Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,929,523 T→C V194A (GTG→GCG)  kup → low affinity potassium transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,929,5230TC100.0% 686.2 / NA 198V194A (GTG→GCG) kuplow affinity potassium transport system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (91/107);  total (91/107)

Only 100 of 210 total aligned reads displayed.

TGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGT  >  NC_000913/3929433‑3929612
                                                                                          |                                                                                         
tGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAgcgc                                                                                         >  1:2048357/1‑93 (MQ=255)
tGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAATCCGGAAgcgc                                                                                         >  1:3776768/1‑93 (MQ=255)
 ggTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGcgct                                                                                        >  1:426332/1‑93 (MQ=255)
   tCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGcgctgc                                                                                      <  2:3572291/93‑1 (MQ=255)
    cGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCa                                                                                     <  1:4444825/93‑1 (MQ=255)
     ggTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCAt                                                                                    >  1:5846092/1‑93 (MQ=255)
      gTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATg                                                                                   <  2:4031413/93‑1 (MQ=255)
      gTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATg                                                                                   <  2:240338/93‑1 (MQ=255)
        aaGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATgcg                                                                                 >  1:3380188/1‑93 (MQ=255)
          gCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCt                                                                               >  2:1282614/1‑93 (MQ=255)
          gCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCt                                                                               >  1:1815625/1‑93 (MQ=255)
             gTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGaa                                                                            <  2:5649806/93‑1 (MQ=255)
             gTTTCCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGaa                                                                            <  1:1939469/93‑1 (MQ=255)
              tttGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAAt                                                                           >  2:4743877/1‑93 (MQ=255)
               ttGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATc                                                                          <  2:3948945/93‑1 (MQ=255)
                tGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATcc                                                                         <  2:4874073/93‑1 (MQ=255)
                  cgcCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCaa                                                                       >  1:4661502/1‑93 (MQ=255)
                    ccGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAAtg                                                                     <  1:2563004/93‑1 (MQ=255)
                       aTCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTgg                                                                  <  2:4665034/93‑1 (MQ=255)
                       aTCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTgg                                                                  >  2:2983764/1‑93 (MQ=255)
                        tCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTggg                                                                 <  2:1097685/93‑1 (MQ=255)
                           tGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCgg                                                              <  2:320439/93‑1 (MQ=255)
                             cTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTg                                                            >  1:1560698/1‑93 (MQ=255)
                                aCCTGGTTTTTGATTCTGGCAGGACTGGGGTTTCGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAt                                                         >  2:50953/1‑93 (MQ=255)
                                aCCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAt                                                         >  2:1427402/1‑93 (MQ=255)
                                   tGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttc                                                      >  1:717669/1‑93 (MQ=255)
                                    ggTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttct                                                     <  2:1880697/93‑1 (MQ=255)
                                     gTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttctt                                                    >  2:4727131/1‑93 (MQ=255)
                                     gTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttctt                                                    <  2:2009225/93‑1 (MQ=255)
                                      tttttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGACTCCAATGTGGGCGGCGCATttcttc                                                   >  2:4146803/1‑93 (MQ=255)
                                       ttttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTcc                                                  <  1:4938465/93‑1 (MQ=255)
                                         ttGATTCTGGCAGGACTGGGGTTACGTATCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCtt                                                <  1:4014307/93‑1 (MQ=255)
                                          tGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTg                                               <  1:1458410/93‑1 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                              <  2:3598511/93‑1 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                              <  1:129598/93‑1 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                              >  1:2581863/1‑93 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                              >  2:5905944/1‑93 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                              >  1:2362705/1‑93 (MQ=255)
                                            aTTCTGGCAGGACTGGGGTTACGTAGCATTATTACTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGaa                                             >  2:6305184/1‑93 (MQ=255)
                                             ttCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAAt                                            >  2:3533646/1‑93 (MQ=255)
                                             ttCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAAt                                            <  1:5602699/93‑1 (MQ=255)
                                              tCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATa                                           <  1:3753192/93‑1 (MQ=255)
                                              tCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATa                                           <  2:2471988/93‑1 (MQ=255)
                                                tGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACa                                         >  2:1177200/1‑93 (MQ=255)
                                                 ggCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaa                                        <  1:3231967/93‑1 (MQ=255)
                                                  gCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaaa                                       >  2:2590615/1‑93 (MQ=255)
                                                    aGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                     >  1:2259914/1‑93 (MQ=255)
                                                    aGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                     <  1:2894521/93‑1 (MQ=255)
                                                     ggACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACg                                    >  2:3149300/1‑93 (MQ=255)
                                                     ggACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACg                                    <  1:5463544/93‑1 (MQ=255)
                                                      gACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACgg                                   >  2:734022/1‑93 (MQ=255)
                                                      gACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACgg                                   <  1:3665202/93‑1 (MQ=255)
                                                       aCTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGt                                  <  2:3324744/93‑1 (MQ=255)
                                                       aCTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGt                                  >  2:2809093/1‑93 (MQ=255)
                                                       aCTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGt                                  <  2:2721400/93‑1 (MQ=255)
                                                        cTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGtt                                 <  1:6462153/93‑1 (MQ=255)
                                                         tGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGttt                                >  1:4809833/1‑93 (MQ=255)
                                                          ggggTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTc                               <  1:6364396/93‑1 (MQ=255)
                                                            ggTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCtt                             <  2:1333429/93‑1 (MQ=255)
                                                            ggTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCtt                             <  1:5314535/93‑1 (MQ=255)
                                                             gTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                            <  1:98456/93‑1 (MQ=255)
                                                             gTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                            >  1:5009297/1‑93 (MQ=255)
                                                             gTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                            <  2:1479364/93‑1 (MQ=255)
                                                             gTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                            <  2:4205053/93‑1 (MQ=255)
                                                               tACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTa                          <  2:1948404/93‑1 (MQ=255)
                                                               tACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTa                          <  2:3650163/93‑1 (MQ=255)
                                                               tACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTa                          <  1:2183882/93‑1 (MQ=255)
                                                                aCGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAt                         >  2:2135401/1‑93 (MQ=255)
                                                                aCGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAt                         <  2:5261703/93‑1 (MQ=255)
                                                                  gTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTg                       >  1:880784/1‑93 (MQ=255)
                                                                   tAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGc                      <  1:5777568/93‑1 (MQ=255)
                                                                    aGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCa                     >  2:1722571/1‑93 (MQ=255)
                                                                     gCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAt                    <  2:2804838/93‑1 (MQ=255)
                                                                     gCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAt                    >  1:5972275/1‑93 (MQ=255)
                                                                     gCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAt                    >  1:248751/1‑93 (MQ=255)
                                                                        ttattGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAg                 <  2:4310804/93‑1 (MQ=255)
                                                                         tattGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgg                <  2:865564/93‑1 (MQ=255)
                                                                          attGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACCAAACGGTTTCTTTTATTGCATTAggg               >  1:2646845/1‑93 (MQ=255)
                                                                          attGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTGCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAggg               <  1:2803542/93‑1 (MQ=255)
                                                                           ttGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgggg              >  1:661904/1‑93 (MQ=255)
                                                                              cTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAg           >  1:1707312/1‑93 (MQ=255)
                                                                               tAACGCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgt          <  1:1187065/93‑1 (MQ=255)
                                                                               tAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgt          >  2:1788239/1‑93 (MQ=255)
                                                                               tAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgg          >  2:3403275/1‑92 (MQ=255)
                                                                                aaCCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtg         >  2:6265022/1‑93 (MQ=255)
                                                                                 aCCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtgg        <  2:5690596/93‑1 (MQ=255)
                                                                                 aCCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtgg        <  1:3838295/93‑1 (MQ=255)
                                                                                  cccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggt       <  1:6153929/93‑1 (MQ=255)
                                                                                  cccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggt       >  2:6025657/1‑93 (MQ=255)
                                                                                  cccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGCGGCAgtggt       >  1:1022355/1‑93 (MQ=255)
                                                                                   ccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggtg      <  1:4078774/93‑1 (MQ=255)
                                                                                   ccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggtg      <  1:6125073/93‑1 (MQ=255)
                                                                                    cGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGgtgt     <  1:2887423/93‑1 (MQ=255)
                                                                                    cGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGgtgt     <  1:2400196/93‑1 (MQ=255)
                                                                                    cGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGgtgt     >  1:5061615/1‑93 (MQ=255)
                                                                                     ggAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgtt    <  2:4995913/93‑1 (MQ=255)
                                                                                      gAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgttg   >  1:6454953/1‑93 (MQ=255)
                                                                                      gAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgttg   >  2:3049477/1‑93 (MQ=255)
                                                                                      gAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCCTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgttg   >  1:5356432/1‑93 (MQ=255)
                                                                                       aaGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgttgt  >  1:4329134/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGT  >  NC_000913/3929433‑3929612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGAT  >  NC_000913/3929430‑3929616
                                                                                             |                                                                                             
CTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGC                                                                                               >  SRR4302534.4114465/1‑94 (MQ=60)
 TATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCG                                                                                              >  SRR4302534.6008964/1‑94 (MQ=60)
 TATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCG                                                                                              <  SRR4302534.1848841/94‑1 (MQ=60)
  ATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGC                                                                                             >  SRR4302534.4278465/1‑94 (MQ=60)
  ATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGC                                                                                             >  SRR4302534.2101895/1‑94 (MQ=60)
  ATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAATCCGGAAGCGC                                                                                             >  SRR4302534.3878163/1‑94 (MQ=60)
   TGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCT                                                                                            <  SRR4302534.5534712/94‑1 (MQ=60)
   TGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCT                                                                                            >  SRR4302534.437214/1‑94 (MQ=60)
    GGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTG                                                                                           >  SRR4302534.4137738/1‑94 (MQ=60)
    GGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTG                                                                                           <  SRR4302534.3173783/94‑1 (MQ=60)
       CGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCAT                                                                                        >  SRR4302534.6004812/1‑94 (MQ=60)
       CGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCAT                                                                                        <  SRR4302534.4564918/94‑1 (MQ=60)
          TAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCG                                                                                     >  SRR4302534.3470799/1‑94 (MQ=60)
            AGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCT                                                                                   >  SRR4302534.1862804/1‑94 (MQ=60)
            AGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCT                                                                                   >  SRR4302534.6313939/1‑94 (MQ=60)
                GTTTCCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAAT                                                                               <  SRR4302534.1990183/94‑1 (MQ=60)
                    GCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAA                                                                           >  SRR4302534.4788059/1‑94 (MQ=60)
                       CCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGT                                                                        <  SRR4302534.2630929/94‑1 (MQ=60)
                         GATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTTGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGG                                                                      >  SRR4302534.5498021/1‑94 (MQ=60)
                         GATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGG                                                                      >  SRR4302534.439651/1‑94 (MQ=60)
                             ATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGG                                                                  >  SRR4302534.4449337/1‑94 (MQ=60)
                               GCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTG                                                                <  SRR4302534.6596962/94‑1 (MQ=60)
                               GCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTG                                                                >  SRR4302534.1601552/1‑94 (MQ=60)
                                 TGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGTATCCAATGTGGGCGGTGCA                                                              >  SRR4302534.2644690/1‑94 (MQ=60)
                                 TGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCA                                                              >  SRR4302534.5957435/1‑94 (MQ=60)
                                  GACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAT                                                             >  SRR4302534.6284994/1‑94 (MQ=60)
                                  GACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAT                                                             >  SRR4302534.2772948/1‑94 (MQ=60)
                                   ACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATT                                                            <  SRR4302534.2622398/94‑1 (MQ=60)
                                   ACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATT                                                            >  SRR4302534.6431059/1‑94 (MQ=60)
                                     CTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTC                                                          >  SRR4302534.1296724/1‑94 (MQ=60)
                                     CTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTC                                                          >  SRR4302534.735364/1‑94 (MQ=60)
                                          TTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCT                                                     <  SRR4302534.5072276/94‑1 (MQ=60)
                                           TTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTT                                                    <  SRR4302534.1985132/94‑1 (MQ=60)
                                           TTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTT                                                    <  SRR4302534.2098422/94‑1 (MQ=60)
                                            TTGATTCTGGCAGGACTGGGGTTACGTATCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTG                                                   <  SRR4302534.4121868/94‑1 (MQ=60)
                                             TGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGA                                                  <  SRR4302534.3932884/94‑1 (MQ=60)
                                             TGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGA                                                  >  SRR4302534.2650230/1‑94 (MQ=60)
                                             TGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGA                                                  <  SRR4302534.4573174/94‑1 (MQ=60)
                                             TGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGA                                                  >  SRR4302534.2425421/1‑94 (MQ=60)
                                             TGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGA                                                  <  SRR4302534.4546656/94‑1 (MQ=60)
                                             TGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGA                                                  <  SRR4302534.1496766/94‑1 (MQ=60)
                                              GATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAA                                                 <  SRR4302534.133023/94‑1 (MQ=60)
                                                TTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATA                                               <  SRR4302534.5754219/94‑1 (MQ=60)
                                                TTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATA                                               >  SRR4302534.1109667/1‑94 (MQ=60)
                                                 TCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATAC                                              <  SRR4302534.5103830/94‑1 (MQ=60)
                                                 TCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATAC                                              <  SRR4302534.3853984/94‑1 (MQ=60)
                                                 TCTAGCAGGACTGGGGTTACGTAGCATTATTGCTAACCAGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATAC                                              <  SRR4302534.5704908/94‑1 (MQ=60)
                                                   TGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAA                                            <  SRR4302534.514722/94‑1 (MQ=60)
                                                    GGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAA                                           <  SRR4302534.3317875/94‑1 (MQ=60)
                                                    GGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAA                                           <  SRR4302534.531190/94‑1 (MQ=60)
                                                      CAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAC                                         >  SRR4302534.2319829/1‑94 (MQ=60)
                                                       AGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACG                                        <  SRR4302534.2970500/94‑1 (MQ=60)
                                                        GGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGG                                       <  SRR4302534.5610805/94‑1 (MQ=60)
                                                         GACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGT                                      <  SRR4302534.3763701/94‑1 (MQ=60)
                                                          ACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTT                                     <  SRR4302534.4012772/94‑1 (MQ=60)
                                                          ACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTT                                     <  SRR4302534.1812113/94‑1 (MQ=60)
                                                          ACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTT                                     >  SRR4302534.3863684/1‑94 (MQ=60)
                                                           CTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTT                                    <  SRR4302534.6636208/94‑1 (MQ=60)
                                                           CTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTT                                    >  SRR4302534.4940404/1‑94 (MQ=60)
                                                             GGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCT                                  <  SRR4302534.6536246/94‑1 (MQ=60)
                                                               GGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTT                                <  SRR4302534.5457912/94‑1 (MQ=60)
                                                               GGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTT                                >  SRR4302534.5144693/1‑94 (MQ=60)
                                                               GATTACGTCGCATAAATGCTAGCCCGGAAGCGCTACATGCACTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACAGTTCCTTT                                <  SRR4302534.6652910/94‑1 (MQ=42)
                                                                GTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTT                               <  SRR4302534.101085/94‑1 (MQ=60)
                                                                GTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTT                               <  SRR4302534.2184972/94‑1 (MQ=60)
                                                                 TTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTA                              >  SRR4302534.871780/1‑94 (MQ=60)
                                                                 TTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTA                              <  SRR4302534.4857181/94‑1 (MQ=60)
                                                                 TTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTA                              >  SRR4302534.2377581/1‑94 (MQ=60)
                                                                  TACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAT                             >  SRR4302534.142536/1‑94 (MQ=60)
                                                                  TACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAT                             <  SRR4302534.4995727/94‑1 (MQ=60)
                                                                  TACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAT                             <  SRR4302534.2241615/94‑1 (MQ=60)
                                                                   ACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGCTTCTTTTATT                            >  SRR4302534.2438819/1‑94 (MQ=60)
                                                                    CGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTG                           <  SRR4302534.6096221/94‑1 (MQ=60)
                                                                    CGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTG                           <  SRR4302534.3601707/94‑1 (MQ=60)
                                                                    CGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTG                           >  SRR4302534.903059/1‑94 (MQ=60)
                                                                    CGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTG                           <  SRR4302534.5482110/94‑1 (MQ=60)
                                                                    CGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTG                           <  SRR4302534.1543272/94‑1 (MQ=60)
                                                                      TAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCA                         <  SRR4302534.5934290/94‑1 (MQ=60)
                                                                       AGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAT                        >  SRR4302534.6134480/1‑94 (MQ=60)
                                                                       AGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAT                        <  SRR4302534.2260067/94‑1 (MQ=60)
                                                                       AGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAT                        >  SRR4302534.255240/1‑94 (MQ=60)
                                                                       AGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAT                        >  SRR4302534.3102539/1‑94 (MQ=60)
                                                                        GCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATT                       <  SRR4302534.2964002/94‑1 (MQ=60)
                                                                          ATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAG                     <  SRR4302534.2310693/94‑1 (MQ=60)
                                                                           TTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGG                    <  SRR4302534.2687837/94‑1 (MQ=60)
                                                                            TATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACCAAACGGTTTCTTTTATTGCATTAGGG                   >  SRR4302534.2716833/1‑94 (MQ=60)
                                                                            TATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGG                   <  SRR4302534.6629403/94‑1 (MQ=60)
                                                                             ATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGG                  >  SRR4302534.678000/1‑94 (MQ=60)
                                                                             ATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGG                  >  SRR4302534.978680/1‑94 (MQ=60)
                                                                             ATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGG                  <  SRR4302534.1640506/94‑1 (MQ=60)
                                                                             ATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTGCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGG                  <  SRR4302534.2877288/94‑1 (MQ=60)
                                                                             AGTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGG                  <  SRR4302534.85995/94‑1 (MQ=60)
                                                                              TTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGC                 <  SRR4302534.4508029/94‑1 (MQ=60)
                                                                               TGCTATCACGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGTTGCATTTCTTCCTTGAATACAAAACGGTTGCTTTTATTGCATTAGGGGCA                <  SRR4302534.3831648/94‑1 (MQ=60)
                                                                                GCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG               >  SRR4302534.1751682/1‑94 (MQ=60)
                                                                                GCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG               >  SRR4302534.3351764/1‑94 (MQ=60)
                                                                                  TAACGCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTG             <  SRR4302534.1218278/94‑1 (MQ=60)
                                                                                    ACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGT           <  SRR4302534.3941390/94‑1 (MQ=60)
                                                                                    ACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGG           >  SRR4302534.711139/1‑94 (MQ=60)
                                                                                    ACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGCGGCAGTGGT           >  SRR4302534.1048679/1‑94 (MQ=60)
                                                                                     CCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTG          <  SRR4302534.6321337/94‑1 (MQ=60)
                                                                                     CCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTG          >  SRR4302534.2465876/1‑94 (MQ=60)
                                                                                      CCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGT         >  SRR4302534.5198303/1‑94 (MQ=60)
                                                                                      CCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGT         >  SRR4302534.5852841/1‑94 (MQ=60)
                                                                                      CCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGT         <  SRR4302534.2885471/94‑1 (MQ=60)
                                                                                      CCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGT         <  SRR4302534.6291679/94‑1 (MQ=60)
                                                                                      CCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGT         <  SRR4302534.4188247/94‑1 (MQ=60)
                                                                                       CGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTT        <  SRR4302534.2463969/94‑1 (MQ=60)
                                                                                       CGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTT        >  SRR4302534.5821069/1‑94 (MQ=60)
                                                                                       CGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTT        <  SRR4302534.2963188/94‑1 (MQ=60)
                                                                                        GGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTG       >  SRR4302534.6628879/1‑94 (MQ=60)
                                                                                        GGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCCTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTG       >  SRR4302534.5500828/1‑94 (MQ=60)
                                                                                         GAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGT      >  SRR4302534.4446222/1‑94 (MQ=60)
                                                                                          AAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTC     >  SRR4302534.4590436/1‑94 (MQ=60)
                                                                                           AGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCG    <  SRR4302534.3848633/94‑1 (MQ=60)
                                                                                           AGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCG    >  SRR4302534.712042/1‑94 (MQ=60)
                                                                                           AGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCG    <  SRR4302534.3315646/94‑1 (MQ=60)
                                                                                           AGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAATGCATTAGGGGCAGTGGTGTTGTCG    >  SRR4302534.5765440/1‑94 (MQ=60)
                                                                                           AGCGCTGCATGCGCTGAATCCAATGTGGGCGGTACATTTCTTCATTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTttgg    <  SRR4302534.2676404/94‑5 (MQ=60)
                                                                                             CGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGAT  <  SRR4302534.2341537/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGAT  >  NC_000913/3929430‑3929616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑