Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,429,527 Δ1 bp coding (40/510 nt) yrdD ← putative DNA topoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,429,5260C.100.0% 1020.3 / NA 216coding (41/510 nt)yrdDputative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (94/122);  total (94/122)

Only 100 of 230 total aligned reads displayed.

CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614
                                                                                            |                                                                                        
cGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGCTACTGTGAGCATCCAAGAAACGGACAGTGTTTCCCGGATc                                                                                          >  2:3348682/1‑93 (MQ=255)
 gATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg                                                                                         <  2:5505864/93‑2 (MQ=255)
   tatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaa                                                                                       <  2:6441220/93‑4 (MQ=255)
          aTCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg                                                                               >  1:3332874/1‑93 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              <  2:4745940/93‑1 (MQ=255)
            ccGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGtt                                                                             <  1:3798670/93‑1 (MQ=255)
            ccGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGtt                                                                             <  1:2921829/93‑1 (MQ=255)
            ccGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGtt                                                                             >  1:2411081/1‑93 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            <  2:155974/93‑1 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            <  2:4231418/93‑1 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            <  1:2951529/93‑1 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            <  2:2336764/93‑1 (MQ=255)
              gCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa                                                                           <  2:2728635/93‑1 (MQ=255)
              gCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa                                                                           >  1:6061079/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        >  2:5613903/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        <  2:1827896/93‑1 (MQ=255)
                  aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGccc                                                                       <  1:3248609/93‑1 (MQ=255)
                   aGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc                                                                      >  2:476379/1‑93 (MQ=255)
                    gATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg                                                                     >  2:3360976/1‑93 (MQ=255)
                       ttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAc                                                                  <  2:4397281/93‑1 (MQ=255)
                       ttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAAAGGACCGTGTTTACCGGAT‑CGAATAACCAGTTCAGCCCCGCAc                                                                  >  1:3102928/1‑93 (MQ=255)
                        tCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACt                                                                 <  1:5955078/93‑1 (MQ=255)
                         cAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt                                                                <  1:199731/93‑1 (MQ=255)
                         cAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt                                                                <  1:5060410/93‑1 (MQ=255)
                         cAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt                                                                <  2:1769616/93‑1 (MQ=255)
                          agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt                                                               >  1:5602722/1‑93 (MQ=255)
                          agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt                                                               <  1:568273/93‑1 (MQ=255)
                          agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt                                                               <  1:5204532/93‑1 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              >  2:3228145/1‑93 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              >  1:3359341/1‑93 (MQ=255)
                            agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             >  1:4355848/1‑93 (MQ=255)
                            agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             >  1:5815642/1‑93 (MQ=255)
                             ggacggacGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg                                                            >  1:274612/1‑93 (MQ=255)
                               acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCa                                                          <  1:1785642/93‑1 (MQ=255)
                                cggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAg                                                         <  2:4719865/93‑1 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       >  2:5559447/1‑93 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       >  2:6053385/1‑93 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       <  2:800654/93‑1 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      >  2:4618000/1‑93 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      >  1:4918387/1‑93 (MQ=255)
                                     gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTc                                                    <  2:1189901/93‑1 (MQ=255)
                                      tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa                                                   <  1:4735723/93‑1 (MQ=255)
                                          cacaCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattat                                               >  1:823097/1‑93 (MQ=255)
                                             acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc                                            >  2:610313/1‑93 (MQ=255)
                                                ccGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCa                                         >  2:2759468/1‑93 (MQ=255)
                                                ccGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCa                                         <  1:1259407/93‑1 (MQ=255)
                                                ccGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCa                                         >  2:1449873/1‑93 (MQ=255)
                                                 cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc                                        >  1:4067255/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  2:3023027/93‑1 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       >  1:3890763/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       >  1:3487671/1‑93 (MQ=255)
                                                    ctcttgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCGCCGt                                     <  1:1522502/89‑1 (MQ=255)
                                                    aTACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt                                     <  1:2241522/93‑1 (MQ=255)
                                                      aCTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGa                                   >  1:4826496/1‑93 (MQ=255)
                                                      aCTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGa                                   >  2:1378516/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 <  2:4831884/93‑1 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 <  1:731795/93‑1 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  1:2585060/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                >  2:3137632/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:5583504/93‑1 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               <  1:450101/93‑1 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               <  2:632083/93‑1 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  1:1383505/1‑93 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            <  1:5289437/93‑1 (MQ=255)
                                                              cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACCGTGCt                           <  1:4186761/93‑1 (MQ=255)
                                                              cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt                           <  2:4303475/93‑1 (MQ=255)
                                                              cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt                           <  1:3013879/93‑1 (MQ=255)
                                                               aTCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTg                          >  2:4854592/1‑93 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         <  1:5531210/93‑1 (MQ=255)
                                                                 ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt                        <  1:6268678/93‑1 (MQ=255)
                                                                  cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAtt                       <  2:2165423/93‑1 (MQ=255)
                                                                  cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAtt                       <  1:2518127/93‑1 (MQ=255)
                                                                   aaGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt                      <  1:1448886/93‑1 (MQ=255)
                                                                   aaGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt                      <  1:2118280/93‑1 (MQ=255)
                                                                    aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTc                     <  2:5781985/93‑1 (MQ=255)
                                                                    aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTc                     <  1:788747/93‑1 (MQ=255)
                                                                     gAAACTGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    >  2:6172834/1‑93 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    <  2:1089426/93‑1 (MQ=255)
                                                                       aaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGcc                  <  2:3562436/93‑1 (MQ=255)
                                                                       aaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGcc                  >  2:4964944/1‑93 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCGCCGTGAACAGTGCTGATTTCGCCa                 <  1:1008918/93‑1 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 <  2:2254843/93‑1 (MQ=255)
                                                                         cGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAt                <  1:4907282/93‑1 (MQ=255)
                                                                         cGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAt                <  2:1910403/93‑1 (MQ=255)
                                                                           gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaa              >  1:6148026/1‑93 (MQ=255)
                                                                           gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaa              >  1:812349/1‑93 (MQ=255)
                                                                           gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaa              <  1:1891316/93‑1 (MQ=255)
                                                                            aCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaac             <  1:405582/93‑1 (MQ=255)
                                                                             ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaaca            >  1:1043245/1‑93 (MQ=255)
                                                                              cGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaa           <  1:6385828/93‑1 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          >  1:1657831/1‑93 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          >  2:397713/1‑93 (MQ=255)
                                                                                tgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACt         <  2:347476/93‑1 (MQ=255)
                                                                                 gtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACtt        >  2:76144/1‑93 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACTCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       >  2:5717486/1‑93 (MQ=255)
                                                                                   ttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAt      <  2:5303628/93‑1 (MQ=255)
                                                                                   ttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAt      <  1:1233284/93‑1 (MQ=255)
                                                                                    tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATg     <  2:2053853/93‑1 (MQ=255)
                                                                                    tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATg     <  1:913509/93‑1 (MQ=255)
                                                                                      ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCt   <  1:4387703/93‑1 (MQ=255)
                                                                                            |                                                                                        
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA  >  NC_000913/3429436‑3429619
                                                                                           |                                                                                            
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAA                                                                                           >  SRR4302534.5343586/1‑94 (MQ=60)
   TGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAA                                                                                        >  SRR4302534.3388420/1‑94 (MQ=60)
   TGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAA                                                                                        <  SRR4302534.5743976/94‑1 (MQ=60)
    GTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAC                                                                                       <  SRR4302534.5849432/94‑1 (MQ=60)
     TCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACC                                                                                      <  SRR4302534.2176051/94‑1 (MQ=60)
      CCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCA                                                                                     >  SRR4302534.2528934/1‑94 (MQ=60)
       CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                    >  SRR4302534.3422022/1‑94 (MQ=60)
       CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                    >  SRR4302534.4331719/1‑94 (MQ=60)
       CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                    >  SRR4302534.6286124/1‑94 (MQ=60)
        ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                   <  SRR4302534.6005055/94‑1 (MQ=60)
        ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                   >  SRR4302534.406755/1‑94 (MQ=60)
         TCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTT                                                                                  >  SRR4302534.2475196/1‑94 (MQ=60)
          CCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTC                                                                                 <  SRR4302534.3900667/94‑1 (MQ=60)
          CCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTC                                                                                 <  SRR4302534.2998509/94‑1 (MQ=60)
           CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                >  SRR4302534.6225885/1‑94 (MQ=60)
           CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                <  SRR4302534.2015030/94‑1 (MQ=60)
           CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                <  SRR4302534.3028923/94‑1 (MQ=60)
               GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCC                                                                            >  SRR4302534.5707777/1‑94 (MQ=60)
               GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCC                                                                            >  SRR4302534.1684040/1‑94 (MQ=60)
                AAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCC                                                                           <  SRR4302534.1817354/94‑1 (MQ=60)
                AAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCC                                                                           <  SRR4302534.3335115/94‑1 (MQ=60)
                   ATTTCAGAGGATGGACGTAGTCTCACGCCGGATACTGTGAGCATCCGAGAAACGGACCGGGTTTCGCGGAT‑CGAATAACCAGTTCAGCCCCGCC                                                                        >  SRR4302534.3975963/1‑94 (MQ=60)
                    TTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAAAGGACCGTGTTTACCGGAT‑CGAATAACCAGTTCAGCCCCGCAC                                                                       >  SRR4302534.3184554/1‑94 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                      <  SRR4302534.4201806/94‑1 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                      <  SRR4302534.41150/94‑1 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                      <  SRR4302534.5815159/94‑1 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                      <  SRR4302534.2745421/94‑1 (MQ=60)
                      TCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTT                                                                     <  SRR4302534.6116785/94‑1 (MQ=60)
                      TCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTT                                                                     <  SRR4302534.4444772/94‑1 (MQ=60)
                       CAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTT                                                                    >  SRR4302534.4904147/1‑94 (MQ=60)
                       CAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTT                                                                    <  SRR4302534.204973/94‑1 (MQ=60)
                       CAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTT                                                                    >  SRR4302534.5754244/1‑94 (MQ=60)
                       CAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTT                                                                    <  SRR4302534.5197072/94‑1 (MQ=60)
                        AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                   <  SRR4302534.582229/94‑1 (MQ=60)
                        AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                   >  SRR4302534.3449317/1‑94 (MQ=60)
                        AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                   <  SRR4302534.5344951/94‑1 (MQ=60)
                        AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                   >  SRR4302534.4885006/1‑94 (MQ=60)
                         GAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGG                                                                  >  SRR4302534.4473404/1‑94 (MQ=60)
                         GAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGG                                                                  >  SRR4302534.5973485/1‑94 (MQ=60)
                          AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                 >  SRR4302534.281720/1‑94 (MQ=60)
                          AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                 >  SRR4302534.3427934/1‑94 (MQ=60)
                             ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                              <  SRR4302534.1832003/94‑1 (MQ=60)
                             ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                              >  SRR4302534.416387/1‑94 (MQ=60)
                              CGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGG                                                             >  SRR4302534.792142/1‑94 (MQ=60)
                               GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                            >  SRR4302534.5150110/1‑94 (MQ=60)
                               GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCCCACTTTGGGCAGGA                                                            >  SRR4302534.3799957/1‑94 (MQ=60)
                                GACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAC                                                           >  SRR4302534.1443425/1‑94 (MQ=60)
                                GACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAC                                                           >  SRR4302534.5051690/1‑94 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                       <  SRR4302534.1500611/94‑1 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                       <  SRR4302534.4864301/94‑1 (MQ=60)
                                     AGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATT                                                      <  SRR4302534.5660544/94‑1 (MQ=60)
                                       TCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTAT                                                    >  SRR4302534.843806/1‑94 (MQ=60)
                                        CACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATT                                                   <  SRR4302534.4569414/94‑1 (MQ=60)
                                        CACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATT                                                   >  SRR4302534.6505057/1‑94 (MQ=60)
                                         ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                  >  SRR4302534.1673079/1‑94 (MQ=60)
                                          CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                 <  SRR4302534.5956110/94‑1 (MQ=60)
                                            CGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGC                                               >  SRR4302534.889743/1‑94 (MQ=60)
                                              CCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAC                                             <  SRR4302534.1292580/94‑1 (MQ=60)
                                              CCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAC                                             >  SRR4302534.4176381/1‑94 (MQ=60)
                                               CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                            >  SRR4302534.3995210/1‑94 (MQ=60)
                                               CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                            >  SRR4302534.832742/1‑94 (MQ=60)
                                               CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                            >  SRR4302534.2513590/1‑94 (MQ=60)
                                               CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                            >  SRR4302534.3581405/1‑94 (MQ=60)
                                                GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                           >  SRR4302534.3508543/1‑94 (MQ=60)
                                                 GATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGT                                          <  SRR4302534.3945723/94‑1 (MQ=60)
                                                  ctctTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCGCCGTG                                         <  SRR4302534.1562473/90‑1 (MQ=60)
                                                  ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                         <  SRR4302534.2300927/94‑1 (MQ=60)
                                                  ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTAAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                         >  SRR4302534.1231916/1‑94 (MQ=60)
                                                   TACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGA                                        >  SRR4302534.4957471/1‑94 (MQ=60)
                                                     CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                      >  SRR4302534.2653489/1‑94 (MQ=60)
                                                      TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                     <  SRR4302534.5107912/94‑1 (MQ=60)
                                                      TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                     <  SRR4302534.749881/94‑1 (MQ=60)
                                                       GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                    <  SRR4302534.5734375/94‑1 (MQ=60)
                                                        TGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGT                                   <  SRR4302534.461504/94‑1 (MQ=60)
                                                         GAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTG                                  <  SRR4302534.4835791/94‑1 (MQ=60)
                                                         GAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTG                                  >  SRR4302534.1055925/1‑94 (MQ=60)
                                                          AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                 >  SRR4302534.4497898/1‑94 (MQ=60)
                                                          AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                 >  SRR4302534.1419928/1‑94 (MQ=60)
                                                           GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                <  SRR4302534.4988059/94‑1 (MQ=60)
                                                           GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                <  SRR4302534.5432217/94‑1 (MQ=60)
                                                            CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACCGTGCTG                               <  SRR4302534.4299905/94‑1 (MQ=60)
                                                            CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                               <  SRR4302534.3235415/94‑1 (MQ=60)
                                                            CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                               <  SRR4302534.3092765/94‑1 (MQ=60)
                                                              TCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAT                             <  SRR4302534.5680509/94‑1 (MQ=60)
                                                               CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                            <  SRR4302534.6438421/94‑1 (MQ=60)
                                                               CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                            <  SRR4302534.2636734/94‑1 (MQ=60)
                                                                CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                           <  SRR4302534.2044883/94‑1 (MQ=60)
                                                                CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                           <  SRR4302534.2584986/94‑1 (MQ=60)
                                                                 AAGAAACGGACCGTGTTTCCCGGAT‑TGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                          >  SRR4302534.6395232/1‑94 (MQ=60)
                                                                 AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                          <  SRR4302534.1487018/94‑1 (MQ=60)
                                                                 AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                          <  SRR4302534.2174036/94‑1 (MQ=60)
                                                                  AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                         <  SRR4302534.808497/94‑1 (MQ=60)
                                                                     AACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCTCCGTGAACAGTGCTGATTTCGCCA                      <  SRR4302534.1378112/94‑1 (MQ=60)
                                                                      ACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCGCCGTGAACAGTGCTGATTTCGCCAT                     <  SRR4302534.1034836/94‑1 (MQ=60)
                                                                      ACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAT                     <  SRR4302534.2953123/94‑1 (MQ=60)
                                                                       CGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATA                    <  SRR4302534.5040303/94‑1 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                   >  SRR4302534.2888164/1‑94 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                   >  SRR4302534.84038/1‑94 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                   >  SRR4302534.6315240/1‑94 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                   >  SRR4302534.832759/1‑94 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                   <  SRR4302534.6192077/94‑1 (MQ=60)
                                                                         GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAAC                  <  SRR4302534.3020175/94‑1 (MQ=60)
                                                                         GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAAC                  <  SRR4302534.1940643/94‑1 (MQ=60)
                                                                          ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                 >  SRR4302534.1070185/1‑94 (MQ=60)
                                                                          ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                 <  SRR4302534.415948/94‑1 (MQ=60)
                                                                          ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                 >  SRR4302534.6256050/1‑94 (MQ=60)
                                                                          ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGGGCTGATTTCGCCATAACA                 >  SRR4302534.3473217/1‑94 (MQ=60)
                                                                           CCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAA                >  SRR4302534.6638395/1‑94 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC               <  SRR4302534.6558332/94‑1 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC               >  SRR4302534.1701024/1‑94 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC               >  SRR4302534.1937015/1‑94 (MQ=60)
                                                                             GTGTTTCCCGGAT‑GGAATAACCAGTTCAGCCACGCGCTTTGGGCAGGACTCATTATTCCGCACCGTGAACAGTGCTGATTTCGCCATAACAACT              <  SRR4302534.1346095/94‑1 (MQ=60)
                                                                             GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT              <  SRR4302534.5471391/94‑1 (MQ=60)
                                                                               GTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTA            <  SRR4302534.66949/94‑1 (MQ=60)
                                                                                TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT           <  SRR4302534.2358118/94‑1 (MQ=60)
                                                                                 TTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG          <  SRR4302534.1265782/94‑1 (MQ=60)
                                                                                 TTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG          <  SRR4302534.3116445/94‑1 (MQ=60)
                                                                                 TTCCCGGAT‑CGAATAACCAGTTCACCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG          <  SRR4302534.215770/94‑1 (MQ=60)
                                                                                  TCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGC         <  SRR4302534.936745/94‑1 (MQ=60)
                                                                                  TCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGC         <  SRR4302534.3184271/94‑1 (MQ=60)
                                                                                   CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT        <  SRR4302534.5420569/94‑1 (MQ=60)
                                                                                   CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT        <  SRR4302534.894727/94‑1 (MQ=60)
                                                                                   CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT        >  SRR4302534.3065378/1‑94 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG       <  SRR4302534.1276961/94‑1 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG       <  SRR4302534.4506007/94‑1 (MQ=60)
                                                                                      GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAA     >  SRR4302534.888137/1‑94 (MQ=60)
                                                                                       GAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAAT    >  SRR4302534.4402650/1‑94 (MQ=60)
                                                                                          TCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA  <  SRR4302534.1983332/94‑1 (MQ=60)
                                                                                          TCGAATAACCAGTTCAGCCCCGCACTTTGGGCACGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA  <  SRR4302534.3435707/94‑1 (MQ=60)
                                                                                           |                                                                                            
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA  >  NC_000913/3429436‑3429619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑