Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,936,289	A→G	D146G (GAC→GGC)	rbsR →	RbsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,936,289	0	A	G	99.7%	1000.4 / ‑4.7	288	D146G (GAC→GGC)	rbsR	RbsR
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (124/163); total (124/164)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

Only 100 of 302 total aligned reads displayed.

TTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAG  >  NC_000913/3936199‑3936378

ttGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCt                                                                                         >  2:2669077/1‑93 (MQ=255)

ttGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCt                                                                                         <  1:2757685/93‑1 (MQ=255)

 tGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTg                                                                                        >  2:641814/1‑93 (MQ=255)

  gATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTgg                                                                                       >  1:5200275/1‑93 (MQ=255)

  gATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTgg                                                                                       >  1:1910528/1‑93 (MQ=255)

  gATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTgg                                                                                       >  2:2871526/1‑93 (MQ=255)

  gATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTgg                                                                                       >  1:225655/1‑93 (MQ=255)

   aTGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTggg                                                                                      <  2:5885014/93‑1 (MQ=255)

    tGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGAtgggctgggc                                                                                     <  2:4818757/93‑1 (MQ=255)

      gCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTc                                                                                   <  1:3454069/93‑1 (MQ=255)

         tGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGt                                                                                <  1:5857491/93‑1 (MQ=255)

         tGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGt                                                                                <  2:1199447/93‑1 (MQ=255)

          gCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGtt                                                                               <  2:1799056/93‑1 (MQ=255)

          gCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGtt                                                                               <  2:3375438/93‑1 (MQ=255)

           cTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTc                                                                              <  2:5536796/93‑1 (MQ=255)

            tGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCg                                                                             >  2:127205/1‑93 (MQ=255)

             gTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGa                                                                            >  1:849095/1‑93 (MQ=255)

              ttACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGAt                                                                           <  2:5999102/93‑1 (MQ=255)

               tACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATg                                                                          >  2:2131062/1‑93 (MQ=255)

                aCTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATgg                                                                         >  1:2695189/1‑93 (MQ=255)

                aCTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATgg                                                                         >  1:216123/1‑93 (MQ=255)

                 cTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGc                                                                        >  2:5390312/1‑93 (MQ=255)

                  tgtgCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCg                                                                       <  2:4424387/93‑1 (MQ=255)

                   gtgCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGa                                                                      <  2:374402/93‑1 (MQ=255)

                     gCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACa                                                                    <  2:389368/93‑1 (MQ=255)

                      cACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAg                                                                   <  2:1315785/93‑1 (MQ=255)

                         cGAACCGCATCAGCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGa                                                                <  2:5150992/93‑1 (MQ=255)

                         cGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGa                                                                <  2:2494142/93‑1 (MQ=255)

                         cGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGa                                                                <  1:3588843/93‑1 (MQ=255)

                         cGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGa                                                                <  2:859937/93‑1 (MQ=255)

                          gAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGAt                                                               <  1:2103672/93‑1 (MQ=255)

                          gAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGAt                                                               >  1:3033162/1‑93 (MQ=255)

                          gAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGAt                                                               >  1:5385180/1‑93 (MQ=255)

                           aaaCGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATc                                                              <  2:5179562/93‑1 (MQ=255)

                            aaCGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCt                                                             <  2:364955/93‑1 (MQ=255)

                            aaCGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCt                                                             <  2:2806660/93‑1 (MQ=255)

                            aaCGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCt                                                             >  1:1172643/1‑93 (MQ=255)

                             aCGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCtt                                                            <  1:1775283/93‑1 (MQ=255)

                             aCGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCtt                                                            <  2:3271916/93‑1 (MQ=255)

                                cATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTAtt                                                         >  1:6281415/1‑93 (MQ=255)

                                cATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTAtt                                                         <  1:4645943/93‑1 (MQ=255)

                                 aTCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTc                                                        <  1:1364411/93‑1 (MQ=255)

                                  tCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCa                                                       <  2:2894150/93‑1 (MQ=255)

                                  tCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCa                                                       <  2:1576143/93‑1 (MQ=255)

                                   cAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAg                                                      <  2:2386877/93‑1 (MQ=255)

                                    aaCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAgg                                                     >  2:5081409/1‑93 (MQ=255)

                                    aaCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAgg                                                     >  2:5634061/1‑93 (MQ=255)

                                    aaCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAgg                                                     <  2:1739106/93‑1 (MQ=255)

                                     aCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGa                                                    <  1:2808106/93‑1 (MQ=255)

                                     aCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGa                                                    >  2:1980231/1‑93 (MQ=255)

                                     aCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGa                                                    <  1:703066/93‑1 (MQ=255)

                                      ccTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGAt                                                   >  1:5824701/1‑93 (MQ=255)

                                      ccTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGAt                                                   >  2:6364319/1‑93 (MQ=255)

                                       cTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATa                                                  >  2:1430329/1‑93 (MQ=255)

                                         tCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAAc                                                <  1:5224256/93‑1 (MQ=255)

                                          cgcgTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACt                                               <  1:1675494/93‑1 (MQ=255)

                                           gcgTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTc                                              <  1:6164649/93‑1 (MQ=255)

                                            cgTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCg                                             <  2:4408390/93‑1 (MQ=255)

                                             gTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGt                                            >  1:4193362/1‑93 (MQ=255)

                                              tGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGtt                                           <  1:2408614/93‑1 (MQ=255)

                                              tGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGtt                                           <  2:3596314/93‑1 (MQ=255)

                                               gAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTg                                          >  1:3857060/1‑93 (MQ=255)

                                                aaaTCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGc                                         <  2:5362400/93‑1 (MQ=255)

                                                aaaTCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGc                                         <  2:3522069/93‑1 (MQ=255)

                                                  aTCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTg                                       >  1:4691735/1‑93 (MQ=255)

                                                  aTCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTg                                       <  2:1076659/93‑1 (MQ=255)

                                                  aTCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTg                                       <  1:958712/93‑1 (MQ=255)

                                                   tCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTgg                                      <  2:6185509/93‑1 (MQ=255)

                                                     aTGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTgggg                                    >  1:4516758/1‑92 (MQ=255)

                                                     aTGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGc                                    <  2:3194899/93‑1 (MQ=255)

                                                     aTGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGc                                    <  2:371526/93‑1 (MQ=255)

                                                     aTGCAACGATATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGc                                    >  1:6314835/1‑93 (MQ=255)

                                                      tGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCg                                   <  2:682457/93‑1 (MQ=255)

                                                        caccGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGga                                 <  2:4501495/90‑1 (MQ=255)

                                                         aaCGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGgag                                >  1:2977958/1‑93 (MQ=255)

                                                           cGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGAc                              <  1:895552/93‑1 (MQ=255)

                                                           cGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGAc                              <  2:2202399/93‑1 (MQ=255)

                                                           cGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCAACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGAc                              <  2:5710900/93‑1 (MQ=255)

                                                            gTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACt                             >  1:3984660/1‑93 (MQ=255)

                                                             ttATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACtt                            >  1:5679109/1‑93 (MQ=255)

                                                              tATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTa                           <  2:2720714/93‑1 (MQ=255)

                                                              tATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTa                           >  2:6275784/1‑93 (MQ=255)

                                                                tCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGc                         <  1:1060626/93‑1 (MQ=255)

                                                                  cGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCaa                       >  2:4595121/1‑93 (MQ=255)

                                                                   gACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAAc                      >  2:3032780/1‑93 (MQ=255)

                                                                     cAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGc                    <  2:2314024/93‑1 (MQ=255)

                                                                       gTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCaa                  >  1:4419316/1‑93 (MQ=255)

                                                                          ccTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAatat               >  2:738795/1‑93 (MQ=255)

                                                                           cTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATc              >  1:1949244/1‑93 (MQ=255)

                                                                             aCTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTg            <  1:3749567/93‑1 (MQ=255)

                                                                                gtgATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATc         >  2:169057/1‑93 (MQ=255)

                                                                                gtgATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATc         <  2:1288058/93‑1 (MQ=255)

                                                                                 tgatgaTGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCg        <  1:4930603/93‑1 (MQ=255)

                                                                                 ggatgatCGGCTGGGCGCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTCCCTGGGCGGAGACTTAGCAACGCAATATCTGATCg        <  2:1536403/92‑1 (MQ=255)

                                                                                  gatgatGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGa       <  2:4077549/93‑1 (MQ=255)

                                                                                   atgatgGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATACAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGAt      <  1:2619345/93‑1 (MQ=255)

                                                                                    tgatgGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATa     <  2:6103254/93‑1 (MQ=255)

                                                                                      atgGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATaaa   >  1:1459271/1‑93 (MQ=255)

                                                                                      atgGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATaaa   >  1:5586703/1‑93 (MQ=255)

                                                                                       tgggctgggcTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAg  <  1:458473/93‑1 (MQ=255)

TTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAG  >  NC_000913/3936199‑3936378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCA  >  NC_000913/3936196‑3936382

GCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGG                                                                                               <  SRR4302534.1029442/94‑1 (MQ=60)

 CGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGC                                                                                              <  SRR4302534.5488515/94‑1 (MQ=60)

 CGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGC                                                                                              <  SRR4302534.3382766/94‑1 (MQ=60)

  GTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCT                                                                                             <  SRR4302534.4325699/94‑1 (MQ=60)

  GTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCT                                                                                             >  SRR4302534.72476/1‑94 (MQ=60)

  GTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGAGATGATGGGCT                                                                                             >  SRR4302534.4574974/1‑94 (MQ=60)

   TTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTG                                                                                            <  SRR4302534.2830327/94‑1 (MQ=60)

   TTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTG                                                                                            <  SRR4302534.283010/94‑1 (MQ=60)

   TTGATGACTTGCTGTTACTGTGCACCTAAACGCATCATCCTTCGCGTGAAAGCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTG                                                                                            <  SRR4302534.1182005/94‑1 (MQ=60)

    TGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGG                                                                                           >  SRR4302534.1960400/1‑94 (MQ=60)

    TGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGG                                                                                           >  SRR4302534.5340562/1‑94 (MQ=60)

    TGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGG                                                                                           >  SRR4302534.231544/1‑94 (MQ=60)

    TGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGG                                                                                           <  SRR4302534.5319229/94‑1 (MQ=60)

     GATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGG                                                                                          >  SRR4302534.95190/1‑94 (MQ=60)

     GATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGG                                                                                          >  SRR4302534.1338530/1‑94 (MQ=60)

        GGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTC                                                                                       >  SRR4302534.3577885/1‑94 (MQ=60)

         GCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCC                                                                                      <  SRR4302534.3546916/94‑1 (MQ=60)

            TGCTGTTACTGTGCGCCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTT                                                                                   <  SRR4302534.2822446/94‑1 (MQ=60)

            TGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTT                                                                                   <  SRR4302534.4114934/94‑1 (MQ=60)

            TGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTT                                                                                   <  SRR4302534.6016529/94‑1 (MQ=60)

             GCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTC                                                                                  <  SRR4302534.4047051/94‑1 (MQ=60)

             GCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTC                                                                                  <  SRR4302534.674961/94‑1 (MQ=60)

              CTCTTGCTGTGCACCGAAACGCATCAACTTTCGCGTGAAATCATGAAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCG                                                                                 <  SRR4302534.6435/94‑1 (MQ=60)

               TGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGA                                                                                >  SRR4302534.870503/1‑94 (MQ=60)

                GTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGAT                                                                               <  SRR4302534.6161947/94‑1 (MQ=60)

                GTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGAT                                                                               >  SRR4302534.6184163/1‑94 (MQ=60)

                 TTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTACGTTCGATG                                                                              <  SRR4302534.6477195/94‑1 (MQ=60)

                  TACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGG                                                                             >  SRR4302534.2766408/1‑94 (MQ=60)

                  TACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGG                                                                             >  SRR4302534.221785/1‑94 (MQ=60)

                  TACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGG                                                                             <  SRR4302534.3003269/94‑1 (MQ=60)

                  TACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGG                                                                             <  SRR4302534.2077158/94‑1 (MQ=60)

                  TACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGG                                                                             >  SRR4302534.533039/1‑94 (MQ=60)

                    CGGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCG                                                                           <  SRR4302534.5564526/94‑1 (MQ=60)

                      GTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGAC                                                                         <  SRR4302534.1785815/94‑1 (MQ=60)

                        GCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAG                                                                       >  SRR4302534.1814439/1‑94 (MQ=60)

                         CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGG                                                                      >  SRR4302534.1861705/1‑94 (MQ=60)

                          ACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCG                                                                     >  SRR4302534.4663766/1‑94 (MQ=60)

                            CGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGAT                                                                   >  SRR4302534.3112700/1‑94 (MQ=60)

                            CGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGAT                                                                   >  SRR4302534.5530226/1‑94 (MQ=60)

                            CGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGAT                                                                   <  SRR4302534.3685364/94‑1 (MQ=60)

                            CGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTGATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGAT                                                                   <  SRR4302534.383052/94‑1 (MQ=60)

                             GAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATC                                                                  <  SRR4302534.2158905/94‑1 (MQ=60)

                             GAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATC                                                                  <  SRR4302534.599859/94‑1 (MQ=60)

                              AAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCT                                                                 <  SRR4302534.2729338/94‑1 (MQ=60)

                              AAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCT                                                                 >  SRR4302534.1203468/1‑94 (MQ=60)

                              AAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCT                                                                 >  SRR4302534.5152760/1‑94 (MQ=60)

                              AAACGCATCAACCTTCGCGTCAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCT                                                                 >  SRR4302534.6456539/1‑94 (MQ=60)

                                ACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTA                                                               <  SRR4302534.4782922/94‑1 (MQ=60)

                                ACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTA                                                               <  SRR4302534.1821359/94‑1 (MQ=60)

                                  GCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATT                                                             >  SRR4302534.6451408/1‑94 (MQ=60)

                                  GCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATT                                                             <  SRR4302534.5357255/94‑1 (MQ=60)

                                   CATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTC                                                            <  SRR4302534.4772058/94‑1 (MQ=60)

                                   CATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTC                                                            <  SRR4302534.2195585/94‑1 (MQ=60)

                                   CATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGAGATCTTATTC                                                            >  SRR4302534.391806/1‑94 (MQ=60)

                                    ATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCA                                                           >  SRR4302534.1886554/1‑94 (MQ=60)

                                    ATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCA                                                           <  SRR4302534.1400354/94‑1 (MQ=60)

                                     TCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAG                                                          <  SRR4302534.3657261/94‑1 (MQ=60)

                                     TCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAG                                                          >  SRR4302534.5773721/1‑94 (MQ=60)

                                      CAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGG                                                         >  SRR4302534.3466918/1‑94 (MQ=60)

                                       AGCCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGA                                                        <  SRR4302534.889733/94‑1 (MQ=60)

                                       AACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGA                                                        >  SRR4302534.2260043/1‑94 (MQ=60)

                                        ACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGAT                                                       <  SRR4302534.2881948/94‑1 (MQ=60)

                                        ACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGAT                                                       >  SRR4302534.5982803/1‑94 (MQ=60)

                                        ACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGAT                                                       <  SRR4302534.720304/94‑1 (MQ=60)

                                         CCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATA                                                      >  SRR4302534.2506177/1‑94 (MQ=60)

                                            TCGCGTGAAATCATGCAGCGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACT                                                   <  SRR4302534.2282940/94‑1 (MQ=60)

                                            TCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACT                                                   <  SRR4302534.5365275/94‑1 (MQ=60)

                                             CGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTC                                                  <  SRR4302534.5457402/94‑1 (MQ=60)

                                             CGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTC                                                  >  SRR4302534.3091193/1‑94 (MQ=60)

                                             CGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTC                                                  <  SRR4302534.1719090/94‑1 (MQ=60)

                                             CGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTC                                                  <  SRR4302534.2087005/94‑1 (MQ=60)

                                              GCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCG                                                 <  SRR4302534.6332322/94‑1 (MQ=60)

                                              GCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCG                                                 <  SRR4302534.2587014/94‑1 (MQ=60)

                                              GCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCG                                                 <  SRR4302534.6332320/94‑1 (MQ=60)

                                               CGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGT                                                >  SRR4302534.4306646/1‑94 (MQ=60)

                                                GTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTT                                               <  SRR4302534.4467648/94‑1 (MQ=60)

                                                GTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTT                                               <  SRR4302534.2196276/94‑1 (MQ=60)

                                                GTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTT                                               <  SRR4302534.2821383/94‑1 (MQ=60)

                                                 TGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTG                                              <  SRR4302534.1342133/94‑1 (MQ=60)

                                                 TGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTG                                              >  SRR4302534.3960606/1‑94 (MQ=60)

                                                 TGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTG                                              <  SRR4302534.2472639/94‑1 (MQ=60)

                                                  GAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGC                                             >  SRR4302534.4788974/1‑94 (MQ=60)

                                                   AAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCT                                            <  SRR4302534.3291310/94‑1 (MQ=60)

                                                    AATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTG                                           >  SRR4302534.4819090/1‑94 (MQ=60)

                                                    AATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTG                                           <  SRR4302534.3872967/94‑1 (MQ=60)

                                                     ATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGG                                          <  SRR4302534.983138/94‑1 (MQ=60)

                                                     ATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGG                                          >  SRR4302534.1445396/1‑94 (MQ=60)

                                                     ATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGG                                          >  SRR4302534.5023230/1‑94 (MQ=60)

                                                     ATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGG                                          <  SRR4302534.4437610/94‑1 (MQ=60)

                                                       CATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGG                                        >  SRR4302534.4639101/1‑94 (MQ=60)

                                                       CATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGC                                        >  SRR4302534.4166211/1‑94 (MQ=60)

                                                       CATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGATGGGC                                        >  SRR4302534.1010320/1‑94 (MQ=60)

                                                       CATGCAACGATATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGC                                        >  SRR4302534.6485522/1‑94 (MQ=60)

                                                          GCAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGA                                     <  SRR4302534.45457/94‑1 (MQ=60)

                                                           CAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAG                                    >  SRR4302534.3055899/1‑94 (MQ=60)

                                                           CAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAG                                    <  SRR4302534.913357/94‑1 (MQ=60)

                                                           CAACGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAG                                    <  SRR4302534.4679099/94‑1 (MQ=60)

                                                              CGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACT                                 >  SRR4302534.4091287/1‑94 (MQ=60)

                                                              CGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACT                                 <  SRR4302534.918246/94‑1 (MQ=60)

                                                              CGTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACT                                 >  SRR4302534.159568/1‑94 (MQ=60)

                                                               GTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTT                                >  SRR4302534.5832867/1‑94 (MQ=60)

                                                               GTTATCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTT                                <  SRR4302534.94537/94‑1 (MQ=60)

                                                                   TCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCA                            <  SRR4302534.5874132/94‑1 (MQ=60)

                                                                   TCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCA                            <  SRR4302534.1088117/94‑1 (MQ=60)

                                                                   TCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCA                            <  SRR4302534.5606287/94‑1 (MQ=60)

                                                                   TCCGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCA                            >  SRR4302534.2876359/1‑94 (MQ=60)

                                                                     CGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAAC                          >  SRR4302534.2414172/1‑94 (MQ=60)

                                                                     CGACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAAC                          <  SRR4302534.2711259/94‑1 (MQ=60)

                                                                      cgcaaTGCCTACTGTGAGGATGGGCTGGGGCTCGTTCCATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACG                         <  SRR4302534.3100050/89‑1 (MQ=60)

                                                                      GACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACG                         <  SRR4302534.400923/94‑1 (MQ=60)

                                                                      GACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACG                         <  SRR4302534.6627744/94‑1 (MQ=60)

                                                                      GACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACG                         <  SRR4302534.1454278/94‑1 (MQ=60)

                                                                      GACAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACG                         <  SRR4302534.5469937/94‑1 (MQ=60)

                                                                      GACAGTGCCTACTGTGATGATGGGCTGGCCTCAGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACG                         <  SRR4302534.3736824/94‑1 (MQ=60)

                                                                        CAGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCA                       <  SRR4302534.1997179/94‑1 (MQ=60)

                                                                         AGTGCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAA                      >  SRR4302534.4538631/1‑94 (MQ=60)

                                                                            GCCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATAT                   <  SRR4302534.5863410/94‑1 (MQ=60)

                                                                             CCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATC                  >  SRR4302534.5811178/1‑94 (MQ=60)

                                                                             CCTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATC                  >  SRR4302534.2000261/1‑94 (MQ=60)

                                                                              CTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCT                 >  SRR4302534.1679744/1‑94 (MQ=60)

                                                                              CTACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCT                 >  SRR4302534.3109378/1‑94 (MQ=60)

                                                                                ACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGA               <  SRR4302534.3850279/94‑1 (MQ=60)

                                                                                ACTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGA               <  SRR4302534.598025/94‑1 (MQ=60)

                                                                                 CTGTGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGAT              <  SRR4302534.6428004/94‑1 (MQ=60)

                                                                                    TGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGA           <  SRR4302534.5064238/94‑1 (MQ=60)

                                                                                    TGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGA           >  SRR4302534.2468544/1‑94 (MQ=60)

                                                                                    TGATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTCGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGA           <  SRR4302534.1976288/94‑1 (MQ=60)

                                                                                     GATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGAT          <  SRR4302534.4714352/94‑1 (MQ=60)

                                                                                      ATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATA         >  SRR4302534.2894446/1‑94 (MQ=60)

                                                                                      ATGATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATACAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATA         <  SRR4302534.2688656/94‑1 (MQ=60)

                                                                                        GATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAA       <  SRR4302534.848362/94‑1 (MQ=60)

                                                                                        GATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAA       >  SRR4302534.1497653/1‑94 (MQ=60)

                                                                                        GATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAA       >  SRR4302534.5737692/1‑94 (MQ=60)

                                                                                        GATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAA       >  SRR4302534.1157841/1‑94 (MQ=60)

                                                                                        GATGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAA       <  SRR4302534.4731933/94‑1 (MQ=60)

                                                                                          TGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGG     <  SRR4302534.116126/94‑1 (MQ=60)

                                                                                          TGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGG     <  SRR4302534.470060/94‑1 (MQ=60)

                                                                                          TGGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGG     <  SRR4302534.4899992/94‑1 (MQ=60)

                                                                                           GGGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGA    >  SRR4302534.5174279/1‑94 (MQ=60)

                                                                                            GGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTC   >  SRR4302534.102507/1‑94 (MQ=60)

                                                                                            GGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTC   <  SRR4302534.1446114/94‑1 (MQ=60)

                                                                                            GGCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTC   >  SRR4302534.657695/1‑94 (MQ=60)

                                                                                             GCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCG  <  SRR4302534.3072675/94‑1 (MQ=60)

                                                                                             GCTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCA  <  SRR4302534.834055/94‑1 (MQ=60)

GCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCA  >  NC_000913/3936196‑3936382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`