Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,543	+C	intergenic (+48/‑90)	ytfQ → / → ytfR	putative LACI‑type transcriptional regulator/YtfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,542	1	.	C	98.9%	823.0 / 1.2	283	intergenic (+47/‑91)	ytfQ/ytfR	putative LACI‑type transcriptional regulator/YtfR
Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (182/98); total (184/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 304 total aligned reads displayed.

AAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CG‑‑G‑‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGG  >  NC_000913/4448461‑4448624

aagaaTTAGAGAAGAAGAAAAACATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCgg                                                                           >  2:5525875/1‑93 (MQ=37)

 agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGc                                                                          >  2:3569382/1‑93 (MQ=37)

 agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGc                                                                          >  1:3940437/1‑93 (MQ=37)

  gaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCg                                                                         >  2:318693/1‑93 (MQ=37)

   agttAGAGAAAAAGAAAAATAGGGGGTATTGAGGGTTGCTATGCTTGATGCAGCTTCGTAGGCAGGATAAGGCCCT‑‑CGC‑GC‑C‑GCATCCGGCGa                                                                        <  2:6569639/91‑1 (MQ=19)

   aaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGa                                                                        <  2:8510249/93‑1 (MQ=37)

   aaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGa                                                                        >  2:2781427/1‑93 (MQ=37)

    aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGAt                                                                       >  2:4895387/1‑93 (MQ=37)

     ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATg                                                                      >  1:3486067/1‑93 (MQ=37)

      tAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATgg                                                                     >  2:262036/1‑93 (MQ=37)

       agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGt                                                                    >  1:7401540/1‑93 (MQ=37)

       agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGt                                                                    >  1:1676856/1‑93 (MQ=37)

        gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTg                                                                   >  2:6755069/1‑93 (MQ=37)

          gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCa                                                                 >  2:4644095/1‑93 (MQ=37)

           aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCAc                                                                <  2:5910265/93‑1 (MQ=37)

           aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCAc                                                                >  2:6336071/1‑93 (MQ=37)

             gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTg                                                              >  2:1075839/1‑93 (MQ=37)

             gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTg                                                              >  1:5255103/1‑93 (MQ=37)

             gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTg                                                              >  1:2146213/1‑93 (MQ=37)

               agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGaa                                                            >  1:5721958/1‑93 (MQ=37)

               agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGaa                                                            <  1:548672/93‑1 (MQ=37)

                gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAg                                                           >  2:2426577/1‑93 (MQ=37)

                aaaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGGTAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAg                                                           <  1:6420096/92‑1 (MQ=37)

                 aaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGc                                                          <  2:4076569/93‑1 (MQ=37)

                 aaaaaT‑TGGGATATTGGGGTTTGCTTTGCCTGGTGCCATTTCGTAGGCAGGATAGGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGcc                                                         <  2:6670500/93‑1 (MQ=12)

                  aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGcc                                                         <  1:8165233/93‑1 (MQ=37)

                    aaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTg                                                       <  1:4754095/93‑1 (MQ=37)

                    aaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTg                                                       >  1:7023486/1‑93 (MQ=37)

                     atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGa                                                      >  1:6215627/1‑93 (MQ=37)

                     atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGa                                                      <  1:886804/93‑1 (MQ=37)

                      tatGTGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGAt                                                     >  1:6012642/1‑93 (MQ=25)

                      tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGCAC‑‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGAt                                                     >  2:1488424/1‑93 (MQ=37)

                        tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                   >  1:7536041/1‑93 (MQ=25)

                        tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                   >  2:1697984/1‑93 (MQ=25)

                        tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                   <  2:4133560/93‑1 (MQ=25)

                          gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGa                                                 >  1:4977551/1‑93 (MQ=25)

                          gggTATGGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGa                                                 <  1:3346380/93‑1 (MQ=25)

                           ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGag                                                >  2:3034822/1‑92 (MQ=25)

                           ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGAc                                                >  1:3062627/1‑93 (MQ=25)

                             tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACgt                                              >  1:2504513/1‑92 (MQ=25)

                              aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCt                                             >  2:8710589/1‑93 (MQ=25)

                               ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCtt                                            >  2:8604866/1‑93 (MQ=25)

                               ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCtt                                            <  1:5585427/93‑1 (MQ=25)

                                tGAGGTTTGCTAGGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑G‑‑CGGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa                                           <  2:3935510/93‑1 (MQ=25)

                                 gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                          >  2:7737596/1‑93 (MQ=25)

                                  aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                         >  1:4161228/1‑93 (MQ=25)

                                  aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                         >  2:6939910/1‑93 (MQ=25)

                                  aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                         <  1:3975537/93‑1 (MQ=25)

                                   gggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg                                        >  2:3647605/1‑93 (MQ=25)

                                    ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                       <  1:2707275/93‑1 (MQ=25)

                                      ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                     <  1:1449261/93‑1 (MQ=25)

                                         cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCtt                                  >  1:6544935/1‑93 (MQ=25)

                                          tATGCCTGATGCCGATTCGTAGGCCGTATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                 <  1:4078246/93‑1 (MQ=21)

                                          tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGGGATGGTGCACTGAAGCCTGAGGGGACGCTTCCCGCGTCttt                                 >  2:4164535/1‑92 (MQ=21)

                                          tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                 >  2:7297823/1‑93 (MQ=25)

                                           ttGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAt                                <  1:5091800/92‑1 (MQ=25)

                                            tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGAGGCTTACCGCGTCTTAta                               >  1:7196502/1‑92 (MQ=21)

                                            tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                               >  2:3838376/1‑93 (MQ=21)

                                             gCCTGATGCCGATTCGTAGGCCTGATAAGGCGCT‑‑CG‑‑GGCC‑GCATCCGGCGATGGGGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                              <  2:2106233/93‑1 (MQ=25)

                                             gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                              >  1:77976/1‑93 (MQ=21)

                                             gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                              >  2:7416376/1‑93 (MQ=21)

                                              ccTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt                             <  1:4611200/93‑1 (MQ=21)

                                              ccTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt                             >  2:2498373/1‑93 (MQ=21)

                                               cTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg                            <  2:5264909/93‑1 (MQ=21)

                                               cTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg                            >  1:1164548/1‑93 (MQ=21)

                                               cTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg                            >  1:3427103/1‑93 (MQ=21)

                                                tGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCTACGCTTACCGCGTCTTATCATGc                           >  2:4762589/1‑93 (MQ=21)

                                                 gATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGcc                          >  2:6184649/1‑93 (MQ=21)

                                                 gATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGcc                          <  1:835248/93‑1 (MQ=21)

                                                  aTGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCt                         >  1:7945910/1‑93 (MQ=21)

                                                    gCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGATGCACTGAAGCCTGATGAGACGCTTACCGCGTCTTCTCATGCCTAc                       >  2:4313346/1‑93 (MQ=18)

                                                    gCCGATGCGTAGGCCGGATAAGGCGCG‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAc                       <  1:2046844/93‑1 (MQ=18)

                                                     ccGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACt                      <  1:4238720/93‑1 (MQ=21)

                                                     ccGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACt                      >  2:2127818/1‑93 (MQ=21)

                                                      cGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg                     >  1:8688923/1‑93 (MQ=21)

                                                       gATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                    >  1:7718637/1‑93 (MQ=21)

                                                       gATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                    >  2:5196324/1‑93 (MQ=21)

                                                       gATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                    >  2:6417215/1‑93 (MQ=18)

                                                        aTTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                   <  1:8524689/93‑1 (MQ=21)

                                                        aTTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                   <  2:2191645/93‑1 (MQ=18)

                                                         ttggTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                  <  1:1915117/90‑1 (MQ=18)

                                                         ttCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                  >  1:2810437/1‑93 (MQ=21)

                                                         ttCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                  <  2:6515198/93‑1 (MQ=21)

                                                          tCGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAg                 <  1:444971/93‑1 (MQ=18)

                                                           cGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGc                >  2:3320827/1‑93 (MQ=21)

                                                           cGTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGc                >  2:7116679/1‑93 (MQ=21)

                                                            gTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCa               >  1:3667557/1‑93 (MQ=21)

                                                            gTAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCa               >  1:1310128/1‑93 (MQ=21)

                                                             tAGTCCGTATAAGGCGCTCGCG‑‑G‑‑C‑GCATCCGGCGATGGTGCAGTGAACCCTGATGCGACGCTTACCTCGTCTTATCATGCCTACTGGGAGCAc              <  1:4304333/93‑1 (MQ=21)

                                                             tAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAc              >  1:8564414/1‑93 (MQ=21)

                                                             tAGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAc              <  1:1033167/93‑1 (MQ=21)

                                                              aGGCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACg             <  1:2925072/93‑1 (MQ=21)

                                                                gacGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCt           <  1:6836651/91‑1 (MQ=21)

                                                                gCCGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCt           >  2:1031794/1‑93 (MQ=21)

                                                                  cGGATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCttt         <  2:8274035/93‑1 (MQ=21)

                                                                    gATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTac       >  2:598337/1‑93 (MQ=21)

                                                                    gATAAGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTac       >  1:7321512/1‑93 (MQ=21)

                                                                      aaaGGCGCT‑‑CGC‑GC‑C‑GCATCGGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTacac     <  2:8002522/92‑1 (MQ=25)

                                                                       ggcgcgcg‑‑cg‑‑c‑‑C‑GCAGCCGGCGATGGGGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCgg  <  2:2231306/90‑1 (MQ=37)

                                                                        aGGCGCT‑‑CGC‑GC‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg   >  2:1303527/1‑93 (MQ=25)

AAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CG‑‑G‑‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGG  >  NC_000913/4448461‑4448624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A