Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,836,443	G→A	G190D (GGC→GAC)	yicL →	putative permease transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,836,443	0	G	A	99.6%	994.1 / ‑5.2	259	G190D (GGC→GAC)	yicL	putative permease transporter
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (121/137); total (121/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

Only 100 of 266 total aligned reads displayed.

TCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT  >  NC_000913/3836353‑3836535

tCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACt                                                                                            <  2:5687697/93‑1 (MQ=255)

 cTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTg                                                                                           <  1:2636842/93‑1 (MQ=255)

      gCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTa                                                                                      <  1:5174352/93‑1 (MQ=255)

      gCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTa                                                                                      <  1:5885304/93‑1 (MQ=255)

      gCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTa                                                                                      >  1:3684449/1‑93 (MQ=255)

       cATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTAt                                                                                     <  1:4141317/93‑1 (MQ=255)

        aTTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATg                                                                                    >  1:1213154/1‑93 (MQ=255)

         ttGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGc                                                                                   >  2:2443781/1‑93 (MQ=255)

          tGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCt                                                                                  <  2:3771042/93‑1 (MQ=255)

           gCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTg                                                                                 >  2:1904350/1‑93 (MQ=255)

           gCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTg                                                                                 >  1:1851683/1‑93 (MQ=255)

           gCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTg                                                                                 >  2:174308/1‑93 (MQ=255)

            ccTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGa                                                                                <  1:2766023/93‑1 (MQ=255)

              tCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGAtt                                                                              <  2:515492/93‑1 (MQ=255)

               cGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTg                                                                             <  1:745334/93‑1 (MQ=255)

               cGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTg                                                                             <  1:5378453/93‑1 (MQ=255)

               cGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTg                                                                             <  1:162706/93‑1 (MQ=255)

                 gCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGc                                                                           <  1:2410285/93‑1 (MQ=255)

                 gCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGc                                                                           >  1:2888037/1‑93 (MQ=255)

                 gCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGc                                                                           >  1:5342237/1‑93 (MQ=255)

                  cATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCg                                                                          <  1:4605153/93‑1 (MQ=255)

                   aTTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCgg                                                                         >  1:1436607/1‑93 (MQ=255)

                    tttGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGt                                                                        >  2:1022542/1‑93 (MQ=255)

                    tttGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGt                                                                        <  1:2277743/93‑1 (MQ=255)

                       gctgctgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTg                                                                     <  2:135051/93‑1 (MQ=255)

                        ctgctgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGa                                                                    <  1:3849990/93‑1 (MQ=255)

                         tgctgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGAt                                                                   >  2:4769056/1‑93 (MQ=255)

                           ctgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTc                                                                 <  2:2775098/93‑1 (MQ=255)

                            tgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCt                                                                >  2:5364873/1‑93 (MQ=255)

                            tgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCt                                                                >  1:5778398/1‑93 (MQ=255)

                            tgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCt                                                                >  2:1677557/1‑93 (MQ=255)

                            tgcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCt                                                                <  2:6232065/93‑1 (MQ=255)

                             gcATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTg                                                               <  1:936971/93‑1 (MQ=255)

                               aTTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGtt                                                             <  1:4260968/93‑1 (MQ=255)

                                ttCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTg                                                            >  2:155780/1‑93 (MQ=255)

                                  cTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGcc                                                          <  1:4936720/93‑1 (MQ=255)

                                   tataCCACCTATCCTTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGCGTATGCTGATTGGTGGTCTGATTCTGTTGCCt                                                         >  2:1045963/1‑93 (MQ=255)

                                   tataCCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCt                                                         >  2:1712618/1‑93 (MQ=255)

                                   tataCCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCt                                                         <  2:303938/93‑1 (MQ=255)

                                    ataCCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCtt                                                        >  2:5511728/1‑93 (MQ=255)

                                     taCCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCttt                                                       <  1:2059908/93‑1 (MQ=255)

                                     taCCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCttt                                                       <  1:6314787/93‑1 (MQ=255)

                                     taCCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCttt                                                       <  1:3169032/93‑1 (MQ=255)

                                       ccaccTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCttttt                                                     <  2:3520669/93‑1 (MQ=255)

                                        caccTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTa                                                    <  2:347036/93‑1 (MQ=255)

                                        caccTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTa                                                    >  1:3488807/1‑93 (MQ=255)

                                         cccTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTAt                                                   <  2:4055682/92‑1 (MQ=255)

                                           cTATCCATCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTTGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGc                                                 <  2:2843641/93‑1 (MQ=255)

                                            tATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGcc                                                >  1:5441399/1‑93 (MQ=255)

                                             aTCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCa                                               >  1:5251259/1‑93 (MQ=255)

                                             aTCCCTCAACGCTAATTGCCAGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCa                                               >  2:3167176/1‑93 (MQ=255)

                                               cccTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGa                                             <  1:4972432/93‑1 (MQ=255)

                                                ccTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGAc                                            >  1:2919122/1‑93 (MQ=255)

                                                 cTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACa                                           >  2:2661749/1‑93 (MQ=255)

                                                   cAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAg                                         <  1:2629630/93‑1 (MQ=255)

                                                    aaCGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAgg                                        <  2:4141662/93‑1 (MQ=255)

                                                     aCGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGa                                       >  2:3339817/1‑93 (MQ=255)

                                                      cGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGaa                                      >  1:3756986/1‑93 (MQ=255)

                                                       gCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAAc                                     <  2:4950253/93‑1 (MQ=255)

                                                       gCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAAc                                     <  2:977453/93‑1 (MQ=255)

                                                         tAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACaa                                   <  1:2973767/93‑1 (MQ=255)

                                                          aaTTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACaaa                                  <  1:1682418/93‑1 (MQ=255)

                                                           aTTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAAc                                 >  1:6422374/1‑93 (MQ=255)

                                                           aTTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAAc                                 >  1:5517549/1‑93 (MQ=255)

                                                              gCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACttt                              <  1:680921/93‑1 (MQ=255)

                                                                ccGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGt                            <  1:1781220/93‑1 (MQ=255)

                                                                ccGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGt                            <  1:4370896/93‑1 (MQ=255)

                                                                  gCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCg                          <  1:1063361/93‑1 (MQ=255)

                                                                   cTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGTTTGGCGGTCTGATTCTGTTGCCCTTTTATGCCAGACAAGGAACAAACTTTGTCGt                         >  2:2143333/1‑93 (MQ=255)

                                                                    tATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGtt                        <  2:3996443/93‑1 (MQ=255)

                                                                    tATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGtt                        >  1:4170071/1‑93 (MQ=255)

                                                                     aTGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTa                       <  2:4979063/93‑1 (MQ=255)

                                                                     aTGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTa                       <  1:6250792/93‑1 (MQ=255)

                                                                      tGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTaa                      >  2:5959500/1‑93 (MQ=255)

                                                                      tGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTaa                      <  1:3310270/93‑1 (MQ=255)

                                                                        gCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACg                    <  1:2605398/93‑1 (MQ=255)

                                                                          aCGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGc                  <  1:4688772/93‑1 (MQ=255)

                                                                          aCGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGc                  >  1:218698/1‑93 (MQ=255)

                                                                            gTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAg                <  2:1147880/93‑1 (MQ=255)

                                                                            gTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAg                <  2:652619/93‑1 (MQ=255)

                                                                            gTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAg                <  1:2933076/93‑1 (MQ=255)

                                                                             ttACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGt               <  1:4455378/93‑1 (MQ=255)

                                                                             ttACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGt               >  1:6112621/1‑93 (MQ=255)

                                                                              tACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGtt              <  2:3248383/93‑1 (MQ=255)

                                                                              tACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGtt              >  2:5728525/1‑93 (MQ=255)

                                                                              tACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGtt              >  2:2736545/1‑93 (MQ=255)

                                                                              tACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGtt              <  1:2719270/93‑1 (MQ=255)

                                                                               aCCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGttt             >  2:5451911/1‑93 (MQ=255)

                                                                               aCCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGttt             <  1:5625745/93‑1 (MQ=255)

                                                                               aCCAGTCGTCGAATGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGttt             <  2:2055050/93‑1 (MQ=255)

                                                                                ccAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTg            >  2:2494177/1‑93 (MQ=255)

                                                                                 cAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGa           >  2:770622/1‑93 (MQ=255)

                                                                                  aGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGAt          <  2:4872382/93‑1 (MQ=255)

                                                                                  aGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGAt          <  2:592709/93‑1 (MQ=255)

                                                                                   gtcgtcGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGAtt         >  1:3533566/1‑93 (MQ=255)

                                                                                    tcgtcgACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTc        >  2:4605147/1‑93 (MQ=255)

                                                                                     cgtcgACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCt       <  2:4831023/93‑1 (MQ=255)

                                                                                     cgtcgACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCt       <  2:3374118/93‑1 (MQ=255)

                                                                                      gtcgACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTg      <  2:5786213/93‑1 (MQ=255)

                                                                                       tcgACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAGCTTTGTCGTTAACGGCAGTTTGATTCTgg     <  1:3135620/93‑1 (MQ=255)

TCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT  >  NC_000913/3836353‑3836535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTT  >  NC_000913/3836350‑3836536

TGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGA                                                                                               <  SRR4302534.2221180/94‑1 (MQ=60)

  TTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACT                                                                                             <  SRR4302534.2836033/94‑1 (MQ=60)

  TTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACT                                                                                             <  SRR4302534.6463476/94‑1 (MQ=60)

  TTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACT                                                                                             <  SRR4302534.4781143/94‑1 (MQ=60)

   TCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTG                                                                                            <  SRR4302534.3214071/94‑1 (MQ=60)

   TCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTG                                                                                            >  SRR4302534.3320060/1‑94 (MQ=60)

    CTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGG                                                                                           <  SRR4302534.2706601/94‑1 (MQ=60)

     TGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGG                                                                                          >  SRR4302534.2439195/1‑94 (MQ=60)

     TGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGA                                                                                          <  SRR4302534.869465/94‑1 (MQ=60)

      GGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAG                                                                                         <  SRR4302534.709418/94‑1 (MQ=60)

      GGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAG                                                                                         <  SRR4302534.265304/94‑1 (MQ=60)

      GGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAG                                                                                         <  SRR4302534.6510631/94‑1 (MQ=60)

        GGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTA                                                                                       >  SRR4302534.3445636/1‑94 (MQ=60)

        GGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTA                                                                                       >  SRR4302534.3783510/1‑94 (MQ=60)

         GCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTAT                                                                                      <  SRR4302534.5313796/94‑1 (MQ=60)

         GCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTAT                                                                                      >  SRR4302534.4729363/1‑94 (MQ=60)

         GCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTAT                                                                                      <  SRR4302534.6045099/94‑1 (MQ=60)

         GCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTAT                                                                                      <  SRR4302534.3827721/94‑1 (MQ=60)

          CATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATG                                                                                     >  SRR4302534.1245089/1‑94 (MQ=60)

          CATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATG                                                                                     <  SRR4302534.4253075/94‑1 (MQ=60)

           ATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGC                                                                                    <  SRR4302534.5275260/94‑1 (MQ=60)

           ATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGC                                                                                    >  SRR4302534.3018887/1‑94 (MQ=60)

           ATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGC                                                                                    >  SRR4302534.3779064/1‑94 (MQ=60)

             TGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTG                                                                                  >  SRR4302534.1899850/1‑94 (MQ=60)

              GCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGA                                                                                 >  SRR4302534.2442815/1‑94 (MQ=60)

               CCTCGGCATTTGGTGCTGTATTCTATACCTACTATCCCGCAGCGCTAATTGCCCGCTGTGGCACGTTACCAGTCGTCGACTGGAGTATGCTGAT                                                                                <  SRR4302534.2161043/94‑1 (MQ=60)

               CCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGAT                                                                                <  SRR4302534.2838841/94‑1 (MQ=60)

                CTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATT                                                                               >  SRR4302534.6024409/1‑94 (MQ=60)

                 TCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTG                                                                              <  SRR4302534.2036054/94‑1 (MQ=60)

                 TCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTG                                                                              <  SRR4302534.4787406/94‑1 (MQ=60)

                 TCGGCATTTGCTGCTGCACTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTG                                                                              >  SRR4302534.1355552/1‑94 (MQ=60)

                  CGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGG                                                                             <  SRR4302534.603574/94‑1 (MQ=60)

                  CGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGG                                                                             <  SRR4302534.1850477/94‑1 (MQ=60)

                  CGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGG                                                                             <  SRR4302534.5523360/94‑1 (MQ=60)

                  CGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGG                                                                             <  SRR4302534.166968/94‑1 (MQ=60)

                  CGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGG                                                                             <  SRR4302534.763806/94‑1 (MQ=60)

                   GGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGC                                                                            >  SRR4302534.5486267/1‑94 (MQ=60)

                   GGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGC                                                                            >  SRR4302534.2963815/1‑94 (MQ=60)

                    GCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCG                                                                           <  SRR4302534.133064/94‑1 (MQ=60)

                    GCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCG                                                                           <  SRR4302534.2474375/94‑1 (MQ=60)

                    GCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCG                                                                           <  SRR4302534.3826630/94‑1 (MQ=60)

                     CATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGG                                                                          <  SRR4302534.4730121/94‑1 (MQ=60)

                     CATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGG                                                                          >  SRR4302534.1474418/1‑94 (MQ=60)

                     CATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGG                                                                          >  SRR4302534.5304811/1‑94 (MQ=60)

                       TTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTC                                                                        >  SRR4302534.4431202/1‑94 (MQ=60)

                       TTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTC                                                                        <  SRR4302534.2338184/94‑1 (MQ=60)

                        TTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCT                                                                       <  SRR4302534.533480/94‑1 (MQ=60)

                        TTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCT                                                                       >  SRR4302534.4421171/1‑94 (MQ=60)

                        TTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCG                                                                       <  SRR4302534.1118266/94‑1 (MQ=60)

                         TGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTG                                                                      >  SRR4302534.2812311/1‑94 (MQ=60)

                           CTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGAT                                                                    <  SRR4302534.3953370/94‑1 (MQ=60)

                           CTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGAT                                                                    >  SRR4302534.2692744/1‑94 (MQ=60)

                            TGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATT                                                                   <  SRR4302534.2139145/94‑1 (MQ=60)

                            TGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATT                                                                   >  SRR4302534.1690789/1‑94 (MQ=60)

                            TGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATT                                                                   <  SRR4302534.5321045/94‑1 (MQ=60)

                              CTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCT                                                                 >  SRR4302534.5935135/1‑94 (MQ=60)

                                GCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGT                                                               <  SRR4302534.960800/94‑1 (MQ=60)

                                 CATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTT                                                              <  SRR4302534.3697479/94‑1 (MQ=60)

                                 CATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTT                                                              <  SRR4302534.1807521/94‑1 (MQ=60)

                                  ATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTG                                                             >  SRR4302534.5489390/1‑94 (MQ=60)

                                  ATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTG                                                             <  SRR4302534.4376053/94‑1 (MQ=60)

                                    TCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCC                                                           <  SRR4302534.3872909/94‑1 (MQ=60)

                                     CTATACCACCTATCCCTCAACGCTGATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCT                                                          <  SRR4302534.6515059/94‑1 (MQ=60)

                                     CTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCT                                                          <  SRR4302534.5070501/94‑1 (MQ=60)

                                      TATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTT                                                         >  SRR4302534.6472882/1‑94 (MQ=60)

                                      TATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTT                                                         >  SRR4302534.15939/1‑94 (MQ=60)

                                       ATACCACCTATCCCTCAACGCTAATTGTCCGCTATAGCACCTTACCAGCCGTCGACTGGCGAATGGTGATTGGCGGTCTGGTTCTGTTGCCTTT                                                        >  SRR4302534.3416584/1‑94 (MQ=53)

                                        tactcCCTATCCCGCAACGCGAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTT                                                       <  SRR4302534.4044919/89‑1 (MQ=60)

                                        TACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTT                                                       <  SRR4302534.2113756/94‑1 (MQ=60)

                                        TACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTT                                                       <  SRR4302534.3252621/94‑1 (MQ=60)

                                        TACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTT                                                       <  SRR4302534.6485474/94‑1 (MQ=60)

                                        TACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTT                                                       <  SRR4302534.2984571/94‑1 (MQ=60)

                                          CCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTA                                                     >  SRR4302534.3582576/1‑94 (MQ=60)

                                          CCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTA                                                     >  SRR4302534.2721645/1‑94 (MQ=60)

                                          CCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTA                                                     <  SRR4302534.6640348/94‑1 (MQ=60)

                                           CACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTAT                                                    <  SRR4302534.4018988/94‑1 (MQ=60)

                                              CTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCC                                                 >  SRR4302534.312355/1‑94 (MQ=60)

                                              CTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCC                                                 >  SRR4302534.5587975/1‑94 (MQ=60)

                                              CTAGCCCTCAGCGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCC                                                 <  SRR4302534.5856965/94‑1 (MQ=60)

                                               TATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCA                                                >  SRR4302534.5393137/1‑94 (MQ=60)

                                               TATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCA                                                >  SRR4302534.5025242/1‑94 (MQ=60)

                                               TATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCA                                                <  SRR4302534.1804706/94‑1 (MQ=60)

                                                  CCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGAC                                             >  SRR4302534.2995723/1‑94 (MQ=60)

                                                  CCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGAC                                             <  SRR4302534.5107048/94‑1 (MQ=60)

                                                  CCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGAC                                             >  SRR4302534.269880/1‑94 (MQ=60)

                                                   CCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACA                                            <  SRR4302534.2713669/94‑1 (MQ=60)

                                                    CTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAA                                           >  SRR4302534.2295654/1‑94 (MQ=60)

                                                    CTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAA                                           >  SRR4302534.5488433/1‑94 (MQ=60)

                                                    CTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAA                                           >  SRR4302534.4549754/1‑94 (MQ=60)

                                                     TCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAG                                          <  SRR4302534.5594639/94‑1 (MQ=60)

                                                      CAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGG                                         <  SRR4302534.2699186/94‑1 (MQ=60)

                                                      CAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGG                                         <  SRR4302534.5578185/94‑1 (MQ=60)

                                                       AACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGA                                        >  SRR4302534.2905367/1‑94 (MQ=60)

                                                        ACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAA                                       >  SRR4302534.3857864/1‑94 (MQ=60)

                                                         CGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAAC                                      >  SRR4302534.4278271/1‑94 (MQ=60)

                                                          GCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACA                                     <  SRR4302534.4868284/94‑1 (MQ=60)

                                                            TAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAA                                   <  SRR4302534.3590424/94‑1 (MQ=60)

                                                            TAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAA                                   >  SRR4302534.3334849/1‑94 (MQ=60)

                                                            TAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAA                                   <  SRR4302534.3051614/94‑1 (MQ=60)

                                                            TAATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAA                                   <  SRR4302534.5743871/94‑1 (MQ=60)

                                                             AATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAAC                                  >  SRR4302534.6595589/1‑94 (MQ=60)

                                                             AATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAAC                                  >  SRR4302534.5666431/1‑94 (MQ=60)

                                                             AATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAAC                                  <  SRR4302534.1726177/94‑1 (MQ=60)

                                                             AATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGAAAAGGAACAAAC                                  >  SRR4302534.2867522/1‑94 (MQ=60)

                                                              ATTGCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACT                                 >  SRR4302534.5879271/1‑94 (MQ=60)

                                                                 GCCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTG                              <  SRR4302534.697551/94‑1 (MQ=60)

                                                                  CCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGT                             >  SRR4302534.2573739/1‑94 (MQ=60)

                                                                  CCCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGT                             >  SRR4302534.4366718/1‑94 (MQ=60)

                                                                   CCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTC                            >  SRR4302534.660438/1‑94 (MQ=60)

                                                                   CCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTC                            <  SRR4302534.1827468/94‑1 (MQ=60)

                                                                   CCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTC                            <  SRR4302534.4497583/94‑1 (MQ=60)

                                                                   CCGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTC                            <  SRR4302534.4488664/94‑1 (MQ=60)

                                                                    CGCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCG                           <  SRR4302534.986702/94‑1 (MQ=60)

                                                                     GCTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGT                          <  SRR4302534.1090923/94‑1 (MQ=60)

                                                                      CTATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTT                         >  SRR4302534.4282715/1‑94 (MQ=60)

                                                                       TATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTA                        <  SRR4302534.3426340/94‑1 (MQ=60)

                                                                        ATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAA                       <  SRR4302534.1676381/94‑1 (MQ=60)

                                                                        ATGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAA                       <  SRR4302534.6420278/94‑1 (MQ=60)

                                                                         TGGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAAC                      <  SRR4302534.3398675/94‑1 (MQ=60)

                                                                          GGCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACG                     >  SRR4302534.6326029/1‑94 (MQ=60)

                                                                           GCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGG                    <  SRR4302534.2674338/94‑1 (MQ=60)

                                                                           GCACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGG                    <  SRR4302534.2979766/94‑1 (MQ=60)

                                                                            CACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGC                   >  SRR4302534.224431/1‑94 (MQ=60)

                                                                             CCGTTGCCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCTTTAACGGCA                  <  SRR4302534.3030896/94‑1 (MQ=60)

                                                                             ACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCA                  <  SRR4302534.4816037/94‑1 (MQ=60)

                                                                             ACGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCA                  <  SRR4302534.6159005/94‑1 (MQ=60)

                                                                              CGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAG                 >  SRR4302534.5449262/1‑94 (MQ=60)

                                                                              CGTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAG                 <  SRR4302534.2996242/94‑1 (MQ=60)

                                                                               GTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGT                <  SRR4302534.3010065/94‑1 (MQ=60)

                                                                               GTTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGT                >  SRR4302534.6278907/1‑94 (MQ=60)

                                                                                TTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTT               <  SRR4302534.3250749/94‑1 (MQ=60)

                                                                                TTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTT               <  SRR4302534.4575815/94‑1 (MQ=60)

                                                                                TTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGGT               >  SRR4302534.2703343/1‑94 (MQ=60)

                                                                                TTACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGCCGTTAACGGCAGTT               <  SRR4302534.6370638/94‑1 (MQ=60)

                                                                                 TACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTT              <  SRR4302534.2791028/94‑1 (MQ=60)

                                                                                 TACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTT              >  SRR4302534.893120/1‑94 (MQ=60)

                                                                                 TACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTT              <  SRR4302534.5581895/94‑1 (MQ=60)

                                                                                 TACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTT              <  SRR4302534.4784290/94‑1 (MQ=60)

                                                                                  ACCAGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTG             <  SRR4302534.5777995/94‑1 (MQ=60)

                                                                                     AGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATT          >  SRR4302534.3628619/1‑94 (MQ=60)

                                                                                     AGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATT          <  SRR4302534.5102634/94‑1 (MQ=60)

                                                                                     AGTCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATT          <  SRR4302534.5756791/94‑1 (MQ=60)

                                                                                       TCGTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCT        <  SRR4302534.4110970/94‑1 (MQ=60)

                                                                                        CGTCGACTGGAGTATGCTTATTGGCGTTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTG       <  SRR4302534.2130191/94‑1 (MQ=60)

                                                                                         GTCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGG      >  SRR4302534.1972405/1‑94 (MQ=60)

                                                                                          TCGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAGCTTTGTCGTTAACGGCAGTTTGATTCTGGC     <  SRR4302534.3218081/94‑1 (MQ=60)

                                                                                           CGACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCG    >  SRR4302534.1956333/1‑94 (MQ=60)

                                                                                            GACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT   >  SRR4302534.2605501/1‑94 (MQ=60)

                                                                                            GACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT   <  SRR4302534.5562876/94‑1 (MQ=60)

                                                                                             GCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTT  <  SRR4302534.425252/94‑1 (MQ=60)

                                                                                             ACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTT  <  SRR4302534.4226757/94‑1 (MQ=60)

                                                                                             ACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTT  <  SRR4302534.5223061/94‑1 (MQ=60)

                                                                                             ACTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTT  <  SRR4302534.3799684/94‑1 (MQ=60)

TGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTT  >  NC_000913/3836350‑3836536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`