Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,319,717 A→G noncoding (83/87 nt) leuU ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,319,7170AG99.2% 914.0 / ‑3.5 268noncoding (83/87 nt)leuUtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (141/125);  total (142/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 273 total aligned reads displayed.

GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806
                                                                                          |                                                                                         
ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGcc                                                                                         <  2:915364/93‑1 (MQ=255)
ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGcc                                                                                         <  2:5153914/93‑1 (MQ=255)
ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGcc                                                                                         <  1:3151637/93‑1 (MQ=255)
 gACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCg                                                                                        >  2:3961197/1‑93 (MQ=255)
     agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGa                                                                                    >  1:795951/1‑93 (MQ=255)
      gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAc                                                                                   <  2:2472971/93‑1 (MQ=255)
         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACggg                                                                                <  1:403336/93‑1 (MQ=255)
         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACggg                                                                                <  2:780858/93‑1 (MQ=255)
           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              <  1:1447828/93‑1 (MQ=255)
           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              <  1:675250/93‑1 (MQ=255)
           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              >  1:1889323/1‑93 (MQ=255)
            gatacCTGACACCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             <  1:1988430/93‑1 (MQ=255)
            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             <  2:3578359/93‑1 (MQ=255)
            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             >  2:291627/1‑93 (MQ=255)
            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             <  2:5204969/93‑1 (MQ=255)
               acCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGa                                                                          <  2:6424130/93‑1 (MQ=255)
                ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGaa                                                                         >  2:3005750/1‑93 (MQ=255)
                ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGaa                                                                         <  1:2592880/93‑1 (MQ=255)
                 cTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAc                                                                        >  1:1243777/1‑93 (MQ=255)
                 cTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAc                                                                        <  1:5394260/93‑1 (MQ=255)
                  tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAcc                                                                       <  2:2447699/93‑1 (MQ=255)
                   gACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAccc                                                                      >  1:520010/1‑93 (MQ=255)
                   gACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAccc                                                                      <  2:1094158/93‑1 (MQ=255)
                    aCAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCg                                                                     <  2:4930194/93‑1 (MQ=255)
                     cAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGt                                                                    >  1:1058103/1‑93 (MQ=255)
                      aaCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTa                                                                   <  2:138500/93‑1 (MQ=255)
                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  1:1560845/1‑93 (MQ=255)
                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  1:3857003/1‑93 (MQ=255)
                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  2:2219621/1‑93 (MQ=255)
                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACGTGCACCCGTaa                                                                  >  1:2233525/1‑93 (MQ=255)
                        ccATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAg                                                                 >  1:2820108/1‑93 (MQ=255)
                         cATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGc                                                                >  1:4113224/1‑93 (MQ=255)
                          aTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGcc                                                               >  1:1261202/1‑93 (MQ=255)
                          aTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGcc                                                               >  1:3396643/1‑93 (MQ=255)
                           ttCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGccc                                                              <  1:2608779/93‑1 (MQ=255)
                            tCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCt                                                             <  1:1159527/93‑1 (MQ=255)
                            tCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCt                                                             >  1:5150499/1‑93 (MQ=255)
                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            >  2:4308452/1‑93 (MQ=255)
                                cAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                         >  2:2799180/1‑93 (MQ=255)
                                cAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                         <  2:4811355/93‑1 (MQ=255)
                                 aGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                                        <  2:2738903/93‑1 (MQ=255)
                                   aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                      <  2:1796509/93‑1 (MQ=255)
                                    ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCa                                                     <  2:1224755/93‑1 (MQ=255)
                                     cAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAACCGTAAGCCCTATTGGGCAc                                                    >  1:1186279/1‑93 (MQ=255)
                                      aGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACt                                                   >  2:4718817/1‑93 (MQ=255)
                                      aGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACt                                                   <  1:5021787/93‑1 (MQ=255)
                                       ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                  <  2:4562385/93‑1 (MQ=255)
                                          ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccc                                               >  2:3873193/1‑92 (MQ=255)
                                           cAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                              <  1:3553398/93‑1 (MQ=255)
                                            aaaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                             >  1:2658973/1‑93 (MQ=255)
                                              aaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTc                                           >  2:252174/1‑93 (MQ=255)
                                               aCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCa                                          <  1:3884041/93‑1 (MQ=255)
                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         <  2:2995594/93‑1 (MQ=255)
                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         >  2:6335674/1‑93 (MQ=255)
                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         <  1:2103619/93‑1 (MQ=255)
                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACCTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         >  1:2902514/1‑93 (MQ=255)
                                                   aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGt                                      <  2:6275698/93‑1 (MQ=255)
                                                   aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGt                                      <  2:4459642/93‑1 (MQ=255)
                                                    aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTa                                     <  2:6025759/93‑1 (MQ=255)
                                                     aaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAg                                    >  1:1358084/1‑93 (MQ=255)
                                                      aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGc                                   <  1:3296200/93‑1 (MQ=255)
                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  1:6124701/1‑93 (MQ=255)
                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  2:2583996/1‑93 (MQ=255)
                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATCGGGCACTACCACCTCAAGGTAGCg                                  <  1:6224245/93‑1 (MQ=255)
                                                        gACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                 <  1:2500538/93‑1 (MQ=255)
                                                         aCGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtg                                >  2:4534543/1‑93 (MQ=255)
                                                          cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtgt                               >  2:2000515/1‑93 (MQ=255)
                                                          cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtgt                               <  1:5621868/93‑1 (MQ=255)
                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              <  1:2408067/93‑1 (MQ=255)
                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              >  2:3240653/1‑93 (MQ=255)
                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              <  1:15448/93‑1 (MQ=255)
                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              >  1:4833658/1‑93 (MQ=255)
                                                                tCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCa                         <  1:1931691/93‑1 (MQ=255)
                                                                tCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCa                         <  2:4913520/93‑1 (MQ=255)
                                                                 cAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCaa                        >  2:858554/1‑93 (MQ=255)
                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      >  2:1703636/1‑93 (MQ=255)
                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      >  2:5620264/1‑93 (MQ=255)
                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      <  2:4166359/93‑1 (MQ=255)
                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      >  1:461799/1‑93 (MQ=255)
                                                                     gTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                    >  1:1696536/1‑93 (MQ=255)
                                                                     gTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                    <  2:379028/93‑1 (MQ=255)
                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  1:3178037/1‑93 (MQ=255)
                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  1:5513442/1‑93 (MQ=255)
                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  2:3342078/1‑93 (MQ=255)
                                                                        tctTTTCTGGAATTTGGTGACGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccaca                 >  2:709417/1‑92 (MQ=255)
                                                                          ttttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCcaccac               >  2:1625874/1‑93 (MQ=255)
                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             <  2:5460090/93‑1 (MQ=255)
                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             <  1:4198423/93‑1 (MQ=255)
                                                                             tCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTc            <  1:5753644/93‑1 (MQ=255)
                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           >  1:2317811/1‑93 (MQ=255)
                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           <  2:6117878/93‑1 (MQ=255)
                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           <  1:743651/93‑1 (MQ=255)
                                                                               tGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCgg          >  2:1885580/1‑93 (MQ=255)
                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         <  1:965632/93‑1 (MQ=255)
                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  2:3039026/1‑93 (MQ=255)
                                                                                   aTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACg      >  1:6424129/1‑93 (MQ=255)
                                                                                    tttGGTGCCGAGGACGGGACTTGAACCCGTAAGACCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACAACCTCCGCACgg     >  1:3745036/1‑93 (MQ=255)
                                                                                      tGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACTGAt   <  1:3979224/93‑1 (MQ=255)
                                                                                      tGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt   <  2:1133833/93‑1 (MQ=255)
                                                                                      tGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt   >  1:5912519/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACT  >  NC_000913/3319624‑3319808
                                                                                             |                                                                                           
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTG                                                                                             <  SRR4302534.3701145/94‑1 (MQ=60)
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTG                                                                                             <  SRR4302534.750290/94‑1 (MQ=60)
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTA                                                                                             <  SRR4302534.4936481/94‑1 (MQ=60)
  TGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCC                                                                                           >  SRR4302534.2646950/1‑94 (MQ=60)
   GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGTATTTGGTGCCG                                                                                          >  SRR4302534.2213711/1‑94 (MQ=60)
   GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCG                                                                                          >  SRR4302534.662351/1‑94 (MQ=60)
   GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCG                                                                                          <  SRR4302534.3234611/94‑1 (MQ=60)
   GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCG                                                                                          >  SRR4302534.1473851/1‑94 (MQ=60)
   GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCG                                                                                          <  SRR4302534.4601648/94‑1 (MQ=60)
    GACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGA                                                                                         >  SRR4302534.1862940/1‑94 (MQ=60)
      CGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGG                                                                                       <  SRR4302534.1278573/94‑1 (MQ=60)
       GAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGA                                                                                      >  SRR4302534.55308/1‑94 (MQ=60)
       GAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGA                                                                                      <  SRR4302534.249081/94‑1 (MQ=60)
       GAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGA                                                                                      >  SRR4302534.815926/1‑94 (MQ=60)
       GAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGA                                                                                      >  SRR4302534.2945896/1‑94 (MQ=60)
       GAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGA                                                                                      >  SRR4302534.1993303/1‑94 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                     >  SRR4302534.281775/1‑94 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                     <  SRR4302534.3118070/94‑1 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                     <  SRR4302534.119341/94‑1 (MQ=60)
         GATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACG                                                                                    >  SRR4302534.5753827/1‑94 (MQ=60)
           TACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGG                                                                                  <  SRR4302534.1685398/94‑1 (MQ=60)
           TACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACCGG                                                                                  <  SRR4302534.4163160/94‑1 (MQ=60)
            ACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGA                                                                                 >  SRR4302534.6022883/1‑94 (MQ=60)
            ACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGA                                                                                 <  SRR4302534.413646/94‑1 (MQ=60)
             CAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAC                                                                                >  SRR4302534.1938590/1‑94 (MQ=60)
              AGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACT                                                                               >  SRR4302534.5896014/1‑94 (MQ=60)
              AGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACT                                                                               <  SRR4302534.691715/94‑1 (MQ=60)
              AGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACT                                                                               <  SRR4302534.1485928/94‑1 (MQ=60)
               GATACCTGACACCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTT                                                                              <  SRR4302534.2040493/94‑1 (MQ=60)
               GATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTT                                                                              >  SRR4302534.3713057/1‑94 (MQ=60)
                 TACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCGGAGGACGGGACTTGA                                                                            <  SRR4302534.4028165/94‑1 (MQ=60)
                 TACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGA                                                                            >  SRR4302534.2680082/1‑94 (MQ=60)
                  ACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAA                                                                           <  SRR4302534.3992409/94‑1 (MQ=60)
                   CCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAC                                                                          >  SRR4302534.1276562/1‑94 (MQ=60)
                   CCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAC                                                                          <  SRR4302534.2661510/94‑1 (MQ=60)
                    CTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACC                                                                         <  SRR4302534.5539504/94‑1 (MQ=60)
                     TGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCC                                                                        >  SRR4302534.532939/1‑94 (MQ=60)
                      GACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCG                                                                       >  SRR4302534.344940/1‑94 (MQ=60)
                       ACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGT                                                                      >  SRR4302534.1085507/1‑94 (MQ=60)
                       ACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAAACGCTTTTCAGCGTCTCTTTTCTGGAAATTGATGCCGAGGACGGCGCTTGAACTCGT                                                                      >  SRR4302534.5883437/1‑94 (MQ=60)
                        CAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTA                                                                     >  SRR4302534.6333159/1‑94 (MQ=60)
                         AACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAA                                                                    >  SRR4302534.1601701/1‑94 (MQ=60)
                         AACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAA                                                                    >  SRR4302534.3767540/1‑94 (MQ=60)
                         AACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAA                                                                    >  SRR4302534.3960549/1‑94 (MQ=60)
                         AACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACGTGCACCCGTAA                                                                    >  SRR4302534.2292689/1‑94 (MQ=60)
                          ACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGTGACTTGAACCCGTAAG                                                                   <  SRR4302534.777380/94‑1 (MQ=60)
                          ACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAG                                                                   >  SRR4302534.2894264/1‑94 (MQ=60)
                           CCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGC                                                                  >  SRR4302534.4223876/1‑94 (MQ=60)
                           CCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGC                                                                  >  SRR4302534.5246062/1‑94 (MQ=60)
                            CATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCC                                                                 >  SRR4302534.3487793/1‑94 (MQ=60)
                            CATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCC                                                                 >  SRR4302534.1294426/1‑94 (MQ=60)
                             ATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCC                                                                >  SRR4302534.2895406/1‑94 (MQ=60)
                              TTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCT                                                               <  SRR4302534.1977741/94‑1 (MQ=60)
                              TTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCT                                                               <  SRR4302534.3582643/94‑1 (MQ=60)
                              TTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCT                                                               >  SRR4302534.5289446/1‑94 (MQ=60)
                              TTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCT                                                               <  SRR4302534.2677805/94‑1 (MQ=60)
                               TCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTA                                                              <  SRR4302534.1189951/94‑1 (MQ=60)
                                CCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAT                                                             >  SRR4302534.5694583/1‑94 (MQ=60)
                                 CTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATT                                                            <  SRR4302534.1555167/94‑1 (MQ=60)
                                  TCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTG                                                           <  SRR4302534.601188/94‑1 (MQ=60)
                                   CAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGG                                                          >  SRR4302534.1586970/1‑94 (MQ=60)
                                   CAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGG                                                          <  SRR4302534.697886/94‑1 (MQ=60)
                                   CAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGG                                                          >  SRR4302534.68393/1‑94 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                         <  SRR4302534.272083/94‑1 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                         >  SRR4302534.5150826/1‑94 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                         <  SRR4302534.1204168/94‑1 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                         >  SRR4302534.969958/1‑94 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                         <  SRR4302534.5424538/94‑1 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                         <  SRR4302534.814145/94‑1 (MQ=60)
                                     GACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGC                                                        <  SRR4302534.6546251/94‑1 (MQ=60)
                                      ACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCA                                                       >  SRR4302534.253976/1‑94 (MQ=60)
                                       CCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAC                                                      >  SRR4302534.4232584/1‑94 (MQ=60)
                                       CCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAACCGTAAGCCCTATTGGGCAC                                                      >  SRR4302534.1217473/1‑94 (MQ=60)
                                       CCAGGACCAAAACGAAAAAAGACGATTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAC                                                      >  SRR4302534.5323950/1‑94 (MQ=60)
                                        CAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACT                                                     <  SRR4302534.3970935/94‑1 (MQ=60)
                                         AGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTA                                                    <  SRR4302534.5860350/94‑1 (MQ=60)
                                         AGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTA                                                    >  SRR4302534.1357779/1‑94 (MQ=60)
                                         AGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTA                                                    <  SRR4302534.5157453/94‑1 (MQ=60)
                                         AGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTA                                                    >  SRR4302534.1523577/1‑94 (MQ=60)
                                           GACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACC                                                  >  SRR4302534.2103323/1‑94 (MQ=60)
                                            ACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCA                                                 >  SRR4302534.5543870/1‑94 (MQ=60)
                                            ACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCA                                                 >  SRR4302534.1407894/1‑94 (MQ=60)
                                             CCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCAC                                                <  SRR4302534.3900289/94‑1 (MQ=60)
                                             CCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCAC                                                >  SRR4302534.3595881/1‑94 (MQ=60)
                                              CAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGACTTTGGTGCCGAGGACGGGACTTGAACACGTAAGCCCTATTGGGCACTcacaac                                               >  SRR4302534.4212280/1‑88 (MQ=60)
                                              CAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACC                                               <  SRR4302534.3648960/94‑1 (MQ=60)
                                              CAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACC                                               >  SRR4302534.2729271/1‑94 (MQ=60)
                                               AAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCT                                              <  SRR4302534.3242357/94‑1 (MQ=60)
                                                 AACGAAAAAAGACGCTTTTGCGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCGCTATTGGGCACTgcgccctca                                            >  SRR4302534.1996969/1‑85 (MQ=60)
                                                 AACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCA                                            <  SRR4302534.262191/94‑1 (MQ=60)
                                                  ACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGGCTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAA                                           >  SRR4302534.4725666/1‑94 (MQ=60)
                                                  ACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAA                                           <  SRR4302534.3988314/94‑1 (MQ=60)
                                                  ACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACCTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAA                                           >  SRR4302534.2978681/1‑94 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGGCCTATTGGGCACTACCACCTCAAG                                          >  SRR4302534.1138677/1‑94 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAG                                          >  SRR4302534.4099718/1‑94 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAG                                          <  SRR4302534.2158851/94‑1 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAG                                          >  SRR4302534.2330685/1‑94 (MQ=60)
                                                    GAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGG                                         >  SRR4302534.6328993/1‑94 (MQ=60)
                                                     AAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGT                                        >  SRR4302534.6284744/1‑94 (MQ=60)
                                                       AAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAG                                      >  SRR4302534.1393869/1‑94 (MQ=60)
                                                         AAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCG                                    <  SRR4302534.3384132/94‑1 (MQ=60)
                                                         AAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCG                                    >  SRR4302534.6291290/1‑94 (MQ=60)
                                                          AGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGT                                   >  SRR4302534.6527137/1‑94 (MQ=60)
                                                          AGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGT                                   >  SRR4302534.3254907/1‑94 (MQ=60)
                                                          AGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGT                                   <  SRR4302534.2043210/94‑1 (MQ=60)
                                                          AGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATCGGGCACTACCACCTCAAGGTAGCGT                                   <  SRR4302534.6393251/94‑1 (MQ=60)
                                                           GACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTG                                  <  SRR4302534.1258472/94‑1 (MQ=60)
                                                           GACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTG                                  <  SRR4302534.2566930/94‑1 (MQ=60)
                                                           GACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGCGCCGAGGACGGGACTTGAACCCGCAAGCCCTATTTGGAACTACCACCTCAAGGTAGCGTG                                  >  SRR4302534.4987489/1‑94 (MQ=60)
                                                            ACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGT                                 <  SRR4302534.4753126/94‑1 (MQ=60)
                                                             CGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTC                                <  SRR4302534.5773995/94‑1 (MQ=60)
                                                             CGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTC                                >  SRR4302534.4964806/1‑94 (MQ=60)
                                                             CGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTC                                >  SRR4302534.2674792/1‑94 (MQ=60)
                                                              GCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCT                               <  SRR4302534.15864/94‑1 (MQ=60)
                                                              GCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCT                               <  SRR4302534.2472073/94‑1 (MQ=60)
                                                              CCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCT                               <  SRR4302534.194213/94‑1 (MQ=60)
                                                               CTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAATTGAAACCGTAAACCCCATTGGGCACTCCCACCCCAAGGTAGCGTGGCTA                              >  SRR4302534.4500436/1‑94 (MQ=60)
                                                                TTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAC                             <  SRR4302534.227179/94‑1 (MQ=60)
                                                                  TTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCA                           >  SRR4302534.1629938/1‑94 (MQ=60)
                                                                   TCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAA                          <  SRR4302534.1982195/94‑1 (MQ=60)
                                                                   TCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAA                          >  SRR4302534.3265730/1‑94 (MQ=60)
                                                                   TCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAA                          <  SRR4302534.3272812/94‑1 (MQ=60)
                                                                     AGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATT                        >  SRR4302534.4103727/1‑94 (MQ=60)
                                                                     AGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATT                        >  SRR4302534.473460/1‑94 (MQ=60)
                                                                       CGTCTCTTTTCTGGAATTTTGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCC                      >  SRR4302534.4931160/1‑94 (MQ=60)
                                                                       CGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTCAGCCCTATTGGGCACTACCACCCCAAGGTAGCGTGTCTACCAATTCC                      <  SRR4302534.5322325/94‑1 (MQ=60)
                                                                       CGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCC                      >  SRR4302534.419344/1‑94 (MQ=60)
                                                                       CGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCC                      >  SRR4302534.1740625/1‑94 (MQ=60)
                                                                        GTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCA                     >  SRR4302534.3261964/1‑94 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                   >  SRR4302534.5662177/1‑94 (MQ=60)
                                                                             TTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACC                <  SRR4302534.1335528/94‑1 (MQ=60)
                                                                               TTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTC              <  SRR4302534.3877253/94‑1 (MQ=60)
                                                                               TTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTC              <  SRR4302534.932495/94‑1 (MQ=60)
                                                                               TTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTC              <  SRR4302534.4311813/94‑1 (MQ=60)
                                                                                TCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG             <  SRR4302534.5909688/94‑1 (MQ=60)
                                                                                TCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG             >  SRR4302534.2379319/1‑94 (MQ=60)
                                                                                TCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG             <  SRR4302534.1258347/94‑1 (MQ=60)
                                                                                 CTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGG            <  SRR4302534.762077/94‑1 (MQ=60)
                                                                                   GGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCA          <  SRR4302534.990243/94‑1 (MQ=60)
                                                                                   GGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCA          <  SRR4302534.2385759/94‑1 (MQ=60)
                                                                                    GAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAC         >  SRR4302534.1786745/1‑94 (MQ=60)
                                                                                     AATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACG        >  SRR4302534.6597380/1‑94 (MQ=60)
                                                                                      ATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGG       <  SRR4302534.51397/94‑1 (MQ=60)
                                                                                      ATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGACCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACAACCTCCGCACGG       >  SRR4302534.3845643/1‑94 (MQ=60)
                                                                                        TTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAT     >  SRR4302534.6073075/1‑94 (MQ=60)
                                                                                         TGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACTGATA    <  SRR4302534.4085709/94‑1 (MQ=60)
                                                                                         TGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA    <  SRR4302534.2450500/94‑1 (MQ=60)
                                                                                          GGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATAC   <  SRR4302534.277764/94‑1 (MQ=60)
                                                                                          GGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATAC   >  SRR4302534.2944227/1‑94 (MQ=60)
                                                                                          GGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCCTCACGGATAC   >  SRR4302534.4606364/1‑94 (MQ=60)
                                                                                           GTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACT  <  SRR4302534.3561954/94‑1 (MQ=60)
                                                                                             |                                                                                           
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACT  >  NC_000913/3319624‑3319808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑