Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,573,700	+C	intergenic (+403/+997)	yhgN → / ← gntK	hypothetical protein/gluconokinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,573,700	1	.	C	99.5%	707.7 / NA	209	intergenic (+403/+997)	yhgN/gntK	hypothetical protein/gluconokinase 2
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (104/104); minor base A (1/0); total (105/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 238 total aligned reads displayed.

AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786

aaCAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACg                                                                                         <  2:6216185/93‑1 (MQ=255)

aaCAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACg                                                                                         >  2:2199724/1‑93 (MQ=255)

aaCAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGAGATAGCAATCGGCAGGCCCATGCCGGTTAACg                                                                                         >  1:768285/1‑93 (MQ=255)

  cAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgct                                                                                       <  2:2083294/93‑3 (MQ=255)

    ggCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgcttc                                                                                     >  2:1506017/1‑89 (MQ=255)

    ggCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgcttc                                                                                     >  1:2133848/1‑89 (MQ=255)

    ggCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgcttc                                                                                     <  2:1080984/93‑5 (MQ=255)

    ggCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgcttc                                                                                     <  1:835008/93‑5 (MQ=255)

      cTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGc                                                                                   >  2:783200/1‑93 (MQ=255)

        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTTCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGccc                                                                                 <  2:1444291/93‑1 (MQ=255)

        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGccc                                                                                 >  2:934133/1‑93 (MQ=255)

        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGccc                                                                                 >  1:247758/1‑93 (MQ=255)

          ggTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAg                                                                               >  2:4367703/1‑93 (MQ=255)

           gTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGt                                                                              <  1:1100095/93‑1 (MQ=255)

           gTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGt                                                                              >  1:3071374/1‑93 (MQ=255)

            tGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTg                                                                             >  2:5670880/1‑93 (MQ=255)

             ggCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGCTAACGCTTCGCCCAGTGc                                                                            >  2:5009181/1‑93 (MQ=255)

              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGcc                                                                           >  1:4512815/1‑93 (MQ=255)

                aGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCgg                                                                         >  1:5195339/1‑93 (MQ=255)

                  aaCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAc                                                                       <  2:2447152/93‑1 (MQ=255)

                   aCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAcc                                                                      >  2:3886421/1‑93 (MQ=255)

                     ccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTa                                                                    >  1:2197365/1‑93 (MQ=255)

                     ccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTa                                                                    >  2:5261911/1‑93 (MQ=255)

                      cTGAATGATGCGGCCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAACCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAc                                                                   <  2:3574861/93‑1 (MQ=255)

                       tGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGGCCTCCg                                                                  >  2:468038/1‑93 (MQ=255)

                       tGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACg                                                                  >  1:3399154/1‑93 (MQ=255)

                       tGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACg                                                                  >  2:4610310/1‑93 (MQ=255)

                         aaTGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGcc                                                                <  1:6011857/93‑1 (MQ=255)

                          atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCa                                                               >  1:1410438/1‑93 (MQ=255)

                          atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCa                                                               >  1:1365920/1‑93 (MQ=255)

                           tgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCag                                                              >  1:4844242/1‑93 (MQ=255)

                            gatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCaga                                                             <  1:376199/93‑1 (MQ=255)

                            gatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCaga                                                             >  1:5731571/1‑93 (MQ=255)

                             atgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCagag                                                            <  2:6018695/93‑1 (MQ=255)

                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           <  2:1817305/93‑1 (MQ=255)

                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           >  2:373524/1‑93 (MQ=255)

                                 gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTGGCCCAGTGCCGGACCTACGCCAGAGGCac                                                        >  2:4319785/1‑92 (MQ=255)

                                 gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCaa                                                        >  2:273767/1‑93 (MQ=255)

                                 gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCaa                                                        >  2:6127238/1‑93 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      >  2:327157/1‑93 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      <  1:3493670/93‑1 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      >  1:5313908/1‑93 (MQ=255)

                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     <  1:5228947/93‑1 (MQ=255)

                                     tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAg                                                    >  1:253750/1‑93 (MQ=255)

                                     tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAg                                                    <  2:639647/93‑1 (MQ=255)

                                     tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAg                                                    >  1:3415365/1‑93 (MQ=255)

                                     tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGACTCAACCAg                                                    <  1:6296334/93‑1 (MQ=255)

                                      gCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGc                                                   <  1:4787310/93‑1 (MQ=255)

                                      gCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGc                                                   >  2:1925746/1‑93 (MQ=255)

                                       cAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCa                                                  <  1:32653/93‑1 (MQ=255)

                                         gCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAcc                                                <  1:314796/93‑1 (MQ=255)

                                         gCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAcc                                                >  2:470765/1‑93 (MQ=255)

                                          cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                                               <  2:3077304/93‑1 (MQ=255)

                                          cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                                               <  2:5394825/93‑1 (MQ=255)

                                             ccAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGtt                                            <  1:2356834/93‑1 (MQ=255)

                                             ccAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGtt                                            <  2:1047363/93‑1 (MQ=255)

                                               aGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTg                                          <  2:1217529/93‑1 (MQ=255)

                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:1062222/1‑93 (MQ=255)

                                                  aCGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                                       >  2:3470954/1‑93 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGGCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     >  2:3962603/1‑93 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGCacac                                     >  1:2880480/1‑93 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  2:5789974/93‑1 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  1:6335472/93‑1 (MQ=255)

                                                     aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcg                                    >  2:1527410/1‑93 (MQ=255)

                                                     aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcg                                    <  1:3687688/93‑1 (MQ=255)

                                                         aGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgccgc                                <  2:3703363/93‑1 (MQ=255)

                                                           gTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgccc                              <  2:3394923/93‑1 (MQ=255)

                                                               tAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAc                          <  1:1203500/93‑1 (MQ=255)

                                                                  cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCaa                       <  1:672058/93‑1 (MQ=255)

                                                                  cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCaa                       >  2:5069037/1‑93 (MQ=255)

                                                                  cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCaa                       <  1:4153497/93‑1 (MQ=255)

                                                                   aaTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAAt                      >  2:3033544/1‑93 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     >  2:4214029/1‑93 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     >  2:478010/1‑93 (MQ=255)

                                                                     tCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa                    >  1:5928061/1‑93 (MQ=255)

                                                                       ggCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAcc                  <  2:2500744/93‑1 (MQ=255)

                                                                       ggCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAcc                  >  2:2150302/1‑93 (MQ=255)

                                                                       ggCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAcc                  <  2:1312942/93‑1 (MQ=255)

                                                                        gCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACca                 <  1:1037222/93‑1 (MQ=255)

                                                                         cAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcag                <  1:2224190/93‑1 (MQ=255)

                                                                           ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagca              <  2:648846/93‑1 (MQ=255)

                                                                           ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagca              >  2:665604/1‑93 (MQ=255)

                                                                            gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagcag             >  1:918793/1‑93 (MQ=255)

                                                                            gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagcag             >  1:4491705/1‑93 (MQ=255)

                                                                             cccATGCCAGTTAACGCTTTGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGa            >  1:6265657/1‑93 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           >  2:4575933/1‑93 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           <  1:1995623/93‑1 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           >  1:4421239/1‑93 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           <  1:3047250/93‑1 (MQ=255)

                                                                               cATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAt          >  1:1484987/1‑93 (MQ=255)

                                                                               cATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAt          >  2:409825/1‑93 (MQ=255)

                                                                                 tGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGa        <  1:5502257/93‑1 (MQ=255)

                                                                                 tGCCGGTTAACGATTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCCATCACAAGCAGAATGa        >  1:4347912/1‑93 (MQ=255)

                                                                                  gCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAt       <  1:4596480/93‑1 (MQ=255)

                                                                                    cGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcc     <  2:4148893/93‑1 (MQ=255)

                                                                                     ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  2:4433563/93‑1 (MQ=255)

                                                                                      gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   <  2:547816/93‑1 (MQ=255)

                                                                                      gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   >  1:2417671/1‑93 (MQ=255)

                                                                                      gTTAACGCTTCGCCCAGTCCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   <  1:5390791/93‑1 (MQ=255)

                                                                                      gTTAACGCTTCGCCAAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   <  1:997920/93‑1 (MQ=255)

AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  NC_000913/3573607‑3573793

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGAGATAGCAATCGGCAGGCCCATGCCGGTTAACG                                                                                                >  SRR4302534.787382/1‑94 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            >  SRR4302534.2190077/1‑94 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            <  SRR4302534.3142236/94‑1 (MQ=60)

     GGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCG                                                                                           <  SRR4302534.64691/94‑1 (MQ=60)

     GGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCG                                                                                           <  SRR4302534.856029/94‑1 (MQ=60)

     GGCTACGGTGGCAGAACCATGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGCCAGGCCCATGCCGGTTAACGCTTCG                                                                                           <  SRR4302534.4490913/94‑1 (MQ=60)

      GCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGC                                                                                          <  SRR4302534.309988/94‑1 (MQ=60)

      GCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGC                                                                                          <  SRR4302534.6429187/94‑1 (MQ=60)

        TACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCC                                                                                        >  SRR4302534.254229/1‑94 (MQ=60)

           GGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGT                                                                                     >  SRR4302534.6258459/1‑94 (MQ=60)

           GGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGT                                                                                     >  SRR4302534.3152062/1‑94 (MQ=60)

           GGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGT                                                                                     <  SRR4302534.2236256/94‑1 (MQ=60)

            GTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTG                                                                                    <  SRR4302534.1128760/94‑1 (MQ=60)

             TGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGC                                                                                   >  SRR4302534.3099419/1‑94 (MQ=60)

              GGCGGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCC                                                                                  <  SRR4302534.6062232/94‑1 (MQ=60)

              GGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCC                                                                                  <  SRR4302534.3688186/94‑1 (MQ=60)

              GGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCC                                                                                  >  SRR4302534.4635041/1‑94 (MQ=60)

               GCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCCCGAAGCAGGTGATAGCAATCGGCAGGCCCCTGCCGGTTAACGCTTCGCCCAGTGCCG                                                                                 >  SRR4302534.2230130/1‑94 (MQ=60)

               GCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATAGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCG                                                                                 >  SRR4302534.25917/1‑94 (MQ=60)

                CAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGG                                                                                >  SRR4302534.5335440/1‑94 (MQ=60)

                 AGAACCCTGAATGTTGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAAAGCTTCGCCCAGTGCTGGA                                                                               >  SRR4302534.877097/1‑94 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGGCGGTTAACGCTTCGCCCAGTGCCGGA                                                                               >  SRR4302534.5343205/1‑94 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGgcgca                                                                               >  SRR4302534.685035/1‑89 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGA                                                                               >  SRR4302534.323367/1‑94 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGA                                                                               >  SRR4302534.814393/1‑94 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCCGTTAAAGCTTCGCCCAGTGGCGGA                                                                               >  SRR4302534.1348351/1‑94 (MQ=60)

                  GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAC                                                                              >  SRR4302534.4563932/1‑94 (MQ=60)

                   AACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACC                                                                             >  SRR4302534.524483/1‑94 (MQ=60)

                    ACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCT                                                                            <  SRR4302534.4956287/94‑1 (MQ=60)

                     CCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTA                                                                           >  SRR4302534.2255507/1‑94 (MQ=60)

                      CCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAC                                                                          <  SRR4302534.6379571/94‑1 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         >  SRR4302534.5291800/1‑94 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         >  SRR4302534.3490392/1‑94 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         <  SRR4302534.1185073/94‑1 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         >  SRR4302534.4693331/1‑94 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         <  SRR4302534.5861748/94‑1 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      <  SRR4302534.6175208/94‑1 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      >  SRR4302534.1401892/1‑94 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      >  SRR4302534.1447543/1‑94 (MQ=60)

                           ATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAG                                                                     <  SRR4302534.5521882/94‑1 (MQ=60)

                           ATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAG                                                                     >  SRR4302534.4975678/1‑94 (MQ=60)

                            TGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGA                                                                    <  SRR4302534.349306/94‑1 (MQ=60)

                            TGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGA                                                                    >  SRR4302534.5886882/1‑94 (MQ=60)

                             GATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAG                                                                   <  SRR4302534.385812/94‑1 (MQ=60)

                              GTGCGCACTGCAGCTGCCAGAACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGT                                                                  <  SRR4302534.3738622/94‑1 (MQ=60)

                                GCGCAGTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCA                                                                <  SRR4302534.6607803/94‑1 (MQ=60)

                                GCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCA                                                                <  SRR4302534.3402161/94‑1 (MQ=60)

                                   CACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCCACC                                                             >  SRR4302534.34829/1‑94 (MQ=60)

                                   CACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACC                                                             >  SRR4302534.5457270/1‑94 (MQ=60)

                                    ACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCA                                                            <  SRR4302534.2469038/94‑1 (MQ=60)

                                    ACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCA                                                            <  SRR4302534.3587593/94‑1 (MQ=60)

                                    ACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCA                                                            >  SRR4302534.1064972/1‑94 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           >  SRR4302534.260367/1‑94 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           >  SRR4302534.3507122/1‑94 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           <  SRR4302534.5370114/94‑1 (MQ=60)

                                      TGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGACTCAACCAGC                                                          <  SRR4302534.6466631/94‑1 (MQ=60)

                                       GCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATTACGGGTAACGCTTCGCTCAGTGTCGGACCTACGCCAGAGTCGACCAGCA                                                         >  SRR4302534.5500802/1‑94 (MQ=60)

                                       GCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCA                                                         <  SRR4302534.4917311/94‑1 (MQ=60)

                                        CAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAC                                                        <  SRR4302534.1650898/94‑1 (MQ=60)

                                        CAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAC                                                        <  SRR4302534.33507/94‑1 (MQ=60)

                                         AGCAGCCAGCACGACGCAGGGGATAGCAATAGGCAGGTCCCTGCCGGTTAACGACTCGCACAGTGCCGGACCTACGCCAGAGACAACCAGCCCC                                                       >  SRR4302534.3852231/1‑94 (MQ=32)

                                          GCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCT                                                      <  SRR4302534.322907/94‑1 (MQ=60)

                                           CTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTG                                                     <  SRR4302534.3193458/94‑1 (MQ=60)

                                           CTGCAAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTG                                                     <  SRR4302534.1239371/94‑1 (MQ=60)

                                             GCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTT                                                   <  SRR4302534.2500308/94‑1 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAGCTACGCCAGAGTCAACCAGCACCTGATT                                                  >  SRR4302534.2703170/1‑94 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  <  SRR4302534.471555/94‑1 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  <  SRR4302534.2419379/94‑1 (MQ=60)

                                               CAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTG                                                 >  SRR4302534.314217/1‑94 (MQ=60)

                                                AGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGA                                                >  SRR4302534.4550030/1‑94 (MQ=60)

                                                  CACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGGCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAAC                                              >  SRR4302534.4031942/1‑94 (MQ=60)

                                                   ACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACA                                             >  SRR4302534.809933/1‑94 (MQ=60)

                                                   ACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACA                                             <  SRR4302534.2146430/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGCACAC                                            >  SRR4302534.2956079/1‑94 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGCCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302534.5512085/94‑1 (MQ=60)

                                                     GAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACG                                           <  SRR4302534.6506619/94‑1 (MQ=60)

                                                     GAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACG                                           >  SRR4302534.6092611/1‑94 (MQ=60)

                                                      AAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGC                                          <  SRR4302534.3786842/94‑1 (MQ=60)

                                                      AAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGATCACGC                                          >  SRR4302534.1846054/1‑94 (MQ=60)

                                                         CAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGC                                       >  SRR4302534.6460216/1‑94 (MQ=60)

                                                          AGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGGCGGACCTACGCCAGAGACAACCAGCACCTGATTGAACACGCCGTC                                      >  SRR4302534.3837262/1‑94 (MQ=60)

                                                           GGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCC                                     >  SRR4302534.5538204/1‑94 (MQ=60)

                                                           GGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCC                                     <  SRR4302534.690379/94‑1 (MQ=60)

                                                            GTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCG                                    <  SRR4302534.24522/94‑1 (MQ=60)

                                                              GATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCA                                  <  SRR4302534.5381894/94‑1 (MQ=60)

                                                                TAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACC                                <  SRR4302534.1235168/94‑1 (MQ=60)

                                                                   CAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAAT                             <  SRR4302534.688446/94‑1 (MQ=60)

                                                                   CAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAAT                             <  SRR4302534.4265629/94‑1 (MQ=60)

                                                                    AATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATC                            >  SRR4302534.2303333/1‑94 (MQ=60)

                                                                     ATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCA                           >  SRR4302534.6089044/1‑94 (MQ=60)

                                                                      TCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAC                          <  SRR4302534.5657824/94‑1 (MQ=60)

                                                                         GCAGTCCAATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAG                       <  SRR4302534.1417738/94‑1 (MQ=60)

                                                                         GCAGGGCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTGAAAAAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAG                       >  SRR4302534.5892400/1‑94 (MQ=60)

                                                                         GCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAG                       <  SRR4302534.1063991/94‑1 (MQ=60)

                                                                          CAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGC                      <  SRR4302534.2283092/94‑1 (MQ=60)

                                                                          CAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGC                      <  SRR4302534.2948862/94‑1 (MQ=60)

                                                                           AGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCA                     <  SRR4302534.4070839/94‑1 (MQ=60)

                                                                            GGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAG                    <  SRR4302534.991552/94‑1 (MQ=60)

                                                                            GGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAG                    >  SRR4302534.942191/1‑94 (MQ=60)

                                                                            GGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAG                    >  SRR4302534.4613244/1‑94 (MQ=60)

                                                                             GCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGA                   <  SRR4302534.4662383/94‑1 (MQ=60)

                                                                             GCCCATGCCAGTTAACGCTTTGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGA                   >  SRR4302534.6435354/1‑94 (MQ=60)

                                                                              CCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAA                  >  SRR4302534.4540600/1‑94 (MQ=60)

                                                                              CCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAA                  <  SRR4302534.4624989/94‑1 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 >  SRR4302534.1524059/1‑94 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 <  SRR4302534.3127187/94‑1 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 <  SRR4302534.2047890/94‑1 (MQ=60)

                                                                                 ATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGA               <  SRR4302534.358136/94‑1 (MQ=60)

                                                                                 ATGCCGGTTAACGATTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCCATCACAAGCAGAATGA               >  SRR4302534.4465307/1‑94 (MQ=60)

                                                                                  TGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAT              <  SRR4302534.5650654/94‑1 (MQ=60)

                                                                                   GCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCCGCACCTGTTTGAACACGCCGACCGCACCAATCACCAGCAGAATGATC             >  SRR4302534.6030738/1‑94 (MQ=60)

                                                                                   GCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATC             <  SRR4302534.4721227/94‑1 (MQ=60)

                                                                                    CCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCC            >  SRR4302534.437031/1‑94 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          >  SRR4302534.2481970/1‑94 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302534.151738/94‑1 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302534.170138/94‑1 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTCCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302534.5535956/94‑1 (MQ=60)

                                                                                       GTTAACGCTTCGCCAAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302534.1023493/94‑1 (MQ=60)

                                                                                        TTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCCCCAGCAGAATGATCCCCGC        <  SRR4302534.2274462/94‑1 (MQ=60)

                                                                                         TAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCC       <  SRR4302534.4770357/94‑1 (MQ=60)

                                                                                          AACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCG      >  SRR4302534.1462992/1‑94 (MQ=60)

                                                                                          AACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCG      >  SRR4302534.4226696/1‑94 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  <  SRR4302534.2837841/94‑1 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  <  SRR4302534.3721973/94‑1 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  SRR4302534.3785399/1‑94 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGAACCAATCACCAGCAGAATGATCCCCGCCGGCTG  >  SRR4302534.3230872/1‑94 (MQ=60)

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  NC_000913/3573607‑3573793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`