Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,937,900 G→A G113G (GGC→GGT)  yieO ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,937,9000GA99.6% 899.7 / ‑5.2 239G113G (GGC→GGT) yieOputative transport protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (129/109);  total (130/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 253 total aligned reads displayed.

CGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACT  >  NC_000913/3937810‑3937989
                                                                                          |                                                                                         
cgacATGGCGACAAAATTCAATACTGGAAGAAGTTCATGACGAGGATAAGCGCTCATTAAGGCCAGCCGAGCAACAGGCATCATCATTGcacc                                                                                         <  1:5351332/90‑1 (MQ=255)
cGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGcacc                                                                                         >  1:1181342/1‑93 (MQ=255)
cGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGcacc                                                                                         >  2:1283616/1‑93 (MQ=255)
  gCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGcaccgc                                                                                       <  2:3930925/93‑1 (MQ=255)
    aTGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCt                                                                                     <  2:5076053/93‑1 (MQ=255)
    aTGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCt                                                                                     >  2:3823528/1‑93 (MQ=255)
    aTGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCt                                                                                     <  2:6272350/93‑1 (MQ=255)
      ggCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTAt                                                                                   >  2:4690427/1‑93 (MQ=255)
      ggCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTAt                                                                                   >  2:5617063/1‑93 (MQ=255)
       gCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATc                                                                                  >  2:2074022/1‑93 (MQ=255)
        cGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATcc                                                                                 <  1:1623243/93‑1 (MQ=255)
        cGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATcc                                                                                 >  2:2764021/1‑93 (MQ=255)
         gACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATccc                                                                                >  1:660149/1‑93 (MQ=255)
         gACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATccc                                                                                >  1:5900302/1‑93 (MQ=255)
          aCAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATcccc                                                                               <  2:2166362/93‑1 (MQ=255)
           cAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCt                                                                              <  2:6198993/93‑1 (MQ=255)
            aaaaTTCAATACTGGAAGAAGTTCATTACGAGGAGAAGCGCGCAGTAAGGCCAGCGGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTg                                                                             <  2:2490310/93‑1 (MQ=255)
             aaaTTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGa                                                                            >  2:5002202/1‑93 (MQ=255)
              aaTTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGaa                                                                           >  1:2534218/1‑93 (MQ=255)
               aTTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAAt                                                                          <  1:6096366/93‑1 (MQ=255)
               aTTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAAt                                                                          <  2:2912564/93‑1 (MQ=255)
                ttCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATa                                                                         >  2:274723/1‑93 (MQ=255)
                 tCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATaa                                                                        >  1:1341527/1‑93 (MQ=255)
                  cAATACTGGAAGCAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAAc                                                                       >  1:6022861/1‑93 (MQ=255)
                  cAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAAc                                                                       >  2:6436136/1‑93 (MQ=255)
                  cAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAAc                                                                       >  1:2667561/1‑93 (MQ=255)
                    aTACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAAccc                                                                     >  2:1396738/1‑93 (MQ=255)
                     tACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCg                                                                    <  1:5523317/93‑1 (MQ=255)
                      aCTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCgg                                                                   >  2:4799422/1‑93 (MQ=255)
                      aCTGGAAGAAGTTCATTACGAGGATAAGCGCGAAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCgg                                                                   <  1:6175108/93‑1 (MQ=255)
                       cTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGa                                                                  <  2:3258957/93‑1 (MQ=255)
                        tGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGaa                                                                 <  2:6143886/93‑1 (MQ=255)
                          gaagaaGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGa                                                               >  1:2713041/1‑93 (MQ=255)
                          gaagaaGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGa                                                               >  1:728713/1‑93 (MQ=255)
                          gaagaaGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGa                                                               <  1:3913654/93‑1 (MQ=255)
                             gaagTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACaa                                                            >  1:4048700/1‑93 (MQ=255)
                               atttCATTACGAGGATAAGCTCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAAcc                                                          <  1:1456762/91‑1 (MQ=255)
                               agTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAAcc                                                          <  2:1197593/93‑1 (MQ=255)
                               agTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAAcc                                                          >  2:3168700/1‑93 (MQ=255)
                                gTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCa                                                         <  2:3299826/93‑1 (MQ=255)
                                gTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCa                                                         <  2:3463194/93‑1 (MQ=255)
                                 ttCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAg                                                        >  1:805420/1‑93 (MQ=255)
                                  tCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGc                                                       <  2:4038712/93‑1 (MQ=255)
                                   cATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATGCTCATTGCACCGGCTATCCCCTGAATAACCCGCAAGACAACCAGCt                                                      >  1:4371259/1‑93 (MQ=255)
                                   cATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCt                                                      >  1:3045426/1‑93 (MQ=255)
                                   cATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCt                                                      >  1:4858042/1‑93 (MQ=255)
                                   cATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCt                                                      >  2:5560923/1‑93 (MQ=255)
                                    aTTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCtg                                                     >  2:5332576/1‑93 (MQ=255)
                                     ttACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCtgt                                                    <  2:734240/93‑1 (MQ=255)
                                     ttACGAGGAGAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCtgt                                                    <  2:3600571/93‑1 (MQ=255)
                                      tACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCtgtg                                                   <  1:1872373/93‑1 (MQ=255)
                                         gAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTa                                                <  2:451113/93‑1 (MQ=255)
                                         gAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTa                                                >  1:1935675/1‑93 (MQ=255)
                                           ggATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGc                                              >  1:6130165/1‑93 (MQ=255)
                                           ggATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGc                                              >  1:1114578/1‑93 (MQ=255)
                                           ggATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGc                                              <  2:433408/93‑1 (MQ=255)
                                           ggATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGc                                              <  1:5165620/93‑1 (MQ=255)
                                            gATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCg                                             >  2:4103486/1‑93 (MQ=255)
                                            gATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCg                                             <  1:2230645/93‑1 (MQ=255)
                                            gATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCg                                             >  2:5900483/1‑93 (MQ=255)
                                            gATAAGCGCGCAGCAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCg                                             >  1:1652898/1‑93 (MQ=255)
                                             aTAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGa                                            >  2:4490186/1‑93 (MQ=255)
                                             aTAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGa                                            <  2:2381446/93‑1 (MQ=255)
                                              tAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGaa                                           >  1:1376258/1‑93 (MQ=255)
                                              tAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGaa                                           >  1:6440893/1‑93 (MQ=255)
                                               aaGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTTTCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAAt                                          <  1:3556608/93‑1 (MQ=255)
                                               aaGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAAt                                          <  1:1770218/93‑1 (MQ=255)
                                               aaGCGCGCAGTAAGCCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAAt                                          <  2:1777027/93‑1 (MQ=255)
                                                aGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAAtt                                         >  1:1433946/1‑93 (MQ=255)
                                                 gcgcgcAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTa                                        <  2:5791378/93‑1 (MQ=255)
                                                   gcgcAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGa                                      >  2:1480874/1‑93 (MQ=255)
                                                     gcAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGaaa                                    <  2:2539889/93‑1 (MQ=255)
                                                     gcAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGaaa                                    <  1:931290/93‑1 (MQ=255)
                                                      cAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAg                                   <  1:4540173/93‑1 (MQ=255)
                                                      cAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAg                                   >  1:504768/1‑93 (MQ=255)
                                                        gTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTg                                 >  1:338672/1‑93 (MQ=255)
                                                          aaGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTgcg                               <  1:5663197/93‑1 (MQ=255)
                                                           aGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTgcgc                              <  1:4195324/93‑1 (MQ=255)
                                                           aGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTgcgc                              <  2:5817254/93‑1 (MQ=255)
                                                           aGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTgcgc                              >  2:5548762/1‑93 (MQ=255)
                                                             gCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAg                            >  2:370743/1‑93 (MQ=255)
                                                             gCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAg                            <  1:1224047/93‑1 (MQ=255)
                                                               cAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGc                          <  1:1025625/93‑1 (MQ=255)
                                                                aGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGca                         >  2:5416024/1‑92 (MQ=255)
                                                                 gCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCa                        <  2:5186674/93‑1 (MQ=255)
                                                                 gCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCa                        >  1:6266329/1‑93 (MQ=255)
                                                                   cGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCaga                      >  2:5580990/1‑93 (MQ=255)
                                                                   cGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCaga                      <  1:2401080/93‑1 (MQ=255)
                                                                    gAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCagag                     >  2:5664363/1‑93 (MQ=255)
                                                                    gAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCagag                     >  1:2902472/1‑93 (MQ=255)
                                                                    gAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCagag                     >  2:3013729/1‑93 (MQ=255)
                                                                       cAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAAc                  <  1:4408870/93‑1 (MQ=255)
                                                                       cAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAAc                  >  2:1862883/1‑93 (MQ=255)
                                                                            ggCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAAt             >  2:892613/1‑93 (MQ=255)
                                                                             gCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAAtg            <  2:1671389/93‑1 (MQ=255)
                                                                                tcatcatTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGa         <  1:4257487/93‑1 (MQ=255)
                                                                                   tcatTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACa      >  2:5198917/1‑93 (MQ=255)
                                                                                   tcatTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACa      <  2:3872217/93‑1 (MQ=255)
                                                                                      ttGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGAc   >  1:3216531/1‑93 (MQ=255)
                                                                                       tGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACt  <  2:4284698/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACT  >  NC_000913/3937810‑3937989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCAC  >  NC_000913/3937807‑3937992
                                                                                             |                                                                                            
ACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCA                                                                                              >  SRR4302534.5422155/1‑94 (MQ=60)
ACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCA                                                                                              <  SRR4302534.2328077/94‑1 (MQ=60)
ACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCA                                                                                              >  SRR4302534.1174428/1‑94 (MQ=60)
ACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCA                                                                                              >  SRR4302534.403824/1‑94 (MQ=60)
 CCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCAC                                                                                             >  SRR4302534.1492548/1‑94 (MQ=60)
  CCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACC                                                                                            >  SRR4302534.1212395/1‑94 (MQ=60)
  CCGGCATGGCGACAAAATTCAATACTCGAAGAAGTTCATTACGAGGGTAAGCGCGCAGTAAGGCCAGCCGACCAACAGGCATCATCCTTGCACC                                                                                            >  SRR4302534.3762998/1‑94 (MQ=60)
   CGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCG                                                                                           >  SRR4302534.3332813/1‑94 (MQ=60)
   CGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCG                                                                                           <  SRR4302534.6570251/94‑1 (MQ=60)
   CGACATGGCGACAAAATTCAATACTGGAAGAAGTTCATGACGAGGATAAGCGCTCATTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCG                                                                                           <  SRR4302534.5495610/94‑1 (MQ=60)
       ATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTA                                                                                       >  SRR4302534.1434662/1‑94 (MQ=60)
        TGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTAT                                                                                      <  SRR4302534.750059/94‑1 (MQ=60)
         GGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATC                                                                                     >  SRR4302534.916125/1‑94 (MQ=60)
          GCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCC                                                                                    <  SRR4302534.6277632/94‑1 (MQ=60)
          GCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCC                                                                                    >  SRR4302534.6107145/1‑94 (MQ=60)
          GCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCC                                                                                    <  SRR4302534.5078064/94‑1 (MQ=60)
           CGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCC                                                                                   >  SRR4302534.5679792/1‑94 (MQ=60)
           CGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCC                                                                                   <  SRR4302534.2492753/94‑1 (MQ=60)
           CGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCC                                                                                   <  SRR4302534.1665591/94‑1 (MQ=60)
           CGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCC                                                                                   >  SRR4302534.6060504/1‑94 (MQ=60)
           CGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCC                                                                                   >  SRR4302534.676199/1‑94 (MQ=60)
            GACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCC                                                                                  <  SRR4302534.1949045/94‑1 (MQ=60)
                AAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAA                                                                              >  SRR4302534.2601449/1‑94 (MQ=60)
                 AATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAAT                                                                             >  SRR4302534.6317020/1‑94 (MQ=60)
                  ATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATA                                                                            <  SRR4302534.6262154/94‑1 (MQ=60)
                   TTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAA                                                                           >  SRR4302534.1376893/1‑94 (MQ=60)
                   TTCAATACTGGAAGAAGTTCATTACGAGGATAAGCACGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAA                                                                           <  SRR4302534.1852995/94‑1 (MQ=60)
                    TCAATACTGGAAGCAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAAC                                                                          >  SRR4302534.6186550/1‑94 (MQ=60)
                    TCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAAC                                                                          <  SRR4302534.4393029/94‑1 (MQ=60)
                    TCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAAC                                                                          >  SRR4302534.2738067/1‑94 (MQ=60)
                     CAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACC                                                                         <  SRR4302534.1085993/94‑1 (MQ=60)
                      AATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCC                                                                        >  SRR4302534.6403134/1‑94 (MQ=60)
                       ATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCG                                                                       <  SRR4302534.6248054/94‑1 (MQ=60)
                        atcgaGAAGAAGTGCATCACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGG                                                                      <  SRR4302534.2818281/89‑1 (MQ=60)
                        TACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGG                                                                      <  SRR4302534.5856197/94‑1 (MQ=60)
                        TACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGG                                                                      <  SRR4302534.5672372/94‑1 (MQ=60)
                         ACTGGAAGAAGTTCATTACGAGGATAAGCGCGAAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGA                                                                     <  SRR4302534.6343135/94‑1 (MQ=60)
                           TGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAG                                                                   >  SRR4302534.1456150/1‑94 (MQ=60)
                            GGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGA                                                                  >  SRR4302534.528074/1‑94 (MQ=60)
                            GGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGA                                                                  >  SRR4302534.746709/1‑94 (MQ=60)
                            GGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGA                                                                  <  SRR4302534.3689608/94‑1 (MQ=60)
                            GGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGA                                                                  >  SRR4302534.2784669/1‑94 (MQ=60)
                             GAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGAC                                                                 >  SRR4302534.411362/1‑94 (MQ=60)
                             GAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGAC                                                                 <  SRR4302534.4018586/94‑1 (MQ=60)
                               AGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAA                                                               >  SRR4302534.4157281/1‑94 (MQ=60)
                                GAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAAC                                                              >  SRR4302534.1242547/1‑94 (MQ=60)
                                 AAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACC                                                             >  SRR4302534.2665008/1‑94 (MQ=60)
                                 AAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACC                                                             >  SRR4302534.172895/1‑94 (MQ=60)
                                  ATTTCATTACGAGGATAAGCTCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCA                                                            <  SRR4302534.1495081/94‑1 (MQ=60)
                                  AGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCA                                                            >  SRR4302534.5112864/1‑94 (MQ=60)
                                   GTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAG                                                           >  SRR4302534.825655/1‑94 (MQ=60)
                                     TCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATGCTCATTGCACCGGCTATCCCCTGAATAACCCGCAAGACAACCAGCT                                                         >  SRR4302534.4489032/1‑94 (MQ=60)
                                     TCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCT                                                         >  SRR4302534.4989844/1‑94 (MQ=60)
                                     TCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCT                                                         >  SRR4302534.3125305/1‑94 (MQ=60)
                                      CATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTG                                                        >  SRR4302534.1686815/1‑94 (MQ=60)
                                         TACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGG                                                     <  SRR4302534.1921099/94‑1 (MQ=60)
                                          ACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGT                                                    <  SRR4302534.22979/94‑1 (MQ=60)
                                           CGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTA                                                   >  SRR4302534.1986287/1‑94 (MQ=60)
                                           CGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTA                                                   <  SRR4302534.5343502/94‑1 (MQ=60)
                                           CGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTA                                                   >  SRR4302534.6651905/1‑94 (MQ=60)
                                            GAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGCAGACAACCAGCTGTGGTAG                                                  >  SRR4302534.72929/1‑94 (MQ=60)
                                            GAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAG                                                  >  SRR4302534.229164/1‑94 (MQ=60)
                                            GAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAG                                                  >  SRR4302534.4292798/1‑94 (MQ=60)
                                             AGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGC                                                 >  SRR4302534.6296910/1‑94 (MQ=60)
                                             AGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGC                                                 <  SRR4302534.6475121/94‑1 (MQ=60)
                                             AGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGC                                                 >  SRR4302534.5559425/1‑94 (MQ=60)
                                             AGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGC                                                 >  SRR4302534.1143676/1‑94 (MQ=60)
                                              GGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCG                                                >  SRR4302534.1181168/1‑94 (MQ=60)
                                              GGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCG                                                <  SRR4302534.5304863/94‑1 (MQ=60)
                                              GGATAAGCGCGCAGCAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCG                                                >  SRR4302534.1695973/1‑94 (MQ=60)
                                               GATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGA                                               <  SRR4302534.2289722/94‑1 (MQ=60)
                                                ATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAA                                              >  SRR4302534.1412514/1‑94 (MQ=60)
                                                ATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAA                                              >  SRR4302534.6614471/1‑94 (MQ=60)
                                                  AAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTTTCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATT                                            <  SRR4302534.3652304/94‑1 (MQ=60)
                                                  AAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATT                                            <  SRR4302534.1816180/94‑1 (MQ=60)
                                                  AAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATT                                            >  SRR4302534.1471678/1‑94 (MQ=60)
                                                  AAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATT                                            >  SRR4302534.311418/1‑94 (MQ=60)
                                                   AGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTA                                           <  SRR4302534.2612063/94‑1 (MQ=60)
                                                     CGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGA                                         >  SRR4302534.5992003/1‑94 (MQ=60)
                                                      GCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAA                                        <  SRR4302534.543756/94‑1 (MQ=60)
                                                        GCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAG                                      >  SRR4302534.517322/1‑94 (MQ=60)
                                                        GCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAG                                      <  SRR4302534.954978/94‑1 (MQ=60)
                                                        GCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAG                                      >  SRR4302534.3202160/1‑94 (MQ=60)
                                                         CAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGT                                     <  SRR4302534.4719624/94‑1 (MQ=60)
                                                         CAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGT                                     <  SRR4302534.4663247/94‑1 (MQ=60)
                                                          AGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTG                                    >  SRR4302534.347338/1‑94 (MQ=60)
                                                           GTAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGC                                   >  SRR4302534.3463234/1‑94 (MQ=60)
                                                            TAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCG                                  >  SRR4302534.2744667/1‑94 (MQ=60)
                                                            TAAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCG                                  <  SRR4302534.2793588/94‑1 (MQ=60)
                                                             AAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGC                                 >  SRR4302534.196604/1‑94 (MQ=60)
                                                             AAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGC                                 <  SRR4302534.3572031/94‑1 (MQ=60)
                                                             AAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGC                                 <  SRR4302534.5816507/94‑1 (MQ=60)
                                                             AAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGC                                 >  SRR4302534.1512268/1‑94 (MQ=60)
                                                             AAGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGC                                 >  SRR4302534.4466679/1‑94 (MQ=60)
                                                              AGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCA                                <  SRR4302534.4308656/94‑1 (MQ=60)
                                                              AGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCA                                >  SRR4302534.4493426/1‑94 (MQ=60)
                                                              AGGCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCA                                <  SRR4302534.1376090/94‑1 (MQ=60)
                                                                GCCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGG                              <  SRR4302534.1256280/94‑1 (MQ=60)
                                                                 CCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGC                             >  SRR4302534.297765/1‑94 (MQ=60)
                                                                 CCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGC                             >  SRR4302534.4574322/1‑94 (MQ=60)
                                                                 CCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGC                             >  SRR4302534.1383915/1‑94 (MQ=60)
                                                                 CCAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGC                             >  SRR4302534.917995/1‑94 (MQ=60)
                                                                  CAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCC                            <  SRR4302534.1052046/94‑1 (MQ=60)
                                                                  CAGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCC                            >  SRR4302534.1165482/1‑94 (MQ=60)
                                                                   AGCCGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCA                           >  SRR4302534.6436031/1‑94 (MQ=60)
                                                                      CGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAG                        >  SRR4302534.2978638/1‑94 (MQ=60)
                                                                      CGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAG                        >  SRR4302534.4343486/1‑94 (MQ=60)
                                                                      CGAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAG                        <  SRR4302534.2464901/94‑1 (MQ=60)
                                                                       GAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGA                       >  SRR4302534.2773594/1‑94 (MQ=60)
                                                                       GAGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGA                       <  SRR4302534.4304758/94‑1 (MQ=60)
                                                                        AGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAA                      >  SRR4302534.1730120/1‑94 (MQ=60)
                                                                        AGCAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAA                      >  SRR4302534.165849/1‑94 (MQ=60)
                                                                          CAACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACC                    <  SRR4302534.4527861/94‑1 (MQ=60)
                                                                           AACAGGCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCC                   >  SRR4302534.703777/1‑94 (MQ=60)
                                                                                GCATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGT              >  SRR4302534.6318164/1‑94 (MQ=60)
                                                                                 CATCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTG             <  SRR4302534.2128305/94‑1 (MQ=60)
                                                                                   TCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAA           <  SRR4302534.4579952/94‑1 (MQ=60)
                                                                                   TCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAA           <  SRR4302534.5547336/94‑1 (MQ=60)
                                                                                   TCATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAA           <  SRR4302534.4372420/94‑1 (MQ=60)
                                                                                     ATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACA         >  SRR4302534.6253121/1‑94 (MQ=60)
                                                                                     ATCATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACA         <  SRR4302534.1473775/94‑1 (MQ=60)
                                                                                        ATTGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGAC      >  SRR4302534.3301904/1‑94 (MQ=60)
                                                                                          TGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGTACAGACTC    >  SRR4302534.6065710/1‑94 (MQ=60)
                                                                                          TGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTC    >  SRR4302534.4682505/1‑94 (MQ=60)
                                                                                          TGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTC    >  SRR4302534.3075688/1‑94 (MQ=60)
                                                                                          TGCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTC    <  SRR4302534.1261775/94‑1 (MQ=60)
                                                                                           GCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCA   >  SRR4302534.5890678/1‑94 (MQ=60)
                                                                                           GCACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCA   <  SRR4302534.5431311/94‑1 (MQ=60)
                                                                                            CACCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCAC  >  SRR4302534.4856350/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAACCAGCTGTGGTAGCGAATTAGAAAGTGCGCAGGCCAGAGAACCCAATGTGAACAGACTCAC  >  NC_000913/3937807‑3937992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑