Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,509,198	A→G	V189A (GTG→GCG)	yhfZ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,509,198	0	A	G	99.2%	834.6 / ‑3.2	246	V189A (GTG→GCG)	yhfZ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (108/136); total (109/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 254 total aligned reads displayed.

ACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACG  >  NC_000913/3509108‑3509287

aCCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACcgcg                                                                                         <  1:4540429/93‑1 (MQ=255)

 cccGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCgg                                                                                        >  1:2196428/1‑93 (MQ=255)

 cccGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCgg                                                                                        >  2:1530733/1‑93 (MQ=255)

   cGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTTTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCt                                                                                      >  1:5321348/1‑93 (MQ=255)

   cGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCt                                                                                      <  2:540539/93‑1 (MQ=255)

   cGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCt                                                                                      >  1:1542517/1‑93 (MQ=255)

   cGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCt                                                                                      <  1:5621652/93‑1 (MQ=255)

    gttgttGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCtt                                                                                     <  2:5001569/93‑1 (MQ=255)

    gttgttGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCtt                                                                                     <  2:2003014/93‑1 (MQ=255)

     ttgttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTc                                                                                    >  2:4052680/1‑93 (MQ=255)

     ttgttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTc                                                                                    <  1:4980528/93‑1 (MQ=255)

     ttgttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTc                                                                                    >  1:2010081/1‑93 (MQ=255)

     ttgttgATGGGCGAGCAGGGCGTATTTATCTACAACGGCACGCATCAGTTCTTCCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTc                                                                                    <  2:382309/93‑1 (MQ=255)

       gttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCgg                                                                                  <  2:3549742/93‑1 (MQ=255)

        ttgATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGt                                                                                 <  1:5751260/93‑1 (MQ=255)

          gATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTgg                                                                               >  2:4700817/1‑93 (MQ=255)

            tGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGcc                                                                             <  1:3242271/93‑1 (MQ=255)

             gggCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCt                                                                            >  1:4193060/1‑93 (MQ=255)

                     aGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAAt                                                                    >  1:6418980/1‑93 (MQ=255)

                     aGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAAt                                                                    >  1:3319032/1‑93 (MQ=255)

                        gCGTGTTTATCTACAACGGCACGCATCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATcgc                                                                 <  1:5515421/93‑1 (MQ=255)

                        gCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCTGCTTCGGTGGCCTGTAAAAATcgc                                                                 <  1:3110634/93‑1 (MQ=255)

                          gtgtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCgg                                                               >  1:5474393/1‑93 (MQ=255)

                          gtgtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCgg                                                               >  1:5761573/1‑93 (MQ=255)

                           tgtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGa                                                              <  1:4830223/93‑1 (MQ=255)

                            gtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGAt                                                             <  1:6172359/93‑1 (MQ=255)

                            gtTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGAt                                                             >  2:6452638/1‑93 (MQ=255)

                             tttATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATTATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATc                                                            <  2:4641877/93‑1 (MQ=255)

                             tttATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCAGATc                                                            >  2:1917078/1‑93 (MQ=255)

                              ttATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCg                                                           <  1:2672116/93‑1 (MQ=255)

                                aTCTACACCGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTc                                                         <  1:2391897/93‑1 (MQ=255)

                                aTCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTc                                                         <  1:4478909/93‑1 (MQ=255)

                                aTCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTc                                                         >  2:1807779/1‑93 (MQ=255)

                                 tCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCt                                                        >  2:4476488/1‑93 (MQ=255)

                                  cTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtg                                                       <  1:5126413/93‑1 (MQ=255)

                                  cTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtg                                                       >  2:2814078/1‑93 (MQ=255)

                                   tACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgt                                                      >  2:4605119/1‑93 (MQ=255)

                                    gcAACGTCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACGGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgtg                                                     <  1:3632653/92‑1 (MQ=255)

                                    aCAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgtg                                                     <  2:1707814/93‑1 (MQ=255)

                                     cAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGa                                                    >  1:4444899/1‑93 (MQ=255)

                                      aaCGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAg                                                   <  2:6428800/93‑1 (MQ=255)

                                      aaCGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAg                                                   >  1:3155435/1‑93 (MQ=255)

                                       aCGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAgc                                                  <  1:2593831/93‑1 (MQ=255)

                                        cGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAgcg                                                 <  1:2979056/93‑1 (MQ=255)

                                         ggCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACAGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCATCTGTGAgcgg                                                >  2:5124102/1‑93 (MQ=255)

                                            aCGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGt                                             <  2:4089074/93‑1 (MQ=255)

                                            aCGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGt                                             >  1:1008423/1‑93 (MQ=255)

                                            aCGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGt                                             >  2:5054213/1‑93 (MQ=255)

                                            aCGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGt                                             <  1:6115619/93‑1 (MQ=255)

                                              gcagcaGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGGGGATCAGCTGGGCGCGGGGTCg                                           >  1:1613695/1‑93 (MQ=255)

                                              gcagcaGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGCgcggcggcg                                           >  2:4950883/1‑93 (MQ=255)

                                              gcagcaGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCg                                           <  1:4299183/93‑1 (MQ=255)

                                               cagcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGc                                          <  2:4544743/93‑1 (MQ=255)

                                                agcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGTCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGcc                                         <  1:1444417/93‑1 (MQ=255)

                                                agcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGcc                                         >  1:5956508/1‑93 (MQ=255)

                                                 gcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCct                                        <  2:5956445/93‑1 (MQ=255)

                                                 gcagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCct                                        <  2:2975103/93‑1 (MQ=255)

                                                  cagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCctc                                       <  2:515484/93‑1 (MQ=255)

                                                  cagTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCctc                                       <  1:3696320/93‑1 (MQ=255)

                                                   agTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCctct                                      <  1:1116900/93‑1 (MQ=255)

                                                    gttgttGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTa                                     >  1:1391157/1‑93 (MQ=255)

                                                     ttgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                                    >  1:2650204/1‑93 (MQ=255)

                                                     ttgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                                    <  2:4320509/93‑1 (MQ=255)

                                                     ttgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                                    >  1:2135881/1‑93 (MQ=255)

                                                     ttgttgCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                                    <  2:743718/93‑1 (MQ=255)

                                                          gCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTTATCCCa                               >  1:5586691/1‑93 (MQ=255)

                                                            aTTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGc                             >  2:3770280/1‑93 (MQ=255)

                                                               gggTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATg                          >  2:139733/1‑93 (MQ=255)

                                                                ggTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATgg                         <  1:1357753/93‑1 (MQ=255)

                                                                ggTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATgg                         <  2:6233581/93‑1 (MQ=255)

                                                                 gTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGGCTCTAATCCCAGCATGGt                        >  1:2934586/1‑93 (MQ=255)

                                                                  tAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTc                       >  2:427197/1‑93 (MQ=255)

                                                                   aaTCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCa                      <  2:2224240/93‑1 (MQ=255)

                                                                   aaTCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCa                      >  2:2545815/1‑93 (MQ=255)

                                                                    atcatcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGAGTCGCCTCTAATCCCAGCATGGTCAg                     >  2:3871594/1‑93 (MQ=255)

                                                                      catcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGtt                   >  2:638939/1‑93 (MQ=255)

                                                                       atcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTc                  <  2:5270235/93‑1 (MQ=255)

                                                                       atcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTc                  >  1:521808/1‑93 (MQ=255)

                                                                        tcGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATAGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCg                 >  1:2818175/1‑93 (MQ=255)

                                                                         cGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGt                >  1:861188/1‑93 (MQ=255)

                                                                         cGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGt                <  2:301841/93‑1 (MQ=255)

                                                                         cGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGt                <  1:387155/93‑1 (MQ=255)

                                                                          gACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGtt               >  2:3924688/1‑93 (MQ=255)

                                                                           gcTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt              <  1:4001336/92‑1 (MQ=255)

                                                                           aCTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt              <  2:1034296/93‑1 (MQ=255)

                                                                           aCTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt              >  2:199612/1‑93 (MQ=255)

                                                                           aCTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt              <  1:1876123/93‑1 (MQ=255)

                                                                             tCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTc            <  1:2500210/93‑1 (MQ=255)

                                                                              cgcgTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTcc           <  2:3390753/93‑1 (MQ=255)

                                                                              cgcgTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTcc           <  2:1637070/93‑1 (MQ=255)

                                                                              cgcgTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTcc           <  2:1267710/93‑1 (MQ=255)

                                                                                cgTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCCTGGTCAGTTCGTTTTCCGc         >  2:333763/1‑93 (MQ=255)

                                                                                cgTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGc         >  2:6318594/1‑93 (MQ=255)

                                                                                 gTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGcc        >  2:5491824/1‑93 (MQ=255)

                                                                                  tCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGcca       >  2:5623408/1‑93 (MQ=255)

                                                                                  tCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGcca       >  2:5873068/1‑93 (MQ=255)

                                                                                     ggACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGccacca    >  2:6197045/1‑93 (MQ=255)

                                                                                      gACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCcaccac   >  2:3648554/1‑93 (MQ=255)

                                                                                       aCCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACg  >  1:460533/1‑93 (MQ=255)

                                                                                       aCCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACg  >  1:103010/1‑93 (MQ=255)

ACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACG  >  NC_000913/3509108‑3509287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCC  >  NC_000913/3509105‑3509291

CCTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACTGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCG                                                                                               <  SRR4302534.2393581/94‑1 (MQ=60)

 CTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGC                                                                                              >  SRR4302534.6126975/1‑94 (MQ=60)

 CTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGC                                                                                              <  SRR4302534.4914983/94‑1 (MQ=60)

  TACCCGTTTTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCG                                                                                             <  SRR4302534.901308/94‑1 (MQ=60)

  TACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCG                                                                                             <  SRR4302534.3073048/94‑1 (MQ=60)

  TACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCG                                                                                             <  SRR4302534.350335/94‑1 (MQ=60)

   ACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGG                                                                                            <  SRR4302534.4663509/94‑1 (MQ=60)

   ACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGG                                                                                            >  SRR4302534.5607567/1‑94 (MQ=60)

   ACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGG                                                                                            >  SRR4302534.2254536/1‑94 (MQ=60)

     CCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTTTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCT                                                                                          >  SRR4302534.5464870/1‑94 (MQ=60)

     CCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCT                                                                                          >  SRR4302534.1582946/1‑94 (MQ=60)

     CCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCT                                                                                          <  SRR4302534.5705116/94‑1 (MQ=60)

      CGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTT                                                                                         <  SRR4302534.5773773/94‑1 (MQ=60)

      CGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTT                                                                                         <  SRR4302534.1514606/94‑1 (MQ=60)

      CGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTT                                                                                         >  SRR4302534.1756969/1‑94 (MQ=60)

       gtgggaGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTC                                                                                        <  SRR4302534.5489485/88‑1 (MQ=60)

       GTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTC                                                                                        <  SRR4302534.6597991/94‑1 (MQ=60)

       GTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTC                                                                                        <  SRR4302534.5913899/94‑1 (MQ=60)

       GTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTC                                                                                        >  SRR4302534.2062674/1‑94 (MQ=60)

       GTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTC                                                                                        <  SRR4302534.4577085/94‑1 (MQ=60)

        TTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCG                                                                                       <  SRR4302534.3095177/94‑1 (MQ=60)

        TTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCG                                                                                       <  SRR4302534.5115327/94‑1 (MQ=60)

         TGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGG                                                                                      >  SRR4302534.1073760/1‑94 (MQ=60)

          GTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGT                                                                                     <  SRR4302534.3351550/94‑1 (MQ=60)

           TTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTG                                                                                    <  SRR4302534.5907214/94‑1 (MQ=60)

           TTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTG                                                                                    <  SRR4302534.5442949/94‑1 (MQ=60)

             GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGC                                                                                  >  SRR4302534.2107170/1‑94 (MQ=60)

             GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGC                                                                                  <  SRR4302534.3349058/94‑1 (MQ=60)

               TGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCT                                                                                >  SRR4302534.4306336/1‑94 (MQ=60)

               TGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCT                                                                                <  SRR4302534.3328551/94‑1 (MQ=60)

                GGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTG                                                                               <  SRR4302534.1149900/94‑1 (MQ=60)

                 GGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGT                                                                              <  SRR4302534.546930/94‑1 (MQ=60)

                  GCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTA                                                                             >  SRR4302534.533420/1‑94 (MQ=60)

                       CAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAAT                                                                        >  SRR4302534.6592130/1‑94 (MQ=60)

                       CAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAAT                                                                        >  SRR4302534.3407771/1‑94 (MQ=60)

                         GGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCG                                                                      <  SRR4302534.2120893/94‑1 (MQ=60)

                           GCGTGTTTATCTACAACGGCACGCATCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCG                                                                    <  SRR4302534.5664224/94‑1 (MQ=60)

                           GCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCTGCTTCGGTGGCCTGTAAAAATCGCG                                                                    <  SRR4302534.3192433/94‑1 (MQ=60)

                            CGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGG                                                                   >  SRR4302534.5621992/1‑94 (MQ=60)

                            CGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGG                                                                   <  SRR4302534.2397020/94‑1 (MQ=60)

                            CGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGG                                                                   >  SRR4302534.5917871/1‑94 (MQ=60)

                              TGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGAT                                                                 <  SRR4302534.4961282/94‑1 (MQ=60)

                              TGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGAT                                                                 <  SRR4302534.61924/94‑1 (MQ=60)

                               GTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATC                                                                <  SRR4302534.6340268/94‑1 (MQ=60)

                               GTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATC                                                                <  SRR4302534.1504546/94‑1 (MQ=60)

                                 TTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGT                                                              <  SRR4302534.2742738/94‑1 (MQ=60)

                                  TATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCCACTCGCGTCAGGACCGCGGCTGCGGTGGCCTGTAAAAATCGCGGATCGTC                                                             <  SRR4302534.1866133/94‑1 (MQ=60)

                                   ATCTACACCGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCT                                                            <  SRR4302534.2455463/94‑1 (MQ=60)

                                   ATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCT                                                            <  SRR4302534.4600078/94‑1 (MQ=60)

                                    TCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTG                                                           <  SRR4302534.2815092/94‑1 (MQ=60)

                                    TCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTG                                                           <  SRR4302534.5940221/94‑1 (MQ=60)

                                     CTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGT                                                          <  SRR4302534.5264826/94‑1 (MQ=60)

                                      TACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTG                                                         >  SRR4302534.3871166/1‑94 (MQ=60)

                                      TACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTG                                                         <  SRR4302534.6328933/94‑1 (MQ=60)

                                       GCAACGTCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACGGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGA                                                        <  SRR4302534.3730300/94‑1 (MQ=60)

                                       ACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGA                                                        >  SRR4302534.4564994/1‑94 (MQ=60)

                                        CAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAG                                                       <  SRR4302534.2712330/94‑1 (MQ=60)

                                        CAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAG                                                       >  SRR4302534.2479671/1‑94 (MQ=60)

                                        CAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAG                                                       >  SRR4302534.3238537/1‑94 (MQ=60)

                                         AACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGC                                                      >  SRR4302534.1034527/1‑94 (MQ=60)

                                         AACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGC                                                      <  SRR4302534.1413184/94‑1 (MQ=60)

                                          ACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCG                                                     >  SRR4302534.3200078/1‑94 (MQ=60)

                                          ACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCG                                                     <  SRR4302534.2662487/94‑1 (MQ=60)

                                          ACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCG                                                     >  SRR4302534.10597/1‑94 (MQ=60)

                                           CGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGG                                                    <  SRR4302534.420590/94‑1 (MQ=60)

                                           CGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGG                                                    <  SRR4302534.3057020/94‑1 (MQ=60)

                                            GGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGC                                                   <  SRR4302534.5750052/94‑1 (MQ=60)

                                              CACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGT                                                 >  SRR4302534.4440153/1‑94 (MQ=60)

                                              CACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGT                                                 >  SRR4302534.1034328/1‑94 (MQ=60)

                                              CACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGT                                                 >  SRR4302534.4468153/1‑94 (MQ=60)

                                               CCGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTC                                                <  SRR4302534.1609806/94‑1 (MQ=60)

                                               ACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTC                                                <  SRR4302534.6281987/94‑1 (MQ=60)

                                                CGCAGCAGTTGTTGCATTGGGTAATCGTCGACTCGCGTCAGGCCCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCG                                               <  SRR4302534.5342233/94‑1 (MQ=60)

                                                CGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGGGGATCagctgggcgcggggtcg                                               >  SRR4302534.1655810/1‑77 (MQ=60)

                                                CGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCG                                               <  SRR4302534.2767079/94‑1 (MQ=60)

                                                CGCAGCAGTTGTTGCATTGGGGAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGGGGGCGGCGTCG                                               >  SRR4302534.66511/1‑94 (MQ=60)

                                                 GCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGC                                              <  SRR4302534.4415687/94‑1 (MQ=60)

                                                  CAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCC                                             >  SRR4302534.6118247/1‑94 (MQ=60)

                                                   AGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGTCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCT                                            <  SRR4302534.1482436/94‑1 (MQ=60)

                                                   AGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCT                                            <  SRR4302534.4869043/94‑1 (MQ=60)

                                                   AGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCT                                            >  SRR4302534.1717303/1‑94 (MQ=60)

                                                   AGCAGTTGTTGCATTGGATAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCT                                            <  SRR4302534.5139166/94‑1 (MQ=60)

                                                     CAGTTGTTGCATTGGTTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                          <  SRR4302534.5838392/94‑1 (MQ=60)

                                                     CAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                          <  SRR4302534.4271022/94‑1 (MQ=60)

                                                     CAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                          <  SRR4302534.631944/94‑1 (MQ=60)

                                                     CAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCT                                          <  SRR4302534.3795697/94‑1 (MQ=60)

                                                      AGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTA                                         >  SRR4302534.1427775/1‑94 (MQ=60)

                                                      AGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTA                                         <  SRR4302534.1146067/94‑1 (MQ=60)

                                                      AGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTA                                         <  SRR4302534.3032653/94‑1 (MQ=60)

                                                      AGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGCCTGTGAGCGGGGTTGCCTCCA                                         >  SRR4302534.3201765/1‑94 (MQ=60)

                                                       GTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAA                                        >  SRR4302534.2720293/1‑94 (MQ=60)

                                                       GTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAA                                        >  SRR4302534.2192191/1‑94 (MQ=60)

                                                        TTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAAT                                       <  SRR4302534.1981052/94‑1 (MQ=60)

                                                          TTTGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGTCCTGTAAAAATCGCGGATCGTCTGTGAGCTGCGTCGCCTCTAATCC                                     <  SRR4302534.3265869/94‑1 (MQ=60)

                                                            TGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTTATCCCA                                   >  SRR4302534.5737679/1‑94 (MQ=60)

                                                            TGCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCA                                   <  SRR4302534.502344/94‑1 (MQ=60)

                                                             GCATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAG                                  >  SRR4302534.2196932/1‑94 (MQ=60)

                                                               ATTGGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCA                                >  SRR4302534.2521811/1‑94 (MQ=60)

                                                               ATTGGGGAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCA                                >  SRR4302534.2902310/1‑94 (MQ=60)

                                                                  GGGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGG                             <  SRR4302534.4659747/94‑1 (MQ=60)

                                                                   gtgAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGGCTCTAATCCCAGCATGGT                            >  SRR4302534.6112884/4‑94 (MQ=60)

                                                                   GGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGGCTCTAATCCCAGCATGGT                            >  SRR4302534.3011609/1‑94 (MQ=60)

                                                                   GGTAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGT                            <  SRR4302534.1393527/94‑1 (MQ=60)

                                                                     TAATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGCCCTGTAAAAACCGCGTATCGGCTGTGAGAGGCGTCGCCTCTACTCACAGCATGGTCA                          <  SRR4302534.4619827/94‑1 (MQ=60)

                                                                       ATCATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGT                        <  SRR4302534.4365603/94‑1 (MQ=60)

                                                                         CATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTC                      <  SRR4302534.2392555/94‑1 (MQ=60)

                                                                         CATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTC                      >  SRR4302534.534787/1‑94 (MQ=60)

                                                                         CATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGGCTGTGAGCGGGGTCGCCTCTAATCCCAGCATGGTCAGTTC                      >  SRR4302534.365201/1‑94 (MQ=60)

                                                                          ATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATggcgaaccgcctggaagcggcgcgccctctaatcccggcagggcagtttgt                     >  SRR4302534.572303/1‑43 (MQ=60)

                                                                          ATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCG                     <  SRR4302534.2572826/94‑1 (MQ=60)

                                                                          ATCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATAGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCG                     >  SRR4302534.2892305/1‑94 (MQ=60)

                                                                           TCGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGT                    >  SRR4302534.882930/1‑94 (MQ=60)

                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                   >  SRR4302534.984125/1‑94 (MQ=60)

                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                   >  SRR4302534.5369973/1‑94 (MQ=60)

                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                   >  SRR4302534.1406659/1‑94 (MQ=60)

                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                   >  SRR4302534.4752720/1‑94 (MQ=60)

                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                   <  SRR4302534.397056/94‑1 (MQ=60)

                                                                            CGACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                   >  SRR4302534.4023360/1‑94 (MQ=60)

                                                                            CGACTCGCCTCAGGCCCGCGGCTCCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTT                   <  SRR4302534.4629430/94‑1 (MQ=60)

                                                                             GACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTT                  <  SRR4302534.1457926/94‑1 (MQ=60)

                                                                              GCTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTT                 <  SRR4302534.4108458/94‑1 (MQ=60)

                                                                              ACTCGCGTCAGGACCGCGGCTTGGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTT                 <  SRR4302534.5626210/94‑1 (MQ=60)

                                                                              ACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTT                 >  SRR4302534.967977/1‑94 (MQ=60)

                                                                              ACTCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTT                 <  SRR4302534.1924959/94‑1 (MQ=60)

                                                                                TCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCC               <  SRR4302534.3771439/94‑1 (MQ=60)

                                                                                TCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCC               <  SRR4302534.3794007/94‑1 (MQ=60)

                                                                                TCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCC               <  SRR4302534.2566591/94‑1 (MQ=60)

                                                                                TCGCGTCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCC               <  SRR4302534.4729850/94‑1 (MQ=60)

                                                                                    GGCAGGACCGCGGCTTCGCTGGCCGGTAAAAATCGCGGATCGTCGGTGAGCGGCGCCGCCTCGAAACCCAGAATGG‑CAGTTCTTTTTCCGCCAC          <  SRR4302534.2197827/94‑1 (MQ=34)

                                                                                     TCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCAC          >  SRR4302534.5232179/1‑94 (MQ=60)

                                                                                     TCAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGATGCGTAGCCTCTAATCCCATCATGGTCAGTTCGTTTTTCGCCAC          >  SRR4302534.6506853/1‑94 (MQ=60)

                                                                                      CAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACC         >  SRR4302534.5855609/1‑94 (MQ=60)

                                                                                      CAGGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACC         >  SRR4302534.3038328/1‑94 (MQ=60)

                                                                                        GGACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCAC       <  SRR4302534.2738649/94‑1 (MQ=60)

                                                                                         GACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACG      >  SRR4302534.105760/1‑94 (MQ=60)

                                                                                         GACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACG      >  SRR4302534.472164/1‑94 (MQ=60)

                                                                                          ACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGT     <  SRR4302534.3800089/94‑1 (MQ=60)

                                                                                          ACCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGT     >  SRR4302534.1653225/1‑94 (MQ=60)

                                                                                           CCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTT    >  SRR4302534.3203572/1‑94 (MQ=60)

                                                                                           CCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTT    <  SRR4302534.3906606/94‑1 (MQ=60)

                                                                                           CCGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTT    <  SRR4302534.2722818/94‑1 (MQ=60)

                                                                                            CGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC   <  SRR4302534.4051806/94‑1 (MQ=60)

                                                                                            CGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC   <  SRR4302534.4051807/94‑1 (MQ=60)

                                                                                            CGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC   <  SRR4302534.5036424/94‑1 (MQ=60)

                                                                                            CGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC   <  SRR4302534.5192178/94‑1 (MQ=60)

                                                                                            CGCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC   <  SRR4302534.52784/94‑1 (MQ=60)

                                                                                            CGCGGCTCCGGTGGCCTGTAAAAGTCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTC   <  SRR4302534.4916036/94‑1 (MQ=60)

                                                                                             GCGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCC  >  SRR4302534.4459548/1‑94 (MQ=60)

ACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCC  >  NC_000913/3509105‑3509291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`