Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,951	T→C	V372A (GTG→GCG)	recG →	DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,951	0	T	C	99.7%	995.1 / NA	286	V372A (GTG→GCG)	recG	DNA helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (124/161); minor base G (1/0); total (125/161)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

CACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTT  >  NC_000913/3823861‑3824040

 aCCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCa                                                                                        >  2:145142/1‑93 (MQ=255)

 aCCGCTCGGTATCGAAGTGGGCCGGCTCGCCGATAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCa                                                                                        <  2:4202751/93‑1 (MQ=255)

  ccGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAg                                                                                       >  2:2794693/1‑93 (MQ=255)

   cGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAga                                                                                      <  2:1252074/93‑1 (MQ=255)

    gCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgat                                                                                     >  1:1099188/1‑93 (MQ=255)

    gCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgat                                                                                     <  2:2187420/93‑1 (MQ=255)

    gCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgat                                                                                     <  1:5433175/93‑1 (MQ=255)

    gCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgat                                                                                     <  1:3611292/93‑1 (MQ=255)

    gCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgat                                                                                     >  2:6401335/1‑93 (MQ=255)

     cTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgctg                                                                                    >  1:1867479/1‑93 (MQ=255)

     cTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgatg                                                                                    <  1:4066297/93‑1 (MQ=255)

      tCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgatga                                                                                   <  1:2861457/93‑1 (MQ=255)

       cGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAgatgat                                                                                  >  1:6316481/1‑93 (MQ=255)

        ggTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGAtt                                                                                 <  2:3787240/93‑1 (MQ=255)

        ggTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGAtt                                                                                 <  1:441849/93‑1 (MQ=255)

        ggTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGAtt                                                                                 <  1:4209985/93‑1 (MQ=255)

         gTATCGAAGTGGGCTGGCGCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTg                                                                                <  1:1599001/93‑1 (MQ=255)

          tATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGt                                                                               <  1:3237013/93‑1 (MQ=255)

          tATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGt                                                                               >  1:1329030/1‑93 (MQ=255)

           aTCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGGGGGCAGGCGCAGATGATTGtg                                                                              >  2:5356647/1‑92 (MQ=255)

           aTCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTc                                                                              <  2:555978/93‑1 (MQ=255)

           aTCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTc                                                                              <  2:5063578/93‑1 (MQ=255)

           aTCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTCGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTc                                                                              <  2:4173591/93‑1 (MQ=255)

              gAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGt                                                                           <  2:4326901/93‑1 (MQ=255)

              gAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGt                                                                           >  2:5391524/1‑93 (MQ=255)

              gAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGt                                                                           >  1:2360296/1‑93 (MQ=255)

               aaGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTa                                                                          <  2:200651/93‑1 (MQ=255)

               aaGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTa                                                                          >  2:1006415/1‑93 (MQ=255)

                aGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTac                                                                         <  1:6377931/93‑1 (MQ=255)

                 gTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCCCGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTAGGTacc                                                                        >  2:4254498/1‑92 (MQ=255)

                 gTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTaca                                                                        >  2:685383/1‑93 (MQ=255)

                  tGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTacac                                                                       <  1:6185672/93‑1 (MQ=255)

                  ggggCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTacac                                                                       <  2:775040/92‑1 (MQ=255)

                    ggctggctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTacacac                                                                     <  2:4029417/93‑1 (MQ=255)

                    ggctggctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTacacac                                                                     <  2:3330161/93‑1 (MQ=255)

                     gctggctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACg                                                                    >  1:5872115/1‑93 (MQ=255)

                      ctggctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGc                                                                   <  2:350581/93‑1 (MQ=255)

                      ctggctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGc                                                                   <  1:116011/93‑1 (MQ=255)

                      ctggctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGc                                                                   <  2:2704069/93‑1 (MQ=255)

                        ggctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCa                                                                 <  2:2069183/93‑1 (MQ=255)

                         gctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCAt                                                                >  1:4899106/1‑93 (MQ=255)

                         gctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCAt                                                                <  1:5622712/93‑1 (MQ=255)

                         gctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCAt                                                                <  1:6115515/93‑1 (MQ=255)

                          ctCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATc                                                               <  2:6183339/93‑1 (MQ=255)

                            cGCCGGTAATCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCtt                                                             <  1:849062/93‑1 (MQ=255)

                             gCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTc                                                            >  1:3858701/1‑93 (MQ=255)

                               cGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCCTCCa                                                          >  1:1006416/1‑93 (MQ=255)

                                ggTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAg                                                         <  1:4238866/93‑1 (MQ=255)

                                ggTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAg                                                         <  2:4822045/93‑1 (MQ=255)

                                  tAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGa                                                       >  2:3653818/1‑93 (MQ=255)

                                  tAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGa                                                       <  2:188103/93‑1 (MQ=255)

                                    aGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAAc                                                     >  2:857018/1‑93 (MQ=255)

                                    aGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAAc                                                     <  1:4695047/93‑1 (MQ=255)

                                      cAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAg                                                   <  1:780822/93‑1 (MQ=255)

                                       aGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAgg                                                  >  2:4596216/1‑93 (MQ=255)

                                       aGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAgg                                                  >  1:298489/1‑93 (MQ=255)

                                        gAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAggg                                                 >  2:3758609/1‑92 (MQ=255)

                                        gAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGt                                                 >  1:3415308/1‑93 (MQ=255)

                                          aaGGTAAAGCACGGCTGACACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGc                                               <  1:5545454/93‑1 (MQ=255)

                                           aGGTAAAGCACGGCTGGCACAGCAGGAATCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCa                                              <  2:2588806/93‑1 (MQ=255)

                                            ggTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGCGCAg                                             >  1:2196606/1‑93 (MQ=255)

                                              tAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGtt                                           <  1:2250953/93‑1 (MQ=255)

                                                  gCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAAc                                       <  2:6257540/93‑1 (MQ=255)

                                                  gCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAAc                                       <  2:398166/93‑1 (MQ=255)

                                                      ggCTGGCACAGCAGGAAGCCATCGCCAGGGGTCAGGGGCAGATGATTGTCGGTACACACGCCACCTTCCAGGAACAGGTGCAGTTTAACGGcc                                   >  1:1692304/1‑93 (MQ=255)

                                                      ggCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGcc                                   <  2:6085159/93‑1 (MQ=255)

                                                      ggCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGcc                                   <  2:3861158/93‑1 (MQ=255)

                                                       gCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCt                                  >  2:5182910/1‑93 (MQ=255)

                                                       gCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGAGCAGGTTAACGGCCt                                  >  1:2427001/1‑93 (MQ=255)

                                                            cacaGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGgcgc                             <  2:467853/93‑1 (MQ=255)

                                                             acaGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCt                            >  1:3557235/1‑93 (MQ=255)

                                                              cagcagGAAGGCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTg                           <  1:5735145/93‑1 (MQ=255)

                                                                gcagTAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGt                         <  1:4763813/93‑1 (MQ=255)

                                                                gcagGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGt                         <  1:2141041/93‑1 (MQ=255)

                                                                  agGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGa                       >  2:4537524/1‑93 (MQ=255)

                                                                  agGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGa                       <  1:2701782/93‑1 (MQ=255)

                                                                   ggAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGAt                      <  2:2626338/93‑1 (MQ=255)

                                                                      aGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTAt                   >  2:4952789/1‑93 (MQ=255)

                                                                      aGCAATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTAt                   <  2:602797/93‑1 (MQ=255)

                                                                       gCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATc                  <  1:3664539/93‑1 (MQ=255)

                                                                       gCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATc                  >  2:4358577/1‑93 (MQ=255)

                                                                        ccATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcg                 <  2:3054387/93‑1 (MQ=255)

                                                                         cATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcga                <  2:22104/93‑1 (MQ=255)

                                                                          aTCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcgac               <  2:1504739/93‑1 (MQ=255)

                                                                          aTCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcgac               <  2:3708888/93‑1 (MQ=255)

                                                                           tCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcgacg              >  1:2511740/1‑93 (MQ=255)

                                                                           tCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcgacg              >  2:3596020/1‑93 (MQ=255)

                                                                              ccAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAc           <  1:463373/93‑1 (MQ=255)

                                                                              ccAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAc           >  1:923821/1‑93 (MQ=255)

                                                                              ccAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAc           >  2:1117425/1‑93 (MQ=255)

                                                                                aGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAg         >  1:528841/1‑93 (MQ=255)

                                                                                aGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCCACGCACAg         >  2:2936408/1‑93 (MQ=255)

                                                                                  cgatCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACGGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCa       <  2:6321790/90‑1 (MQ=255)

                                                                                   ggTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAt      <  2:3181309/93‑1 (MQ=255)

                                                                                    gTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATc     >  1:4972146/1‑93 (MQ=255)

                                                                                     tCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCg    >  1:1437917/1‑93 (MQ=255)

                                                                                     tCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCg    <  2:5807260/93‑1 (MQ=255)

                                                                                       aGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGtt  <  1:5800388/93‑1 (MQ=255)

                                                                                       aGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGtt  >  1:6020955/1‑93 (MQ=255)

                                                                                       aGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGtt  <  2:1411094/93‑1 (MQ=255)

CACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTT  >  NC_000913/3823861‑3824040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGG  >  NC_000913/3823858‑3824044

TTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCAGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGC                                                                                               <  SRR4302534.730239/94‑1 (MQ=60)

 TGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCG                                                                                              <  SRR4302534.3872636/94‑1 (MQ=60)

  GCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGC                                                                                             <  SRR4302534.1805132/94‑1 (MQ=60)

  GCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGC                                                                                             >  SRR4302534.5872766/1‑94 (MQ=60)

    ACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAG                                                                                           >  SRR4302534.5613571/1‑94 (MQ=60)

      CGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGAT                                                                                         <  SRR4302534.4790246/94‑1 (MQ=60)

      CGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGAT                                                                                         >  SRR4302534.1127823/1‑94 (MQ=60)

      CGCTCGGTAGCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGAT                                                                                         <  SRR4302534.914808/94‑1 (MQ=60)

       GCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGCTG                                                                                        >  SRR4302534.1916075/1‑94 (MQ=60)

       GCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATG                                                                                        <  SRR4302534.5579463/94‑1 (MQ=60)

       GCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATG                                                                                        <  SRR4302534.3708404/94‑1 (MQ=60)

       GCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATG                                                                                        >  SRR4302534.1547980/1‑94 (MQ=60)

        CTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGA                                                                                       <  SRR4302534.4175395/94‑1 (MQ=60)

         TCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGAT                                                                                      <  SRR4302534.2936613/94‑1 (MQ=60)

         TCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGAT                                                                                      >  SRR4302534.6487192/1‑94 (MQ=60)

           GGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTG                                                                                    <  SRR4302534.4323570/94‑1 (MQ=60)

           GGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTG                                                                                    <  SRR4302534.5863973/94‑1 (MQ=60)

           GGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTG                                                                                    <  SRR4302534.453078/94‑1 (MQ=60)

           GGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTG                                                                                    >  SRR4302534.1065378/1‑94 (MQ=60)

           GGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTG                                                                                    >  SRR4302534.1128065/1‑94 (MQ=60)

            GTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGT                                                                                   >  SRR4302534.6340143/1‑94 (MQ=60)

            GTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGT                                                                                   >  SRR4302534.1364066/1‑94 (MQ=60)

            GTATCGAAGTGGGCTGGCGCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGT                                                                                   <  SRR4302534.1640779/94‑1 (MQ=60)

             TATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTC                                                                                  <  SRR4302534.4451605/94‑1 (MQ=60)

             TATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTC                                                                                  <  SRR4302534.3323122/94‑1 (MQ=60)

              ATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCG                                                                                 <  SRR4302534.5746090/94‑1 (MQ=60)

              ATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCG                                                                                 >  SRR4302534.3965395/1‑94 (MQ=60)

                CGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGT                                                                               >  SRR4302534.2422943/1‑94 (MQ=60)

                 GAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGCTTGTCGGTA                                                                              >  SRR4302534.5755985/1‑94 (MQ=60)

                 GAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTA                                                                              >  SRR4302534.3627974/1‑94 (MQ=60)

                   AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACA                                                                            <  SRR4302534.6550200/94‑1 (MQ=60)

                   AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACA                                                                            >  SRR4302534.1131809/1‑94 (MQ=60)

                   AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACA                                                                            <  SRR4302534.1644148/94‑1 (MQ=60)

                     TGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACA                                                                          <  SRR4302534.6354021/94‑1 (MQ=60)

                      GGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACAC                                                                         <  SRR4302534.293883/94‑1 (MQ=60)

                      GGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACAC                                                                         <  SRR4302534.2812131/94‑1 (MQ=60)

                      GGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACAC                                                                         >  SRR4302534.616091/1‑94 (MQ=60)

                      GGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGCTCAGGCGCAGATGATTGTCGGTACACAC                                                                         >  SRR4302534.2200023/1‑94 (MQ=60)

                       GGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACG                                                                        >  SRR4302534.6031545/1‑94 (MQ=60)

                         CTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCC                                                                      <  SRR4302534.119098/94‑1 (MQ=60)

                         CTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCC                                                                      <  SRR4302534.2336553/94‑1 (MQ=60)

                          TGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCA                                                                     <  SRR4302534.1789669/94‑1 (MQ=60)

                          TGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCA                                                                     <  SRR4302534.3587901/94‑1 (MQ=60)

                          TGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCA                                                                     <  SRR4302534.2248245/94‑1 (MQ=60)

                           GGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCGT                                                                    <  SRR4302534.5968529/94‑1 (MQ=60)

                           GGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCAT                                                                    >  SRR4302534.5031932/1‑94 (MQ=60)

                            GCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATC                                                                   <  SRR4302534.5774863/94‑1 (MQ=60)

                            GCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATC                                                                   <  SRR4302534.6281881/94‑1 (MQ=60)

                             CTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGGACACACGCCATCT                                                                  >  SRR4302534.953878/1‑94 (MQ=60)

                              TCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTT                                                                 <  SRR4302534.6505034/94‑1 (MQ=60)

                               CGCCGGTAATCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTC                                                                <  SRR4302534.870469/94‑1 (MQ=60)

                               CGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTC                                                                >  SRR4302534.3962299/1‑94 (MQ=60)

                                GCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCC                                                               <  SRR4302534.515117/94‑1 (MQ=60)

                                 CCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCA                                                              >  SRR4302534.3741947/1‑94 (MQ=60)

                                 CCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCCTCCA                                                              >  SRR4302534.1032232/1‑94 (MQ=60)

                                  CGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAG                                                             >  SRR4302534.736582/1‑94 (MQ=60)

                                   GGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGG                                                            <  SRR4302534.4353153/94‑1 (MQ=60)

                                      AAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAAC                                                         <  SRR4302534.5646286/94‑1 (MQ=60)

                                       AGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACA                                                        <  SRR4302534.4822481/94‑1 (MQ=60)

                                        GCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAG                                                       <  SRR4302534.4980684/94‑1 (MQ=60)

                                         CAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGG                                                      >  SRR4302534.4034881/1‑94 (MQ=60)

                                         CAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGG                                                      <  SRR4302534.6595794/94‑1 (MQ=60)

                                         CAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGG                                                      <  SRR4302534.800294/94‑1 (MQ=60)

                                         CAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGG                                                      >  SRR4302534.306204/1‑94 (MQ=60)

                                          AGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGT                                                     <  SRR4302534.3668585/94‑1 (MQ=60)

                                          AGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGT                                                     >  SRR4302534.3507063/1‑94 (MQ=60)

                                          AGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGT                                                     >  SRR4302534.4844145/1‑94 (MQ=60)

                                           GAAAGGTAAAGCACGGCTGGCACAGCAGGAGGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTG                                                    <  SRR4302534.867191/94‑1 (MQ=60)

                                           GAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTG                                                    <  SRR4302534.2255443/94‑1 (MQ=60)

                                            AAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGC                                                   >  SRR4302534.432318/1‑94 (MQ=60)

                                            AAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGC                                                   <  SRR4302534.5097020/94‑1 (MQ=60)

                                             AAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCA                                                  >  SRR4302534.5985719/1‑94 (MQ=60)

                                             AAGGTAAAGCACGGCTGACACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCA                                                  <  SRR4302534.5695147/94‑1 (MQ=60)

                                              AGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAG                                                 <  SRR4302534.547254/94‑1 (MQ=60)

                                              AGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAG                                                 >  SRR4302534.3470786/1‑94 (MQ=60)

                                              AGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGCGCAG                                                 >  SRR4302534.2254721/1‑94 (MQ=60)

                                               GGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGT                                                <  SRR4302534.3925664/94‑1 (MQ=60)

                                                 TAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTT                                              <  SRR4302534.2310626/94‑1 (MQ=60)

                                                  AAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTA                                             <  SRR4302534.6338649/94‑1 (MQ=60)

                                                  AAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTA                                             >  SRR4302534.2946023/1‑94 (MQ=60)

                                                  AAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTA                                             <  SRR4302534.4846957/94‑1 (MQ=60)

                                                  AAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACCCCATCTTCCAGGAACAGGTGCAGATTA                                             >  SRR4302534.1809507/1‑94 (MQ=60)

                                                       ACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGC                                        <  SRR4302534.4737713/94‑1 (MQ=60)

                                                        TGGCTGGCACAGCAGGAAGCCATCGCCAGGGGTCAGGGGCAGATGATTGTCGGTACACACGCCACCTTCCAGGAACAGGTGCAGTTTAACGGCC                                       >  SRR4302534.1736289/1‑94 (MQ=60)

                                                         GGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGAGCAGGTTAACGGCCT                                      >  SRR4302534.2491531/1‑94 (MQ=60)

                                                          GCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTG                                     <  SRR4302534.4236751/94‑1 (MQ=60)

                                                           CTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGG                                    <  SRR4302534.5905518/94‑1 (MQ=60)

                                                           CTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGG                                    <  SRR4302534.6202289/94‑1 (MQ=60)

                                                           CTAGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGG                                    >  SRR4302534.4450452/1‑94 (MQ=60)

                                                            TGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGAatgtttgttcacacgccaccatccaccaacaagagcagtttaatgggtctgg                                   >  SRR4302534.6365802/1‑42 (MQ=60)

                                                            TGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGC                                   <  SRR4302534.973476/94‑1 (MQ=60)

                                                               CACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCT                                >  SRR4302534.3652944/1‑94 (MQ=60)

                                                                ACAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTG                               >  SRR4302534.1945770/1‑94 (MQ=60)

                                                                ACAGCAGCAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGAAACAGGTGCAGTTTAACGGCCTGGCGCTG                               <  SRR4302534.135130/94‑1 (MQ=60)

                                                                 CAGCAGGAAGGCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGG                              <  SRR4302534.5890554/94‑1 (MQ=60)

                                                                 CAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGG                              >  SRR4302534.2795106/1‑94 (MQ=60)

                                                                 CAGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGG                              >  SRR4302534.4331698/1‑94 (MQ=60)

                                                                  AGCAGGAAGCCATCGCCAGCGGTCAGGCGCATATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTCCAGTTTAACGGCCTGGCGCTGGT                             <  SRR4302534.2293087/94‑1 (MQ=60)

                                                                  AGCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGG                             >  SRR4302534.4763982/1‑94 (MQ=60)

                                                                   GCAGTAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTG                            <  SRR4302534.4893164/94‑1 (MQ=60)

                                                                   GCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTG                            <  SRR4302534.2197497/94‑1 (MQ=60)

                                                                   GCAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAAAGGCCTGGCGCTGGTG                            >  SRR4302534.1232380/1‑94 (MQ=60)

                                                                    CAGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGA                           <  SRR4302534.2054917/94‑1 (MQ=60)

                                                                     AGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGAT                          >  SRR4302534.2534952/1‑94 (MQ=60)

                                                                     AGGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGAT                          <  SRR4302534.2773147/94‑1 (MQ=60)

                                                                      GGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATT                         >  SRR4302534.6403048/1‑94 (MQ=60)

                                                                      GGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATT                         <  SRR4302534.5576907/94‑1 (MQ=60)

                                                                      GGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATT                         >  SRR4302534.2448499/1‑94 (MQ=60)

                                                                      GGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATT                         <  SRR4302534.165630/94‑1 (MQ=60)

                                                                      GGAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATT                         >  SRR4302534.1406438/1‑94 (MQ=60)

                                                                       GAAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTA                        <  SRR4302534.488953/94‑1 (MQ=60)

                                                                        AAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGTCTGGCGCTGGTGATTAT                       >  SRR4302534.6537461/1‑94 (MQ=60)

                                                                        AAGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTAT                       <  SRR4302534.6264605/94‑1 (MQ=60)

                                                                         AGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATC                      <  SRR4302534.615686/94‑1 (MQ=60)

                                                                         AGCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATC                      <  SRR4302534.2755693/94‑1 (MQ=60)

                                                                          GCCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCG                     <  SRR4302534.3763019/94‑1 (MQ=60)

                                                                           CCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGA                    <  SRR4302534.3202216/94‑1 (MQ=60)

                                                                           CCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGA                    <  SRR4302534.3732696/94‑1 (MQ=60)

                                                                           CCATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGA                    >  SRR4302534.3923064/1‑94 (MQ=60)

                                                                            CATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGAC                   >  SRR4302534.3463313/1‑94 (MQ=60)

                                                                            CATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGAC                   <  SRR4302534.2707683/94‑1 (MQ=60)

                                                                             ATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                  >  SRR4302534.5917511/1‑94 (MQ=60)

                                                                             ATCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                  >  SRR4302534.2578423/1‑94 (MQ=60)

                                                                              TCGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGGCTGGCGCTGGTGATTATCGACGA                 >  SRR4302534.810868/1‑94 (MQ=60)

                                                                               CGCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACTGCCTGGCGCTGGTGATTATCGACGAA                >  SRR4302534.2459948/1‑94 (MQ=60)

                                                                                GCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAC               >  SRR4302534.947346/1‑94 (MQ=60)

                                                                                GCCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAC               <  SRR4302534.916357/94‑1 (MQ=60)

                                                                                 CCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACA              <  SRR4302534.475071/94‑1 (MQ=60)

                                                                                 CCAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACA              >  SRR4302534.3678718/1‑94 (MQ=60)

                                                                                  CAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAG             >  SRR4302534.541973/1‑94 (MQ=60)

                                                                                  CAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAG             >  SRR4302534.2394664/1‑94 (MQ=60)

                                                                                  CAGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAG             <  SRR4302534.1433680/94‑1 (MQ=60)

                                                                                   AGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGC            >  SRR4302534.5148193/1‑94 (MQ=60)

                                                                                   AGCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGC            >  SRR4302534.6391924/1‑94 (MQ=60)

                                                                                    GCGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCA           <  SRR4302534.5041726/94‑1 (MQ=60)

                                                                                     CGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT          <  SRR4302534.4300749/94‑1 (MQ=60)

                                                                                     CGGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT          <  SRR4302534.1526082/94‑1 (MQ=60)

                                                                                      GGTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATC         >  SRR4302534.5106751/1‑94 (MQ=60)

                                                                                       GTCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCG        >  SRR4302534.1475771/1‑94 (MQ=60)

                                                                                       GCCAGGCGCAGATGATTGTCGGTACACACGCCCTCTTCCAGGTACAGGTGCAGTTTAACGGCCTGGCGCTGGTGTTTATCGACGAACTGCATCG        >  SRR4302534.3314639/1‑94 (MQ=60)

                                                                                        TCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGT       <  SRR4302534.2541841/94‑1 (MQ=60)

                                                                                        TCAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGT       >  SRR4302534.4683919/1‑94 (MQ=60)

                                                                                        TCAGACGCAGATGATTTTCGGTACACACGCCATCTTCCAGGAACAGATGCAGTTTAACGGCCTGACGCTGGTGATTATCGACGAACAGCATCGT       <  SRR4302534.4445823/94‑1 (MQ=60)

                                                                                         CAGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTT      >  SRR4302534.6184580/1‑94 (MQ=60)

                                                                                          AGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATGGTTT     >  SRR4302534.4339481/1‑94 (MQ=60)

                                                                                          AGGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTT     <  SRR4302534.5957793/94‑1 (MQ=60)

                                                                                           GGCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTT    >  SRR4302534.2202831/1‑94 (MQ=60)

                                                                                            GCGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTG   <  SRR4302534.3727970/94‑1 (MQ=60)

                                                                                             CGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGG  <  SRR4302534.148931/94‑1 (MQ=60)

                                                                                             CGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGG  <  SRR4302534.4652704/94‑1 (MQ=60)

TTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGG  >  NC_000913/3823858‑3824044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`