Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,410,599	A→G	G168G (GGT→GGC)	envR ←	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,410,599	0	A	G	99.6%	849.8 / ‑4.7	244	G168G (GGT→GGC)	envR	putative transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (137/106); total (138/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 246 total aligned reads displayed.

TAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGT  >  NC_000913/3410509‑3410688

 aaTACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCa                                                                                        >  2:5309976/1‑93 (MQ=255)

  aTACTTTATCGACCAGAGGGGGGGTTTGTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCAt                                                                                       <  2:3137945/93‑1 (MQ=255)

  aTACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCAt                                                                                       >  2:323567/1‑93 (MQ=255)

   tACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATc                                                                                      >  1:111683/1‑93 (MQ=255)

   tACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATc                                                                                      >  2:250251/1‑93 (MQ=255)

   tACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATc                                                                                      >  2:2450168/1‑93 (MQ=255)

   tACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATc                                                                                      >  1:4155963/1‑93 (MQ=255)

        tATCGACCAGAGGGGGGGCTGTTTTATAAAGATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCaataa                                                                                 <  2:2015195/93‑1 (MQ=255)

         aTCGACCAGAGGGGGGGCTTGTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCaataat                                                                                <  2:2358186/93‑1 (MQ=255)

         aTCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCaataaa                                                                                >  2:4101903/1‑92 (MQ=255)

          tCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCaataata                                                                               >  2:779594/1‑93 (MQ=255)

             aCCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAatc                                                                            >  1:2701982/1‑93 (MQ=255)

                agagCGGGGGTTTTTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAatcatc                                                                         <  2:920978/93‑1 (MQ=255)

                 gagCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAAtcatca                                                                        >  1:4509709/1‑93 (MQ=255)

                   gCGGGGTTTTGTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATcaca                                                                      <  1:5542689/93‑1 (MQ=255)

                     gggggTTTGTTAAAAAAAATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCacaac                                                                    <  1:2427275/93‑1 (MQ=255)

                      ggggCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCacaaca                                                                   >  1:5680629/1‑93 (MQ=255)

                      ggggCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCacaaca                                                                   >  2:1691679/1‑93 (MQ=255)

                        ggCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATc                                                                 >  1:5053918/1‑93 (MQ=255)

                         tcTTTTTTATAAACAGCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCt                                                                <  1:590910/92‑1 (MQ=255)

                          tttttttATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTa                                                               <  1:4345828/89‑1 (MQ=255)

                          cTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTa                                                               >  2:5251908/1‑93 (MQ=255)

                           ttGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTaa                                                              >  2:3344583/1‑93 (MQ=255)

                             ttttAAAAAGAACATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAAt                                                            <  2:4380733/92‑1 (MQ=255)

                              tttATAAAACTCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATc                                                           <  2:1345965/93‑1 (MQ=255)

                               ttATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCg                                                          <  1:289903/93‑1 (MQ=255)

                                taaaaaGATCACACGCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGa                                                         <  1:3023548/90‑1 (MQ=255)

                                 ataAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAg                                                        >  2:2190272/1‑93 (MQ=255)

                                   aaaTATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGt                                                      <  1:2534860/93‑1 (MQ=255)

                                   aaaGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGt                                                      <  2:5432365/93‑1 (MQ=255)

                                     aGATCATCCCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTa                                                    <  1:2770130/93‑1 (MQ=255)

                                     aGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTa                                                    >  1:1040316/1‑93 (MQ=255)

                                     aGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTa                                                    >  2:1821238/1‑93 (MQ=255)

                                     aGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTa                                                    >  2:4893887/1‑93 (MQ=255)

                                      gATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt                                                   <  1:6426549/93‑1 (MQ=255)

                                      gATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCCCTGAAGGCGCCATCAATAATCATCATCACAACATCTCAATCGTGGTTAt                                                   >  1:2171895/1‑93 (MQ=255)

                                      aaTCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt                                                   <  2:661818/92‑1 (MQ=255)

                                      aaTCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt                                                   <  2:4312345/92‑1 (MQ=255)

                                      aaTCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt                                                   <  2:2995412/92‑1 (MQ=255)

                                      aaTCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt                                                   <  2:1198479/92‑1 (MQ=255)

                                       ataaTACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAtt                                                  <  1:5570607/90‑1 (MQ=255)

                                        tCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAttt                                                 >  1:756149/1‑93 (MQ=255)

                                        tCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAttt                                                 >  1:5094024/1‑93 (MQ=255)

                                         cATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTg                                                <  1:5797750/93‑1 (MQ=255)

                                         cATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTg                                                >  1:99487/1‑93 (MQ=255)

                                         aaTACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTg                                                <  2:3641522/92‑1 (MQ=255)

                                           aaccccGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCt                                              <  2:571145/90‑1 (MQ=255)

                                           aaccccGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCt                                              <  2:2301039/90‑1 (MQ=255)

                                            aaCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTa                                             >  2:4189114/1‑93 (MQ=255)

                                              cccGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTaca                                           >  2:1619810/1‑93 (MQ=255)

                                              cccGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTaca                                           >  1:6143387/1‑93 (MQ=255)

                                               ccGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTacac                                          <  2:288323/93‑1 (MQ=255)

                                                  ccATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAAc                                       >  1:5147197/1‑93 (MQ=255)

                                                   cATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAAcc                                      >  2:3915140/1‑93 (MQ=255)

                                                     tatTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCtt                                    <  1:4022680/93‑1 (MQ=255)

                                                       ttCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttgt                                  >  2:4608420/1‑93 (MQ=255)

                                                       ttCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttgt                                  <  2:1967976/93‑1 (MQ=255)

                                                        tCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttgtt                                 >  2:1954712/1‑93 (MQ=255)

                                                         cATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTATACAACCttgttg                                >  2:5748097/1‑93 (MQ=255)

                                                         cATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttgttg                                <  1:2382094/93‑1 (MQ=255)

                                                          aTTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGc                               >  1:3608932/1‑93 (MQ=255)

                                                          aTTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGc                               >  2:5879963/1‑93 (MQ=255)

                                                          aTTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGc                               <  1:4987010/93‑1 (MQ=255)

                                                           ttAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCt                              >  2:5006212/1‑93 (MQ=255)

                                                           ttAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCt                              >  2:5539813/1‑93 (MQ=255)

                                                           ttAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCt                              <  1:1819664/93‑1 (MQ=255)

                                                             aaCCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGa                            <  1:1439711/93‑1 (MQ=255)

                                                             aaCCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGa                            >  1:3399419/1‑93 (MQ=255)

                                                               ccAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGaca                          >  1:839331/1‑93 (MQ=255)

                                                                cAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGacac                         >  1:3217260/1‑93 (MQ=255)

                                                                cAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGacac                         <  1:4826319/93‑1 (MQ=255)

                                                                  gTTTTGATCAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGc                       <  2:4682358/93‑1 (MQ=255)

                                                                  gTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGc                       >  2:4471936/1‑93 (MQ=255)

                                                                   ttttGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGcc                      <  1:6031235/93‑1 (MQ=255)

                                                                   ttttGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGcc                      >  1:4660503/1‑93 (MQ=255)

                                                                   ttttGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGcc                      <  1:712261/93‑1 (MQ=255)

                                                                       gAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCa                  <  2:2726486/93‑1 (MQ=255)

                                                                       gAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCa                  <  1:3533397/93‑1 (MQ=255)

                                                                        aaCAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCaa                 >  1:2962942/1‑93 (MQ=255)

                                                                        aaCAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCaa                 <  2:621158/93‑1 (MQ=255)

                                                                          cAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATa               <  2:3563137/93‑1 (MQ=255)

                                                                           aaTTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATAc              >  2:3051759/1‑93 (MQ=255)

                                                                           aaTTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTGGTTGCTGACACGCCTGCAATAc              <  2:3766985/93‑1 (MQ=255)

                                                                             ttCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACtt            <  2:5464723/93‑1 (MQ=255)

                                                                             tgccGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACtt            >  1:6370299/3‑93 (MQ=255)

                                                                              tCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTc           >  2:5065440/1‑93 (MQ=255)

                                                                              tCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTc           <  2:5902000/93‑1 (MQ=255)

                                                                               ccGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTcg          >  1:5403714/1‑93 (MQ=255)

                                                                               ccGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTcg          <  2:4430645/93‑1 (MQ=255)

                                                                                cGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTcgc         >  1:1921737/1‑93 (MQ=255)

                                                                                cGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTcgc         <  1:2117440/93‑1 (MQ=255)

                                                                                  cTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCgg       <  2:2633659/93‑1 (MQ=255)

                                                                                  cTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCgg       >  1:5211220/1‑93 (MQ=255)

                                                                                   tGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGga      <  2:3396528/93‑1 (MQ=255)

                                                                                   tGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGga      <  2:2618449/93‑1 (MQ=255)

                                                                                    gAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGgag     <  1:4312244/93‑1 (MQ=255)

                                                                                     aaGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGgaga    >  1:2855119/1‑93 (MQ=255)

                                                                                      aGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGagag   >  2:2816037/1‑93 (MQ=255)

                                                                                      aGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGagag   >  2:6024111/1‑93 (MQ=255)

                                                                                      aGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGagag   >  2:854196/1‑93 (MQ=255)

TAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGT  >  NC_000913/3410509‑3410688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCTGC  >  NC_000913/3410506‑3410692

tcttagtaggtaatgtcagcgaggggagatttgtttaaaaaatccatacCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCG                                                                                               <  SRR4302534.220566/45‑1 (MQ=60)

 CTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGC                                                                                              >  SRR4302534.4540607/1‑94 (MQ=60)

 CTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGC                                                                                              >  SRR4302534.3830060/1‑94 (MQ=60)

  TTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCC                                                                                             >  SRR4302534.5877437/1‑94 (MQ=60)

  TAAATACTTTATCGACCAGAGCGGGGGTTTTTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCC                                                                                             <  SRR4302534.5863626/94‑1 (MQ=60)

   TAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCA                                                                                            >  SRR4302534.5056534/1‑94 (MQ=60)

     ATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATC                                                                                          >  SRR4302534.4268173/1‑94 (MQ=60)

     ATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATC                                                                                          >  SRR4302534.114666/1‑94 (MQ=60)

      TACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCA                                                                                         >  SRR4302534.5716661/1‑94 (MQ=60)

      TACGTTATCGACAAGGGCGGGGTCTTTTTTAAAAAAATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCA                                                                                         <  SRR4302534.68204/94‑1 (MQ=60)

       ACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAA                                                                                        >  SRR4302534.4500727/1‑94 (MQ=60)

         TTTATCGACCAGAGCGGGGTTTTGTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATA                                                                                      <  SRR4302534.2228599/94‑1 (MQ=60)

         GTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATA                                                                                      >  SRR4302534.1939750/1‑94 (MQ=60)

         GTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATA                                                                                      >  SRR4302534.443606/1‑94 (MQ=60)

         GTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATA                                                                                      >  SRR4302534.4365780/1‑94 (MQ=60)

              CGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAAT                                                                                 >  SRR4302534.2070907/1‑94 (MQ=60)

              CGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAAT                                                                                 >  SRR4302534.258507/1‑94 (MQ=60)

               GACCAGAGCGGGGGTTTTTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATC                                                                                <  SRR4302534.5343931/94‑1 (MQ=60)

               GACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATC                                                                                >  SRR4302534.1144410/1‑94 (MQ=60)

               GACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATC                                                                                >  SRR4302534.2773351/1‑94 (MQ=60)

                ACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCA                                                                               >  SRR4302534.2530587/1‑94 (MQ=60)

                   AGAGCGGGGGCTTGTTTATATAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCA                                                                            >  SRR4302534.6405611/1‑94 (MQ=60)

                   AGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCA                                                                            >  SRR4302534.6000539/1‑94 (MQ=60)

                   AGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCA                                                                            >  SRR4302534.4631829/1‑94 (MQ=60)

                   AGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCA                                                                            >  SRR4302534.5319438/1‑94 (MQ=60)

                   AGAGCGGGGGCTTGTTTATAAAGATCATAAACCGCCATATTCATTAACCAGTTTTGAACAATTCCGATGAAGGCGCCATCAATAATAATCATCA                                                                            >  SRR4302534.5209939/1‑94 (MQ=60)

                    ggggggggtttttTTTATAAAGATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCAC                                                                           <  SRR4302534.4553565/81‑1 (MQ=60)

                      GCGGGGTTTTGTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAA                                                                         <  SRR4302534.5692300/94‑1 (MQ=60)

                      GCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAA                                                                         >  SRR4302534.523983/1‑94 (MQ=60)

                       CGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAAC                                                                        >  SRR4302534.1657758/1‑94 (MQ=60)

                        GGGGGTTTGTTAAAAAAAATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACA                                                                       <  SRR4302534.2491814/94‑1 (MQ=60)

                        GGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACA                                                                       >  SRR4302534.3033848/1‑94 (MQ=60)

                        GGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACA                                                                       >  SRR4302534.5834446/1‑94 (MQ=60)

                         GGGGTTTGTTTAAAAAAATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACAT                                                                      <  SRR4302534.608650/94‑1 (MQ=60)

                          GGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATC                                                                     >  SRR4302534.5190417/1‑94 (MQ=60)

                            tcttttttataaacagcaaacCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTA                                                                   <  SRR4302534.605331/73‑1 (MQ=60)

                             ttttTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAA                                                                  <  SRR4302534.4463185/90‑1 (MQ=60)

                             CTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAA                                                                  >  SRR4302534.3042780/1‑94 (MQ=60)

                                ttttaaaaaaataatacCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATC                                                               <  SRR4302534.4079845/77‑1 (MQ=60)

                                  TTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGA                                                             <  SRR4302534.297401/94‑1 (MQ=60)

                                   taaaaagatcacacgCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAG                                                            <  SRR4302534.3102775/79‑1 (MQ=60)

                                   taaaaaaaacatacCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAG                                                            <  SRR4302534.4228320/80‑1 (MQ=60)

                                   aaaaaaaATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAG                                                            <  SRR4302534.5809792/87‑1 (MQ=60)

                                   TATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAG                                                            >  SRR4302534.6568743/1‑94 (MQ=60)

                                    aaaaggataatacCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGG                                                           <  SRR4302534.4755092/81‑1 (MQ=60)

                                    ATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGG                                                           >  SRR4302534.5137627/1‑94 (MQ=60)

                                    ATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGG                                                           >  SRR4302534.4065966/1‑94 (MQ=60)

                                    ATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGG                                                           >  SRR4302534.8585/1‑94 (MQ=60)

                                     TAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGT                                                          >  SRR4302534.5806392/1‑94 (MQ=60)

                                      AAATATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTT                                                         <  SRR4302534.2602143/94‑1 (MQ=60)

                                      AAAGATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTT                                                         <  SRR4302534.2740612/94‑1 (MQ=60)

                                      AAAGACAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGTTT                                                         <  SRR4302534.4593667/94‑1 (MQ=60)

                                       aaaataaaacCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTA                                                        <  SRR4302534.1647592/84‑1 (MQ=60)

                                       AAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTA                                                        >  SRR4302534.1067167/1‑94 (MQ=60)

                                        AGATCATCCCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTAT                                                       <  SRR4302534.2843037/94‑1 (MQ=60)

                                        AGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCCCTGAAGGCGCCATCAATAATCATCATCACAACATCTCAATCGTGGTTAT                                                       >  SRR4302534.2229266/1‑94 (MQ=60)

                                         gccaaaacCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATT                                                      <  SRR4302534.6191920/86‑1 (MQ=60)

                                         GATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATT                                                      <  SRR4302534.6599859/94‑1 (MQ=60)

                                         AATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATT                                                      <  SRR4302534.6068100/94‑1 (MQ=60)

                                          ATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTT                                                     >  SRR4302534.5231526/1‑94 (MQ=60)

                                          ATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTT                                                     >  SRR4302534.774898/1‑94 (MQ=60)

                                          ATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTT                                                     <  SRR4302534.5721034/94‑1 (MQ=60)

                                           TCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTG                                                    >  SRR4302534.102142/1‑94 (MQ=60)

                                            CATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGC                                                   <  SRR4302534.5955071/94‑1 (MQ=60)

                                            CATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGC                                                   >  SRR4302534.2774918/1‑94 (MQ=60)

                                             ATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCT                                                  >  SRR4302534.2817781/1‑94 (MQ=60)

                                                ACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACA                                               >  SRR4302534.6310470/1‑94 (MQ=60)

                                                  CCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACA                                             <  SRR4302534.2451243/94‑1 (MQ=60)

                                                  CCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACA                                             >  SRR4302534.2856479/1‑94 (MQ=60)

                                                   CGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAA                                            >  SRR4302534.6504707/1‑94 (MQ=60)

                                                   CGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAA                                            >  SRR4302534.918457/1‑94 (MQ=60)

                                                   CGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAA                                            >  SRR4302534.2931247/1‑94 (MQ=60)

                                                    GCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAAC                                           >  SRR4302534.5286080/1‑94 (MQ=60)

                                                    GCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAAA                                           <  SRR4302534.4267387/94‑1 (MQ=60)

                                                      CATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCT                                         <  SRR4302534.3449583/94‑1 (MQ=60)

                                                       ATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTT                                        >  SRR4302534.130552/1‑94 (MQ=60)

                                                       ATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTT                                        <  SRR4302534.739588/94‑1 (MQ=60)

                                                        TATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTG                                       >  SRR4302534.3185708/1‑94 (MQ=60)

                                                        TATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTG                                       >  SRR4302534.2294075/1‑94 (MQ=60)

                                                        TATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTG                                       <  SRR4302534.4130538/94‑1 (MQ=60)

                                                          TTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTT                                     <  SRR4302534.4757914/94‑1 (MQ=60)

                                                           TCATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTG                                    >  SRR4302534.5678102/1‑94 (MQ=60)

                                                           TCATGAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTG                                    <  SRR4302534.1523985/94‑1 (MQ=60)

                                                            CATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGC                                   >  SRR4302534.3705979/1‑94 (MQ=60)

                                                            CATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGC                                   <  SRR4302534.2445389/94‑1 (MQ=60)

                                                             ATTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCT                                  <  SRR4302534.5121952/94‑1 (MQ=60)

                                                              TTAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTG                                 <  SRR4302534.1866957/94‑1 (MQ=60)

                                                               TAACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGA                                >  SRR4302534.3490666/1‑94 (MQ=60)

                                                                AACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGAC                               <  SRR4302534.1477607/94‑1 (MQ=60)

                                                                 ACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTACATCGAGGGTATTTGCTACACAACCTTGTTGCTGACA                              >  SRR4302534.6619877/1‑94 (MQ=60)

                                                                 ACCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACA                              >  SRR4302534.860470/1‑94 (MQ=60)

                                                                  CCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACAC                             >  SRR4302534.807767/1‑94 (MQ=60)

                                                                  CCAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACAC                             >  SRR4302534.3302650/1‑94 (MQ=60)

                                                                   CAGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACG                            <  SRR4302534.4957290/94‑1 (MQ=60)

                                                                    AGTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGC                           >  SRR4302534.3028115/1‑94 (MQ=60)

                                                                     GTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCC                          >  SRR4302534.4787036/1‑94 (MQ=60)

                                                                     GTTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCC                          >  SRR4302534.6262437/1‑94 (MQ=60)

                                                                      TTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCT                         <  SRR4302534.6195126/94‑1 (MQ=60)

                                                                      TTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCT                         <  SRR4302534.729769/94‑1 (MQ=60)

                                                                      TTTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCT                         <  SRR4302534.4425230/94‑1 (MQ=60)

                                                                       TTTGAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTG                        >  SRR4302534.1983813/1‑94 (MQ=60)

                                                                          GAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAA                     >  SRR4302534.3040546/1‑94 (MQ=60)

                                                                          GAACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAA                     <  SRR4302534.3628447/94‑1 (MQ=60)

                                                                            ACAATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATA                   <  SRR4302534.3567015/94‑1 (MQ=60)

                                                                               ATTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTT                >  SRR4302534.5535043/1‑94 (MQ=60)

                                                                               ATGCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTT                >  SRR4302534.6542294/1‑94 (MQ=60)

                                                                                TTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTC               <  SRR4302534.5011282/94‑1 (MQ=60)

                                                                                TTCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTC               <  SRR4302534.5582823/94‑1 (MQ=60)

                                                                                 TCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCG              >  SRR4302534.5549145/1‑94 (MQ=60)

                                                                                 TCCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCG              <  SRR4302534.3517876/94‑1 (MQ=60)

                                                                                  CCGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGC             >  SRR4302534.1971975/1‑94 (MQ=60)

                                                                                   CGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCG            <  SRR4302534.2173168/94‑1 (MQ=60)

                                                                                   CGCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCG            <  SRR4302534.319975/94‑1 (MQ=60)

                                                                                    GCTGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGG           >  SRR4302534.5351863/1‑94 (MQ=60)

                                                                                      TGAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAG         >  SRR4302534.485734/1‑94 (MQ=60)

                                                                                       GAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGA        >  SRR4302534.2930109/1‑94 (MQ=60)

                                                                                       GAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGA        <  SRR4302534.5193690/94‑1 (MQ=60)

                                                                                       GAAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGA        <  SRR4302534.4429064/94‑1 (MQ=60)

                                                                                        AAGGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAG       >  SRR4302534.2316233/1‑94 (MQ=60)

                                                                                          GGCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTC     <  SRR4302534.3646115/94‑1 (MQ=60)

                                                                                           GCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCT    <  SRR4302534.2300983/94‑1 (MQ=60)

                                                                                           GCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCT    <  SRR4302534.2331596/94‑1 (MQ=60)

                                                                                           GCGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCT    <  SRR4302534.2464810/94‑1 (MQ=60)

                                                                                            CGCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCTG   >  SRR4302534.3364104/1‑94 (MQ=60)

                                                                                             GCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCTGC  <  SRR4302534.6362431/94‑1 (MQ=60)

                                                                                             GCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCTGC  >  SRR4302534.2159099/1‑94 (MQ=60)

                                                                                             GCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCTGC  >  SRR4302534.3867657/1‑94 (MQ=60)

                                                                                             GCCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCTGC  <  SRR4302534.5100051/94‑1 (MQ=60)

TCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAGTCTGC  >  NC_000913/3410506‑3410692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`