Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,352,254 2 bp→GC intergenic (‑569/‑12) yhcC ← / → gltB hypothetical protein/glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,2540CG100.0% 803.0 / NA 228intergenic (‑569/‑13)yhcC/gltBhypothetical protein/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (144/84);  total (144/84)
*NC_0009133,352,2550GC100.0% 803.5 / NA 228intergenic (‑570/‑12)yhcC/gltBhypothetical protein/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (144/84);  total (144/84)

Only 100 of 232 total aligned reads displayed.

AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGC  >  NC_000913/3352167‑3352341
                                                                                       ||                                                                                      
agcagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAgg                                                                                    >  2:6077307/1‑93 (MQ=255)
 gcagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgc                                                                                   >  2:5585515/1‑93 (MQ=255)
    gcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg                                                                                >  2:4168435/1‑93 (MQ=255)
      cTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGcgcgcg                                                                              >  1:4593710/1‑93 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt                                                                             >  2:1221479/1‑93 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt                                                                             >  1:540936/1‑93 (MQ=255)
        gCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTa                                                                            <  2:867548/93‑1 (MQ=255)
        gCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTa                                                                            >  2:3307133/1‑93 (MQ=255)
        gCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTa                                                                            <  1:4845392/93‑1 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGTGGCGAATGCGAGGCGCGCGTATg                                                                          >  1:5230308/1‑93 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                          >  1:4038673/1‑93 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                          <  1:2731595/93‑1 (MQ=255)
           catcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGa                                                                         >  1:1248625/1‑93 (MQ=255)
            atcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGac                                                                        >  1:2817711/1‑93 (MQ=255)
            atcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGac                                                                        >  1:1405828/1‑93 (MQ=255)
              catATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGacac                                                                      <  1:2250675/93‑1 (MQ=255)
                tatTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc                                                                    >  1:4738780/1‑93 (MQ=255)
                 atTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCa                                                                   >  2:2645592/1‑93 (MQ=255)
                  tttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaa                                                                  >  2:688156/1‑93 (MQ=255)
                  tttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaa                                                                  >  2:2327544/1‑93 (MQ=255)
                    tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAc                                                                <  2:1365574/93‑1 (MQ=255)
                      tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc                                                              >  1:6394758/1‑93 (MQ=255)
                       gCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc                                                             >  1:2389426/1‑93 (MQ=255)
                        cAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCg                                                            <  2:3256372/93‑1 (MQ=255)
                         aGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGt                                                           <  1:1308311/93‑1 (MQ=255)
                         aGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGt                                                           >  2:1589566/1‑93 (MQ=255)
                           tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCg                                                         <  1:3241367/93‑1 (MQ=255)
                            aaTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                        >  2:4899321/1‑93 (MQ=255)
                               tGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc                                                     <  1:4484350/93‑1 (MQ=255)
                                 agaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGc                                                   <  2:3596143/93‑1 (MQ=255)
                                  gaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACATCAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCACACCCCGTCTCCACGct                                                  >  2:4687507/1‑93 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 >  2:2235598/1‑93 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 >  2:2445460/1‑93 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 <  1:5961344/93‑1 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 <  1:3126097/93‑1 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 >  1:5502015/1‑93 (MQ=255)
                                    tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctct                                                <  1:749118/93‑1 (MQ=255)
                                    tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctct                                                <  2:3130030/93‑1 (MQ=255)
                                     ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt                                               <  1:6238245/93‑1 (MQ=255)
                                      cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt                                              >  2:107615/1‑93 (MQ=255)
                                       ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc                                             >  2:3736309/1‑93 (MQ=255)
                                        ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt                                            >  1:5528364/1‑93 (MQ=255)
                                         tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                           <  1:926112/93‑1 (MQ=255)
                                          ctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                          <  1:1842307/93‑1 (MQ=255)
                                          ctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                          <  1:6407352/93‑1 (MQ=255)
                                           ttCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgtg                                         >  1:870252/1‑93 (MQ=255)
                                           ttCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgtg                                         >  2:2069871/1‑93 (MQ=255)
                                            tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGc                                        >  1:5780856/1‑93 (MQ=255)
                                             cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc                                       >  1:1938663/1‑93 (MQ=255)
                                              aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc                                      >  2:2881956/1‑93 (MQ=255)
                                              aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc                                      >  2:5823839/1‑93 (MQ=255)
                                                cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt                                    >  1:4770960/1‑93 (MQ=255)
                                                 gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg                                   >  2:4631843/1‑93 (MQ=255)
                                                 gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg                                   <  2:5172025/93‑1 (MQ=255)
                                                 gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg                                   <  1:3381869/93‑1 (MQ=255)
                                                  tATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc                                  <  1:5054635/93‑1 (MQ=255)
                                                  tATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc                                  >  2:5480270/1‑93 (MQ=255)
                                                   aTTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTccg                                 >  2:5387951/1‑93 (MQ=255)
                                                     tAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCa                               <  1:4371002/93‑1 (MQ=255)
                                                      aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                              >  2:3401/1‑93 (MQ=255)
                                                      aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                              >  1:2681836/1‑93 (MQ=255)
                                                      aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                              <  1:3765583/93‑1 (MQ=255)
                                                      aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                              >  1:5800499/1‑93 (MQ=255)
                                                       aCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc                             >  2:2842010/1‑93 (MQ=255)
                                                        ccGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg                            <  2:477486/93‑1 (MQ=255)
                                                         cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg                           >  2:5040711/1‑93 (MQ=255)
                                                         cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg                           >  1:1679641/1‑93 (MQ=255)
                                                         cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg                           <  1:1986766/93‑1 (MQ=255)
                                                          gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAACCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt                          <  1:386499/93‑1 (MQ=255)
                                                           aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt                         >  2:4463652/1‑93 (MQ=255)
                                                            tGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc                        >  1:4649880/1‑93 (MQ=255)
                                                              cGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCgg                      <  2:56513/93‑1 (MQ=255)
                                                               gAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa                     >  2:4018558/1‑93 (MQ=255)
                                                               gAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa                     >  2:593526/1‑93 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    <  1:6119340/93‑1 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    >  2:3581542/1‑93 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    >  1:5716343/1‑93 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    >  2:823753/1‑93 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    <  1:3642599/93‑1 (MQ=255)
                                                                 aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAg                   >  1:2347428/1‑93 (MQ=255)
                                                                  aaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGt                  <  1:1519996/93‑1 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 <  2:3454881/93‑1 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 <  2:3051746/93‑1 (MQ=255)
                                                                   aGGACAACAAGGGGCCGAATGCGAGGCGCGCGTATGACACGCAAGCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 <  1:940900/93‑1 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                >  1:3973104/1‑93 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                <  1:5383874/93‑1 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCACCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                >  2:2474385/1‑93 (MQ=255)
                                                                      acaacaAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgggg              >  2:5445209/1‑93 (MQ=255)
                                                                       caacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAACCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGt             <  2:1831688/93‑1 (MQ=255)
                                                                        aacaaGGGGGCGAATGCGAGGCGCGCGTATGACGCGCAAACTCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTGGGAAGTGGGGtt            >  2:1908586/1‑93 (MQ=255)
                                                                        aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGtt            >  1:3530407/1‑93 (MQ=255)
                                                                         acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc           <  1:4677267/93‑1 (MQ=255)
                                                                         acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc           >  2:389363/1‑93 (MQ=255)
                                                                         acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc           >  2:3182394/1‑93 (MQ=255)
                                                                         acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc           >  1:3594045/1‑93 (MQ=255)
                                                                          caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTcc          >  1:1513854/1‑93 (MQ=255)
                                                                          caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTcc          <  2:5755829/93‑1 (MQ=255)
                                                                           aaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTccc         >  1:1042938/1‑93 (MQ=255)
                                                                            aGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCg        >  1:3904960/1‑93 (MQ=255)
                                                                                  cGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGc  <  1:6190694/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGC  >  NC_000913/3352167‑3352341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  NC_000913/3352161‑3352343
                                                                                             |                                                                                         
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATG                                                                                           >  SRR4302534.5539848/1‑94 (MQ=60)
     GAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGG                                                                                      <  SRR4302534.3784145/94‑1 (MQ=60)
      AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCTCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGC                                                                                     >  SRR4302534.581579/1‑94 (MQ=60)
      AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGC                                                                                     >  SRR4302534.2113024/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCG                                                                                  >  SRR4302534.484460/1‑94 (MQ=60)
          GCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGC                                                                                 <  SRR4302534.1672314/94‑1 (MQ=60)
           CCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCG                                                                                >  SRR4302534.4718379/1‑94 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGT                                                                               >  SRR4302534.554324/1‑94 (MQ=60)
             TGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTA                                                                              >  SRR4302534.2412570/1‑94 (MQ=60)
             TGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTA                                                                              <  SRR4302534.5455526/94‑1 (MQ=60)
             TGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGAGGCGAATGCGAGGCGCGCGTA                                                                              <  SRR4302534.6388629/94‑1 (MQ=60)
              GCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAT                                                                             <  SRR4302534.4976851/94‑1 (MQ=60)
               CTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGTGGCGAATGCGAGGCGCGCGTATG                                                                            >  SRR4302534.5371512/1‑94 (MQ=60)
               CTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATG                                                                            >  SRR4302534.4146959/1‑94 (MQ=60)
                TCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTGTTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGA                                                                           >  SRR4302534.1760199/1‑94 (MQ=60)
                TCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGA                                                                           >  SRR4302534.1281534/1‑94 (MQ=60)
                TCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGA                                                                           <  SRR4302534.2803615/94‑1 (MQ=60)
                 CATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGAC                                                                          >  SRR4302534.2891833/1‑94 (MQ=60)
                 CATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGAC                                                                          >  SRR4302534.1442807/1‑94 (MQ=60)
                    CATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACG                                                                       >  SRR4302534.3827789/1‑94 (MQ=60)
                    CATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACG                                                                       <  SRR4302534.2310336/94‑1 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGC                                                                      >  SRR4302534.4053600/1‑94 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGC                                                                      >  SRR4302534.4867434/1‑94 (MQ=60)
                       ATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAA                                                                    <  SRR4302534.3125378/94‑1 (MQ=60)
                         TTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAC                                                                  >  SRR4302534.4006587/1‑94 (MQ=60)
                         TTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAC                                                                  <  SRR4302534.3662098/94‑1 (MQ=60)
                          TATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGAACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACC                                                                 <  SRR4302534.5079359/94‑1 (MQ=60)
                           ATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCC                                                                >  SRR4302534.6567459/1‑94 (MQ=60)
                            TGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCC                                                               >  SRR4302534.1324358/1‑94 (MQ=60)
                            TGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCC                                                               <  SRR4302534.1556065/94‑1 (MQ=60)
                            TGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCC                                                               >  SRR4302534.2452918/1‑94 (MQ=60)
                              CAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGT                                                             >  SRR4302534.3794589/1‑94 (MQ=60)
                               AGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTC                                                            <  SRR4302534.1342824/94‑1 (MQ=60)
                               AGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTC                                                            <  SRR4302534.5675041/94‑1 (MQ=60)
                                 TAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGC                                                          <  SRR4302534.3327616/94‑1 (MQ=60)
                                  AATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCC                                                         >  SRR4302534.4952937/1‑94 (MQ=60)
                                     TGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACG                                                      <  SRR4302534.4605680/94‑1 (MQ=60)
                                       AGATCCCCTCTTCACCGTATTATCCGATGCGAAAAGGACAACAAGGGGCCGAATGCGAGGCGCGCGTATCACACGCAAACCCCGTCGCCCCGCT                                                    <  SRR4302534.922482/94‑1 (MQ=60)
                                        GATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTC                                                   >  SRR4302534.5650404/1‑94 (MQ=60)
                                        GATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTC                                                   >  SRR4302534.6376408/1‑94 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCT                                                  <  SRR4302534.6123195/94‑1 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCT                                                  >  SRR4302534.4856709/1‑94 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCT                                                  <  SRR4302534.3208288/94‑1 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 >  SRR4302534.961518/1‑94 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 >  SRR4302534.551998/1‑94 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 <  SRR4302534.767681/94‑1 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                >  SRR4302534.586214/1‑94 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                >  SRR4302534.4867784/1‑94 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                <  SRR4302534.6407543/94‑1 (MQ=60)
                                            CCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTC                                               >  SRR4302534.2286153/1‑94 (MQ=60)
                                            CCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTC                                               >  SRR4302534.2511231/1‑94 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGGAAAGGACAACCAAGGGGCGGATGCGAGACGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              >  SRR4302534.2627435/1‑94 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              <  SRR4302534.3719282/94‑1 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              >  SRR4302534.5677565/1‑94 (MQ=60)
                                               TCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGT                                            <  SRR4302534.949692/94‑1 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           <  SRR4302534.6580287/94‑1 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           >  SRR4302534.892229/1‑94 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           <  SRR4302534.1890189/94‑1 (MQ=60)
                                                 TTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGC                                          >  SRR4302534.5937668/1‑94 (MQ=60)
                                                 TTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTCTGC                                          >  SRR4302534.1266310/1‑94 (MQ=60)
                                                  TCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCC                                         >  SRR4302534.1989356/1‑94 (MQ=60)
                                                  TCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCC                                         >  SRR4302534.1541560/1‑94 (MQ=60)
                                                    ACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCG                                       <  SRR4302534.2767680/94‑1 (MQ=60)
                                                     CCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGT                                      >  SRR4302534.4900500/1‑94 (MQ=60)
                                                       GTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGC                                    <  SRR4302534.3472529/94‑1 (MQ=60)
                                                        TATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCG                                   >  SRR4302534.360387/1‑94 (MQ=60)
                                                        TATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCG                                   <  SRR4302534.5191150/94‑1 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                <  SRR4302534.4488771/94‑1 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                >  SRR4302534.4709526/1‑94 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                >  SRR4302534.6503143/1‑94 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                >  SRR4302534.5957911/1‑94 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                >  SRR4302534.2752726/1‑94 (MQ=60)
                                                            AACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGC                               >  SRR4302534.5319921/1‑94 (MQ=60)
                                                            AACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGC                               <  SRR4302534.3866677/94‑1 (MQ=60)
                                                             ACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCG                              >  SRR4302534.869699/1‑94 (MQ=60)
                                                             ACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCG                              >  SRR4302534.4742467/1‑94 (MQ=60)
                                                              CCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGG                             >  SRR4302534.1723344/1‑94 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            <  SRR4302534.2038789/94‑1 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302534.6616935/1‑94 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302534.5804167/1‑94 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302534.2819450/1‑94 (MQ=60)
                                                                GATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAACCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           <  SRR4302534.396390/94‑1 (MQ=60)
                                                                GATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           >  SRR4302534.5532006/1‑94 (MQ=60)
                                                                GATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           >  SRR4302534.395619/1‑94 (MQ=60)
                                                                GATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           >  SRR4302534.6152561/1‑94 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          <  SRR4302534.3100736/94‑1 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          <  SRR4302534.6569817/94‑1 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          >  SRR4302534.3514927/1‑94 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          >  SRR4302534.4776109/1‑94 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          <  SRR4302534.1750359/94‑1 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          >  SRR4302534.6286906/1‑94 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          >  SRR4302534.5585842/1‑94 (MQ=60)
                                                                   GCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGG                        >  SRR4302534.2021834/1‑94 (MQ=60)
                                                                     GAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAA                      >  SRR4302534.5871223/1‑94 (MQ=60)
                                                                     GAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAA                      >  SRR4302534.6009985/1‑94 (MQ=60)
                                                                     GAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAA                      <  SRR4302534.4540587/94‑1 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     <  SRR4302534.6285793/94‑1 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     >  SRR4302534.4077181/1‑94 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     <  SRR4302534.3740495/94‑1 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     >  SRR4302534.3297278/1‑94 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     >  SRR4302534.2409718/1‑94 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   <  SRR4302534.1559912/94‑1 (MQ=60)
                                                                         AGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGG                  >  SRR4302534.4079448/1‑94 (MQ=60)
                                                                         AGGACAACAAGGGGCCGAATGCGAGGCGCGCGTATGACACGCAAGCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGG                  <  SRR4302534.964826/94‑1 (MQ=60)
                                                                          GGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGG                 <  SRR4302534.5528894/94‑1 (MQ=60)
                                                                          GGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGG                 <  SRR4302534.4777643/94‑1 (MQ=60)
                                                                          GGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGCTTCGGAAGCGGG                 >  SRR4302534.1179638/1‑94 (MQ=60)
                                                                           GACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGG                <  SRR4302534.670171/94‑1 (MQ=60)
                                                                           GACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGG                >  SRR4302534.3415254/1‑94 (MQ=60)
                                                                             CAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              >  SRR4302534.1459236/1‑94 (MQ=60)
                                                                             CAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              >  SRR4302534.2677524/1‑94 (MQ=60)
                                                                             CAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              >  SRR4302534.3625362/1‑94 (MQ=60)
                                                                              AACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             >  SRR4302534.3690707/1‑94 (MQ=60)
                                                                              AACAAGCGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             >  SRR4302534.4183355/1‑94 (MQ=60)
                                                                               ACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCC            >  SRR4302534.1553644/1‑94 (MQ=60)
                                                                               ACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCC            <  SRR4302534.4804241/94‑1 (MQ=60)
                                                                                CAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCC           >  SRR4302534.4127039/1‑94 (MQ=60)
                                                                                CAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCC           >  SRR4302534.1069870/1‑94 (MQ=60)
                                                                                 AAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          >  SRR4302534.4009681/1‑94 (MQ=60)
                                                                                 AAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          >  SRR4302534.4707755/1‑94 (MQ=60)
                                                                                 AAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          <  SRR4302534.5970775/94‑1 (MQ=60)
                                                                                 AAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          >  SRR4302534.5015370/1‑94 (MQ=60)
                                                                                  AGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGC         <  SRR4302534.925120/94‑1 (MQ=60)
                                                                                   GGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCA        <  SRR4302534.1908974/94‑1 (MQ=60)
                                                                                    GGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAG       >  SRR4302534.10126/1‑94 (MQ=60)
                                                                                     GGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGA      >  SRR4302534.239101/1‑94 (MQ=60)
                                                                                     GGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGA      >  SRR4302534.1354297/1‑94 (MQ=60)
                                                                                      GGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAG     <  SRR4302534.5938018/94‑1 (MQ=60)
                                                                                        CGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCC   <  SRR4302534.6359155/94‑1 (MQ=60)
                                                                                        CGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCC   >  SRR4302534.4257109/1‑94 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302534.3397913/94‑1 (MQ=60)
                                                                                             |                                                                                         
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  NC_000913/3352161‑3352343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑