Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,362,543	T→C	V701A (GTA→GCA)	yhcD →	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,362,543	0	T	C	99.6%	813.6 / ‑4.6	230	V701A (GTA→GCA)	yhcD	putative outer membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (120/109); total (120/110)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

Only 100 of 240 total aligned reads displayed.

CATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAACTGCCCAGAGGCGTTG  >  NC_000913/3362453‑3362632

cATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGcagcag                                                                                         >  2:3191740/1‑93 (MQ=255)

  tCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAggg                                                                                       <  1:3725317/93‑1 (MQ=255)

  tCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAggg                                                                                       >  2:3617202/1‑93 (MQ=255)

   cAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGa                                                                                      >  1:5760694/1‑93 (MQ=255)

   cAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGa                                                                                      <  1:3896444/93‑1 (MQ=255)

    aGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGaa                                                                                     <  2:5224352/93‑1 (MQ=255)

      gAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAg                                                                                   <  2:6348081/93‑1 (MQ=255)

       aGTTGAGATCGATCCCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGt                                                                                  <  2:5913684/93‑1 (MQ=255)

       aGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGt                                                                                  <  1:5312933/93‑1 (MQ=255)

        gTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTc                                                                                 >  1:3992840/1‑93 (MQ=255)

           gagaTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGa                                                                              <  2:2241240/93‑1 (MQ=255)

            agaTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGAt                                                                             >  1:5188270/1‑93 (MQ=255)

             gaTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGAtt                                                                            >  1:6031695/1‑93 (MQ=255)

             gaTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGAtt                                                                            >  2:831569/1‑93 (MQ=255)

             gaTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGAtt                                                                            <  2:930228/93‑1 (MQ=255)

             gaTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGAtt                                                                            >  1:3450310/1‑93 (MQ=255)

              aTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTc                                                                           <  1:283124/93‑1 (MQ=255)

              aTCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTc                                                                           >  2:2914710/1‑93 (MQ=255)

               tCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCa                                                                          <  1:673080/93‑1 (MQ=255)

               tCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCa                                                                          >  2:5118198/1‑93 (MQ=255)

                cGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAg                                                                         >  2:3650802/1‑93 (MQ=255)

                 gATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGt                                                                        >  1:4041467/1‑93 (MQ=255)

                 gATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGt                                                                        <  2:5777839/93‑1 (MQ=255)

                 gATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCAGATTCAGt                                                                        >  2:3927954/1‑93 (MQ=255)

                  aTACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTg                                                                       >  1:5447421/1‑93 (MQ=255)

                  aTACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTg                                                                       >  1:3099679/1‑93 (MQ=255)

                   tACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGc                                                                      >  2:5386480/1‑93 (MQ=255)

                    aCCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCg                                                                     >  2:4653991/1‑93 (MQ=255)

                     ccGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGa                                                                    >  1:1755689/1‑93 (MQ=255)

                      cGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGAt                                                                   <  1:1692228/93‑1 (MQ=255)

                      cGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGAt                                                                   <  2:2412760/93‑1 (MQ=255)

                       gCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATa                                                                  <  2:792736/93‑1 (MQ=255)

                        ccAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATaa                                                                 <  1:759473/93‑1 (MQ=255)

                        ccAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATaa                                                                 <  1:2214922/93‑1 (MQ=255)

                         cAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAac                                                                >  1:5912021/1‑93 (MQ=255)

                         cAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAac                                                                <  1:1095304/93‑1 (MQ=255)

                           gCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAacac                                                              <  2:2749329/93‑1 (MQ=255)

                           gCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAacac                                                              >  2:3015526/1‑93 (MQ=255)

                              tGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAAc                                                           <  1:5420574/93‑1 (MQ=255)

                              tGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAAc                                                           <  2:4991847/93‑1 (MQ=255)

                              tGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAAc                                                           >  1:3554239/1‑93 (MQ=255)

                                ccAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGc                                                         <  1:5733336/93‑1 (MQ=255)

                                ccAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGACGGATTCAGTGCGATAACACAACGc                                                         >  2:6241476/1‑93 (MQ=255)

                                 cAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGcc                                                        <  2:1423806/93‑1 (MQ=255)

                                 cAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGcc                                                        >  1:3872244/1‑93 (MQ=255)

                                  aaaaaaTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCg                                                       <  1:4389229/93‑1 (MQ=255)

                                   aaaaaTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGt                                                      <  1:6375515/93‑1 (MQ=255)

                                    aaaaTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTg                                                     >  2:3988929/1‑93 (MQ=255)

                                      aaTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCa                                                   <  1:3600847/93‑1 (MQ=255)

                                      aaTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCa                                                   >  2:1760927/1‑93 (MQ=255)

                                       aTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCAt                                                  <  1:5962103/93‑1 (MQ=255)

                                       aTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCAt                                                  <  1:4305573/93‑1 (MQ=255)

                                       aTGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCAt                                                  >  1:3266843/1‑93 (MQ=255)

                                        tGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCAtt                                                 <  2:4513491/93‑1 (MQ=255)

                                            gATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTc                                             <  1:3516498/93‑1 (MQ=255)

                                              tatCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCaa                                           >  2:2298263/1‑93 (MQ=255)

                                               atCGGTAACGGCACAAAAATGATCGAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAat                                          <  1:1000242/93‑1 (MQ=255)

                                                tCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAata                                         <  1:5592579/93‑1 (MQ=255)

                                                 cGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAatat                                        <  2:3036191/93‑1 (MQ=255)

                                                   gTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATca                                      >  2:1325198/1‑93 (MQ=255)

                                                   gTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATca                                      >  2:4456532/1‑93 (MQ=255)

                                                    tAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATcac                                     <  1:1451687/93‑1 (MQ=255)

                                                     aaCGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATcaca                                    >  2:4833257/1‑93 (MQ=255)

                                                      aCGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCacac                                   >  2:799298/1‑93 (MQ=255)

                                                       cGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACt                                  <  1:2599736/93‑1 (MQ=255)

                                                        ggCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACtt                                 <  1:1887819/93‑1 (MQ=255)

                                                        ggCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACtt                                 <  1:897520/93‑1 (MQ=255)

                                                         gCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACttt                                <  2:5603858/93‑1 (MQ=255)

                                                            caAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCg                             >  2:1903004/1‑93 (MQ=255)

                                                              aaaaTGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCggc                           >  1:3309823/1‑91 (MQ=255)

                                                              aaaaTGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCggc                           >  2:6210267/1‑91 (MQ=255)

                                                              aaaaTGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCggc                           <  2:1978827/93‑3 (MQ=255)

                                                              aaaaTGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCggc                           <  1:1171135/93‑3 (MQ=255)

                                                                aaTGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCcggcgg                         <  2:3069077/93‑1 (MQ=255)

                                                                aaTGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCcggcgg                         >  2:3412785/1‑93 (MQ=255)

                                                                 aTGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCggcggc                        >  2:244787/1‑93 (MQ=255)

                                                                   gATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCaa                      >  1:1573261/1‑93 (MQ=255)

                                                                       aaaCAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCaaaaaa                  >  1:4611845/1‑93 (MQ=255)

                                                                        aaCAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAAc                 >  2:1087685/1‑93 (MQ=255)

                                                                         aCAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACt                <  1:4124438/93‑1 (MQ=255)

                                                                         aCAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACt                <  1:2055427/93‑1 (MQ=255)

                                                                          cAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTg               <  1:2660189/93‑1 (MQ=255)

                                                                           aaGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGc              >  1:2467171/1‑93 (MQ=255)

                                                                            aGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGcc             >  2:1214544/1‑93 (MQ=255)

                                                                            aGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGcc             >  2:1270203/1‑93 (MQ=255)

                                                                             ggCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGccc            >  1:2579528/1‑93 (MQ=255)

                                                                              gCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCa           <  2:4202988/93‑1 (MQ=255)

                                                                               ccGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCag          >  2:1553367/1‑93 (MQ=255)

                                                                               ccGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCag          >  1:4831278/1‑93 (MQ=255)

                                                                                 gtggtgCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCagag        <  1:520999/93‑1 (MQ=255)

                                                                                 gtggtgCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCagag        >  1:5677050/1‑93 (MQ=255)

                                                                                 gtggtgCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCagag        <  1:6176786/93‑1 (MQ=255)

                                                                                  tggtgCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAgg       >  1:2131514/1‑93 (MQ=255)

                                                                                  tggcgCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAgg       >  1:3260441/1‑93 (MQ=255)

                                                                                   ggtgCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGc      <  2:237313/93‑1 (MQ=255)

                                                                                     tgCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGt    >  2:5961488/1‑93 (MQ=255)

                                                                                      gcagcaGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGtt   >  2:4950023/1‑93 (MQ=255)

                                                                                      gcagcaGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGtt   <  1:6125166/93‑1 (MQ=255)

                                                                                       cagcagGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTg  >  1:5672107/1‑93 (MQ=255)

                                                                                       cagcagGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTg  <  1:4366577/93‑1 (MQ=255)

CATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAACTGCCCAGAGGCGTTG  >  NC_000913/3362453‑3362632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAACTGCCCAGAGGCGTTGCGAT  >  NC_000913/3362450‑3362636

GAACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGC                                                                                               >  SRR4302534.3704388/1‑94 (MQ=60)

GAACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGC                                                                                               >  SRR4302534.6577895/1‑94 (MQ=60)

GAACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGC                                                                                               >  SRR4302534.1776145/1‑94 (MQ=60)

 AACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGCTCAAACAAGGCCGTGGTGCAGCA                                                                                              >  SRR4302534.2057717/1‑94 (MQ=60)

 AACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCA                                                                                              <  SRR4302534.6008993/94‑1 (MQ=60)

 AACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCA                                                                                              >  SRR4302534.6563487/1‑94 (MQ=60)

   CATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGG                                                                                            >  SRR4302534.2879246/1‑94 (MQ=60)

     TCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGA                                                                                          <  SRR4302534.3825453/94‑1 (MQ=60)

     TCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGA                                                                                          >  SRR4302534.5916970/1‑94 (MQ=60)

      CAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAA                                                                                         <  SRR4302534.4001011/94‑1 (MQ=60)

      CAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATCTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAA                                                                                         >  SRR4302534.1801102/1‑94 (MQ=60)

       AGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAA                                                                                        <  SRR4302534.1617822/94‑1 (MQ=60)

       AGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAA                                                                                        <  SRR4302534.3236344/94‑1 (MQ=60)

        GGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTTACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAG                                                                                       >  SRR4302534.5883730/1‑94 (MQ=60)

        GGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAG                                                                                       <  SRR4302534.2625370/94‑1 (MQ=60)

        GGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAG                                                                                       <  SRR4302534.6063696/94‑1 (MQ=60)

          AGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGCAAGTG                                                                                     >  SRR4302534.6045080/1‑94 (MQ=60)

          AGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTC                                                                                     <  SRR4302534.3637104/94‑1 (MQ=60)

          AGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTC                                                                                     <  SRR4302534.5456272/94‑1 (MQ=60)

          AGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTC                                                                                     >  SRR4302534.4099722/1‑94 (MQ=60)

          AGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTC                                                                                     >  SRR4302534.76533/1‑94 (MQ=60)

              GAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGAT                                                                                 <  SRR4302534.4129460/94‑1 (MQ=60)

              GAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGAT                                                                                 >  SRR4302534.5328115/1‑94 (MQ=60)

               AGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATT                                                                                >  SRR4302534.3543058/1‑94 (MQ=60)

               AGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATT                                                                                <  SRR4302534.6408478/94‑1 (MQ=60)

               AGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATT                                                                                >  SRR4302534.4499210/1‑94 (MQ=60)

               AGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATT                                                                                <  SRR4302534.4750660/94‑1 (MQ=60)

               AGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATT                                                                                >  SRR4302534.6195599/1‑94 (MQ=60)

               AGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATT                                                                                >  SRR4302534.5329791/1‑94 (MQ=60)

                GATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTC                                                                               >  SRR4302534.3652290/1‑94 (MQ=60)

                 ATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCA                                                                              <  SRR4302534.290435/94‑1 (MQ=60)

                 ATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCA                                                                              <  SRR4302534.3990754/94‑1 (MQ=60)

                 ATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCA                                                                              <  SRR4302534.1132163/94‑1 (MQ=60)

                  TCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAG                                                                             <  SRR4302534.689497/94‑1 (MQ=60)

                   CGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGT                                                                            <  SRR4302534.5527784/94‑1 (MQ=60)

                   CGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGT                                                                            >  SRR4302534.4149828/1‑94 (MQ=60)

                    GATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTG                                                                           >  SRR4302534.5594189/1‑94 (MQ=60)

                    GATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTG                                                                           >  SRR4302534.3181218/1‑94 (MQ=60)

                    GATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTG                                                                           >  SRR4302534.1912582/1‑94 (MQ=60)

                      TACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCG                                                                         <  SRR4302534.196981/94‑1 (MQ=60)

                       ACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGA                                                                        >  SRR4302534.1801284/1‑94 (MQ=60)

                        CCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGAT                                                                       <  SRR4302534.3349638/94‑1 (MQ=60)

                         CGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATA                                                                      <  SRR4302534.1736212/94‑1 (MQ=60)

                           CCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAAC                                                                    >  SRR4302534.6072567/1‑94 (MQ=60)

                           CCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAAC                                                                    <  SRR4302534.778317/94‑1 (MQ=60)

                           CCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAAC                                                                    >  SRR4302534.1699293/1‑94 (MQ=60)

                           CCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAAC                                                                    <  SRR4302534.2273584/94‑1 (MQ=60)

                            CAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACA                                                                   <  SRR4302534.1123794/94‑1 (MQ=60)

                               CTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAA                                                                <  SRR4302534.5786283/94‑1 (MQ=60)

                                TTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAAC                                                               >  SRR4302534.3649824/1‑94 (MQ=60)

                                 TGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACG                                                              <  SRR4302534.5566438/94‑1 (MQ=60)

                                 TGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACG                                                              <  SRR4302534.2124492/94‑1 (MQ=60)

                                   CCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCC                                                            >  SRR4302534.3976221/1‑94 (MQ=60)

                                   CCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCC                                                            <  SRR4302534.5888699/94‑1 (MQ=60)

                                    CAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCG                                                           <  SRR4302534.965940/94‑1 (MQ=60)

                                    CAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCG                                                           >  SRR4302534.2661978/1‑94 (MQ=60)

                                    CAAAAAAGGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCG                                                           >  SRR4302534.2665668/1‑94 (MQ=60)

                                     AAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGT                                                          >  SRR4302534.2850224/1‑94 (MQ=60)

                                     AAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGT                                                          <  SRR4302534.4507584/94‑1 (MQ=60)

                                     AAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGT                                                          <  SRR4302534.566213/94‑1 (MQ=60)

                                      AAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTG                                                         <  SRR4302534.6547708/94‑1 (MQ=60)

                                         AATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCAT                                                      <  SRR4302534.3697684/94‑1 (MQ=60)

                                         AATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCAT                                                      >  SRR4302534.2256729/1‑94 (MQ=60)

                                         AATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCAT                                                      >  SRR4302534.3353850/1‑94 (MQ=60)

                                          ATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATT                                                     <  SRR4302534.3556928/94‑1 (MQ=60)

                                          ATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATT                                                     <  SRR4302534.4422280/94‑1 (MQ=60)

                                          ATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATT                                                     <  SRR4302534.6123982/94‑1 (MQ=60)

                                          ATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATT                                                     <  SRR4302534.1581817/94‑1 (MQ=60)

                                           TGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTA                                                    <  SRR4302534.4308895/94‑1 (MQ=60)

                                             TCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACT                                                  >  SRR4302534.2499352/1‑94 (MQ=60)

                                              CGATATCTGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTC                                                 >  SRR4302534.5502526/1‑94 (MQ=60)

                                              CGATATCGGTAGTGGCACAAAAATGATCAAACAAGGCCGTGGTCGCGCAGGGAAAGTCGGTGTCAGTGCGATAACACAACGGCGTGCACTACGC                                                 <  SRR4302534.2574410/94‑1 (MQ=40)

                                              CGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTC                                                 >  SRR4302534.447074/1‑94 (MQ=60)

                                               GATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCA                                                <  SRR4302534.3611083/94‑1 (MQ=60)

                                                 TATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAAT                                              <  SRR4302534.6571702/94‑1 (MQ=60)

                                                  ATCGGTAACGGCACAAAAATGATCGAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATA                                             <  SRR4302534.1025875/94‑1 (MQ=60)

                                                  ATCGGTAACGGCACAAAAATGATCATACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATA                                             <  SRR4302534.2689612/94‑1 (MQ=60)

                                                  ATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATA                                             <  SRR4302534.4106948/94‑1 (MQ=60)

                                                   TCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATAT                                            <  SRR4302534.5743776/94‑1 (MQ=60)

                                                       TAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACA                                        <  SRR4302534.3352628/94‑1 (MQ=60)

                                                       TAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACA                                        >  SRR4302534.4002139/1‑94 (MQ=60)

                                                       TAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACA                                        <  SRR4302534.1489874/94‑1 (MQ=60)

                                                        AACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACAC                                       >  SRR4302534.5861184/1‑94 (MQ=60)

                                                        AACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACAC                                       >  SRR4302534.3672072/1‑94 (MQ=60)

                                                         ACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACT                                      >  SRR4302534.3528860/1‑94 (MQ=60)

                                                         ACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACT                                      >  SRR4302534.3880215/1‑94 (MQ=60)

                                                          CGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTT                                     <  SRR4302534.2668528/94‑1 (MQ=60)

                                                          CGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTT                                     <  SRR4302534.1990538/94‑1 (MQ=60)

                                                           GGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTT                                    <  SRR4302534.1937042/94‑1 (MQ=60)

                                                           GGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTT                                    <  SRR4302534.920248/94‑1 (MQ=60)

                                                           GGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTT                                    <  SRR4302534.305876/94‑1 (MQ=60)

                                                           GGCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTT                                    <  SRR4302534.63605/94‑1 (MQ=60)

                                                            GCACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTC                                   <  SRR4302534.112112/94‑1 (MQ=60)

                                                             CACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCC                                  <  SRR4302534.5079095/94‑1 (MQ=60)

                                                              ACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACGCTTTCCG                                 <  SRR4302534.6300051/94‑1 (MQ=60)

                                                              ACAAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCG                                 <  SRR4302534.1340561/94‑1 (MQ=60)

                                                                AAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGC                               >  SRR4302534.4601574/1‑94 (MQ=60)

                                                                AAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGC                               >  SRR4302534.393300/1‑94 (MQ=60)

                                                                AAAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGC                               >  SRR4302534.3398215/1‑94 (MQ=60)

                                                                 AAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCG                              <  SRR4302534.1201918/94‑1 (MQ=60)

                                                                 AAAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCG                              <  SRR4302534.5586666/94‑1 (MQ=60)

                                                                  AAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGG                             >  SRR4302534.5915308/1‑94 (MQ=60)

                                                                  AAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGG                             <  SRR4302534.5724385/94‑1 (MQ=60)

                                                                  AAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGG                             >  SRR4302534.6201267/1‑94 (MQ=60)

                                                                  AAATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGG                             <  SRR4302534.3749496/94‑1 (MQ=60)

                                                                   AATGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGC                            >  SRR4302534.6285381/1‑94 (MQ=60)

                                                                     TGATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAA                          >  SRR4302534.1614452/1‑94 (MQ=60)

                                                                       ATCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAA                        >  SRR4302534.5417853/1‑94 (MQ=60)

                                                                        TCAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAA                       >  SRR4302534.6082588/1‑94 (MQ=60)

                                                                         CAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAA                      <  SRR4302534.829551/94‑1 (MQ=60)

                                                                         CAAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAA                      >  SRR4302534.4737010/1‑94 (MQ=60)

                                                                          AAACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAAC                     >  SRR4302534.4702943/1‑94 (MQ=60)

                                                                            ACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTG                   <  SRR4302534.2109124/94‑1 (MQ=60)

                                                                            ACAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTG                   <  SRR4302534.4235456/94‑1 (MQ=60)

                                                                             CAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGC                  >  SRR4302534.2532754/1‑94 (MQ=60)

                                                                             CAAGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGC                  <  SRR4302534.2730523/94‑1 (MQ=60)

                                                                               AGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCC                >  SRR4302534.4238204/1‑94 (MQ=60)

                                                                               AGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCC                <  SRR4302534.3369237/94‑1 (MQ=60)

                                                                               AGGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCC                >  SRR4302534.2647846/1‑94 (MQ=60)

                                                                                GGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCA               <  SRR4302534.5826144/94‑1 (MQ=60)

                                                                                GGCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCA               >  SRR4302534.1202634/1‑94 (MQ=60)

                                                                                 GCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAG              >  SRR4302534.4222896/1‑94 (MQ=60)

                                                                                 GCCGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAG              >  SRR4302534.4962351/1‑94 (MQ=60)

                                                                                   CGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAG            >  SRR4302534.5830747/1‑94 (MQ=60)

                                                                                   CGTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAG            >  SRR4302534.5000267/1‑94 (MQ=60)

                                                                                    GTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGG           <  SRR4302534.6344878/94‑1 (MQ=60)

                                                                                    GTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGG           <  SRR4302534.533951/94‑1 (MQ=60)

                                                                                    GTGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGG           >  SRR4302534.2187670/1‑94 (MQ=60)

                                                                                    GTGGCGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGG           >  SRR4302534.3347261/1‑94 (MQ=60)

                                                                                     TGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGC          <  SRR4302534.6154277/94‑1 (MQ=60)

                                                                                     TGGTGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGC          <  SRR4302534.831032/94‑1 (MQ=60)

                                                                                        TGCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTT       <  SRR4302534.3430315/94‑1 (MQ=60)

                                                                                         GCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTG      <  SRR4302534.2202333/94‑1 (MQ=60)

                                                                                         GCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTG      <  SRR4302534.6291773/94‑1 (MQ=60)

                                                                                         GCAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTG      >  SRR4302534.5825681/1‑94 (MQ=60)

                                                                                          CAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTGC     >  SRR4302534.5684082/1‑94 (MQ=60)

                                                                                          CAGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTGC     <  SRR4302534.4484281/94‑1 (MQ=60)

                                                                                           AGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTGCG    <  SRR4302534.4963529/94‑1 (MQ=60)

                                                                                           AGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTGCG    <  SRR4302534.2852668/94‑1 (MQ=60)

                                                                                           AGCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTGCG    >  SRR4302534.5977758/1‑94 (MQ=60)

                                                                                            GCAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTGCGA   <  SRR4302534.5607347/94‑1 (MQ=60)

                                                                                             CAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGGCGGCAAAAAACTGCCCAGAGGCGTTGCGAT  <  SRR4302534.4675508/94‑1 (MQ=60)

GAACATCAGGAGTTGAGATCGATACCGCCAGCTTGCCAAAAAATGTCGATATCGGTAACGGCACAAAAATGATCAAACAAGGCCGTGGTGCAGTAGGGAAAGTCGGATTCAGTGCGATAACACAACGCCGTGCATTACTCAATATCACACTTTCCGACGGCAAAAAACTGCCCAGAGGCGTTGCGAT  >  NC_000913/3362450‑3362636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`