Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,068 +C intergenic (‑178/‑242) b4256 ← / → yjgN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,0681.C99.7% 1086.2 / ‑6.4 315intergenic (‑178/‑242)b4256/yjgNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (99/214);  minor base A (1/0);  total (100/215)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 335 total aligned reads displayed.

GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476975‑4477153
                                                                                              |                                                                                     
 gtgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgc                                                                                       <  2:520571/93‑2 (MQ=255)
  tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgca                                                                                      <  1:5882483/93‑3 (MQ=255)
   gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcat                                                                                     <  1:1558936/93‑4 (MQ=255)
       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACa                                                                                 <  1:548026/93‑1 (MQ=255)
        cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                                                >  2:1621811/1‑93 (MQ=255)
         tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAgg                                                                               <  1:5263741/93‑1 (MQ=255)
          ttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGt                                                                              <  1:3309221/93‑1 (MQ=255)
            aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGa                                                                            <  1:7479604/93‑1 (MQ=255)
            aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGCCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGa                                                                            <  2:8613941/93‑1 (MQ=255)
             tCAGCGGTAAGACCGTATTCGGCGGATTCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt                                                                           <  1:7155883/93‑1 (MQ=255)
             tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt                                                                           <  1:7605950/93‑1 (MQ=255)
              cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAtt                                                                          <  2:3441582/93‑1 (MQ=255)
               aGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTg                                                                         <  2:6136218/93‑1 (MQ=255)
               aGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTg                                                                         >  1:6177898/1‑93 (MQ=255)
                gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc                                                                        <  2:3721914/93‑1 (MQ=255)
                gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc                                                                        <  2:2335810/93‑1 (MQ=255)
                gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc                                                                        <  1:35928/93‑1 (MQ=255)
                 cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcg                                                                       <  2:1693977/93‑1 (MQ=255)
                 cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcg                                                                       >  1:3449817/1‑93 (MQ=255)
                   gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcggc                                                                     <  2:4355117/93‑1 (MQ=255)
                   gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcggc                                                                     <  2:5276515/93‑1 (MQ=255)
                     aaGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGgcgc                                                                   >  2:7619970/1‑93 (MQ=255)
                      aGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCa                                                                  >  2:2202102/1‑93 (MQ=255)
                       gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCaa                                                                 >  2:6999565/1‑93 (MQ=255)
                        aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGCTTATCCTGCAGCGTCAGGCGACGCATTACAGGGGATTGCGGCGCAAt                                                                >  2:6332825/1‑93 (MQ=255)
                        aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt                                                                <  1:8638708/93‑1 (MQ=255)
                        aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt                                                                <  1:1003699/93‑1 (MQ=255)
                        aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt                                                                <  2:8146680/93‑1 (MQ=255)
                        aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt                                                                <  1:1424828/93‑1 (MQ=255)
                         ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg                                                               <  1:910431/93‑1 (MQ=255)
                         ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg                                                               >  2:2697451/1‑93 (MQ=255)
                          cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGt                                                              <  1:1286547/93‑1 (MQ=255)
                           gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGtt                                                             >  1:3983750/1‑93 (MQ=255)
                           gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGtt                                                             <  2:7774403/93‑1 (MQ=255)
                            tcttCGGCGGATACCTGACGAATGACGCGGTCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa                                                            <  1:7919428/91‑1 (MQ=255)
                            tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTACCGCGTCAGGCGACGCATTACAGGTCTTTGCGGGGCCATGTTa                                                            >  1:7272560/1‑93 (MQ=255)
                             aTTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAt                                                           <  2:385063/93‑1 (MQ=255)
                              ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAtt                                                          <  1:6243760/93‑1 (MQ=255)
                                cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCa                                                        <  2:683795/93‑1 (MQ=255)
                                 ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt                                                       <  1:442852/93‑1 (MQ=255)
                                 ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt                                                       <  1:29544/93‑1 (MQ=255)
                                  gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      >  1:2838496/1‑93 (MQ=255)
                                  gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      <  1:4275091/93‑1 (MQ=255)
                                  gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      <  1:8566415/93‑1 (MQ=255)
                                   cggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAg                                                     <  2:6180005/93‑1 (MQ=255)
                                   cggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAg                                                     <  1:2158557/93‑1 (MQ=255)
                                    ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                    <  2:4651179/93‑1 (MQ=255)
                                     gATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt                                                   >  2:659850/1‑93 (MQ=255)
                                      aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGttt                                                  >  2:7337051/1‑93 (MQ=255)
                                       tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                                                 >  1:7653582/1‑93 (MQ=255)
                                         ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc                                               <  1:3710188/93‑1 (MQ=255)
                                         ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc                                               <  2:6891389/93‑1 (MQ=255)
                                         ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc                                               <  1:4964517/93‑1 (MQ=255)
                                          cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc                                              <  2:5546812/93‑1 (MQ=255)
                                           tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                             >  2:6082103/1‑93 (MQ=255)
                                           tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                             <  1:7524221/93‑1 (MQ=255)
                                            gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCa                                            <  1:6534904/93‑1 (MQ=255)
                                            gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCa                                            >  1:3841365/1‑93 (MQ=255)
                                            gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCa                                            <  2:2976471/93‑1 (MQ=255)
                                            gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCa                                            >  1:4549703/1‑93 (MQ=255)
                                              cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                          <  1:3159895/93‑1 (MQ=255)
                                               gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata                                         >  2:7139861/1‑93 (MQ=255)
                                               gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata                                         <  2:7879055/93‑1 (MQ=255)
                                                  tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa                                      <  1:6479941/93‑1 (MQ=255)
                                                     cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaata                                   <  1:8077840/93‑1 (MQ=255)
                                                     cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaata                                   >  1:2049553/1‑93 (MQ=255)
                                                      gcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataa                                  <  2:4417579/93‑1 (MQ=255)
                                                        gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                <  2:2486480/93‑1 (MQ=255)
                                                        gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                <  1:6332826/93‑1 (MQ=255)
                                                        gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                >  1:8179795/1‑93 (MQ=255)
                                                         ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt                               >  2:3635695/1‑93 (MQ=255)
                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  1:6083453/93‑1 (MQ=255)
                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  2:3119958/93‑1 (MQ=255)
                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  1:8774208/93‑1 (MQ=255)
                                                           cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCCATAAAAATAAAGTaa                             >  1:2286627/1‑93 (MQ=255)
                                                            gATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt                            <  2:8273959/93‑1 (MQ=255)
                                                            gATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt                            <  1:688256/93‑1 (MQ=255)
                                                            gATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt                            <  1:5977662/93‑1 (MQ=255)
                                                            gATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt                            <  2:1953089/93‑1 (MQ=255)
                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          >  2:5038031/5‑93 (MQ=255)
                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  2:1337256/89‑1 (MQ=255)
                                                                 gcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                        >  2:5673090/3‑93 (MQ=255)
                                                                   aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  2:2088162/93‑1 (MQ=255)
                                                                   aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  1:5422383/93‑1 (MQ=255)
                                                                    ggATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     >  1:2778514/1‑93 (MQ=255)
                                                                    ggATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     <  2:6406877/93‑1 (MQ=255)
                                                                       ttATCCTGCAGCGTCAGGCGTCGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  <  1:6826406/93‑1 (MQ=255)
                                                                       ttATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtttt                  >  1:2618037/1‑92 (MQ=255)
                                                                        tATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGt                 >  1:1084781/1‑93 (MQ=255)
                                                                         aTCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGtt                >  2:5944457/1‑93 (MQ=255)
                                                                          tCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGttt               >  1:4786318/1‑93 (MQ=255)
                                                                               cAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAc          <  1:965986/93‑1 (MQ=255)
                                                                               cAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAc          <  1:5136058/93‑1 (MQ=255)
                                                                                 gCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCa        >  2:4917122/1‑93 (MQ=255)
                                                                                  cGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc       <  2:1146434/93‑1 (MQ=255)
                                                                                   gTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAct      >  1:5551592/1‑93 (MQ=255)
                                                                                    tCAGGCGACGCATTACAGGTGATTGAGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActc     <  2:8469840/93‑1 (MQ=255)
                                                                                    tCAGGCGACGCATTACAGGGGATTGCGGCGCAATGTTGTTCATAGTTTAACCCAATAAAAATAAAGTAAGTATACATTTGTTTAATACCActc     >  2:4743046/1‑93 (MQ=255)
                                                                                     cAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActct    <  1:1853240/93‑1 (MQ=255)
                                                                                     cAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActct    <  2:8698862/93‑1 (MQ=255)
                                                                                              |                                                                                     
GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476975‑4477153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  NC_000913/4476974‑4477161
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CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G                                                                                                <  SRR4302541.2550339/94‑1 (MQ=60)
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G                                                                                                <  SRR4302541.1593300/94‑1 (MQ=60)
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G                                                                                                <  SRR4302541.3061509/94‑1 (MQ=60)
 GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G‑C                                                                                              >  SRR4302541.3763266/1‑94 (MQ=60)
 GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G‑C                                                                                              >  SRR4302541.7280912/1‑94 (MQ=60)
 GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑G‑C                                                                                              <  SRR4302541.6238120/94‑1 (MQ=60)
 GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGCCAGGCGAC‑G‑C                                                                                              >  SRR4302541.2969616/1‑94 (MQ=60)
   TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑Gcat                                                                                             <  SRR4302541.3235248/94‑4 (MQ=60)
   TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑Gcat                                                                                             <  SRR4302541.5942345/94‑4 (MQ=60)
   TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑Gcat                                                                                             <  SRR4302541.608171/94‑4 (MQ=60)
    GTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATT                                                                                            <  SRR4302541.1574550/94‑1 (MQ=60)
    GTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATT                                                                                            <  SRR4302541.6923358/94‑1 (MQ=60)
     TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTA                                                                                           <  SRR4302541.6946234/94‑1 (MQ=60)
      AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGTTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTAC                                                                                          <  SRR4302541.8561236/94‑1 (MQ=60)
      AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTAC                                                                                          >  SRR4302541.3549616/1‑94 (MQ=60)
       TCCTTTATGAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACA                                                                                         <  SRR4302541.5246577/94‑1 (MQ=60)
       GCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACA                                                                                         >  SRR4302541.8278273/1‑94 (MQ=60)
        CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAG                                                                                        <  SRR4302541.553196/94‑1 (MQ=60)
        CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAG                                                                                        <  SRR4302541.6562857/94‑1 (MQ=60)
         CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGG                                                                                       >  SRR4302541.326905/1‑94 (MQ=60)
          TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGT                                                                                      <  SRR4302541.5317987/94‑1 (MQ=60)
          TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGT                                                                                      <  SRR4302541.2197850/94‑1 (MQ=60)
           TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTG                                                                                     <  SRR4302541.3342454/94‑1 (MQ=60)
             TTCAGCGGTAAGACCGTATTCGGCGGATCCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAT                                                                                   <  SRR4302541.6768948/94‑1 (MQ=60)
             ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAT                                                                                   <  SRR4302541.7559615/94‑1 (MQ=60)
              TCAGCGGTAAGACCGTATTCGGCGGATTCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATT                                                                                  <  SRR4302541.7232173/94‑1 (MQ=60)
              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATT                                                                                  <  SRR4302541.7232172/94‑1 (MQ=60)
              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATT                                                                                  <  SRR4302541.3980582/94‑1 (MQ=60)
              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATT                                                                                  <  SRR4302541.3499986/94‑1 (MQ=60)
              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATT                                                                                  <  SRR4302541.7687221/94‑1 (MQ=60)
              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATT                                                                                  >  SRR4302541.1769546/1‑94 (MQ=60)
               CAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTG                                                                                 <  SRR4302541.8379742/94‑1 (MQ=60)
               CAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTG                                                                                 <  SRR4302541.4317495/94‑1 (MQ=60)
               CAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTG                                                                                 <  SRR4302541.1228081/94‑1 (MQ=60)
               CAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTG                                                                                 <  SRR4302541.8379744/94‑1 (MQ=60)
               CAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTG                                                                                 >  SRR4302541.6241015/1‑94 (MQ=60)
                AGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGC                                                                                <  SRR4302541.1880280/94‑1 (MQ=60)
                 GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCG                                                                               >  SRR4302541.3484285/1‑94 (MQ=60)
                 GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCG                                                                               <  SRR4302541.6233898/94‑1 (MQ=60)
                 GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCG                                                                               <  SRR4302541.36207/94‑1 (MQ=60)
                  CGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGG                                                                              >  SRR4302541.4817278/1‑94 (MQ=60)
                  CGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGG                                                                              <  SRR4302541.5586516/94‑1 (MQ=60)
                  CGGGAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGG                                                                              <  SRR4302541.1530141/94‑1 (MQ=60)
                   GGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGC                                                                             <  SRR4302541.2892323/94‑1 (MQ=60)
                       AGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAT                                                                         >  SRR4302541.1307159/1‑94 (MQ=60)
                        GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAT                                                                        <  SRR4302541.2760952/94‑1 (MQ=60)
                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATG                                                                       <  SRR4302541.1013016/94‑1 (MQ=60)
                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATG                                                                       <  SRR4302541.7453673/94‑1 (MQ=60)
                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATG                                                                       <  SRR4302541.8731250/94‑1 (MQ=60)
                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATG                                                                       <  SRR4302541.1438695/94‑1 (MQ=60)
                          CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGT                                                                      >  SRR4302541.6013334/1‑94 (MQ=60)
                          CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGT                                                                      <  SRR4302541.918910/94‑1 (MQ=60)
                           CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTT                                                                     <  SRR4302541.1299283/94‑1 (MQ=60)
                           CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTT                                                                     <  SRR4302541.8088219/94‑1 (MQ=60)
                           CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTT                                                                     >  SRR4302541.4023232/1‑94 (MQ=60)
                           CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTT                                                                     <  SRR4302541.7012191/94‑1 (MQ=60)
                            GTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTACCGCGTCAGGCGAC‑GCATTACAGGTCTTTGCGGGGCCATGTTA                                                                    >  SRR4302541.7350151/1‑94 (MQ=57)
                             TCTTCGGCGGATACCTGACGAATGACGCGGTCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAT                                                                   <  SRR4302541.8006586/94‑1 (MQ=60)
                              ATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATT                                                                  <  SRR4302541.5037581/94‑1 (MQ=60)
                               TTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTC                                                                 <  SRR4302541.892508/94‑1 (MQ=60)
                               TTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTC                                                                 <  SRR4302541.6307545/94‑1 (MQ=60)
                                TCGGCGGATCCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCA                                                                <  SRR4302541.3841532/94‑1 (MQ=60)
                                TCGGCGGATACCTGTCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAGGTTATTCA                                                                <  SRR4302541.3841531/94‑1 (MQ=60)
                                 CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GAATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                               >  SRR4302541.983613/1‑94 (MQ=60)
                                  GGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATA                                                              <  SRR4302541.29785/94‑1 (MQ=60)
                                  GGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATA                                                              >  SRR4302541.2867053/1‑94 (MQ=60)
                                  GGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATA                                                              <  SRR4302541.447018/94‑1 (MQ=60)
                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                             <  SRR4302541.8186947/94‑1 (MQ=60)
                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                             <  SRR4302541.8658434/94‑1 (MQ=60)
                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                             <  SRR4302541.4511045/94‑1 (MQ=60)
                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                             <  SRR4302541.4317799/94‑1 (MQ=60)
                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                             <  SRR4302541.8797042/94‑1 (MQ=60)
                                    CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                                                            <  SRR4302541.6662095/94‑1 (MQ=60)
                                    CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                                                            <  SRR4302541.2179427/94‑1 (MQ=60)
                                    CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                                                            >  SRR4302541.433722/1‑94 (MQ=60)
                                    CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTAAGGGGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATCGT                                                            >  SRR4302541.2628987/1‑94 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                           <  SRR4302541.7698293/94‑1 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                           <  SRR4302541.5292744/94‑1 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                           >  SRR4302541.5256659/1‑94 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                           <  SRR4302541.3390864/94‑1 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                           <  SRR4302541.2083514/94‑1 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                           >  SRR4302541.667991/1‑94 (MQ=60)
                                     GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGGT                                                           >  SRR4302541.8114026/1‑94 (MQ=60)
                                      GATACCTGACGAATGACGGGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTG‑GGCGCAATGTTATTCATAGTTTA                                                         <  SRR4302541.5398128/94‑1 (MQ=60)
                                      GATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTT                                                          <  SRR4302541.3648821/94‑1 (MQ=60)
                                       ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                         <  SRR4302541.8720173/94‑1 (MQ=60)
                                       ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                         >  SRR4302541.7735100/1‑94 (MQ=60)
                                        TACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAA                                                        >  SRR4302541.6507842/1‑94 (MQ=60)
                                        TACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAA                                                        <  SRR4302541.7075269/94‑1 (MQ=60)
                                         ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                       <  SRR4302541.479672/94‑1 (MQ=60)
                                         ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                       <  SRR4302541.8730389/94‑1 (MQ=60)
                                          CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                                                      <  SRR4302541.3747630/94‑1 (MQ=60)
                                          CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                                                      <  SRR4302541.5015349/94‑1 (MQ=60)
                                          CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                                                      <  SRR4302541.7708939/94‑1 (MQ=60)
                                           CTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC                                                     >  SRR4302541.3108359/1‑94 (MQ=60)
                                           CTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC                                                     <  SRR4302541.5555867/94‑1 (MQ=60)
                                           CTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC                                                     >  SRR4302541.3108360/1‑94 (MQ=60)
                                            TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                                                    >  SRR4302541.4595726/1‑94 (MQ=60)
                                            TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                                                    <  SRR4302541.7604619/94‑1 (MQ=60)
                                            TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                                                    >  SRR4302541.3880055/1‑94 (MQ=60)
                                             GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAA                                                   >  SRR4302541.2589796/1‑94 (MQ=60)
                                             GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAA                                                   <  SRR4302541.6601633/94‑1 (MQ=60)
                                             GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGAGTCAGGCGAC‑GCATTACAGGTGAATGAGGCGCAATGTTATTCATAGTTTAAacaaa                                                   >  SRR4302541.2589797/1‑89 (MQ=60)
                                              ACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAAT                                                  <  SRR4302541.5461034/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                                 <  SRR4302541.3191805/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                                 <  SRR4302541.1438218/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                                 <  SRR4302541.975049/94‑1 (MQ=60)
                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                                 >  SRR4302541.7953806/1‑94 (MQ=60)
                                                GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAA                                                >  SRR4302541.6362569/1‑94 (MQ=60)
                                                GAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAA                                                <  SRR4302541.6599871/94‑1 (MQ=60)
                                                   TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                             <  SRR4302541.6545981/94‑1 (MQ=60)
                                                    GACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAAT                                            <  SRR4302541.451471/94‑1 (MQ=60)
                                                    GACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAAT                                            <  SRR4302541.2851929/94‑1 (MQ=60)
                                                     ACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATA                                           >  SRR4302541.2069333/1‑94 (MQ=60)
                                                     ACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATA                                           <  SRR4302541.592052/94‑1 (MQ=60)
                                                      CGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAA                                          >  SRR4302541.1234013/1‑94 (MQ=60)
                                                      CGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAA                                          <  SRR4302541.3391551/94‑1 (MQ=60)
                                                      CGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAA                                          <  SRR4302541.8166465/94‑1 (MQ=60)
                                                       GCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAA                                         <  SRR4302541.548331/94‑1 (MQ=60)
                                                       GCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAA                                         <  SRR4302541.2716617/94‑1 (MQ=60)
                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                        <  SRR4302541.7238778/94‑1 (MQ=60)
                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                        >  SRR4302541.8269103/1‑94 (MQ=60)
                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                        <  SRR4302541.1890349/94‑1 (MQ=60)
                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                        <  SRR4302541.596916/94‑1 (MQ=60)
                                                         GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGT                                       <  SRR4302541.6397572/94‑1 (MQ=60)
                                                         GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGT                                       <  SRR4302541.6268622/94‑1 (MQ=60)
                                                         GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGT                                       <  SRR4302541.4968943/94‑1 (MQ=60)
                                                          cgggtatagcAGGATTATCCGGCAGCGTCAGGCGAC‑GCAGTACAGGTGATTGCGGCGCAAGGTTATTCATAGTTTAACCCAATAAAAATAAAGT                                       <  SRR4302541.6397571/84‑1 (MQ=60)
                                                          GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                      >  SRR4302541.6313105/1‑94 (MQ=60)
                                                          GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                      >  SRR4302541.8829479/1‑94 (MQ=60)
                                                           GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCCATAAAAATAAAGTAA                                     >  SRR4302541.2308758/1‑94 (MQ=60)
                                                           GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                     <  SRR4302541.6145548/94‑1 (MQ=60)
                                                           GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                     <  SRR4302541.8868000/94‑1 (MQ=60)
                                                            CGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT                                    <  SRR4302541.3958277/94‑1 (MQ=60)
                                                             GATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT                                   <  SRR4302541.6038749/94‑1 (MQ=60)
                                                             GATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT                                   <  SRR4302541.694735/94‑1 (MQ=60)
                                                              ATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA                                  <  SRR4302541.6233422/94‑1 (MQ=60)
                                                                   CAGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACA                              <  SRR4302541.7580139/94‑1 (MQ=60)
                                                                   CAGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACA                              <  SRR4302541.4044001/94‑1 (MQ=60)
                                                                    AGGATTATCCTGCAGCGTCAGGCGACTC‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                             <  SRR4302541.7805446/94‑1 (MQ=60)
                                                                    AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                             >  SRR4302541.7832152/1‑94 (MQ=60)
                                                                    AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                             >  SRR4302541.3722884/1‑94 (MQ=60)
                                                                    AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                             <  SRR4302541.5478015/94‑1 (MQ=60)
                                                                    AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                             >  SRR4302541.2806516/1‑94 (MQ=60)
                                                                     GGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                            >  SRR4302541.1350031/1‑94 (MQ=60)
                                                                      GATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                           <  SRR4302541.8177995/94‑1 (MQ=60)
                                                                      GATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                           >  SRR4302541.6125287/1‑94 (MQ=60)
                                                                      GATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                           >  SRR4302541.6125289/1‑94 (MQ=60)
                                                                       ATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTT                          >  SRR4302541.2644181/1‑94 (MQ=60)
                                                                       ATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTG                          >  SRR4302541.6288991/1‑94 (MQ=60)
                                                                       ATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTG                          <  SRR4302541.4030950/94‑1 (MQ=60)
                                                                       ATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTG                          <  SRR4302541.5770398/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGTC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                         <  SRR4302541.6899453/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                         <  SRR4302541.1766565/94‑1 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                         >  SRR4302541.1094913/1‑94 (MQ=60)
                                                                        TTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                         >  SRR4302541.1094914/1‑94 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                        <  SRR4302541.7589309/94‑1 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                        >  SRR4302541.550479/1‑94 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                        <  SRR4302541.7589310/94‑1 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                        >  SRR4302541.3372525/1‑94 (MQ=60)
                                                                         TATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                        >  SRR4302541.6416390/1‑94 (MQ=60)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTT                       >  SRR4302541.4835157/1‑94 (MQ=60)
                                                                          ATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTT                       <  SRR4302541.1062629/94‑1 (MQ=60)
                                                                           TCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTA                      >  SRR4302541.6316085/1‑94 (MQ=60)
                                                                           TCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTA                      <  SRR4302541.96768/94‑1 (MQ=60)
                                                                             CTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAT                    <  SRR4302541.4989166/94‑1 (MQ=60)
                                                                              TGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATA                   <  SRR4302541.7629249/94‑1 (MQ=60)
                                                                               GCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAC                  <  SRR4302541.2482565/94‑1 (MQ=60)
                                                                               GCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAC                  >  SRR4302541.1455710/1‑94 (MQ=60)
                                                                                CAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACC                 <  SRR4302541.5188870/94‑1 (MQ=60)
                                                                                CAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACC                 <  SRR4302541.975063/94‑1 (MQ=60)
                                                                                 AGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAACTAAAGTAATTATACATTTGTTTAATACCA                >  SRR4302541.4451877/1‑94 (MQ=60)
                                                                                 AGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCA                >  SRR4302541.5082463/1‑94 (MQ=60)
                                                                                  GCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAC               >  SRR4302541.7609841/1‑94 (MQ=60)
                                                                                   CGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT              <  SRR4302541.2914672/94‑1 (MQ=60)
                                                                                   CGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT              >  SRR4302541.5600181/1‑94 (MQ=60)
                                                                                   CGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT              >  SRR4302541.5608425/1‑94 (MQ=60)
                                                                                   CGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT              >  SRR4302541.898875/1‑94 (MQ=60)
                                                                                   CGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT              >  SRR4302541.898874/1‑94 (MQ=60)
                                                                                    GTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTC             <  SRR4302541.4639752/94‑1 (MQ=60)
                                                                                    GTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTC             <  SRR4302541.2156854/94‑1 (MQ=60)
                                                                                     TCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT            <  SRR4302541.1903164/94‑1 (MQ=60)
                                                                                      CAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTC           <  SRR4302541.7360769/94‑1 (MQ=60)
                                                                                      CAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTC           <  SRR4302541.1871147/94‑1 (MQ=60)
                                                                                      CAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTC           >  SRR4302541.2217361/1‑94 (MQ=60)
                                                                                        GGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT         <  SRR4302541.2834201/94‑1 (MQ=60)
                                                                                          CGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACNCAATAAAAATAAAGTAATTATACNNNNGTNNAATNCCNCNNTNTNNN       >  SRR4302541.6626946/1‑94 (MQ=60)
                                                                                          CGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCT       >  SRR4302541.3558953/1‑94 (MQ=60)
                                                                                           GAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT      <  SRR4302541.1803941/94‑1 (MQ=60)
                                                                                           GAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT      <  SRR4302541.6940076/94‑1 (MQ=60)
                                                                                            AC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA     <  SRR4302541.3844030/94‑1 (MQ=60)
                                                                                               C‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  SRR4302541.5002123/1‑94 (MQ=60)
                                                                                               C‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  SRR4302541.1577062/1‑94 (MQ=60)
                                                                                               |                                                                                              
CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  NC_000913/4476974‑4477161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑