Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,393,106	Δ1 bp	intergenic (‑3443/+857)	tldD ← / ← cafA	TldD/CafA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,393,103	0	T	.	99.5%	1016.4 / ‑2.6	217	intergenic (‑3440/+860)	tldD/cafA	TldD/CafA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (104/112); minor base A (1/0); total (105/112)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT  >  NC_000913/3393011‑3393195

 aGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtt                                                                                             <  1:1205004/93‑1 (MQ=255)

  gCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGttt                                                                                            >  2:2306096/1‑93 (MQ=255)

    ccGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttca                                                                                          <  2:5500552/93‑3 (MQ=255)

     cGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcag                                                                                         <  2:1732512/93‑4 (MQ=255)

      ggATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                        >  2:1423926/1‑89 (MQ=255)

      ggATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                        >  1:4324676/1‑89 (MQ=255)

       gATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGcc                                                                                      >  2:1174903/1‑93 (MQ=255)

       gATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGcc                                                                                      >  2:2816780/1‑93 (MQ=255)

        aTTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑CTTCAGCCa                                                                                     >  1:5770495/1‑93 (MQ=255)

         ttttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAg                                                                                    >  2:5599503/1‑93 (MQ=255)

         ttttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAg                                                                                    >  1:2489761/1‑93 (MQ=255)

           ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                                  <  1:3416105/93‑1 (MQ=255)

            tCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACg                                                                                 >  1:4123535/1‑93 (MQ=255)

             cACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGt                                                                                <  1:6077598/93‑1 (MQ=255)

              aCGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTc                                                                               <  1:5414593/93‑1 (MQ=255)

               cgcgTAATATGCGTGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCa                                                                              >  2:739630/1‑93 (MQ=255)

               cgcgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCa                                                                              >  2:3779074/1‑93 (MQ=255)

                gcgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAc                                                                             >  1:2293472/1‑93 (MQ=255)

                gcgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAc                                                                             >  1:6414082/1‑93 (MQ=255)

                 cgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAcc                                                                            <  1:1040220/93‑1 (MQ=255)

                  gTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccg                                                                           >  1:5141598/1‑93 (MQ=255)

                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                          >  1:6150919/1‑93 (MQ=255)

                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                          <  2:2578715/93‑1 (MQ=255)

                    aaTATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgcc                                                                         >  1:19064/1‑93 (MQ=255)

                     atatGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCCCCCAACCAGCTGGCAGCGCCCTG‑GTTCAGCCAGCCGTCAccgccg                                                                        >  1:3968684/1‑93 (MQ=255)

                      tatTCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCgg                                                                       >  1:805217/1‑93 (MQ=255)

                      tatGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCgg                                                                       >  1:3697843/1‑93 (MQ=255)

                       atGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGt                                                                      <  2:4806545/93‑1 (MQ=255)

                        tGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGtt                                                                     <  1:5923521/93‑1 (MQ=255)

                        tGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGtt                                                                     >  1:1689031/1‑93 (MQ=255)

                          cgcgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTAc                                                                   <  1:2550842/93‑1 (MQ=255)

                             ggTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATc                                                                <  2:4547223/93‑1 (MQ=255)

                              gTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCg                                                               >  2:1356461/1‑93 (MQ=255)

                               tCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGc                                                              >  1:2322777/1‑93 (MQ=255)

                                cAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGcc                                                             <  2:3751356/93‑1 (MQ=255)

                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCtt                                                           >  1:4722338/1‑93 (MQ=255)

                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCtt                                                           >  1:3751125/1‑93 (MQ=255)

                                   aTTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCttt                                                          <  2:5551004/93‑1 (MQ=255)

                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTc                                                       >  2:2636025/1‑93 (MQ=255)

                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGGTACATCGCCTTTGtg                                                       >  2:1242/1‑92 (MQ=255)

                                       tCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCg                                                      >  2:4660894/1‑93 (MQ=255)

                                       tCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCg                                                      <  2:5004103/93‑1 (MQ=255)

                                        ccACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGa                                                     <  2:5157395/93‑1 (MQ=255)

                                        ccACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGa                                                     >  2:598681/1‑93 (MQ=255)

                                         cACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAt                                                    >  2:180826/1‑93 (MQ=255)

                                         cACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAt                                                    >  2:2408824/1‑93 (MQ=255)

                                          aCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATc                                                   >  1:6463403/1‑93 (MQ=255)

                                          aCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATc                                                   <  2:3024840/93‑1 (MQ=255)

                                           ccGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCg                                                  >  1:5469200/1‑93 (MQ=255)

                                           ccGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCg                                                  >  2:805360/1‑93 (MQ=255)

                                            cGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGt                                                 <  2:224394/93‑1 (MQ=255)

                                            cGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGt                                                 <  2:4737441/93‑1 (MQ=255)

                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                                >  1:4238578/1‑93 (MQ=255)

                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                                >  1:1409731/1‑93 (MQ=255)

                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                                >  1:1312704/1‑93 (MQ=255)

                                              aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCa                                               >  1:786042/1‑93 (MQ=255)

                                              aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCa                                               >  1:5645238/1‑93 (MQ=255)

                                              aCAGCCTAGTCGTTTGCTTCTCGCCCATCCAGCTGCCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCa                                               <  1:3224549/93‑1 (MQ=255)

                                                aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                             <  2:1537865/93‑1 (MQ=255)

                                                aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                             <  1:4205407/93‑1 (MQ=255)

                                                aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                             <  1:4455485/93‑1 (MQ=255)

                                                aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                             <  1:3485034/93‑1 (MQ=255)

                                                aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                             <  1:1933446/93‑1 (MQ=255)

                                                 gCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                            <  1:5584093/93‑1 (MQ=255)

                                                 gCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCT‑ATTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                            >  2:3434416/1‑93 (MQ=255)

                                                     aTGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGcc                                        >  1:4778087/1‑93 (MQ=255)

                                                      tGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                       <  2:538570/93‑1 (MQ=255)

                                                      tGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                       >  1:3240737/1‑93 (MQ=255)

                                                      tGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                       <  2:668098/93‑1 (MQ=255)

                                                         gTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCa                                    >  1:1528680/1‑93 (MQ=255)

                                                         gTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCCGCTACATCGCCTTTGTCGATCGTCATCCAGCCTTCa                                    >  1:3597090/1‑93 (MQ=255)

                                                          tttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCaa                                   >  1:1607335/1‑93 (MQ=255)

                                                           ttGCTTCTCGCGCAACCAGCTGGCACCGCCCTG‑TTGCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAg                                  <  2:712029/93‑1 (MQ=255)

                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                                >  1:2609229/1‑93 (MQ=255)

                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                                <  1:1137122/93‑1 (MQ=255)

                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                                <  1:5377361/93‑1 (MQ=255)

                                                                tctcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGgaga                             <  2:4020138/93‑1 (MQ=255)

                                                                tctcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATAGCCTTTGTCGATCGTCATCCAGCCTTCAAGGgaga                             >  2:566028/1‑93 (MQ=255)

                                                                   cGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACt                          <  2:5856687/93‑1 (MQ=255)

                                                                   cGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACt                          <  2:209662/93‑1 (MQ=255)

                                                                   cGCCCAACCAGCTGGCACCGCCCTG‑TTGCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACt                          <  2:2801576/93‑1 (MQ=255)

                                                                     cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgt                        <  2:1613258/93‑1 (MQ=255)

                                                                     cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgt                        <  1:5437228/93‑1 (MQ=255)

                                                                      ccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgtg                       >  1:1293633/1‑93 (MQ=255)

                                                                       cAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGc                      <  2:2957503/93‑1 (MQ=255)

                                                                        caCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGcc                     <  2:6464773/92‑1 (MQ=255)

                                                                        aaCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGcc                     <  2:3518346/93‑1 (MQ=255)

                                                                          ccAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGt                   <  1:981872/93‑1 (MQ=255)

                                                                           cAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGtt                  <  2:3866018/93‑1 (MQ=255)

                                                                            aGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGttt                 <  1:3158345/93‑1 (MQ=255)

                                                                             gCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTc                <  2:5027116/93‑1 (MQ=255)

                                                                             gCTGCCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTc                <  1:3864738/93‑1 (MQ=255)

                                                                              cTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTcc               >  1:5950338/1‑93 (MQ=255)

                                                                              cTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTcc               <  2:4395924/93‑1 (MQ=255)

                                                                               tGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCa              <  2:6069843/93‑1 (MQ=255)

                                                                               cgccACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTGCATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCa              <  1:4474409/90‑1 (MQ=255)

                                                                                ggCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAg             <  2:1943174/93‑1 (MQ=255)

                                                                                    ccGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAAGGAGAACTGTGCCGTTTCCAGCGCa         >  1:5266530/1‑93 (MQ=255)

                                                                                      gCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAat       <  2:6096285/93‑1 (MQ=255)

                                                                                       cccTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAata      >  1:3506634/1‑93 (MQ=255)

CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT  >  NC_000913/3393011‑3393195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG  >  NC_000913/3393015‑3393199

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAG                                                                                            >  SRR4302534.4584178/1‑94 (MQ=60)

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAG                                                                                            <  SRR4302534.1516791/94‑1 (MQ=60)

 CGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGC                                                                                           >  SRR4302534.4441691/1‑94 (MQ=60)

   GATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑CTTCAGCCA                                                                                         >  SRR4302534.5927035/1‑94 (MQ=60)

    ATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAG                                                                                        >  SRR4302534.2555896/1‑94 (MQ=60)

    ATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAG                                                                                        >  SRR4302534.4722391/1‑94 (MQ=60)

      TTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCTTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                      <  SRR4302534.3565457/94‑1 (MQ=60)

      TTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                      <  SRR4302534.3316718/94‑1 (MQ=60)

      TTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                      >  SRR4302534.5102714/1‑94 (MQ=60)

       TTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACG                                                                                     <  SRR4302534.3507881/94‑1 (MQ=60)

       TTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACG                                                                                     <  SRR4302534.558964/94‑1 (MQ=60)

       TTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACG                                                                                     >  SRR4302534.4234529/1‑94 (MQ=60)

        TCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTTCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGT                                                                                    >  SRR4302534.3846260/1‑94 (MQ=60)

         CACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTC                                                                                   <  SRR4302534.6242872/94‑1 (MQ=60)

          CCGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCA                                                                                  <  SRR4302534.1938375/94‑1 (MQ=60)

          ACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCA                                                                                  <  SRR4302534.3777101/94‑1 (MQ=60)

          ACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCA                                                                                  <  SRR4302534.5560243/94‑1 (MQ=60)

           CGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAC                                                                                 >  SRR4302534.2558037/1‑94 (MQ=60)

           CGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAC                                                                                 >  SRR4302534.2354348/1‑94 (MQ=60)

           CGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAC                                                                                 >  SRR4302534.6587142/1‑94 (MQ=60)

            GCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACT                                                                                >  SRR4302534.4124476/1‑94 (MQ=60)

             CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCCGACGTCACCG                                                                               >  SRR4302534.3293248/1‑94 (MQ=60)

             CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCG                                                                               >  SRR4302534.1967954/1‑94 (MQ=60)

             CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCG                                                                               >  SRR4302534.5280355/1‑94 (MQ=60)

             CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCG                                                                               <  SRR4302534.1067071/94‑1 (MQ=60)

              GTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGC                                                                              >  SRR4302534.6318260/1‑94 (MQ=60)

               TAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCC                                                                             >  SRR4302534.19568/1‑94 (MQ=60)

               TAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTAAGCAAGACGTCACGGCC                                                                             >  SRR4302534.1622266/1‑94 (MQ=60)

               TAATATGCGACGTAAGATTATCCACCGACAGCGTATGCGCTTGCCACTCAACCACCCAGCTGCCCCCGCCCTG‑TTTCAGCCAGACGTCACCGCC                                                                             <  SRR4302534.5037840/94‑1 (MQ=21)

                AATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCCCCCAACCAGCTGGCAGCGCCCTG‑GTTCAGCCAGCCGTCACCGCCG                                                                            >  SRR4302534.4074922/1‑94 (MQ=60)

                 ATATTCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGG                                                                           >  SRR4302534.825449/1‑94 (MQ=60)

                 ATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGG                                                                           >  SRR4302534.3797257/1‑94 (MQ=60)

                  TATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGT                                                                          >  SRR4302534.1321231/1‑94 (MQ=60)

                   ATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTT                                                                         <  SRR4302534.5535257/94‑1 (MQ=60)

                   ATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTT                                                                         >  SRR4302534.1732942/1‑94 (MQ=60)

                   ATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTT                                                                         >  SRR4302534.1628868/1‑94 (MQ=60)

                    TGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTA                                                                        <  SRR4302534.6084358/94‑1 (MQ=60)

                    TGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTA                                                                        >  SRR4302534.1393102/1‑94 (MQ=60)

                      CGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACA                                                                      >  SRR4302534.6430898/1‑94 (MQ=60)

                      CGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACA                                                                      <  SRR4302534.2618471/94‑1 (MQ=60)

                       GCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACAT                                                                     >  SRR4302534.5512208/1‑94 (MQ=60)

                         GGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCG                                                                   <  SRR4302534.3825201/94‑1 (MQ=60)

                          GTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGC                                                                  >  SRR4302534.2384424/1‑94 (MQ=60)

                             AGATTATCCACCGTCAGCGTATGCGTTTGCTACTCGCCCAACCAGCTGGCGCCGCCCTG‑TTTCAGCCAGACGTCGCCGCCGGTTACATCGCCTT                                                               <  SRR4302534.1748346/94‑1 (MQ=60)

                             AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCCACCAGCTTGCACCGCCCCG‑TTTCAGCCAGACGTCCCCGCCGGgtacctcggcct                                                               >  SRR4302534.2004001/1‑82 (MQ=60)

                             AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTT                                                               >  SRR4302534.1188696/1‑94 (MQ=60)

                             AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTT                                                               >  SRR4302534.3851864/1‑94 (MQ=60)

                             AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTT                                                               >  SRR4302534.4850596/1‑94 (MQ=60)

                              GATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTT                                                              <  SRR4302534.3587031/94‑1 (MQ=60)

                              GATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTAAATCGCCTTT                                                              <  SRR4302534.6466791/94‑1 (MQ=60)

                                TTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGT                                                            <  SRR4302534.2371250/94‑1 (MQ=60)

                                TTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGT                                                            <  SRR4302534.3008074/94‑1 (MQ=60)

                                  ATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCG                                                          >  SRR4302534.871964/1‑94 (MQ=60)

                                    CCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAT                                                        >  SRR4302534.3680813/1‑94 (MQ=60)

                                    CCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAT                                                        >  SRR4302534.1270110/1‑94 (MQ=60)

                                     CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                       >  SRR4302534.6637487/1‑94 (MQ=60)

                                     CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                       <  SRR4302534.432696/94‑1 (MQ=60)

                                     CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                       >  SRR4302534.3393500/1‑94 (MQ=60)

                                     CACCGACAGCGGATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGGTACATCGCCTTTGTCGATC                                                       >  SRR4302534.5841792/1‑94 (MQ=60)

                                      ACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCG                                                      >  SRR4302534.5616622/1‑94 (MQ=60)

                                       CCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGT                                                     <  SRR4302534.1443429/94‑1 (MQ=60)

                                        CGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTC                                                    >  SRR4302534.4352856/1‑94 (MQ=60)

                                        CGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTC                                                    >  SRR4302534.1446816/1‑94 (MQ=60)

                                        CGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTC                                                    >  SRR4302534.4058577/1‑94 (MQ=60)

                                        CGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTC                                                    >  SRR4302534.1347321/1‑94 (MQ=60)

                                         GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                   >  SRR4302534.4128640/1‑94 (MQ=60)

                                         GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                   >  SRR4302534.5798044/1‑94 (MQ=60)

                                         GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                   >  SRR4302534.805708/1‑94 (MQ=60)

                                         GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                   <  SRR4302534.3862255/94‑1 (MQ=60)

                                          acagcctagtCGTTTGCTTCTCGCCCATCCAGCTGCCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCAT                                                  <  SRR4302534.3310200/84‑1 (MQ=60)

                                            AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                <  SRR4302534.1983998/94‑1 (MQ=60)

                                            AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                <  SRR4302534.4575923/94‑1 (MQ=60)

                                            AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                >  SRR4302534.591360/1‑94 (MQ=60)

                                            AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                <  SRR4302534.4542254/94‑1 (MQ=60)

                                            AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                <  SRR4302534.3578711/94‑1 (MQ=60)

                                            AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                <  SRR4302534.4318930/94‑1 (MQ=60)

                                             GCGTCTGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCCGTTACCTCGCCCTTGCCGATCGTCATCCA                                               >  SRR4302534.3729888/1‑94 (MQ=60)

                                             GCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCA                                               <  SRR4302534.3182220/94‑1 (MQ=60)

                                             GCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCA                                               <  SRR4302534.5734982/94‑1 (MQ=60)

                                               GTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGC                                             <  SRR4302534.2584473/94‑1 (MQ=60)

                                               GTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGC                                             <  SRR4302534.3315280/94‑1 (MQ=60)

                                                TATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCC                                            >  SRR4302534.2332443/1‑94 (MQ=60)

                                                TATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCC                                            <  SRR4302534.3547595/94‑1 (MQ=60)

                                                TATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCC                                            >  SRR4302534.4907838/1‑94 (MQ=60)

                                                 ATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCT                                           >  SRR4302534.4317894/1‑94 (MQ=60)

                                                 ATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCT                                           >  SRR4302534.3326967/1‑94 (MQ=60)

                                                 ATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCT                                           >  SRR4302534.1673232/1‑94 (MQ=60)

                                                  TGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTT                                          <  SRR4302534.1135637/94‑1 (MQ=60)

                                                  TGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTT                                          <  SRR4302534.5101977/94‑1 (MQ=60)

                                                    CGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCA                                        <  SRR4302534.5628306/94‑1 (MQ=60)

                                                    CGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCA                                        >  SRR4302534.1568798/1‑94 (MQ=60)

                                                    CGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCCGCTACATCGCCTTTGTCGATCGTCATCCAGCCTTCA                                        >  SRR4302534.3693828/1‑94 (MQ=60)

                                                     GTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAA                                       >  SRR4302534.1649313/1‑94 (MQ=60)

                                                     GTTTGCTTCTCGCCCAACCAGCTGGCAACGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAACGTCATCCAGACTTCCA                                       >  SRR4302534.2846867/1‑94 (MQ=60)

                                                      TTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAG                                      >  SRR4302534.5751088/1‑94 (MQ=60)

                                                        TGCTTCTCGCCCACCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGG                                    <  SRR4302534.4870766/94‑1 (MQ=60)

                                                        TGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGG                                    >  SRR4302534.825428/1‑94 (MQ=60)

                                                        TGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGG                                    >  SRR4302534.2678270/1‑94 (MQ=60)

                                                         GCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGA                                   <  SRR4302534.5522246/94‑1 (MQ=60)

                                                         GCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGA                                   <  SRR4302534.1166855/94‑1 (MQ=60)

                                                          CTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAG                                  >  SRR4302534.5595170/1‑94 (MQ=60)

                                                          CTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAG                                  <  SRR4302534.4547391/94‑1 (MQ=60)

                                                           TTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGA                                 <  SRR4302534.5927538/94‑1 (MQ=60)

                                                              TCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACT                              <  SRR4302534.4732319/94‑1 (MQ=60)

                                                                cgcccagcaagctgccgccgcgctgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTG                             <  SRR4302534.4498000/69‑1 (MQ=60)

                                                                 CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTG                           >  SRR4302534.1327699/1‑94 (MQ=60)

                                                                 CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTG                           <  SRR4302534.5583661/94‑1 (MQ=60)

                                                                  CCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGC                          <  SRR4302534.1327832/94‑1 (MQ=60)

                                                                  CCAACCAGCTGGCACCGCCATG‑TTTCAGCCAGAAGTCACAGCAGGTTCCATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGAGAGAACTGTGC                          >  SRR4302534.1024928/1‑94 (MQ=60)

                                                                    AACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCG                        >  SRR4302534.662992/1‑94 (MQ=60)

                                                                      CCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCCTCCAGCCTTCAAGGGAGAACTGGGCCGTT                      >  SRR4302534.4181981/1‑94 (MQ=60)

                                                                      CCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTT                      <  SRR4302534.1006933/94‑1 (MQ=60)

                                                                      CCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTT                      <  SRR4302534.5737154/94‑1 (MQ=60)

                                                                       CAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTT                     >  SRR4302534.606899/1‑94 (MQ=60)

                                                                       CAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTT                     <  SRR4302534.5467033/94‑1 (MQ=60)

                                                                       CAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTT                     <  SRR4302534.5329427/94‑1 (MQ=60)

                                                                        AGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTC                    <  SRR4302534.3241542/94‑1 (MQ=60)

                                                                         GCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCC                   >  SRR4302534.6111909/1‑94 (MQ=60)

                                                                         GCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCC                   >  SRR4302534.2644035/1‑94 (MQ=60)

                                                                         GCTGCCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCC                   <  SRR4302534.3968505/94‑1 (MQ=60)

                                                                          CTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCA                  <  SRR4302534.694243/94‑1 (MQ=60)

                                                                          CTGGCACCGCCCTG‑TTACAGCCATACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGATCTGTGCCGTTTCCA                  <  SRR4302534.2406140/94‑1 (MQ=60)

                                                                           cgcCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTGCATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAG                 <  SRR4302534.4595451/91‑1 (MQ=60)

                                                                             GCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCG               <  SRR4302534.4424349/94‑1 (MQ=60)

                                                                               ACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAAGGAGAACTGTGCCGTTTCCAGCGCA             >  SRR4302534.5408799/1‑94 (MQ=60)

                                                                                CCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAA            <  SRR4302534.6165410/94‑1 (MQ=60)

                                                                                CCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAA            <  SRR4302534.5573291/94‑1 (MQ=60)

                                                                                 CGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTCCCGTTTCCAGCCCAAT           >  SRR4302534.1781701/1‑94 (MQ=60)

                                                                                  GCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA          >  SRR4302534.3600965/1‑94 (MQ=60)

                                                                                  GCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGGTTCCAGCGCAATA          >  SRR4302534.6264057/1‑94 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGAGGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        <  SRR4302534.178855/94‑1 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        >  SRR4302534.5922345/1‑94 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        >  SRR4302534.1607731/1‑94 (MQ=60)

                                                                                     CTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCACGGGAGCACTGTGCCGTTTCCCTCGCGATATTG       >  SRR4302534.5896700/1‑94 (MQ=60)

                                                                                     CTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG       >  SRR4302534.4309800/1‑94 (MQ=60)

                                                                                     CTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG       <  SRR4302534.204367/94‑1 (MQ=60)

                                                                                     CTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG       >  SRR4302534.2060046/1‑94 (MQ=60)

                                                                                       tgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT      >  SRR4302534.3083772/3‑94 (MQ=60)

                                                                                        GTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTC     >  SRR4302534.5260584/1‑94 (MQ=60)

                                                                                          TTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCT   <  SRR4302534.2040413/94‑1 (MQ=60)

                                                                                          TTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCT   <  SRR4302534.4088421/94‑1 (MQ=60)

                                                                                          TTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCT   <  SRR4302534.1451184/94‑1 (MQ=60)

                                                                                           TCAGCCAGCCGTCACCGCCGTTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGACCTGTGCCGTTCCCAGCGCAATATTGTCCTG  <  SRR4302534.2488547/94‑1 (MQ=60)

                                                                                           TCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG  >  SRR4302534.4171307/1‑94 (MQ=60)

                                                                                           TCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTACTG  >  SRR4302534.658942/1‑94 (MQ=60)

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG  >  NC_000913/3393015‑3393199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`