaaaa                                               <  2:8654535/91‑1 (MQ=255)
                             cGTGTTTGATGTCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAAc                                              <  1:226464/93‑1 (MQ=255)
                             cGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAAc                                              <  1:6999996/93‑1 (MQ=255)
                             cGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAAc                                              <  2:4614254/93‑1 (MQ=255)
                              gtgtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACg                                             <  2:4359505/93‑1 (MQ=255)
                              gtgtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGCCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACg                                             <  1:1856588/93‑1 (MQ=255)
                               tgtTTGATATCGGTTTTAGCGAACCGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGCCTGCCTGTGGCGGTAAAAACgg                                            >  1:7523087/1‑93 (MQ=255)
                                gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGt                                           <  2:6406448/93‑1 (MQ=255)
                                 tttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                          <  2:136172/93‑1 (MQ=255)
                                 tttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                          >  1:3283554/1‑93 (MQ=255)
                                 gttGATATCGGTTTTAGTTAACTGCTATTGCTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCGGTGTCGGTAAAAACGGTa                                          <  1:4844833/92‑1 (MQ=255)
                                  ttGATATCGGTTTTAGCTAGCTGTTGTTGGTGTTCATCATCGCGCTCGTAG‑‑TGCTGGGGCCGCAACGACTGCCTGTGTCGGTAAAAACGGTAg                                         <  2:4732303/93‑1 (MQ=255)
                                  ttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAg                                         >  1:6148258/1‑93 (MQ=255)
                                   tGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAgg                                        >  2:2247618/1‑92 (MQ=255)
                                   tGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGc                                        <  1:6057633/93‑1 (MQ=255)
                                     atatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCgg                                      >  2:4038436/1‑93 (MQ=255)
                                      tatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCggg                                     >  2:3721447/1‑93 (MQ=255)
                                       atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGAAAAACGGTAGCGGGc                                    >  1:1186948/1‑93 (MQ=255)
                                         cGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTg                                  <  2:2184319/93‑1 (MQ=255)
                                         cGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTg                                  <  1:5675405/93‑1 (MQ=255)
                                           gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTggt                                >  1:6829241/1‑92 (MQ=255)
                                           gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGa                                >  2:2508853/1‑93 (MQ=255)
                                           gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGa                                <  2:2856212/93‑1 (MQ=255)
                                           gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGa                                >  1:730388/1‑93 (MQ=255)
                                           gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGAAAAAAGGTAGCGGGCTGGa                                >  2:5885382/1‑93 (MQ=255)
                                             tttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAtt                              >  1:1017259/1‑93 (MQ=255)
                                             tttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAtt                              >  2:2983524/1‑93 (MQ=255)
                                                aGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGGTTcgc                           >  2:6724821/1‑93 (MQ=255)
                                                aGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgc                           >  1:6348370/1‑93 (MQ=255)
                                                 gCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgcg                          >  1:240521/1‑93 (MQ=255)
                                                   gAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgcgcg                        >  2:6604417/1‑93 (MQ=255)
                                                   gAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgcgcg                        <  1:2700355/93‑1 (MQ=255)
                                                    aaCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgt                       >  1:3774073/1‑93 (MQ=255)
                                                     aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgtt                      <  1:8001672/93‑1 (MQ=255)
                                                     aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgtt                      >  1:8356279/1‑93 (MQ=255)
                                                     aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgtt                      <  1:7834186/93‑1 (MQ=255)
                                                      cTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGAAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttg                     <  2:4131793/93‑1 (MQ=255)
                                                         cTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttgcgt                  >  1:4755887/1‑93 (MQ=255)
                                                           aTTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTc                >  1:6527696/1‑93 (MQ=255)
                                                           aTTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTc                >  1:3398780/1‑93 (MQ=255)
                                                           aTTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTc                <  2:7346455/93‑1 (MQ=255)
                                                           aTTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTc                <  2:3838354/93‑1 (MQ=255)
                                                             tGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCAc              <  1:1296177/93‑1 (MQ=255)
                                                             tGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCAc              <  1:1659730/93‑1 (MQ=255)
                                                              ggTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACt             >  2:3366157/1‑93 (MQ=255)
                                                               gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTg            >  1:818873/1‑93 (MQ=255)
                                                                tgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGAAAAACGGAGGCGGGCTGGATTGGCGCGTTGCGTTCACCgg           >  2:6849508/1‑93 (MQ=255)
                                                                  ttCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCg         >  1:1559115/1‑93 (MQ=255)
                                                                  ttCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCg         >  2:2738608/1‑93 (MQ=255)
                                                                   tcatcaTCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGa        <  2:6123664/93‑1 (MQ=255)
                                                                    catcatCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGAc       >  2:1991762/1‑93 (MQ=255)
                                                                    catcatCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGAc       >  2:1172932/1‑93 (MQ=255)
                                                                      tcatcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGACTCGCGCGTTGCGTTCACTGGCGACaa     >  2:5616432/1‑93 (MQ=255)
                                                                       catcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAAc    <  2:7931271/93‑1 (MQ=255)
                                                                       catcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAAc    <  1:1996794/93‑1 (MQ=255)
                                                                        atcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACg   >  2:7160596/1‑93 (MQ=255)
                                                                         tcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACgg  <  1:5363406/93‑1 (MQ=255)
                                                                                     ||                                                                                 
AAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGG  >  NC_000913/4019775‑4019942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 301 total aligned reads displayed.
GCGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGTGCAGAACGAA  >  NC_000913/4019772‑4019953
                                                                                        |                                                                                             
GCGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGG                                                                                        >  SRR4302541.7855130/1‑94 (MQ=60)
GCGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGG                                                                                        >  SRR4302541.6848618/1‑94 (MQ=60)
GCGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGG                                                                                        <  SRR4302541.2791153/94‑1 (MQ=60)
GCGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGG                                                                                        <  SRR4302541.3101057/94‑1 (MQ=60)
GCGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTGTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGG                                                                                        <  SRR4302541.7178887/94‑1 (MQ=60)
 CGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGG                                                                                       >  SRR4302541.7667426/1‑94 (MQ=60)
 CGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGG                                                                                       >  SRR4302541.2265551/1‑94 (MQ=60)
 CGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGG                                                                                       <  SRR4302541.772237/94‑1 (MQ=60)
 CGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGG                                                                                       >  SRR4302541.3825166/1‑94 (MQ=60)
  GAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGG                                                                                      >  SRR4302541.7495627/1‑94 (MQ=60)
  GAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGG                                                                                      >  SRR4302541.2684573/1‑94 (MQ=60)
  GAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGG                                                                                      >  SRR4302541.697814/1‑94 (MQ=60)
   AAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGC                                                                                     <  SRR4302541.259328/94‑1 (MQ=60)
    AGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCC                                                                                    >  SRR4302541.7169779/1‑94 (MQ=60)
    AGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCC                                                                                    <  SRR4302541.1002962/94‑1 (MQ=60)
    AGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCC                                                                                    <  SRR4302541.8121796/94‑1 (MQ=60)
     GCGTCACGATAAAGAGCAGGTGTAATCCGTGTTTTATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCG                                                                                   <  SRR4302541.3381569/94‑1 (MQ=60)
      CGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGC                                                                                  >  SRR4302541.267934/1‑94 (MQ=60)
      CGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGC                                                                                  >  SRR4302541.3815304/1‑94 (MQ=60)
      CGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGC                                                                                  >  SRR4302541.8698611/1‑94 (MQ=60)
      CGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGC                                                                                  <  SRR4302541.7051015/94‑1 (MQ=60)
      CGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGC                                                                                  >  SRR4302541.3233463/1‑94 (MQ=60)
       GCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATGTCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCA                                                                                 >  SRR4302541.3347363/1‑94 (MQ=60)
       GCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCA                                                                                 >  SRR4302541.6729224/1‑94 (MQ=60)
       GCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCA                                                                                 <  SRR4302541.4275630/94‑1 (MQ=60)
        CCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAA                                                                                >  SRR4302541.4040191/1‑94 (MQ=60)
         CACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAC                                                                               <  SRR4302541.7433219/94‑1 (MQ=60)
         CACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAC                                                                               >  SRR4302541.1229353/1‑94 (MQ=60)
         CACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAC                                                                               <  SRR4302541.6969483/94‑1 (MQ=60)
         CACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAC                                                                               >  SRR4302541.8484485/1‑94 (MQ=60)
         CACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAC                                                                               >  SRR4302541.2804085/1‑94 (MQ=60)
         CACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAAC                                                                               >  SRR4302541.2687684/1‑94 (MQ=60)
          ACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACG                                                                              >  SRR4302541.7148598/1‑94 (MQ=60)
          ACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACG                                                                              <  SRR4302541.500485/94‑1 (MQ=60)
          ACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACG                                                                              >  SRR4302541.5915155/1‑94 (MQ=60)
           CGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGA                                                                             >  SRR4302541.8740760/1‑94 (MQ=60)
           CGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGA                                                                             >  SRR4302541.2898179/1‑94 (MQ=60)
            GATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAC                                                                            >  SRR4302541.2129382/1‑94 (MQ=60)
            GATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAC                                                                            >  SRR4302541.4493654/1‑94 (MQ=60)
            GATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAC                                                                            <  SRR4302541.4501261/94‑1 (MQ=60)
            GATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAC                                                                            >  SRR4302541.1282246/1‑94 (MQ=60)
             ATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACT                                                                           <  SRR4302541.4319576/94‑1 (MQ=60)
             ATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACT                                                                           <  SRR4302541.7524517/94‑1 (MQ=60)
              TAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTG                                                                          <  SRR4302541.3597011/94‑1 (MQ=60)
              TAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTG                                                                          <  SRR4302541.3174760/94‑1 (MQ=60)
               AAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGC                                                                         <  SRR4302541.8235768/94‑1 (MQ=60)
               AAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGC                                                                         >  SRR4302541.4549580/1‑94 (MQ=60)
               AAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGC                                                                         <  SRR4302541.1869356/94‑1 (MQ=60)
                AAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAATGACTGCC                                                                        >  SRR4302541.7540878/1‑94 (MQ=60)
                 AGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCT                                                                       <  SRR4302541.2379506/94‑1 (MQ=60)
                 AGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCT                                                                       <  SRR4302541.2141634/94‑1 (MQ=60)
                 AGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCT                                                                       >  SRR4302541.654846/1‑94 (MQ=60)
                 AGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑GTCTGGGGCCGCAACGACTGCCT                                                                       >  SRR4302541.7880634/1‑94 (MQ=60)
                 AGAGCAGGTGTAATCCGTGTTTGATATCGGTTGTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCT                                                                       <  SRR4302541.5551558/94‑1 (MQ=60)
                   AGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGT                                                                     <  SRR4302541.1653922/94‑1 (MQ=60)
                   AGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGT                                                                     >  SRR4302541.2854528/1‑94 (MQ=60)
                   AGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGT                                                                     <  SRR4302541.6193999/94‑1 (MQ=60)
                    GCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTG                                                                    >  SRR4302541.2079097/1‑94 (MQ=60)
                    GCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTG                                                                    >  SRR4302541.8002095/1‑94 (MQ=60)
                     CAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAAGTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TCCTGGGGCCGCAACGACTGCCTGTAG                                                                   >  SRR4302541.6669441/1‑94 (MQ=60)
                      AGGTGTAATCCGTGTTTTAGATCGGTTTTAGCGAACTGCGATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC                                                                  <  SRR4302541.7786169/94‑1 (MQ=60)
                      AGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC                                                                  >  SRR4302541.1489952/1‑94 (MQ=60)
                      AGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC                                                                  >  SRR4302541.6902225/1‑94 (MQ=60)
                      AGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC                                                                  >  SRR4302541.1489954/1‑94 (MQ=60)
                      AGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGC                                                                  >  SRR4302541.2235438/1‑94 (MQ=60)
                       GGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCG                                                                 >  SRR4302541.3628622/1‑94 (MQ=60)
                       GGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCG                                                                 >  SRR4302541.6336510/1‑94 (MQ=60)
                        GTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGG                                                                >  SRR4302541.3447672/1‑94 (MQ=60)
                         TGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGT                                                               >  SRR4302541.5176085/1‑94 (MQ=60)
                         TGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGT                                                               >  SRR4302541.391365/1‑94 (MQ=60)
                         TGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGT                                                               >  SRR4302541.3212315/1‑94 (MQ=60)
                          GTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTA                                                              >  SRR4302541.5379106/1‑94 (MQ=60)
                          GTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTA                                                              <  SRR4302541.5862990/94‑1 (MQ=60)
                           TAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAA                                                             >  SRR4302541.86693/1‑94 (MQ=60)
                            AATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAA                                                            >  SRR4302541.903313/1‑94 (MQ=60)
                            AATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAA                                                            >  SRR4302541.5330455/1‑94 (MQ=60)
                             ATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAA                                                           >  SRR4302541.490879/1‑94 (MQ=60)
                             ATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAA                                                           >  SRR4302541.4443964/1‑94 (MQ=60)
                               CCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAAC                                                         <  SRR4302541.528828/94‑1 (MQ=60)
                               CCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAAC                                                         <  SRR4302541.3393438/94‑1 (MQ=60)
                                CGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACG                                                        >  SRR4302541.5683024/1‑94 (MQ=60)
                                CGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACG                                                        <  SRR4302541.6469299/94‑1 (MQ=60)
                                CGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTCGGGCCGCAACGACTGCCTGTGGCGGTAAAAACG                                                        >  SRR4302541.793869/1‑94 (MQ=60)
                                 GTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCGC‑‑TCTGGGGCCGCGACGACTGACTGTGGCGGTACAAACGG                                                       >  SRR4302541.6573286/1‑94 (MQ=60)
                                 GTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCCGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGG                                                       >  SRR4302541.4260711/1‑94 (MQ=60)
                                 GTGTTTGATATCGGTTTTAGCGAACTGCTATTGGGGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGG                                                       >  SRR4302541.7201184/1‑94 (MQ=60)
                                 GTGTTTGATATCGGTTTTAGCGAACCGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGCCTGCCTGTGGCGGTAAAAACGG                                                       >  SRR4302541.7603478/1‑94 (MQ=60)
                                  TGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGT                                                      <  SRR4302541.7653790/94‑1 (MQ=60)
                                   GTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTG                                                     >  SRR4302541.8355200/1‑94 (MQ=60)
                                   GTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTA                                                     <  SRR4302541.8326208/94‑1 (MQ=60)
                                   GTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTA                                                     >  SRR4302541.3316642/1‑94 (MQ=60)
                                    TTTTATATCGGTTTTAGCGAACTGCTATTGGTTTTCATCATCGGTCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAG                                                    <  SRR4302541.6367669/94‑1 (MQ=60)
                                    TTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAG                                                    <  SRR4302541.8735313/94‑1 (MQ=60)
                                    TTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAG                                                    <  SRR4302541.1548135/94‑1 (MQ=60)
                                    TTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAG                                                    >  SRR4302541.6211076/1‑94 (MQ=60)
                                    TTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAG                                                    <  SRR4302541.285617/94‑1 (MQ=60)
                                    TTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAG                                                    <  SRR4302541.7955122/94‑1 (MQ=60)
                                    TTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAG                                                    <  SRR4302541.4894213/94‑1 (MQ=60)
                                     TTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGC                                                   >  SRR4302541.7996739/1‑94 (MQ=60)
                                     TTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGC                                                   <  SRR4302541.7121448/94‑1 (MQ=60)
                                     TTGATATCGGTTTTAGCGAACCGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGC                                                   <  SRR4302541.5481008/94‑1 (MQ=60)
                                      TGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCG                                                  <  SRR4302541.6119532/94‑1 (MQ=60)
                                      TGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCG                                                  >  SRR4302541.2726808/1‑94 (MQ=60)
                                      TGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCG                                                  >  SRR4302541.2558224/1‑94 (MQ=60)
                                        ATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGG                                                <  SRR4302541.130540/94‑1 (MQ=60)
                                        ATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGG                                                <  SRR4302541.4206998/94‑1 (MQ=60)
                                        ATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGG                                                <  SRR4302541.5476723/94‑1 (MQ=60)
                                         TATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGAAAAACGGTAGCGGGC                                               >  SRR4302541.1198432/1‑94 (MQ=60)
                                          ATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCT                                              >  SRR4302541.6928571/1‑94 (MQ=60)
                                          ATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCT                                              >  SRR4302541.6955113/1‑94 (MQ=60)
                                          ATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCT                                              <  SRR4302541.6226872/94‑1 (MQ=60)
                                          ATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCT                                              <  SRR4302541.2078324/94‑1 (MQ=60)
                                          ATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCT                                              <  SRR4302541.3707189/94‑1 (MQ=60)
                                          ATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGAAAAACGGTAGCGGGCT                                              >  SRR4302541.7920772/1‑94 (MQ=60)
                                           TCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTG                                             <  SRR4302541.1798377/94‑1 (MQ=60)
                                            CGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGG                                            <  SRR4302541.5733321/94‑1 (MQ=60)
                                            CGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGG                                            <  SRR4302541.2360782/94‑1 (MQ=60)
                                             GGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGA                                           <  SRR4302541.3520108/94‑1 (MQ=60)
                                              GTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAT                                          >  SRR4302541.7063041/1‑94 (MQ=60)
                                              GTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAT                                          <  SRR4302541.1956977/94‑1 (MQ=60)
                                              GTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAT                                          >  SRR4302541.4651991/1‑94 (MQ=60)
                                               TTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTTG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATT                                         <  SRR4302541.4881412/94‑1 (MQ=60)
                                               TTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATT                                         <  SRR4302541.740231/94‑1 (MQ=60)
                                               TTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATT                                         <  SRR4302541.740232/94‑1 (MQ=60)
                                               TTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATT                                         >  SRR4302541.1026661/1‑94 (MQ=60)
                                                TTTAGCGAACTGCTATTGGTGTTCATCATCGGCTGCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTC                                        <  SRR4302541.8600811/94‑1 (MQ=60)
                                                TTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTC                                        <  SRR4302541.2476061/94‑1 (MQ=60)
                                                 TTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCG                                       <  SRR4302541.5515285/94‑1 (MQ=60)
                                                  TAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGC                                      >  SRR4302541.6413263/1‑94 (MQ=60)
                                                   AGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCG                                     >  SRR4302541.242437/1‑94 (MQ=60)
                                                    GCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGC                                    <  SRR4302541.1901259/94‑1 (MQ=60)
                                                    GCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGAAGCGGGCTGGATTCGCGC                                    >  SRR4302541.2752634/1‑94 (MQ=60)
                                                     GGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCG                                   <  SRR4302541.8796653/94‑1 (MQ=60)
                                                      GAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGT                                  >  SRR4302541.3811983/1‑94 (MQ=60)
                                                      GAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGT                                  >  SRR4302541.3934766/1‑94 (MQ=60)
                                                      GAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGT                                  >  SRR4302541.6475996/1‑94 (MQ=60)
                                                       AACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTT                                 >  SRR4302541.7327276/1‑94 (MQ=60)
                                                       AACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTT                                 <  SRR4302541.5102689/94‑1 (MQ=60)
                                                       AACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTT                                 >  SRR4302541.8446940/1‑94 (MQ=60)
                                                        ACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTG                                >  SRR4302541.6683370/1‑94 (MQ=60)
                                                        ACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTG                                <  SRR4302541.7919568/94‑1 (MQ=60)
                                                        ACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTG                                <  SRR4302541.8514670/94‑1 (MQ=60)
                                                        ACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTG                                <  SRR4302541.8089711/94‑1 (MQ=60)
                                                         CTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGC                               >  SRR4302541.8121405/1‑94 (MQ=60)
                                                         CTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGC                               <  SRR4302541.5636119/94‑1 (MQ=60)
                                                         CTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGC                               <  SRR4302541.4852165/94‑1 (MQ=60)
                                                          TGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCG                              >  SRR4302541.3864014/1‑94 (MQ=60)
                                                          TGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCG                              <  SRR4302541.2332662/94‑1 (MQ=60)
                                                          TGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGCCAAAACGGTAGCGGGCTGGATTCGCGCGTTGCG                              >  SRR4302541.6175559/1‑94 (MQ=60)
                                                           GCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGT                             >  SRR4302541.4804472/1‑94 (MQ=60)
                                                           GCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGT                             <  SRR4302541.8054284/94‑1 (MQ=60)
                                                            CTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTT                            <  SRR4302541.4534051/94‑1 (MQ=60)
                                                            CTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTT                            >  SRR4302541.4268897/1‑94 (MQ=60)
                                                            CTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATGCGCGCGTTGCGTT                            >  SRR4302541.4629375/1‑94 (MQ=60)
                                                             TATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTC                           >  SRR4302541.1974339/1‑94 (MQ=60)
                                                             TATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTC                           >  SRR4302541.6594324/1‑94 (MQ=60)
                                                              ATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCA                          >  SRR4302541.422701/1‑94 (MQ=60)
                                                              ATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCA                          <  SRR4302541.5337308/94‑1 (MQ=60)
                                                               TTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCAC                         <  SRR4302541.1160883/94‑1 (MQ=60)
                                                                TGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACT                        <  SRR4302541.5600057/94‑1 (MQ=60)
                                                                TGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACT                        <  SRR4302541.1676103/94‑1 (MQ=60)
                                                                TGGTGATCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACT                        <  SRR4302541.5754510/94‑1 (MQ=60)
                                                                 GGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTG                       <  SRR4302541.1632395/94‑1 (MQ=60)
                                                                 GGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTG                       <  SRR4302541.3706424/94‑1 (MQ=60)
                                                                 GGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTG                       <  SRR4302541.1138293/94‑1 (MQ=60)
                                                                 GGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTG                       >  SRR4302541.826337/1‑94 (MQ=60)
                                                                  GTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGG                      <  SRR4302541.1896225/94‑1 (MQ=60)
                                                                   TGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGC                     <  SRR4302541.2773972/94‑1 (MQ=60)
                                                                   TGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGGAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGCTCACTGGG                     >  SRR4302541.7284436/1‑94 (MQ=60)
                                                                    GTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCG                    >  SRR4302541.7100758/1‑94 (MQ=60)
                                                                    GTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCG                    <  SRR4302541.186027/94‑1 (MQ=60)
                                                                    GTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCG                    >  SRR4302541.1574730/1‑94 (MQ=60)
                                                                       CATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTTTGGCGGTAAAAACGGTAGCGGTCTGGACTCGCGCGTTGCGTTCACTGGCGACA                 >  SRR4302541.5867395/1‑94 (MQ=60)
                                                                       CATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACA                 <  SRR4302541.2326841/94‑1 (MQ=60)
                                                                       CATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACA                 >  SRR4302541.4349379/1‑94 (MQ=60)
                                                                       CATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATGCGCGCGTTGCGTTCACTGGCGCCA                 >  SRR4302541.6928507/1‑94 (MQ=60)
                                                                        ATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAA                >  SRR4302541.6829834/1‑94 (MQ=60)
                                                                        ATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAA                >  SRR4302541.764281/1‑94 (MQ=60)
                                                                        ATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAA                >  SRR4302541.2891227/1‑94 (MQ=60)
                                                                        ATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAA                >  SRR4302541.5882991/1‑94 (MQ=60)
                                                                        ATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAA                <  SRR4302541.2020257/94‑1 (MQ=60)
                                                                         TCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAAC               >  SRR4302541.6506749/1‑94 (MQ=60)
                                                                          CATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACG              >  SRR4302541.888269/1‑94 (MQ=60)
                                                                          CATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACG              <  SRR4302541.2016120/94‑1 (MQ=60)
                                                                          CATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACG              <  SRR4302541.3158669/94‑1 (MQ=60)
                                                                           ATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGG             <  SRR4302541.4922567/94‑1 (MQ=60)
                                                                           ATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGG             <  SRR4302541.6417248/94‑1 (MQ=60)
                                                                           ATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGG             <  SRR4302541.3102655/94‑1 (MQ=60)
                                                                            TCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGT            <  SRR4302541.7663117/94‑1 (MQ=60)
                                                                            TCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGT            <  SRR4302541.5418487/94‑1 (MQ=60)
                                                                            TCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGT            <  SRR4302541.5755791/94‑1 (MQ=60)
                                                                            TCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGT            >  SRR4302541.6802601/1‑94 (MQ=60)
                                                                            TCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGG            >  SRR4302541.6954856/1‑94 (MQ=60)
                                                                            TCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGG            >  SRR4302541.3142508/1‑94 (MQ=60)
                                                                             CGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGTG           <  SRR4302541.6866752/94‑1 (MQ=60)
                                                                              GGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGTGC          >  SRR4302541.5849107/1‑94 (MQ=60)
                                                                               GCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGTGCA         >  SRR4302541.436391/1‑94 (MQ=60)
                                                                                        CGTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCAGTGGCGACAACGGTGCAGAACGAA  >  SRR4302541.4951127/1‑94 (MQ=60)
                                                                                        CGTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGTGCAGAACGAA  <  SRR4302541.7525894/94‑1 (MQ=60)
                                                                                        CGTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCAGGCTGGATTCGCGCGTTGCGTTCACTGGCGCCAACGGTGCAGAACGAA  >  SRR4302541.3112471/1‑94 (MQ=60)
                                                                                        |                                                                                             
GCGAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGTGCAGAACGAA  >  NC_000913/4019772‑4019953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑