Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,893,936 G→A V101V (GTC→GTT)  yieG ← putative membrane/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,893,9360GA100.0% 1068.4 / NA 271V101V (GTC→GTT) yieGputative membrane/transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (127/144);  total (127/144)

Only 100 of 290 total aligned reads displayed.

GCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGC  >  NC_000913/3893846‑3894025
                                                                                           |                                                                                         
  ggAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTAcaacaacaa                                                                                       >  1:33543/1‑93 (MQ=255)
   gAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACaaa                                                                                      >  2:1904704/1‑93 (MQ=255)
   gAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACaaa                                                                                      >  2:4355985/1‑93 (MQ=255)
    aaaaTCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACaaaa                                                                                     <  2:4338677/93‑1 (MQ=255)
     aaaTCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACaaaaa                                                                                    >  1:6056600/1‑92 (MQ=255)
     aaaTCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAg                                                                                    <  1:1811458/93‑1 (MQ=255)
       aTCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCg                                                                                  >  1:1001698/1‑93 (MQ=255)
         cGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCgaa                                                                                >  1:4379592/1‑93 (MQ=255)
          gTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCgaag                                                                               <  2:759106/93‑1 (MQ=255)
          gTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCgaag                                                                               >  2:6396831/1‑93 (MQ=255)
           tCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCgaaga                                                                              >  1:2273762/1‑93 (MQ=255)
           tCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCgaaga                                                                              >  1:2532157/1‑93 (MQ=255)
             aGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAAc                                                                            <  1:2128688/93‑1 (MQ=255)
             aGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAAc                                                                            <  1:968291/93‑1 (MQ=255)
               tAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGc                                                                          <  2:6162648/93‑1 (MQ=255)
               tAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGc                                                                          >  1:4059176/1‑93 (MQ=255)
               tAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGc                                                                          >  2:2607874/1‑93 (MQ=255)
               tAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGc                                                                          >  2:419605/1‑93 (MQ=255)
                 aCAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCAt                                                                        <  1:3620249/93‑1 (MQ=255)
                 aCAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCAGCCCGACCTGCCACGGCAAGCTCATCGCCTGCACAACAACAAAAGCGAAGAACGCAt                                                                        <  2:1371347/93‑1 (MQ=255)
                  cagcagACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCAtt                                                                       >  2:3578511/1‑93 (MQ=255)
                  cagcagACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCAtt                                                                       <  2:5039017/93‑1 (MQ=255)
                   agcagACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTc                                                                      <  1:4793548/93‑1 (MQ=255)
                    gcagACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCa                                                                     >  2:5325793/1‑93 (MQ=255)
                    gcagACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAAAACATTCa                                                                     >  2:4584327/1‑93 (MQ=38)
                      agACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAgg                                                                   <  2:3270879/93‑1 (MQ=255)
                       gACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGc                                                                  >  1:3164307/1‑93 (MQ=255)
                       gACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGc                                                                  <  2:5532704/93‑1 (MQ=255)
                         ccTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGccc                                                                >  2:3008263/1‑93 (MQ=255)
                         ccTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGccc                                                                <  1:4116747/93‑1 (MQ=255)
                          cTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCa                                                               <  1:3688860/93‑1 (MQ=255)
                          cTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCa                                                               >  2:3852198/1‑93 (MQ=255)
                           tATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCAt                                                              <  2:864620/93‑1 (MQ=255)
                           tATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCAt                                                              >  2:3300208/1‑93 (MQ=255)
                           tATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGCCCCAt                                                              <  1:2387087/93‑1 (MQ=255)
                            aTCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATa                                                             >  2:4707678/1‑93 (MQ=255)
                            aTCGCGCCCCA‑GAAGATTGCGCCCACCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATa                                                             <  1:6373623/93‑1 (MQ=255)
                             tCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAg                                                            <  2:6063487/93‑1 (MQ=255)
                             tCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAg                                                            >  1:2605280/1‑93 (MQ=255)
                              ccgcccccAGGAAGTTTCCGCCCA‑CCCGGCCCGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGc                                                           <  2:6127038/89‑1 (MQ=255)
                               gcgcCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCg                                                          <  1:2805986/93‑1 (MQ=255)
                                   cccA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTg                                                      <  1:1486676/93‑1 (MQ=255)
                                   cccA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTg                                                      <  1:2253315/93‑1 (MQ=255)
                                     cA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGcc                                                    >  1:1725467/1‑93 (MQ=255)
                                     cA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGcc                                                    <  1:804859/93‑1 (MQ=255)
                                     cA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGcc                                                    >  1:1244322/1‑93 (MQ=255)
                                        gaagaTTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAg                                                  >  1:4392768/1‑93 (MQ=255)
                                         aagaTTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGt                                                 <  1:89534/93‑1 (MQ=255)
                                         aagaTTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCACATAGCGGGTGCCAGt                                                 <  2:3546691/93‑1 (MQ=255)
                                             ttGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCaa                                             <  2:3201042/93‑1 (MQ=255)
                                             ttGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCaa                                             >  1:3294140/1‑93 (MQ=255)
                                              tGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAAc                                            <  1:4250154/93‑1 (MQ=255)
                                               gcgcCCATCCCGCCCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACt                                           <  2:34126/93‑1 (MQ=255)
                                               gcgcCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACt                                           <  1:6338755/93‑1 (MQ=255)
                                               gcgcCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACt                                           <  2:5993800/93‑1 (MQ=255)
                                                 gcCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTgg                                         >  1:5422583/1‑93 (MQ=255)
                                                 gcCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTgg                                         >  2:1125209/1‑93 (MQ=255)
                                                  cccATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGc                                        <  1:5821103/93‑1 (MQ=255)
                                                   ccATCCCGACCTGCCACGGCAAGCTCATCGGCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCa                                       >  2:3597314/1‑93 (MQ=255)
                                                   ccATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCa                                       <  2:1605410/93‑1 (MQ=255)
                                                   ccATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCa                                       >  1:3004248/1‑93 (MQ=255)
                                                    cATCCCGCCCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAg                                      <  2:4111311/93‑1 (MQ=255)
                                                    cATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAg                                      <  2:2187900/93‑1 (MQ=255)
                                                     aTCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAgg                                     >  1:5791679/1‑93 (MQ=255)
                                                      tCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGt                                    >  1:1873282/1‑93 (MQ=255)
                                                      tCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGt                                    >  2:5841059/1‑93 (MQ=255)
                                                      tCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGt                                    <  1:4895538/93‑1 (MQ=255)
                                                       cccGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGtt                                   <  2:541162/93‑1 (MQ=255)
                                                        ccGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTa                                  <  1:4001395/93‑1 (MQ=255)
                                                         cGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAg                                 <  2:2286988/93‑1 (MQ=255)
                                                          gACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGc                                >  1:5883062/1‑93 (MQ=255)
                                                            ccTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCaa                              <  1:5773806/93‑1 (MQ=255)
                                                             cTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCaaa                             <  2:5980421/93‑1 (MQ=255)
                                                              tGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAAc                            <  2:4459615/93‑1 (MQ=255)
                                                                ccACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAg                          <  2:5120496/93‑1 (MQ=255)
                                                                  aCGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTc                        <  1:3839380/93‑1 (MQ=255)
                                                                   cGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTcc                       <  1:6478342/93‑1 (MQ=255)
                                                                   cGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTcc                       <  2:5153450/93‑1 (MQ=255)
                                                                   cGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTcc                       <  2:4897475/93‑1 (MQ=255)
                                                                    ggCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTccc                      >  1:5268675/1‑93 (MQ=255)
                                                                    ggCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTccc                      <  2:3330358/93‑1 (MQ=255)
                                                                      cAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCcat                    <  2:6429846/93‑1 (MQ=255)
                                                                       aaGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCcatc                   <  2:2441943/93‑1 (MQ=255)
                                                                       aaGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCcatc                   >  2:3920966/1‑93 (MQ=255)
                                                                       aaGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCcatc                   >  1:2892529/1‑93 (MQ=255)
                                                                       aaGCTCATCGACTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTAGCAAACAGTCCcatc                   >  1:4561931/1‑93 (MQ=255)
                                                                           tcATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCataa               >  1:4185039/2‑93 (MQ=255)
                                                                           tcATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCataa               >  2:1751225/2‑93 (MQ=255)
                                                                           tcATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCataa               >  1:2761495/2‑93 (MQ=255)
                                                                           tcATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCataa               >  1:3364944/2‑93 (MQ=255)
                                                                            cATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCataat              <  1:1055068/93‑1 (MQ=255)
                                                                            cATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTAGCAAACAGTCCCATCataat              >  1:5566384/1‑93 (MQ=255)
                                                                              tCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATAc            >  2:3692845/1‑93 (MQ=255)
                                                                               cGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACt           <  2:2054544/93‑1 (MQ=255)
                                                                                 ccTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGc         <  1:719381/93‑1 (MQ=255)
                                                                                   tGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCg       <  1:5104001/93‑1 (MQ=255)
                                                                                      acaacaacaAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAAt    >  2:1300816/1‑93 (MQ=255)
                                                                                       caacaacaaAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATg   >  2:876293/1‑93 (MQ=255)
                                                                                        aacaacAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGc  <  1:685966/93‑1 (MQ=255)
                                                                                        aacaacAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGc  <  1:2163151/93‑1 (MQ=255)
                                                                                           |                                                                                         
GCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCA‑GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGC  >  NC_000913/3893846‑3894025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG  >  NC_000913/3893843‑3894029
                                                                                             |                                                                                             
AACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACA                                                                                               >  SRR4302534.5080154/1‑94 (MQ=60)
   GCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACA                                                                                            >  SRR4302534.688583/1‑94 (MQ=60)
   GCGGAAAATAGTCAGTAACAGCCGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGACAAGCTCATCGCCTGTACAACAACA                                                                                            <  SRR4302534.4124706/94‑1 (MQ=60)
    CGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAA                                                                                           >  SRR4302534.34421/1‑94 (MQ=60)
    CGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAA                                                                                           >  SRR4302534.533421/1‑94 (MQ=60)
    CGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAA                                                                                           >  SRR4302534.4197545/1‑94 (MQ=60)
      GAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAA                                                                                         >  SRR4302534.827551/1‑94 (MQ=60)
       AAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAA                                                                                        >  SRR4302534.6221300/1‑94 (MQ=60)
        AAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGC                                                                                       <  SRR4302534.2977100/94‑1 (MQ=60)
        AAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGC                                                                                       >  SRR4302534.6535880/1‑94 (MQ=60)
        AAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGC                                                                                       <  SRR4302534.1858503/94‑1 (MQ=60)
         AATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCG                                                                                      >  SRR4302534.1027378/1‑94 (MQ=60)
         AATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCG                                                                                      <  SRR4302534.2857989/94‑1 (MQ=60)
         AATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCG                                                                                      >  SRR4302534.1379217/1‑94 (MQ=60)
           TCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAA                                                                                    >  SRR4302534.4497641/1‑94 (MQ=60)
             GTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCTTCCCGACCTGCCACGGAAAGCTCATCGCCTGTACAACAACAAAAGggagaa                                                                                  >  SRR4302534.5688668/1‑88 (MQ=60)
             GTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGA                                                                                  >  SRR4302534.2599344/1‑94 (MQ=60)
             GTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGA                                                                                  >  SRR4302534.2334074/1‑94 (MQ=60)
              TCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAA                                                                                 >  SRR4302534.3138532/1‑94 (MQ=60)
               CAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAAC                                                                                >  SRR4302534.5210678/1‑94 (MQ=60)
               CAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAAC                                                                                <  SRR4302534.3295120/94‑1 (MQ=60)
                AGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACG                                                                               <  SRR4302534.4324791/94‑1 (MQ=60)
                AGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACG                                                                               <  SRR4302534.992962/94‑1 (MQ=60)
                AGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACG                                                                               <  SRR4302534.3108478/94‑1 (MQ=60)
                AGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACG                                                                               <  SRR4302534.707118/94‑1 (MQ=60)
                AGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACG                                                                               >  SRR4302534.727763/1‑94 (MQ=60)
                AGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACG                                                                               <  SRR4302534.2184762/94‑1 (MQ=60)
                 GTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGC                                                                              >  SRR4302534.4168046/1‑94 (MQ=60)
                  TAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCA                                                                             >  SRR4302534.4263261/1‑94 (MQ=60)
                  TAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCA                                                                             >  SRR4302534.793498/1‑94 (MQ=60)
                  TAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCA                                                                             >  SRR4302534.2902470/1‑94 (MQ=60)
                   AACGGCAGACCTGGCGCGCCCCAGAAGATTGCGCCAATACCGACCTGCCACGGCAAGCTCATCGCCTGTACATCACCAAAAGCGAAGAACGCAT                                                                            <  SRR4302534.3398208/94‑1 (MQ=55)
                    ACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATT                                                                           <  SRR4302534.344967/94‑1 (MQ=60)
                    ACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATT                                                                           >  SRR4302534.4624479/1‑94 (MQ=60)
                    ACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATT                                                                           <  SRR4302534.5381117/94‑1 (MQ=60)
                    ACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATT                                                                           <  SRR4302534.3717572/94‑1 (MQ=60)
                     CAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTC                                                                          >  SRR4302534.1172247/1‑94 (MQ=60)
                      AGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCA                                                                         >  SRR4302534.3988045/1‑94 (MQ=60)
                      AGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCA                                                                         <  SRR4302534.4923701/94‑1 (MQ=60)
                        CAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGG                                                                       <  SRR4302534.200592/94‑1 (MQ=60)
                        CAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGG                                                                       <  SRR4302534.1321722/94‑1 (MQ=60)
                        CAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGG                                                                       >  SRR4302534.5844538/1‑94 (MQ=60)
                        CAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGG                                                                       >  SRR4302534.663421/1‑94 (MQ=60)
                         AGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAACAGCGAAGAAAGCATTCAGGC                                                                      >  SRR4302534.1833580/1‑94 (MQ=60)
                         AGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGC                                                                      >  SRR4302534.3247711/1‑94 (MQ=60)
                         AGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGC                                                                      >  SRR4302534.775511/1‑94 (MQ=60)
                            CCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCA                                                                   <  SRR4302534.4227513/94‑1 (MQ=60)
                            CCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCA                                                                   >  SRR4302534.2230634/1‑94 (MQ=60)
                             CTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCAT                                                                  >  SRR4302534.4817534/1‑94 (MQ=60)
                             CTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCAT                                                                  >  SRR4302534.68685/1‑94 (MQ=60)
                             CTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCAT                                                                  >  SRR4302534.6013851/1‑94 (MQ=60)
                             CTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCAT                                                                  <  SRR4302534.3788045/94‑1 (MQ=60)
                              TATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATA                                                                 >  SRR4302534.4378572/1‑94 (MQ=60)
                              TATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATA                                                                 >  SRR4302534.2318410/1‑94 (MQ=60)
                              TATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGCCCCATA                                                                 <  SRR4302534.2450518/94‑1 (MQ=60)
                               ATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAG                                                                >  SRR4302534.2674216/1‑94 (MQ=60)
                               ATCGCGCCCCAGAAGATTGCGCCCACCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAG                                                                <  SRR4302534.6545714/94‑1 (MQ=60)
                                  GCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGG                                                             <  SRR4302534.2617977/94‑1 (MQ=60)
                                  GCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGG                                                             <  SRR4302534.2879782/94‑1 (MQ=60)
                                   CGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGG                                                            >  SRR4302534.4338420/1‑94 (MQ=60)
                                    GCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGT                                                           <  SRR4302534.2297516/94‑1 (MQ=60)
                                      CCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGC                                                         <  SRR4302534.2313045/94‑1 (MQ=60)
                                      CCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGC                                                         <  SRR4302534.1525787/94‑1 (MQ=60)
                                       CCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCC                                                        >  SRR4302534.1770274/1‑94 (MQ=60)
                                       CCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCC                                                        >  SRR4302534.6285087/1‑94 (MQ=60)
                                       CCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCC                                                        >  SRR4302534.1277117/1‑94 (MQ=60)
                                        CAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCA                                                       <  SRR4302534.1723523/94‑1 (MQ=60)
                                        CAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCA                                                       <  SRR4302534.825080/94‑1 (MQ=60)
                                         AGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAG                                                      >  SRR4302534.4511238/1‑94 (MQ=60)
                                         AGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAG                                                      <  SRR4302534.5578020/94‑1 (MQ=60)
                                          GAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGT                                                     <  SRR4302534.3658551/94‑1 (MQ=60)
                                           AAGATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTG                                                    <  SRR4302534.91921/94‑1 (MQ=60)
                                             GATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCA                                                  >  SRR4302534.3956943/1‑94 (MQ=60)
                                              ATTGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAA                                                 >  SRR4302534.3382006/1‑94 (MQ=60)
                                                TGCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACT                                               <  SRR4302534.4364812/94‑1 (MQ=60)
                                                 GCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTG                                              <  SRR4302534.6509976/94‑1 (MQ=60)
                                                 GCGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTG                                              <  SRR4302534.3400033/94‑1 (MQ=60)
                                                  CGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGG                                             >  SRR4302534.5568511/1‑94 (MQ=60)
                                                  CGCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGG                                             >  SRR4302534.659706/1‑94 (MQ=60)
                                                   GCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGC                                            >  SRR4302534.4961669/1‑94 (MQ=60)
                                                   GCCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGC                                            >  SRR4302534.554556/1‑94 (MQ=60)
                                                    CCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCA                                           <  SRR4302534.3296046/94‑1 (MQ=60)
                                                    CCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCA                                           >  SRR4302534.3082839/1‑94 (MQ=60)
                                                    CCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCA                                           <  SRR4302534.4224594/94‑1 (MQ=60)
                                                    CCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCA                                           <  SRR4302534.5979104/94‑1 (MQ=60)
                                                    CCCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCA                                           >  SRR4302534.378116/1‑94 (MQ=60)
                                                     CCATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAG                                          <  SRR4302534.2805150/94‑1 (MQ=60)
                                                      CATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGG                                         >  SRR4302534.5948810/1‑94 (MQ=60)
                                                       ATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGT                                        >  SRR4302534.1922034/1‑94 (MQ=60)
                                                       ATCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGT                                        >  SRR4302534.6266960/1‑94 (MQ=60)
                                                        TCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTT                                       <  SRR4302534.5028263/94‑1 (MQ=60)
                                                        TCCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTT                                       <  SRR4302534.2186344/94‑1 (MQ=60)
                                                         CCCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTA                                      <  SRR4302534.4378258/94‑1 (MQ=60)
                                                          CCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAG                                     >  SRR4302534.1806348/1‑94 (MQ=60)
                                                          CCGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAG                                     <  SRR4302534.4108520/94‑1 (MQ=60)
                                                           CGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGC                                    >  SRR4302534.6042805/1‑94 (MQ=60)
                                                           CGACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGC                                    >  SRR4302534.5102795/1‑94 (MQ=60)
                                                            GACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCA                                   >  SRR4302534.4789367/1‑94 (MQ=60)
                                                            GACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCA                                   >  SRR4302534.3001764/1‑94 (MQ=60)
                                                            GACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCA                                   >  SRR4302534.2561507/1‑94 (MQ=60)
                                                            GACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCA                                   >  SRR4302534.1913017/1‑94 (MQ=60)
                                                            GACCTGCCACGGCAAGCTCAGCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCA                                   <  SRR4302534.2714602/94‑1 (MQ=60)
                                                             ACCTGCCGCGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAA                                  <  SRR4302534.456229/94‑1 (MQ=60)
                                                             ACCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTAGCAA                                  >  SRR4302534.969932/1‑94 (MQ=60)
                                                              CCTGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAA                                 <  SRR4302534.5930439/94‑1 (MQ=60)
                                                                TGCCACGGCAAGCTCATCGCCTGTACAACAGCAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACA                               <  SRR4302534.361003/94‑1 (MQ=60)
                                                                TGCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGGCCGTGGAACTGGCAGGTTAGCAAACA                               >  SRR4302534.6171824/1‑94 (MQ=60)
                                                                 GCCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAG                              <  SRR4302534.3739449/94‑1 (MQ=60)
                                                                  CCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGT                             >  SRR4302534.6090907/1‑94 (MQ=60)
                                                                  CCACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGT                             <  SRR4302534.5931101/94‑1 (MQ=60)
                                                                   CACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTC                            <  SRR4302534.4504811/94‑1 (MQ=60)
                                                                    CCGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCC                           <  SRR4302534.875343/94‑1 (MQ=60)
                                                                    ACGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCC                           <  SRR4302534.3942504/94‑1 (MQ=60)
                                                                     CGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCC                          <  SRR4302534.6652939/94‑1 (MQ=60)
                                                                     CGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCC                          <  SRR4302534.2917576/94‑1 (MQ=60)
                                                                     CGGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCC                          >  SRR4302534.5410997/1‑94 (MQ=60)
                                                                      GGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCA                         <  SRR4302534.1091675/94‑1 (MQ=60)
                                                                      GGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCA                         >  SRR4302534.5940358/1‑94 (MQ=60)
                                                                      GGCAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCA                         <  SRR4302534.4927476/94‑1 (MQ=60)
                                                                        CAAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATC                       >  SRR4302534.2968439/1‑94 (MQ=60)
                                                                        CAAGCTCATCGACTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTAGCAAACAGTCCCATC                       >  SRR4302534.4685611/1‑94 (MQ=60)
                                                                         AAGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCA                      >  SRR4302534.5718334/1‑94 (MQ=60)
                                                                          AGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCAT                     >  SRR4302534.2213202/1‑94 (MQ=60)
                                                                          AGCTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCAT                     <  SRR4302534.2013494/94‑1 (MQ=60)
                                                                            CTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAA                   >  SRR4302534.4165581/1‑94 (MQ=60)
                                                                            CTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAA                   <  SRR4302534.5962760/94‑1 (MQ=60)
                                                                            CTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAA                   >  SRR4302534.3455072/1‑94 (MQ=60)
                                                                            CTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAA                   >  SRR4302534.4298142/1‑94 (MQ=60)
                                                                            CTCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAA                   >  SRR4302534.2834224/1‑94 (MQ=60)
                                                                             TCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAAT                  >  SRR4302534.2358360/1‑94 (MQ=60)
                                                                             TCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAAT                  >  SRR4302534.2834296/1‑94 (MQ=60)
                                                                             TCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAAT                  >  SRR4302534.5494421/1‑94 (MQ=60)
                                                                             TCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAAT                  <  SRR4302534.2523226/94‑1 (MQ=60)
                                                                             TCATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTAGCAAACAGTCCCATCATAAT                  >  SRR4302534.5716707/1‑94 (MQ=60)
                                                                              CATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATA                 >  SRR4302534.1063919/1‑94 (MQ=60)
                                                                              CATCGCCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATA                 <  SRR4302534.1082380/94‑1 (MQ=60)
                                                                                   CCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCC            <  SRR4302534.737123/94‑1 (MQ=60)
                                                                                   CCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCC            <  SRR4302534.3790177/94‑1 (MQ=60)
                                                                                   CCTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCC            <  SRR4302534.190698/94‑1 (MQ=60)
                                                                                    CTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCG           <  SRR4302534.3851376/94‑1 (MQ=60)
                                                                                    CTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCG           <  SRR4302534.2659541/94‑1 (MQ=60)
                                                                                    CTGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCG           <  SRR4302534.1529180/94‑1 (MQ=60)
                                                                                     TGTACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGA          <  SRR4302534.5241754/94‑1 (MQ=60)
                                                                                       TACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAAT        <  SRR4302534.6016882/94‑1 (MQ=60)
                                                                                        ACAACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATG       <  SRR4302534.2316058/94‑1 (MQ=60)
                                                                                          AACAACAAAAGCGAAGAACGCATTCAGGCGCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCA     <  SRR4302534.4263012/94‑1 (MQ=60)
                                                                                          AACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAGCTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCAAATGCA     <  SRR4302534.6544786/94‑1 (MQ=60)
                                                                                          AACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGCATGCA     >  SRR4302534.626248/1‑94 (MQ=60)
                                                                                          AACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCA     <  SRR4302534.702728/94‑1 (MQ=60)
                                                                                          AACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCA     >  SRR4302534.4303416/1‑94 (MQ=60)
                                                                                          AACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCA     <  SRR4302534.2220284/94‑1 (MQ=60)
                                                                                           ACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAG    <  SRR4302534.3070843/94‑1 (MQ=60)
                                                                                           ACAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAG    <  SRR4302534.3014405/94‑1 (MQ=60)
                                                                                            CAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGC   >  SRR4302534.6039161/1‑94 (MQ=60)
                                                                                            CAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGC   <  SRR4302534.4545405/94‑1 (MQ=60)
                                                                                            CAACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGC   >  SRR4302534.420625/1‑94 (MQ=60)
                                                                                             AACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG  <  SRR4302534.4426981/94‑1 (MQ=60)
                                                                                             |                                                                                             
AACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG  >  NC_000913/3893843‑3894029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑