Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,964,491	A→G	T154A (ACT→GCT)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,964,491	0	A	G	99.6%	828.9 / NA	235	T154A (ACT→GCT)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (137/97); total (138/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

CCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGT  >  NC_000913/3964401‑3964580

ccTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCACGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTCGTAACGGTTCTGCt                                                                                         >  1:3383605/1‑93 (MQ=255)

 cTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTctgctg                                                                                        >  2:4329062/1‑93 (MQ=255)

   gAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGaa                                                                                      <  1:4347051/93‑1 (MQ=255)

      aaCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAt                                                                                   >  2:2958183/1‑93 (MQ=255)

      aaCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAt                                                                                   <  1:1570033/93‑1 (MQ=255)

      aaCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAt                                                                                   <  2:3960547/93‑1 (MQ=255)

       aCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAtt                                                                                  <  1:986038/93‑1 (MQ=255)

        cGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAttt                                                                                 <  1:2018600/93‑1 (MQ=255)

          cccGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTaa                                                                               >  1:4592829/1‑93 (MQ=255)

            cGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACt                                                                             <  1:3086292/93‑1 (MQ=255)

             gCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTg                                                                            >  2:4716887/1‑93 (MQ=255)

             gCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTg                                                                            >  2:4768642/1‑93 (MQ=255)

             gCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTg                                                                            >  1:6110511/1‑93 (MQ=255)

             gCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTg                                                                            >  1:5433365/1‑93 (MQ=255)

              caacaaAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGc                                                                           >  2:5742963/1‑93 (MQ=255)

               aacaaAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCt                                                                          >  1:803550/1‑93 (MQ=255)

               aacaaAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCt                                                                          >  1:3254663/1‑93 (MQ=255)

               aacaaAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCt                                                                          >  2:928745/1‑93 (MQ=255)

                acaaAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTc                                                                         >  1:5925018/1‑93 (MQ=255)

                  aaaaTCCTCTTTGAGAACTTATCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTcgc                                                                       <  2:5912290/93‑1 (MQ=255)

                  aaaaTCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTcgc                                                                       >  2:2349502/1‑93 (MQ=255)

                   aaaTCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTcgcg                                                                      >  2:3045896/1‑93 (MQ=255)

                    aaTCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGt                                                                     <  2:5104484/93‑1 (MQ=255)

                    aaTCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGt                                                                     <  1:1226576/93‑1 (MQ=255)

                       ccTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACt                                                                  <  2:1140472/93‑1 (MQ=255)

                         tctTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTgg                                                                >  2:2193175/1‑93 (MQ=255)

                          ctTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGa                                                               >  2:95445/1‑93 (MQ=255)

                           tttGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGAt                                                              >  2:1482656/1‑93 (MQ=255)

                           tttGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGAt                                                              <  2:5607195/93‑1 (MQ=255)

                           tttGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGAt                                                              <  1:1442317/93‑1 (MQ=255)

                           tatGAGAACTTATCCCCGCTGCACGCAACCTCTCGTCTGCGAATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGAt                                                              <  1:163442/91‑1 (MQ=255)

                             tGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCt                                                            <  1:2575646/93‑1 (MQ=255)

                             tGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCt                                                            <  2:668918/93‑1 (MQ=255)

                              gagaACTTACCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTg                                                           <  1:4430682/93‑1 (MQ=255)

                              gagaACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTg                                                           <  1:2765970/93‑1 (MQ=255)

                              gagaACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTg                                                           >  2:2813726/1‑93 (MQ=255)

                               agaACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTgg                                                          >  1:4884089/1‑93 (MQ=255)

                                gaACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGc                                                         >  2:5278835/1‑93 (MQ=255)

                                gaACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGc                                                         <  2:3205371/93‑1 (MQ=255)

                                gaACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGc                                                         >  2:788148/1‑93 (MQ=255)

                                  aCTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCAt                                                       >  1:537380/1‑93 (MQ=255)

                                   cTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATc                                                      >  1:451617/1‑93 (MQ=255)

                                      aaCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCAcc                                                   <  2:4682244/93‑1 (MQ=255)

                                       aCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCt                                                  >  1:368188/1‑93 (MQ=255)

                                        ccccGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTa                                                 <  2:5446523/93‑1 (MQ=255)

                                        ccccGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTa                                                 <  2:2510305/93‑1 (MQ=255)

                                        ccccGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTa                                                 >  2:389281/1‑93 (MQ=255)

                                        ccccGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAATTAACTGCTCGCGTACTGGATCTGGCATCACCTa                                                 >  1:4957302/1‑93 (MQ=255)

                                          ccGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATc                                               >  2:1579901/1‑93 (MQ=255)

                                          ccGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTAGGATCACCTATc                                               >  1:677398/1‑93 (MQ=255)

                                            gCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCgg                                             >  1:2671329/1‑93 (MQ=255)

                                             cTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGt                                            <  2:2710832/93‑1 (MQ=255)

                                              tGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTc                                           >  1:391556/1‑93 (MQ=255)

                                              tGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTc                                           >  2:4029360/1‑93 (MQ=255)

                                               gCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCg                                          >  2:4410374/1‑93 (MQ=255)

                                                 aCGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTg                                        >  1:1025558/1‑93 (MQ=255)

                                                 aCGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTg                                        >  2:3062969/1‑93 (MQ=255)

                                                  cGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTgg                                       >  1:4385481/1‑93 (MQ=255)

                                                   gCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGt                                      >  2:2394832/1‑93 (MQ=255)

                                                    cAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTc                                     >  2:150456/1‑93 (MQ=255)

                                                     aaaCTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCa                                    >  1:3620937/1‑93 (MQ=255)

                                                     aaaCTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCa                                    >  1:5114179/1‑93 (MQ=255)

                                                       aCTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGc                                  >  2:1111922/1‑93 (MQ=255)

                                                       aCTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGc                                  >  2:806470/1‑93 (MQ=255)

                                                        ctctCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCg                                 >  2:6322417/1‑93 (MQ=255)

                                                          ctcGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTg                               >  2:4152043/1‑93 (MQ=255)

                                                          ctcGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTg                               >  2:178274/1‑93 (MQ=255)

                                                          ctcGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTg                               <  1:1773709/93‑1 (MQ=255)

                                                           tcGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTgg                              <  1:4768642/93‑1 (MQ=255)

                                                            cGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGt                             <  2:6019351/93‑1 (MQ=255)

                                                             gTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTc                            >  1:1155216/1‑93 (MQ=255)

                                                             gTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTc                            >  2:2589841/1‑93 (MQ=255)

                                                              tCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCt                           >  1:1501160/1‑93 (MQ=255)

                                                              tCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCt                           >  2:1880575/1‑93 (MQ=255)

                                                               cTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTg                          <  2:4394974/93‑1 (MQ=255)

                                                                tGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGa                         <  2:6388313/93‑1 (MQ=255)

                                                                tGCGTATGGAACGTGGTAAAGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGa                         >  1:6279928/1‑93 (MQ=255)

                                                                 gCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGAt                        >  1:4134760/1‑93 (MQ=255)

                                                                    tATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATtgt                     <  1:5344492/93‑1 (MQ=255)

                                                                    tATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATtgt                     <  1:973307/93‑1 (MQ=255)

                                                                      tGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTgg                   <  1:5812838/93‑1 (MQ=255)

                                                                       ggAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGc                  <  1:2136467/93‑1 (MQ=255)

                                                                       ggAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGc                  <  2:1053011/93‑1 (MQ=255)

                                                                        gAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCa                 >  1:547255/1‑93 (MQ=255)

                                                                        gAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCa                 <  2:2860121/93‑1 (MQ=255)

                                                                        gAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCa                 >  1:729446/1‑93 (MQ=255)

                                                                          gcGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCAcc               >  1:3566633/2‑93 (MQ=255)

                                                                          aCGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCAcc               >  2:3843134/1‑93 (MQ=255)

                                                                           cGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCAccg              >  1:5961312/1‑93 (MQ=255)

                                                                             tGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCAccgcc            >  2:6427040/1‑93 (MQ=255)

                                                                              ggTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCAccgccg           >  2:3377396/1‑93 (MQ=255)

                                                                              ggTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCAccgccg           >  1:2246031/1‑93 (MQ=255)

                                                                               gTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGa          >  2:1718656/1‑93 (MQ=255)

                                                                               gTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGa          <  2:5171256/93‑1 (MQ=255)

                                                                               gTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGa          >  1:2527630/1‑93 (MQ=255)

                                                                               gTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGa          <  2:3364820/93‑1 (MQ=255)

                                                                                 aaCGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGaaa        <  2:4545843/93‑1 (MQ=255)

                                                                                  aCGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAg       >  2:4998820/1‑93 (MQ=255)

                                                                                    ggTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGcc     <  1:6068600/93‑1 (MQ=255)

                                                                                      ttCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCgg   >  2:4355854/1‑93 (MQ=255)

CCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGT  >  NC_000913/3964401‑3964580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAA  >  NC_000913/3964398‑3964583

AAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTG                                                                                              <  SRR4302534.5278498/94‑1 (MQ=60)

AAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTG                                                                                              <  SRR4302534.35196/94‑1 (MQ=60)

  ACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCACGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTCGTAACGGTTCTGCT                                                                                            >  SRR4302534.3474319/1‑94 (MQ=60)

   CCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTG                                                                                           >  SRR4302534.2398902/1‑94 (MQ=60)

    CTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGA                                                                                          <  SRR4302534.25922/94‑1 (MQ=60)

     TGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAA                                                                                         <  SRR4302534.2102156/94‑1 (MQ=60)

      GAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAG                                                                                        <  SRR4302534.4464431/94‑1 (MQ=60)

      GAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAG                                                                                        >  SRR4302534.3850386/1‑94 (MQ=60)

        AAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAT                                                                                      <  SRR4302534.1216546/94‑1 (MQ=60)

         AACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATT                                                                                     <  SRR4302534.1611150/94‑1 (MQ=60)

          ACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTT                                                                                    <  SRR4302534.1011233/94‑1 (MQ=60)

           CGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTA                                                                                   <  SRR4302534.2071423/94‑1 (MQ=60)

            GCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAA                                                                                  >  SRR4302534.4717477/1‑94 (MQ=60)

              CCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACT                                                                                >  SRR4302534.6117962/1‑94 (MQ=60)

               CGCACCAAAATCCTCTTGGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATGTAACTG                                                                               <  SRR4302534.6532028/94‑1 (MQ=60)

               CGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTG                                                                               >  SRR4302534.6276711/1‑94 (MQ=60)

               CGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTG                                                                               <  SRR4302534.3167392/94‑1 (MQ=60)

               CGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTG                                                                               >  SRR4302534.5579656/1‑94 (MQ=60)

                 CAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCT                                                                             >  SRR4302534.3341349/1‑94 (MQ=60)

                 CAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCT                                                                             >  SRR4302534.823734/1‑94 (MQ=60)

                 CAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCT                                                                             >  SRR4302534.2016245/1‑94 (MQ=60)

                  AACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTC                                                                            >  SRR4302534.4603527/1‑94 (MQ=60)

                  AACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTC                                                                            >  SRR4302534.6085899/1‑94 (MQ=60)

                     AAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCG                                                                         >  SRR4302534.3669644/1‑94 (MQ=60)

                       AATCCTCTTTGAGAACTTATCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTA                                                                       <  SRR4302534.3464743/94‑1 (MQ=60)

                       AATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTA                                                                       <  SRR4302534.1258870/94‑1 (MQ=60)

                       AATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTA                                                                       >  SRR4302534.3303444/1‑94 (MQ=60)

                        ATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTAC                                                                      >  SRR4302534.4519240/1‑94 (MQ=60)

                         TCCTCTTTGAGAACTTAGCCCGGCGGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACT                                                                     <  SRR4302534.1011831/94‑1 (MQ=60)

                           CTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGG                                                                   >  SRR4302534.5174069/1‑94 (MQ=60)

                           CTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGG                                                                   >  SRR4302534.2621724/1‑94 (MQ=60)

                           CTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATGTAACTGCTCGCGTACTGG                                                                   >  SRR4302534.1481244/1‑94 (MQ=60)

                            TCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGA                                                                  >  SRR4302534.3637497/1‑94 (MQ=60)

                            TCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGA                                                                  <  SRR4302534.1598917/94‑1 (MQ=60)

                             CTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGAT                                                                 >  SRR4302534.2231504/1‑94 (MQ=60)

                              TTTGAGAACTTACCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATC                                                                <  SRR4302534.1175727/94‑1 (MQ=60)

                              TTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATT                                                                <  SRR4302534.6632573/94‑1 (MQ=60)

                              TTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATC                                                                <  SRR4302534.1480275/94‑1 (MQ=60)

                              TATGAGAACTTATCCCCGCTGCACGCAACCTCTCGTCTGCGAATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATC                                                                <  SRR4302534.167723/94‑1 (MQ=60)

                               TTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCT                                                               >  SRR4302534.2685527/1‑94 (MQ=60)

                               TTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCT                                                               <  SRR4302534.5818713/94‑1 (MQ=60)

                               TTGAGAACTTAACCCCGCTGCACGCAAACTCTCATCTGCGTATGGAACGTGGTAACTGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCT                                                               <  SRR4302534.3224668/94‑1 (MQ=60)

                                TGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTG                                                              <  SRR4302534.4456657/94‑1 (MQ=60)

                                TGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTG                                                              <  SRR4302534.2643872/94‑1 (MQ=60)

                                 GAGAACTTACCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGG                                                             <  SRR4302534.4550314/94‑1 (MQ=60)

                                 GAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGG                                                             <  SRR4302534.2838784/94‑1 (MQ=60)

                                 GAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGG                                                             >  SRR4302534.5016547/1‑94 (MQ=60)

                                 GAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGG                                                             >  SRR4302534.6310605/1‑94 (MQ=60)

                                  AGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGC                                                            >  SRR4302534.1009806/1‑94 (MQ=60)

                                  AGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGC                                                            >  SRR4302534.944625/1‑94 (MQ=60)

                                    AACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCAT                                                          >  SRR4302534.550700/1‑94 (MQ=60)

                                     ACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATC                                                         >  SRR4302534.463057/1‑94 (MQ=60)

                                     ACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATC                                                         >  SRR4302534.4808515/1‑94 (MQ=60)

                                        TAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACC                                                      >  SRR4302534.6175307/1‑94 (MQ=60)

                                         AACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCT                                                     >  SRR4302534.377583/1‑94 (MQ=60)

                                          ACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTA                                                    >  SRR4302534.1461672/1‑94 (MQ=60)

                                          ACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGAATTAACTGCTCGCGTACTGGATCTGGCATCACCTA                                                    >  SRR4302534.5091552/1‑94 (MQ=60)

                                            CCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATC                                                  >  SRR4302534.1756455/1‑94 (MQ=60)

                                            CCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTAGGATCACCTATC                                                  >  SRR4302534.693935/1‑94 (MQ=60)

                                              CGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGG                                                >  SRR4302534.2741928/1‑94 (MQ=60)

                                                CTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTC                                              >  SRR4302534.401568/1‑94 (MQ=60)

                                                   CACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTG                                           >  SRR4302534.1051977/1‑94 (MQ=60)

                                                   CACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTG                                           >  SRR4302534.1669964/1‑94 (MQ=60)

                                                    ACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGG                                          >  SRR4302534.3755512/1‑94 (MQ=60)

                                                    ACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGG                                          >  SRR4302534.4503717/1‑94 (MQ=60)

                                                      GCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTC                                        <  SRR4302534.5554296/94‑1 (MQ=60)

                                                       CAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCA                                       >  SRR4302534.3718282/1‑94 (MQ=60)

                                                       CAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCA                                       >  SRR4302534.767039/1‑94 (MQ=60)

                                                       CAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCA                                       >  SRR4302534.5252197/1‑94 (MQ=60)

                                                         AACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGC                                     >  SRR4302534.3658190/1‑94 (MQ=60)

                                                         AACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGC                                     >  SRR4302534.737066/1‑94 (MQ=60)

                                                         AACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGC                                     >  SRR4302534.1828901/1‑94 (MQ=60)

                                                         AACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTTGATCTGGCATCACCTATCGGGCGTGGTCAGC                                     >  SRR4302534.2647485/1‑94 (MQ=60)

                                                            TCTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTG                                  <  SRR4302534.416783/94‑1 (MQ=60)

                                                             CTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGG                                 <  SRR4302534.4965428/94‑1 (MQ=60)

                                                             CTCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGG                                 <  SRR4302534.1819742/94‑1 (MQ=60)

                                                              TCGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGT                                <  SRR4302534.4898112/94‑1 (MQ=60)

                                                               CGTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTC                               >  SRR4302534.1185509/1‑94 (MQ=60)

                                                                GTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCT                              >  SRR4302534.4717303/1‑94 (MQ=60)

                                                                GTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCT                              >  SRR4302534.1540672/1‑94 (MQ=60)

                                                                GTCTGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTCGCATCACCTATCGGTCGTGGTCAGCGTGGTCT                              <  SRR4302534.5288034/94‑1 (MQ=60)

                                                                  CTTCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGA                            >  SRR4302534.274111/1‑94 (MQ=60)

                                                                  CTGCGTATGGAACGTGGTAAAGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGA                            >  SRR4302534.6449889/1‑94 (MQ=60)

                                                                   TGCGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGAT                           >  SRR4302534.4246293/1‑94 (MQ=60)

                                                                     CGTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTG                         >  SRR4302534.623575/1‑94 (MQ=60)

                                                                      GTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGT                        <  SRR4302534.1446825/94‑1 (MQ=60)

                                                                      GTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGT                        <  SRR4302534.774755/94‑1 (MQ=60)

                                                                      GTATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGT                        <  SRR4302534.4495860/94‑1 (MQ=60)

                                                                       TATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTG                       <  SRR4302534.5488586/94‑1 (MQ=60)

                                                                       TATGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTG                       <  SRR4302534.998110/94‑1 (MQ=60)

                                                                         TGGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGC                     <  SRR4302534.5970607/94‑1 (MQ=60)

                                                                          GGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCA                    >  SRR4302534.560762/1‑94 (MQ=60)

                                                                          GGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCA                    >  SRR4302534.747464/1‑94 (MQ=60)

                                                                          GGAACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCA                    <  SRR4302534.2192798/94‑1 (MQ=60)

                                                                            AGCGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACC                  >  SRR4302534.3662570/1‑94 (MQ=60)

                                                                            AACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACC                  >  SRR4302534.579631/1‑94 (MQ=60)

                                                                            AACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACC                  >  SRR4302534.5489089/1‑94 (MQ=60)

                                                                             ACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCG                 <  SRR4302534.5586885/94‑1 (MQ=60)

                                                                             ACGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCG                 >  SRR4302534.6123161/1‑94 (MQ=60)

                                                                              CGTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGC                >  SRR4302534.611878/1‑94 (MQ=60)

                                                                               GTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCCCCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCC               <  SRR4302534.2906128/94‑1 (MQ=60)

                                                                               GTGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCC               <  SRR4302534.6057561/94‑1 (MQ=60)

                                                                                TGGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCG              >  SRR4302534.2305564/1‑94 (MQ=60)

                                                                                 GGTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGA             >  SRR4302534.2594705/1‑94 (MQ=60)

                                                                                  GTAACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAA            <  SRR4302534.5099729/94‑1 (MQ=60)

                                                                                    AACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAG          >  SRR4302534.1665056/1‑94 (MQ=60)

                                                                                    AACGGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAG          >  SRR4302534.4608321/1‑94 (MQ=60)

                                                                                       GGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCG       >  SRR4302534.384557/1‑94 (MQ=60)

                                                                                       GGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCG       <  SRR4302534.6233585/94‑1 (MQ=60)

                                                                                       GGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCG       <  SRR4302534.4469547/94‑1 (MQ=60)

                                                                                       GGTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCG       >  SRR4302534.5033754/1‑94 (MQ=60)

                                                                                        GTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCCCCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGTACCGCCGAAAGCCGG      <  SRR4302534.4591535/94‑1 (MQ=60)

                                                                                        GTTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGG      >  SRR4302534.5542984/1‑94 (MQ=60)

                                                                                         TTCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGT     >  SRR4302534.4980306/1‑94 (MQ=60)

                                                                                          TCTGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTA    >  SRR4302534.4837496/1‑94 (MQ=60)

                                                                                            TGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAA  <  SRR4302534.3696518/94‑1 (MQ=60)

                                                                                            TGCTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAA  >  SRR4302534.1389292/1‑94 (MQ=60)

AAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAA  >  NC_000913/3964398‑3964583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`