Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,867,245	T→C	V88A (GTC→GCC)	yidS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,867,245	0	T	C	100.0%	984.4 / NA	285	V88A (GTC→GCC)	yidS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (126/159); total (126/159)

Only 100 of 299 total aligned reads displayed.

CACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGA  >  NC_000913/3867155‑3867334

 aCCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGa                                                                                        >  2:5683098/1‑93 (MQ=255)

  ccGGACGCACAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGAc                                                                                       <  1:5483783/93‑1 (MQ=255)

   cGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACg                                                                                      >  2:786425/1‑93 (MQ=255)

       cgcgCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTcgc                                                                                  <  2:338008/93‑1 (MQ=255)

       cgcgCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTcgc                                                                                  <  2:301471/93‑1 (MQ=255)

        gcgcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTcgcc                                                                                 <  1:204191/93‑1 (MQ=255)

           cAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCa                                                                              <  1:1177217/93‑1 (MQ=255)

           cAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCa                                                                              >  2:5467225/1‑93 (MQ=255)

           cAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCa                                                                              <  2:1027561/93‑1 (MQ=255)

           cAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCa                                                                              <  2:1243694/93‑1 (MQ=255)

            aGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGGCAAAACCGCCGACGTCGCCGCAt                                                                             <  2:72793/93‑1 (MQ=255)

             gCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATc                                                                            >  1:3813962/1‑93 (MQ=255)

             gCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATc                                                                            <  1:418928/93‑1 (MQ=255)

              cGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCg                                                                           <  1:5967463/93‑1 (MQ=255)

               gATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGc                                                                          >  2:3516332/1‑93 (MQ=255)

               gATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGc                                                                          <  1:1708588/93‑1 (MQ=255)

               gATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGc                                                                          >  2:624282/1‑93 (MQ=255)

                aTCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCt                                                                         >  1:1075291/1‑93 (MQ=255)

                    tttATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCAcg                                                                     >  2:1956380/1‑93 (MQ=255)

                     ttATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCAcgc                                                                    >  1:5906331/1‑93 (MQ=255)

                     ttATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCAcgc                                                                    <  2:3682838/93‑1 (MQ=255)

                        ttCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTa                                                                 <  2:2261305/93‑1 (MQ=255)

                        ttCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTa                                                                 <  1:2831605/93‑1 (MQ=255)

                        ttCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTa                                                                 <  1:5706633/93‑1 (MQ=255)

                          cgcgATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAAc                                                               >  1:5331063/1‑93 (MQ=255)

                           gcgATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACt                                                              <  2:2618238/93‑1 (MQ=255)

                            cgATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTa                                                             <  2:5331589/93‑1 (MQ=255)

                              aTGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTAcc                                                           <  2:1623322/93‑1 (MQ=255)

                              aTGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTAcc                                                           <  2:6437415/93‑1 (MQ=255)

                              aTGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGGAAGTAca                                                           >  1:1497042/1‑92 (MQ=255)

                               tGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCa                                                          <  1:5006691/93‑1 (MQ=255)

                                gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAg                                                         <  2:1504388/93‑1 (MQ=255)

                                gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAg                                                         <  2:6081141/93‑1 (MQ=255)

                                 ggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGc                                                        <  2:2724105/93‑1 (MQ=255)

                                 ggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGc                                                        >  1:338550/1‑93 (MQ=255)

                                 ggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGc                                                        >  2:6296610/1‑93 (MQ=255)

                                 ggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGc                                                        <  1:3214473/93‑1 (MQ=255)

                                    tGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTAGCAGCGaa                                                     <  1:2295525/93‑1 (MQ=255)

                                    tGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGaa                                                     >  1:5089326/1‑93 (MQ=255)

                                    tGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGaa                                                     >  2:1672087/1‑93 (MQ=255)

                                     gACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAg                                                    <  2:3196978/93‑1 (MQ=255)

                                     gACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAg                                                    >  1:4337744/1‑93 (MQ=255)

                                     gACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAg                                                    <  1:4577694/93‑1 (MQ=255)

                                     gACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAg                                                    >  1:4129718/1‑93 (MQ=255)

                                      aCGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGc                                                   >  2:2390374/1‑93 (MQ=255)

                                      aCGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGc                                                   <  1:474875/93‑1 (MQ=255)

                                       cGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCt                                                  >  2:4925978/1‑93 (MQ=255)

                                         cTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtat                                                <  2:2826819/93‑1 (MQ=255)

                                         cTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtat                                                <  1:3288342/93‑1 (MQ=255)

                                         cTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtat                                                <  1:2677372/93‑1 (MQ=255)

                                         cTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAACCtat                                                >  1:886640/1‑93 (MQ=255)

                                           tCCTGTCGTTGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAGCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTatat                                              <  1:3558815/93‑1 (MQ=255)

                                           tCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTatat                                              >  2:5934122/1‑93 (MQ=255)

                                           tCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTatat                                              <  2:1735059/93‑1 (MQ=255)

                                            ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATc                                             <  1:2608739/93‑1 (MQ=255)

                                            ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATc                                             <  2:892673/93‑1 (MQ=255)

                                               gTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAat                                          <  1:4044447/93‑1 (MQ=255)

                                               gTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAat                                          >  2:2493745/1‑93 (MQ=255)

                                               gTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAat                                          <  1:5802789/93‑1 (MQ=255)

                                                 cGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAatat                                        <  2:2157335/93‑1 (MQ=255)

                                                   aTGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTa                                      >  1:3420093/1‑93 (MQ=255)

                                                    tgtgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTaa                                     <  1:825185/93‑1 (MQ=255)

                                                     gtgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAAt                                    <  1:449802/93‑1 (MQ=255)

                                                     gtgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAAt                                    <  1:700466/93‑1 (MQ=255)

                                                     gtgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAAt                                    <  1:643561/93‑1 (MQ=255)

                                                       gATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCg                                  >  2:4477096/1‑93 (MQ=255)

                                                        aTCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGc                                 >  1:2506662/1‑93 (MQ=255)

                                                         tCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGcc                                <  2:141004/93‑1 (MQ=255)

                                                          cGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCa                               <  2:1260804/93‑1 (MQ=255)

                                                           gCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCAt                              >  1:3159514/1‑93 (MQ=255)

                                                           gCTAATCCGCAGATTTGCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCAt                              <  1:4137590/93‑1 (MQ=255)

                                                             tAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGc                            >  2:6042253/1‑93 (MQ=255)

                                                             tAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGc                            >  2:3305733/1‑93 (MQ=255)

                                                              aaTCGGCAGATTTTCAGCGTCAAAGCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCt                           <  2:3263429/93‑1 (MQ=255)

                                                               aTCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCtt                          <  1:4412748/93‑1 (MQ=255)

                                                                 ccGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTc                        >  1:1013574/1‑93 (MQ=255)

                                                                  cGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCg                       >  1:5107007/1‑93 (MQ=255)

                                                                   gCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGa                      >  1:1381920/1‑93 (MQ=255)

                                                                   gCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGa                      >  2:4169901/1‑93 (MQ=255)

                                                                    cAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAc                     >  2:4303404/1‑93 (MQ=255)

                                                                    cAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAc                     <  1:960844/93‑1 (MQ=255)

                                                                     aGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACt                    <  2:3649128/93‑1 (MQ=255)

                                                                      gATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACtt                   >  2:5983828/1‑93 (MQ=255)

                                                                        ttttCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgt                 <  2:6148388/93‑1 (MQ=255)

                                                                         tttCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgtg                <  1:6129203/93‑1 (MQ=255)

                                                                           tCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAGCTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGa              <  1:3920110/93‑1 (MQ=255)

                                                                            cAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGAt             <  2:5257271/93‑1 (MQ=255)

                                                                            cAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGAt             <  2:5031607/93‑1 (MQ=255)

                                                                            cAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGAt             <  1:5616645/93‑1 (MQ=255)

                                                                              gCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGa           >  1:5187797/1‑93 (MQ=255)

                                                                                gTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGaaa         <  2:5105199/93‑1 (MQ=255)

                                                                                gTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGaaa         <  2:1171989/93‑1 (MQ=255)

                                                                                gTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGaaa         <  2:1781260/93‑1 (MQ=255)

                                                                                gTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGaaa         >  1:193146/1‑93 (MQ=255)

                                                                                 tCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGGCATGCTTTCGACTTGTGGATGAAAt        >  1:2465264/1‑93 (MQ=255)

                                                                                    aaaCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGc     >  1:2736596/1‑93 (MQ=255)

                                                                                     aaCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCt    <  2:1042680/93‑1 (MQ=255)

                                                                                     aaCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCt    <  1:6285047/93‑1 (MQ=255)

                                                                                       ccgccgACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGa  >  2:4712652/1‑93 (MQ=255)

                                                                                       ccgccgACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGa  >  2:3798410/1‑93 (MQ=255)

CACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGA  >  NC_000913/3867155‑3867334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCC  >  NC_000913/3867154‑3867338

GCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCG                                                                                             >  SRR4302534.5288919/1‑94 (MQ=60)

 CACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGA                                                                                            >  SRR4302534.3494832/1‑94 (MQ=60)

   CCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACG                                                                                          <  SRR4302534.1421427/94‑1 (MQ=60)

   CCGGACGCACAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACG                                                                                          <  SRR4302534.5631678/94‑1 (MQ=60)

    CGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGT                                                                                         <  SRR4302534.5238685/94‑1 (MQ=60)

     GGACGCGCAGCGATCTTTTAGTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTC                                                                                        <  SRR4302534.4780879/94‑1 (MQ=60)

      GACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCG                                                                                       <  SRR4302534.915928/94‑1 (MQ=60)

        CGCGCAGCGATCTTTTATTCGCTATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAGCCGCCGACGTCGCC                                                                                     <  SRR4302534.6108291/94‑1 (MQ=60)

        CGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCC                                                                                     >  SRR4302534.2782328/1‑94 (MQ=60)

         GCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCG                                                                                    <  SRR4302534.209551/94‑1 (MQ=60)

         GCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCG                                                                                    <  SRR4302534.1252185/94‑1 (MQ=60)

           GCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCA                                                                                  >  SRR4302534.5738316/1‑94 (MQ=60)

            CAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCAT                                                                                 >  SRR4302534.6094189/1‑94 (MQ=60)

            CAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCAT                                                                                 >  SRR4302534.2532106/1‑94 (MQ=60)

            CAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCAT                                                                                 <  SRR4302534.1208158/94‑1 (MQ=60)

             AGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATC                                                                                >  SRR4302534.3916401/1‑94 (MQ=60)

             AGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATC                                                                                >  SRR4302534.3221291/1‑94 (MQ=60)

             AGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATC                                                                                >  SRR4302534.6226486/1‑94 (MQ=60)

             AGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATC                                                                                >  SRR4302534.581792/1‑94 (MQ=60)

              GCGATCTTTTATTCGCGATGGGCTGACGCTTGCTGGCGGTGTGATCGCTAATCTGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCG                                                                               >  SRR4302534.5187711/1‑94 (MQ=60)

              GCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCG                                                                               <  SRR4302534.429642/94‑1 (MQ=60)

               CGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGC                                                                              >  SRR4302534.2721104/1‑94 (MQ=60)

               CGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGC                                                                              <  SRR4302534.6129519/94‑1 (MQ=60)

               CGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGC                                                                              >  SRR4302534.44691/1‑94 (MQ=60)

               CGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGC                                                                              >  SRR4302534.4502224/1‑94 (MQ=60)

                GATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCT                                                                             >  SRR4302534.1103215/1‑94 (MQ=60)

                GATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCT                                                                             >  SRR4302534.2636465/1‑94 (MQ=60)

                GATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCT                                                                             <  SRR4302534.2624429/94‑1 (MQ=60)

                GATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCT                                                                             <  SRR4302534.1752983/94‑1 (MQ=60)

                 ATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTC                                                                            >  SRR4302534.5157265/1‑94 (MQ=60)

                 ATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGCCGCCGCATCGCTC                                                                            >  SRR4302534.5387505/1‑94 (MQ=60)

                  TCTTTTATTCGCGATGGGCTGACGTTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCA                                                                           <  SRR4302534.789113/94‑1 (MQ=60)

                     TTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGC                                                                        >  SRR4302534.6066704/1‑94 (MQ=60)

                     TTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGC                                                                        >  SRR4302534.4576112/1‑94 (MQ=60)

                      TTATTCGCGATTGGCCGACGCTCCCTGTCGTTGTGATCGCTAATCCGAAGATTTTCAGCGTCAAAACCGCCGACGCCGCCTCATCGCTCACGCG                                                                       <  SRR4302534.1340665/94‑1 (MQ=60)

                       TATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGT                                                                      <  SRR4302534.1136999/94‑1 (MQ=60)

                        ATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTA                                                                     <  SRR4302534.5780311/94‑1 (MQ=60)

                         TTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAA                                                                    <  SRR4302534.5861219/94‑1 (MQ=60)

                         TTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAA                                                                    <  SRR4302534.2906016/94‑1 (MQ=60)

                          TCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAAC                                                                   >  SRR4302534.5474845/1‑94 (MQ=60)

                           CGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACT                                                                  >  SRR4302534.864330/1‑94 (MQ=60)

                              GATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGgaagtaca                                                               >  SRR4302534.1536450/1‑86 (MQ=60)

                              GATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACC                                                               <  SRR4302534.4294546/94‑1 (MQ=60)

                               ATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGTAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCA                                                              <  SRR4302534.3293836/94‑1 (MQ=60)

                                TGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAG                                                             <  SRR4302534.5142019/94‑1 (MQ=60)

                                TGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAG                                                             <  SRR4302534.1099808/94‑1 (MQ=60)

                                 GGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGC                                                            <  SRR4302534.4226365/94‑1 (MQ=60)

                                 GGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGC                                                            >  SRR4302534.347214/1‑94 (MQ=60)

                                  GGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCG                                                           <  SRR4302534.3299783/94‑1 (MQ=60)

                                    CTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAA                                                         <  SRR4302534.3824688/94‑1 (MQ=60)

                                    CTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAA                                                         >  SRR4302534.5226693/1‑94 (MQ=60)

                                     TGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACTGCCGACGTCGCCGCATCGCTCACGGGGAACTACCAGCGCAG                                                        >  SRR4302534.5488050/1‑94 (MQ=60)

                                     TGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTAGCAGCGAAG                                                        <  SRR4302534.2356454/94‑1 (MQ=60)

                                     TGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAG                                                        >  SRR4302534.4454983/1‑94 (MQ=60)

                                     TGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAG                                                        >  SRR4302534.532647/1‑94 (MQ=60)

                                     TGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAG                                                        >  SRR4302534.4240992/1‑94 (MQ=60)

                                     TGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCCCTCACGCGTAACTACCAGCGAAG                                                        <  SRR4302534.1633438/94‑1 (MQ=60)

                                      GACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGC                                                       <  SRR4302534.4701850/94‑1 (MQ=60)

                                      GACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGC                                                       <  SRR4302534.4243790/94‑1 (MQ=60)

                                       ACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAGCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCT                                                      <  SRR4302534.3336057/94‑1 (MQ=60)

                                       ACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCT                                                      <  SRR4302534.986894/94‑1 (MQ=60)

                                       ACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCT                                                      <  SRR4302534.486820/94‑1 (MQ=60)

                                       ACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCT                                                      >  SRR4302534.6527858/1‑94 (MQ=60)

                                       ACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCT                                                      >  SRR4302534.5941788/1‑94 (MQ=60)

                                        CGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTA                                                     <  SRR4302534.871598/94‑1 (MQ=60)

                                        CGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTA                                                     <  SRR4302534.3466870/94‑1 (MQ=60)

                                        CGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTA                                                     <  SRR4302534.5753535/94‑1 (MQ=60)

                                         GCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAACCTAT                                                    >  SRR4302534.909087/1‑94 (MQ=60)

                                         GCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGATCACGCGTAACTACCAGCGAAGCTAT                                                    >  SRR4302534.3367938/1‑94 (MQ=60)

                                          CTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATA                                                   >  SRR4302534.3670922/1‑94 (MQ=60)

                                          CTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATA                                                   <  SRR4302534.3376020/94‑1 (MQ=60)

                                          CTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATA                                                   <  SRR4302534.2748157/94‑1 (MQ=60)

                                           TTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATAT                                                  <  SRR4302534.6420984/94‑1 (MQ=60)

                                            TCCTGTCGTTGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAGCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATC                                                 <  SRR4302534.3654565/94‑1 (MQ=60)

                                             CCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCA                                                <  SRR4302534.161612/94‑1 (MQ=60)

                                             CCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCA                                                <  SRR4302534.2677763/94‑1 (MQ=60)

                                               TGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAAT                                              >  SRR4302534.5018556/1‑94 (MQ=60)

                                               TGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAAT                                              <  SRR4302534.4005310/94‑1 (MQ=60)

                                                GTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATA                                             <  SRR4302534.5960265/94‑1 (MQ=60)

                                                GTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATA                                             <  SRR4302534.4152910/94‑1 (MQ=60)

                                                  TGATGTGATCGCTAATCCGCAGTTTTTCAGCGTAAAACCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATT                                           <  SRR4302534.4662611/94‑1 (MQ=60)

                                                   GATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTA                                          >  SRR4302534.3511978/1‑94 (MQ=60)

                                                    ATGTGATCGCTAATCCGCAGATTTTCAGCGACAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAA                                         <  SRR4302534.3539356/94‑1 (MQ=60)

                                                     TGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAAT                                        <  SRR4302534.4227385/94‑1 (MQ=60)

                                                     TGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAAT                                        <  SRR4302534.845948/94‑1 (MQ=60)

                                                      GTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATC                                       <  SRR4302534.355363/94‑1 (MQ=60)

                                                      GTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATC                                       <  SRR4302534.461197/94‑1 (MQ=60)

                                                      GTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATC                                       <  SRR4302534.717629/94‑1 (MQ=60)

                                                      GTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATC                                       <  SRR4302534.659177/94‑1 (MQ=60)

                                                       TGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCG                                      <  SRR4302534.4241446/94‑1 (MQ=60)

                                                        GATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGC                                     >  SRR4302534.5173523/1‑94 (MQ=60)

                                                        GATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGC                                     >  SRR4302534.2361387/1‑94 (MQ=60)

                                                        GATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGC                                     >  SRR4302534.2573221/1‑94 (MQ=60)

                                                         ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCC                                    <  SRR4302534.1925163/94‑1 (MQ=60)

                                                         ATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCC                                    <  SRR4302534.435330/94‑1 (MQ=60)

                                                           TGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCAT                                  >  SRR4302534.3242753/1‑94 (MQ=60)

                                                           CGCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCAT                                  <  SRR4302534.932144/94‑1 (MQ=60)

                                                            GCTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATG                                 <  SRR4302534.1467583/94‑1 (MQ=60)

                                                            GCTAATCCGCAGATTTGCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATG                                 <  SRR4302534.4249246/94‑1 (MQ=60)

                                                             CTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGC                                >  SRR4302534.110992/1‑94 (MQ=60)

                                                             CTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGC                                >  SRR4302534.5991135/1‑94 (MQ=60)

                                                             CTAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGC                                <  SRR4302534.4406042/94‑1 (MQ=60)

                                                              TAATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCT                               >  SRR4302534.3305902/1‑94 (MQ=60)

                                                               AATCCGCAGATTTTCAGCGTCAAACCCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTT                              <  SRR4302534.4093415/94‑1 (MQ=60)

                                                               AATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTT                              <  SRR4302534.1961016/94‑1 (MQ=60)

                                                                ATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTT                             <  SRR4302534.4531868/94‑1 (MQ=60)

                                                                ATCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTT                             >  SRR4302534.3491083/1‑94 (MQ=60)

                                                                 TCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTC                            >  SRR4302534.1039659/1‑94 (MQ=60)

                                                                 TCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTC                            <  SRR4302534.2365074/94‑1 (MQ=60)

                                                                 TCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTC                            <  SRR4302534.5615157/94‑1 (MQ=60)

                                                                 TCCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTC                            <  SRR4302534.4804053/94‑1 (MQ=60)

                                                                  CCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCG                           >  SRR4302534.4552565/1‑94 (MQ=60)

                                                                  CCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCG                           >  SRR4302534.60415/1‑94 (MQ=60)

                                                                  CCGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCG                           >  SRR4302534.5244823/1‑94 (MQ=60)

                                                                   CGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGA                          >  SRR4302534.100280/1‑94 (MQ=60)

                                                                   CGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGA                          >  SRR4302534.5880150/1‑94 (MQ=60)

                                                                   CGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGA                          >  SRR4302534.2025822/1‑94 (MQ=60)

                                                                   CGCAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGA                          >  SRR4302534.1418304/1‑94 (MQ=60)

                                                                     CAGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACT                        <  SRR4302534.985324/94‑1 (MQ=60)

                                                                      AGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTT                       <  SRR4302534.798886/94‑1 (MQ=60)

                                                                      AGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTT                       >  SRR4302534.682862/1‑94 (MQ=60)

                                                                      AGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTT                       <  SRR4302534.6515332/94‑1 (MQ=60)

                                                                      AGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTT                       >  SRR4302534.276312/1‑94 (MQ=60)

                                                                      AGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTT                       >  SRR4302534.3282750/1‑94 (MQ=60)

                                                                      AGATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTT                       >  SRR4302534.5008457/1‑94 (MQ=60)

                                                                       GATTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTG                      <  SRR4302534.5231031/94‑1 (MQ=60)

                                                                         TTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTG                    >  SRR4302534.6077117/1‑94 (MQ=60)

                                                                         TTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTG                    <  SRR4302534.5263055/94‑1 (MQ=60)

                                                                         TTTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTG                    <  SRR4302534.2879622/94‑1 (MQ=60)

                                                                          TTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGG                   <  SRR4302534.2869189/94‑1 (MQ=60)

                                                                          TTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGG                   <  SRR4302534.2030474/94‑1 (MQ=60)

                                                                          TTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGG                   <  SRR4302534.6295931/94‑1 (MQ=60)

                                                                          TTTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGG                   <  SRR4302534.2134624/94‑1 (MQ=60)

                                                                           TTCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGA                  <  SRR4302534.694207/94‑1 (MQ=60)

                                                                            TCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAGCTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGAT                 <  SRR4302534.4025161/94‑1 (MQ=60)

                                                                            TCAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGAT                 <  SRR4302534.4272769/94‑1 (MQ=60)

                                                                             CAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATG                >  SRR4302534.1407616/1‑94 (MQ=60)

                                                                             CAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATG                <  SRR4302534.5768626/94‑1 (MQ=60)

                                                                             CAGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATG                >  SRR4302534.3919884/1‑94 (MQ=60)

                                                                              AGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGA               >  SRR4302534.5327627/1‑94 (MQ=60)

                                                                              AGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGA               >  SRR4302534.4683076/1‑94 (MQ=60)

                                                                              AGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGA               >  SRR4302534.6314804/1‑94 (MQ=60)

                                                                              AGCGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGA               >  SRR4302534.2547840/1‑94 (MQ=60)

                                                                                CGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAA             >  SRR4302534.2940610/1‑94 (MQ=60)

                                                                                CGTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAA             >  SRR4302534.198199/1‑94 (MQ=60)

                                                                                 GTCAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGGCATGCTTTCGACTTGTGGATGAAAT            >  SRR4302534.2530763/1‑94 (MQ=60)

                                                                                   CAAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCG          <  SRR4302534.2730965/94‑1 (MQ=60)

                                                                                    AAAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGC         >  SRR4302534.2808728/1‑94 (MQ=60)

                                                                                     AAACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCT        >  SRR4302534.4049575/1‑94 (MQ=60)

                                                                                      AACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTG       <  SRR4302534.2319102/94‑1 (MQ=60)

                                                                                      AACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTG       <  SRR4302534.3422367/94‑1 (MQ=60)

                                                                                      AACCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTG       <  SRR4302534.6455093/94‑1 (MQ=60)

                                                                                        CCGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGAT     <  SRR4302534.6181281/94‑1 (MQ=60)

                                                                                         CGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATC    <  SRR4302534.3815087/94‑1 (MQ=60)

                                                                                         CGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATC    >  SRR4302534.1600729/1‑94 (MQ=60)

                                                                                         CGCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATC    <  SRR4302534.5352188/94‑1 (MQ=60)

                                                                                          GCCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCC   >  SRR4302534.6154457/1‑94 (MQ=60)

                                                                                           CCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCC  >  SRR4302534.4953928/1‑94 (MQ=60)

GCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCC  >  NC_000913/3867154‑3867338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`