Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,371,573 A→G G181G (GGT→GGC)  b3226 ← putative FADA‑type transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,371,5730AG99.6% 876.8 / ‑5.0 257G181G (GGT→GGC) b3226putative FADA‑type transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (117/139);  total (118/139)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 260 total aligned reads displayed.

ATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTACCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCA  >  NC_000913/3371483‑3371661
                                                                                          |                                                                                        
aTGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGcc                                                                                        <  2:795728/93‑1 (MQ=255)
aTGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGcc                                                                                        >  2:491166/1‑93 (MQ=255)
 tGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCg                                                                                       >  1:1219290/1‑93 (MQ=255)
 tGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCg                                                                                       <  2:3589998/93‑1 (MQ=255)
  gTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCgg                                                                                      <  1:3179664/93‑1 (MQ=255)
   ttCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCggg                                                                                     <  1:1605771/93‑1 (MQ=255)
    tCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCgggg                                                                                    <  2:6151726/93‑1 (MQ=255)
     cGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGa                                                                                   <  1:3671689/93‑1 (MQ=255)
      gTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAt                                                                                  >  1:6171171/1‑93 (MQ=255)
      gTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAt                                                                                  <  1:4995975/93‑1 (MQ=255)
      gTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAt                                                                                  <  1:3079823/93‑1 (MQ=255)
       tGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAtc                                                                                 <  1:5690053/93‑1 (MQ=255)
        gCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAtct                                                                                >  1:1180909/1‑93 (MQ=255)
         cAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAtctc                                                                               <  1:3010387/93‑1 (MQ=255)
             gCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGcc                                                                           <  1:4360097/93‑1 (MQ=255)
              ccTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGTATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCa                                                                          <  2:467189/93‑1 (MQ=255)
              ccTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCa                                                                          <  2:19449/93‑1 (MQ=255)
               cTGATGAGTAACCGTTGGGCGTGCGGCAATAGGCCAGTCGAGCAGGGCAACGTGAATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAg                                                                         <  2:440359/93‑1 (MQ=255)
                 gATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAAGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCa                                                                       <  2:6227558/93‑1 (MQ=255)
                  aTCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCTGCCAGCAc                                                                      <  1:6299362/93‑1 (MQ=255)
                  aTCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAc                                                                      >  1:293927/1‑93 (MQ=255)
                  aTCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAc                                                                      <  2:4270443/93‑1 (MQ=255)
                  aTCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAc                                                                      <  2:5181061/93‑1 (MQ=255)
                    cGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAcgc                                                                    <  1:4441007/93‑1 (MQ=255)
                     ggTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAcgcg                                                                   >  2:2402026/1‑93 (MQ=255)
                     ggTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAcgcg                                                                   <  1:6381911/93‑1 (MQ=255)
                       tAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGt                                                                 >  2:3869774/1‑93 (MQ=255)
                        aaCCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTg                                                                >  2:6307274/1‑93 (MQ=255)
                        aaCCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTg                                                                >  1:493517/1‑93 (MQ=255)
                         aCCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCCACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAAGCGGTgg                                                               >  1:3292005/1‑93 (MQ=255)
                         aCCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTgg                                                               <  2:1539478/93‑1 (MQ=255)
                           cGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGaa                                                             <  2:2852365/93‑1 (MQ=255)
                            gTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGaaa                                                            >  1:2896927/1‑93 (MQ=255)
                            gTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGaaa                                                            >  2:4970600/1‑93 (MQ=255)
                                ggCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCaa                                                        <  1:1465614/93‑1 (MQ=255)
                                 gCGTGCGGCAATAAGCGAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAAc                                                       <  1:2555385/93‑1 (MQ=255)
                                       ggCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGa                                                 <  1:3373443/93‑1 (MQ=255)
                                       ggCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGa                                                 <  2:5979720/93‑1 (MQ=255)
                                        gCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGaa                                                >  1:4187700/1‑93 (MQ=255)
                                        gCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGaa                                                >  1:4130933/1‑93 (MQ=255)
                                         cAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAAc                                               <  1:2337533/93‑1 (MQ=255)
                                           aTAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGGAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACga                                             >  2:4504067/1‑93 (MQ=255)
                                           aTAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACga                                             <  2:2639439/93‑1 (MQ=255)
                                            tAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaa                                            <  2:2413991/93‑1 (MQ=255)
                                             aaGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaat                                           <  2:4244841/93‑1 (MQ=255)
                                              aGCCAGTCGAGCAGGGCAACGTGGATCGCCATGCAGATTGGGTTGCCGCGGATCTCCGCCAGCACGCGGTGGCAATCAACGTCTGAACgaatg                                          >  1:3935201/1‑93 (MQ=255)
                                              aGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatg                                          >  2:5624626/1‑93 (MQ=255)
                                              aGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatg                                          >  1:3585458/1‑93 (MQ=255)
                                               gCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatga                                         >  2:3496639/1‑93 (MQ=255)
                                               gCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatga                                         <  2:70009/93‑1 (MQ=255)
                                               gCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGATTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatga                                         >  1:5243991/1‑93 (MQ=255)
                                                ccAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatgaa                                        >  2:528367/1‑93 (MQ=255)
                                                ccAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatgaa                                        <  2:1971038/93‑1 (MQ=255)
                                                ccAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatgaa                                        >  1:5016155/1‑93 (MQ=255)
                                                 cAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatgaat                                       <  1:3468005/93‑1 (MQ=255)
                                                 cAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACgaatgaat                                       <  1:1633169/93‑1 (MQ=255)
                                                 cAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTAAACgaatgaat                                       <  1:403869/93‑1 (MQ=255)
                                                  aGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGaatgaatg                                      >  2:3636024/1‑93 (MQ=255)
                                                  aGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGaatgaatg                                      <  1:4696778/93‑1 (MQ=255)
                                                   gTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGc                                     >  1:1712331/1‑93 (MQ=255)
                                                     cGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCcg                                   >  2:3681619/1‑93 (MQ=255)
                                                     cGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCcg                                   <  2:1663419/93‑1 (MQ=255)
                                                      gAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCcgc                                  >  1:773236/1‑93 (MQ=255)
                                                       aGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCcgcg                                 >  2:1795616/1‑93 (MQ=255)
                                                       aGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCcgcg                                 <  2:5134018/93‑1 (MQ=255)
                                                        gCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCgt                                >  1:2152497/1‑93 (MQ=255)
                                                           gggCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCgttgt                             >  2:2784860/1‑93 (MQ=255)
                                                           gggCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCgttgt                             <  2:2225313/93‑1 (MQ=255)
                                                            ggCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCgttgtt                            >  1:5996641/1‑93 (MQ=255)
                                                             gCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTa                           <  1:2753292/93‑1 (MQ=255)
                                                               aaCGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATc                         >  2:3113069/1‑93 (MQ=255)
                                                                aCGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATcc                        <  2:3975864/93‑1 (MQ=255)
                                                                aCGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATcc                        <  2:2428674/93‑1 (MQ=255)
                                                                aCGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATcc                        >  2:1182432/1‑93 (MQ=255)
                                                                 cGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCa                       <  2:6448853/93‑1 (MQ=255)
                                                                  gTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAg                      <  1:3788863/93‑1 (MQ=255)
                                                                   tGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGc                     <  2:2318970/93‑1 (MQ=255)
                                                                    ggATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCg                    <  2:5316819/93‑1 (MQ=255)
                                                                      aTCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCgac                  <  2:1632410/93‑1 (MQ=255)
                                                                        cGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCgactg                <  1:1353894/93‑1 (MQ=255)
                                                                         gCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCgactga               >  2:3043211/1‑93 (MQ=255)
                                                                          ccATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCgactgac              >  2:1309318/1‑93 (MQ=255)
                                                                          ccATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCgactgac              <  1:912036/93‑1 (MQ=255)
                                                                           cATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCgactgact             >  2:3212580/1‑93 (MQ=255)
                                                                           cATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCgactgact             <  1:5765357/93‑1 (MQ=255)
                                                                            aTGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGactgactg            >  2:5760559/1‑93 (MQ=255)
                                                                            aTGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGactgactg            >  1:4463087/1‑93 (MQ=255)
                                                                              gAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGtt          <  1:5949589/93‑1 (MQ=255)
                                                                               aaGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTg         <  2:3745435/93‑1 (MQ=255)
                                                                                aGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGa        <  1:5071083/93‑1 (MQ=255)
                                                                                aGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGa        <  1:1726848/93‑1 (MQ=255)
                                                                                 gATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAt       <  1:3198825/93‑1 (MQ=255)
                                                                                 gATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAt       <  1:4660434/93‑1 (MQ=255)
                                                                                 gATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAt       >  2:555667/1‑93 (MQ=255)
                                                                                  aTTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGCATGAATGCCCCGTTGTTATCCAGCGACTGACTGTTGGtt      >  1:1528037/1‑93 (MQ=255)
                                                                                  aTTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAtt      <  2:4887925/93‑1 (MQ=255)
                                                                                  aTTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAtt      <  2:2537811/93‑1 (MQ=255)
                                                                                   ttGGGTTGCCGAGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAttt     <  2:5532534/93‑1 (MQ=255)
                                                                                    tGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTc    <  1:5397211/93‑1 (MQ=255)
                                                                                      ggTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCa  <  2:2084231/93‑1 (MQ=255)
                                                                                          |                                                                                        
ATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTACCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCA  >  NC_000913/3371483‑3371661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTACCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCT  >  NC_000913/3371480‑3371666
                                                                                             |                                                                                             
GTTATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTG                                                                                               >  SRR4302534.6178899/1‑94 (MQ=60)
 TTATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGC                                                                                              <  SRR4302534.1255177/94‑1 (MQ=60)
 TTATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGC                                                                                              <  SRR4302534.6014569/94‑1 (MQ=60)
 TTATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGC                                                                                              <  SRR4302534.4212901/94‑1 (MQ=60)
   ATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCG                                                                                            >  SRR4302534.1251390/1‑94 (MQ=60)
   ATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCG                                                                                            >  SRR4302534.1549325/1‑94 (MQ=60)
    TGTTCGTGCCGTGCCTGATCCGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGG                                                                                           >  SRR4302534.6490770/1‑94 (MQ=60)
    TGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGG                                                                                           <  SRR4302534.5126306/94‑1 (MQ=60)
     GTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGG                                                                                          >  SRR4302534.1403734/1‑94 (MQ=60)
     GTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGG                                                                                          <  SRR4302534.3263652/94‑1 (MQ=60)
     GTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGG                                                                                          >  SRR4302534.3425709/1‑94 (MQ=60)
      TTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGG                                                                                         <  SRR4302534.1647705/94‑1 (MQ=60)
       TCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGA                                                                                        <  SRR4302534.4906606/94‑1 (MQ=60)
       TCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGA                                                                                        >  SRR4302534.3849380/1‑94 (MQ=60)
        CGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAT                                                                                       >  SRR4302534.6339043/1‑94 (MQ=60)
        CGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAT                                                                                       >  SRR4302534.6339044/1‑94 (MQ=60)
        CGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAT                                                                                       <  SRR4302534.3323675/94‑1 (MQ=60)
        CGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAT                                                                                       <  SRR4302534.3770400/94‑1 (MQ=60)
        CGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGAT                                                                                       >  SRR4302534.6107714/1‑94 (MQ=60)
        CGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTACCGGGGAT                                                                                       >  SRR4302534.6575679/1‑94 (MQ=60)
         GTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATC                                                                                      <  SRR4302534.3160741/94‑1 (MQ=60)
         GTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATC                                                                                      >  SRR4302534.2994845/1‑94 (MQ=60)
         GTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATC                                                                                      <  SRR4302534.5131091/94‑1 (MQ=60)
          TGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCT                                                                                     <  SRR4302534.5844159/94‑1 (MQ=60)
          TGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCT                                                                                     >  SRR4302534.1211948/1‑94 (MQ=60)
            CAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCC                                                                                   <  SRR4302534.4736284/94‑1 (MQ=60)
            CAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCC                                                                                   >  SRR4302534.129198/1‑94 (MQ=60)
            CAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCC                                                                                   >  SRR4302534.1286040/1‑94 (MQ=60)
            CAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCC                                                                                   <  SRR4302534.3089158/94‑1 (MQ=60)
             AGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCG                                                                                  >  SRR4302534.6117462/1‑94 (MQ=60)
                GCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCA                                                                               <  SRR4302534.4477715/94‑1 (MQ=60)
                 CCTGATCGGTAATCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAG                                                                              >  SRR4302534.3460663/1‑94 (MQ=60)
                   TGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCA                                                                            >  SRR4302534.5137153/1‑94 (MQ=60)
                   TGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCA                                                                            <  SRR4302534.4863086/94‑1 (MQ=60)
                    GATCTGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAC                                                                           <  SRR4302534.1664873/94‑1 (MQ=60)
                    GATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAC                                                                           >  SRR4302534.301527/1‑94 (MQ=60)
                    GATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAC                                                                           >  SRR4302534.1766097/1‑94 (MQ=60)
                     ATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCTGCCAGCACG                                                                          <  SRR4302534.6469726/94‑1 (MQ=60)
                     ATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACG                                                                          <  SRR4302534.3559433/94‑1 (MQ=60)
                     ATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACG                                                                          <  SRR4302534.4005491/94‑1 (MQ=60)
                      TCGGTAACGGTGGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGC                                                                         <  SRR4302534.270075/94‑1 (MQ=60)
                       CGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCG                                                                        <  SRR4302534.4560972/94‑1 (MQ=60)
                        GGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGG                                                                       <  SRR4302534.6554298/94‑1 (MQ=60)
                          TAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTG                                                                     <  SRR4302534.3008024/94‑1 (MQ=60)
                          TAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTG                                                                     >  SRR4302534.505829/1‑94 (MQ=60)
                          TAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTG                                                                     <  SRR4302534.3075964/94‑1 (MQ=60)
                           AACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCCACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCAAGCGGTGG                                                                    >  SRR4302534.3379782/1‑94 (MQ=60)
                           AACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTACGCCAGCACGCGGTGG                                                                    >  SRR4302534.3261218/1‑94 (MQ=60)
                              CGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAA                                                                 >  SRR4302534.2972968/1‑94 (MQ=60)
                               GTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAAT                                                                >  SRR4302534.311767/1‑94 (MQ=60)
                               GTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAAT                                                                <  SRR4302534.6304721/94‑1 (MQ=60)
                                TTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATC                                                               <  SRR4302534.5500879/94‑1 (MQ=60)
                                  GGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAA                                                             >  SRR4302534.4006321/1‑94 (MQ=60)
                                   GGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAAC                                                            <  SRR4302534.1504161/94‑1 (MQ=60)
                                    GCGTGCGGCAATAAGCGAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACG                                                           <  SRR4302534.2623119/94‑1 (MQ=60)
                                    GCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACG                                                           <  SRR4302534.6108376/94‑1 (MQ=60)
                                    GCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACG                                                           <  SRR4302534.5838155/94‑1 (MQ=60)
                                      GTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTC                                                         <  SRR4302534.2643687/94‑1 (MQ=60)
                                          GGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAA                                                     <  SRR4302534.3463841/94‑1 (MQ=60)
                                          GGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAA                                                     >  SRR4302534.4242272/1‑94 (MQ=60)
                                          GGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAA                                                     <  SRR4302534.4566553/94‑1 (MQ=60)
                                          GGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAA                                                     >  SRR4302534.4300860/1‑94 (MQ=60)
                                            CAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACG                                                   <  SRR4302534.2399566/94‑1 (MQ=60)
                                             AATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGA                                                  >  SRR4302534.4555722/1‑94 (MQ=60)
                                               TAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAAT                                                >  SRR4302534.1606436/1‑94 (MQ=60)
                                                aagccactctaggttgtccaggaGGATCGCCATGAAGGTTGGGTTCCCGGGGATCTCCGCCAGCACGCGGTCGAAAGCAACGTCTGAACGAATG                                               <  SRR4302534.2961973/71‑1 (MQ=60)
                                                AAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGCAGATTGGGTTGCCGCGGATCTCCGCCAGCACGCGGTGGCAATCAACGTCTGAACGAATG                                               >  SRR4302534.4040609/1‑94 (MQ=60)
                                                AAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATG                                               >  SRR4302534.4040610/1‑94 (MQ=60)
                                                AAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATG                                               >  SRR4302534.3681899/1‑94 (MQ=60)
                                                 AGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGATTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGA                                              >  SRR4302534.5385626/1‑94 (MQ=60)
                                                  GCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAA                                             >  SRR4302534.5151702/1‑94 (MQ=60)
                                                   CCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAAT                                            <  SRR4302534.3753616/94‑1 (MQ=60)
                                                   CCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAAT                                            >  SRR4302534.1674808/1‑94 (MQ=60)
                                                   CCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAAT                                            >  SRR4302534.4453464/1‑94 (MQ=60)
                                                    CAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATG                                           <  SRR4302534.5866180/94‑1 (MQ=60)
                                                    CAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATG                                           <  SRR4302534.3561213/94‑1 (MQ=60)
                                                    CAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATG                                           <  SRR4302534.1675742/94‑1 (MQ=60)
                                                    CAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATG                                           <  SRR4302534.1949373/94‑1 (MQ=60)
                                                    CAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTAAACGAATGAATG                                           <  SRR4302534.414190/94‑1 (MQ=60)
                                                     AGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGC                                          <  SRR4302534.4824260/94‑1 (MQ=60)
                                                     AGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGC                                          >  SRR4302534.1756811/1‑94 (MQ=60)
                                                       TCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCG                                        <  SRR4302534.2304654/94‑1 (MQ=60)
                                                        CGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGC                                       >  SRR4302534.792496/1‑94 (MQ=60)
                                                          AGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGT                                     >  SRR4302534.2209314/1‑94 (MQ=60)
                                                          AGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGT                                     >  SRR4302534.2430131/1‑94 (MQ=60)
                                                           GCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTT                                    <  SRR4302534.5164094/94‑1 (MQ=60)
                                                           GCAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTT                                    <  SRR4302534.4621131/94‑1 (MQ=60)
                                                            CAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTG                                   >  SRR4302534.3601988/1‑94 (MQ=60)
                                                            CAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTG                                   >  SRR4302534.5222614/1‑94 (MQ=60)
                                                            CAGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACATCTGAACGAATGAATGCCGCGTTG                                   >  SRR4302534.922319/1‑94 (MQ=60)
                                                             AGGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGT                                  <  SRR4302534.3267169/94‑1 (MQ=60)
                                                              GGGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTT                                 >  SRR4302534.6159551/1‑94 (MQ=60)
                                                               GGCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTA                                <  SRR4302534.6376649/94‑1 (MQ=60)
                                                                GCAACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTAT                               <  SRR4302534.2825819/94‑1 (MQ=60)
                                                                  AACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCC                             >  SRR4302534.6168801/1‑94 (MQ=60)
                                                                  AACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCC                             >  SRR4302534.4500198/1‑94 (MQ=60)
                                                                   ACGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCA                            >  SRR4302534.542766/1‑94 (MQ=60)
                                                                    CGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAG                           >  SRR4302534.734487/1‑94 (MQ=60)
                                                                    CGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAG                           <  SRR4302534.999126/94‑1 (MQ=60)
                                                                    CGTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGGTATCGAG                           >  SRR4302534.1685323/1‑94 (MQ=60)
                                                                     GTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGC                          <  SRR4302534.123363/94‑1 (MQ=60)
                                                                     GTGGATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGC                          <  SRR4302534.3890581/94‑1 (MQ=60)
                                                                         ATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACT                      <  SRR4302534.4376951/94‑1 (MQ=60)
                                                                         ATCGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACT                      <  SRR4302534.3074076/94‑1 (MQ=60)
                                                                           CGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGA                    <  SRR4302534.1389578/94‑1 (MQ=60)
                                                                           CGCCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGA                    >  SRR4302534.1612966/1‑94 (MQ=60)
                                                                             CCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACT                  <  SRR4302534.935235/94‑1 (MQ=60)
                                                                             CCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACT                  <  SRR4302534.1743217/94‑1 (MQ=60)
                                                                             CCATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACT                  >  SRR4302534.5159256/1‑94 (MQ=60)
                                                                              CATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTG                 >  SRR4302534.4583762/1‑94 (MQ=60)
                                                                              CATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTG                 >  SRR4302534.2298600/1‑94 (MQ=60)
                                                                              CATGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTG                 <  SRR4302534.5921766/94‑1 (MQ=60)
                                                                                TGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTT               >  SRR4302534.5655198/1‑94 (MQ=60)
                                                                                TGAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTT               >  SRR4302534.2899453/1‑94 (MQ=60)
                                                                                 GAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTG              >  SRR4302534.3605565/1‑94 (MQ=60)
                                                                                 GAAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTG              <  SRR4302534.6111134/94‑1 (MQ=60)
                                                                                  AAGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGA             >  SRR4302534.3867185/1‑94 (MQ=60)
                                                                                   AGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAT            <  SRR4302534.2127919/94‑1 (MQ=60)
                                                                                   AGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAT            <  SRR4302534.5207990/94‑1 (MQ=60)
                                                                                   AGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAT            <  SRR4302534.1771688/94‑1 (MQ=60)
                                                                                   AGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAT            >  SRR4302534.330077/1‑94 (MQ=60)
                                                                                   AGATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGAT            <  SRR4302534.4189289/94‑1 (MQ=60)
                                                                                    GATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGCATGAATGCCCCGTTGTTATCCAGCGACTGACTGTTGGTT           >  SRR4302534.1568145/1‑94 (MQ=60)
                                                                                    GATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATT           <  SRR4302534.3283634/94‑1 (MQ=60)
                                                                                    GATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATT           <  SRR4302534.4786965/94‑1 (MQ=60)
                                                                                     ATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTT          <  SRR4302534.3210164/94‑1 (MQ=60)
                                                                                     ATTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTT          <  SRR4302534.5120682/94‑1 (MQ=60)
                                                                                      TTGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTC         <  SRR4302534.1507060/94‑1 (MQ=60)
                                                                                       TGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCC        >  SRR4302534.4283788/1‑94 (MQ=60)
                                                                                       TGGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCC        <  SRR4302534.5542528/94‑1 (MQ=60)
                                                                                        GGGTTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCA       <  SRR4302534.4809699/94‑1 (MQ=60)
                                                                                           TTGCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTG    <  SRR4302534.4190250/94‑1 (MQ=60)
                                                                                             GCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCT  <  SRR4302534.3896202/94‑1 (MQ=60)
                                                                                             GCCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCT  <  SRR4302534.1421314/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTTATGTTCGTGCAGTGCCTGATCGGTAACCGTTGGGCGTGCGGCAATAAGCCAGTCGAGCAGGGCAACGTGGATCGCCATGAAGATTGGGTTACCGGGGATCTCCGCCAGCACGCGGTGGAAATCAACGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCT  >  NC_000913/3371480‑3371666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑